Rw4G030790

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006265
Physical Location & Seq
Forward (+)
57535767 .. 57546883
11117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030790.1

Sequence Viewer

Length: 5097 bp
ATGCTACTACTCACAAACTCTCTCTCTTTCCTACACTCCAACACAAGGTCTCTCCCTCACACCAAACCCTTGCCGCCGCCGCCGCCTTTCCCGTTCCTCCACCCAAACTCCCGCCATCTCCGCCTCCCTCCTAACTCCAAGGTCGTTCGAGGCGTGAGATTCGACGGCCCGGCCATCGATATCAGCCAAGTGACGCACACCGACGACGGCGATTTGATAATCGAGACTTGCCTCACGCGGAACTTGACTCCGGCGTTGTCTATTGAGCAAGGGCTTGAGAGTATCAAGGAGGCGGTTGAGGAGTTGAAGCTCCATCCTCCTTCAACTTGTAGTGGAATTCTCAGGTTTCAGGTGGCTGTGCCGCCGAGTGCGAAAGCCTTGAACTGGTTTTGCTGCCAACCGGAGTCGTCTGCGGTCTATCCACTGTTTTTTGTTTCCAAGGAAACTGAAAATCCGAGCTATAAATCATTGTATGTGAATGAAACTCGAGGAGTTTTCGGAATCGGTGCTGCAATTTACTTCACTCCTTCTACTAATTCTTCTTCTTCGATCAAAAGAATTCTATCAAATGAATCAGCTTTTGTAATAGCTTATGGTTTTATGGATATTAATTACGACCAAGAGTCGTCTTATATGAAGCATGAGCCAGGTTCATATTACTTTTTTATTCCTCAGATTGAGTTACATGAGCATGAGGGCGCTTCTGTATTAGCAGCAACTATTGGTTGGAGTGAGTCTTCCCTTTGTACATTTGAGGAAGCTGTTCTTTCTTATGAGTTATGTTTCAACCAGGTTACCTGTCAAGTTTGTTCCACAACAAAGAAATACCACACCACAAATATAAGATCTACTCTTAGGAAGCTCAATCTTCTGGAGGATGGAATGGTTTCAATGGTACACATGAACATGCTGTTAGCAGGTGGAAAAAACATTTCATCTGACATCATGGCACTGAAAGAGGCTCCGTCTTCCTCCCAGTTTTGTGTGAGGCTATCACCTACCATGGCTGTTGCTAATAAAATGTTGGATCATGCCAGTAAAATGTGCTTCTCAGTGCAAGATTGTGGCAACATCAACACTGTTTGGGCATCACTTATAGTTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCCCTAGCTGTTGCTGCATCCACTCATCCACTTATTACATGTATTGTATGTTATGATGAACGCTCACTTGCATTTCATGCTGTTGGGTATGCAAGAGGATCTGGCAAACCAGCAGTAGTCATAACATCATCTGGCACTGCAGTTTCAAATCTTCTTCCTGCTGTGGTTGAGGCTAGTCAAGATTTTGTACCACTTATATTGCTCACAGCTGATCGTCCTGCAGAACTTCAGGATGCTGGAGCAAACCAAGCAATCAATCAGGTAAACCATTTTGGCTCTTTTGCACGGTTTTTCTTTAGCCTTCCTGCACCCACAGATCATATTTCAGCTCGCATGGTGCTTACAACACTCGACAATGCTGTACATTGGGCAACCTCTTCACCATGTGGGCCTGTTCATATTAACTGTCCTTTCAGGGAGCCACTAGAAGATAGCCCTAGAAAATGGATGTTGAGTTGTTTAAATGGATTAAACCATTGGATGTCTAATTCTGAACCATTTACCAAGTATATTCAAGTACAACATGCTCATACAAGTAATGATGATTGTGGCGGAATGTCAGAAATTCTAAATGTAATTAAGGGAACTAACAAGGGTCTTCTACTTATCGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTCTTGGCTAAACACCTTCACTGGCCTGTTATAGCTGACATCTTGTCAGGATTACGATTGAGAAAGCTTTTGACTAGTTTCCCTGAAGTTGACAATGATTTATTGTTTGTTGATCATCTTGACCATGCCCTACTGTCAGATTATGTCAGTAAATGGATTAACATTGATGTGATTATTCAGATTGGGAGCAGGATTACAAGCAAGCGTATTGCTAAGATGCTGGAGGAATCCTTTCCCTGCTCTTATATCATGGTTGACAAGCATCCATTTCGTCACGATCCTTCTCATATTGTAACTCATAGAGTCCACAGTAACATTTTTGAATTTGCTGATTATATCCTTAAAGCTGAATTTCCCCATCTCAGCAAGGAATGGAGCACTTATCTTCAAATGATAAATGCAATGGTTGAAAGGGAGTTGTCATTTCAAATTTCTGCTAGACACTCCTTGACAGAACCGCAAGTCGCATACCTAATTTCAGAAGCACTTTCAGCTGAATCTGCTCTTTTCATTGGGAACAGTATGGCAATACGTGATGCAGATATGTATGGGTGTGGTTGGTCAGAGTATACACGTAGTATTGTTGCCATGAATTATAAACTACCCTGTCAAATGATTCGGGTGGCTGGGAATAGAGGTGCAAGTGGTATTGATGGGTTACTCAGTACAGCTGTTGGCTTTGCTATAGGATGCAATAAACGAGTACTTTGCGTGATTGGGGATGTTTCCTTCCTCCATGATACAAATGGCTTAGCAATTGTGAACCAGAGGACGTTCCGGAAACCAATGACAATAGTTGTGATTAACAATCATGGTGGTGCAATATTCAGTCTCCTTCCTCTTGCAGATAGAGTCAGACCAAGAATACTAAACCAATATTTCTACACCTCTCACAACATATCAATCCGTGAGCTTTGTGTAGCACATGGTGTGATGCATCTACATGCGACGACAAAGTTGGATTTTGAAGAAGCATTATTCACATCTCAACATGGAGGGATGGATTGTGTCATTGAAGTTGAGAGCTGCATAGATACCAATGCCAGCTTTCACAGTACTTTAAAAACATTTGCATGCCAAGCAGCAGATCAAGTCATAAAGCCTTCTAGTCAAGACTCGATATTAGATGGCACTTTACTCTGCAGAGTCCATAGAATGGAGTACTCACTGTTTAGAATGCCACTGTGTGCTCCTCATACTATGGTTTCTGTTGATGGCGGTACCACCAGCTTCCACAGGGAAGGGTTTATTTTAACTTTGTATTTTGAAGATGGAAGCTTTGGGCTTGGCGAGGTTTCACCCCTAGACATCTACAAAGAAAACCTTCTAGATGTAGAAGAGCAACTGAGGTTTCTCATCCATATGATGAAAGGAGCTCATATCAGTTGCTTCCTTCCTCTGCTAAGGGGCTCGTTTTCATCTTGGATATGGTCCAACCTTGGAATCCTGCCATGTACGCTCTTTCCTAGTGTGAGATGTGGTTTGGAAATGGCAATTCTCAATGCCATAGCAAATAGAAAAGGTTCCAATTTATTAGGAATACTCCATCCCCAGATAGTTGGAGATATATTTAAATCGTCGTCAACAGTTCAGATTTGTGCCCTTGTTGATTCCAGTGGGACACCCACACAAGTAGCTGATGCTATTGCTACACTTGTTGAAGAAGGATTTACTGCAGTAAAGATTAAGGTAGCTCGTTGTGGGAGTCCCTTACAGGATGCTGCAGTTATACAAGAAGTAAGAAAAAAAGTTGGATACCATATTAAAATTCGTGCAGATGCCAATCGGAACTGGACCTATGAAGAAGCTATCCAGTTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATTGAGGAACCTGTTCAAATTGAAGAAGATATTATCAAGTTTTGCAAAGAAAGTGGGTTGCCTGTGGCACTGGATGAAACTATTGACAATATTGGAGAACATCCACTTATAAAGCTCTCAAAGTATGCCCATCCAGGAATAGTAGCTGTTGTCATCAAGCCAAGTGTTGTTGGTGGCTTTGAAAATGCAGCAATAATTGCCCAATGGGCCCAGCAGCACCAGAAAATGGCTGTTGTTAGTGCTGCATTTGAAAGCAGCCTAGGTTTATCAGCATATATTCAGTTTTCCTGTTATCTCAACCAGATGAATTCAGAGATTTGTACAATGATGAACTATCCATTAGCCTCATCCATAGCCCATGGTTTAGGAACTTACCGCTGGCTAAAAGAAGATTTGACGACTACTCCTCTCAAGATAAATCGTAATCCACGTAGTGGGTTCGTAGAAGCATCTGTTGCTGATTCTGATCGAGTTCTGCAGCAATTTCAAATCAATGGAAACATAATTCATAGAAAGGTTACTGGAGAGCAAGTTTGTATATACCAGTTGCCTTTAGATTCAAAGGGTTTATCTTGCTCCATCAAAATTCAGGAGATTGGACAAGGATATGATGATAATGTATTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATCATGAAGGCCTTATCAGGATGTGGAAGATGTGTTTCTATTGACCTTCCTGGTCATGGTGGAACCAAGATACAAGGTCATGGTGATAAGGATGCTGTACAGGCTTCAGGTTTGAGTGTTGAGGTAGTTGCCGATCTTTTATGTAAAGTGATTGAGCATATTACTCCTGGGAAGGTTACCCTTGTCGGATATTCTATGGGAGCCAGGATCGCCCTTTGCATGGCTCTGAAGCTTAATAAAAAAGTTAAGGGAGCTATTATCATATCTGGCAGCCCTGGATTAAATGATGGAATGGCAAGAAAAATTCGTAGAGCTGAAGATGACTCTAGAGCACGCTTCCTTGTTGCATATGGCTTAAAGCACTTTCTAGACACCTGGTATGCTGGAGAGCTTTGGAACAGCTTAAGGGAACACCCTCACTTTCATCAGATAGCCGCTGACCGCCTGCATCACGGAGATGTGCAGAGTCTTGCAAAAGTTCTGTCTGCTTTAAGTGTTGGGAGGCAGCTGCCATTGTGGGAAGACTTGAAGCACTGCAAAACGCCTCTTTTGCTCATTGTTGGAGAGAAAGATAAAAAATTCAAGACAATAGCTCAGGATATGTTCCTTGCGATTGGTAGTGGCAATGGCAAATTGACTGGAGATGATGGCGCACCCAACGACCTTTGTGAGATTGTTGAGATCCCAGATTGTGGACATGCTGCTCATCTGGAAAACCCTCTCCCTGTAATCAGGGCATTAAGGCAATTTGTGACGAAACTGAACTGA

