MD16G1052600.v1.1

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006265
Physical Location & Seq
Forward (+)
3754626 .. 3764152
9527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1052600.v1.1.491

Sequence Viewer

Length: 5145 bp
ATGCTATTACTCACAAATCCTCTCTCTTTCCGATGTTGCAAGAGGTCTCTGTCTCTCCCGCGCACAATGCCTCCCTCACCGCCACCGCCTTTCCCGTTCTTCAACCCGAAGTCCCGCCATTTTCGTCTCCCTCCAAACCCTAATTCCAAGGCCGTTGGAGGAGTAAGATTCGACGGCCCGGCGATCGAAGTTGGCCAAGTGTCGGAAGCCGAGGACGGCGACTTGGTGATCGAGACCGGCGTCACGCGGATGCTGCCTCCGGCTTTGACTCTTCAGCAAGGACTTGAAAAGATCAGAGAGGCTCTTGAGGAGTTGAAGCTCAATCCTCCTTCCACATGTACCGGAATTCATAGGTTTCAGGTGGCCGTCCCCCCGAGTGCCAAAGCCTTGTACTGGTTTTGCAGTCAACCGGAGACGTCTGCGGTTTATCCTCTGTTTTTCATTGGCAAGGATGCCGAAACCCCGAGTTATAAGTCGCTGTATGCAAATGAAACTCGAGGAGTATTCGGAATTGGTGCCGCGGTTCACTTTGCACCTTCATCATCTTCTTCTTCGGTCAAAAGATATCTTTCAAATGAATCAACCTCTGTAATGTCTTATGGTTTGGTGGATATTAATTACGACCACGAGTCATCTTTCATAAAACATGAAGCAGGTTCTTACTACTGTTTTGTTCCTCAGATTGAGTTACATGAGTACGAGGGCGCTTCTGTTTTAGCAGCAACAATTGCTTGGAGTGACTCTTCCGTTTGTACATTTGAGGAAGCCATTCATTCTTTTGAGTTATGTTTCGACCAGGCTAGTTGTCACTGTTGGCCCGCGCCAAAGAGCTCCCACTCCATGAATATAAGACGTACTCTTGGAAAGCTCAAGCTTCATGAGGATGGAATTGTTCCTATGGTATACATGAATTCTCTGTCATCAAGCGGAAAATATGTTGTGGCTAACATCACGACGCTGAAAGAGACTCCGTTTTCCTGCCAGTTTTGTATCAAGCTTTCACCTACCATTGCAGTTTCTAGCAACATGTTGGATCATGCCAATAAAATGTGCTACTCAGTTGAAGATTGTGCTAACATCAATACTATTTGGGCATCACTTATAATTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCTCTTGCTGTTGCTGCATCCACTCATCCTCTTATTACATGCATCGTATGTTACGATGAGCGCTCACTTGCATTTCATGCCGTTGGTTATGCAAGAGGATCTCAGAAGCCAGCAGTAGTCATAACTTCATCTGGAACTGCAGTTTCAAACCTTCTTCCTGCTGTTGTTGAAGCTAGTCAAGATTTTGTACCACTCCTATTGCTCACAGCTGACCGTCCTGCAGAATTGCATGATGCTGGAGCAAACCAAGCTATCAATCAGGTAAACCATTTTGGTTCTTTTGTGAGGTTTTTTTTCAGCCTTCCAGCAGCCACCGATCATATTTCAGCAAGGATGGTACTTACAACACTTGACTCTGCTGTACATTGGGCAACCTCATCACCGTGTGGCCCCGTTCATATTAACTGTCCTTTCAGGGAACCACTGGAAAATAGCCCTAGCAAATGGATGACGAGTTGTTTGAAAGGATTAGACTTTTGGATGTCTAGCACCGAGCCGTTTACTAAGTATATTAAACTACAGAGTGCTCACACGTATGATGATGGTTGTGGGCAAATGTCAGAGATTCTAAATCTAATTAGAGGGACTAATAAAGGGATTCTACTAATTGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTTTTGGTTAAACACCTTCAATGGCCTGTTGTAGCTGACATCCTGTCAGGATTACGATTGAGAAAGCTGCTGACTTCTTTTCCTGAAATTGGAGATGATTTGTTGTTTGTTGATCATCTTGACCATGCTCTACTTTCAGATTCCATGAGTAGTTGGATAAATTTTGATCTGATTATTCAGATTGGGAGTAGGATTACAAGCAAGCGTGTTGCTAAAATGCTAGAGGACTGCTTTCCCTGCTCATATATCTTGGTTGACAAGCATCCATTTCGTCATGATCCCTCTCATATTGTAACCCATAGAATCCAAAGTAGCATTGTTGAATTTTCTGATTGTTTATGCAAAGCTGGGCTTCCCTGTATGAGCAAAGAATGGAGCACTTATCTTCAAACGTTAAATGTGATGGTTTCGAGGGAGTTGTCGTTCCAAATTTATGCCAGAGACTTCTTGACTGAACCTCAAGTTGCAAGCGTAATATCCAAAGCTCTTTCAGCTGAGTCTGCTCTTTTCATTGGGAACAGTATGGCGATACGTGATGCAGATATGTACGGATGTGGTTGGTCAGGTTGTAGTCATAATATTGCATCCATGATTTCAAAATTAGAACTACCCTGTCATATGATTCGGGTGGCTGGGAATAGGGGTGCCAGTGGTATTGATGGGTTACTCAGCACAGCTGTTGGCTTTGCTGTTGGATGCAATAAACGAGTGCTTTGCGTGATTGGGGATGTTTCTTTCCTCCACGATACAAATGGCTTGGCAATTGTGAACCAGAGGACATTGCGGAAACCAATGACGATACTAGTGATCAATAATCATGGTGGTGCAATATTCAGTCTCCTTCCTATAGCGGATAGAGTTGAGCCAAGCATACTAAATCAATACTTCTACACCTCTCACAATGTTTCAATCCACAACCTGTGTGCGGCCCATGGTGTGATGCATCTACATGTGAAGACAAAGGTGGAGCTTGAAGATGCATTATTGACATCTCAAGATAATGAGGTAGATTGTGTCATTGAAGTTGAGAGCTGCATAGAAGCCAATGCCACCTTTCACAGTACTTTAAGAAAGTTTGCGTGCCAAGCAGCAGATCATGCGTTGAGCCTCTCCTCAAGGATTTCTGTTCAAGACTCACCAGCAGATGGCACTTTACTTTATAGAGTCCATAGAATGGAGTATTCAGTATTTAGTATACCACTCTGTGCTCCTCCTACTATGGTTTCTGTTGATGAAAATGAAACCAGCTTCTACAGAGAAGGGTTTATTTTAACTTTGTATCTTGAGGATGGAAGCATTGGGTTTGGCGAGGTTTCACCTCTCGATATCCACAGAGAAAACCTGCTCGATGTAGAAGAGCAACTGAGGTTACTAGTTCATATGATGGAAGGAGCTAAGATTAGTTGCTTCCTTCCTCTGCTAAAGGGCTCTTTTTCTTCTTGGATATGGACCAACCTGGGAATACTGCCATGCACGCTCTTGCCTAGTGTTAGATGTGGTTTGGAAATGGCAATTCTCAATGCATTAGCGACAAGGCAAGGTTCCAACTTATTAGGCCTACTCCATCCCTTGAAAGCTGAAGGAGGTATATCTGAAAGGCCGATGACAGTTCAGATTTGTGCCCTAGTTGATTCCAATGGAACGCCCACACAAGTAGCTGATGTTGTAGCAGCACTTGTTGAAGAAGGATTTACTGCAGTAAAGCTGAAGGTAGCACGTCAGGGGAGTCCCTTGCACGATGCTGCAGTTATACAGGCAGTAAGAAAGAAAATTGGGTACCAGATTGAAGTTCGTGCAGATGCCAATCGGAACTGGACATATAAAGAAGCTATCCAATTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATTGAGGAACCTGTTCAAAGCGAAGGTGATATTGTCAAGTTCTGTGAAGAAAGCGGGTTACCCGTGGCACTGGATGAAACTATTGACAGTATTAGAGAACATCCACTTGACAAGCTAATGAAGTACACCCATCCAGGAATAGTAGCTATTGTTATTAAACCTAGTGTTGTTGGTGGCTTTGAAAATGCGGCAATAATTGCCCAATGGGCTCAGCAGCACCAGAAAATGGCTGTTGTTAGTGCTGCATTTGAAAGTGGCCTAGGTTTGTCAGCATATATTCAGTTTTGCTGTTATCTCAACCTGAAAAATTCAGAAATCTGTGAAATGATGAATTATGAATTGGCCCCATCAATAGCCCATGGTTTAGGAACATACCGCTGGCTAAAAGAAGATGTAACAACCACTCCTCTCAAGATAGGTTGTAATCCAGTCAGTGGCTTCATAGAAGCATCTGTTGCTGATGCTGATCAAGTTCTTCAAAAATTTCAAATAAATGGCAATGTAGTTCATAGAAATTTTACTGGAGAGCAAGTCCGCGTATTCCAGCTTACTGTAGATTCAAGGGCTTTCTCTTATTCCATCATAGTCCACGAGATTGGAGAAAGATACAATGAAAATGTTTTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATGATGAAGGCGATTTCAGGATGTGCAAGATGCATTTCGATTGACCTTCCTGGTCATGGTGGAACAAAGATACAGAACCATGGTGATAACGATGCTGTACAGGATTCAGGTTTGTCAATTGAGGTTGTTGCCGATCTTTTATGTGAAGTGATTAAACATATTACGCCTGGAAAGGTTACTATTGTGGGTTACTCTATGGGAGCAAGGATTGCACTGTACATGGCACTGAGGTGTACCGACAAAGTTAATGGAGCTGTTGTAATATCTGGAAGCCCTGGGTTAAAAGATGAAGTGGCAAGAAAAATTCGTAGAGCTAAAGATGACTCTAGAGCTCGCATCCTTATTGATCACGGCTTAGAGCTCTTCATAGACACCTGGTATTCCGGAGAGCTGTGGAACAGCTTAAGAGTCCATCCCCGATTTCGTCAGATAGTTTCCAGCCGCCTGCATCATGAAGATGTGCATAGTCTTGCGAAAGTGCTTTCCGGTTTAAGTATTGGGAGGCAGCCGCCATTGTGGGAAGACTTGAAGCACTGCAAAACACCCCTTTTGCTAATTGTTGGAGAGGAGGATAAAAAGTTCAAGGCGATAGCTGAGGATATGTCCTGTGAAATTGCTGGTGGCACGGCGAGTGGAGACAGCCCTCGTCATGACATCTATGAGATAGTTGAGATTCCAGATTGCGGACATGCTGCTCATCTGGAGAACCCTCTCCCTGTAATCAGCACGTTGAGACGATTTTTGGGGAGAGTGAACTCGAGTTTGATTCAAAACCAGAAGGCAATTTAG

