Rh4DG355000

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006265
Physical Location & Seq
Forward (+)
57640795 .. 57652285
11491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG355000.1

Sequence Viewer

Length: 4617 bp
ATGCTACTACTCACAAACTCTCTCTCTTTCCTACACTCCAACACAAGGTCTCTCCCTCACACCAAACCCTCGCCTCCGCCGCCTTTCCCGTTCCTCCACCCAAACTCCCGCCATCTGCGCCTCCCTCCTAACTCCAAGGTCGTTCGAGGCGTTAGATTCGACGGCCCGGCCATCGATATCAGCCAAGTGACGCACACCGACGACGGCGATTTGATAATCGAGACTTGCCTCACGCGGAACTTGACTCCGGCGTTGTCTATTGAGCAAGGGCTTGAGAGTATCAAGGAGGCGGTTGAGGAGTTGAAGCTCCATCCTCCTTCAACTTGTAGTGGAATTCTCAGGTTTCAGGTAGCTGTGCCGCCGAGTGCGAAAGCCTTGAACTGGTTTTGCTGCCAACCGGAGTCGTCTGCGGTCTATCCACTGTTTTTTGTTTCCAAGGAAACTGAAAATCCAAGCTATAAATCATTGTATGTGAATGAAACTCGAGGAGTTTTCGGAATCGGTGCTGCAATTTACTTCACACCTTCTTCTAATTCTTCTTCTTCGATCAAAAGAATTCTATCAAATGAATCAGCTTTTGTAATAGCTTATGGTTTTATGGATATTAATTACGACCAAGAGTCGTCTTATATGAAGCATGAGCCAGGTTCATATTACTTTTTTATTCCTCAGATTGAGTTACATGAGCATGAGGGCGCTTCTGTATTAGCAGCAACTATTGGTTGGAGTGAGTCTTCCCTTTGTACATTTGAGGAAGCTGTTCTTTCTTATGAGTTATGTTTCAACCAGGTTACCTGTCAAGTTTGTTCCACAACAAAGAAATCCCACACCACAAATATAAGATCTACTCTTAGGAAGCTCAATCTTCTGGAGGATGGAATGGTTTCTATGGTACACATGAACATGCTGTTAGCAGGTGGAAAAAACATTTTGTCTGACATCATGGCACTGCTTTTGCAGAAAGAGGCTCCGTCTTCCTCCCAGTTTTGTGTGAGGCTATCACCTACCATGGCTGTTGCTAATAAAATGTTGGATCATGCCAGTAAAATGTGCTTCTCAGTGCAAGATTGTGGCAACATCAACACTGTTTGGGCATCACTTATAGTTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCCCTTGCTGTTGCTGCATCCACTCATCCACTTATTACATGTATTGTATGTTATGATGAACGCTCACTTGCATTTCATGCTGTTGGGTATGCAAGAGGATCTGGCAAACCAGCAGTAGTCATAACATCATCTGGCACTGCAGTTTCAAATCTTCTTCCTGCTGTGGTCGAGGCTAGTCAAGATTTTGTACCACTTATATTGCTCACAGCTGATCGTCCTGCAGAACTTCAGGATGCTGGAGCAAACCAAGCAATCAATCAGGTAAACCATTTTGGCTCTTTTGCACGGTTTTTCTTTAGCCTTCCTGCACCCACAGATCATATTTCAGCTCGCATGGTGCTTACAACACTCGACAATGCTGTACATTGGGCAACCTCTTCACCATGTGGGCCTGTTCATATTAACTGTCCTTTCAGGGAGCCACTAGAAGATAGCCCTAGAAAATGGATGTTGAGTTGTTTAAATGGATTAAACCATTGGATGTCTAATTCTGAACCATTTACCAAGTATATTCAAGTACAACATGCTCATACAAGCAATGATGGTTGTGGCGGAATGTCAGAAATTCTAAATGTAATTAAGGGAACTAACAAGGGTCTTCTACTTATCGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTCTTGGCTAAACACCTTCACTGGCCTGTTATAGCTGACATCTTGTCAGGATTACGATTGAGAAAGCTTTTGACTAGTTTCCCTGAAGTTGACAATGATTTATTGTTTGTTGATCATCTTGACCATGCCCTACTGTCAGATTCTGTCAGTAAATGGATTAACATTGATGTGATTATTCAGATTGGGAGCAGGATTACAAGCAAGCGTATTGCTAAGATGCTAGAGGAATCCTTTCCCTGCTCATATATCATGGTTGACAAGCATCCATTTCGTCACGATCCTTCTCATATTGTAACTCATAGAGTCCACAGTAACATTTTTGAATTTGCTGATTATATCCTTAAAGCTGAATTTCCCCATCTCAGCAAGGAATGGAGCACTTATCTTCAAATGATAAATGCAATGGTTGAAAGGGAGTTGTCATTTCAAATTTCTGCTAGACACTCCTTGACAGAACCTCAAGTCGCATACCTAATTTCAGAAGCACTTTCAGCTGAATCTGCTCTTTTCATTGGGAACAGTATGGCAATACGTGATGCAGATATGTATGGGCGTGGTTGGTCAGAGTATACACGTAGTATTGTTGCCATGAATTATAAACTACCCTGTCAAATGATTCGGGTGGCTGGGAATAGAGGTGCAAGTGGTATTGATGGGTTACTCAGTACAGCTGTTGGCTTTGCTGTAGGATGCAATAAACGAGTACTTTGCGTGATTGGGGATGTTTCCTTCCTCCATGATACAAATGGCTTAGCAATTGTGAACCAGAGGACGTTCCGGAAACCAATGACAATAGTTGTGATTAACAATCATGGTGGTGCAATATTCAGTCTCCTTCCTCTTGCAGATAGAGTCAGACCAAGAATACTAAACCAATATTTCTACACTTCTCACAACATATCAATCCGTGAGCTTTGTGTAGCACATGGTGTGATGCATCTACATGCGACGACAAAGTTGGATTTTGAAGAAGCGTTATTCACATCTCAACATGGAGGGATGGATTGTGTCATTGAAGTTGAGAGCTGCATAGATACCAATGCCAGCTTTCACAGTACTTTAAAAACATTTGCATGCCAAGCAGCAGATCAAGTCATAAAGCCTTCTAGTCAAGACTCGATATTAGATGGCACTTTACTCTGCAGAGTCCATAGAATGGAGTACTCACTGTTTAGAATGCCACTGTGTGCTCCTCATACTATGGTTTCTGTTGATGGCGGTACCACCAGCTTCCACAGGGAAGGGTTTATTTTAACTTTGTATTTTGAAGATGGAAGCTTTGGGCTTGGCGAGGTTTCACCCCTAGACATCTACAAAGAAAACCTTCTAGATGTAGAAGAGCAACTGAGGTTTCTCATCCATATGATGAAAGGATCTCATATCAGTTGCTTCCTTCCTCTGCTAAAGGGCTCGTTTTCATCTTGGATATGGTCCAACCTTGGAATCCTGCCATGTACGCTCTTTCCTAGTGTGAGATGTGGTTTGGAAATGGCAATTCTCAATGCCATAGCAAATAGAAAAGGTTCCAATTTATTAGGAATACTCCATCCCCAGATAGTTGGAGATATATTTAAATCGTCGTCAACAGTTCAGATTTGTGCCCTTGTTGATTCCAGTGGGACACCCACACAAGTAGCTGATGCTATTGCTACACTTGTTGAAGAAGGATTTACTGCAGTAAAGATTAAGGTAGCTCGTTGTGGGAGTCCCTTACAGGATGCTGCAGTTATACAAGAAGTAAGAAAAAAAGTTGGATACCATATTAAAATTCGTGCAGATGCCAATCGGAACTGGACCTATGATGAAGCTATCCAGTTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATCGAGGAACCTGTTCAAATTGAAGAAGATATTATCAAGTTTTGCAAAGAAAGTGGGTTGCCTGTGGCACTGGATGAAACTATTGACAATATTGGAGAACATCCACTTATAAAGCTCTCAAAGTATGCCCATCCAGGAATAGTAGCTGTTGTCATCAAGCCAAGTGTTGTTGGTGGCTTTGAAAATGCAGCAATAATTGCCCAATGGGCCCAGCAGCACCAGAAAATGGCTGTTGTTAGTGCTGCATTTGAAAGCAGCCTAGGTTTGTCAGCATATATTCAGTTTTCCTGTTATCTCAACCAGATGAATTCAGAGATTTGTACAATGATGAACTATCCATTAGCCTCATCCATAGCCCATGGGTTAGGAACTTACCGCTGGCTAAAAGAAGATTTGACGACTACTCCTCTCAAGATAAATCGTAATCCACGTAGTGGGTTCGTAGAAGCATCTGTTGCTGATTCTGATCGAGTTCTGCAGCAATTTCAAATCAATGGAAACATAATTCATAGAAGGGTTACTGGAGAGCAAGTTTGTATGTACCAGTTGCCTTTAGATTCAAAGGGTTTATCTTGCTCCATCAAAATTCAGGAGATTGGACAAGGATATGATGATAATGTATTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATCATGAAGGCCTTATCAGGATGTGGAAGATGCGTTTCTATTGACCTTCCTGGTCATGGTGGAACCAAGATACAAAGTCATGGTGATAAGGATGCTGTACAGGCTTCAGGTTTGTGTGTTGAGGTAGTTGCCGATCTTTTATGTAAAGTGATTGAGCATATTACTCCTGGGAAGGTTACCCTTGTCGGATATTCTATGGGAGCCAGGATCGCCCTTTGCATGGCTCTGAAGCTTACTAAAAAACCCTGGATTAAACGATGGAATGGCAAGAAAAATTCGTAG

