Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004888

Physical Location & Seq

Genomic Coordinates Forward (+)

11013309 .. 11021960

Gene Structure 8652 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g12540.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGAATATGGATATGGAGGGACCTAATACCCTAGGCTTCTTCGGCTACACCCTCCTCAGCCTACCTATTACCTGCGGGAGCGTCAAAAGCAACATCTCCTACCCCTTTTGGGGGTCCAACCGACCCGAATACTGTGGTAAATCTGGGTTTGAGGTCACATGCAACAACACTGTCCCGATGATCACCATGAAGAAAATCAATTTCAGAATTCTTGACATGAGCAATAGCACTACTACACCAACTCCGACTGTGACAGTTGCAAGGGAGGATTACTGGAATACTCTCTGTCCTCCGAGTTATATTGACACAGACCTCAATTTCTCTCTATTTGAGTATGCATCTGGCCCTCAAAACGTGTCTTTTTACTACGGATGCAATGCAGCTGGGAGTTCAGAGTTGTCACTTTTTACTAGTATCACGCATGAATGCAGTAGTACAGTGACCGTTTCCTTTCTCACAGAGAATCAAATTGCTAAGCTTCCTAATCCAACTCTCTTGGCCTCCTGTGCACCTGTGGTTGTGGTTCCAGTGTCTGCGGCAGCTGCTGAAGCACTTGACAAAAATGAAACATCAACTATCCAGAGTGCGGTAGATGGTGGTTTTGAATTGAATGTGCAGGATGATGATACTGTTCTTTGCAACGACTGTGTGGGCTCAGGAGGATTTTGTGGGCAAGACACTACTAATGCTGAGTTCATTTGCTATTGCCAAAATAGCACTTCAGCAAGCACATGTGTTTCAACTTCAGCACCTCCAAATTCAGCCACCAATTCATCAACAGGAAAGTCTACAAGTATAAAAATAGGTTTTGCTGTTGGAGGTGCAATCCTTTTTGGTATTTTCGTGGGATTCTATGTATATTATTGCCTCCAGCGAAAGAAGAAACGTGCAGCAGAAGCTCAAAGCAAAGAGATTCCCACGCCACTTACAAGCAAAAGCACCGCTCCTCCCTCGACTAGTCTCTCACAATTTGAGAGCATCCCTTCTTATCCTTCCTTCACCTCAAAATCAAGGTCCGACCTTGGGAAAGCCAGCACTTACTTTGGAGTTCAGGTCTTCAGCTATGCTGAATTAGAGGAAGCCACCGAAAATTTCAACCCTGCTAAAGAACTCGGTGATGGAGGCTTTGGCACCGTTTACTATGGTAAGCTACATGATGGTCGTGTAGTTGCTGTGAAGCGCCTTTATGAAAACAATTTCAAACGGGTGGAGCAGTTTATGAATGAGGTTGAGATCTTAACACGTCTCGAACACCAGAATCTTGTGAAGCTGTATGGATGCACCTCAAGACGTAGCCGAGAACTCCTCCTTGTCTATGAATATATTTCTAACGGAACAGTGGCTGACCATCTCCATGGAAAACGAAAGGAATCTGGCTTTCTCAGCTGGCCTGTTAGATTGAGCATCGCCATAGAGACAGCTGATGCACTAACTTTCCTTCATCGCAATGATGTGATACACCGTGATGTCAAAACCAACAATATACTCCTGGATGACGAATTTTGTGTGAAAGTTGCTGATTTTGGTCTGTCACGGTTGTTTCCCAATGATCAGACCCATGTGTCAACTGCTCCACAAGGGACGCCTGGCTATGTTGATCCCGAGTATTACCAGTGTTATCAACTCACTGACAAAAGTGATGTTTATAGCTTTGGGGTGGTCTTGATCGAGCTTATATCATCCTTACAAGCTGTGGACACCAACAGGCACCGGCATGATATAAATTTGGCTAACTTGGCTATCAACAAAATTCAAAATCATTTAGTGAATGAGTTGGTTGACCCTCTACTTGGGTATGACAAAGACTATTCTGTGAGGATAATGGCAACAGCTGTTGCAGAATTGGCCTTCCGGTGTTTGCAGCAGGAGAAAGATATGAGACCAACCATGGAAGAAGTACTGGAGGGTTTGAAAGCTATCCAAAATGAAGATCTTGGTGCACAAGAAAATGGGGTGGCAGAGGCAGTGGTCCTGGATATAGGAGCCGATGAAGTTGGCCTTTTGAGGAATTTGCCTCCCCCGCTCTCACCAGACTCAGTTGTCACTGATAAATTGATTTTTAAAAGGTGGGACAGAACTATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

