AT1G68890

Protein PHYLLO, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006265
Physical Location & Seq
Forward (+)
25896854 .. 25906714
9861 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68890.2

Sequence Viewer

Length: 5148 bp
ATGCGATCTTCGTTTCTAGTCTCGAATCCTCCATTTCTACCTTCTCTAATCCCTCGTTACAGTTCTAGAAAATCCATCAGACGTAGCAGAGAACGCTTCTCCTTCCCTGAGTCTCTTCGTGTCTCTCTTCTTCATGGAATCCGAAGAAACATTGAGGTCGCTCAAGGAGTTCAATTCGATGGTCCGATTATGGATAGAGACGTGAATTTGGATGATGATTTAGTGGTTCAAGTGTGTGTGACTCGTACATTGCCTCCAGCTTTGACTCTTGAGCTTGGTCTCGAGAGTCTCAAGGAAGCTATTGACGAATTGAAGACTAATCCTCCTAAGTCATCTAGTGGAGTCTTACGATTTCAAGTGGCTGTGCCTCCAAGGGCTAAAGCTTTGTTCTGGTTTTGCTCTCAACCAACGACTTCCGATGTGTTTCCTGTGTTTTTCCTCTCCAAGGATACTGTTGAGCCTTCTTATAAATCACTTTATGTAAAGGAACCTCATGGGGTTTTTGGGATTGGGAATGCATTCGCTTTTGTTCATTCATCTTCAGTTGACTCCAATGGACATAGCATGATCAAAACATTCCTCTCAGATGAATCAGCTATGGTGACGGCCTATGGTTTTCCAGATATTGAATTCAACAAATACTCTACTGTAAATAGCAAAGATGGCTCTTCTTACTTTTTCGTTCCTCAGATTGAGTTAGATGAGCACGAGGAGGTCTCTATATTAGCAGTTACATTGGCATGGAATGAATCTCTCTCTTACACAGTTGAGCAAACAATTAGTTCATATGAGAAATCAATTTTTCAGGTTTCTTCTCATTTCTGCCCAAATGTAGAGGATCACTGGTTCAAACATCTAAAAAGTTCTTTAGCGAAGCTTAGTGTTGAAGAGATACATCCACTTGAGATGGAGCATATGGGCTTTTTCACCTTTTCTGGGAGAGATCAGGCTGACGTCAAGGAACTGAAAAGTATACAATCATCATGTCAGTTCCATTGCAAGCTTTCACCTGACGTTGTTTTCTCAAACAACATGCTGAATCGAGAGACTGAAGTGAGCAACTTCTTGAGAGATGAAGCTAACATCAATGCTGTATGGGCATCAGCTATAATTGAAGAATGCACTCGTCTTGGTTTGACGTACTTTTGTGTAGCCCCTGGATCAAGGTCTTCCCATCTTGCTATCGCTGCTGCCAACCATCCCCTTACAACGTGTCTTGCATGCTTTGATGAACGATCTCTTGCGTTTCACGCTATTGGTTATGCTAAAGGATCCCTCAAACCAGCAGTCATTATAACATCATCAGGAACTGCCGTTTCAAATCTTCTTCCAGCGGTTGTTGAAGCCAGTGAGGATTTCTTACCACTGCTACTACTTACCGCAGATCGTCCTCCTGAACTTCAGGGAGTTGGCGCAAATCAAGCAATAAATCAAATAAACCACTTCGGTTCGTTCGTCAGATTCTTCTTCAATCTTCCTCCTCCAACCGATCTTATTCCAGTCCGAATGGTCCTTACGACTGTAGACTCCGCTCTACATTGGGCAACAGGATCTGCTTGTGGACCAGTACATCTCAATTGTCCTTTTAGAGACCCACTTGACGGTAGTCCAACAAATTGGTCATCTAACTGCTTAAATGGATTAGACATGTGGATGTCTAATGCTGAGCCATTCACAAAATATTTTCAAGTACAAAGCCATAAAAGCGATGGTGTAACAACTGGCCAAATTACTGAGATCTTACAAGTAATCAAAGAGGCTAAGAAGGGTCTTCTTCTTATTGGTGCAATCCATACAGAGGATGAAATTTGGGCTTCGCTTCTGTTGGCGAAAGAGCTGATGTGGCCTGTAGTTGCAGATGTCTTGTCTGGTGTACGGCTGCGCAAGCTTTTTAAACCTTTTGTTGAGAAGTTGACGCATGTTTTTGTTGATCATCTTGATCATGCCTTATTTTCAGACTCTGTCAGGAATTTGATAGAGTTTGATGTTGTTATCCAGGTTGGAAGTCGGATAACAAGTAAAAGAGTTTCTCAGATGCTAGAGAAATGCTTTCCATTTGCATACATTTTGGTTGATAAGCATCCATGCCGACATGACCCATCACACCTGGTCACTCACAGGGTCCAAAGCAACATTGTTCAGTTTGCTAATTGTGTGCTTAAATCTCGATTTCCATGGAGGAGAAGCAAGTTGCATGGTCATCTACAGGCATTGGATGGCGCTATTGCCCGGGAAATGTCATTTCAAATATCTGCTGAAAGCTCCCTGACTGAACCTTACGTTGCACATATGCTTTCTAAAGCACTAACTTCTAAATCTGCTCTTTTCATCGGGAATAGTATGCCAATAAGGGATGTGGATATGTATGGATGTAGTTCAGAAAATTCTTCTCATGTGGTAGATATGATGTTGAGTGCAGAACTACCATGTCAATGGATACAAGTAACTGGAAATAGAGGAGCTAGTGGCATTGATGGGTTGCTCAGCTCGGCCACTGGCTTTGCTGTAGGATGCAAGAAGAGAGTGGTCTGTGTGGTTGGGGACATCTCTTTCCTTCATGATACAAATGGATTGGCGATTTTGAAGCAGAGGATTGCAAGGAAACCAATGACAATTCTTGTGATAAACAACCGTGGAGGTGGAATCTTCCGACTTCTTCCTATTGCAAAGAAAACAGAGCCTAGCGTGTTAAATCAATATTTCTATACAGCACATGACATTTCCATTGAAAACTTGTGTTTGGCACATGGTGTGAGGTATGTTCACGTTGGGACGAAAAGTGAACTTGAGGATGCTCTATTTGTACCAAGCGTGGAGGAGATGGATTGCATTGTGGAGGTTGAAAGCTCTATAAACGCTAACGCGATTGTTCATAGCACTTTGGAGAGGTTTGCACGCCAAGCCGCAGAAAATTCCCTGGGCATTGTTTCTGCCAGTTCTTTTCTTCATCCGATGATCAAAAATGTACTTCTTTGCCAAGTCTCTGGAATACAATATTCGCAGTACAGAGTCAAACTGTGTGACAGACCTACCATATGTTCTGATGAATTCTCTCAATTCCATCGAGAAGGGTTCATACTCTCCCTGACTTTGGAGGATGGAAGCATTGGCTATGGAGAGGTTGCACCTCTGAACAGTAATGTAGAGAACTTAATGGATGTTGAGGGGCAGCTTCAGTTAGTTCTTCACCTCATGAATGAAGCTAAATTTAGTTACATGCTTCCTTTGTTAAATGGCTCGATTTCTTCCTGGATTTGGAGTGAACTTGGAATTACTGCATCATCGATTTTCCCTAGTGTCAGATGTGGTCTGGAAATGGCTCTTCTAAACGCAATGGCAGTAAGACATGATTCTAGTTTGTTGGGGATACTTCATTATCAGAAAGAAGAAAATGGTTCTGCTCAGCCACACTCTGTTCAAATATGTGCCCTTCTTGATTCTGAAGGTACTCCACTGGAGGTCGCATACGTTGCTAGAAAACTTGTTCAAGAAGGGTTCAGTGCTATTAAACTTAAAGTTGGTCGTCGAGTGAGCTCTGTTCAAGATGCTTTAGTTATGCAAGAAGTAAGGAGAGCCGTTGGCGTTCAAATTGAACTCCGTGCAGATGCAAATTGTCGTTGGACTTTTGAAGAGGCTAGAGAGTTTGGTTTATTGGTGAACAGCTGTAATTTAAAATATATTGAGGAACCTGTCCAGAATAAAGATGACCTTATAAGGTTTCATGAAGAAACTGGATTACCAGTGGCACTTGATGAGACTCTTGATGATTTTGAGGAATGTCCTCTGCGTATGCTTACCAAATATACGCACCCTGGAATAGTTGCTGTTGTTATTAAACCAAGTGTTGTGGGAGGGTTTGAGAATGCAGCACTGATTGCTCGGTGGGCACAGCAGCATGGAAAGATGGCTGTTATAAGTGCTGCATATGAAAGTGGCCTAGGTTTATCAGCATATATTTTATTTGCATCATATTTGGAGATGGAGAACGTGAAAGCATCCACAGAGCAGAAGCAAGGAACGCCCCCTTCTGTAGCCCATGGTCTTGGAACTTACCGGTGGCTTAGTGAAGACGTAATGATGAATACATTAGGGATATTTCGTAGCCCTTACAGTGGATTTGTTGAAGGATTTATTGCTGATGCTAGTAGAAATCTAAAGGATGTTAAGATAAACAACGATGTTATTGTTAGAACCAGTAAAGGAATTCCTGTTCGGAGGTATGAACTGAGGGTGGATGTAGATGGTTTCTCTCATTTCATAAGAGTCCATGACGTTGGGGAGAATGCAGAAGGAAGTGTAGCTTTGTTTCTTCATGGATTTCTTGGAACTGGTGAAGAATGGATCCCCATCATGACGGGTATCTCAGGATCTGCGAGATGCATTTCAGTCGATATTCCTGGTCACGGAAGGTCAAGGGTACAAAGTCATGCTAGTGAGACCCAGACGTCCCCAACTTTCTCAATGGAGATGATAGCGGAGGCATTGTATAAGTTGATTGAGCAAATTACTCCTGGGAAAGTAACCATAGTTGGATATTCCATGGGAGCAAGGATAGCACTGTACATGGCTTTGAGGTTTAGCAACAAGATCGAAGGAGCTGTCGTGGTATCAGGGAGCCCGGGACTCAAGGATCCAGTAGCACGGAAAATTCGAAGTGCTACAGATGATTCCAAAGCACGAATGATGGTTGACAATGGACTATATATCTTTATCGAGAATTGGTACAATGGAGGCTTGTGGAAGAGCTTGAGAAATCATCCCCATTTTAGTAAAATTGCTGCAAGCCGCTTGCTACATGGTGATGTTCCTAGTGTTGCAAAGCTCCTGTCTGATCTAAGCAGTGGGAGACAACCGTCATTGTGGGAAGAGTTGGAGGATTGTGATACAAATATCTCGCTCGTATTCGGGGAGAAAGATGTCAAATACAAGCAAATCGCTACTAGAATGTACCGCGAGATGAGTAAAAGCAAGAAGAGCGTAAACAATATCATCGAGATAGTTGAAATTCCAGAGGCTGGTCACGCTGTACATCTCGAGAGCCCTCTTCGCGTGATTCTCGCCCTCAGAAAATTCTTAACAAGAGTTCACAACAGTTCTACAGAGACAGAGCTTTCTCAGAAGCTCTTGTTAGCACTAAAAGAAATGTAA

Protein Analysis

1715

Amino Acids

190.18

Weight (kDa)

6.13

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 23 - 135 8.9e-17 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 200 - 433 1.5e-21 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 478 - 589 3.1e-09 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 813 - 981 1.9e-23 Enolase C-terminal domain-like
Ndr PF03096 1075 - 1208 9.7e-06 Ndr family
Abhydrolase_1 PF00561 1091 - 1202 1.5e-20 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 1091 - 1340 6.4e-17 Alpha/beta hydrolase family
Hydrolase_4 PF12146 1117 - 1335 1.1e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 468, 1295, 3713, 3914
AasI GACNNNNNNGTC 1 cut(s) 4444
AatII GACGTC 2 cut(s) 957, 4448
Acc16I TGCGCA 1 cut(s) 1883
AccB7I CCANNNNNTGG 1 cut(s) 5015
AccBSI CCGCTC 1 cut(s) 1532
AccI GTMKAC 2 cut(s) 973, 1524
AccII CGCG 3 cut(s) 2864, 4953, 5049
AciI CCGC 7 cut(s) 1334, 1380, 1530, 2904, 4475, 4786, 4951
AcoI YGGCCR 2 cut(s) 1723, 2490
AcuI CTGAAG 5 cut(s) 525, 1071, 1385, 3158, 3462
AcyI GRCGYC 2 cut(s) 954, 4445
AdeI CACNNNGTG 1 cut(s) 2750
AfiI CCNNNNNNNGG 9 cut(s) 445, 936, 1601, 1798, 3091, 3255, 4112, 4403, 5015
AflIII ACRYGT 2 cut(s) 1211, 1647
AgeI ACCGGT 1 cut(s) 4053
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 8 cut(s) 1156, 1995, 2106, 2916, 3248, 3811, 4396, 4510
AloI GAACNNNNNNTCC 4 cut(s) 830, 862, 4194, 4226
Alw21I GWGCWC 2 cut(s) 708, 3536
AlwNI CAGNNNCTG 5 cut(s) 1310, 1553, 1961, 4370, 5015
Ama87I CYCGRG 4 cut(s) 281, 2229, 4618, 5033
AoxI GGCC 5 cut(s) 606, 1723, 1844, 2490, 3934
ApeKI GCWGC 8 cut(s) 1187, 1190, 1879, 3169, 3866, 3892, 3920, 4778
ArsI GACNNNNNNTTYG 6 cut(s) 1272, 1304, 4584, 4616, 4811, 4843
