RLG00000022694

DNA ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015031
Physical Location & Seq
Forward (+)
9951821 .. 9963930
12110 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022694

Sequence Viewer

Length: 4185 bp
ATGACGGCTCTAGATTCTGCGGACCTCTTCCTGACTGCCCTAACCGCCTTGTCCCACCCACCCAGACCCAGACCCCTCGAACTCTCTTTCCCTCCAATTCCCTCCACATTCCCCCACTCCAAACTCATCCCCAGAACCCGTTTCGCCGTGGACGCATTCCGCTATGCCGGCGATTATTCCGTCTCTTACTTCCTCTCTCACTTCCACTCCGATCACTACGGCGGTCTCTCCCCCAATTGGGCCCAAGGCCTCGTCTTTTGCTCCCCCACCACCGCTCGTCTTCTCACAGACGTCCTCAACGTCTCTTCGCTTTTCGTGGTTCCTCTGCCGCTCGGACAGCCCCTTCTCATTGATGGATGCGAGGTCGTGCTTATTGACGCCAATCATTGCCCAGGCGCCGTTCAGTTCTTGTTCAAAGTTCCCGTTCCCGCCTCCAATGGAGACTTGCAGAGGTATGTTCACACTGGTGATTTTCGGTTTTCTGCTTCGATGAAGTTAGACCCTTTTCTCTCTCAATTTGTGGGTTCCGATGCCATTTTCTTGGATACAACTTATTGTAATCCCAAATTCGTGTTTCCTTCACAAGAAGAATCTGTTGATTATATTTCTAGCTTGATAGAAAGAGTTGGAGGTGAACATGAAGTTTCTATGAAAAGTGTTCTGTTCCTTGTTGCTACATATGTGATTGGGAAAGAGAAGATTTTGATAGAAATTGCTCGTAGATGTAAGCGTAAAGTGTATGTGGATGCTCGAAAAATGGCGGTGCTGCGTGTTTTGGGTTACGGAGACAGTGGGGTGTTCACGGAGGATGAATGTGAGAGTGATGTGCATGTTGTTGGGTGGAATGTGTTGGGTGAGACTTGGCCATATTTCAGGCCAAATTTTGTGAAAATGGAGGAGATTATGGTTGAGAGGGGGTATTCCAGGGTTGTAGGGTTTGTCCCTACTGGCTGGACTTACGAAGTGAAGCGTAACAAGTTCTCGGTGAGGTCAAAGGATGCTTTTGAGATCCATCTTGTACCCTACAGTGAACATTCAAACTATGATGAACTTAGAGAATACGTCAGGTTTTTGAAACCAAAGCGTGTGATTCCTACTGTGGGCTCCGATGTGGAAAAGATAGACAGTAAACATGCCAGTAAAATGCAAAAGCATTTTGCTGGGTTAATTGATGAAATGGCCAACAAAAAAGAATTCTTGAGGGGTTTTCATCGCGGGACTACAGAAGGAGGTGGGAAGGTTGACAGTGATGCCAAAGATTACACGATGGAGCAAAAGGCAACACCTTCAGATATGGATACTATGGGAGATACTAATGTAGGCTTTGGTAAGGAATTGTTGTCTCCTCTTCAAGAACCTGATTCACAAACTCCAATATTGTTGACTGATGAAAAAGCAGAGGAAATTATTCAAGAACTTCGTGATTGTTTGCCGTCATGGGTCACTCGCCAGCAGATGCTAGATCTGATTGGGAGCTCAGGAGGGGACATTGTTGAAGCAGTCACTAAATTTTACGATCGTGAAACCGAATTTCATGATCAAGGAATTGCCTCTACAAGTGCAGTATCTGTATCCGAGACCAGCTCACTCTGTGACACAGCATCACCGACAAAAGCAGGTTCTGTTCATGCAAATATTGATGTTTCTTCAAGCCAAGCTCATATTTCACCCAACCCAAGAAATATTGTTAAAAGTGGCATTTCCCCTGGAAAAAGAGGGAGGAAGATCAGTAATAAAGTTAACAAGAAAGTAAAGTTACAGTCAAAATTGAATTCGTGTGGGCCAAAACAATCGACAATAACAAGGTTTTTTAGTAAAGTTTTGCCTGACGTTACAGAAAGTGCAGAGATTGGATCAGTAGGTGAACAAAACCCTAAAGATAAAAAATTACCAGATCATGTCACCCAATCATACAAGGATGCAATAGATCAATTTCTTCAGATAATAGATGGCAATGAATCATTAAAAAGCTATGCTGGTACCATTCTTCAAAAGGCAAATGGGGATATTAGTATGGCTGTGGATATACATTACCGTAATAATGAAGGCAGGTCTGATGAGAATGAAGTGGAGCTGGTGGTTGATGCAAAATCAGTTCAGTCCAACTGTGGTATAGATAACTGCTCTTCTGTCAAAAAAAAAATTGAATTAGGGAAAATAGGAGTGGTAGCTGATTTGTCTGTACAGGGGTCATTACCAGATAACGTTGCTGCAGCTTCTGTGTCACTACCACCAGAAAAATACAATCCTGTAGATCATGCATGCTGGAGCAATGGACAACATGCTCCATATTTACATCTTGCACGAACCTTTGACTTACTAGAGGATGAAAAAGGCAAGATAAAGGCTACTTCTATGCTCTGCAATATGTTCAGAAGTTTGCTTGCCCTCTCTCCTGATGATGTGCTGGCTTCAGTTTATTTGTGCACCAATAAGATAGCTGCAGACCATGAAAATGTGGAACTGAACATTGGTGGAAGTTTGGTCACAGCAGCACTGGAGGACGCATGTGGGACAAGCAGATCCAAAATCAGGGATATGTACAATGAATTGGGAGATCTTGGTGATGTTGCTCAAGCATGCCGGCAGACACAGACTTTGCTTGCCCCTCCTCCACCACTTCTAATAAAAGATGTATTTTCTGCACTCCGGAAGATAAGTGTACAAACAGGTACTGGAAGCAGTGCTCGAAAGAGAAGCCTGATTGTGAATCTCTTGCGTTCTTGCAGAGAGAAAGAGATGAAGTTCCTTGTTAGAACTTTGGTTCGAAACTTACGTATCGGAGCAATGATGAAAACTGTCCTACCTGCTCTGGCTCAAGCTGTTGTTCTGAATTCCTTCCATAGCTGTAATCACGAAGGAACTATAGAAAGTTTGAAGGACAAGCTTCAGCGCCATTCAGCAGCTGTAGTAGAAGCATACAATGTACTGCCCAATTTGGATTTGGTCGTTCCTTCTCTCATGAAAAGAGGCATTGGATTCTCCTCATCAACTTTGTCAATGGTTCCAGGAATACCTATCAAGCCAATGCTTGCAAGAATCACAAATGGTGTTCAGCAAGCACTGAAGCTATTTGAAAATAAAGCTTTTACATGTGAATACAAATATGATGGCCAGCGTGCACAAGTTCATAAACTAGTTGATGGGTCCATGCATGTTTTTACAAGAAATGGGGATGAGTCAACTTCGAGATTTCCAGATCTGATTAATATAATCAATCAATCTTGTAAACCCGATGCAGTGACTTTCATACTGGATGCAGAGGTTGTTGGAGTTGATAGGAAGAATGGTTGCAAGCTAATGTCCTTCCAAGAATTATCTTCACGTGGGAGGGGGAGCAGAGATGCATCAATAACGTTGGATAGCATAAAGGTCAACATTTGCGTCTTTGTCTTCGACATCATGTTTGCCAATGGACAACAGTTGTTAAGCTTTCCTCTGCGGAGAAGGCGAAAGTATTTGAAAGAGTTGTTCTATGATGAGAAGTTGGGTTACTTTGAATATGCCAAGGAAATGACAGTTGAGGCAGAGGATGCTTGTTTAACCAGTGAAGCGACATTAACCAAAATAAACTCTTTCCTTGAAAATGCATTTGTTTCCTCATGTGAAGGGATTATAGTTAAATCTTTAGATGTTGATGCTGGATATTCTCCATCAAAGCGTACAGATACTTGGTTAAAGGTCAAGCGAGACTACATGGAGGGATTGGGCGATACTCTTGATTTAGTCCCAATTGGAGCTTGGCATGGAAACGGGAGAAAGGCAGGCTGGTACAGTCCATTTCTCATGGCATGTTACAATCCTGAAACTGAAGAATTTCAAAGCGTCTGTCGTGTTATGTCTGGGTTTTCAGATTTATTTTATACAGAGATGAAAAGTTTTTATTCCGGGGACAAGATCTTGGCCAAAAAACCACCTTATTATAGAACAGCTGAGGTGCCTGATATGTGGTTTTCTCCTGAGCTTGTATGGGAAATAAGAGGTGCAGACTTTACAGTGTCTCCAGTTCACCAGGCTGCTTTAGGTCTAGTTCATCCATCACGTGGCATTTCCATTCGATTCCCAAGATACATTCGCACTGTGACTGATAGAAAACCAGATGAATGTAGCACAGCTTCTGATTTAGCTGAAATGTTTCATTCTCAGACGAGGAAGATGGATGTGGCTTCTGAAGATGGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000012 GO:0000726 GO:0002237 GO:0003006 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009314 GO:0009409 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010154 GO:0010225 GO:0010243 GO:0015074 GO:0016070 GO:0016874 GO:0016886 GO:0022414 GO:0022616 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0046677 GO:0048316 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051103 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0090351 GO:0140097 GO:1901360 GO:1901576 GO:1901652 GO:1901698 GO:1901700 GO:1904975 GO:2000683 GO:2000685
KEGG Pathways
Metabolic & Signaling
ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430
Pfam Domains
Protein Families
DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N DRMBL

Protein Analysis

1395

Amino Acids

154.76

Weight (kDa)

6.34

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DRMBL PF07522 265 - 368 2.4e-20 DNA repair metallo-beta-lactamase