695

Amino Acids

76.34

Weight (kDa)

5.0

Isoelectric Point (pI)

33.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	35 - 99	1.3e-12	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	176 - 247	5.1e-10	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	360 - 628	1.4e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	361 - 629	9e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004888)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66880 AT1G66880 AT1G66880 AT5G38210 AT5G38210 AT5G38210
fragaria_vesca	FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540
malus_domestica	MD00G1204100.v1.1 MD09G1255300.v1.1 MD17G1247800.v1.1 MD17G1248000.v1.1
prunus_persica	Prupe.3G146500_v2.0.a1 Prupe.3G146700_v2.0.a1
pyrus_communis	pycom09g17110 pycom17g24910 pycom17g25040
rosa_chinensis	RchiOBHm_Chr6g0273041
rosa_laevigata	RLG00000013645
rosa_multiflora	Rmu_ssc0000387.1_g000003
rosa_roxburghii	Rroxscaffold_7G00195700
rosa_rugosa	Rorug06G0072500
rosa_samantha	Rh6AG188100 Rh6BG191500 Rh6CG190600 Rh6DG182000
rosa_wichuraiana	Rw6G016310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1561, 2039
Acc36I	ACCTGC	1 cut(s)	80
AccB1I	GGYRCC	2 cut(s)	1130, 1707
AccBSI	CCGCTC	2 cut(s)	944, 2023
AccI	GTMKAC	1 cut(s)	788
AciI	CCGC	5 cut(s)	75, 536, 587, 942, 2021
AclWI	GGATC	1 cut(s)	1592
AcsI	RAATTY	7 cut(s)	207, 757, 1090, 1499, 1723, 1749, 2008
AcuI	CTGAAG	4 cut(s)	567, 705, 729, 1042
AcyI	GRCGYC	1 cut(s)	1583
AfaI	GTAC	2 cut(s)	436, 1899
AfiI	CCNNNNNNNGG	6 cut(s)	109, 110, 111, 586, 1023, 1790
AflIII	ACRYGT	3 cut(s)	354, 731, 1241
AgsI	TTSAA	7 cut(s)	605, 610, 741, 1096, 1201, 1754, 1912
AhlI	ACTAGT	2 cut(s)	410, 956
AjiI	CACGTC	1 cut(s)	1244
AjnI	CCWGG	3 cut(s)	1488, 1585, 1971
Alw21I	GWGCWC	2 cut(s)	511, 1942
Alw26I	GTCTC	4 cut(s)	965, 1250, 1409, 1873
Alw44I	GTGCAC	2 cut(s)	507, 1938
AlwI	GGATC	1 cut(s)	1592
AlwNI	CAGNNNCTG	3 cut(s)	533, 545, 1343
Ama87I	CYCGRG	1 cut(s)	1601
AoxI	GGCC	5 cut(s)	343, 498, 1388, 1845, 1996
