MD16G1037500.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007471
Physical Location & Seq
Reverse (-)
2698335 .. 2699951
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1037500.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGCAGTCTCAATACCCATAGTCGCCATCGTCACCTCCCTCCACCTCATCGCCTTCATCTTTGCCGTCGGCGCTGAACGACGCCGTAGCACGGCGAAGATAGTGCCCGATGAGTACGACGAGCGTACCTACTGCGTGTACGGCACGGACGCCTCCACGGTGTACGGATTAGCGGCGTGTGGGCTGCTTCTGGTCAGCCAGGCGGTGATTAACGGCGTCACCAGGTGTCTCTGCTGCGGCAAAGGTCTTGTCACTGGCTCCTCCACCACTTGGACCGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCGGAGGCGTGCTTGTTGGCTGGGTCGGCAAAGAATGCATACCACACCAAGTACCGGGGAATCTTCAATGCAGATGACTTGTCTTGTTCTACTCTGCGCAAAGGGGTGTTTGCCGCCGGTGCTGCTCTTACACTGTTGTCACTGGCAGGGTCAAGTCTTTACTACTGGGCACATTCCAAAGCTGATACTGGGGGATGGGAGAAGCACCGAAATGAAGGCCTTGACATGAGCAGTTCTAACTACGGGCAGCATGAGCAGCAGCAACAACAAGCCAGTGGATTTCAGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

203

Amino Acids

21.75

Weight (kDa)

8.15

Isoelectric Point (pI)