DNA_ligase_A_N PF04675 763 - 939 3.7e-42 DNA ligase N terminus
DNA_ligase_A_M PF01068 1009 - 1229 1.4e-46 ATP dependent DNA ligase domain
DNA_ligase_A_C PF04679 1255 - 1362 1.7e-16 ATP dependent DNA ligase C terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66730
fragaria_vesca FvH4_1g29000 FvH4_1g29000 FvH4_1g29000
malus_domestica MD00G1054100.v1.1
prunus_persica Prupe.3G150300_v2.0.a1
pyrus_communis pycom17g25560
rosa_chinensis RchiOBHm_Chr3g0493691 RchiOBHm_Chr3g0493951
rosa_laevigata RLG00000022694
rosa_roxburghii Rroxscaffold_6G00391370
rosa_rugosa Rorug03G0263400 Rorug03G0263500 Rorug03G0263600
rosa_samantha Rh3AG312500 Rh3AG313200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 3 cut(s) 1607, 2040, 2815
Acc65I GGTACC 1 cut(s) 1979
AccB1I GGYRCC 3 cut(s) 395, 1979, 3937
AccB7I CCANNNNNTGG 1 cut(s) 4043
AccBSI CCGCTC 2 cut(s) 275, 331
AccII CGCG 1 cut(s) 1215
AccIII TCCGGA 1 cut(s) 2649
AclI AACGTT 2 cut(s) 2205, 3356
AclWI GGATC 3 cut(s) 1003, 1861, 2517
AcoI YGGCCR 4 cut(s) 863, 1179, 3112, 3903
AcsI RAATTY 8 cut(s) 566, 880, 1193, 1508, 1529, 1771, 2833, 3815
AcuI CTGAAG 6 cut(s) 1272, 1922, 2397, 2873, 3086, 3831
AcvI CACGTG 2 cut(s) 3326, 4043
AcyI GRCGYC 3 cut(s) 291, 378, 396
AdeI CACNNNGTG 1 cut(s) 1592
AfaI GTAC 9 cut(s) 1020, 1981, 2184, 2543, 2664, 2674, 2928, 3664, 3773
AfiI CCNNNNNNNGG 5 cut(s) 237, 238, 1100, 2532, 4043
AflIII ACRYGT 1 cut(s) 3092
AhlI ACTAGT 1 cut(s) 3136
AjnI CCWGG 5 cut(s) 391, 923, 1705, 3007, 4011
AjuI GAANNNNNNNTTGG 2 cut(s) 2454, 2486
AleI CACNNNNGTG 1 cut(s) 465
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
Alw21I GWGCWC 4 cut(s) 1478, 2429, 2689, 3124
Alw44I GTGCAC 2 cut(s) 2425, 3120
AlwI GGATC 3 cut(s) 1003, 1861, 2517
AlwNI CAGNNNCTG 6 cut(s) 1568, 1622, 2219, 2675, 2906, 4118
Aor13HI TCCGGA 1 cut(s) 2649
AoxI GGCC 8 cut(s) 240, 247, 863, 875, 1179, 1781, 3112, 3903
ApaI GGGCCC 1 cut(s) 244
ApaLI GTGCAC 2 cut(s) 2425, 3120
ApeKI GCWGC 8 cut(s) 766, 2210, 2213, 2441, 2492, 2903, 4016, 4179
ApoI RAATTY 8 cut(s) 566, 880, 1193, 1508, 1529, 1771, 2833, 3815
ArsI GACNNNNNNTTYG 6 cut(s) 2581, 2613, 3389, 3421, 3814, 3846
AseI ATTAAT 1 cut(s) 3207
Asp700I GAANNNNTTC 4 cut(s) 1407, 2837, 3816, 4134
Asp718I GGTACC 1 cut(s) 1979
AspLEI GCGC 2 cut(s) 398, 2895
AspS9I GGNCC 5 cut(s) 22, 240, 241, 1781, 3147
AsuC2I CCSGG 1 cut(s) 3889
AsuII TTCGAA 1 cut(s) 2767
AvaII GGWCC 2 cut(s) 22, 3147
BaeGI GKGCMC 3 cut(s) 244, 2429, 3124
BaeI ACNNNNGTAYC 4 cut(s) 1302, 1335, 2761, 2794
BalI TGGCCA 4 cut(s) 865, 1181, 3114, 3905
BanI GGYRCC 3 cut(s) 395, 1979, 3937
BanII GRGCYC 3 cut(s) 244, 1106, 1478
BarI GAAGNNNNNNTAC 4 cut(s) 3476, 3508, 4099, 4131
BbrPI CACGTG 2 cut(s) 3326, 4043
BbsI GAAGAC 2 cut(s) 272, 3385