ApaLI	GTGCAC	2 cut(s)	507, 1938
ApeKI	GCWGC	5 cut(s)	380, 539, 542, 890, 1861
ApoI	RAATTY	7 cut(s)	207, 757, 1090, 1499, 1723, 1749, 2008
ArsI	GACNNNNNNTTYG	2 cut(s)	998, 1030
AspA2I	CCTAGG	1 cut(s)	31
AspLEI	GCGC	1 cut(s)	1182
AspS9I	GGNCC	5 cut(s)	20, 114, 344, 1014, 1969
AsuHPI	GGTGA	4 cut(s)	175, 991, 1127, 2019
AvaI	CYCGRG	1 cut(s)	1601
AvaII	GGWCC	4 cut(s)	20, 114, 1014, 1969
AvrII	CCTAGG	1 cut(s)	31
BaeGI	GKGCMC	2 cut(s)	511, 1942
BanI	GGYRCC	2 cut(s)	1130, 1707
BanII	GRGCYC	1 cut(s)	656
BbsI	GAAGAC	1 cut(s)	1048
Bbv12I	GWGCWC	2 cut(s)	511, 1942
BbvCI	CCTCAGC	1 cut(s)	56
BbvI	GCAGC	5 cut(s)	392, 529, 551, 902, 1873
BccI	CCATC	4 cut(s)	587, 1112, 1151, 1356
BciT130I	CCWGG	3 cut(s)	1490, 1587, 1973
BclI	TGATCA	2 cut(s)	180, 1549
BcoDI	GTCTC	4 cut(s)	965, 1250, 1409, 1873
BcuI	ACTAGT	2 cut(s)	410, 956
BfaI	CTAG	3 cut(s)	32, 411, 957
BfoI	RGCGCY	1 cut(s)	1183
BfuAI	ACCTGC	1 cut(s)	80
BglII	AGATCT	2 cut(s)	1233, 1930
BisI	GCNGC	6 cut(s)	381, 537, 540, 543, 891, 1862
BlnI	CCTAGG	1 cut(s)	31
BlpI	GCTNAGC	1 cut(s)	474
BlsI	GCNGC	6 cut(s)	382, 538, 541, 544, 892, 1863
BmcAI	AGTACT	1 cut(s)	1899
Bme1390I	CCNGG	3 cut(s)	1490, 1587, 1973
Bme18I	GGWCC	4 cut(s)	20, 114, 1014, 1969
BmeT110I	CYCGRG	1 cut(s)	1601
BmgBI	CACGTC	1 cut(s)	1244
BmgT120I	GGNCC	5 cut(s)	20, 114, 344, 1014, 1969
BmiI	GGNNCC	6 cut(s)	21, 115, 525, 1132, 1709, 1984
BmrFI	CCNGG	3 cut(s)	1490, 1587, 1973
BmsI	GCATC	6 cut(s)	347, 362, 987, 1268, 1413, 1414
BpiI	GAAGAC	1 cut(s)	1048
BplI	GAGNNNNNCTC	2 cut(s)	1290, 1322
BpmI	CTGGAG	2 cut(s)	854, 1922
Bpu10I	CCTNAGC	2 cut(s)	56, 655
Bpu1102I	GCTNAGC	1 cut(s)	474
BpuEI	CTTGAG	1 cut(s)	1270
BsaHI	GRCGYC	1 cut(s)	1583
BsaI	GGTCTC	1 cut(s)	1873
BsaJI	CCNNGG	4 cut(s)	31, 1021, 1354, 1887
BsaWI	WCCGGW	1 cut(s)	1851
BsaXI	ACNNNNNCTCC	6 cut(s)	226, 256, 931, 961, 1038, 1068
Bsc4I	CCNNNNNNNGG	6 cut(s)	109, 110, 111, 586, 1023, 1790
Bse118I	RCCGGY	1 cut(s)	1710
Bse1I	ACTGG	4 cut(s)	278, 527, 1612, 1905
Bse3DI	GCAATG	2 cut(s)	382, 1453
BseBI	CCWGG	3 cut(s)	1490, 1587, 1973
BseDI	CCNNGG	4 cut(s)	31, 1021, 1354, 1887
BseGI	GGATG	6 cut(s)	377, 625, 978, 1283, 1498, 1679
BseLI	CCNNNNNNNGG	6 cut(s)	109, 110, 111, 586, 1023, 1790
BseMI	GCAATG	2 cut(s)	382, 1453