Protein Analysis

1538

Amino Acids

169.49

Weight (kDa)

6.32

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 365 - 477 3.2e-22 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 543 - 776 2.4e-23 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 826 - 935 1.5e-06 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 1150 - 1325 4.5e-24 Enolase C-terminal domain-like
Abhydrolase_1 PF00561 1428 - 1523 5.6e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 1430 - 1520 1.2e-10 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2412, 3776
AarI CACCTGC 1 cut(s) 905
Acc36I ACCTGC 1 cut(s) 905
Acc65I GGTACC 1 cut(s) 3035
AccB1I GGYRCC 1 cut(s) 3035
AccB7I CCANNNNNTGG 3 cut(s) 2971, 3892, 4100
AccI GTMKAC 1 cut(s) 2384
AccII CGCG 1 cut(s) 235
AccIII TCCGGA 1 cut(s) 2592
AclWI GGATC 6 cut(s) 1041, 1162, 1273, 2087, 3196, 4550
AcoI YGGCCR 1 cut(s) 168
AcuI CTGAAG 4 cut(s) 1379, 1920, 4425, 4583
AdeI CACNNNGTG 4 cut(s) 1553, 2744, 3002, 4100
AfiI CCNNNNNNNGG 7 cut(s) 45, 381, 2971, 3892, 4100, 4391, 4555
AflIII ACRYGT 2 cut(s) 1205, 2387
AhdI GACNNNNNGTC 2 cut(s) 619, 1858
AhlI ACTAGT 1 cut(s) 1889
AjnI CCWGG 8 cut(s) 643, 786, 1150, 3799, 4384, 4501, 4538, 4580
Alw21I GWGCWC 2 cut(s) 2195, 3007
Alw26I GTCTC 3 cut(s) 54, 215, 2651
AlwI GGATC 6 cut(s) 1041, 1162, 1273, 2087, 3196, 4550
AlwNI CAGNNNCTG 1 cut(s) 1958
Ama87I CYCGRG 1 cut(s) 483
Aor13HI TCCGGA 1 cut(s) 2592
AoxI GGCC 6 cut(s) 163, 168, 1556, 1838, 3873, 4344
ApaI GGGCCC 1 cut(s) 3877
AseI ATTAAT 1 cut(s) 606
Asp700I GAANNNNTTC 6 cut(s) 759, 883, 2046, 3138, 3337, 3678
Asp718I GGTACC 1 cut(s) 3035
AspA2I CCTAGG 1 cut(s) 3925
AspLEI GCGC 2 cut(s) 120, 698
AspS9I GGNCC 6 cut(s) 164, 1556, 3246, 3609, 3873, 3874
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 5 cut(s) 993, 1539, 3105, 4331, 4430
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 2 cut(s) 3246, 3609
AvrII CCTAGG 1 cut(s) 3925
BaeGI GKGCMC 2 cut(s) 3418, 3877
BanI GGYRCC 1 cut(s) 3035
BanII GRGCYC 2 cut(s) 3227, 3877
BbsI GAAGAC 3 cut(s) 726, 966, 1757
Bbv12I GWGCWC 2 cut(s) 2195, 3007
BceAI ACGGC 2 cut(s) 178, 220
BcgI CGANNNNNNTGC 4 cut(s) 2066, 2100, 2505, 2539
BciT130I CCWGG 8 cut(s) 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
BciVI GTATCC 1 cut(s) 3563