Bbv12I GWGCWC 4 cut(s) 1478, 2429, 2689, 3124
BbvCI CCTCAGC 1 cut(s) 3933
BbvI GCAGC 7 cut(s) 753, 2197, 2225, 2428, 2504, 2915, 4003
BceAI ACGGC 5 cut(s) 21, 131, 235, 383, 1417
BciT130I CCWGG 5 cut(s) 393, 925, 1707, 3009, 4013
BciVI GTATCC 3 cut(s) 538, 1291, 1582
BclI TGATCA 1 cut(s) 1537
BcnI CCSGG 1 cut(s) 3889
BcuI ACTAGT 1 cut(s) 3136
BfaI CTAG 6 cut(s) 11, 609, 1460, 2321, 3137, 4028
BfmI CTRYAG 7 cut(s) 1024, 1221, 2211, 2250, 2442, 2865, 2907
BfoI RGCGCY 2 cut(s) 399, 2896
BfuAI ACCTGC 3 cut(s) 1607, 2040, 2815
BfuI GTATCC 3 cut(s) 538, 1291, 1582
BglII AGATCT 4 cut(s) 1462, 2557, 3199, 3897
BisI GCNGC 9 cut(s) 329, 767, 2211, 2214, 2442, 2493, 2904, 4017, 4180
BlsI GCNGC 9 cut(s) 330, 768, 2212, 2215, 2443, 2494, 2905, 4018, 4181
Bme1390I CCNGG 6 cut(s) 393, 925, 1707, 3009, 3889, 4013
Bme18I GGWCC 2 cut(s) 22, 3147
BmgT120I GGNCC 5 cut(s) 22, 240, 241, 1781, 3147
BmiI GGNNCC 9 cut(s) 242, 321, 397, 526, 1105, 1981, 3006, 3148, 3939
BmrFI CCNGG 6 cut(s) 393, 925, 1707, 3009, 3889, 4013
BpiI GAAGAC 2 cut(s) 272, 3385
BplI GAGNNNNNCTC 2 cut(s) 1568, 1600
BpmI CTGGAG 3 cut(s) 2287, 2519, 3987
Bpu10I CCTNAGC 3 cut(s) 1477, 3933, 3960
Bpu14I TTCGAA 1 cut(s) 2767
BpuEI CTTGAG 3 cut(s) 1219, 2559, 2802
BpuMI CCSGG 1 cut(s) 3889
BsaAI YACGTR 3 cut(s) 2777, 3326, 4043
BsaHI GRCGYC 3 cut(s) 291, 378, 396
BsaI GGTCTC 2 cut(s) 230, 1571
BsaJI CCNNGG 7 cut(s) 147, 244, 391, 924, 1705, 3507, 3888
BsaWI WCCGGW 1 cut(s) 2649
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 3985, 4015
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 238, 1100, 2532, 4043
Bse118I RCCGGY 2 cut(s) 167, 2583
Bse1I ACTGG 8 cut(s) 469, 952, 1137, 2502, 2680, 3258, 3546, 4004
Bse3DI GCAATG 4 cut(s) 385, 1960, 2278, 2793
BseAI TCCGGA 1 cut(s) 2649
BseBI CCWGG 5 cut(s) 393, 925, 1707, 3009, 4013
BseDI CCNNGG 7 cut(s) 147, 244, 391, 924, 1705, 3507, 3888
BseLI CCNNNNNNNGG 5 cut(s) 237, 238, 1100, 2532, 4043
BseMI GCAATG 4 cut(s) 385, 1960, 2278, 2793
BseMII CTCAG 4 cut(s) 1491, 3924, 3951, 4157
BseNI ACTGG 8 cut(s) 469, 952, 1137, 2502, 2680, 3258, 3546, 4004
BseRI GAGGAG 4 cut(s) 911, 1335, 2601, 2974
BseSI GKGCMC 3 cut(s) 244, 2429, 3124
BseXI GCAGC 7 cut(s) 753, 2197, 2225, 2428, 2504, 2915, 4003
BseYI CCCAGC 1 cut(s) 1160
BsgI GTGCAG 4 cut(s) 1581, 1863, 2628, 4005
Bsh1236I CGCG 1 cut(s) 1215
Bsh1285I CGRYCG 1 cut(s) 1519
BshFI GGCC 8 cut(s) 242, 249, 865, 877, 1181, 1783, 3114, 3905
BshNI GGYRCC 3 cut(s) 395, 1979, 3937
BsiEI CGRYCG 1 cut(s) 1519
BsiHKAI GWGCWC 4 cut(s) 1478, 2429, 2689, 3124
BsiSI CCGG 4 cut(s) 168, 2584, 2650, 3888
BslFI GGGAC 7 cut(s) 37, 926, 1231, 1499, 2527, 3713, 3905
BslI CCNNNNNNNGG 5 cut(s) 237, 238, 1100, 2532, 4043
BsmBI CGTCTC 2 cut(s) 187, 307