BseMII	CTCAG	5 cut(s)	70, 669, 681, 1396, 2049
BseNI	ACTGG	4 cut(s)	278, 527, 1612, 1905
BseRI	GAGGAG	3 cut(s)	44, 936, 1295
BseSI	GKGCMC	2 cut(s)	511, 1942
BseXI	GCAGC	5 cut(s)	392, 529, 551, 902, 1873
BseYI	CCCAGC	1 cut(s)	383
BsgI	GTGCAG	2 cut(s)	635, 909
BshFI	GGCC	5 cut(s)	345, 500, 1390, 1847, 1998
BshNI	GGYRCC	2 cut(s)	1130, 1707
BsiHKAI	GWGCWC	2 cut(s)	511, 1942
BsiHKCI	CYCGRG	1 cut(s)	1601
BsiSI	CCGG	2 cut(s)	1711, 1852
BslFI	GGGAC	3 cut(s)	33, 158, 1594
BslI	CCNNNNNNNGG	6 cut(s)	109, 110, 111, 586, 1023, 1790
BsmAI	GTCTC	4 cut(s)	965, 1250, 1409, 1873
BsmBI	CGTCTC	1 cut(s)	1250
BsmFI	GGGAC	3 cut(s)	33, 158, 1594
BsmI	GAATGC	1 cut(s)	431
BsnI	GGCC	5 cut(s)	345, 500, 1390, 1847, 1998
Bso31I	GGTCTC	1 cut(s)	1873
BsoBI	CYCGRG	1 cut(s)	1601
Bsp1286I	GDGCHC	3 cut(s)	511, 656, 1942
Bsp143I	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
Bsp1720I	GCTNAGC	1 cut(s)	474
Bsp19I	CCATGG	2 cut(s)	1354, 1887
BspACI	CCGC	5 cut(s)	75, 536, 587, 942, 2021
BspANI	GGCC	5 cut(s)	345, 500, 1390, 1847, 1998
BspCNI	CTCAG	5 cut(s)	69, 668, 682, 1395, 2048
BspLI	GGNNCC	6 cut(s)	21, 115, 525, 1132, 1709, 1984
BspMI	ACCTGC	1 cut(s)	80
BspPI	GGATC	1 cut(s)	1592
BspT107I	GGYRCC	2 cut(s)	1130, 1707
BspTNI	GGTCTC	1 cut(s)	1873
BsrBI	CCGCTC	2 cut(s)	944, 2023
BsrDI	GCAATG	2 cut(s)	382, 1453
BsrFI	RCCGGY	1 cut(s)	1710
BsrI	ACTGG	4 cut(s)	278, 527, 1612, 1905
BssAI	RCCGGY	1 cut(s)	1710
BssECI	CCNNGG	4 cut(s)	31, 1021, 1354, 1887
BssMI	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
BssNI	GRCGYC	1 cut(s)	1583
BssT1I	CCWWGG	4 cut(s)	31, 1021, 1354, 1887
Bst2UI	CCWGG	3 cut(s)	1490, 1587, 1973
BstACI	GRCGYC	1 cut(s)	1583
BstC8I	GCNNGC	3 cut(s)	727, 1033, 1388
BstDEI	CTNAG	6 cut(s)	56, 474, 655, 690, 1382, 2035
BstDSI	CCRYGG	2 cut(s)	1354, 1887
BstF5I	GGATG	6 cut(s)	377, 625, 978, 1283, 1498, 1679
BstH2I	RGCGCY	1 cut(s)	1183
BstHHI	GCGC	1 cut(s)	1182
BstKTI	GATC	6 cut(s)	183, 1236, 1552, 1600, 1668, 1933
BstMAI	GTCTC	4 cut(s)	965, 1250, 1409, 1873
BstMBI	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
BstNI	CCWGG	3 cut(s)	1490, 1587, 1973
BstNSI	RCATGY	2 cut(s)	162, 735
BstSCI	CCNGG	3 cut(s)	1488, 1585, 1971
BstSLI	GKGCMC	2 cut(s)	511, 1942