AsiGI ACCGGT 1 cut(s) 4053
Asp700I GAANNNNTTC 1 cut(s) 518
AspA2I CCTAGG 1 cut(s) 3937
AspLEI GCGC 3 cut(s) 1415, 1884, 2222
AspS9I GGNCC 4 cut(s) 182, 1510, 1562, 2122
AsuC2I CCSGG 4 cut(s) 2230, 2231, 4619, 4620
AsuHPI GGTGA 7 cut(s) 613, 919, 999, 3179, 3667, 4343, 4811
AsuII TTCGAA 1 cut(s) 4651
AvaI CYCGRG 4 cut(s) 281, 2229, 4618, 5033
AvaII GGWCC 4 cut(s) 182, 1510, 1562, 2122
AvrII CCTAGG 1 cut(s) 3937
BaeGI GKGCMC 2 cut(s) 3430, 3889
BalI TGGCCA 1 cut(s) 1725
BamHI GGATCC 3 cut(s) 1271, 4341, 4630
BanII GRGCYC 3 cut(s) 3536, 4619, 5042
BarI GAAGNNNNNNTAC 2 cut(s) 3060, 3092
BauI CACGAG 1 cut(s) 707
BbsI GAAGAC 4 cut(s) 320, 1161, 1763, 4074
Bbv12I GWGCWC 2 cut(s) 708, 3536
BbvI GCAGC 8 cut(s) 1174, 1177, 1866, 3181, 3878, 3904, 3907, 4765
BceAI ACGGC 4 cut(s) 621, 1298, 1892, 3560
BcgI CGANNNNNNTGC 4 cut(s) 4369, 4403, 4570, 4604
BciT130I CCWGG 8 cut(s) 1158, 1997, 2108, 2918, 3250, 3813, 4398, 4512
BciVI GTATCC 3 cut(s) 442, 2430, 3360
BclI TGATCA 4 cut(s) 567, 1930, 1939, 2955
BcnI CCSGG 4 cut(s) 2230, 2231, 4619, 4620
BfmI CTRYAG 7 cut(s) 1521, 1848, 2204, 2505, 4029, 4659, 5097
BfoI RGCGCY 1 cut(s) 2223
BfuI GTATCC 3 cut(s) 442, 2430, 3360
BglII AGATCT 1 cut(s) 1737
BlnI CCTAGG 1 cut(s) 3937
BlpI GCTNAGC 3 cut(s) 1665, 2483, 3402
Bme18I GGWCC 4 cut(s) 182, 1510, 1562, 2122
BmeT110I CYCGRG 4 cut(s) 281, 2229, 4618, 5033
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 4 cut(s) 182, 1510, 1562, 2122
BmiI GGNNCC 7 cut(s) 489, 1273, 2123, 3687, 4343, 4616, 4632
BoxI GACNNNNGTC 2 cut(s) 1605, 4852
BpiI GAAGAC 4 cut(s) 320, 1161, 1763, 4074
BplI GAGNNNNNCTC 2 cut(s) 701, 733
BpmI CTGGAG 2 cut(s) 240, 3476
Bpu1102I GCTNAGC 3 cut(s) 1665, 2483, 3402
Bpu14I TTCGAA 1 cut(s) 4651
BpuEI CTTGAG 8 cut(s) 147, 275, 290, 923, 1087, 2807, 4610, 4768
BpuMI CCSGG 4 cut(s) 2230, 2231, 4619, 4620
Bsa29I ATCGAT 1 cut(s) 3284
BsaBI GATNNNNATC 2 cut(s) 2079, 4346
BsaHI GRCGYC 2 cut(s) 954, 4445
BsaI GGTCTC 4 cut(s) 284, 721, 1584, 4430
BsaWI WCCGGW 1 cut(s) 4053
BsaXI ACNNNNNCTCC 8 cut(s) 238, 268, 307, 337, 4469, 4499, 4587, 4617
Bsc4I CCNNNNNNNGG 9 cut(s) 445, 936, 1601, 1798, 3091, 3255, 4112, 4403, 5015
Bse118I RCCGGY 1 cut(s) 4053
Bse3DI GCAATG 3 cut(s) 248, 994, 3339
Bse8I GATNNNNATC 2 cut(s) 2079, 4346
BseBI CCWGG 8 cut(s) 1158, 1997, 2108, 2918, 3250, 3813, 4398, 4512
BseCI ATCGAT 1 cut(s) 3284
BseJI GATNNNNATC 2 cut(s) 2079, 4346
