AT1G68220

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007471
Physical Location & Seq
Forward (+)
25570092 .. 25571733
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68220.1

Sequence Viewer

Length: 606 bp
ATGGCGGTTTCAGTTTCAATCCTCACCGTCGTCACTGCTCTTCACCTCCTTGCTTTCGTCTTCGCCTTCGGTGCCGAGCGTCGCCGTAGCACCGCTGTGCCGGTTCCGGATCAGTACGATGAGAAGACTATTTGTAAGTACGGAACTGAGGCGTCGACAGTGTACGGTATGTCGGCGTTTGGGTTGCTTCTTGTTAGCCAAGCGGTGGTTAACGGTGTTACTAAGTGTCTCTGTTTTGGAAAGGGTCTTGTTACTGGTACTTCTTACACTGTCTGGGCTATTGTCTTCTTCGTTGTTTCTTGGGTAAGCTTTTTAGGAGCTGAGGCGTGCTTGTTGGGTGGATCAGCGAGGAACGCGTACCACACTAAAAGTGAAGGCATTTACAAAGGGAAAGAGCTTTCGTGTGCCGTCTTACCCGTTGGAGTCTTTGCTGCTGGAGCTGCTTTCACTCTCATGTCTTTGATTGCAACCATTCTTTACTACTTGGCTCATTCTAAGGCTGATACTGGCGGATGGGAGAAGCATCAGAACGATGGCATTAACATTGGTATGACCACTCCTTCAGATGCTCCAAAGCAACAAAACACCGAGTTTAACAAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

201

Amino Acids

21.41

Weight (kDa)