BclI TGATCA 1 cut(s) 1927
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 3 cut(s) 54, 215, 2651
BcuI ACTAGT 1 cut(s) 1889
BfmI CTRYAG 8 cut(s) 1305, 1386, 2499, 2956, 3489, 3537, 3659, 4142
BfoI RGCGCY 1 cut(s) 699
BfuAI ACCTGC 1 cut(s) 905
BfuI GTATCC 1 cut(s) 3563
BglI GCCNNNNNGGC 1 cut(s) 3872
BglII AGATCT 1 cut(s) 842
BlnI CCTAGG 1 cut(s) 3925
BlpI GCTNAGC 1 cut(s) 2566
BmcAI AGTACT 3 cut(s) 2520, 2872, 2978
Bme1390I CCNGG 9 cut(s) 167, 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
Bme18I GGWCC 2 cut(s) 3246, 3609
BmeRI GACNNNNNGTC 2 cut(s) 619, 1858
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 6 cut(s) 164, 1556, 3246, 3609, 3873, 3874
BmiI GGNNCC 9 cut(s) 969, 1587, 3037, 3340, 3637, 3675, 3875, 4399, 4537
BmrFI CCNGG 9 cut(s) 167, 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
BmrI ACTGGG 1 cut(s) 976
BmuI ACTGGG 1 cut(s) 976
BpiI GAAGAC 3 cut(s) 726, 966, 1757
BpmI CTGGAG 4 cut(s) 890, 1134, 1425, 4209
Bpu1102I GCTNAGC 1 cut(s) 2566
BpuEI CTTGAG 3 cut(s) 293, 2259, 4061
BpuMI CCSGG 1 cut(s) 167
Bsa29I ATCGAT 1 cut(s) 174
BsaAI YACGTR 3 cut(s) 2348, 2390, 4097
BsaBI GATNNNNATC 5 cut(s) 1159, 3597, 3654, 4131, 4334
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 9 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024, 4502, 4580
BsaWI WCCGGW 2 cut(s) 397, 2592
BsaXI ACNNNNNCTCC 6 cut(s) 89, 119, 4054, 4084, 4182, 4212
Bsc4I CCNNNNNNNGG 7 cut(s) 45, 381, 2971, 3892, 4100, 4391, 4555
Bse3DI GCAATG 2 cut(s) 1711, 2223
Bse8I GATNNNNATC 5 cut(s) 1159, 3597, 3654, 4131, 4334
BseAI TCCGGA 1 cut(s) 2592
BseBI CCWGG 8 cut(s) 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 9 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024, 4502, 4580
BseJI GATNNNNATC 5 cut(s) 1159, 3597, 3654, 4131, 4334
BseLI CCNNNNNNNGG 7 cut(s) 45, 381, 2971, 3892, 4100, 4391, 4555
BseMI GCAATG 2 cut(s) 1711, 2223
BseMII CTCAG 6 cut(s) 352, 683, 1071, 2191, 2491, 3152
BseRI GAGGAG 4 cut(s) 311, 501, 2997, 4062
BseSI GKGCMC 2 cut(s) 3418, 3877
BseYI CCCAGC 2 cut(s) 2441, 3876
BsgI GTGCAG 2 cut(s) 1458, 3609
Bsh1236I CGCG 1 cut(s) 235
BshFI GGCC 6 cut(s) 165, 170, 1558, 1840, 3875, 4346
BshNI GGYRCC 1 cut(s) 3035
BshVI ATCGAT 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 2195, 3007
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 4 cut(s) 167, 248, 398, 2593
BslFI GGGAC 2 cut(s) 3448, 3507