Protein Analysis

1715

Amino Acids

188.38

Weight (kDa)

5.93

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 365 - 477 5.5e-21 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 543 - 776 1.3e-20 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 820 - 947 4.2e-08 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 1157 - 1343 2.3e-26 Enolase C-terminal domain-like
Abhydrolase_1 PF00561 1435 - 1689 2e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 1437 - 1692 6.9e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 1437 - 1644 1e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 471, 1103
AatII GACGTC 1 cut(s) 419
Acc36I ACCTGC 2 cut(s) 644, 3165
Acc65I GGTACC 1 cut(s) 3591
AccB1I GGYRCC 3 cut(s) 515, 2459, 3591
AccB7I CCANNNNNTGG 4 cut(s) 2960, 2989, 3913, 4121
AccI GTMKAC 2 cut(s) 903, 3010
AccII CGCG 5 cut(s) 61, 247, 521, 821, 4224
AccIII TCCGGA 1 cut(s) 4739
AclI AACGTT 1 cut(s) 2207
AclWI GGATC 4 cut(s) 1041, 1162, 1273, 2087
AcoI YGGCCR 2 cut(s) 193, 363
AcsI RAATTY 9 cut(s) 345, 910, 1975, 2138, 2244, 3994, 4169, 4201, 4659
AcuI CTGAAG 3 cut(s) 257, 3414, 3542
AcyI GRCGYC 2 cut(s) 240, 416
AdeI CACNNNGTG 2 cut(s) 1553, 3020
AfeI AGCGCT 1 cut(s) 1229
AflII CTTAAG 1 cut(s) 4759
AflIII ACRYGT 4 cut(s) 335, 1026, 1698, 2764
AhdI GACNNNNNGTC 2 cut(s) 628, 1858
AhlI ACTAGT 2 cut(s) 2617, 3187
AjiI CACGTC 1 cut(s) 3533
AjnI CCWGG 8 cut(s) 795, 1150, 3270, 3820, 4405, 4522, 4630, 4730
AjuI GAANNNNNNNTTGG 4 cut(s) 127, 159, 4010, 4042
AleI CACNNNNGTG 1 cut(s) 4585
AloI GAACNNNNNNTCC 4 cut(s) 2222, 2254, 4919, 4951
Alw21I GWGCWC 6 cut(s) 833, 1696, 2195, 3025, 4690, 4719
AlwI GGATC 4 cut(s) 1041, 1162, 1273, 2087
Ama87I CYCGRG 4 cut(s) 373, 463, 495, 5113
Aor13HI TCCGGA 1 cut(s) 4739
Aor51HI AGCGCT 1 cut(s) 1229
ApoI RAATTY 9 cut(s) 345, 910, 1975, 2138, 2244, 3994, 4169, 4201, 4659
ArsI GACNNNNNNTTYG 2 cut(s) 1609, 1641
AseI ATTAAT 1 cut(s) 615
Asp700I GAANNNNTTC 2 cut(s) 768, 3699
Asp718I GGTACC 1 cut(s) 3591
AspA2I CCTAGG 1 cut(s) 3946
AspLEI GCGC 4 cut(s) 63, 707, 823, 1230
AspS9I GGNCC 6 cut(s) 176, 816, 1556, 2743, 3264, 4030
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 9 cut(s) 69, 238, 993, 1539, 2943, 3123, 3725, 4352, 4451
AvaI CYCGRG 4 cut(s) 373, 463, 495, 5113
AvaII GGWCC 1 cut(s) 3264
AvrII CCTAGG 1 cut(s) 3946
