Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004888

Physical Location & Seq

Genomic Coordinates Forward (+)

24946928 .. 24955694

Gene Structure 8767 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G66880.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3891 bp

ATGAAGAACATGAACTTTGAGCGACTCTCTGAGCTCATGGCTTTCTACCACTTGATGGGGAAGATCTCTAGTGAGTGTGGGAATATCACCGCTGGTTTCCCCTTCTGGGGAGGGAATCGTCTAAAACATTGCGGTCTTCCATCGCTGGAGCTTCACTGCAGCAAAAACATCACCTCTTTATTCATCTCAAGTCAAGAGTTCTATGTTTTACATCTAAATCAAACGTCTAAAACACTTAAACTTGCTAGAACAGACCTTTTAGGTTCAATTTGCAACTCTACTTTCACTACCATAACCTTGCCTCCAAATATTTTCGAGCTTTCTCCAACTTACAAGAGGCTCACCGTTTTCTACTACTGCTTCTTATTGACTCATTACGTTTCGAGTTATAAATGTCCTATGAGAGGTTTTATCTTTGTGTCTGAAAACCATGAATATCATAAAATCTGCGGTGACACTTTCACCGTGATTGTTCCTACACGTTTTGGTGCAGGAGAGAAAGAGTTGAATATGACCAATTTGGAAAGTGTTTTAAGTAAAGGATTTGAAGTAAAAGTGAAGATCGATGATACATCATGTCAAGAATGTTTATCCTCTCATGGAAGTTGTGGCTTTAATGAAACCTTGCCAGTGGGAGGTAAATGCAGTCCACTCAATCCAACAACACAACCACCGCCACAACCTGGGGATCGTCACAAACATTGCGGTCATCCATTGCTAGAGCTTCGCTGCGACCAAAACAAGAGTACCTCTTTATTCATCTCAGACCAAGAGTTCTTTGTTCTCCATGTCGATCAAACATCTTACAGTCTTACACTCGCCAGACCAGACCTTTTACATTCCTTTTGCTCCCTTACATTCACCAACACAACCTTACCACCAGAAATTTTCGAACTTTCTCCGGCCTACAAGAGCGTCACATTCTACCACTGTTACCCGGTTCTTCCTGACCTTTCGAATTATACATGTCCTGTTATAGGTCCAATCTCAGTGTCTGGAAACCCTGAAGATCACGAAACCTGCTTTCCCAATTTTGCCGCAAACGTTCCCACGAGTTTCGTCACGAAAGAGAAAAAATTGAATATAGCCAATTTGGAAAGTGTTTTGGAAAAGGGCTTTGAGGTGAACATGAATGTCATCATGAAAGCTTGTCAAGCATGTTCATACTCTAATGAATCCTGTGGCTTCGATGAAAACTTTCCATTCGAAGTCAAATGCAAACCACACCATTCACCAACTGCTAAGTTTCAGTGTGGGAACATCACCGCTGGTTTCCCTTTCTCCGGTGGGAATCGTCCTCAAATTTGCGGTCATCCATCGTTGGAGCTTCACTGCTATAACAACATGGCCTCTATAATCATCTCAGACCACTTTTACAATGTTCTCCATATAGATCAAACATCTAACACTCTTAGACTTGCTAGAGCAGAGCTGGAAGGTTCTTTTTGCAACGCTACTTACACAGCCACAACCTTGCCTTCCAAAATCTTTGAGATTTCATCAACCTACAAGAGCCTCACTGTTTTCTACCTCTGCGACCCTAAGGTTTCTTACCGTTCGAGTTATACATGTCCTGGTAGAGGCCTTGTCTCAGTGTCTCAAAATTCTGATTACCATAACTCCTGCCAAGACAGTTTCACGATTAACGTTCCAAAGAGCTTTGTTCCAGAAGAGAAAGAGTTGGATGTGACCAATTTGGAAAGTGCTTTAAGAGAAGGATTTGAGGTGAAGGTAAAGGTTGATGAGAAAACTTGTCAAAAATGTACATCCTCTGGTGGAACCTGCGGCTTCCAAAATTCAACACAGATTTGCTGCAAGGAAGCCTCATCATTAGGATGCAATAAAGTCCATCCACTACCTGATGCTACTGAGCTTGAGCTTCACAGACGCTGCAATGGCACGTTTAGCTGCGGCGATCAGAGAGAACTCTTTTACCCTTTCTGGACATCTGGTAGAGAAGACTGTGGCCACCCTGACTTCAAGCTCGACGACTGTAGCGGAAGATTCGCAGAGCTTAGCATTTCCTCTGTGAAATTTAGAATTCTAGCGTCGGTTTATGGCTCTAATATCATAAGACTCGGCAGATCCGAATATATAGGCGATCTCTGTCCCCAAGATCCTATAAATGCACCATTCAGCGAAAGTGTCCTTCCATTTGCTCCTAACACCGAGCTGCTGACTATTTTTTACAACTGCAGCCGAGACTTTCCACAGCAAGTTACTAATTTTGGAGACTTTGCTTGTGGGGATGATAGTGATGATGATAGAAGTTACTATGTGACGAGAAACCTCTCGTTTCCTCCACTCAGTGAGATTAATGACCTTTTATACGACTTCAGTCAATCTTGCGATAGAAACGTCAGTATTCCCGCATCTGGATCGACGCTTAACATACTGCAGAGCACTCCGAGTAACGATAATCTAAAGAAGGCTCTCGAATACGGTTTCGAGCTTGAACTTAACCAAGATTGTCGGACGTGCATAGACTCTAAGGGTGCTTGTGGATATAGTCAAACCTCAAGTAGATTCGTCTGCTATTCTATAGAAGAGCCACAAACCCCTACTCCACCAAACCCTACTAGAAACAAGGATACAAGCCTCTCTATTGGAGCAAAAGCAGGCATTGCGGTTGCTTCTGTATCGGGTTTGGCCATTCTTCTACTCGCAGGTTTGTTCTTATGCATCAGACGGAGAAGGAAGACTCAGGACGCTCAATACACAAGCAAAAGCTTGCCTATCACATCATACTCGAGTAGAGATACATCAAGAAATCCAACTTCCACAACAATCTCCTCCAGTAGCAATCACTCTCTTCTTCCCTCAATCTCTAACCTTGCAAACCGAAGCGACTACTGTGGAGTCCAAGTCTTTAGCTATGAAGAACTTGAAGAAGCAACTGAGAATTTCTCTAGAGAACTCGGGGATGGTGGCTTCGGTACTGTCTACTACGGCGTGTTGAAAGACGGACGAGCTGTAGCAGTGAAACGACTCTACGAAAGATCACTAAAACGCGTAGAACAATTCAAGAACGAAATCGAAATCTTGAAATCACTAAAACATCCGAACCTCGTGATCCTCTACGGATGCACATCAAGACACAGCAGAGAGCTTCTACTAGTCTACGAATACATCTCAAACGGAACTCTAGCCGAACATCTACACGGCAACCGCGCAGAAGCTCGTCCTCTCTGCTGGTCAACCCGATTAAACATCGCAATCGAAACCGCAAGCGCATTATCATTCCTCCACATAAAAGGAATCATACACAGAGACATCAAGACTACTAACATACTCTTAGACGATAACTACCAAGTCAAAGTCGCCGATTTCGGACTTTCACGGTTGTTTCCAATGGATCAAACACACATATCAACCGCACCACAAGGCACACCAGGCTACGTGGATCCAGAGTATTACCAATGTTACCAATTAAACGAGAAGAGCGATGTCTACAGCTTCGGAGTCGTACTAACGGAACTAATCTCATCAAAAGAAGCAGTAGACATTACTCGACACCGTCACGATATCAATCTAGCGAACATGGCTGTATCTAAGATTCAGAACAATGCATTACACGAGCTTGTGGATTCGAGTTTAGGATACGATAATGATCCTGAAGTTAGGAGGAAGATGATGGCGGTGGCGGAATTAGCGTTTCGATGTTTGCAACAAGAGAGAGATGTTAGACCGGCGATGGATGAGATTGTGGAGATTTTGAGAGGGATTAAGGATGATGAGAAGAAGCGTGTGTTGGTGAAGTCGCCGGATGTTGTGGATATTGAATGCGGTGGTGGTGATGATGTTGGATTGTTGAGGAATAGTGTTCCGCCGCCGATTTCGCCGGAGACTGATAAGTGGACTAGTAGTTCTGATACGGCGGCGAGTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010228 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0031668 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033993 GO:0036211 GO:0042221 GO:0042631 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071462 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0104004 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