BslI CCNNNNNNNGG 7 cut(s) 45, 381, 2971, 3892, 4100, 4391, 4555
BsmAI GTCTC 3 cut(s) 54, 215, 2651
BsmFI GGGAC 2 cut(s) 3448, 3507
BsmI GAATGC 1 cut(s) 2997
BsnI GGCC 6 cut(s) 165, 170, 1558, 1840, 3875, 4346
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 483
Bsp120I GGGCCC 1 cut(s) 3873
Bsp1286I GDGCHC 5 cut(s) 2195, 3007, 3227, 3418, 3877
Bsp13I TCCGGA 1 cut(s) 2592
Bsp1407I TGTACA 4 cut(s) 743, 1528, 3986, 4432
Bsp1720I GCTNAGC 1 cut(s) 2566
Bsp19I CCATGG 2 cut(s) 1008, 4024
BspANI GGCC 6 cut(s) 165, 170, 1558, 1840, 3875, 4346
BspCNI CTCAG 6 cut(s) 351, 682, 1070, 2190, 2490, 3153
BspDI ATCGAT 1 cut(s) 174
BspEI TCCGGA 1 cut(s) 2592
BspFNI CGCG 1 cut(s) 235
BspHI TCATGA 1 cut(s) 4338
BspLI GGNNCC 9 cut(s) 969, 1587, 3037, 3340, 3637, 3675, 3875, 4399, 4537
BspMAI CTGCAG 7 cut(s) 1309, 1390, 2960, 3493, 3541, 3663, 4146
BspMI ACCTGC 1 cut(s) 905
BspPI GGATC 6 cut(s) 1041, 1162, 1273, 2087, 3196, 4550
BspQI GCTCTTC 1 cut(s) 3147
BspT107I GGYRCC 1 cut(s) 3035
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 1711, 2223
BsrGI TGTACA 4 cut(s) 743, 1528, 3986, 4432
BssECI CCNNGG 9 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024, 4502, 4580
BssNAI GTATAC 1 cut(s) 2385
BssT1I CCWWGG 7 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024
Bst1107I GTATAC 1 cut(s) 2385
Bst2UI CCWGG 8 cut(s) 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
Bst6I CTCTTC 2 cut(s) 1549, 3147
BstAPI GCANNNNNTGC 3 cut(s) 1244, 3863, 4121
BstAUI TGTACA 4 cut(s) 743, 1528, 3986, 4432
BstBAI YACGTR 3 cut(s) 2348, 2390, 4097
BstC8I GCNNGC 5 cut(s) 1498, 2018, 2860, 2890, 4046
BstDEI CTNAG 9 cut(s) 338, 669, 851, 1057, 2028, 2177, 2477, 2566, 3161
BstDSI CCRYGG 2 cut(s) 1008, 4024
BstEII GGTNACC 2 cut(s) 790, 4510
BstFNI CGCG 1 cut(s) 235
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 2 cut(s) 120, 698
BstMAI GTCTC 3 cut(s) 54, 215, 2651
BstNI CCWGG 8 cut(s) 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
BstNSI RCATGY 5 cut(s) 907, 1209, 1694, 2762, 2892
BstPI GGTNACC 2 cut(s) 790, 4510
BstSCI CCNGG 9 cut(s) 165, 643, 786, 1150, 3799, 4384, 4501, 4538, 4580
BstSFI CTRYAG 8 cut(s) 1305, 1386, 2499, 2956, 3489, 3537, 3659, 4142
BstSLI GKGCMC 2 cut(s) 3418, 3877
BstUI CGCG 1 cut(s) 235
BstV2I GAAGAC 3 cut(s) 726, 966, 1757
BstX2I RGATCY 3 cut(s) 842, 1265, 3188
BstXI CCANNNNNNTGG 1 cut(s) 3374
BstYI RGATCY 3 cut(s) 842, 1265, 3188
