MD13G1035500.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007471
Physical Location & Seq
Reverse (-)
2490785 .. 2492934
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1035500.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCAGTCTCGATACCCATAGTCGCCATCGTTACCTCCCTCCATCTCATCGCCTTCATCTTCGCCGTCGGCGCCGAACGACGCCGTAGCACGGCGAAGATAGTGCCCGATGAGTACGACGAGCGTACCTACTGCGTGTACGGCACGGATGCCTCCACGGTGTACGGACTAGCGGCGTTTGGGCTGCTTGCCGTCAGCCAGACGGTGCTTAACGTCGTCACCAGGTGTCTCTGCTGCGGCAAAGGTCTAGTCACTGGCTCCTCCACCACTTGGACCGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCGGAGGCGTGCTTGTTGGCTGGGTCGGCAAAGAATGCATACCACACCAAGTACCGGGGAATCTTCAAAGTAGATGACTTGTCCTGTGCTACTCTGCGCAAGGGCGTGTTTGCCGCCGGCGCTGCTCTTACGCTGTTGTCACTGGCAGGGTCAAGTCTTTACTACTGGGCGCATTCCAAAGCTGATACTGGGGGATGGGAGAAGCACCGAAACGAAGGCCTTGACATGAGCAGTTCTAACTACGGGCAGCACGAGCAGCATGAACAACAAACCAGTGGATTTCAGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

203

Amino Acids

21.89

Weight (kDa)

8.2

Isoelectric Point (pI)