BstV1I	GCAGC	5 cut(s)	392, 529, 551, 902, 1873
BstV2I	GAAGAC	1 cut(s)	1048
BstX2I	RGATCY	2 cut(s)	1233, 1930
BstXI	CCANNNNNNTGG	1 cut(s)	1355
BstYI	RGATCY	2 cut(s)	1233, 1930
BsuRI	GGCC	5 cut(s)	345, 500, 1390, 1847, 1998
BtgI	CCRYGG	2 cut(s)	1354, 1887
BtgZI	GCGATG	2 cut(s)	1390, 1427
BtrI	CACGTC	1 cut(s)	1244
BtsCI	GGATG	6 cut(s)	377, 625, 978, 1283, 1498, 1679
BtsI	GCAGTG	1 cut(s)	1971
BtsIMutI	CAGTG	8 cut(s)	168, 444, 534, 1344, 1619, 1626, 1971, 2043
BveI	ACCTGC	1 cut(s)	80
Cac8I	GCNNGC	3 cut(s)	727, 1033, 1388
CaiI	CAGNNNCTG	3 cut(s)	533, 545, 1343
CfoI	GCGC	1 cut(s)	1182
Cfr10I	RCCGGY	1 cut(s)	1710
Cfr13I	GGNCC	5 cut(s)	20, 114, 344, 1014, 1969
CseI	GACGC	2 cut(s)	70, 1591
Csp6I	GTAC	2 cut(s)	435, 1898
CviQI	GTAC	2 cut(s)	435, 1898
DdeI	CTNAG	6 cut(s)	56, 474, 655, 690, 1382, 2035
DpnI	GATC	6 cut(s)	182, 1235, 1551, 1599, 1667, 1932
DpnII	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
DraI	TTTAAA	1 cut(s)	2062
DrdI	GACNNNNNNGTC	2 cut(s)	1561, 2039
DseDI	GACNNNNNNGTC	2 cut(s)	1561, 2039
Eco130I	CCWWGG	4 cut(s)	31, 1021, 1354, 1887
Eco24I	GRGCYC	1 cut(s)	656
Eco31I	GGTCTC	1 cut(s)	1873
Eco47I	GGWCC	4 cut(s)	20, 114, 1014, 1969
Eco57I	CTGAAG	4 cut(s)	567, 705, 729, 1042
Eco88I	CYCGRG	1 cut(s)	1601
EcoO109I	RGGNCCY	1 cut(s)	20
EcoRI	GAATTC	1 cut(s)	207
EcoRII	CCWGG	3 cut(s)	1488, 1585, 1971
EcoT14I	CCWWGG	4 cut(s)	31, 1021, 1354, 1887
EcoT22I	ATGCAT	1 cut(s)	340
EcoT38I	GRGCYC	1 cut(s)	656
ErhI	CCWWGG	4 cut(s)	31, 1021, 1354, 1887
Esp3I	CGTCTC	1 cut(s)	1250
FalI	AAGNNNNNCTT	2 cut(s)	1983, 2015
FaqI	GGGAC	3 cut(s)	33, 158, 1594
FauI	CCCGC	2 cut(s)	68, 2028
FbaI	TGATCA	2 cut(s)	180, 1549
FblI	GTMKAC	1 cut(s)	788
Fnu4HI	GCNGC	6 cut(s)	381, 537, 540, 543, 891, 1862
FokI	GGATG	6 cut(s)	384, 632, 965, 1290, 1505, 1666
FriOI	GRGCYC	1 cut(s)	656
Fsp4HI	GCNGC	6 cut(s)	381, 537, 540, 543, 891, 1862
FspBI	CTAG	3 cut(s)	32, 411, 957
GlaI	GCGC	1 cut(s)	1181
GluI	GCNGC	6 cut(s)	381, 537, 540, 543, 891, 1862
GsaI	CCCAGC	1 cut(s)	387
GsuI	CTGGAG	2 cut(s)	854, 1922
HaeII	RGCGCY	1 cut(s)	1183
HaeIII	GGCC	5 cut(s)	345, 500, 1390, 1847, 1998
HapII	CCGG	2 cut(s)	1711, 1852
HgaI	GACGC	2 cut(s)	70, 1591
HhaI	GCGC	1 cut(s)	1182
Hin1I	GRCGYC	1 cut(s)	1583
Hin6I	GCGC	1 cut(s)	1180