BstZ17I GTATAC 1 cut(s) 2385
Bsu15I ATCGAT 1 cut(s) 174
BsuI GTATCC 1 cut(s) 3563
BsuRI GGCC 6 cut(s) 165, 170, 1558, 1840, 3875, 4346
BsuTUI ATCGAT 1 cut(s) 174
BtgI CCRYGG 2 cut(s) 1008, 4024
BtsI GCAGTG 2 cut(s) 947, 1302
BveI ACCTGC 1 cut(s) 905
Cac8I GCNNGC 5 cut(s) 1498, 2018, 2860, 2890, 4046
CaiI CAGNNNCTG 1 cut(s) 1958
CciI TCATGA 1 cut(s) 4338
CfoI GCGC 2 cut(s) 120, 698
Cfr13I GGNCC 6 cut(s) 164, 1556, 3246, 3609, 3873, 3874
ClaI ATCGAT 1 cut(s) 174
CseI GACGC 1 cut(s) 199
CsiI ACCWGGT 1 cut(s) 786
CspCI CAANNNNNGTGG 6 cut(s) 1347, 1382, 2611, 2646, 3700, 3735
DdeI CTNAG 9 cut(s) 338, 669, 851, 1057, 2028, 2177, 2477, 2566, 3161
DraI TTTAAA 3 cut(s) 1629, 2877, 3388
DraIII CACNNNGTG 4 cut(s) 1553, 2744, 3002, 4100
DriI GACNNNNNGTC 2 cut(s) 619, 1858
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 2 cut(s) 1549, 3147
Eam1105I GACNNNNNGTC 2 cut(s) 619, 1858
EarI CTCTTC 2 cut(s) 1549, 3147
EciI GGCGGA 2 cut(s) 66, 1734
Eco130I CCWWGG 7 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024
Eco147I AGGCCT 1 cut(s) 4346
Eco24I GRGCYC 2 cut(s) 3227, 3877
Eco31I GGTCTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 178
Eco47I GGWCC 2 cut(s) 3246, 3609
Eco57I CTGAAG 4 cut(s) 1379, 1920, 4425, 4583
Eco88I CYCGRG 1 cut(s) 483
Eco91I GGTNACC 2 cut(s) 790, 4510
EcoO65I GGTNACC 2 cut(s) 790, 4510
EcoRI GAATTC 3 cut(s) 333, 555, 3973
EcoRII CCWGG 8 cut(s) 643, 786, 1150, 3799, 4384, 4501, 4538, 4580
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 7 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024
EcoT22I ATGCAT 1 cut(s) 2754
EcoT38I GRGCYC 2 cut(s) 3227, 3877
ErhI CCWWGG 7 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024
FalI AAGNNNNNCTT 4 cut(s) 747, 779, 3123, 3155
FaqI GGGAC 2 cut(s) 3448, 3507
FauI CCCGC 1 cut(s) 116
FauNDI CATATG 1 cut(s) 3177
FbaI TGATCA 1 cut(s) 1927
FblI GTMKAC 1 cut(s) 2384
FriOI GRGCYC 2 cut(s) 3227, 3877
GlaI GCGC 2 cut(s) 119, 697
GsaI CCCAGC 2 cut(s) 2445, 3880
GsuI CTGGAG 4 cut(s) 890, 1134, 1425, 4209
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 6 cut(s) 165, 170, 1558, 1840, 3875, 4346
HapII CCGG 4 cut(s) 167, 248, 398, 2593
HgaI GACGC 1 cut(s) 199
HhaI GCGC 2 cut(s) 120, 698
Hin6I GCGC 2 cut(s) 118, 696
HinP1I GCGC 2 cut(s) 118, 696
HincII GTYRAC 3 cut(s) 1906, 2071, 3399
HindII GTYRAC 3 cut(s) 1906, 2071, 3399
HindIII AAGCTT 3 cut(s) 1880, 3091, 4565