21.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 153 3.1e-27 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 317
AciI CCGC 4 cut(s) 173, 237, 317, 429
AcyI GRCGYC 2 cut(s) 72, 82
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 5 cut(s) 116, 127, 140, 164, 368
AfiI CCNNNNNNNGG 3 cut(s) 91, 270, 369
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 221, 296
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw26I GTCTC 2 cut(s) 13, 233
AoxI GGCC 1 cut(s) 532
ApeKI GCWGC 5 cut(s) 184, 234, 437, 562, 571
ArsI GACNNNNNNTTYG 4 cut(s) 234, 266, 292, 324
AspLEI GCGC 4 cut(s) 74, 414, 437, 487
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 108
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BanI GGYRCC 1 cut(s) 71
BauI CACGAG 1 cut(s) 566
BbsI GAAGAC 2 cut(s) 271, 283
BbvI GCAGC 5 cut(s) 171, 221, 424, 574, 583
BccI CCATC 3 cut(s) 35, 51, 504
BceAI ACGGC 5 cut(s) 50, 69, 108, 157, 176
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 2 cut(s) 223, 298
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 13, 233
BfaI CTAG 2 cut(s) 170, 248
BfoI RGCGCY 2 cut(s) 75, 438
BisI GCNGC 8 cut(s) 174, 185, 235, 238, 429, 438, 563, 572
BlsI GCNGC 8 cut(s) 175, 186, 236, 239, 430, 439, 564, 573
Bme1390I CCNGG 3 cut(s) 223, 298, 371
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 73, 259
BmrFI CCNGG 3 cut(s) 223, 298, 371
BmrI ACTGGG 2 cut(s) 490, 513
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 2 cut(s) 490, 513
BpiI GAAGAC 2 cut(s) 271, 283
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 2 cut(s) 72, 82
BsaJI CCNNGG 2 cut(s) 156, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 270, 369
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 5 cut(s) 259, 462, 485, 508, 588
BseBI CCWGG 2 cut(s) 223, 298
BseDI CCNNGG 2 cut(s) 156, 370
BseGI GGATG 2 cut(s) 154, 515
BseLI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BseNI ACTGG 5 cut(s) 259, 462, 485, 508, 588
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 5 cut(s) 171, 221, 424, 574, 583
BseYI CCCAGC 1 cut(s) 335
BshFI GGCC 1 cut(s) 534
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 370, 432
BslI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BsmAI GTCTC 2 cut(s) 13, 233
BsmI GAATGC 2 cut(s) 355, 487
BsnI GGCC 1 cut(s) 534
Bsp1286I GDGCHC 1 cut(s) 108
BspACI CCGC 4 cut(s) 173, 237, 317, 429
BspANI GGCC 1 cut(s) 534
BspLI GGNNCC 2 cut(s) 73, 259
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 5 cut(s) 259, 462, 485, 508, 588
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 156, 370
BssNI GRCGYC 2 cut(s) 72, 82
BssSI CACGAG 1 cut(s) 566
Bst2BI CACGAG 1 cut(s) 566
Bst2UI CCWGG 2 cut(s) 223, 298
Bst4CI ACNGT 3 cut(s) 160, 205, 277
BstACI GRCGYC 2 cut(s) 72, 82
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 3 cut(s) 189, 325, 433
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 154, 515
BstH2I RGCGCY 2 cut(s) 75, 438
BstHHI GCGC 4 cut(s) 74, 414, 437, 487
BstMAI GTCTC 2 cut(s) 13, 233
BstMWI GCNNNNNNNGC 8 cut(s) 71, 141, 341, 350, 434, 437, 568, 571
BstNI CCWGG 2 cut(s) 223, 298
BstSCI CCNGG 3 cut(s) 221, 296, 369
BstSLI GKGCMC 1 cut(s) 108
BstV1I GCAGC 5 cut(s) 171, 221, 424, 574, 583
BstV2I GAAGAC 2 cut(s) 271, 283
BsuRI GGCC 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 154, 515
BtsIMutI CAGTG 3 cut(s) 252, 455, 595
Cac8I GCNNGC 3 cut(s) 189, 325, 433
CfoI GCGC 4 cut(s) 74, 414, 437, 487
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 1 cut(s) 90
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 5 cut(s) 115, 126, 139, 163, 367
CviAII CATG 2 cut(s) 541, 575
CviJI RGCY 6 cut(s) 184, 198, 258, 335, 497, 534
CviKI_1 RGCY 6 cut(s) 184, 198, 258, 335, 497, 534
CviQI GTAC 5 cut(s) 115, 126, 139, 163, 367
DinI GGCGCC 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 221, 296
EcoT22I ATGCAT 1 cut(s) 355
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
FaeI CATG 2 cut(s) 544, 578
FaiI YATR 5 cut(s) 20, 355, 542, 576, 607
FatI CATG 2 cut(s) 540, 574
Fnu4HI GCNGC 8 cut(s) 174, 185, 235, 238, 429, 438, 563, 572
FokI GGATG 2 cut(s) 161, 522
Fsp4HI GCNGC 8 cut(s) 174, 185, 235, 238, 429, 438, 563, 572
FspBI CTAG 2 cut(s) 170, 248
FspI TGCGCA 1 cut(s) 413
GlaI GCGC 4 cut(s) 73, 413, 436, 486
GluI GCNGC 8 cut(s) 174, 185, 235, 238, 429, 438, 563, 572
GsaI CCCAGC 1 cut(s) 339
HaeII RGCGCY 2 cut(s) 75, 438
HaeIII GGCC 1 cut(s) 534
HapII CCGG 2 cut(s) 370, 432
HgaI GACGC 1 cut(s) 90
HhaI GCGC 4 cut(s) 74, 414, 437, 487
Hin1I GRCGYC 2 cut(s) 72, 82
Hin1II CATG 2 cut(s) 544, 578
Hin6I GCGC 4 cut(s) 72, 412, 435, 485
HinP1I GCGC 4 cut(s) 72, 412, 435, 485
HinfI GANTC 1 cut(s) 375
HpaII CCGG 2 cut(s) 370, 432
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 139, 163
Hpy188I TCNGA 1 cut(s) 600
Hpy188III TCNNGA 1 cut(s) 10
Hpy8I GTNNAC 2 cut(s) 139, 163
Hpy99I CGWCG 4 cut(s) 71, 84, 122, 218
HpyAV CCTTC 3 cut(s) 64, 524, 595
HpyCH4III ACNGT 3 cut(s) 160, 205, 277
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 141, 341, 350, 434, 437, 568, 571
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 72, 82
Hsp92II CATG 2 cut(s) 544, 578
HspAI GCGC 4 cut(s) 72, 412, 435, 485
KasI GGCGCC 1 cut(s) 71
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
LmnI GCTCC 2 cut(s) 263, 314
Lsp1109I GCAGC 5 cut(s) 171, 221, 424, 574, 583
LweI GCATC 1 cut(s) 139
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 2 cut(s) 170, 248
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 31, 217, 250, 453
MbiI CCGCTC 1 cut(s) 317
MboII GAAGA 7 cut(s) 52, 109, 271, 274, 277, 283, 370
MhlI GDGCHC 1 cut(s) 108
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 5 cut(s) 46, 50, 163, 271, 313
Mph1103I ATGCAT 1 cut(s) 355
MreI CGCCGGCG 1 cut(s) 431
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 1 cut(s) 210
MspI CCGG 2 cut(s) 370, 432
MspR9I CCNGG 3 cut(s) 223, 298, 371
Mva1269I GAATGC 2 cut(s) 355, 487
MvaI CCWGG 2 cut(s) 223, 298
MwoI GCNNNNNNNGC 8 cut(s) 71, 141, 341, 350, 434, 437, 568, 571
NaeI GCCGGC 1 cut(s) 433
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 371
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 2 cut(s) 544, 578
NlaIV GGNNCC 2 cut(s) 73, 259
NmuCI GTSAC 3 cut(s) 217, 250, 453
NsbI TGCGCA 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 355
PceI AGGCCT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 564
PctI GAATGC 2 cut(s) 355, 487
PdiI GCCGGC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 8 cut(s) 175, 186, 236, 239, 430, 439, 564, 573
PluTI GGCGCC 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 221, 296
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 221, 296
PspN4I GGNNCC 2 cut(s) 73, 259
PspPI GGNCC 1 cut(s) 273
RsaI GTAC 5 cut(s) 116, 127, 140, 164, 368
RsaNI GTAC 5 cut(s) 115, 126, 139, 163, 367
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 8 cut(s) 174, 185, 235, 238, 429, 438, 563, 572
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 3 cut(s) 223, 298, 371
SduI GDGCHC 1 cut(s) 108
SetI ASST 8 cut(s) 38, 131, 216, 227, 247, 308, 499, 606
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 1 cut(s) 139
SfoI GGCGCC 1 cut(s) 73
SgrAI CRCCGGYG 1 cut(s) 431
SinI GGWCC 1 cut(s) 273
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 4 cut(s) 173, 237, 317, 429
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 2 cut(s) 170, 248
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 3 cut(s) 221, 296, 369
TaaI ACNGT 3 cut(s) 160, 205, 277
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 11
TauI GCSGC 3 cut(s) 176, 240, 431
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 3 cut(s) 259, 462, 595
TseFI GTSAC 3 cut(s) 217, 250, 453
TseI GCWGC 5 cut(s) 184, 234, 437, 562, 571
Tsp45I GTSAC 3 cut(s) 217, 250, 453
TspDTI ATGAA 2 cut(s) 46, 591
TspGWI ACGGA 2 cut(s) 161, 180
TspRI CASTG 3 cut(s) 259, 462, 595
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 273
XspI CTAG 2 cut(s) 170, 248
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.