Protein Analysis

1698

Amino Acids

187.11

Weight (kDa)

6.27

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 363 - 475 3.6e-22 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 541 - 774 1.6e-23 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 824 - 933 1.5e-06 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 1148 - 1323 5.8e-24 Enolase C-terminal domain-like
Abhydrolase_1 PF00561 1426 - 1543 1.3e-18 alpha/beta hydrolase fold
Hydrolase_4 PF12146 1426 - 1645 1.4e-11 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 1428 - 1688 4.6e-22 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2406, 3770
AarI CACCTGC 1 cut(s) 908
Acc36I ACCTGC 1 cut(s) 908
Acc65I GGTACC 1 cut(s) 3029
AccB1I GGYRCC 1 cut(s) 3029
AccB7I CCANNNNNTGG 4 cut(s) 2965, 3886, 4094, 5021
AccI GTMKAC 1 cut(s) 2378
AccII CGCG 1 cut(s) 238
AccIII TCCGGA 1 cut(s) 2586
AclWI GGATC 6 cut(s) 1035, 1156, 1267, 2081, 4544, 5005
AcoI YGGCCR 1 cut(s) 171
AcuI CTGAAG 5 cut(s) 1373, 1914, 4419, 4577, 4665
AdeI CACNNNGTG 4 cut(s) 1547, 2738, 2996, 4094
AfiI CCNNNNNNNGG 8 cut(s) 45, 384, 2965, 3886, 4094, 4385, 4549, 5021
AflII CTTAAG 1 cut(s) 4732
AflIII ACRYGT 2 cut(s) 1199, 2381
AhdI GACNNNNNGTC 2 cut(s) 622, 1852
AhlI ACTAGT 1 cut(s) 1883
AjnI CCWGG 9 cut(s) 646, 789, 1144, 3793, 4378, 4495, 4532, 4603, 4703
Alw21I GWGCWC 4 cut(s) 2189, 3001, 3187, 4663
Alw26I GTCTC 3 cut(s) 54, 218, 2645
AlwI GGATC 6 cut(s) 1035, 1156, 1267, 2081, 4544, 5005
Ama87I CYCGRG 1 cut(s) 486
Aor13HI TCCGGA 1 cut(s) 2586
AoxI GGCC 6 cut(s) 166, 171, 1550, 1832, 3867, 4338
ApaI GGGCCC 1 cut(s) 3871
ArsI GACNNNNNNTTYG 2 cut(s) 4796, 4828
AseI ATTAAT 1 cut(s) 609
Asp700I GAANNNNTTC 6 cut(s) 762, 886, 2040, 3132, 3331, 3672
Asp718I GGTACC 1 cut(s) 3029
AspA2I CCTAGG 1 cut(s) 3919
AspLEI GCGC 2 cut(s) 701, 4982
AspS9I GGNCC 6 cut(s) 167, 1550, 3240, 3603, 3867, 3868
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 5 cut(s) 987, 1533, 3099, 4325, 4424
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 2 cut(s) 3240, 3603
AvrII CCTAGG 1 cut(s) 3919
BaeGI GKGCMC 2 cut(s) 3412, 3871
BanI GGYRCC 1 cut(s) 3029
BanII GRGCYC 3 cut(s) 3187, 3221, 3871
BbsI GAAGAC 4 cut(s) 729, 960, 1751, 4857
Bbv12I GWGCWC 4 cut(s) 2189, 3001, 3187, 4663
BceAI ACGGC 2 cut(s) 181, 223