HpaII CCGG 4 cut(s) 167, 248, 398, 2593
HphI GGTGA 5 cut(s) 993, 1539, 3105, 4331, 4430
Hpy166II GTNNAC 8 cut(s) 895, 1432, 1906, 2071, 2122, 2385, 2578, 3399
Hpy8I GTNNAC 8 cut(s) 895, 1432, 1906, 2071, 2122, 2385, 2578, 3399
Hpy99I CGWCG 5 cut(s) 164, 203, 206, 2767, 3397
HpyCH4IV ACGT 5 cut(s) 1133, 2347, 2389, 2588, 4096
HpyF3I CTNAG 9 cut(s) 338, 669, 851, 1057, 2028, 2177, 2477, 2566, 3161
HpySE526I ACGT 5 cut(s) 1133, 2347, 2389, 2588, 4096
HspAI GCGC 2 cut(s) 118, 696
Kpn2I TCCGGA 1 cut(s) 2592
KpnI GGTACC 1 cut(s) 3039
Ksp22I TGATCA 1 cut(s) 1927
LguI GCTCTTC 1 cut(s) 3147
MabI ACCWGGT 1 cut(s) 786
MaeII ACGT 5 cut(s) 1133, 2347, 2389, 2588, 4096
MaeIII GTNAC 9 cut(s) 187, 678, 790, 2087, 2107, 2126, 2472, 4183, 4510
MfeI CAATTG 1 cut(s) 2571
MflI RGATCY 3 cut(s) 842, 1265, 3188
MhlI GDGCHC 5 cut(s) 2195, 3007, 3227, 3418, 3877
MlyI GAGTC 9 cut(s) 238, 410, 629, 740, 2127, 2676, 2924, 2970, 3529
MmeI TCCRAC 8 cut(s) 63, 704, 1011, 2754, 3273, 3355, 3547, 4501
Mph1103I ATGCAT 1 cut(s) 2754
MroI TCCGGA 1 cut(s) 2592
MroXI GAANNNNTTC 6 cut(s) 759, 883, 2046, 3138, 3337, 3678
MslI CAYNNNNRTG 7 cut(s) 687, 902, 1982, 2631, 2757, 2887, 3176
MspA1I CMGCKG 4 cut(s) 1376, 2309, 2486, 4044
MspI CCGG 4 cut(s) 167, 248, 398, 2593
MspR9I CCNGG 9 cut(s) 167, 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
MunI CAATTG 1 cut(s) 2571
Mva1269I GAATGC 1 cut(s) 2997
MvaI CCWGG 8 cut(s) 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
MvnI CGCG 1 cut(s) 235
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 2 cut(s) 1008, 4024
NdeI CATATG 1 cut(s) 3177
NlaIV GGNNCC 9 cut(s) 969, 1587, 3037, 3340, 3637, 3675, 3875, 4399, 4537
NmeAIII GCCGAG 1 cut(s) 387
NmuCI GTSAC 2 cut(s) 187, 2087
NsiI ATGCAT 1 cut(s) 2754
NspI RCATGY 5 cut(s) 907, 1209, 1694, 2762, 2892
PaeI GCATGC 1 cut(s) 2892
PaeR7I CTCGAG 1 cut(s) 483
PagI TCATGA 1 cut(s) 4338
PaqCI CACCTGC 1 cut(s) 905
PceI AGGCCT 1 cut(s) 4346
PciI ACATGT 1 cut(s) 1205
PciSI GCTCTTC 1 cut(s) 3147
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 4093
PctI GAATGC 1 cut(s) 2997
PdmI GAANNNNTTC 6 cut(s) 759, 883, 2046, 3138, 3337, 3678
PflMI CCANNNNNTGG 3 cut(s) 2971, 3892, 4100
PfoI TCCNGGA 2 cut(s) 1150, 3799
PleI GAGTC 9 cut(s) 238, 409, 628, 739, 2126, 2675, 2924, 2969, 3528
PpsI GAGTC 9 cut(s) 238, 409, 628, 739, 2126, 2675, 2924, 2969, 3528
Ppu21I YACGTR 3 cut(s) 2348, 2390, 4097
