MD11G1172100.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007471
Physical Location & Seq
Reverse (-)
18996740 .. 18997290
551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1172100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGCAGTCTCGATACCCATAGTCGCCATCGTTACCTCCCTCCATCTCATCGCCTTCATCTTCGCCGTCGGCACCGAACGACGCCGTAGCACGGCAAAGATAGTGCCCGATGAGTACGACGAGCGTACCTACTGCGTGTACGGCACGGATGCCTCCACGGTGTACGGACTAGCGGCGTTTGGGCTGCTTGCCGTCAGCCAGACGGTGCTTAACGTCGTCACCAGGTGTCTCTGCTGCGGCAAAGGTCTAGTCACTGGCTCCTCCACCACTTGGACCGTCTTCTTCTTCGTCTTCTCCTGGACAACCTTTTTGGGAGCGGAGGCGTGCTTGTTGGCTGGGTCGGCAAAGAATGCATACCACACCAAGTACCGGGGAATCTTCAAAGTAGATGACTTGTCCTGTGCTACTCTGCGCAAGGGCATGTTTGCCGCCGGCACTGTTCTTACGCTGTTGTCACTGGCAGGATCAAGGGCGAGGTCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

162

Amino Acids

17.27

Weight (kDa)

8.98

Isoelectric Point (pI)

