MD01G1086300.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007471
Physical Location & Seq
Reverse (-)
19476003 .. 19476395
393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1086300.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCAGTCTCGATACCCATAGTCGCCATCGTTACCTCCCTCCATCTCATCGCCTTCATCTTCGCCGTCGGCACCGAACGACGCCGTAGCACGGCAAAGATAGTGCCCGATGAGTACGACGAGCGTACCTACTGCGTGTACGGCACGGATGCCTCCACGGTGTACGGACTAGCGGCGTTTGGGCTGCTTGCCGTCAGCCAGACGGTGCTTAACGTCGTCACCAGGTGTCTCTGCTGCGGCAAAGGTCTAGTCACTGGCTCCTCCACCACTTGGACCGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCGGAGGCGTGCTTGTTGGCTGGGTCGGCAAAGAATGCATACCACACCAAGTACCGGGGAATCTTCAAAAGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

131

Amino Acids

14.14

Weight (kDa)

9.1

Isoelectric Point (pI)

19.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 124 1.1e-19 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 317
AciI CCGC 3 cut(s) 173, 237, 317
AcyI GRCGYC 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 5 cut(s) 116, 127, 140, 164, 368
AfiI CCNNNNNNNGG 3 cut(s) 91, 270, 369
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 221, 296
Alw26I GTCTC 2 cut(s) 13, 233
ApeKI GCWGC 2 cut(s) 184, 234
ArsI GACNNNNNNTTYG 4 cut(s) 234, 266, 292, 324
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 108
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 2 cut(s) 271, 283
BbvI GCAGC 2 cut(s) 171, 221
BccI CCATC 2 cut(s) 35, 51
BceAI ACGGC 5 cut(s) 50, 69, 108, 157, 176
BciT130I CCWGG 2 cut(s) 223, 298
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 13, 233
BfaI CTAG 2 cut(s) 170, 248
BisI GCNGC 4 cut(s) 174, 185, 235, 238
BlsI GCNGC 4 cut(s) 175, 186, 236, 239
Bme1390I CCNGG 3 cut(s) 223, 298, 371
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 73, 259
BmrFI CCNGG 3 cut(s) 223, 298, 371
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 271, 283
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 156, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 270, 369
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 2 cut(s) 223, 298
BseDI CCNNGG 2 cut(s) 156, 370
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 2 cut(s) 171, 221
BseYI CCCAGC 1 cut(s) 335
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BsmAI GTCTC 2 cut(s) 13, 233
BsmI GAATGC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 108
BspACI CCGC 3 cut(s) 173, 237, 317
BspLI GGNNCC 2 cut(s) 73, 259
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 317
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 2 cut(s) 156, 370
BssNI GRCGYC 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 223, 298
Bst4CI ACNGT 3 cut(s) 160, 205, 277
BstACI GRCGYC 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 189, 325
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 154
BstMAI GTCTC 2 cut(s) 13, 233
BstMWI GCNNNNNNNGC 3 cut(s) 141, 341, 350
BstNI CCWGG 2 cut(s) 223, 298
BstSCI CCNGG 3 cut(s) 221, 296, 369
BstSLI GKGCMC 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 171, 221
BstV2I GAAGAC 2 cut(s) 271, 283
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 189, 325
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 1 cut(s) 90
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 5 cut(s) 115, 126, 139, 163, 367
CviJI RGCY 4 cut(s) 184, 198, 258, 335
CviKI_1 RGCY 4 cut(s) 184, 198, 258, 335
CviQI GTAC 5 cut(s) 115, 126, 139, 163, 367
DraIII CACNNNGTG 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 221, 296
EcoT22I ATGCAT 1 cut(s) 355
FaiI YATR 2 cut(s) 20, 355
Fnu4HI GCNGC 4 cut(s) 174, 185, 235, 238
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 4 cut(s) 174, 185, 235, 238
FspBI CTAG 2 cut(s) 170, 248
GluI GCNGC 4 cut(s) 174, 185, 235, 238
GsaI CCCAGC 1 cut(s) 339
HapII CCGG 1 cut(s) 370
HgaI GACGC 1 cut(s) 90
Hin1I GRCGYC 1 cut(s) 82
HinfI GANTC 1 cut(s) 375
HpaII CCGG 1 cut(s) 370
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 139, 163
Hpy188III TCNNGA 1 cut(s) 10
Hpy8I GTNNAC 2 cut(s) 139, 163
Hpy99I CGWCG 4 cut(s) 71, 84, 122, 218
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 3 cut(s) 160, 205, 277
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 341, 350
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 82
LmnI GCTCC 2 cut(s) 263, 314
LpnPI CCDG 8 cut(s) 208, 212, 235, 240, 283, 310, 321, 383
Lsp1109I GCAGC 2 cut(s) 171, 221
LweI GCATC 1 cut(s) 139
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 2 cut(s) 170, 248
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 31, 217, 250
MbiI CCGCTC 1 cut(s) 317
MboII GAAGA 6 cut(s) 52, 271, 274, 277, 283, 370
MhlI GDGCHC 1 cut(s) 108
MnlI CCTC 5 cut(s) 46, 50, 163, 271, 313
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 1 cut(s) 210
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 3 cut(s) 223, 298, 371
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 223, 298
MwoI GCNNNNNNNGC 3 cut(s) 141, 341, 350
NciI CCSGG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 73, 259
NmuCI GTSAC 2 cut(s) 217, 250
NsiI ATGCAT 1 cut(s) 355
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 4 cut(s) 175, 186, 236, 239
Psp6I CCWGG 2 cut(s) 221, 296
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 221, 296
PspN4I GGNNCC 2 cut(s) 73, 259
PspPI GGNCC 1 cut(s) 273
RsaI GTAC 5 cut(s) 116, 127, 140, 164, 368
RsaNI GTAC 5 cut(s) 115, 126, 139, 163, 367
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 4 cut(s) 174, 185, 235, 238
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 3 cut(s) 223, 298, 371
SduI GDGCHC 1 cut(s) 108
SetI ASST 6 cut(s) 38, 131, 216, 227, 247, 308
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 1 cut(s) 139
SinI GGWCC 1 cut(s) 273
SsiI CCGC 3 cut(s) 173, 237, 317
SspMI CTAG 2 cut(s) 170, 248
StyD4I CCNGG 3 cut(s) 221, 296, 369
TaaI ACNGT 3 cut(s) 160, 205, 277
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 11
TauI GCSGC 2 cut(s) 176, 240
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 2 cut(s) 217, 250
TseI GCWGC 2 cut(s) 184, 234
Tsp45I GTSAC 2 cut(s) 217, 250
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 2 cut(s) 161, 180
TspRI CASTG 1 cut(s) 259
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 273
XspI CTAG 2 cut(s) 170, 248
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.