PscI ACATGT 1 cut(s) 1205
PshBI ATTAAT 1 cut(s) 606
PsiI TTATAA 2 cut(s) 2412, 3776
Psp6I CCWGG 8 cut(s) 643, 786, 1150, 3799, 4384, 4501, 4538, 4580
PspEI GGTNACC 2 cut(s) 790, 4510
PspFI CCCAGC 2 cut(s) 2441, 3876
PspGI CCWGG 8 cut(s) 643, 786, 1150, 3799, 4384, 4501, 4538, 4580
PspN4I GGNNCC 9 cut(s) 969, 1587, 3037, 3340, 3637, 3675, 3875, 4399, 4537
PspOMI GGGCCC 1 cut(s) 3873
PspPI GGNCC 6 cut(s) 164, 1556, 3246, 3609, 3873, 3874
PspXI VCTCGAGB 1 cut(s) 483
PstI CTGCAG 7 cut(s) 1309, 1390, 2960, 3493, 3541, 3663, 4146
PstNI CAGNNNCTG 1 cut(s) 1958
PsuI RGATCY 3 cut(s) 842, 1265, 3188
PvuII CAGCTG 3 cut(s) 1376, 2309, 2486
RseI CAYNNNNRTG 7 cut(s) 687, 902, 1982, 2631, 2757, 2887, 3176
SapI GCTCTTC 1 cut(s) 3147
Sau96I GGNCC 6 cut(s) 164, 1556, 3246, 3609, 3873, 3874
ScaI AGTACT 3 cut(s) 2520, 2872, 2978
SchI GAGTC 9 cut(s) 238, 410, 629, 740, 2127, 2676, 2924, 2970, 3529
ScrFI CCNGG 9 cut(s) 167, 645, 788, 1152, 3801, 4386, 4503, 4540, 4582
SduI GDGCHC 5 cut(s) 2195, 3007, 3227, 3418, 3877
SexAI ACCWGGT 1 cut(s) 786
SfcI CTRYAG 8 cut(s) 1305, 1386, 2499, 2956, 3489, 3537, 3659, 4142
Sfr274I CTCGAG 1 cut(s) 483
SinI GGWCC 2 cut(s) 3246, 3609
SlaI CTCGAG 1 cut(s) 483
SmiI ATTTAAAT 1 cut(s) 3388
SmiMI CAYNNNNRTG 7 cut(s) 687, 902, 1982, 2631, 2757, 2887, 3176
SmlI CTYRAG 4 cut(s) 272, 483, 2274, 4076
SmoI CTYRAG 4 cut(s) 272, 483, 2274, 4076
SpeI ACTAGT 1 cut(s) 1889
SphI GCATGC 1 cut(s) 2892
SseBI AGGCCT 1 cut(s) 4346
SspI AATATT 3 cut(s) 2640, 2693, 3757
StuI AGGCCT 1 cut(s) 4346
StyD4I CCNGG 9 cut(s) 165, 643, 786, 1150, 3799, 4384, 4501, 4538, 4580
StyI CCWWGG 7 cut(s) 135, 435, 1008, 3253, 3639, 3925, 4024
SwaI ATTTAAAT 1 cut(s) 3388
TaiI ACGT 5 cut(s) 1136, 2350, 2392, 2591, 4099
TatI WGTACW 9 cut(s) 743, 1528, 1684, 2480, 2518, 2870, 2976, 3986, 4432
TauI GCSGC 2 cut(s) 82, 361
TseFI GTSAC 2 cut(s) 187, 2087
Tsp45I GTSAC 2 cut(s) 187, 2087
TspGWI ACGGA 2 cut(s) 960, 2711
Van91I CCANNNNNTGG 3 cut(s) 2971, 3892, 4100
VpaK11BI GGWCC 2 cut(s) 3246, 3609
VspI ATTAAT 1 cut(s) 606
XbaI TCTAGA 1 cut(s) 3142
XceI RCATGY 5 cut(s) 907, 1209, 1694, 2762, 2892
XcmI CCANNNNNNNNNTGG 1 cut(s) 2558
XhoI CTCGAG 1 cut(s) 483
XmaJI CCTAGG 1 cut(s) 3925
XmiI GTMKAC 1 cut(s) 2384
XmnI GAANNNNTTC 6 cut(s) 759, 883, 2046, 3138, 3337, 3678
ZrmI AGTACT 3 cut(s) 2520, 2872, 2978
Zsp2I ATGCAT 1 cut(s) 2754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.