MD15G1416200.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007471
Physical Location & Seq
Forward (+)
51753536 .. 51754089
554 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1416200.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGCAGTCTCGATACCCATAGTCGCCATCGTTACCTCCCTCCATCTCATCGCCTTCATCTTCGCCGTCGGCACCGAACGACGCCGTAGCACGGCAAAGATAGTGCCCGATGAGTACGACGAGCGTACCTACTGCGTGTACGGCACGGATGCCTCCACGGTGTACGGACTAGCGGCGTTTGGGCTGCTTGCCGTCAGCCAGACGGTGCTTAACGTCGTCACCAGGTGTCTCTGCTGCGGCAAAGGTCTAGTCACTGGCTCCTCCACCACTTGGACCGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCGGAGGCGTGCTTGTTGGCTGGGTCGGCAAAGAATGCATACCACACCAAGTACCGGGGAATCTTCAAAGTAGTAGATGACTTGTCCTGTGCTACTCTGCGCAAGGGCATGTTTGCCGCCGGCACTGTTCTTACGCTGTTGTCACTGGCAGGATCAAGGGCGAGGTCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

163

Amino Acids

17.37

Weight (kDa)

8.98

Isoelectric Point (pI)

21.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 154 1.3e-26 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 317
AciI CCGC 4 cut(s) 173, 237, 317, 432
AclWI GGATC 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 5 cut(s) 116, 127, 140, 164, 368
AfiI CCNNNNNNNGG 3 cut(s) 91, 270, 369
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 221, 296
Alw26I GTCTC 2 cut(s) 13, 233
AlwI GGATC 1 cut(s) 475
ApeKI GCWGC 2 cut(s) 184, 234
ArsI GACNNNNNNTTYG 4 cut(s) 234, 266, 292, 324
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 108
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 2 cut(s) 271, 283
BbvI GCAGC 2 cut(s) 171, 221
BccI CCATC 2 cut(s) 35, 51
BceAI ACGGC 5 cut(s) 50, 69, 108, 157, 176
BciT130I CCWGG 2 cut(s) 223, 298
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 13, 233
BfaI CTAG 2 cut(s) 170, 248
BisI GCNGC 5 cut(s) 174, 185, 235, 238, 432
BlsI GCNGC 5 cut(s) 175, 186, 236, 239, 433
Bme1390I CCNGG 3 cut(s) 223, 298, 371
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 73, 259
BmrFI CCNGG 3 cut(s) 223, 298, 371
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 271, 283
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 156, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 270, 369
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 2 cut(s) 259, 465
BseBI CCWGG 2 cut(s) 223, 298
BseDI CCNNGG 2 cut(s) 156, 370
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BseNI ACTGG 2 cut(s) 259, 465
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 2 cut(s) 171, 221
BseYI CCCAGC 1 cut(s) 335
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 370, 435
BslI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BsmAI GTCTC 2 cut(s) 13, 233
BsmI GAATGC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 467
BspACI CCGC 4 cut(s) 173, 237, 317, 432
BspLI GGNNCC 2 cut(s) 73, 259
BspPI GGATC 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 2 cut(s) 259, 465
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 2 cut(s) 156, 370
BssMI GATC 1 cut(s) 467
BssNI GRCGYC 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 223, 298
Bst4CI ACNGT 4 cut(s) 160, 205, 277, 442
BstACI GRCGYC 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 3 cut(s) 189, 325, 436
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 1 cut(s) 470
BstMAI GTCTC 2 cut(s) 13, 233
BstMBI GATC 1 cut(s) 467
BstMWI GCNNNNNNNGC 3 cut(s) 141, 341, 350
BstNI CCWGG 2 cut(s) 223, 298
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 3 cut(s) 221, 296, 369
BstSLI GKGCMC 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 171, 221
BstV2I GAAGAC 2 cut(s) 271, 283
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 3 cut(s) 252, 438, 458
Cac8I GCNNGC 3 cut(s) 189, 325, 436
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 1 cut(s) 90
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 5 cut(s) 115, 126, 139, 163, 367
CviAII CATG 1 cut(s) 424