1296

Amino Acids

144.43

Weight (kDa)

5.77

Isoelectric Point (pI)

49.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	33 - 96	5.1e-13	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	142 - 226	1.3e-11	Wall-associated receptor kinase C-terminal
PK_Tyr_Ser-Thr	PF07714	348 - 618	9e-50	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	349 - 616	1.9e-47	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004888)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66880 AT1G66880 AT1G66880 AT5G38210 AT5G38210 AT5G38210
fragaria_vesca	FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540
malus_domestica	MD00G1204100.v1.1 MD09G1255300.v1.1 MD17G1247800.v1.1 MD17G1248000.v1.1
prunus_persica	Prupe.3G146500_v2.0.a1 Prupe.3G146700_v2.0.a1
pyrus_communis	pycom09g17110 pycom17g24910 pycom17g25040
rosa_chinensis	RchiOBHm_Chr6g0273041
rosa_laevigata	RLG00000013645
rosa_multiflora	Rmu_ssc0000387.1_g000003
rosa_roxburghii	Rroxscaffold_7G00195700
rosa_rugosa	Rorug06G0072500
rosa_samantha	Rh6AG188100 Rh6BG191500 Rh6CG190600 Rh6DG182000
rosa_wichuraiana	Rw6G016310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	390
Acc36I	ACCTGC	3 cut(s)	1028, 1790, 2657
AccB7I	CCANNNNNTGG	2 cut(s)	55, 683
AccI	GTMKAC	4 cut(s)	2943, 3120, 3450, 3501
AccII	CGCG	2 cut(s)	3012, 3171
AclI	AACGTT	2 cut(s)	1044, 1647
AclWI	GGATC	9 cut(s)	696, 2079, 2111, 2386, 3067, 3363, 3398, 3411, 3605
AcoI	YGGCCR	2 cut(s)	1966, 2649
AcsI	RAATTY	7 cut(s)	885, 1302, 1603, 1795, 2033, 2040, 2902
AcuI	CTGAAG	3 cut(s)	1026, 2320, 3636
AdeI	CACNNNGTG	1 cut(s)	2309
AfaI	GTAC	4 cut(s)	748, 1765, 2938, 3468
AflIII	ACRYGT	4 cut(s)	479, 965, 1568, 3010
AhlI	ACTAGT	2 cut(s)	3115, 3860
AjiI	CACGTC	1 cut(s)	2478
AjnI	CCWGG	3 cut(s)	682, 1573, 3391
AjuI	GAANNNNNNNTTGG	2 cut(s)	3734, 3766
AloI	GAACNNNNNNTCC	2 cut(s)	3850, 3882
Alw21I	GWGCWC	2 cut(s)	36, 2405
Alw26I	GTCTC	6 cut(s)	1594, 1602, 2196, 2226, 3264, 3839
AlwI	GGATC	9 cut(s)	696, 2079, 2111, 2386, 3067, 3363, 3398, 3411, 3605
AlwNI	CAGNNNCTG	2 cut(s)	995, 1890
Ama87I	CYCGRG	2 cut(s)	2749, 2918
AoxI	GGCC	5 cut(s)	903, 1347, 1582, 1966, 2649
ApeKI	GCWGC	7 cut(s)	159, 729, 1812, 1890, 1908, 2173, 2196