BseLI CCNNNNNNNGG 9 cut(s) 445, 936, 1601, 1798, 3091, 3255, 4112, 4403, 5015
BseMI GCAATG 3 cut(s) 248, 994, 3339
BseRI GAGGAG 5 cut(s) 725, 1470, 2194, 2472, 2831
BseSI GKGCMC 2 cut(s) 3430, 3889
BseXI GCAGC 8 cut(s) 1174, 1177, 1866, 3181, 3878, 3904, 3907, 4765
BsgI GTGCAG 2 cut(s) 2436, 3621
Bsh1236I CGCG 3 cut(s) 2864, 4953, 5049
BshFI GGCC 5 cut(s) 608, 1725, 1846, 2492, 3936
BshTI ACCGGT 1 cut(s) 4053
BshVI ATCGAT 1 cut(s) 3284
BsiHKAI GWGCWC 2 cut(s) 708, 3536
BsiHKCI CYCGRG 4 cut(s) 281, 2229, 4618, 5033
BsiSI CCGG 3 cut(s) 2230, 4054, 4619
BslFI GGGAC 4 cut(s) 2555, 2785, 4432, 4635
BslI CCNNNNNNNGG 9 cut(s) 445, 936, 1601, 1798, 3091, 3255, 4112, 4403, 5015
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 4 cut(s) 2555, 2785, 4432, 4635
BsmI GAATGC 5 cut(s) 518, 520, 1124, 3868, 4288
BsnI GGCC 5 cut(s) 608, 1725, 1846, 2492, 3936
Bso31I GGTCTC 4 cut(s) 284, 721, 1584, 4430
BsoBI CYCGRG 4 cut(s) 281, 2229, 4618, 5033
Bsp119I TTCGAA 1 cut(s) 4651
Bsp1286I GDGCHC 6 cut(s) 708, 3430, 3536, 3889, 4619, 5042
Bsp1407I TGTACA 2 cut(s) 4560, 5026
Bsp1720I GCTNAGC 3 cut(s) 1665, 2483, 3402
Bsp19I CCATGG 3 cut(s) 2174, 4036, 4539
BspACI CCGC 7 cut(s) 1334, 1380, 1530, 2904, 4475, 4786, 4951
BspANI GGCC 5 cut(s) 608, 1725, 1846, 2492, 3936
BspDI ATCGAT 1 cut(s) 3284
BspFNI CGCG 3 cut(s) 2864, 4953, 5049
BspHI TCATGA 4 cut(s) 2557, 3192, 3721, 4350
BspLI GGNNCC 7 cut(s) 489, 1273, 2123, 3687, 4343, 4616, 4632
BspQI GCTCTTC 4 cut(s) 673, 3327, 4736, 4967
BspT104I TTCGAA 1 cut(s) 4651
BspTNI GGTCTC 4 cut(s) 284, 721, 1584, 4430
BsrBI CCGCTC 1 cut(s) 1532
BsrDI GCAATG 3 cut(s) 248, 994, 3339
BsrFI RCCGGY 1 cut(s) 4053
BsrGI TGTACA 2 cut(s) 4560, 5026
BssAI RCCGGY 1 cut(s) 4053
BssNAI GTATAC 1 cut(s) 974
BssNI GRCGYC 2 cut(s) 954, 4445
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 6 cut(s) 371, 444, 2174, 3937, 4036, 4539
Bst1107I GTATAC 1 cut(s) 974
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 8 cut(s) 1158, 1997, 2108, 2918, 3250, 3813, 4398, 4512
BstACI GRCGYC 2 cut(s) 954, 4445
BstAPI GCANNNNNTGC 2 cut(s) 3470, 3875
BstAUI TGTACA 2 cut(s) 4560, 5026
BstBI TTCGAA 1 cut(s) 4651
BstC8I GCNNGC 6 cut(s) 1001, 1222, 1886, 2896, 4783, 4790
BstDSI CCRYGG 4 cut(s) 2174, 2632, 4036, 4539
BstENI CCTNNNNNAGG 1 cut(s) 443
BstFNI CGCG 3 cut(s) 2864, 4953, 5049
BstH2I RGCGCY 1 cut(s) 2223
BstHHI GCGC 3 cut(s) 1415, 1884, 2222
