FvH4_4g26760

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007471
Physical Location & Seq
Reverse (-)
28029657 .. 28031806
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26760.t1

Sequence Viewer

Length: 603 bp
ATGGCGCTCTCCGTCCCCATAGTGGCCATCATCACCTCCCTCCACCTCATCGCCTTCATCTTCGCCGTCGGCGCCGAACGACGCCGTTCCACGGCTAAGATAGTCCCCGACGAGTACGATGAGCAAACCTACTGTGTTTACGGCACCGACGCTTCCACCGTCTATGGTTTGGCCGCGATTGGTCTTCTTCTTCTCAGCCACACCGTGCTTAACGCCGTCACCAGGTGTCTCTGCTGCGGCAAAGGCTTAGTCTCCGGCTCCTCCACTACTCGCACCGTCTTCTTCTTCGTCTTCTCCTGGACAACATTTTTGGGAGCTGAGGCATGCTTATTGGCCGGGTCAGCAAGGAATGCATACCACACAAAATACAGGGGGAAATTTAACCTAGATGACTTGTCCTGTGCCACTCTCCGTAAGGGCGTGTTTGCTGCAGCAGCTGCTCTTACACTGTTGTCGCTGATAGGGGCAAACCTGTACTATTGGTCACATTCCAAAGCTGATACCGGAGGGTGGGAGAAGCACCATAATGAGGGCCTTGGCATGTCTAACTATGGAGTGCATGAGCAGCAGCAGCAACAAACCACTGGATTCGAGAAGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

201

Amino Acids

21.69

Weight (kDa)

8.21

Isoelectric Point (pI)