ApoI	RAATTY	7 cut(s)	885, 1302, 1603, 1795, 2033, 2040, 2902
ArsI	GACNNNNNNTTYG	2 cut(s)	1294, 1326
AseI	ATTAAT	1 cut(s)	2316
Asp700I	GAANNNNTTC	1 cut(s)	1197
AspLEI	GCGC	2 cut(s)	3173, 3233
AspS9I	GGNCC	1 cut(s)	980
AsuC2I	CCSGG	1 cut(s)	938
AsuII	TTCGAA	3 cut(s)	891, 956, 1206
AvaI	CYCGRG	2 cut(s)	2749, 2918
AvaII	GGWCC	1 cut(s)	980
AxyI	CCTNAGG	1 cut(s)	1542
BaeI	ACNNNNGTAYC	2 cut(s)	730, 763
BalI	TGGCCA	2 cut(s)	1968, 2651
BamHI	GGATCC	1 cut(s)	3403
BanII	GRGCYC	1 cut(s)	36
BauI	CACGAG	3 cut(s)	1051, 3068, 3575
BbsI	GAAGAC	3 cut(s)	128, 1965, 2705
Bbv12I	GWGCWC	2 cut(s)	36, 2405
BbvI	GCAGC	7 cut(s)	171, 716, 1799, 1877, 1895, 2160, 2208
BccI	CCATC	7 cut(s)	49, 148, 1324, 1857, 2918, 3628, 3688
BceAI	ACGGC	2 cut(s)	2965, 3178
BcgI	CGANNNNNNTGC	2 cut(s)	2361, 2395
BciT130I	CCWGG	3 cut(s)	684, 1575, 3393
BciVI	GTATCC	2 cut(s)	2584, 3593
BcnI	CCSGG	1 cut(s)	938
BcoDI	GTCTC	6 cut(s)	1594, 1602, 2196, 2226, 3264, 3839
BcuI	ACTAGT	2 cut(s)	3115, 3860
BfmI	CTRYAG	7 cut(s)	157, 1993, 2194, 2396, 2541, 2973, 3451
BfuAI	ACCTGC	3 cut(s)	1028, 1790, 2657
BfuI	GTATCC	2 cut(s)	2584, 3593
BglII	AGATCT	1 cut(s)	63
BlpI	GCTNAGC	1 cut(s)	2015
Bme1390I	CCNGG	4 cut(s)	684, 938, 1575, 3393
Bme18I	GGWCC	1 cut(s)	980
BmeT110I	CYCGRG	2 cut(s)	2749, 2918
BmgBI	CACGTC	1 cut(s)	2478
BmgT120I	GGNCC	1 cut(s)	980
BmiI	GGNNCC	2 cut(s)	1780, 3405
BmrFI	CCNGG	4 cut(s)	684, 938, 1575, 3393
BmsI	GCATC	5 cut(s)	1826, 1852, 2381, 2691, 3074
BoxI	GACNNNNGTC	1 cut(s)	2337
BpiI	GAAGAC	3 cut(s)	128, 1965, 2705
BplI	GAGNNNNNCTC	4 cut(s)	11, 43, 2891, 2923
BpmI	CTGGAG	2 cut(s)	167, 2779
Bpu1102I	GCTNAGC	1 cut(s)	2015
Bpu14I	TTCGAA	3 cut(s)	891, 956, 1206
BpuEI	CTTGAG	3 cut(s)	172, 1895, 2503
BpuMI	CCSGG	1 cut(s)	938
Bsa29I	ATCGAT	1 cut(s)	564
BsaAI	YACGTR	1 cut(s)	3400
BsaBI	GATNNNNATC	3 cut(s)	3088, 3528, 3609
BsaJI	CCNNGG	1 cut(s)	683
BsaWI	WCCGGW	1 cut(s)	1283
BsaXI	ACNNNNNCTCC	8 cut(s)	286, 316, 1604, 1634, 2548, 2578, 2850, 2880
Bse118I	RCCGGY	1 cut(s)	3688
Bse1I	ACTGG	2 cut(s)	629, 2796
Bse21I	CCTNAGG	1 cut(s)	1542
Bse3DI	GCAATG	5 cut(s)	127, 700, 713, 1900, 2622
Bse8I	GATNNNNATC	3 cut(s)	3088, 3528, 3609
BseBI	CCWGG	3 cut(s)	684, 1575, 3393
BseCI	ATCGAT	1 cut(s)	564
BseDI	CCNNGG	1 cut(s)	683
BseJI	GATNNNNATC	3 cut(s)	3088, 3528, 3609
