Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004888

Physical Location & Seq

Genomic Coordinates Reverse (-)

34944650 .. 34952091

Gene Structure 7442 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013645

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGAAGAATATCAAGTTCAGAATTCTTGACATGAGAAATAGTAGTACTACAACAAGTCCTACCGTAAAAGTTGCTAGGCAGGATTACTGGCAAGATATCTGTCCCTCGACATATGTTTCCACAAACCTCGACTTCTCTCTCTTCGCTTACTCTTCTGGGCTTGCGAACGTGTCTTTTTGGTACGGATGCAATGCAACCGTAACAGTCCCAGTTGATCCAGTAACTACGGGCGCCTGTCAATATAAGGTTTTGGTTCCGGTATTTGAATCCGCTTCTGCGGCTCTGGACAGCAATACTACAGATATTGAGACTGCGATAGATGGTGGTTTTGAATTGGATGTGCTAAATGATGATACTGGCAAGTGCAACAATTGCTTGGCATCAGGGGGAGTTTGTGGGCAAAACACTGCTGCTGCAAACGAATTCATGTGCTTTTGCCAGACGAATTCATCTTCGACAACCATATGTACTGAAAACTCTGCATCAACTCCAACTTCAGCTCCATCCGGTTCAGAAATGCATCCCTATCTTCTCCTCCTGCACCTCTTAATAATAATACTAGTATTCTTATCTGGGGTACCAAAATCCTTGGGGGTTGATGATGAGCAGTACCTCAACTGCAGCCAATCGTTTGATTGTGCAAGTATCGGGAGCATCGGTTACCCTTTCTGGGGATCAAACCGGCCCGACTACTGTGGCTTCCCGGAGTTCGAGTTGAACTGCAGCGGCGACGCCCCGTCCATCTCAATCCAGAAACAAGAATTTCGAGTCCTTTCTATCAATCCGAGTCAAAGCAGTTTCACAATTGCTAGAGCCGATTACTGGAACACCGTTTGTCCGGTGTCCCTTACCAATACCACCACCATAGATAATTCATCATCCCGTGTTCTTAAATTTGCTTCGGATGTTCAACAACTCTGGATCTTCTATGCATGCCCCTCACTTAATATTCCTCTCCCACCTCAGCTTACCAGCCAATTCAGCTGCCCCATAAACTCTACTACGGATTCCATCAACTACTATTTGACGAAAGATCTTGATCTTGACATTTTCGGGCTATCCAACATCAGTAGCACCTTCGGAACTTGCAACACAACTATCAGCGTCCGAGTTTCCCAATCGGCAGCTTCCAATTTAGCATTGAATACGTCAAAGGAAAATCTAGGCTTGGCGCTTGATTCCGGGTTTAATTTGACATGGGATGCAAGCAATACCTTGTGCAAACAATGTCAAGACTCTGGAGGTCAATGCGGCTACAATACATCTTCATCTGAATTCACCTGTTATTGCAAGGATGGACCTAATCTCTCTGATTGTAGAGGAAAGAATAGTCAAGTACCAATAGGTCTTGCTGTAGGAGGTGCAATCCTTTTTGGCATTTTTGTGGGATTCTACACATATTTTGTCATCCAGCGAAAGAAGAAGAAACGTGCAGCAGAAGCTCAGAGCAAAGAGATTCCCACCCCACTTACAAGCAAAAGCACTGTTACTCCTTCGACTAATCTTTCTCAATCTCAAAGCATCCCTTCTTATCCTTCCTTTAACTCAAAATCAAGGTCTGACCTTGGAAAAGCCAGCACTTACTTTGGAGTTCAGGTCTTCAGCTATGCTGAATTAGAGGAAGCCACTGAAAATTTCAACCCTGCTAAAGAACTTGGAGATGGAGGCTTTGGCACCGTTTACTATGGTAAGCTACATGATGGCCGTGTAGTTGCAGTGAAGCGCCTTTATGAAAACAATTTCAAACGGGTGGAGCAGTTTATGAATGAGGTTGAAATCTTAACACGTCTGGAACACCAGCATCTTGTGAAACTCTATGGATGCACCTCAAGACGTAGCCGAGAACTCCTTCTTGTCTATGAATATATTTCTAATGGAACAGTGGCTGACCATCTCCATGGAAAACGAATGGAGTCTGGCTTTCTCAGTTGGCCTGTTAGATTGAGCATCGCCATAGAGACAGCTGATGCACTGGCTTTCCTTCATCGCAATGATGTGATACACCGTGATGTCAAAACCAACAATATACTTCTAACTGACGACTTTTGTGTGAAAGTTGCTGATTTTGGTCTGTCACGGTTGTTCCCCAATGATCAGACCCATGTGTCAACTGCTCCACAAGGGACGCCGGGCTATGTTGATCCCGAGTATTACCAGTGCTATCAACTCACTGACAAAAGTGATGTTTATAGCTTTGGGGTGGTTTTGATCGAGCTTATATCATCCTTACAAGCTGTCGACACCAACAGGCACCGGCATGATATAAATTTGGCCAACATGGCTATCAACAAAATTCAAAATCATTTGGTGAATGAGTTGGTTGATCCACTTCTTGGGTATGACAAGGACTATTCTGTGAGGATAATGGCAACAGCTGTTGCAGAATTGGCCTTCCGGTGTTTGCAGCAGGAGAAAGATATGAGACCAACCATGGAAGAAGTACTGGAGGGTTTAAAAGCAATCCAAAATGAAGACCTTGGGGCACAAGAAAATGGGGAGGCAGAGGCAGTGGTCCTGGATATAGGAGCTGATGAAGTTGGGCTTCTGAGGAATTTGCCTCCTCCACTCTCACCAGACTCACTTGTCACTGATAAATTGATTTTAAAAAGGTGGGACAGAACTATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