25.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 153 1.5e-26 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 317
AciI CCGC 5 cut(s) 173, 203, 237, 317, 429
AcyI GRCGYC 3 cut(s) 82, 150, 216
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 5 cut(s) 116, 127, 140, 164, 368
AfiI CCNNNNNNNGG 3 cut(s) 91, 270, 369
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 3 cut(s) 198, 221, 296
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw26I GTCTC 2 cut(s) 13, 233
AoxI GGCC 1 cut(s) 532
ApeKI GCWGC 6 cut(s) 184, 234, 437, 562, 571, 574
ArsI GACNNNNNNTTYG 4 cut(s) 234, 266, 292, 324
AspLEI GCGC 2 cut(s) 74, 414
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 25, 211, 217
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 2 cut(s) 108, 487
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BbsI GAAGAC 2 cut(s) 271, 283
BbvI GCAGC 6 cut(s) 171, 221, 424, 574, 583, 586
BccI CCATC 2 cut(s) 35, 504
BceAI ACGGC 5 cut(s) 50, 69, 108, 157, 229
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 3 cut(s) 200, 223, 298
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 13, 233
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 9 cut(s) 174, 185, 235, 238, 429, 438, 563, 572, 575
BlsI GCNGC 9 cut(s) 175, 186, 236, 239, 430, 439, 564, 573, 576
Bme1390I CCNGG 4 cut(s) 200, 223, 298, 371
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 4 cut(s) 200, 223, 298, 371
BmrI ACTGGG 2 cut(s) 490, 513
BmuI ACTGGG 2 cut(s) 490, 513
BpiI GAAGAC 2 cut(s) 271, 283
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 3 cut(s) 82, 150, 216
BsaJI CCNNGG 2 cut(s) 156, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 270, 369
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 5 cut(s) 259, 462, 485, 508, 588
BseBI CCWGG 3 cut(s) 200, 223, 298
BseDI CCNNGG 2 cut(s) 156, 370
BseGI GGATG 1 cut(s) 515
BseLI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BseNI ACTGG 5 cut(s) 259, 462, 485, 508, 588
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 2 cut(s) 108, 487
BseXI GCAGC 6 cut(s) 171, 221, 424, 574, 583, 586
BseYI CCCAGC 1 cut(s) 335
BshFI GGCC 1 cut(s) 534
BsiSI CCGG 2 cut(s) 370, 432
BslI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BsmAI GTCTC 2 cut(s) 13, 233
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 108, 487
BspACI CCGC 5 cut(s) 173, 203, 237, 317, 429
BspANI GGCC 1 cut(s) 534
BspLI GGNNCC 1 cut(s) 259
BsrBI CCGCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 5 cut(s) 259, 462, 485, 508, 588
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 156, 370
BssNI GRCGYC 3 cut(s) 82, 150, 216
Bst2UI CCWGG 3 cut(s) 200, 223, 298
Bst4CI ACNGT 3 cut(s) 160, 277, 450
BstACI GRCGYC 3 cut(s) 82, 150, 216
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 1 cut(s) 325
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 515
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 414
BstMAI GTCTC 2 cut(s) 13, 233
BstMWI GCNNNNNNNGC 8 cut(s) 71, 141, 341, 350, 434, 437, 568, 571
BstNI CCWGG 3 cut(s) 200, 223, 298
BstSCI CCNGG 4 cut(s) 198, 221, 296, 369
BstSLI GKGCMC 2 cut(s) 108, 487
BstV1I GCAGC 6 cut(s) 171, 221, 424, 574, 583, 586
BstV2I GAAGAC 2 cut(s) 271, 283
BsuRI GGCC 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 515
BtsIMutI CAGTG 4 cut(s) 252, 446, 455, 595
Cac8I GCNNGC 1 cut(s) 325
CfoI GCGC 2 cut(s) 74, 414
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 3 cut(s) 90, 158, 205
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 5 cut(s) 115, 126, 139, 163, 367
CviAII CATG 2 cut(s) 541, 566
CviJI RGCY 7 cut(s) 184, 198, 258, 335, 497, 534, 587
CviKI_1 RGCY 7 cut(s) 184, 198, 258, 335, 497, 534, 587
CviQI GTAC 5 cut(s) 115, 126, 139, 163, 367
DraIII CACNNNGTG 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 273
EcoRII CCWGG 3 cut(s) 198, 221, 296
EcoT22I ATGCAT 1 cut(s) 355
FaeI CATG 2 cut(s) 544, 569
FaiI YATR 5 cut(s) 20, 355, 542, 567, 607
FatI CATG 2 cut(s) 540, 565
Fnu4HI GCNGC 9 cut(s) 174, 185, 235, 238, 429, 438, 563, 572, 575
FokI GGATG 1 cut(s) 522
Fsp4HI GCNGC 9 cut(s) 174, 185, 235, 238, 429, 438, 563, 572, 575
FspI TGCGCA 1 cut(s) 413
GlaI GCGC 2 cut(s) 73, 413
GluI GCNGC 9 cut(s) 174, 185, 235, 238, 429, 438, 563, 572, 575
GsaI CCCAGC 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 534
HapII CCGG 2 cut(s) 370, 432
HgaI GACGC 3 cut(s) 90, 158, 205
HhaI GCGC 2 cut(s) 74, 414
Hin1I GRCGYC 3 cut(s) 82, 150, 216
Hin1II CATG 2 cut(s) 544, 569
Hin6I GCGC 2 cut(s) 72, 412
HinP1I GCGC 2 cut(s) 72, 412
HinfI GANTC 1 cut(s) 375
HpaII CCGG 2 cut(s) 370, 432
HphI GGTGA 3 cut(s) 25, 211, 217
Hpy166II GTNNAC 2 cut(s) 139, 163
Hpy188I TCNGA 1 cut(s) 600
Hpy8I GTNNAC 2 cut(s) 139, 163
Hpy99I CGWCG 3 cut(s) 71, 84, 122
HpyAV CCTTC 3 cut(s) 64, 524, 595
HpyCH4III ACNGT 3 cut(s) 160, 277, 450
HpyCH4V TGCA 2 cut(s) 353, 386
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 141, 341, 350, 434, 437, 568, 571
Hsp92I GRCGYC 3 cut(s) 82, 150, 216
Hsp92II CATG 2 cut(s) 544, 569
HspAI GCGC 2 cut(s) 72, 412
LmnI GCTCC 2 cut(s) 263, 314
Lsp1109I GCAGC 6 cut(s) 171, 221, 424, 574, 583, 586
MabI ACCWGGT 1 cut(s) 221
MaeIII GTNAC 4 cut(s) 31, 217, 250, 453
MbiI CCGCTC 1 cut(s) 317
MboII GAAGA 6 cut(s) 109, 271, 274, 277, 283, 370
MhlI GDGCHC 2 cut(s) 108, 487
MnlI CCTC 6 cut(s) 46, 50, 56, 163, 271, 313
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 525
MspI CCGG 2 cut(s) 370, 432
MspR9I CCNGG 4 cut(s) 200, 223, 298, 371
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 3 cut(s) 200, 223, 298
MwoI GCNNNNNNNGC 8 cut(s) 71, 141, 341, 350, 434, 437, 568, 571
NciI CCSGG 1 cut(s) 371
NlaIII CATG 2 cut(s) 544, 569
NlaIV GGNNCC 1 cut(s) 259
NmuCI GTSAC 4 cut(s) 31, 217, 250, 453
NsbI TGCGCA 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 355
PceI AGGCCT 1 cut(s) 534
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 9 cut(s) 175, 186, 236, 239, 430, 439, 564, 573, 576
Psp6I CCWGG 3 cut(s) 198, 221, 296
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 3 cut(s) 198, 221, 296
PspN4I GGNNCC 1 cut(s) 259
PspPI GGNCC 1 cut(s) 273
RsaI GTAC 5 cut(s) 116, 127, 140, 164, 368
RsaNI GTAC 5 cut(s) 115, 126, 139, 163, 367
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 9 cut(s) 174, 185, 235, 238, 429, 438, 563, 572, 575
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 4 cut(s) 200, 223, 298, 371
SduI GDGCHC 2 cut(s) 108, 487
SetI ASST 8 cut(s) 38, 48, 131, 227, 247, 308, 499, 606
SexAI ACCWGGT 1 cut(s) 221
SgrAI CRCCGGYG 1 cut(s) 431
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 525
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 5 cut(s) 173, 203, 237, 317, 429
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 4 cut(s) 198, 221, 296, 369
TaaI ACNGT 3 cut(s) 160, 277, 450
TauI GCSGC 3 cut(s) 176, 240, 431
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 4 cut(s) 259, 453, 462, 595
TseFI GTSAC 4 cut(s) 31, 217, 250, 453
TseI GCWGC 6 cut(s) 184, 234, 437, 562, 571, 574
Tsp45I GTSAC 4 cut(s) 31, 217, 250, 453
TspDTI ATGAA 2 cut(s) 46, 543
TspGWI ACGGA 2 cut(s) 161, 180
TspRI CASTG 4 cut(s) 259, 453, 462, 595
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 273
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.