28.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 153 2.2e-26 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 71, 143
AccII CGCG 1 cut(s) 176
AciI CCGC 2 cut(s) 174, 237
AcoI YGGCCR 3 cut(s) 24, 171, 333
AcsI RAATTY 1 cut(s) 377
AcyI GRCGYC 2 cut(s) 72, 82
AdeI CACNNNGTG 2 cut(s) 205, 225
AfaI GTAC 2 cut(s) 116, 476
AfiI CCNNNNNNNGG 5 cut(s) 22, 91, 222, 510, 529
AjnI CCWGG 2 cut(s) 221, 296
AluBI AGCT 3 cut(s) 317, 437, 497
AluI AGCT 3 cut(s) 317, 437, 497
Alw26I GTCTC 2 cut(s) 233, 256
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 4 cut(s) 24, 171, 333, 532
ApeKI GCWGC 8 cut(s) 234, 428, 431, 434, 437, 565, 568, 571
ApoI RAATTY 1 cut(s) 377
ArsI GACNNNNNNTTYG 4 cut(s) 234, 266, 292, 324
AspLEI GCGC 2 cut(s) 7, 74
AspS9I GGNCC 1 cut(s) 532
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 25, 211
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 2 cut(s) 71, 143
BbsI GAAGAC 3 cut(s) 176, 271, 283
BbvCI CCTCAGC 1 cut(s) 318
BbvI GCAGC 8 cut(s) 221, 415, 424, 443, 446, 577, 580, 583
BccI CCATC 1 cut(s) 35
BceAI ACGGC 5 cut(s) 50, 69, 108, 157, 200
BciT130I CCWGG 2 cut(s) 223, 298
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 2 cut(s) 233, 256
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 1 cut(s) 429
BfoI RGCGCY 2 cut(s) 8, 75
Bme1390I CCNGG 3 cut(s) 223, 298, 337
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 3 cut(s) 73, 145, 259
BmrFI CCNGG 3 cut(s) 223, 298, 337
BpiI GAAGAC 3 cut(s) 176, 271, 283
Bpu10I CCTNAGC 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 337
BsaHI GRCGYC 2 cut(s) 72, 82
BsaJI CCNNGG 2 cut(s) 90, 535
BsaWI WCCGGW 1 cut(s) 503
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 91, 222, 510, 529
Bse1I ACTGG 1 cut(s) 589
BseBI CCWGG 2 cut(s) 223, 298
BseDI CCNNGG 2 cut(s) 90, 535
BseLI CCNNNNNNNGG 5 cut(s) 22, 91, 222, 510, 529
BseMII CTCAG 2 cut(s) 208, 309
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 1 cut(s) 250
BseXI GCAGC 8 cut(s) 221, 415, 424, 443, 446, 577, 580, 583
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 4 cut(s) 26, 173, 335, 534
BshNI GGYRCC 2 cut(s) 71, 143
BsiSI CCGG 3 cut(s) 255, 336, 504
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 5 cut(s) 22, 91, 222, 510, 529
BsmAI GTCTC 2 cut(s) 233, 256
BsmFI GGGAC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 4 cut(s) 26, 173, 335, 534
BspACI CCGC 2 cut(s) 174, 237
BspANI GGCC 4 cut(s) 26, 173, 335, 534
BspCNI CTCAG 2 cut(s) 207, 310
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 73, 145, 259
BspMAI CTGCAG 1 cut(s) 433
BspT107I GGYRCC 2 cut(s) 71, 143
BsrI ACTGG 1 cut(s) 589
BssECI CCNNGG 2 cut(s) 90, 535
BssNI GRCGYC 2 cut(s) 72, 82
BssT1I CCWWGG 1 cut(s) 535
Bst2UI CCWGG 2 cut(s) 223, 298
Bst4CI ACNGT 5 cut(s) 134, 160, 205, 277, 450
BstACI GRCGYC 2 cut(s) 72, 82
BstAPI GCANNNNNTGC 2 cut(s) 350, 437
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 4 cut(s) 96, 194, 247, 318
BstDSI CCRYGG 1 cut(s) 90
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 2 cut(s) 8, 75
BstHHI GCGC 2 cut(s) 7, 74
BstMAI GTCTC 2 cut(s) 233, 256
BstMWI GCNNNNNNNGC 8 cut(s) 71, 243, 341, 350, 434, 437, 565, 571
BstNI CCWGG 2 cut(s) 223, 298
BstNSI RCATGY 2 cut(s) 327, 544
BstSCI CCNGG 3 cut(s) 221, 296, 335
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 8 cut(s) 221, 415, 424, 443, 446, 577, 580, 583
BstV2I GAAGAC 3 cut(s) 176, 271, 283
BsuRI GGCC 4 cut(s) 26, 173, 335, 534
BtgI CCRYGG 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 446, 582
Cac8I GCNNGC 1 cut(s) 325
CaiI CAGNNNCTG 1 cut(s) 437
CfoI GCGC 2 cut(s) 7, 74
Cfr13I GGNCC 1 cut(s) 532
CseI GACGC 2 cut(s) 90, 158
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 2 cut(s) 115, 475
CviAII CATG 3 cut(s) 324, 541, 560
CviQI GTAC 2 cut(s) 115, 475
DdeI CTNAG 4 cut(s) 96, 194, 247, 318
DinI GGCGCC 1 cut(s) 73
DraIII CACNNNGTG 2 cut(s) 205, 225