BseMI	GCAATG	5 cut(s)	127, 700, 713, 1900, 2622
BseMII	CTCAG	9 cut(s)	21, 777, 1002, 1377, 1605, 1860, 2320, 2717, 2889
BseNI	ACTGG	2 cut(s)	629, 2796
BseRI	GAGGAG	1 cut(s)	2782
BseXI	GCAGC	7 cut(s)	171, 716, 1799, 1877, 1895, 2160, 2208
BsgI	GTGCAG	1 cut(s)	510
Bsh1236I	CGCG	2 cut(s)	3012, 3171
BshFI	GGCC	5 cut(s)	905, 1349, 1584, 1968, 2651
BshVI	ATCGAT	1 cut(s)	564
BsiHKAI	GWGCWC	2 cut(s)	36, 2405
BsiHKCI	CYCGRG	2 cut(s)	2749, 2918
BsiSI	CCGG	6 cut(s)	902, 938, 1284, 3689, 3764, 3842
BslFI	GGGAC	1 cut(s)	2094
BsmAI	GTCTC	6 cut(s)	1594, 1602, 2196, 2226, 3264, 3839
BsmFI	GGGAC	1 cut(s)	2094
BsmI	GAATGC	1 cut(s)	3788
BsnI	GGCC	5 cut(s)	905, 1349, 1584, 1968, 2651
BsoBI	CYCGRG	2 cut(s)	2749, 2918
Bsp119I	TTCGAA	3 cut(s)	891, 956, 1206
Bsp1286I	GDGCHC	2 cut(s)	36, 2405
Bsp1407I	TGTACA	1 cut(s)	1763
Bsp1720I	GCTNAGC	1 cut(s)	2015
BspANI	GGCC	5 cut(s)	905, 1349, 1584, 1968, 2651
BspCNI	CTCAG	9 cut(s)	22, 776, 1001, 1376, 1604, 1861, 2319, 2716, 2890
BspDI	ATCGAT	1 cut(s)	564
BspFNI	CGCG	2 cut(s)	3012, 3171
BspHI	TCATGA	1 cut(s)	1140
BspLI	GGNNCC	2 cut(s)	1780, 3405
BspMAI	CTGCAG	3 cut(s)	161, 2198, 2400
BspMI	ACCTGC	3 cut(s)	1028, 1790, 2657
BspPI	GGATC	9 cut(s)	696, 2079, 2111, 2386, 3067, 3363, 3398, 3411, 3605
BspQI	GCTCTTC	2 cut(s)	2541, 3434
BspT104I	TTCGAA	3 cut(s)	891, 956, 1206
BsrDI	GCAATG	5 cut(s)	127, 700, 713, 1900, 2622
BsrFI	RCCGGY	1 cut(s)	3688
BsrGI	TGTACA	1 cut(s)	1763
BsrI	ACTGG	2 cut(s)	629, 2796
BssAI	RCCGGY	1 cut(s)	3688
BssECI	CCNNGG	1 cut(s)	683
BssSI	CACGAG	3 cut(s)	1051, 3068, 3575
Bst2BI	CACGAG	3 cut(s)	1051, 3068, 3575
Bst2UI	CCWGG	3 cut(s)	684, 1575, 3393
Bst6I	CTCTTC	4 cut(s)	1665, 2541, 2817, 3434
BstAPI	GCANNNNNTGC	1 cut(s)	2624
BstAUI	TGTACA	1 cut(s)	1763
BstBAI	YACGTR	1 cut(s)	3400
BstBI	TTCGAA	3 cut(s)	891, 956, 1206
BstC8I	GCNNGC	3 cut(s)	2620, 2732, 3229
BstENI	CCTNNNNNAGG	3 cut(s)	402, 975, 1578
BstFNI	CGCG	2 cut(s)	3012, 3171
BstHHI	GCGC	2 cut(s)	3173, 3233
BstMAI	GTCTC	6 cut(s)	1594, 1602, 2196, 2226, 3264, 3839
BstMWI	GCNNNNNNNGC	8 cut(s)	1154, 1896, 1905, 2624, 3393, 3542, 3650, 3838
BstNI	CCWGG	3 cut(s)	684, 1575, 3393
BstNSI	RCATGY	3 cut(s)	969, 1161, 1572
BstPAI	GACNNNNGTC	1 cut(s)	2337
BstSCI	CCNGG	4 cut(s)	682, 936, 1573, 3391
BstSFI	CTRYAG	7 cut(s)	157, 1993, 2194, 2396, 2541, 2973, 3451
