Rh4BG340100

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007471
Physical Location & Seq
Reverse (-)
50208883 .. 50211228
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG340100.1

Sequence Viewer

Length: 612 bp
ATGGCAGTCTCCGTTCCCATAGTGGCTATCATCACCTCTCTCCACCTCATCGCCTTCATTTTCGCCGTCGGCGCCGAACGACGCCGTAGCACCGCGAAGATAGTGCCCGACGAGTACGACGAGCAAACCTACTGCGTTTACGGCACCGACGCTTCCACCGTGTACGGTTTGGCCGCGTTCGGGCTTCTTCTGCTCAGCCACACGGTGCTTAACGGCGTCACCAGGTGTCTCTGCTTTGGCAAAGGCTTAATCACCGGCTCCTCTACTACTTGGACTGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCCGAGGCATGCTTATTGGCAGGGTCAGCAAGGAATGCCTATCACACAAAGTACAGGGGGAAATTTAACCTAGACGACTTGTCCTGTGCCACTCTCCGTAAGGGTGTGTTCGCTGCAGCAGCTGCTCTTACACTGTTGTCGCTGATAGGGTCAAACCTTTACTACTGGACACATTCCAAAGCTGATACTGGAGGGTGGGAGAAGCACCGCAATGAAGGCCTTGGCATGGGCAATTCCAATTATGGGCAGCATGAGCAGCAGCAGGAGCAGCAAACCACTGGATTCGAGAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

203

Amino Acids

22.17

Weight (kDa)

7.67

Isoelectric Point (pI)