BaeGI GKGCMC 1 cut(s) 3439
BalI TGGCCA 1 cut(s) 195
BanI GGYRCC 3 cut(s) 515, 2459, 3591
BanII GRGCYC 5 cut(s) 833, 3245, 3898, 4690, 4719
BauI CACGAG 2 cut(s) 626, 4277
BbsI GAAGAC 2 cut(s) 2777, 4884
Bbv12I GWGCWC 6 cut(s) 833, 1696, 2195, 3025, 4690, 4719
BbvCI CCTCAGC 1 cut(s) 4950
BceAI ACGGC 8 cut(s) 137, 190, 232, 350, 1232, 1648, 4723, 4998
BcgI CGANNNNNNTGC 4 cut(s) 2066, 2100, 2511, 2545
BciT130I CCWGG 8 cut(s) 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
BclI TGATCA 4 cut(s) 1927, 2622, 4153, 4702
BcnI CCSGG 1 cut(s) 179
BcuI ACTAGT 2 cut(s) 2617, 3187
BfmI CTRYAG 9 cut(s) 1305, 1386, 1685, 2661, 3067, 3510, 3558, 3680, 4239
BfoI RGCGCY 2 cut(s) 708, 1231
BfrI CTTAAG 1 cut(s) 4759
BfuAI ACCTGC 2 cut(s) 644, 3165
BglI GCCNNNNNGGC 1 cut(s) 3893
BlnI CCTAGG 1 cut(s) 3946
BlpI GCTNAGC 1 cut(s) 3897
BmcAI AGTACT 1 cut(s) 2878
Bme1390I CCNGG 9 cut(s) 179, 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
Bme18I GGWCC 1 cut(s) 3264
BmeRI GACNNNNNGTC 2 cut(s) 628, 1858
BmeT110I CYCGRG 4 cut(s) 373, 463, 495, 5113
BmgBI CACGTC 1 cut(s) 3533
BmgT120I GGNCC 6 cut(s) 176, 816, 1556, 2743, 3264, 4030
BmiI GGNNCC 9 cut(s) 517, 1558, 1587, 2461, 3358, 3593, 3658, 3696, 4032
BmrFI CCNGG 9 cut(s) 179, 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
BoxI GACNNNNGTC 1 cut(s) 239
BpiI GAAGAC 2 cut(s) 2777, 4884
BpmI CTGGAG 4 cut(s) 1134, 1425, 4230, 5078
Bpu10I CCTNAGC 1 cut(s) 4950
Bpu1102I GCTNAGC 1 cut(s) 3897
BpuEI CTTGAG 7 cut(s) 326, 854, 2259, 2793, 2914, 3119, 4082
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 2 cut(s) 1701, 2348
BsaBI GATNNNNATC 4 cut(s) 1159, 3618, 3675, 4152
BsaHI GRCGYC 2 cut(s) 240, 416
BsaI GGTCTC 2 cut(s) 51, 227
BsaWI WCCGGW 4 cut(s) 341, 409, 4739, 4841
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 9 cut(s) 398, 982, 1596, 2463, 3632, 3762, 4115, 4213, 4342
Bse3DI GCAATG 3 cut(s) 1008, 2594, 4192
Bse8I GATNNNNATC 4 cut(s) 1159, 3618, 3675, 4152
BseAI TCCGGA 1 cut(s) 4739
BseBI CCWGG 8 cut(s) 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
BseJI GATNNNNATC 4 cut(s) 1159, 3618, 3675, 4152
BseMI GCAATG 3 cut(s) 1008, 2594, 4192
BseMII CTCAG 9 cut(s) 692, 1071, 1283, 2301, 2497, 3170, 3911, 4574, 4941
BseNI ACTGG 9 cut(s) 398, 982, 1596, 2463, 3632, 3762, 4115, 4213, 4342
BseRI GAGGAG 7 cut(s) 174, 323, 513, 2917, 3015, 4083, 4937
BseSI GKGCMC 1 cut(s) 3439
BseYI CCCAGC 2 cut(s) 2162, 2447
BsgI GTGCAG 1 cut(s) 3630
Bsh1236I CGCG 5 cut(s) 61, 247, 521, 821, 4224
Bsh1285I CGRYCG 1 cut(s) 186
BshNI GGYRCC 3 cut(s) 515, 2459, 3591