BstUI	CGCG	2 cut(s)	3012, 3171
BstV1I	GCAGC	7 cut(s)	171, 716, 1799, 1877, 1895, 2160, 2208
BstV2I	GAAGAC	3 cut(s)	128, 1965, 2705
BstX2I	RGATCY	4 cut(s)	63, 2084, 2116, 3403
BstYI	RGATCY	4 cut(s)	63, 2084, 2116, 3403
Bsu15I	ATCGAT	1 cut(s)	564
Bsu36I	CCTNAGG	1 cut(s)	1542
BsuI	GTATCC	2 cut(s)	2584, 3593
BsuRI	GGCC	5 cut(s)	905, 1349, 1584, 1968, 2651
BsuTUI	ATCGAT	1 cut(s)	564
BtgZI	GCGATG	4 cut(s)	126, 3196, 3459, 3707
BtrI	CACGTC	1 cut(s)	2478
BtsI	GCAGTG	3 cut(s)	154, 1330, 2985
BveI	ACCTGC	3 cut(s)	1028, 1790, 2657
Cac8I	GCNNGC	3 cut(s)	2620, 2732, 3229
CaiI	CAGNNNCTG	2 cut(s)	995, 1890
CciI	TCATGA	1 cut(s)	1140
CfoI	GCGC	2 cut(s)	3173, 3233
Cfr10I	RCCGGY	1 cut(s)	3688
Cfr13I	GGNCC	1 cut(s)	980
ClaI	ATCGAT	1 cut(s)	564
CseI	GACGC	5 cut(s)	904, 1896, 2037, 2392, 2717
Csp6I	GTAC	4 cut(s)	747, 1764, 2937, 3467
CviQI	GTAC	4 cut(s)	747, 1764, 2937, 3467
DraIII	CACNNNGTG	1 cut(s)	2309
EaeI	YGGCCR	2 cut(s)	1966, 2649
Eam1104I	CTCTTC	4 cut(s)	1665, 2541, 2817, 3434
EarI	CTCTTC	4 cut(s)	1665, 2541, 2817, 3434
EciI	GGCGGA	2 cut(s)	3659, 3816
Ecl136II	GAGCTC	1 cut(s)	34
Eco147I	AGGCCT	1 cut(s)	1584
Eco24I	GRGCYC	1 cut(s)	36
Eco32I	GATATC	1 cut(s)	3526
Eco47I	GGWCC	1 cut(s)	980
Eco53kI	GAGCTC	1 cut(s)	34
Eco57I	CTGAAG	3 cut(s)	1026, 2320, 3636
Eco81I	CCTNAGG	1 cut(s)	1542
Eco88I	CYCGRG	2 cut(s)	2749, 2918
EcoICRI	GAGCTC	1 cut(s)	34
EcoNI	CCTNNNNNAGG	3 cut(s)	402, 975, 1578
EcoRI	GAATTC	1 cut(s)	2040
EcoRII	CCWGG	3 cut(s)	682, 1573, 3391
EcoRV	GATATC	1 cut(s)	3526
EcoT22I	ATGCAT	2 cut(s)	2684, 3571
EcoT38I	GRGCYC	1 cut(s)	36
FaqI	GGGAC	1 cut(s)	2094
FauI	CCCGC	1 cut(s)	2377
FblI	GTMKAC	4 cut(s)	2943, 3120, 3450, 3501
FriOI	GRGCYC	1 cut(s)	36
GlaI	GCGC	2 cut(s)	3172, 3232
GsuI	CTGGAG	2 cut(s)	167, 2779
HaeIII	GGCC	5 cut(s)	905, 1349, 1584, 1968, 2651
HapII	CCGG	6 cut(s)	902, 938, 1284, 3689, 3764, 3842
HgaI	GACGC	5 cut(s)	904, 1896, 2037, 2392, 2717
HhaI	GCGC	2 cut(s)	3173, 3233
Hin6I	GCGC	2 cut(s)	3171, 3231
HinP1I	GCGC	2 cut(s)	3171, 3231
HincII	GTYRAC	1 cut(s)	3198
HindII	GTYRAC	1 cut(s)	3198
HindIII	AAGCTT	2 cut(s)	1146, 2728
HpaII	CCGG	6 cut(s)	902, 938, 1284, 3689, 3764, 3842
Hpy166II	GTNNAC	8 cut(s)	650, 1126, 2944, 3121, 3198, 3451, 3502, 3858
Hpy8I	GTNNAC	8 cut(s)	650, 1126, 2944, 3121, 3198, 3451, 3502, 3858