8.25

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 154 2.7e-25 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 205
AccI GTMKAC 1 cut(s) 155
AccII CGCG 1 cut(s) 356
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 4 cut(s) 5, 93, 203, 510
AclWI GGATC 2 cut(s) 117, 349
AcuI CTGAAG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 5 cut(s) 116, 140, 164, 259, 359
AfiI CCNNNNNNNGG 1 cut(s) 205
AflIII ACRYGT 1 cut(s) 354
AgsI TTSAA 1 cut(s) 18
AleI CACNNNNGTG 1 cut(s) 95
AluBI AGCT 4 cut(s) 309, 320, 397, 440
AluI AGCT 4 cut(s) 309, 320, 397, 440
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 2 cut(s) 117, 349
Aor13HI TCCGGA 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 431, 440
AsuHPI GGTGA 2 cut(s) 16, 35
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 3 cut(s) 52, 131, 277
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 418, 427
BccI CCATC 2 cut(s) 507, 527
BceAI ACGGC 2 cut(s) 69, 392
BcoDI GTCTC 1 cut(s) 233
BisI GCNGC 2 cut(s) 432, 441
BlsI GCNGC 2 cut(s) 433, 442
BmiI GGNNCC 2 cut(s) 73, 105
BmsI GCATC 2 cut(s) 532, 556
BpiI GAAGAC 3 cut(s) 52, 131, 277
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 152
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 259, 511
BseAI TCCGGA 1 cut(s) 106
BseGI GGATG 1 cut(s) 518
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 138, 312
BseNI ACTGG 2 cut(s) 259, 511
BseXI GCAGC 2 cut(s) 418, 427
Bsh1236I CGCG 1 cut(s) 356
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 101, 107
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 1 cut(s) 233
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 2 cut(s) 109, 341
BspACI CCGC 4 cut(s) 5, 93, 203, 510
BspCNI CTCAG 2 cut(s) 139, 313
BspEI TCCGGA 1 cut(s) 106
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 2 cut(s) 73, 105
BspPI GGATC 2 cut(s) 117, 349
BspQI GCTCTTC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 2 cut(s) 259, 511
BssAI RCCGGY 1 cut(s) 100
BssMI GATC 2 cut(s) 109, 341
BssNI GRCGYC 1 cut(s) 152
Bst4CI ACNGT 5 cut(s) 28, 160, 167, 215, 271
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 4 cut(s) 147, 222, 321, 495
BstF5I GGATG 1 cut(s) 518
BstFNI CGCG 1 cut(s) 356
BstKTI GATC 2 cut(s) 112, 344
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 2 cut(s) 109, 341
BstMWI GCNNNNNNNGC 4 cut(s) 71, 353, 437, 440
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 2 cut(s) 418, 427
BstV2I GAAGAC 3 cut(s) 52, 131, 277
BtsCI GGATG 1 cut(s) 518
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 3 cut(s) 33, 165, 267
Cac8I GCNNGC 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 100
CseI GACGC 2 cut(s) 68, 141
Csp6I GTAC 5 cut(s) 115, 139, 163, 258, 358
CviAII CATG 1 cut(s) 454
CviJI RGCY 8 cut(s) 198, 278, 309, 320, 397, 440, 488, 500
CviKI_1 RGCY 8 cut(s) 198, 278, 309, 320, 397, 440, 488, 500
CviQI GTAC 5 cut(s) 115, 139, 163, 258, 358
DdeI CTNAG 4 cut(s) 147, 222, 321, 495
DpnI GATC 2 cut(s) 111, 343
DpnII GATC 2 cut(s) 109, 341
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 525
Eco57I CTGAAG 1 cut(s) 546
FaeI CATG 1 cut(s) 457
FaiI YATR 3 cut(s) 170, 455, 551
FatI CATG 1 cut(s) 453
FblI GTMKAC 1 cut(s) 155
Fnu4HI GCNGC 2 cut(s) 432, 441
FokI GGATG 1 cut(s) 525
Fsp4HI GCNGC 2 cut(s) 432, 441
GluI GCNGC 2 cut(s) 432, 441
GsuI CTGGAG 1 cut(s) 456
HapII CCGG 2 cut(s) 101, 107
HgaI GACGC 2 cut(s) 68, 141
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 1 cut(s) 457
HincII GTYRAC 2 cut(s) 156, 211
HindII GTYRAC 2 cut(s) 156, 211
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 1 cut(s) 423
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 2 cut(s) 101, 107
HphI GGTGA 2 cut(s) 16, 35
Hpy166II GTNNAC 3 cut(s) 156, 163, 211
Hpy188I TCNGA 2 cut(s) 528, 565
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 3 cut(s) 156, 163, 211
Hpy99I CGWCG 3 cut(s) 32, 84, 157
HpyAV CCTTC 3 cut(s) 76, 368, 570
HpyCH4III ACNGT 5 cut(s) 28, 160, 167, 215, 271
HpyCH4V TGCA 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 353, 437, 440
HpyF3I CTNAG 4 cut(s) 147, 222, 321, 495
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 1 cut(s) 457
Kpn2I TCCGGA 1 cut(s) 106
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 109, 341
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 3 cut(s) 317, 437, 574
LpnPI CCDG 6 cut(s) 114, 120, 240, 259, 420, 492
Lsp1109I GCAGC 2 cut(s) 418, 427
LweI GCATC 2 cut(s) 532, 556
MaeIII GTNAC 3 cut(s) 31, 217, 250
MalI GATC 2 cut(s) 111, 343
MboI GATC 2 cut(s) 109, 341
MboII GAAGA 5 cut(s) 32, 52, 136, 277, 280
MluI ACGCGT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 432
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 5 cut(s) 32, 56, 142, 316, 342
MroI TCCGGA 1 cut(s) 106
MseI TTAA 3 cut(s) 210, 540, 594
MslI CAYNNNNRTG 3 cut(s) 95, 452, 548
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 2 cut(s) 101, 107
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 71, 353, 437, 440
NdeII GATC 2 cut(s) 109, 341
NlaIII CATG 1 cut(s) 457
NlaIV GGNNCC 2 cut(s) 73, 105
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 45
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 2 cut(s) 433, 442
PleI GAGTC 1 cut(s) 431
PpsI GAGTC 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 73, 105
RsaI GTAC 5 cut(s) 116, 140, 164, 259, 359
RsaNI GTAC 5 cut(s) 115, 139, 163, 258, 358
RseI CAYNNNNRTG 3 cut(s) 95, 452, 548
SalI GTCGAC 1 cut(s) 154
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 3 cut(s) 210, 540, 594
SatI GCNGC 2 cut(s) 432, 441
Sau3AI GATC 2 cut(s) 109, 341
SchI GAGTC 1 cut(s) 432
SetI ASST 6 cut(s) 48, 311, 322, 399, 442, 603
SfaNI GCATC 2 cut(s) 532, 556
SmiMI CAYNNNNRTG 3 cut(s) 95, 452, 548
SsiI CCGC 4 cut(s) 5, 93, 203, 510
TaaI ACNGT 5 cut(s) 28, 160, 167, 215, 271
TaqI TCGA 1 cut(s) 155
Tru1I TTAA 3 cut(s) 210, 540, 594
Tru9I TTAA 3 cut(s) 210, 540, 594
TscAI CASTG 3 cut(s) 40, 165, 274
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 431, 440
Tsp45I GTSAC 1 cut(s) 31
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 3 cut(s) 40, 165, 274
Van91I CCANNNNNTGG 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.