876

Amino Acids

96.27

Weight (kDa)

4.98

Isoelectric Point (pI)

40.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WAK_assoc	PF14380	76 - 147	3.1e-12	Wall-associated receptor kinase C-terminal
GUB_WAK_bind	PF13947	207 - 271	9.5e-15	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	357 - 433	1e-19	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	548 - 816	6.9e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	549 - 817	1.8e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004888)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66880 AT1G66880 AT1G66880 AT5G38210 AT5G38210 AT5G38210
fragaria_vesca	FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540 FvH4_2g12540
malus_domestica	MD00G1204100.v1.1 MD09G1255300.v1.1 MD17G1247800.v1.1 MD17G1248000.v1.1
prunus_persica	Prupe.3G146500_v2.0.a1 Prupe.3G146700_v2.0.a1
pyrus_communis	pycom09g17110 pycom17g24910 pycom17g25040
rosa_chinensis	RchiOBHm_Chr6g0273041
rosa_laevigata	RLG00000013645
rosa_multiflora	Rmu_ssc0000387.1_g000003
rosa_roxburghii	Rroxscaffold_7G00195700
rosa_rugosa	Rorug06G0072500
rosa_samantha	Rh6AG188100 Rh6BG191500 Rh6CG190600 Rh6DG182000
rosa_wichuraiana	Rw6G016310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	2104, 2582
Acc65I	GGTACC	1 cut(s)	577
AccB1I	GGYRCC	4 cut(s)	230, 577, 1673, 2250
AccB7I	CCANNNNNTGG	1 cut(s)	2333
AccI	GTMKAC	1 cut(s)	2238
AciI	CCGC	4 cut(s)	270, 278, 726, 1251
AclWI	GGATC	5 cut(s)	209, 682, 929, 2135, 2318
AcoI	YGGCCR	2 cut(s)	1702, 2271
AcuI	CTGAAG	2 cut(s)	480, 1585
AcyI	GRCGYC	3 cut(s)	231, 732, 2126
AfaI	GTAC	7 cut(s)	46, 182, 469, 579, 611, 1338, 2442
AfiI	CCNNNNNNNGG	4 cut(s)	670, 671, 822, 2333
AflIII	ACRYGT	2 cut(s)	168, 1784
AgsI	TTSAA	9 cut(s)	266, 332, 718, 911, 1144, 1639, 1744, 1775, 2297
AhdI	GACNNNNNGTC	1 cut(s)	736
AhlI	ACTAGT	1 cut(s)	559
AjiI	CACGTC	1 cut(s)	1787
AjnI	CCWGG	1 cut(s)	2514
Alw26I	GTCTC	3 cut(s)	302, 1952, 2416
AlwI	GGATC	5 cut(s)	209, 682, 929, 2135, 2318
AlwNI	CAGNNNCTG	1 cut(s)	1886
Ama87I	CYCGRG	1 cut(s)	2144
AoxI	GGCC	5 cut(s)	683, 1702, 1931, 2271, 2388
ApeKI	GCWGC	8 cut(s)	410, 413, 621, 723, 984, 1124, 1433, 2404
ArsI	GACNNNNNNTTYG	2 cut(s)	1541, 1573
Asp700I	GAANNNNTTC	1 cut(s)	1848
Asp718I	GGTACC	1 cut(s)	577
AspLEI	GCGC	3 cut(s)	233, 1174, 1725