Hpy99I	CGWCG	3 cut(s)	1991, 2053, 2386
HpyAV	CCTTC	8 cut(s)	112, 1430, 1488, 1709, 1723, 2159, 2422, 2688
HpyCH4IV	ACGT	9 cut(s)	224, 378, 481, 1044, 1647, 1901, 2358, 2477, 3399
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1154, 1896, 1905, 2624, 3393, 3542, 3650, 3838
HpySE526I	ACGT	9 cut(s)	224, 378, 481, 1044, 1647, 1901, 2358, 2477, 3399
HspAI	GCGC	2 cut(s)	3171, 3231
LguI	GCTCTTC	2 cut(s)	2541, 3434
LmnI	GCTCC	5 cut(s)	148, 854, 1324, 2164, 2609
Lsp1109I	GCAGC	7 cut(s)	171, 716, 1799, 1877, 1895, 2160, 2208
LweI	GCATC	5 cut(s)	1826, 1852, 2381, 2691, 3074
MaeII	ACGT	9 cut(s)	224, 378, 481, 1044, 1647, 1901, 2358, 2477, 3399
MflI	RGATCY	4 cut(s)	63, 2084, 2116, 3403
MhlI	GDGCHC	2 cut(s)	36, 2405
MlsI	TGGCCA	2 cut(s)	1968, 2651
MluI	ACGCGT	1 cut(s)	3010
MluNI	TGGCCA	2 cut(s)	1968, 2651
MlyI	GAGTC	8 cut(s)	18, 364, 2070, 2480, 2695, 2868, 2982, 3471
MmeI	TCCRAC	7 cut(s)	350, 683, 1302, 1662, 2453, 2798, 3784
Mox20I	TGGCCA	2 cut(s)	1968, 2651
Mph1103I	ATGCAT	2 cut(s)	2684, 3571
MroXI	GAANNNNTTC	1 cut(s)	1197
MscI	TGGCCA	2 cut(s)	1968, 2651
MslI	CAYNNNNRTG	1 cut(s)	1834
Msp20I	TGGCCA	2 cut(s)	1968, 2651
MspA1I	CMGCKG	2 cut(s)	92, 1268
MspI	CCGG	6 cut(s)	902, 938, 1284, 3689, 3764, 3842
MspR9I	CCNGG	4 cut(s)	684, 938, 1575, 3393
Mva1269I	GAATGC	1 cut(s)	3788
MvaI	CCWGG	3 cut(s)	684, 1575, 3393
MvnI	CGCG	2 cut(s)	3012, 3171
MwoI	GCNNNNNNNGC	8 cut(s)	1154, 1896, 1905, 2624, 3393, 3542, 3650, 3838
NciI	CCSGG	1 cut(s)	938
NlaIV	GGNNCC	2 cut(s)	1780, 3405
NmeAIII	GCCGAG	2 cut(s)	2058, 2225
NmuCI	GTSAC	7 cut(s)	452, 692, 916, 1060, 1687, 2278, 3518
NsiI	ATGCAT	2 cut(s)	2684, 3571
NspI	RCATGY	3 cut(s)	969, 1161, 1572
NspV	TTCGAA	3 cut(s)	891, 956, 1206
PaeR7I	CTCGAG	1 cut(s)	2749
PagI	TCATGA	1 cut(s)	1140
PceI	AGGCCT	1 cut(s)	1584
PciI	ACATGT	2 cut(s)	965, 1568
PciSI	GCTCTTC	2 cut(s)	2541, 3434
PcsI	WCGNNNNNNNCGW	2 cut(s)	2085, 3441
PctI	GAATGC	1 cut(s)	3788
PdmI	GAANNNNTTC	1 cut(s)	1197
PfeI	GAWTC	8 cut(s)	115, 1175, 1291, 2004, 2526, 3258, 3556, 3587
PflFI	GACNNNGTC	1 cut(s)	3516
PflMI	CCANNNNNTGG	2 cut(s)	55, 683
PleI	GAGTC	8 cut(s)	18, 364, 2070, 2480, 2695, 2867, 2982, 3470
PpsI	GAGTC	8 cut(s)	18, 364, 2070, 2480, 2695, 2867, 2982, 3470
Ppu21I	YACGTR	1 cut(s)	3400