BsmFI GGGAC 7 cut(s) 37, 926, 1231, 1499, 2527, 3713, 3905
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 8 cut(s) 242, 249, 865, 877, 1181, 1783, 3114, 3905
Bso31I GGTCTC 2 cut(s) 230, 1571
Bsp119I TTCGAA 1 cut(s) 2767
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 6 cut(s) 244, 1106, 1478, 2429, 2689, 3124
Bsp13I TCCGGA 1 cut(s) 2649
Bsp1407I TGTACA 3 cut(s) 2182, 2541, 2662
BspANI GGCC 8 cut(s) 242, 249, 865, 877, 1181, 1783, 3114, 3905
BspCNI CTCAG 4 cut(s) 1490, 3925, 3952, 4156
BspEI TCCGGA 1 cut(s) 2649
BspFNI CGCG 1 cut(s) 1215
BspHI TCATGA 2 cut(s) 1534, 2961
BspLI GGNNCC 9 cut(s) 242, 321, 397, 526, 1105, 1981, 3006, 3148, 3939
BspMAI CTGCAG 2 cut(s) 2215, 2446
BspMI ACCTGC 3 cut(s) 1607, 2040, 2815
BspPI GGATC 3 cut(s) 1003, 1861, 2517
BspQI GCTCTTC 1 cut(s) 2131
BspT104I TTCGAA 1 cut(s) 2767
BspT107I GGYRCC 3 cut(s) 395, 1979, 3937
BspTNI GGTCTC 2 cut(s) 230, 1571
BsrBI CCGCTC 2 cut(s) 275, 331
BsrDI GCAATG 4 cut(s) 385, 1960, 2278, 2793
BsrFI RCCGGY 2 cut(s) 167, 2583
BsrGI TGTACA 3 cut(s) 2182, 2541, 2662
BsrI ACTGG 8 cut(s) 469, 952, 1137, 2502, 2680, 3258, 3546, 4004
BssAI RCCGGY 2 cut(s) 167, 2583
BssECI CCNNGG 7 cut(s) 147, 244, 391, 924, 1705, 3507, 3888
BssNI GRCGYC 3 cut(s) 291, 378, 396
BssT1I CCWWGG 2 cut(s) 244, 3507
Bst2UI CCWGG 5 cut(s) 393, 925, 1707, 3009, 4013
Bst6I CTCTTC 4 cut(s) 32, 310, 1353, 2131
BstACI GRCGYC 3 cut(s) 291, 378, 396
BstAPI GCANNNNNTGC 1 cut(s) 3533
BstAUI TGTACA 3 cut(s) 2182, 2541, 2662
BstBAI YACGTR 3 cut(s) 2777, 3326, 4043
BstBI TTCGAA 1 cut(s) 2767
BstDEI CTNAG 5 cut(s) 1052, 1477, 3933, 3960, 4143
BstDSI CCRYGG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 1215
BstH2I RGCGCY 2 cut(s) 399, 2896
BstHHI GCGC 2 cut(s) 398, 2895
BstMCI CGRYCG 1 cut(s) 1519
BstMWI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3448, 3533
BstNI CCWGG 5 cut(s) 393, 925, 1707, 3009, 4013
BstNSI RCATGY 9 cut(s) 833, 1136, 2265, 2285, 2511, 2583, 3096, 3158, 3795
BstSCI CCNGG 6 cut(s) 391, 923, 1705, 3007, 3887, 4011
BstSFI CTRYAG 7 cut(s) 1024, 1221, 2211, 2250, 2442, 2865, 2907
BstSLI GKGCMC 3 cut(s) 244, 2429, 3124
BstSNI TACGTA 1 cut(s) 2777
BstUI CGCG 1 cut(s) 1215
BstV1I GCAGC 7 cut(s) 753, 2197, 2225, 2428, 2504, 2915, 4003
BstV2I GAAGAC 2 cut(s) 272, 3385
BstX2I RGATCY 6 cut(s) 1008, 1462, 2522, 2557, 3199, 3897
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 6 cut(s) 1008, 1462, 2522, 2557, 3199, 3897
BsuI GTATCC 3 cut(s) 538, 1291, 1582
BsuRI GGCC 8 cut(s) 242, 249, 865, 877, 1181, 1783, 3114, 3905
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 1196
BtsI GCAGTG 2 cut(s) 2689, 3246
BveI ACCTGC 3 cut(s) 1607, 2040, 2815
CaiI CAGNNNCTG 6 cut(s) 1568, 1622, 2219, 2675, 2906, 4118
CciI TCATGA 2 cut(s) 1534, 2961