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 6 cut(s) 833, 1696, 2195, 3025, 4690, 4719
BsiHKCI CYCGRG 4 cut(s) 373, 463, 495, 5113
BsiSI CCGG 7 cut(s) 179, 237, 260, 342, 410, 4740, 4842
BslFI GGGAC 4 cut(s) 97, 353, 1765, 3528
BsmBI CGTCTC 3 cut(s) 131, 407, 5083
BsmFI GGGAC 4 cut(s) 97, 353, 1765, 3528
Bso31I GGTCTC 2 cut(s) 51, 227
BsoBI CYCGRG 4 cut(s) 373, 463, 495, 5113
Bsp1286I GDGCHC 9 cut(s) 833, 1696, 2195, 3025, 3245, 3439, 3898, 4690, 4719
Bsp13I TCCGGA 1 cut(s) 4739
Bsp1407I TGTACA 4 cut(s) 752, 1528, 4453, 4572
Bsp1720I GCTNAGC 1 cut(s) 3897
Bsp19I CCATGG 3 cut(s) 2746, 4045, 4435
BspCNI CTCAG 9 cut(s) 691, 1070, 1282, 2302, 2496, 3171, 3910, 4575, 4942
BspEI TCCGGA 1 cut(s) 4739
BspFNI CGCG 5 cut(s) 61, 247, 521, 821, 4224
BspHI TCATGA 4 cut(s) 877, 2089, 4807, 5005
BspLI GGNNCC 9 cut(s) 517, 1558, 1587, 2461, 3358, 3593, 3658, 3696, 4032
BspMAI CTGCAG 5 cut(s) 1309, 1390, 3514, 3562, 3684
BspMI ACCTGC 2 cut(s) 644, 3165
BspPI GGATC 4 cut(s) 1041, 1162, 1273, 2087
BspQI GCTCTTC 2 cut(s) 3165, 4724
BspT107I GGYRCC 3 cut(s) 515, 2459, 3591
BspTI CTTAAG 1 cut(s) 4759
BspTNI GGTCTC 2 cut(s) 51, 227
BsrDI GCAATG 3 cut(s) 1008, 2594, 4192
BsrFI RCCGGY 1 cut(s) 236
BsrGI TGTACA 4 cut(s) 752, 1528, 4453, 4572
BsrI ACTGG 9 cut(s) 398, 982, 1596, 2463, 3632, 3762, 4115, 4213, 4342
BssAI RCCGGY 1 cut(s) 236
BssNAI GTATAC 2 cut(s) 904, 3011
BssNI GRCGYC 2 cut(s) 240, 416
BssSI CACGAG 2 cut(s) 626, 4277
BssT1I CCWWGG 6 cut(s) 147, 2746, 3660, 3946, 4045, 4435
Bst1107I GTATAC 2 cut(s) 904, 3011
Bst2BI CACGAG 2 cut(s) 626, 4277
Bst2UI CCWGG 8 cut(s) 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
Bst6I CTCTTC 4 cut(s) 276, 748, 3165, 4724
BstACI GRCGYC 2 cut(s) 240, 416
BstAFI CTTAAG 1 cut(s) 4759
BstAPI GCANNNNNTGC 6 cut(s) 728, 1244, 2912, 3884, 4142, 4565
BstAUI TGTACA 4 cut(s) 752, 1528, 4453, 4572
BstBAI YACGTR 2 cut(s) 1701, 2348
BstC8I GCNNGC 9 cut(s) 819, 1278, 2018, 2284, 2896, 3290, 4067, 4690, 4802
BstDSI CCRYGG 5 cut(s) 519, 2746, 3750, 4045, 4435
BstEII GGTNACC 1 cut(s) 3744
BstFNI CGCG 5 cut(s) 61, 247, 521, 821, 4224
BstH2I RGCGCY 2 cut(s) 708, 1231
BstHHI GCGC 4 cut(s) 63, 707, 823, 1230
BstMCI CGRYCG 1 cut(s) 186
BstNI CCWGG 8 cut(s) 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
BstNSI RCATGY 5 cut(s) 339, 1030, 1209, 2768, 5048
BstPAI GACNNNNGTC 1 cut(s) 239
BstPI GGTNACC 1 cut(s) 3744
BstSCI CCNGG 9 cut(s) 177, 795, 1150, 3270, 3820, 4405, 4522, 4630, 4730
BstSFI CTRYAG 9 cut(s) 1305, 1386, 1685, 2661, 3067, 3510, 3558, 3680, 4239
BstSLI GKGCMC 1 cut(s) 3439
BstUI CGCG 5 cut(s) 61, 247, 521, 821, 4224
BstV2I GAAGAC 2 cut(s) 2777, 4884