AspS9I	GGNCC	3 cut(s)	684, 1298, 2512
AsuC2I	CCSGG	3 cut(s)	704, 1183, 2130
AsuHPI	GGTGA	3 cut(s)	1270, 2320, 2562
AvaI	CYCGRG	1 cut(s)	2144
AvaII	GGWCC	2 cut(s)	1298, 2512
BaeGI	GKGCMC	1 cut(s)	2485
BalI	TGGCCA	1 cut(s)	2273
BanI	GGYRCC	4 cut(s)	230, 577, 1673, 2250
BbsI	GAAGAC	2 cut(s)	1591, 2478
BbvCI	CCTCAGC	1 cut(s)	963
BbvI	GCAGC	8 cut(s)	397, 400, 633, 735, 971, 1136, 1445, 2416
BccI	CCATC	8 cut(s)	314, 511, 749, 1019, 1289, 1655, 1694, 1899
BceAI	ACGGC	1 cut(s)	1689
BciT130I	CCWGG	1 cut(s)	2516
BclI	TGATCA	1 cut(s)	2092
BcnI	CCSGG	3 cut(s)	704, 1183, 2130
BcoDI	GTCTC	3 cut(s)	302, 1952, 2416
BcuI	ACTAGT	1 cut(s)	559
BfaI	CTAG	4 cut(s)	75, 560, 810, 1163
BfmI	CTRYAG	4 cut(s)	297, 619, 721, 1353
BfoI	RGCGCY	3 cut(s)	234, 1175, 1726
BglI	GCCNNNNNGGC	1 cut(s)	2279
BglII	AGATCT	1 cut(s)	1033
BmcAI	AGTACT	2 cut(s)	46, 2442
Bme1390I	CCNGG	4 cut(s)	704, 1183, 2130, 2516
Bme18I	GGWCC	2 cut(s)	1298, 2512
BmeRI	GACNNNNNGTC	1 cut(s)	736
BmeT110I	CYCGRG	1 cut(s)	2144
BmgBI	CACGTC	1 cut(s)	1787
BmgT120I	GGNCC	3 cut(s)	684, 1298, 2512
BmiI	GGNNCC	5 cut(s)	232, 255, 579, 1675, 2252
BmrFI	CCNGG	4 cut(s)	704, 1183, 2130, 2516
BmrI	ACTGGG	1 cut(s)	203
BmuI	ACTGGG	1 cut(s)	203
BpiI	GAAGAC	2 cut(s)	1591, 2478
BpmI	CTGGAG	2 cut(s)	1260, 2465
Bpu10I	CCTNAGC	1 cut(s)	963
BpuEI	CTTGAG	1 cut(s)	1813
BpuMI	CCSGG	3 cut(s)	704, 1183, 2130
BsaBI	GATNNNNATC	1 cut(s)	1038
BsaHI	GRCGYC	3 cut(s)	231, 732, 2126
BsaI	GGTCTC	1 cut(s)	2416
BsaJI	CCNNGG	5 cut(s)	588, 1564, 1897, 2430, 2476
BsaWI	WCCGGW	4 cut(s)	256, 506, 838, 2394
Bsc4I	CCNNNNNNNGG	4 cut(s)	670, 671, 822, 2333
Bse118I	RCCGGY	2 cut(s)	681, 2253
Bse1I	ACTGG	8 cut(s)	92, 209, 218, 361, 827, 1977, 2155, 2448
Bse3DI	GCAATG	2 cut(s)	196, 1996
Bse8I	GATNNNNATC	1 cut(s)	1038
BseBI	CCWGG	1 cut(s)	2516
BseDI	CCNNGG	5 cut(s)	588, 1564, 1897, 2430, 2476
BseJI	GATNNNNATC	1 cut(s)	1038
BseLI	CCNNNNNNNGG	4 cut(s)	670, 671, 822, 2333
BseMI	GCAATG	2 cut(s)	196, 1996
BseMII	CTCAG	4 cut(s)	977, 1457, 1939, 2537
BseNI	ACTGG	8 cut(s)	92, 209, 218, 361, 827, 1977, 2155, 2448
BseRI	GAGGAG	2 cut(s)	524, 2550
BseSI	GKGCMC	1 cut(s)	2485
BseXI	GCAGC	8 cut(s)	397, 400, 633, 735, 971, 1136, 1445, 2416
BsgI	GTGCAG	2 cut(s)	524, 1452
BshFI	GGCC	5 cut(s)	685, 1704, 1933, 2273, 2390