CfoI GCGC 2 cut(s) 398, 2895
Cfr10I RCCGGY 2 cut(s) 167, 2583
Cfr13I GGNCC 5 cut(s) 22, 240, 241, 1781, 3147
CseI GACGC 5 cut(s) 161, 386, 2513, 3373, 3814
Csp6I GTAC 9 cut(s) 1019, 1980, 2183, 2542, 2663, 2673, 2927, 3663, 3772
CviQI GTAC 9 cut(s) 1019, 1980, 2183, 2542, 2663, 2673, 2927, 3663, 3772
DdeI CTNAG 5 cut(s) 1052, 1477, 3933, 3960, 4143
DinI GGCGCC 1 cut(s) 397
DraIII CACNNNGTG 1 cut(s) 1592
EaeI YGGCCR 4 cut(s) 863, 1179, 3112, 3903
Eam1104I CTCTTC 4 cut(s) 32, 310, 1353, 2131
EarI CTCTTC 4 cut(s) 32, 310, 1353, 2131
Ecl136II GAGCTC 1 cut(s) 1476
Eco105I TACGTA 1 cut(s) 2777
Eco130I CCWWGG 2 cut(s) 244, 3507
Eco147I AGGCCT 1 cut(s) 249
Eco24I GRGCYC 3 cut(s) 244, 1106, 1478
Eco31I GGTCTC 2 cut(s) 230, 1571
Eco47I GGWCC 2 cut(s) 22, 3147
Eco53kI GAGCTC 1 cut(s) 1476
Eco57I CTGAAG 6 cut(s) 1272, 1922, 2397, 2873, 3086, 3831
Eco72I CACGTG 2 cut(s) 3326, 4043
EcoICRI GAGCTC 1 cut(s) 1476
EcoRI GAATTC 3 cut(s) 1193, 1771, 2833
EcoRII CCWGG 5 cut(s) 391, 923, 1705, 3007, 4011
EcoT14I CCWWGG 2 cut(s) 244, 3507
EcoT22I ATGCAT 4 cut(s) 2263, 3156, 3349, 3592
EcoT38I GRGCYC 3 cut(s) 244, 1106, 1478
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 2 cut(s) 244, 3507
Esp3I CGTCTC 2 cut(s) 187, 307
FaqI GGGAC 7 cut(s) 37, 926, 1231, 1499, 2527, 3713, 3905
FauI CCCGC 2 cut(s) 436, 1208
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 1537
Fnu4HI GCNGC 9 cut(s) 329, 767, 2211, 2214, 2442, 2493, 2904, 4017, 4180
FriOI GRGCYC 3 cut(s) 244, 1106, 1478
Fsp4HI GCNGC 9 cut(s) 329, 767, 2211, 2214, 2442, 2493, 2904, 4017, 4180
FspBI CTAG 6 cut(s) 11, 609, 1460, 2321, 3137, 4028
GlaI GCGC 2 cut(s) 397, 2894
GluI GCNGC 9 cut(s) 329, 767, 2211, 2214, 2442, 2493, 2904, 4017, 4180
GsaI CCCAGC 1 cut(s) 1164
GsuI CTGGAG 3 cut(s) 2287, 2519, 3987
HaeII RGCGCY 2 cut(s) 399, 2896
HaeIII GGCC 8 cut(s) 242, 249, 865, 877, 1181, 1783, 3114, 3905
HapII CCGG 4 cut(s) 168, 2584, 2650, 3888
HgaI GACGC 5 cut(s) 161, 386, 2513, 3373, 3814
HhaI GCGC 2 cut(s) 398, 2895
Hin1I GRCGYC 3 cut(s) 291, 378, 396
Hin6I GCGC 2 cut(s) 396, 2893
HinP1I GCGC 2 cut(s) 396, 2893
HincII GTYRAC 5 cut(s) 1243, 1383, 1741, 3183, 3376
HindII GTYRAC 5 cut(s) 1243, 1383, 1741, 3183, 3376
HindIII AAGCTT 3 cut(s) 2885, 3084, 3430
HpaI GTTAAC 1 cut(s) 1741
HpaII CCGG 4 cut(s) 168, 2584, 2650, 3888
HpyCH4IV ACGT 9 cut(s) 291, 300, 1062, 1830, 2205, 2776, 3325, 3356, 4042
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3448, 3533
HpyF3I CTNAG 5 cut(s) 1052, 1477, 3933, 3960, 4143
HpySE526I ACGT 9 cut(s) 291, 300, 1062, 1830, 2205, 2776, 3325, 3356, 4042
Hsp92I GRCGYC 3 cut(s) 291, 378, 396
HspAI GCGC 2 cut(s) 396, 2893
KasI GGCGCC 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 2649