BstNI CCWGG 8 cut(s) 1158, 1997, 2108, 2918, 3250, 3813, 4398, 4512
BstNSI RCATGY 5 cut(s) 1036, 1224, 1651, 1922, 3220
BstPAI GACNNNNGTC 2 cut(s) 1605, 4852
BstSFI CTRYAG 7 cut(s) 1521, 1848, 2204, 2505, 4029, 4659, 5097
BstSLI GKGCMC 2 cut(s) 3430, 3889
BstUI CGCG 3 cut(s) 2864, 4953, 5049
BstV1I GCAGC 8 cut(s) 1174, 1177, 1866, 3181, 3878, 3904, 3907, 4765
BstV2I GAAGAC 4 cut(s) 320, 1161, 1763, 4074
BstX2I RGATCY 6 cut(s) 1271, 1550, 1737, 4341, 4367, 4630
BstXI CCANNNNNNTGG 5 cut(s) 1617, 2433, 2984, 4043, 4274
BstYI RGATCY 6 cut(s) 1271, 1550, 1737, 4341, 4367, 4630
BstZ17I GTATAC 1 cut(s) 974
Bsu15I ATCGAT 1 cut(s) 3284
BsuI GTATCC 3 cut(s) 442, 2430, 3360
BsuRI GGCC 5 cut(s) 608, 1725, 1846, 2492, 3936
BsuTUI ATCGAT 1 cut(s) 3284
BtgI CCRYGG 4 cut(s) 2174, 2632, 4036, 4539
BtgZI GCGATG 1 cut(s) 1722
BtrI CACGTC 1 cut(s) 202
BtsI GCAGTG 2 cut(s) 1364, 4846
Cac8I GCNNGC 6 cut(s) 1001, 1222, 1886, 2896, 4783, 4790
CaiI CAGNNNCTG 5 cut(s) 1310, 1553, 1961, 4370, 5015
CciI TCATGA 4 cut(s) 2557, 3192, 3721, 4350
CfoI GCGC 3 cut(s) 1415, 1884, 2222
Cfr10I RCCGGY 1 cut(s) 4053
Cfr13I GGNCC 4 cut(s) 182, 1510, 1562, 2122
Cfr9I CCCGGG 2 cut(s) 2229, 4618
ClaI ATCGAT 1 cut(s) 3284
CseI GACGC 1 cut(s) 1924
CsiI ACCWGGT 1 cut(s) 2106
CspAI ACCGGT 1 cut(s) 4053
CspCI CAANNNNNGTGG 4 cut(s) 2482, 2517, 3828, 3863
DraI TTTAAA 2 cut(s) 1894, 3672
DraIII CACNNNGTG 1 cut(s) 2750
DrdI GACNNNNNNGTC 1 cut(s) 4444
DseDI GACNNNNNNGTC 1 cut(s) 4444
EaeI YGGCCR 2 cut(s) 1723, 2490
Ecl136II GAGCTC 1 cut(s) 3534
Eco130I CCWWGG 6 cut(s) 371, 444, 2174, 3937, 4036, 4539
Eco24I GRGCYC 3 cut(s) 3536, 4619, 5042
Eco31I GGTCTC 4 cut(s) 284, 721, 1584, 4430
Eco47I GGWCC 4 cut(s) 182, 1510, 1562, 2122
Eco53kI GAGCTC 1 cut(s) 3534
Eco57I CTGAAG 5 cut(s) 525, 1071, 1385, 3158, 3462
Eco88I CYCGRG 4 cut(s) 281, 2229, 4618, 5033
EcoICRI GAGCTC 1 cut(s) 3534
EcoNI CCTNNNNNAGG 1 cut(s) 443
EcoRI GAATTC 3 cut(s) 629, 3047, 4203
EcoRII CCWGG 8 cut(s) 1156, 1995, 2106, 2916, 3248, 3811, 4396, 4510
EcoT14I CCWWGG 6 cut(s) 371, 444, 2174, 3937, 4036, 4539
EcoT22I ATGCAT 2 cut(s) 520, 4382
EcoT38I GRGCYC 3 cut(s) 3536, 4619, 5042
ErhI CCWWGG 6 cut(s) 371, 444, 2174, 3937, 4036, 4539
Esp3I CGTCTC 1 cut(s) 192
FalI AAGNNNNNCTT 4 cut(s) 3693, 3725, 4285, 4317
FaqI GGGAC 4 cut(s) 2555, 2785, 4432, 4635
FauNDI CATATG 5 cut(s) 787, 915, 2289, 3035, 3925