BshNI	GGYRCC	4 cut(s)	230, 577, 1673, 2250
BsiHKCI	CYCGRG	1 cut(s)	2144
BsiSI	CCGG	9 cut(s)	257, 507, 682, 704, 839, 1182, 2129, 2254, 2395
BslFI	GGGAC	4 cut(s)	87, 191, 829, 2137
BslI	CCNNNNNNNGG	4 cut(s)	670, 671, 822, 2333
BsmAI	GTCTC	3 cut(s)	302, 1952, 2416
BsmFI	GGGAC	4 cut(s)	87, 191, 829, 2137
BsnI	GGCC	5 cut(s)	685, 1704, 1933, 2273, 2390
Bso31I	GGTCTC	1 cut(s)	2416
BsoBI	CYCGRG	1 cut(s)	2144
Bsp1286I	GDGCHC	1 cut(s)	2485
Bsp143I	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
Bsp19I	CCATGG	2 cut(s)	1897, 2430
BspACI	CCGC	4 cut(s)	270, 278, 726, 1251
BspANI	GGCC	5 cut(s)	685, 1704, 1933, 2273, 2390
BspCNI	CTCAG	4 cut(s)	976, 1456, 1938, 2538
BspLI	GGNNCC	5 cut(s)	232, 255, 579, 1675, 2252
BspMAI	CTGCAG	2 cut(s)	623, 725
BspPI	GGATC	5 cut(s)	209, 682, 929, 2135, 2318
BspT107I	GGYRCC	4 cut(s)	230, 577, 1673, 2250
BspTNI	GGTCTC	1 cut(s)	2416
BsrDI	GCAATG	2 cut(s)	196, 1996
BsrFI	RCCGGY	2 cut(s)	681, 2253
BsrI	ACTGG	8 cut(s)	92, 209, 218, 361, 827, 1977, 2155, 2448
BssAI	RCCGGY	2 cut(s)	681, 2253
BssECI	CCNNGG	5 cut(s)	588, 1564, 1897, 2430, 2476
BssMI	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
BssNI	GRCGYC	3 cut(s)	231, 732, 2126
BssT1I	CCWWGG	5 cut(s)	588, 1564, 1897, 2430, 2476
Bst2UI	CCWGG	1 cut(s)	2516
Bst6I	CTCTTC	2 cut(s)	146, 157
BstACI	GRCGYC	3 cut(s)	231, 732, 2126
BstAPI	GCANNNNNTGC	1 cut(s)	372
BstC8I	GCNNGC	4 cut(s)	162, 934, 1207, 1576
BstDEI	CTNAG	4 cut(s)	963, 1443, 1925, 2546
BstDSI	CCRYGG	2 cut(s)	1897, 2430
BstEII	GGTNACC	1 cut(s)	659
BstH2I	RGCGCY	3 cut(s)	234, 1175, 1726
BstHHI	GCGC	3 cut(s)	233, 1174, 1725
BstKTI	GATC	9 cut(s)	217, 677, 924, 1036, 1042, 2095, 2143, 2211, 2326
BstMAI	GTCTC	3 cut(s)	302, 1952, 2416
BstMBI	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
BstMWI	GCNNNNNNNGC	6 cut(s)	278, 372, 981, 1439, 2279, 2387
BstNI	CCWGG	1 cut(s)	2516
BstNSI	RCATGY	1 cut(s)	936
BstPI	GGTNACC	1 cut(s)	659
BstSCI	CCNGG	4 cut(s)	702, 1181, 2128, 2514
BstSFI	CTRYAG	4 cut(s)	297, 619, 721, 1353
BstSLI	GKGCMC	1 cut(s)	2485
BstV1I	GCAGC	8 cut(s)	397, 400, 633, 735, 971, 1136, 1445, 2416
BstV2I	GAAGAC	2 cut(s)	1591, 2478
BstX2I	RGATCY	2 cut(s)	921, 1033
BstXI	CCANNNNNNTGG	1 cut(s)	1898
BstYI	RGATCY	2 cut(s)	921, 1033
BsuRI	GGCC	5 cut(s)	685, 1704, 1933, 2273, 2390