EaeI YGGCCR 3 cut(s) 24, 171, 333
Eco130I CCWWGG 1 cut(s) 535
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 2 cut(s) 221, 296
EcoT14I CCWWGG 1 cut(s) 535
EcoT22I ATGCAT 1 cut(s) 355
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 535
FaeI CATG 3 cut(s) 327, 544, 563
FaiI YATR 8 cut(s) 20, 165, 325, 355, 525, 542, 552, 561
FaqI GGGAC 1 cut(s) 89
FatI CATG 3 cut(s) 323, 540, 559
FspBI CTAG 1 cut(s) 386
GlaI GCGC 2 cut(s) 6, 73
HaeII RGCGCY 2 cut(s) 8, 75
HaeIII GGCC 4 cut(s) 26, 173, 335, 534
HapII CCGG 3 cut(s) 255, 336, 504
HgaI GACGC 2 cut(s) 90, 158
HhaI GCGC 2 cut(s) 7, 74
Hin1I GRCGYC 2 cut(s) 72, 82
Hin1II CATG 3 cut(s) 327, 544, 563
Hin6I GCGC 2 cut(s) 5, 72
HinP1I GCGC 2 cut(s) 5, 72
HinfI GANTC 1 cut(s) 588
HpaII CCGG 3 cut(s) 255, 336, 504
HphI GGTGA 2 cut(s) 25, 211
Hpy166II GTNNAC 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 592
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 4 cut(s) 71, 84, 113, 152
HpyAV CCTTC 2 cut(s) 64, 589
HpyCH4III ACNGT 5 cut(s) 134, 160, 205, 277, 450
HpyCH4V TGCA 3 cut(s) 353, 431, 559
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 243, 341, 350, 434, 437, 565, 571
HpyF3I CTNAG 4 cut(s) 96, 194, 247, 318
Hsp92I GRCGYC 2 cut(s) 72, 82
Hsp92II CATG 3 cut(s) 327, 544, 563
HspAI GCGC 2 cut(s) 5, 72
KasI GGCGCC 1 cut(s) 71
LmnI GCTCC 2 cut(s) 263, 314
Lsp1109I GCAGC 8 cut(s) 221, 415, 424, 443, 446, 577, 580, 583
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 2 cut(s) 217, 483
MboII GAAGA 8 cut(s) 52, 176, 179, 182, 271, 274, 277, 283
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 1 cut(s) 377
MluNI TGGCCA 1 cut(s) 26
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 7 cut(s) 46, 50, 56, 271, 313, 500, 523
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 1 cut(s) 355
MscI TGGCCA 1 cut(s) 26
MseI TTAA 2 cut(s) 210, 381
MslI CAYNNNNRTG 1 cut(s) 525
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 3 cut(s) 255, 336, 504
MspR9I CCNGG 3 cut(s) 223, 298, 337
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 223, 298
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 8 cut(s) 71, 243, 341, 350, 434, 437, 565, 571
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 337
NlaIII CATG 3 cut(s) 327, 544, 563
NlaIV GGNNCC 3 cut(s) 73, 145, 259
NmuCI GTSAC 2 cut(s) 217, 483
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 2 cut(s) 327, 544
PaeI GCATGC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 588
PfoI TCCNGGA 1 cut(s) 296
PluTI GGCGCC 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 221, 296
PspGI CCWGG 2 cut(s) 221, 296
PspN4I GGNNCC 3 cut(s) 73, 145, 259
PspPI GGNCC 1 cut(s) 532
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 437
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 2 cut(s) 116, 476
RsaNI GTAC 2 cut(s) 115, 475
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 2 cut(s) 210, 381
Sau96I GGNCC 1 cut(s) 532
ScrFI CCNGG 3 cut(s) 223, 298, 337
SexAI ACCWGGT 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 429
SfoI GGCGCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 525
SphI GCATGC 1 cut(s) 327
Sse9I AATT 1 cut(s) 377
SsiI CCGC 2 cut(s) 174, 237
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 3 cut(s) 221, 296, 335
StyI CCWWGG 1 cut(s) 535
TaaI ACNGT 5 cut(s) 134, 160, 205, 277, 450
TaqI TCGA 1 cut(s) 591
TasI AATT 1 cut(s) 377
TatI WGTACW 1 cut(s) 474
TauI GCSGC 2 cut(s) 176, 240
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 2 cut(s) 210, 381
Tru9I TTAA 2 cut(s) 210, 381
TscAI CASTG 2 cut(s) 453, 589
TseFI GTSAC 2 cut(s) 217, 483
TseI GCWGC 8 cut(s) 234, 428, 431, 434, 437, 565, 568, 571
Tsp45I GTSAC 2 cut(s) 217, 483
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 453, 589
XapI RAATTY 1 cut(s) 377
XceI RCATGY 2 cut(s) 327, 544
XspI CTAG 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.