BstX2I RGATCY 1 cut(s) 1265
BstXI CCANNNNNNTGG 1 cut(s) 4283
BstYI RGATCY 1 cut(s) 1265
BstZ17I GTATAC 2 cut(s) 904, 3011
BtgI CCRYGG 5 cut(s) 519, 2746, 3750, 4045, 4435
BtrI CACGTC 1 cut(s) 3533
BtsI GCAGTG 1 cut(s) 4888
BtsIMutI CAGTG 8 cut(s) 808, 1589, 2470, 3755, 4126, 4568, 4580, 4888
BveI ACCTGC 2 cut(s) 644, 3165
Cac8I GCNNGC 9 cut(s) 819, 1278, 2018, 2284, 2896, 3290, 4067, 4690, 4802
CciI TCATGA 4 cut(s) 877, 2089, 4807, 5005
CfoI GCGC 4 cut(s) 63, 707, 823, 1230
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 6 cut(s) 176, 816, 1556, 2743, 3264, 4030
Cfr42I CCGCGG 1 cut(s) 522
CseI GACGC 2 cut(s) 229, 964
CsiI ACCWGGT 1 cut(s) 4730
CspCI CAANNNNNGTGG 2 cut(s) 1347, 1382
DraIII CACNNNGTG 2 cut(s) 1553, 3020
DriI GACNNNNNGTC 2 cut(s) 628, 1858
EaeI YGGCCR 2 cut(s) 193, 363
Eam1104I CTCTTC 4 cut(s) 276, 748, 3165, 4724
Eam1105I GACNNNNNGTC 2 cut(s) 628, 1858
EarI CTCTTC 4 cut(s) 276, 748, 3165, 4724
Ecl136II GAGCTC 3 cut(s) 831, 4688, 4717
Eco130I CCWWGG 6 cut(s) 147, 2746, 3660, 3946, 4045, 4435
Eco147I AGGCCT 1 cut(s) 3372
Eco24I GRGCYC 5 cut(s) 833, 3245, 3898, 4690, 4719
Eco31I GGTCTC 2 cut(s) 51, 227
Eco32I GATATC 2 cut(s) 566, 3142
Eco47I GGWCC 1 cut(s) 3264
Eco47III AGCGCT 1 cut(s) 1229
Eco53kI GAGCTC 3 cut(s) 831, 4688, 4717
Eco57I CTGAAG 3 cut(s) 257, 3414, 3542
Eco88I CYCGRG 4 cut(s) 373, 463, 495, 5113
Eco91I GGTNACC 1 cut(s) 3744
EcoICRI GAGCTC 3 cut(s) 831, 4688, 4717
EcoO65I GGTNACC 1 cut(s) 3744
EcoRI GAATTC 2 cut(s) 345, 910
EcoRII CCWGG 8 cut(s) 795, 1150, 3270, 3820, 4405, 4522, 4630, 4730
EcoRV GATATC 2 cut(s) 566, 3142
EcoT14I CCWWGG 6 cut(s) 147, 2746, 3660, 3946, 4045, 4435
EcoT22I ATGCAT 5 cut(s) 1211, 2760, 2797, 3340, 4391
EcoT38I GRGCYC 5 cut(s) 833, 3245, 3898, 4690, 4719
ErhI CCWWGG 6 cut(s) 147, 2746, 3660, 3946, 4045, 4435
Esp3I CGTCTC 3 cut(s) 131, 407, 5083
FalI AAGNNNNNCTT 2 cut(s) 2151, 2183
FaqI GGGAC 4 cut(s) 97, 353, 1765, 3528
FauI CCCGC 4 cut(s) 66, 122, 826, 3734
FauNDI CATATG 2 cut(s) 2433, 3195
FbaI TGATCA 4 cut(s) 1927, 2622, 4153, 4702
FblI GTMKAC 2 cut(s) 903, 3010
FriOI GRGCYC 5 cut(s) 833, 3245, 3898, 4690, 4719
GlaI GCGC 4 cut(s) 62, 706, 822, 1229
GsaI CCCAGC 2 cut(s) 2166, 2451
GsuI CTGGAG 4 cut(s) 1134, 1425, 4230, 5078
HaeII RGCGCY 2 cut(s) 708, 1231
HapII CCGG 7 cut(s) 179, 237, 260, 342, 410, 4740, 4842
HgaI GACGC 2 cut(s) 229, 964
HhaI GCGC 4 cut(s) 63, 707, 823, 1230
Hin1I GRCGYC 2 cut(s) 240, 416
Hin6I GCGC 4 cut(s) 61, 705, 821, 1228
HinP1I GCGC 4 cut(s) 61, 705, 821, 1228
HincII GTYRAC 2 cut(s) 407, 2071
HindII GTYRAC 2 cut(s) 407, 2071