21.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 59 - 153 8.7e-27 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 317
AciI CCGC 4 cut(s) 173, 237, 317, 429
AclWI GGATC 1 cut(s) 472
AcyI GRCGYC 1 cut(s) 82
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 5 cut(s) 116, 127, 140, 164, 368
AfiI CCNNNNNNNGG 3 cut(s) 91, 270, 369
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 2 cut(s) 221, 296
Alw26I GTCTC 2 cut(s) 13, 233
AlwI GGATC 1 cut(s) 472
ApeKI GCWGC 2 cut(s) 184, 234
ArsI GACNNNNNNTTYG 4 cut(s) 234, 266, 292, 324
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 211
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 108
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 2 cut(s) 271, 283
BbvI GCAGC 2 cut(s) 171, 221
BccI CCATC 2 cut(s) 35, 51
BceAI ACGGC 5 cut(s) 50, 69, 108, 157, 176
BciT130I CCWGG 2 cut(s) 223, 298
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 2 cut(s) 13, 233
BfaI CTAG 2 cut(s) 170, 248
BisI GCNGC 5 cut(s) 174, 185, 235, 238, 429
BlsI GCNGC 5 cut(s) 175, 186, 236, 239, 430
Bme1390I CCNGG 3 cut(s) 223, 298, 371
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 2 cut(s) 73, 259
BmrFI CCNGG 3 cut(s) 223, 298, 371
BmsI GCATC 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 271, 283
BpuMI CCSGG 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 156, 370
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 270, 369
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 259, 462
BseBI CCWGG 2 cut(s) 223, 298
BseDI CCNNGG 2 cut(s) 156, 370
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BseNI ACTGG 2 cut(s) 259, 462
BseRI GAGGAG 1 cut(s) 250
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 2 cut(s) 171, 221
BseYI CCCAGC 1 cut(s) 335
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 2 cut(s) 370, 432
BslI CCNNNNNNNGG 3 cut(s) 91, 270, 369
BsmAI GTCTC 2 cut(s) 13, 233
BsmI GAATGC 1 cut(s) 355
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 464
BspACI CCGC 4 cut(s) 173, 237, 317, 429
BspLI GGNNCC 2 cut(s) 73, 259
BspPI GGATC 1 cut(s) 472
BspT107I GGYRCC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 259, 462
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 156, 370
BssMI GATC 1 cut(s) 464
BssNI GRCGYC 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 223, 298
Bst4CI ACNGT 4 cut(s) 160, 205, 277, 439
BstACI GRCGYC 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 3 cut(s) 189, 325, 433
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 1 cut(s) 467
BstMAI GTCTC 2 cut(s) 13, 233
BstMBI GATC 1 cut(s) 464
BstMWI GCNNNNNNNGC 3 cut(s) 141, 341, 350
BstNI CCWGG 2 cut(s) 223, 298
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 221, 296, 369
BstSLI GKGCMC 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 171, 221
BstV2I GAAGAC 2 cut(s) 271, 283
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 3 cut(s) 252, 435, 455
Cac8I GCNNGC 3 cut(s) 189, 325, 433
CfoI GCGC 1 cut(s) 414
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 273
CseI GACGC 1 cut(s) 90
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 5 cut(s) 115, 126, 139, 163, 367
CviAII CATG 1 cut(s) 421
CviJI RGCY 4 cut(s) 184, 198, 258, 335
CviKI_1 RGCY 4 cut(s) 184, 198, 258, 335
CviQI GTAC 5 cut(s) 115, 126, 139, 163, 367
DpnI GATC 1 cut(s) 466
DpnII GATC 1 cut(s) 464
DraIII CACNNNGTG 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 221, 296
EcoT22I ATGCAT 1 cut(s) 355
FaeI CATG 1 cut(s) 424
FaiI YATR 3 cut(s) 20, 355, 422
FatI CATG 1 cut(s) 420
Fnu4HI GCNGC 5 cut(s) 174, 185, 235, 238, 429
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 174, 185, 235, 238, 429
FspBI CTAG 2 cut(s) 170, 248
FspI TGCGCA 1 cut(s) 413
GlaI GCGC 1 cut(s) 413
GluI GCNGC 5 cut(s) 174, 185, 235, 238, 429
GsaI CCCAGC 1 cut(s) 339
HapII CCGG 2 cut(s) 370, 432
HgaI GACGC 1 cut(s) 90
HhaI GCGC 1 cut(s) 414
Hin1I GRCGYC 1 cut(s) 82
Hin1II CATG 1 cut(s) 424
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HinfI GANTC 1 cut(s) 375
HpaII CCGG 2 cut(s) 370, 432
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 2 cut(s) 139, 163
Hpy188I TCNGA 1 cut(s) 481
Hpy188III TCNNGA 1 cut(s) 10
Hpy8I GTNNAC 2 cut(s) 139, 163
Hpy99I CGWCG 4 cut(s) 71, 84, 122, 218
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 4 cut(s) 160, 205, 277, 439
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 341, 350
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 82
Hsp92II CATG 1 cut(s) 424
HspAI GCGC 1 cut(s) 412
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Kzo9I GATC 1 cut(s) 464
LmnI GCTCC 2 cut(s) 263, 314
Lsp1109I GCAGC 2 cut(s) 171, 221
LweI GCATC 1 cut(s) 139
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 2 cut(s) 170, 248
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 4 cut(s) 31, 217, 250, 453
MalI GATC 1 cut(s) 466
MbiI CCGCTC 1 cut(s) 317
MboI GATC 1 cut(s) 464
MboII GAAGA 6 cut(s) 52, 271, 274, 277, 283, 370
MhlI GDGCHC 1 cut(s) 108
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 6 cut(s) 46, 50, 163, 271, 313, 468
Mph1103I ATGCAT 1 cut(s) 355
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 1 cut(s) 210
MspI CCGG 2 cut(s) 370, 432
MspR9I CCNGG 3 cut(s) 223, 298, 371
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 2 cut(s) 223, 298
MwoI GCNNNNNNNGC 3 cut(s) 141, 341, 350
NaeI GCCGGC 1 cut(s) 433
NciI CCSGG 1 cut(s) 371
NdeII GATC 1 cut(s) 464
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 2 cut(s) 73, 259
NmuCI GTSAC 3 cut(s) 217, 250, 453
NsbI TGCGCA 1 cut(s) 413
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 1 cut(s) 424
PctI GAATGC 1 cut(s) 355
PdiI GCCGGC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 296
PkrI GCNGC 5 cut(s) 175, 186, 236, 239, 430
Psp6I CCWGG 2 cut(s) 221, 296
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 221, 296
PspN4I GGNNCC 2 cut(s) 73, 259
PspPI GGNCC 1 cut(s) 273
RsaI GTAC 5 cut(s) 116, 127, 140, 164, 368
RsaNI GTAC 5 cut(s) 115, 126, 139, 163, 367
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 5 cut(s) 174, 185, 235, 238, 429
Sau3AI GATC 1 cut(s) 464
Sau96I GGNCC 1 cut(s) 273
ScrFI CCNGG 3 cut(s) 223, 298, 371
SduI GDGCHC 1 cut(s) 108
SetI ASST 7 cut(s) 38, 131, 216, 227, 247, 308, 479
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 1 cut(s) 139
SinI GGWCC 1 cut(s) 273
SsiI CCGC 4 cut(s) 173, 237, 317, 429
SspMI CTAG 2 cut(s) 170, 248
StyD4I CCNGG 3 cut(s) 221, 296, 369
TaaI ACNGT 4 cut(s) 160, 205, 277, 439
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 11
TauI GCSGC 3 cut(s) 176, 240, 431
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 3 cut(s) 259, 442, 462
TseFI GTSAC 3 cut(s) 217, 250, 453
TseI GCWGC 2 cut(s) 184, 234
Tsp45I GTSAC 3 cut(s) 217, 250, 453
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 2 cut(s) 161, 180
TspRI CASTG 3 cut(s) 259, 442, 462
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 273
XceI RCATGY 1 cut(s) 424
XspI CTAG 2 cut(s) 170, 248
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.