KpnI GGTACC 1 cut(s) 1983
KroI GCCGGC 2 cut(s) 167, 2583
KroNI GCCGGC 2 cut(s) 169, 2585
Ksp22I TGATCA 1 cut(s) 1537
KspAI GTTAAC 1 cut(s) 1741
LguI GCTCTTC 1 cut(s) 2131
Lsp1109I GCAGC 7 cut(s) 753, 2197, 2225, 2428, 2504, 2915, 4003
MaeI CTAG 6 cut(s) 11, 609, 1460, 2321, 3137, 4028
MaeII ACGT 9 cut(s) 291, 300, 1062, 1830, 2205, 2776, 3325, 3356, 4042
MbiI CCGCTC 2 cut(s) 275, 331
MfeI CAATTG 2 cut(s) 235, 3732
MflI RGATCY 6 cut(s) 1008, 1462, 2522, 2557, 3199, 3897
MhlI GDGCHC 6 cut(s) 244, 1106, 1478, 2429, 2689, 3124
MlsI TGGCCA 4 cut(s) 865, 1181, 3114, 3905
MluNI TGGCCA 4 cut(s) 865, 1181, 3114, 3905
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 3188
MmeI TCCRAC 4 cut(s) 607, 2127, 3250, 3339
Mox20I TGGCCA 4 cut(s) 865, 1181, 3114, 3905
Mph1103I ATGCAT 4 cut(s) 2263, 3156, 3349, 3592
MroI TCCGGA 1 cut(s) 2649
MroNI GCCGGC 2 cut(s) 167, 2583
MroXI GAANNNNTTC 4 cut(s) 1407, 2837, 3816, 4134
MscI TGGCCA 4 cut(s) 865, 1181, 3114, 3905
MslI CAYNNNNRTG 2 cut(s) 465, 2454
Msp20I TGGCCA 4 cut(s) 865, 1181, 3114, 3905
MspA1I CMGCKG 3 cut(s) 2906, 3932, 4182
MspI CCGG 4 cut(s) 168, 2584, 2650, 3888
MspR9I CCNGG 6 cut(s) 393, 925, 1707, 3009, 3889, 4013
MunI CAATTG 2 cut(s) 235, 3732
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 5 cut(s) 393, 925, 1707, 3009, 4013
MvnI CGCG 1 cut(s) 1215
MwoI GCNNNNNNNGC 6 cut(s) 44, 152, 168, 337, 3448, 3533
NaeI GCCGGC 2 cut(s) 169, 2585
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 1 cut(s) 3889
NdeI CATATG 1 cut(s) 679
NgoMIV GCCGGC 2 cut(s) 167, 2583
NlaIV GGNNCC 9 cut(s) 242, 321, 397, 526, 1105, 1981, 3006, 3148, 3939
NmuCI GTSAC 8 cut(s) 1441, 1501, 1592, 1900, 2223, 2485, 3241, 4081
NsiI ATGCAT 4 cut(s) 2263, 3156, 3349, 3592
NspI RCATGY 9 cut(s) 833, 1136, 2265, 2285, 2511, 2583, 3096, 3158, 3795
NspV TTCGAA 1 cut(s) 2767
OliI CACNNNNGTG 1 cut(s) 465
PaeI GCATGC 2 cut(s) 2265, 2583
PagI TCATGA 2 cut(s) 1534, 2961
PceI AGGCCT 1 cut(s) 249
PciI ACATGT 1 cut(s) 3092
PciSI GCTCTTC 1 cut(s) 2131
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 2773
PctI GAATGC 1 cut(s) 155
PdiI GCCGGC 2 cut(s) 169, 2585
PdmI GAANNNNTTC 4 cut(s) 1407, 2837, 3816, 4134
PfeI GAWTC 9 cut(s) 14, 590, 1090, 1361, 1958, 2710, 2979, 3039, 4059
PflMI CCANNNNNTGG 1 cut(s) 4043
PfoI TCCNGGA 1 cut(s) 3007
PkrI GCNGC 9 cut(s) 330, 768, 2212, 2215, 2443, 2494, 2905, 4018, 4181
Ple19I CGATCG 1 cut(s) 1519
PleI GAGTC 1 cut(s) 3187
PluTI GGCGCC 1 cut(s) 399
PmaCI CACGTG 2 cut(s) 3326, 4043
PmlI CACGTG 2 cut(s) 3326, 4043
PpsI GAGTC 1 cut(s) 3187
Ppu21I YACGTR 3 cut(s) 2777, 3326, 4043
PscI ACATGT 1 cut(s) 3092
PshBI ATTAAT 1 cut(s) 3207
Psp124BI GAGCTC 1 cut(s) 1478
Psp1406I AACGTT 2 cut(s) 2205, 3356
Psp6I CCWGG 5 cut(s) 391, 923, 1705, 3007, 4011