HindIII AAGCTT 2 cut(s) 872, 995
HpaII CCGG 7 cut(s) 179, 237, 260, 342, 410, 4740, 4842
HphI GGTGA 9 cut(s) 69, 238, 993, 1539, 2943, 3123, 3725, 4352, 4451
Hpy99I CGWCG 2 cut(s) 176, 958
HpyCH4IV ACGT 8 cut(s) 416, 853, 1133, 1700, 2207, 2347, 3532, 5084
HpySE526I ACGT 8 cut(s) 416, 853, 1133, 1700, 2207, 2347, 3532, 5084
Hsp92I GRCGYC 2 cut(s) 240, 416
HspAI GCGC 4 cut(s) 61, 705, 821, 1228
Kpn2I TCCGGA 1 cut(s) 4739
KpnI GGTACC 1 cut(s) 3595
Ksp22I TGATCA 4 cut(s) 1927, 2622, 4153, 4702
KspI CCGCGG 1 cut(s) 522
LguI GCTCTTC 2 cut(s) 3165, 4724
LmnI GCTCC 9 cut(s) 836, 1153, 1406, 2190, 2782, 3028, 3206, 4556, 4607
MabI ACCWGGT 1 cut(s) 4730
MaeII ACGT 8 cut(s) 416, 853, 1133, 1700, 2207, 2347, 3532, 5084
MfeI CAATTG 3 cut(s) 726, 2577, 4473
MflI RGATCY 1 cut(s) 1265
MhlI GDGCHC 9 cut(s) 833, 1696, 2195, 3025, 3245, 3439, 3898, 4690, 4719
MlsI TGGCCA 1 cut(s) 195
MluNI TGGCCA 1 cut(s) 195
MmeI TCCRAC 7 cut(s) 136, 183, 1011, 1949, 2488, 3384, 4897
Mox20I TGGCCA 1 cut(s) 195
Mph1103I ATGCAT 5 cut(s) 1211, 2760, 2797, 3340, 4391
MroI TCCGGA 1 cut(s) 4739
MroXI GAANNNNTTC 2 cut(s) 768, 3699
MscI TGGCCA 1 cut(s) 195
MslI CAYNNNNRTG 8 cut(s) 248, 882, 1549, 1701, 2637, 2763, 4585, 4812
Msp20I TGGCCA 1 cut(s) 195
MspA1I CMGCKG 5 cut(s) 521, 1376, 2309, 2492, 4065
MspCI CTTAAG 1 cut(s) 4759
MspI CCGG 7 cut(s) 179, 237, 260, 342, 410, 4740, 4842
MspR9I CCNGG 9 cut(s) 179, 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
MunI CAATTG 3 cut(s) 726, 2577, 4473
MvaI CCWGG 8 cut(s) 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
MvnI CGCG 5 cut(s) 61, 247, 521, 821, 4224
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 3 cut(s) 2746, 4045, 4435
NdeI CATATG 2 cut(s) 2433, 3195
NlaIV GGNNCC 9 cut(s) 517, 1558, 1587, 2461, 3358, 3593, 3658, 3696, 4032
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 3 cut(s) 241, 737, 806
NsiI ATGCAT 5 cut(s) 1211, 2760, 2797, 3340, 4391
NspI RCATGY 5 cut(s) 339, 1030, 1209, 2768, 5048
OliI CACNNNNGTG 1 cut(s) 4585
PaeR7I CTCGAG 2 cut(s) 495, 5113
PagI TCATGA 4 cut(s) 877, 2089, 4807, 5005
PasI CCCWGGG 1 cut(s) 4631
PceI AGGCCT 1 cut(s) 3372
PciI ACATGT 3 cut(s) 335, 1026, 2764
PciSI GCTCTTC 2 cut(s) 3165, 4724
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 1218
PdmI GAANNNNTTC 2 cut(s) 768, 3699
PflMI CCANNNNNTGG 4 cut(s) 2960, 2989, 3913, 4121
PfoI TCCNGGA 2 cut(s) 1150, 3820
Ple19I CGATCG 1 cut(s) 186
Ppu21I YACGTR 2 cut(s) 1701, 2348
PscI ACATGT 3 cut(s) 335, 1026, 2764
PshAI GACNNNNGTC 1 cut(s) 239
PshBI ATTAAT 1 cut(s) 615
PsiI TTATAA 2 cut(s) 471, 1103
Psp124BI GAGCTC 3 cut(s) 833, 4690, 4719