PscI	ACATGT	2 cut(s)	965, 1568
PshAI	GACNNNNGTC	1 cut(s)	2337
PshBI	ATTAAT	1 cut(s)	2316
PsiI	TTATAA	1 cut(s)	390
Psp124BI	GAGCTC	1 cut(s)	36
Psp1406I	AACGTT	2 cut(s)	1044, 1647
Psp6I	CCWGG	3 cut(s)	682, 1573, 3391
PspGI	CCWGG	3 cut(s)	682, 1573, 3391
PspN4I	GGNNCC	2 cut(s)	1780, 3405
PspPI	GGNCC	1 cut(s)	980
PspXI	VCTCGAGB	1 cut(s)	2749
PstI	CTGCAG	3 cut(s)	161, 2198, 2400
PstNI	CAGNNNCTG	2 cut(s)	995, 1890
PsuI	RGATCY	4 cut(s)	63, 2084, 2116, 3403
PsyI	GACNNNGTC	1 cut(s)	3516
RsaI	GTAC	4 cut(s)	748, 1765, 2938, 3468
RsaNI	GTAC	4 cut(s)	747, 1764, 2937, 3467
RseI	CAYNNNNRTG	1 cut(s)	1834
SacI	GAGCTC	1 cut(s)	36
SapI	GCTCTTC	2 cut(s)	2541, 3434
Sau96I	GGNCC	1 cut(s)	980
SchI	GAGTC	8 cut(s)	18, 364, 2070, 2480, 2695, 2868, 2982, 3471
ScrFI	CCNGG	4 cut(s)	684, 938, 1575, 3393
SduI	GDGCHC	2 cut(s)	36, 2405
SfaNI	GCATC	5 cut(s)	1826, 1852, 2381, 2691, 3074
SfcI	CTRYAG	7 cut(s)	157, 1993, 2194, 2396, 2541, 2973, 3451
Sfr274I	CTCGAG	1 cut(s)	2749
SfuI	TTCGAA	3 cut(s)	891, 956, 1206
SinI	GGWCC	1 cut(s)	980
SlaI	CTCGAG	1 cut(s)	2749
SmiMI	CAYNNNNRTG	1 cut(s)	1834
SmlI	CTYRAG	4 cut(s)	187, 1874, 2518, 2749
SmoI	CTYRAG	4 cut(s)	187, 1874, 2518, 2749
SpeI	ACTAGT	2 cut(s)	3115, 3860
SseBI	AGGCCT	1 cut(s)	1584
SspI	AATATT	1 cut(s)	310
SstI	GAGCTC	1 cut(s)	36
StuI	AGGCCT	1 cut(s)	1584
StyD4I	CCNGG	4 cut(s)	682, 936, 1573, 3391
TaiI	ACGT	9 cut(s)	227, 381, 484, 1047, 1650, 1904, 2361, 2480, 3402
TatI	WGTACW	1 cut(s)	1763
TauI	GCSGC	5 cut(s)	1040, 1788, 1914, 3832, 3881
TfiI	GAWTC	8 cut(s)	115, 1175, 1291, 2004, 2526, 3258, 3556, 3587
TseFI	GTSAC	7 cut(s)	452, 692, 916, 1060, 1687, 2278, 3518
TseI	GCWGC	7 cut(s)	159, 729, 1812, 1890, 1908, 2173, 2196
Tsp45I	GTSAC	7 cut(s)	452, 692, 916, 1060, 1687, 2278, 3518
TspGWI	ACGGA	5 cut(s)	2704, 2979, 3096, 3153, 3488
Tth111I	GACNNNGTC	1 cut(s)	3516
Van91I	CCANNNNNTGG	2 cut(s)	55, 683
VpaK11BI	GGWCC	1 cut(s)	980
VspI	ATTAAT	1 cut(s)	2316
XagI	CCTNNNNNAGG	3 cut(s)	402, 975, 1578
XapI	RAATTY	7 cut(s)	885, 1302, 1603, 1795, 2033, 2040, 2902
XbaI	TCTAGA	1 cut(s)	2909
XceI	RCATGY	3 cut(s)	969, 1161, 1572
XhoI	CTCGAG	1 cut(s)	2749
XmiI	GTMKAC	4 cut(s)	2943, 3120, 3450, 3501
XmnI	GAANNNNTTC	1 cut(s)	1197
Zsp2I	ATGCAT	2 cut(s)	2684, 3571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.