FbaI TGATCA 4 cut(s) 567, 1930, 1939, 2955
FblI GTMKAC 2 cut(s) 973, 1524
FriOI GRGCYC 3 cut(s) 3536, 4619, 5042
FspI TGCGCA 1 cut(s) 1883
GlaI GCGC 3 cut(s) 1414, 1883, 2221
GsuI CTGGAG 2 cut(s) 240, 3476
HaeII RGCGCY 1 cut(s) 2223
HaeIII GGCC 5 cut(s) 608, 1725, 1846, 2492, 3936
HapII CCGG 3 cut(s) 2230, 4054, 4619
HgaI GACGC 1 cut(s) 1924
HhaI GCGC 3 cut(s) 1415, 1884, 2222
Hin1I GRCGYC 2 cut(s) 954, 4445
Hin6I GCGC 3 cut(s) 1413, 1882, 2220
HinP1I GCGC 3 cut(s) 1413, 1882, 2220
HincII GTYRAC 3 cut(s) 547, 1914, 4690
HindII GTYRAC 3 cut(s) 547, 1914, 4690
HindIII AAGCTT 4 cut(s) 381, 875, 1001, 1886
HpaII CCGG 3 cut(s) 2230, 4054, 4619
HphI GGTGA 7 cut(s) 613, 919, 999, 3179, 3667, 4343, 4811
Hpy99I CGWCG 1 cut(s) 3528
Hsp92I GRCGYC 2 cut(s) 954, 4445
HspAI GCGC 3 cut(s) 1413, 1882, 2220
Ksp22I TGATCA 4 cut(s) 567, 1930, 1939, 2955
LguI GCTCTTC 4 cut(s) 673, 3327, 4736, 4967
LmnI GCTCC 7 cut(s) 910, 2267, 2459, 4544, 4595, 4614, 4827
Lsp1109I GCAGC 8 cut(s) 1174, 1177, 1866, 3181, 3878, 3904, 3907, 4765
MabI ACCWGGT 1 cut(s) 2106
MbiI CCGCTC 1 cut(s) 1532
MfeI CAATTG 1 cut(s) 1576
MflI RGATCY 6 cut(s) 1271, 1550, 1737, 4341, 4367, 4630
MhlI GDGCHC 6 cut(s) 708, 3430, 3536, 3889, 4619, 5042
MlsI TGGCCA 1 cut(s) 1725
MluNI TGGCCA 1 cut(s) 1725
MmeI TCCRAC 8 cut(s) 1508, 1634, 1981, 1988, 2674, 3599, 4510, 4851
Mox20I TGGCCA 1 cut(s) 1725
Mph1103I ATGCAT 2 cut(s) 520, 4382
MroXI GAANNNNTTC 1 cut(s) 518
MscI TGGCCA 1 cut(s) 1725
MslI CAYNNNNRTG 5 cut(s) 739, 1652, 2431, 4431, 4800
Msp20I TGGCCA 1 cut(s) 1725
MspA1I CMGCKG 2 cut(s) 1334, 3663
MspI CCGG 3 cut(s) 2230, 4054, 4619
MunI CAATTG 1 cut(s) 1576
Mva1269I GAATGC 5 cut(s) 518, 520, 1124, 3868, 4288
MvaI CCWGG 8 cut(s) 1158, 1997, 2108, 2918, 3250, 3813, 4398, 4512
MvnI CGCG 3 cut(s) 2864, 4953, 5049
NciI CCSGG 4 cut(s) 2230, 2231, 4619, 4620
NcoI CCATGG 3 cut(s) 2174, 4036, 4539
NdeI CATATG 5 cut(s) 787, 915, 2289, 3035, 3925
NlaIV GGNNCC 7 cut(s) 489, 1273, 2123, 3687, 4343, 4616, 4632
NmeAIII GCCGAG 1 cut(s) 2468
NmuCI GTSAC 6 cut(s) 238, 601, 2110, 3020, 4400, 5018
NsbI TGCGCA 1 cut(s) 1883
NsiI ATGCAT 2 cut(s) 520, 4382
NspI RCATGY 5 cut(s) 1036, 1224, 1651, 1922, 3220
NspV TTCGAA 1 cut(s) 4651
PaeI GCATGC 1 cut(s) 1224
PaeR7I CTCGAG 2 cut(s) 281, 5033
PagI TCATGA 4 cut(s) 2557, 3192, 3721, 4350
PasI CCCWGGG 1 cut(s) 2917
PciI ACATGT 1 cut(s) 1647
PciSI GCTCTTC 4 cut(s) 673, 3327, 4736, 4967