HinP1I	GCGC	1 cut(s)	1180
HincII	GTYRAC	2 cut(s)	1566, 1780
HindII	GTYRAC	2 cut(s)	1566, 1780
HindIII	AAGCTT	1 cut(s)	476
HinfI	GANTC	6 cut(s)	463, 849, 913, 1258, 1370, 2033
HpaII	CCGG	2 cut(s)	1711, 1852
HphI	GGTGA	4 cut(s)	175, 991, 1127, 2019
Hpy166II	GTNNAC	7 cut(s)	509, 789, 1138, 1566, 1696, 1780, 1940
Hpy188I	TCNGA	6 cut(s)	206, 246, 294, 394, 1018, 1554
Hpy188III	TCNNGA	8 cut(s)	175, 212, 580, 657, 1247, 1287, 1601, 1663
Hpy8I	GTNNAC	7 cut(s)	509, 789, 1138, 1566, 1696, 1780, 1940
HpyAV	CCTTC	5 cut(s)	993, 1002, 1006, 1448, 1858
HpyCH4IV	ACGT	4 cut(s)	354, 886, 1243, 1291
HpyF3I	CTNAG	6 cut(s)	56, 474, 655, 690, 1382, 2035
HpySE526I	ACGT	4 cut(s)	354, 886, 1243, 1291
Hsp92I	GRCGYC	1 cut(s)	1583
HspAI	GCGC	1 cut(s)	1180
Ksp22I	TGATCA	2 cut(s)	180, 1549
Kzo9I	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
LmnI	GCTCC	5 cut(s)	78, 949, 1210, 1576, 1982
Lsp1109I	GCAGC	5 cut(s)	392, 529, 551, 902, 1873
LweI	GCATC	6 cut(s)	347, 362, 987, 1268, 1413, 1414
MaeI	CTAG	3 cut(s)	32, 411, 957
MaeII	ACGT	4 cut(s)	354, 886, 1243, 1291
MaeIII	GTNAC	6 cut(s)	154, 250, 399, 439, 1530, 2041
MalI	GATC	6 cut(s)	182, 1235, 1551, 1599, 1667, 1932
MbiI	CCGCTC	2 cut(s)	944, 2023
MboI	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
MboII	GAAGA	6 cut(s)	31, 202, 892, 1048, 1904, 1940
MflI	RGATCY	2 cut(s)	1233, 1930
MhlI	GDGCHC	3 cut(s)	511, 656, 1942
MlyI	GAGTC	1 cut(s)	2027
MmeI	TCCRAC	5 cut(s)	141, 269, 512, 796, 1041
Mph1103I	ATGCAT	1 cut(s)	340
MseI	TTAA	2 cut(s)	1238, 2061
MslI	CAYNNNNRTG	4 cut(s)	1353, 1446, 1464, 1713
MspA1I	CMGCKG	5 cut(s)	383, 542, 1386, 1421, 1832
MspI	CCGG	2 cut(s)	1711, 1852
MspR9I	CCNGG	3 cut(s)	1490, 1587, 1973
Mva1269I	GAATGC	1 cut(s)	431
MvaI	CCWGG	3 cut(s)	1490, 1587, 1973
NcoI	CCATGG	2 cut(s)	1354, 1887
NdeII	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
NlaIV	GGNNCC	6 cut(s)	21, 115, 525, 1132, 1709, 1984
NmeAIII	GCCGAG	1 cut(s)	1322
NmuCI	GTSAC	6 cut(s)	154, 250, 399, 439, 1530, 2041
NsiI	ATGCAT	1 cut(s)	340
NspI	RCATGY	2 cut(s)	162, 735
PciI	ACATGT	1 cut(s)	731
PctI	GAATGC	1 cut(s)	431
PfeI	GAWTC	5 cut(s)	463, 849, 913, 1258, 1370
PfoI	TCCNGGA	2 cut(s)	1488, 1971
PkrI	GCNGC	6 cut(s)	382, 538, 541, 544, 892, 1863