CviJI RGCY 4 cut(s) 184, 198, 258, 335
CviKI_1 RGCY 4 cut(s) 184, 198, 258, 335
CviQI GTAC 5 cut(s) 115, 126, 139, 163, 367
DpnI GATC 1 cut(s) 469
DpnII GATC 1 cut(s) 467
DraIII CACNNNGTG 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 221, 296
EcoT22I ATGCAT 1 cut(s) 355
FaeI CATG 1 cut(s) 427
FaiI YATR 3 cut(s) 20, 355, 425
FatI CATG 1 cut(s) 423
Fnu4HI GCNGC 5 cut(s) 174, 185, 235, 238, 432
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 174, 185, 235, 238, 432
FspBI CTAG 2 cut(s) 170, 248
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
GluI GCNGC 5 cut(s) 174, 185, 235, 238, 432
GsaI CCCAGC 1 cut(s) 339
HapII CCGG 2 cut(s) 370, 435
HgaI GACGC 1 cut(s) 90
HhaI GCGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 82
Hin1II CATG 1 cut(s) 427
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HinfI GANTC 1 cut(s) 375
HpaII CCGG 2 cut(s) 370, 435
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 139, 163
Hpy188I TCNGA 1 cut(s) 484
Hpy188III TCNNGA 1 cut(s) 10
Hpy8I GTNNAC 2 cut(s) 139, 163
Hpy99I CGWCG 4 cut(s) 71, 84, 122, 218
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 4 cut(s) 160, 205, 277, 442
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 341, 350
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 82
Hsp92II CATG 1 cut(s) 427
HspAI GCGC 1 cut(s) 415
KroI GCCGGC 1 cut(s) 434
KroNI GCCGGC 1 cut(s) 436
Kzo9I GATC 1 cut(s) 467
LmnI GCTCC 2 cut(s) 263, 314
Lsp1109I GCAGC 2 cut(s) 171, 221
LweI GCATC 1 cut(s) 139
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 2 cut(s) 170, 248
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 31, 217, 250, 456
MalI GATC 1 cut(s) 469
MbiI CCGCTC 1 cut(s) 317
MboI GATC 1 cut(s) 467
MboII GAAGA 6 cut(s) 52, 271, 274, 277, 283, 370
MhlI GDGCHC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 6 cut(s) 46, 50, 163, 271, 313, 471
Mph1103I ATGCAT 1 cut(s) 355
MroNI GCCGGC 1 cut(s) 434
MseI TTAA 1 cut(s) 210
MspI CCGG 2 cut(s) 370, 435
MspR9I CCNGG 3 cut(s) 223, 298, 371
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 223, 298
MwoI GCNNNNNNNGC 3 cut(s) 141, 341, 350
NaeI GCCGGC 1 cut(s) 436
NciI CCSGG 1 cut(s) 371
NdeII GATC 1 cut(s) 467
NgoMIV GCCGGC 1 cut(s) 434
NlaIII CATG 1 cut(s) 427
NlaIV GGNNCC 2 cut(s) 73, 259
NmuCI GTSAC 3 cut(s) 217, 250, 456
NsbI TGCGCA 1 cut(s) 416
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 1 cut(s) 427
PctI GAATGC 1 cut(s) 355
PdiI GCCGGC 1 cut(s) 436
PfeI GAWTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 5 cut(s) 175, 186, 236, 239, 433
Psp6I CCWGG 2 cut(s) 221, 296
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 221, 296
PspN4I GGNNCC 2 cut(s) 73, 259
PspPI GGNCC 1 cut(s) 273
RsaI GTAC 5 cut(s) 116, 127, 140, 164, 368
RsaNI GTAC 5 cut(s) 115, 126, 139, 163, 367
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 5 cut(s) 174, 185, 235, 238, 432
Sau3AI GATC 1 cut(s) 467
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 3 cut(s) 223, 298, 371
SduI GDGCHC 1 cut(s) 108
SetI ASST 7 cut(s) 38, 131, 216, 227, 247, 308, 482
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 1 cut(s) 139
SinI GGWCC 1 cut(s) 273
SsiI CCGC 4 cut(s) 173, 237, 317, 432
SspMI CTAG 2 cut(s) 170, 248
StyD4I CCNGG 3 cut(s) 221, 296, 369
TaaI ACNGT 4 cut(s) 160, 205, 277, 442
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 11
TauI GCSGC 3 cut(s) 176, 240, 434
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 3 cut(s) 259, 445, 465
TseFI GTSAC 3 cut(s) 217, 250, 456
TseI GCWGC 2 cut(s) 184, 234
Tsp45I GTSAC 3 cut(s) 217, 250, 456
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 2 cut(s) 161, 180
TspRI CASTG 3 cut(s) 259, 445, 465
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 273
XceI RCATGY 1 cut(s) 427
XspI CTAG 2 cut(s) 170, 248
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.