Psp1406I AACGTT 1 cut(s) 2207
Psp6I CCWGG 8 cut(s) 795, 1150, 3270, 3820, 4405, 4522, 4630, 4730
PspEI GGTNACC 1 cut(s) 3744
PspFI CCCAGC 2 cut(s) 2162, 2447
PspGI CCWGG 8 cut(s) 795, 1150, 3270, 3820, 4405, 4522, 4630, 4730
PspN4I GGNNCC 9 cut(s) 517, 1558, 1587, 2461, 3358, 3593, 3658, 3696, 4032
PspPI GGNCC 6 cut(s) 176, 816, 1556, 2743, 3264, 4030
PspXI VCTCGAGB 2 cut(s) 495, 5113
PstI CTGCAG 5 cut(s) 1309, 1390, 3514, 3562, 3684
PsuI RGATCY 1 cut(s) 1265
PvuI CGATCG 1 cut(s) 186
PvuII CAGCTG 3 cut(s) 1376, 2309, 2492
RseI CAYNNNNRTG 8 cut(s) 248, 882, 1549, 1701, 2637, 2763, 4585, 4812
SacI GAGCTC 3 cut(s) 833, 4690, 4719
SacII CCGCGG 1 cut(s) 522
SapI GCTCTTC 2 cut(s) 3165, 4724
Sau96I GGNCC 6 cut(s) 176, 816, 1556, 2743, 3264, 4030
ScaI AGTACT 1 cut(s) 2878
ScrFI CCNGG 9 cut(s) 179, 797, 1152, 3272, 3822, 4407, 4524, 4632, 4732
SduI GDGCHC 9 cut(s) 833, 1696, 2195, 3025, 3245, 3439, 3898, 4690, 4719
SexAI ACCWGGT 1 cut(s) 4730
SfcI CTRYAG 9 cut(s) 1305, 1386, 1685, 2661, 3067, 3510, 3558, 3680, 4239
Sfr274I CTCGAG 2 cut(s) 495, 5113
Sfr303I CCGCGG 1 cut(s) 522
SgrBI CCGCGG 1 cut(s) 522
SinI GGWCC 1 cut(s) 3264
SlaI CTCGAG 2 cut(s) 495, 5113
SmiMI CAYNNNNRTG 8 cut(s) 248, 882, 1549, 1701, 2637, 2763, 4585, 4812
SpeI ACTAGT 2 cut(s) 2617, 3187
SseBI AGGCCT 1 cut(s) 3372
SspI AATATT 2 cut(s) 2395, 2646
SstI GAGCTC 3 cut(s) 833, 4690, 4719
StuI AGGCCT 1 cut(s) 3372
StyD4I CCNGG 9 cut(s) 177, 795, 1150, 3270, 3820, 4405, 4522, 4630, 4730
StyI CCWWGG 6 cut(s) 147, 2746, 3660, 3946, 4045, 4435
TaiI ACGT 8 cut(s) 419, 856, 1136, 1703, 2210, 2350, 3535, 5087
TaqII GACCGA 1 cut(s) 544
TatI WGTACW 7 cut(s) 390, 752, 1528, 2876, 3810, 4453, 4572
TauI GCSGC 5 cut(s) 521, 2744, 3878, 4800, 4867
TscAI CASTG 8 cut(s) 815, 1596, 2470, 3762, 4126, 4575, 4587, 4895
TseFI GTSAC 3 cut(s) 241, 737, 806
Tsp45I GTSAC 3 cut(s) 241, 737, 806
TspGWI ACGGA 3 cut(s) 736, 960, 2379
TspRI CASTG 8 cut(s) 815, 1596, 2470, 3762, 4126, 4575, 4587, 4895
Van91I CCANNNNNTGG 4 cut(s) 2960, 2989, 3913, 4121
Vha464I CTTAAG 1 cut(s) 4759
VpaK11BI GGWCC 1 cut(s) 3264
VspI ATTAAT 1 cut(s) 615
XapI RAATTY 9 cut(s) 345, 910, 1975, 2138, 2244, 3994, 4169, 4201, 4659
XbaI TCTAGA 1 cut(s) 4682
XceI RCATGY 5 cut(s) 339, 1030, 1209, 2768, 5048
XcmI CCANNNNNNNNNTGG 1 cut(s) 2564
XhoI CTCGAG 2 cut(s) 495, 5113
XmaJI CCTAGG 1 cut(s) 3946
XmiI GTMKAC 2 cut(s) 903, 3010
XmnI GAANNNNTTC 2 cut(s) 768, 3699
ZraI GACGTC 1 cut(s) 417
ZrmI AGTACT 1 cut(s) 2878
Zsp2I ATGCAT 5 cut(s) 1211, 2760, 2797, 3340, 4391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.