PleI	GAGTC	1 cut(s)	2027
PpsI	GAGTC	1 cut(s)	2027
PpuMI	RGGWCCY	1 cut(s)	20
PscI	ACATGT	1 cut(s)	731
Psp5II	RGGWCCY	1 cut(s)	20
Psp6I	CCWGG	3 cut(s)	1488, 1585, 1971
PspFI	CCCAGC	1 cut(s)	383
PspGI	CCWGG	3 cut(s)	1488, 1585, 1971
PspN4I	GGNNCC	6 cut(s)	21, 115, 525, 1132, 1709, 1984
PspPI	GGNCC	5 cut(s)	20, 114, 344, 1014, 1969
PspPPI	RGGWCCY	1 cut(s)	20
PstNI	CAGNNNCTG	3 cut(s)	533, 545, 1343
PsuI	RGATCY	2 cut(s)	1233, 1930
PvuII	CAGCTG	5 cut(s)	383, 542, 1386, 1421, 1832
RsaI	GTAC	2 cut(s)	436, 1899
RsaNI	GTAC	2 cut(s)	435, 1898
RseI	CAYNNNNRTG	4 cut(s)	1353, 1446, 1464, 1713
SaqAI	TTAA	2 cut(s)	1238, 2061
SatI	GCNGC	6 cut(s)	381, 537, 540, 543, 891, 1862
Sau3AI	GATC	6 cut(s)	180, 1233, 1549, 1597, 1665, 1930
Sau96I	GGNCC	5 cut(s)	20, 114, 344, 1014, 1969
ScaI	AGTACT	1 cut(s)	1899
SchI	GAGTC	1 cut(s)	2027
ScrFI	CCNGG	3 cut(s)	1490, 1587, 1973
SduI	GDGCHC	3 cut(s)	511, 656, 1942
SfaNI	GCATC	6 cut(s)	347, 362, 987, 1268, 1413, 1414
SinI	GGWCC	4 cut(s)	20, 114, 1014, 1969
SmiMI	CAYNNNNRTG	4 cut(s)	1353, 1446, 1464, 1713
SmlI	CTYRAG	1 cut(s)	1285
SmoI	CTYRAG	1 cut(s)	1285
SpeI	ACTAGT	2 cut(s)	410, 956
SsiI	CCGC	5 cut(s)	75, 536, 587, 942, 2021
SspMI	CTAG	3 cut(s)	32, 411, 957
StyD4I	CCNGG	3 cut(s)	1488, 1585, 1971
StyI	CCWWGG	4 cut(s)	31, 1021, 1354, 1887
TaiI	ACGT	4 cut(s)	357, 889, 1246, 1294
TaqI	TCGA	3 cut(s)	953, 1248, 1668
TatI	WGTACW	2 cut(s)	434, 1897
TauI	GCSGC	1 cut(s)	539
TfiI	GAWTC	5 cut(s)	463, 849, 913, 1258, 1370
Tru1I	TTAA	2 cut(s)	1238, 2061
Tru9I	TTAA	2 cut(s)	1238, 2061
TscAI	CASTG	8 cut(s)	175, 444, 534, 1344, 1619, 1633, 1971, 2050
TseFI	GTSAC	6 cut(s)	154, 250, 399, 439, 1530, 2041
TseI	GCWGC	5 cut(s)	380, 539, 542, 890, 1861
Tsp45I	GTSAC	6 cut(s)	154, 250, 399, 439, 1530, 2041
TspGWI	ACGGA	2 cut(s)	384, 1347
TspRI	CASTG	8 cut(s)	175, 444, 534, 1344, 1619, 1633, 1971, 2050
VneI	GTGCAC	2 cut(s)	507, 1938
VpaK11BI	GGWCC	4 cut(s)	20, 114, 1014, 1969
XapI	RAATTY	7 cut(s)	207, 757, 1090, 1499, 1723, 1749, 2008
XceI	RCATGY	2 cut(s)	162, 735
XmaJI	CCTAGG	1 cut(s)	31
XmiI	GTMKAC	1 cut(s)	788
XspI	CTAG	3 cut(s)	32, 411, 957
ZrmI	AGTACT	1 cut(s)	1899
Zsp2I	ATGCAT	1 cut(s)	340

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.