PcsI WCGNNNNNNNCGW 2 cut(s) 1452, 4648
PctI GAATGC 5 cut(s) 518, 520, 1124, 3868, 4288
PdmI GAANNNNTTC 1 cut(s) 518
PflFI GACNNNGTC 1 cut(s) 1961
PflMI CCANNNNNTGG 1 cut(s) 5015
PfoI TCCNGGA 1 cut(s) 3248
PinAI ACCGGT 1 cut(s) 4053
PscI ACATGT 1 cut(s) 1647
PshAI GACNNNNGTC 2 cut(s) 1605, 4852
PsiI TTATAA 4 cut(s) 468, 1295, 3713, 3914
Psp124BI GAGCTC 1 cut(s) 3536
Psp6I CCWGG 8 cut(s) 1156, 1995, 2106, 2916, 3248, 3811, 4396, 4510
PspGI CCWGG 8 cut(s) 1156, 1995, 2106, 2916, 3248, 3811, 4396, 4510
PspN4I GGNNCC 7 cut(s) 489, 1273, 2123, 3687, 4343, 4616, 4632
PspPI GGNCC 4 cut(s) 182, 1510, 1562, 2122
PstNI CAGNNNCTG 5 cut(s) 1310, 1553, 1961, 4370, 5015
PsuI RGATCY 6 cut(s) 1271, 1550, 1737, 4341, 4367, 4630
PsyI GACNNNGTC 1 cut(s) 1961
PvuII CAGCTG 1 cut(s) 3663
RseI CAYNNNNRTG 5 cut(s) 739, 1652, 2431, 4431, 4800
SacI GAGCTC 1 cut(s) 3536
SapI GCTCTTC 4 cut(s) 673, 3327, 4736, 4967
Sau96I GGNCC 4 cut(s) 182, 1510, 1562, 2122
SduI GDGCHC 6 cut(s) 708, 3430, 3536, 3889, 4619, 5042
SexAI ACCWGGT 1 cut(s) 2106
SfcI CTRYAG 7 cut(s) 1521, 1848, 2204, 2505, 4029, 4659, 5097
Sfr274I CTCGAG 2 cut(s) 281, 5033
SfuI TTCGAA 1 cut(s) 4651
SinI GGWCC 4 cut(s) 182, 1510, 1562, 2122
SlaI CTCGAG 2 cut(s) 281, 5033
SmaI CCCGGG 2 cut(s) 2231, 4620
SmiMI CAYNNNNRTG 5 cut(s) 739, 1652, 2431, 4431, 4800
SphI GCATGC 1 cut(s) 1224
SsiI CCGC 7 cut(s) 1334, 1380, 1530, 2904, 4475, 4786, 4951
SspI AATATT 3 cut(s) 1682, 2699, 2996
SstI GAGCTC 1 cut(s) 3536
StyI CCWWGG 6 cut(s) 371, 444, 2174, 3937, 4036, 4539
TatI WGTACW 6 cut(s) 1567, 1690, 2965, 3003, 4560, 5026
TauI GCSGC 2 cut(s) 2906, 4788
TseFI GTSAC 6 cut(s) 238, 601, 2110, 3020, 4400, 5018
TseI GCWGC 8 cut(s) 1187, 1190, 1879, 3169, 3866, 3892, 3920, 4778
Tsp45I GTSAC 6 cut(s) 238, 601, 2110, 3020, 4400, 5018
TspGWI ACGGA 3 cut(s) 3587, 4419, 4657
TspMI CCCGGG 2 cut(s) 2229, 4618
Tth111I GACNNNGTC 1 cut(s) 1961
Van91I CCANNNNNTGG 1 cut(s) 5015
VpaK11BI GGWCC 4 cut(s) 182, 1510, 1562, 2122
XagI CCTNNNNNAGG 1 cut(s) 443
XbaI TCTAGA 1 cut(s) 65
XceI RCATGY 5 cut(s) 1036, 1224, 1651, 1922, 3220
XcmI CCANNNNNNNNNTGG 1 cut(s) 1706
XhoI CTCGAG 2 cut(s) 281, 5033
XmaI CCCGGG 2 cut(s) 2229, 4618
XmaJI CCTAGG 1 cut(s) 3937
XmiI GTMKAC 2 cut(s) 973, 1524
XmnI GAANNNNTTC 1 cut(s) 518
ZraI GACGTC 2 cut(s) 955, 4446
Zsp2I ATGCAT 2 cut(s) 520, 4382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.