BtgI	CCRYGG	2 cut(s)	1897, 2430
BtgZI	GCGATG	2 cut(s)	1933, 1970
BtrI	CACGTC	1 cut(s)	1787
BtsI	GCAGTG	3 cut(s)	405, 1722, 2514
Cac8I	GCNNGC	4 cut(s)	162, 934, 1207, 1576
CaiI	CAGNNNCTG	1 cut(s)	1886
CfoI	GCGC	3 cut(s)	233, 1174, 1725
Cfr10I	RCCGGY	2 cut(s)	681, 2253
Cfr13I	GGNCC	3 cut(s)	684, 1298, 2512
CseI	GACGC	3 cut(s)	740, 1093, 2134
Csp6I	GTAC	7 cut(s)	45, 181, 468, 578, 610, 1337, 2441
CviQI	GTAC	7 cut(s)	45, 181, 468, 578, 610, 1337, 2441
DdeI	CTNAG	4 cut(s)	963, 1443, 1925, 2546
DinI	GGCGCC	1 cut(s)	232
DpnI	GATC	9 cut(s)	216, 676, 923, 1035, 1041, 2094, 2142, 2210, 2325
DpnII	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
DraI	TTTAAA	2 cut(s)	2454, 2604
DrdI	GACNNNNNNGTC	2 cut(s)	2104, 2582
DriI	GACNNNNNGTC	1 cut(s)	736
DseDI	GACNNNNNNGTC	2 cut(s)	2104, 2582
EaeI	YGGCCR	2 cut(s)	1702, 2271
Eam1104I	CTCTTC	2 cut(s)	146, 157
Eam1105I	GACNNNNNGTC	1 cut(s)	736
EarI	CTCTTC	2 cut(s)	146, 157
Eco130I	CCWWGG	5 cut(s)	588, 1564, 1897, 2430, 2476
Eco31I	GGTCTC	1 cut(s)	2416
Eco32I	GATATC	1 cut(s)	97
Eco47I	GGWCC	2 cut(s)	1298, 2512
Eco57I	CTGAAG	2 cut(s)	480, 1585
Eco88I	CYCGRG	1 cut(s)	2144
Eco91I	GGTNACC	1 cut(s)	659
EcoO65I	GGTNACC	1 cut(s)	659
EcoRI	GAATTC	4 cut(s)	21, 422, 445, 1274
EcoRII	CCWGG	1 cut(s)	2514
EcoRV	GATATC	1 cut(s)	97
EcoT14I	CCWWGG	5 cut(s)	588, 1564, 1897, 2430, 2476
EcoT22I	ATGCAT	2 cut(s)	522, 934
EgeI	GGCGCC	1 cut(s)	232
EheI	GGCGCC	1 cut(s)	232
ErhI	CCWWGG	5 cut(s)	588, 1564, 1897, 2430, 2476
FalI	AAGNNNNNCTT	4 cut(s)	1510, 1542, 2526, 2558
FaqI	GGGAC	4 cut(s)	87, 191, 829, 2137
FauNDI	CATATG	2 cut(s)	112, 464
FbaI	TGATCA	1 cut(s)	2092
FblI	GTMKAC	1 cut(s)	2238
FspBI	CTAG	4 cut(s)	75, 560, 810, 1163
GlaI	GCGC	3 cut(s)	232, 1173, 1724
GsuI	CTGGAG	2 cut(s)	1260, 2465
HaeII	RGCGCY	3 cut(s)	234, 1175, 1726
HaeIII	GGCC	5 cut(s)	685, 1704, 1933, 2273, 2390
HapII	CCGG	9 cut(s)	257, 507, 682, 704, 839, 1182, 2129, 2254, 2395
HgaI	GACGC	3 cut(s)	740, 1093, 2134
HhaI	GCGC	3 cut(s)	233, 1174, 1725
Hin1I	GRCGYC	3 cut(s)	231, 732, 2126
Hin6I	GCGC	3 cut(s)	231, 1172, 1723
HinP1I	GCGC	3 cut(s)	231, 1172, 1723
HincII	GTYRAC	2 cut(s)	2109, 2239
HindII	GTYRAC	2 cut(s)	2109, 2239
HpaII	CCGG	9 cut(s)	257, 507, 682, 704, 839, 1182, 2129, 2254, 2395
HphI	GGTGA	3 cut(s)	1270, 2320, 2562