22.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 153 2.1e-26 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 71, 143
AccII CGCG 2 cut(s) 95, 176
AciI CCGC 3 cut(s) 93, 174, 523
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 377
AcyI GRCGYC 3 cut(s) 72, 82, 216
AdeI CACNNNGTG 2 cut(s) 205, 225
AfaI GTAC 3 cut(s) 116, 164, 368
AfiI CCNNNNNNNGG 3 cut(s) 180, 541, 558
AhdI GACNNNNNGTC 1 cut(s) 394
AjnI CCWGG 2 cut(s) 221, 296
AluBI AGCT 2 cut(s) 437, 497
AluI AGCT 2 cut(s) 437, 497
Alw26I GTCTC 2 cut(s) 13, 233
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 2 cut(s) 171, 532
ApeKI GCWGC 8 cut(s) 428, 431, 434, 437, 562, 571, 574, 583
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
AspLEI GCGC 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 25, 211, 244
BaeGI GKGCMC 1 cut(s) 108
BanI GGYRCC 2 cut(s) 71, 143
BbsI GAAGAC 2 cut(s) 271, 283
BbvI GCAGC 8 cut(s) 415, 424, 443, 446, 574, 583, 586, 595
BceAI ACGGC 4 cut(s) 50, 69, 157, 229
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 2 cut(s) 223, 298
BcoDI GTCTC 2 cut(s) 13, 233
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 9 cut(s) 174, 429, 432, 435, 438, 563, 572, 575, 584
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 9 cut(s) 175, 430, 433, 436, 439, 564, 573, 576, 585
Bme1390I CCNGG 2 cut(s) 223, 298
BmeRI GACNNNNNGTC 1 cut(s) 394
BmiI GGNNCC 4 cut(s) 73, 145, 259, 316
BmrFI CCNGG 2 cut(s) 223, 298
BpiI GAAGAC 2 cut(s) 271, 283
BpmI CTGGAG 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 194
BsaHI GRCGYC 3 cut(s) 72, 82, 216
BsaJI CCNNGG 2 cut(s) 318, 535
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 541, 558
Bse118I RCCGGY 1 cut(s) 254
Bse1I ACTGG 3 cut(s) 485, 508, 598
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 2 cut(s) 223, 298
BseDI CCNNGG 2 cut(s) 318, 535
BseLI CCNNNNNNNGG 3 cut(s) 180, 541, 558
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 1 cut(s) 208
BseNI ACTGG 3 cut(s) 485, 508, 598
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 8 cut(s) 415, 424, 443, 446, 574, 583, 586, 595
Bsh1236I CGCG 2 cut(s) 95, 176
BshFI GGCC 2 cut(s) 173, 534
BshNI GGYRCC 2 cut(s) 71, 143
BsiSI CCGG 1 cut(s) 255
BslI CCNNNNNNNGG 3 cut(s) 180, 541, 558
BsmAI GTCTC 2 cut(s) 13, 233
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 2 cut(s) 173, 534
Bsp1286I GDGCHC 1 cut(s) 108
Bsp1720I GCTNAGC 1 cut(s) 194
BspACI CCGC 3 cut(s) 93, 174, 523
BspANI GGCC 2 cut(s) 173, 534
BspCNI CTCAG 1 cut(s) 207
BspFNI CGCG 2 cut(s) 95, 176
BspLI GGNNCC 4 cut(s) 73, 145, 259, 316
BspMAI CTGCAG 1 cut(s) 433
BspT107I GGYRCC 2 cut(s) 71, 143
BsrDI GCAATG 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 254
BsrI ACTGG 3 cut(s) 485, 508, 598
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 318, 535
BssNI GRCGYC 3 cut(s) 72, 82, 216
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 2 cut(s) 223, 298
Bst4CI ACNGT 5 cut(s) 160, 167, 205, 277, 450
BstACI GRCGYC 3 cut(s) 72, 82, 216
BstAPI GCANNNNNTGC 2 cut(s) 350, 437
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 1 cut(s) 194
BstFNI CGCG 2 cut(s) 95, 176
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstMAI GTCTC 2 cut(s) 13, 233
BstNI CCWGG 2 cut(s) 223, 298
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 2 cut(s) 221, 296
BstSFI CTRYAG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 2 cut(s) 95, 176
BstV1I GCAGC 8 cut(s) 415, 424, 443, 446, 574, 583, 586, 595
BstV2I GAAGAC 2 cut(s) 271, 283
BsuRI GGCC 2 cut(s) 173, 534
BtgZI GCGATG 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 446, 591
Cac8I GCNNGC 1 cut(s) 325
CaiI CAGNNNCTG 1 cut(s) 437
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 254
CseI GACGC 3 cut(s) 90, 158, 205
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 3 cut(s) 115, 163, 367
CviAII CATG 3 cut(s) 324, 541, 566
CviQI GTAC 3 cut(s) 115, 163, 367
DdeI CTNAG 1 cut(s) 194
DinI GGCGCC 1 cut(s) 73
DraIII CACNNNGTG 2 cut(s) 205, 225
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 535
Eco147I AGGCCT 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 221, 296
EcoT14I CCWWGG 1 cut(s) 535
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 3 cut(s) 327, 544, 569
FaiI YATR 5 cut(s) 20, 325, 542, 558, 567
FatI CATG 3 cut(s) 323, 540, 565
Fnu4HI GCNGC 9 cut(s) 174, 429, 432, 435, 438, 563, 572, 575, 584
Fsp4HI GCNGC 9 cut(s) 174, 429, 432, 435, 438, 563, 572, 575, 584
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 73
GluI GCNGC 9 cut(s) 174, 429, 432, 435, 438, 563, 572, 575, 584
GsuI CTGGAG 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 173, 534
HapII CCGG 1 cut(s) 255
HgaI GACGC 3 cut(s) 90, 158, 205
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 3 cut(s) 72, 82, 216
Hin1II CATG 3 cut(s) 327, 544, 569
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 1 cut(s) 597
HpaII CCGG 1 cut(s) 255
HphI GGTGA 3 cut(s) 25, 211, 244
Hpy166II GTNNAC 2 cut(s) 139, 163
Hpy188III TCNNGA 1 cut(s) 601
Hpy8I GTNNAC 2 cut(s) 139, 163
Hpy99I CGWCG 5 cut(s) 71, 84, 113, 122, 152
HpyAV CCTTC 3 cut(s) 64, 524, 598
HpyCH4III ACNGT 5 cut(s) 160, 167, 205, 277, 450
HpyCH4V TGCA 1 cut(s) 431
HpyF3I CTNAG 1 cut(s) 194
Hsp92I GRCGYC 3 cut(s) 72, 82, 216
Hsp92II CATG 3 cut(s) 327, 544, 569
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
LmnI GCTCC 3 cut(s) 263, 314, 580
Lsp1109I GCAGC 8 cut(s) 415, 424, 443, 446, 574, 583, 586, 595
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 217
MboII GAAGA 6 cut(s) 109, 179, 271, 274, 277, 283
MhlI GDGCHC 1 cut(s) 108
MluCI AATT 3 cut(s) 377, 547, 553
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 5 cut(s) 46, 56, 271, 313, 500
MseI TTAA 3 cut(s) 210, 248, 381
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 2 cut(s) 223, 298
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 223, 298
MvnI CGCG 2 cut(s) 95, 176
NarI GGCGCC 1 cut(s) 72
NlaIII CATG 3 cut(s) 327, 544, 569
NlaIV GGNNCC 4 cut(s) 73, 145, 259, 316
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 1 cut(s) 327
PaeI GCATGC 1 cut(s) 327
PceI AGGCCT 1 cut(s) 534
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 156
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 597
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 9 cut(s) 175, 430, 433, 436, 439, 564, 573, 576, 585
PluTI GGCGCC 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 221, 296
PspGI CCWGG 2 cut(s) 221, 296
PspN4I GGNNCC 4 cut(s) 73, 145, 259, 316
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 437
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 3 cut(s) 116, 164, 368
RsaNI GTAC 3 cut(s) 115, 163, 367
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 3 cut(s) 210, 248, 381
SatI GCNGC 9 cut(s) 174, 429, 432, 435, 438, 563, 572, 575, 584
ScrFI CCNGG 2 cut(s) 223, 298
SduI GDGCHC 1 cut(s) 108
SexAI ACCWGGT 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 429
SfoI GGCGCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 525
SphI GCATGC 1 cut(s) 327
Sse9I AATT 3 cut(s) 377, 547, 553
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 3 cut(s) 93, 174, 523
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 1 cut(s) 386
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 2 cut(s) 221, 296
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 5 cut(s) 160, 167, 205, 277, 450
TaqI TCGA 1 cut(s) 600
TasI AATT 3 cut(s) 377, 547, 553
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 597
Tru1I TTAA 3 cut(s) 210, 248, 381
Tru9I TTAA 3 cut(s) 210, 248, 381
TscAI CASTG 2 cut(s) 453, 598
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 8 cut(s) 428, 431, 434, 437, 562, 571, 574, 583
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 46, 543
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 453, 598
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 327
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.