PspCI CACGTG 2 cut(s) 3326, 4043
PspFI CCCAGC 1 cut(s) 1160
PspGI CCWGG 5 cut(s) 391, 923, 1705, 3007, 4011
PspN4I GGNNCC 9 cut(s) 242, 321, 397, 526, 1105, 1981, 3006, 3148, 3939
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 5 cut(s) 22, 240, 241, 1781, 3147
PstI CTGCAG 2 cut(s) 2215, 2446
PstNI CAGNNNCTG 6 cut(s) 1568, 1622, 2219, 2675, 2906, 4118
PsuI RGATCY 6 cut(s) 1008, 1462, 2522, 2557, 3199, 3897
PvuI CGATCG 1 cut(s) 1519
PvuII CAGCTG 3 cut(s) 2906, 3932, 4182
RsaI GTAC 9 cut(s) 1020, 1981, 2184, 2543, 2664, 2674, 2928, 3664, 3773
RsaNI GTAC 9 cut(s) 1019, 1980, 2183, 2542, 2663, 2673, 2927, 3663, 3772
RseI CAYNNNNRTG 2 cut(s) 465, 2454
SacI GAGCTC 1 cut(s) 1478
SapI GCTCTTC 1 cut(s) 2131
SatI GCNGC 9 cut(s) 329, 767, 2211, 2214, 2442, 2493, 2904, 4017, 4180
Sau96I GGNCC 5 cut(s) 22, 240, 241, 1781, 3147
SchI GAGTC 1 cut(s) 3188
ScrFI CCNGG 6 cut(s) 393, 925, 1707, 3009, 3889, 4013
SduI GDGCHC 6 cut(s) 244, 1106, 1478, 2429, 2689, 3124
SfcI CTRYAG 7 cut(s) 1024, 1221, 2211, 2250, 2442, 2865, 2907
SfoI GGCGCC 1 cut(s) 397
SfuI TTCGAA 1 cut(s) 2767
SinI GGWCC 2 cut(s) 22, 3147
SmiMI CAYNNNNRTG 2 cut(s) 465, 2454
SmlI CTYRAG 3 cut(s) 1198, 2574, 2817
SmoI CTYRAG 3 cut(s) 1198, 2574, 2817
SnaBI TACGTA 1 cut(s) 2777
SpeI ACTAGT 1 cut(s) 3136
SphI GCATGC 2 cut(s) 2265, 2583
SseBI AGGCCT 1 cut(s) 249
SspDI GGCGCC 1 cut(s) 395
SspI AATATT 3 cut(s) 1377, 1636, 1684
SspMI CTAG 6 cut(s) 11, 609, 1460, 2321, 3137, 4028
SstI GAGCTC 1 cut(s) 1478
StuI AGGCCT 1 cut(s) 249
StyD4I CCNGG 6 cut(s) 391, 923, 1705, 3007, 3887, 4011
StyI CCWWGG 2 cut(s) 244, 3507
TaiI ACGT 9 cut(s) 294, 303, 1065, 1833, 2208, 2779, 3328, 3359, 4045
TaqI TCGA 9 cut(s) 78, 488, 751, 1793, 2689, 2767, 3188, 3396, 4057
TatI WGTACW 4 cut(s) 2182, 2541, 2662, 2926
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 9 cut(s) 14, 590, 1090, 1361, 1958, 2710, 2979, 3039, 4059
TseFI GTSAC 8 cut(s) 1441, 1501, 1592, 1900, 2223, 2485, 3241, 4081
TseI GCWGC 8 cut(s) 766, 2210, 2213, 2441, 2492, 2903, 4016, 4179
Tsp45I GTSAC 8 cut(s) 1441, 1501, 1592, 1900, 2223, 2485, 3241, 4081
TspGWI ACGGA 3 cut(s) 169, 798, 818
Van91I CCANNNNNTGG 1 cut(s) 4043
VneI GTGCAC 2 cut(s) 2425, 3120
VpaK11BI GGWCC 2 cut(s) 22, 3147
VspI ATTAAT 1 cut(s) 3207
XapI RAATTY 8 cut(s) 566, 880, 1193, 1508, 1529, 1771, 2833, 3815
XbaI TCTAGA 1 cut(s) 10
XceI RCATGY 9 cut(s) 833, 1136, 2265, 2285, 2511, 2583, 3096, 3158, 3795
XcmI CCANNNNNNNNNTGG 2 cut(s) 2941, 3738
XmnI GAANNNNTTC 4 cut(s) 1407, 2837, 3816, 4134
XspI CTAG 6 cut(s) 11, 609, 1460, 2321, 3137, 4028
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 4 cut(s) 2263, 3156, 3349, 3592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.