Hpy166II	GTNNAC	3 cut(s)	1681, 2109, 2239
Hpy8I	GTNNAC	3 cut(s)	1681, 2109, 2239
Hpy99I	CGWCG	1 cut(s)	734
HpyAV	CCTTC	7 cut(s)	1087, 1503, 1536, 1545, 1859, 1991, 2401
HpyCH4IV	ACGT	5 cut(s)	168, 1148, 1429, 1786, 1834
HpyF10VI	GCNNNNNNNGC	6 cut(s)	278, 372, 981, 1439, 2279, 2387
HpyF3I	CTNAG	4 cut(s)	963, 1443, 1925, 2546
HpySE526I	ACGT	5 cut(s)	168, 1148, 1429, 1786, 1834
Hsp92I	GRCGYC	3 cut(s)	231, 732, 2126
HspAI	GCGC	3 cut(s)	231, 1172, 1723
KasI	GGCGCC	1 cut(s)	230
KpnI	GGTACC	1 cut(s)	581
Ksp22I	TGATCA	1 cut(s)	2092
Kzo9I	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
LmnI	GCTCC	5 cut(s)	505, 651, 1753, 2119, 2525
Lsp1109I	GCAGC	8 cut(s)	397, 400, 633, 735, 971, 1136, 1445, 2416
MaeI	CTAG	4 cut(s)	75, 560, 810, 1163
MaeII	ACGT	5 cut(s)	168, 1148, 1429, 1786, 1834
MaeIII	GTNAC	6 cut(s)	199, 220, 659, 1486, 2073, 2584
MalI	GATC	9 cut(s)	216, 676, 923, 1035, 1041, 2094, 2142, 2210, 2325
MboI	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
MfeI	CAATTG	2 cut(s)	370, 804
MflI	RGATCY	2 cut(s)	921, 1033
MhlI	GDGCHC	1 cut(s)	2485
MlsI	TGGCCA	1 cut(s)	2273
MluNI	TGGCCA	1 cut(s)	2273
Mly113I	GGCGCC	1 cut(s)	231
MlyI	GAGTC	5 cut(s)	777, 796, 1229, 1922, 2570
MmeI	TCCRAC	2 cut(s)	515, 1086
Mox20I	TGGCCA	1 cut(s)	2273
Mph1103I	ATGCAT	2 cut(s)	522, 934
MroXI	GAANNNNTTC	1 cut(s)	1848
MscI	TGGCCA	1 cut(s)	2273
MseI	TTAA	8 cut(s)	548, 891, 945, 1188, 1542, 1781, 2453, 2603
MslI	CAYNNNNRTG	5 cut(s)	1382, 1896, 1989, 2007, 2256
Msp20I	TGGCCA	1 cut(s)	2273
MspA1I	CMGCKG	4 cut(s)	726, 984, 1964, 2375
MspI	CCGG	9 cut(s)	257, 507, 682, 704, 839, 1182, 2129, 2254, 2395
MspR9I	CCNGG	4 cut(s)	704, 1183, 2130, 2516
MunI	CAATTG	2 cut(s)	370, 804
MvaI	CCWGG	1 cut(s)	2516
MwoI	GCNNNNNNNGC	6 cut(s)	278, 372, 981, 1439, 2279, 2387
NarI	GGCGCC	1 cut(s)	231
NciI	CCSGG	3 cut(s)	704, 1183, 2130
NcoI	CCATGG	2 cut(s)	1897, 2430
NdeI	CATATG	2 cut(s)	112, 464
NdeII	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
NlaIV	GGNNCC	5 cut(s)	232, 255, 579, 1675, 2252
NmeAIII	GCCGAG	1 cut(s)	1865
NmuCI	GTSAC	2 cut(s)	2073, 2584
NsiI	ATGCAT	2 cut(s)	522, 934
NspI	RCATGY	1 cut(s)	936
PaeI	GCATGC	1 cut(s)	936
PdmI	GAANNNNTTC	1 cut(s)	1848
PfeI	GAWTC	5 cut(s)	266, 1007, 1178, 1389, 1456
PflMI	CCANNNNNTGG	1 cut(s)	2333
PfoI	TCCNGGA	2 cut(s)	702, 2514
PleI	GAGTC	5 cut(s)	776, 795, 1229, 1921, 2570
PluTI	GGCGCC	1 cut(s)	234
PpsI	GAGTC	5 cut(s)	776, 795, 1229, 1921, 2570
Psp6I	CCWGG	1 cut(s)	2514
PspEI	GGTNACC	1 cut(s)	659
PspGI	CCWGG	1 cut(s)	2514
PspN4I	GGNNCC	5 cut(s)	232, 255, 579, 1675, 2252
PspPI	GGNCC	3 cut(s)	684, 1298, 2512
PstI	CTGCAG	2 cut(s)	623, 725
PstNI	CAGNNNCTG	1 cut(s)	1886
PsuI	RGATCY	2 cut(s)	921, 1033
PvuII	CAGCTG	3 cut(s)	984, 1964, 2375
RsaI	GTAC	7 cut(s)	46, 182, 469, 579, 611, 1338, 2442
RsaNI	GTAC	7 cut(s)	45, 181, 468, 578, 610, 1337, 2441
RseI	CAYNNNNRTG	5 cut(s)	1382, 1896, 1989, 2007, 2256
SalI	GTCGAC	1 cut(s)	2237
SaqAI	TTAA	8 cut(s)	548, 891, 945, 1188, 1542, 1781, 2453, 2603
Sau3AI	GATC	9 cut(s)	214, 674, 921, 1033, 1039, 2092, 2140, 2208, 2323
Sau96I	GGNCC	3 cut(s)	684, 1298, 2512
ScaI	AGTACT	2 cut(s)	46, 2442
SchI	GAGTC	5 cut(s)	777, 796, 1229, 1922, 2570
ScrFI	CCNGG	4 cut(s)	704, 1183, 2130, 2516
SduI	GDGCHC	1 cut(s)	2485
SfcI	CTRYAG	4 cut(s)	297, 619, 721, 1353
SfoI	GGCGCC	1 cut(s)	232
SinI	GGWCC	2 cut(s)	1298, 2512
SmiMI	CAYNNNNRTG	5 cut(s)	1382, 1896, 1989, 2007, 2256
SmlI	CTYRAG	1 cut(s)	1828
SmoI	CTYRAG	1 cut(s)	1828
SpeI	ACTAGT	1 cut(s)	559
SphI	GCATGC	1 cut(s)	936
SsiI	CCGC	4 cut(s)	270, 278, 726, 1251
SspDI	GGCGCC	1 cut(s)	230
SspI	AATATT	1 cut(s)	949
SspMI	CTAG	4 cut(s)	75, 560, 810, 1163
StyD4I	CCNGG	4 cut(s)	702, 1181, 2128, 2514
StyI	CCWWGG	5 cut(s)	588, 1564, 1897, 2430, 2476
TaiI	ACGT	5 cut(s)	171, 1151, 1432, 1789, 1837
TaqI	TCGA	8 cut(s)	107, 129, 455, 711, 766, 1496, 2211, 2238
TatI	WGTACW	3 cut(s)	44, 467, 2440
TauI	GCSGC	3 cut(s)	281, 729, 1254
TfiI	GAWTC	5 cut(s)	266, 1007, 1178, 1389, 1456
Tru1I	TTAA	8 cut(s)	548, 891, 945, 1188, 1542, 1781, 2453, 2603
Tru9I	TTAA	8 cut(s)	548, 891, 945, 1188, 1542, 1781, 2453, 2603
TseFI	GTSAC	2 cut(s)	2073, 2584
TseI	GCWGC	8 cut(s)	410, 413, 621, 723, 984, 1124, 1433, 2404
Tsp45I	GTSAC	2 cut(s)	2073, 2584
TspGWI	ACGGA	2 cut(s)	198, 1019
Van91I	CCANNNNNTGG	1 cut(s)	2333
VpaK11BI	GGWCC	2 cut(s)	1298, 2512
XceI	RCATGY	1 cut(s)	936
XmiI	GTMKAC	1 cut(s)	2238
XmnI	GAANNNNTTC	1 cut(s)	1848
XspI	CTAG	4 cut(s)	75, 560, 810, 1163
ZrmI	AGTACT	2 cut(s)	46, 2442
Zsp2I	ATGCAT	2 cut(s)	522, 934

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.