BcgI CGANNNNNNTGC 4 cut(s) 2060, 2094, 2499, 2533
BciT130I CCWGG 9 cut(s) 648, 791, 1146, 3795, 4380, 4497, 4534, 4605, 4705
BciVI GTATCC 1 cut(s) 3557
BclI TGATCA 1 cut(s) 1921
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 3 cut(s) 54, 218, 2645
BcuI ACTAGT 1 cut(s) 1883
BfmI CTRYAG 8 cut(s) 1299, 1380, 2493, 2950, 3483, 3531, 3653, 4136
BfoI RGCGCY 1 cut(s) 702
BfrI CTTAAG 1 cut(s) 4732
BfuAI ACCTGC 1 cut(s) 908
BfuI GTATCC 1 cut(s) 3557
BglI GCCNNNNNGGC 1 cut(s) 3866
BglII AGATCT 1 cut(s) 845
BlnI CCTAGG 1 cut(s) 3919
BlpI GCTNAGC 1 cut(s) 2560
BmcAI AGTACT 3 cut(s) 2514, 2866, 2972
Bme18I GGWCC 2 cut(s) 3240, 3603
BmeRI GACNNNNNGTC 2 cut(s) 622, 1852
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 6 cut(s) 167, 1550, 3240, 3603, 3867, 3868
BmiI GGNNCC 9 cut(s) 963, 1581, 3031, 3334, 3631, 3669, 3869, 4393, 4531
BmrI ACTGGG 1 cut(s) 970
BmuI ACTGGG 1 cut(s) 970
BpiI GAAGAC 4 cut(s) 729, 960, 1751, 4857
BpmI CTGGAG 7 cut(s) 893, 1128, 1419, 2051, 4203, 4734, 4989
Bpu10I CCTNAGC 2 cut(s) 3212, 4923
Bpu1102I GCTNAGC 1 cut(s) 2560
BpuEI CTTGAG 2 cut(s) 296, 4055
BpuMI CCSGG 1 cut(s) 170
Bsa29I ATCGAT 1 cut(s) 177
BsaAI YACGTR 3 cut(s) 2342, 2384, 4091
BsaBI GATNNNNATC 5 cut(s) 1153, 3591, 3648, 4125, 4328
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 9 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018, 4496, 4603
BsaWI WCCGGW 2 cut(s) 400, 2586
BsaXI ACNNNNNCTCC 6 cut(s) 92, 122, 4048, 4078, 4176, 4206
Bsc4I CCNNNNNNNGG 8 cut(s) 45, 384, 2965, 3886, 4094, 4385, 4549, 5021
Bse3DI GCAATG 2 cut(s) 2217, 4960
Bse8I GATNNNNATC 5 cut(s) 1153, 3591, 3648, 4125, 4328
BseAI TCCGGA 1 cut(s) 2586
BseBI CCWGG 9 cut(s) 648, 791, 1146, 3795, 4380, 4497, 4534, 4605, 4705
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 9 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018, 4496, 4603
BseJI GATNNNNATC 5 cut(s) 1153, 3591, 3648, 4125, 4328
BseLI CCNNNNNNNGG 8 cut(s) 45, 384, 2965, 3886, 4094, 4385, 4549, 5021
BseMI GCAATG 2 cut(s) 2217, 4960
BseMII CTCAG 7 cut(s) 355, 686, 1065, 2185, 2485, 3146, 4937
BseRI GAGGAG 4 cut(s) 314, 504, 2991, 4056
BseSI GKGCMC 2 cut(s) 3412, 3871
BseYI CCCAGC 2 cut(s) 2435, 3870
BsgI GTGCAG 3 cut(s) 1452, 3603, 4811
Bsh1236I CGCG 1 cut(s) 238
BshFI GGCC 6 cut(s) 168, 173, 1552, 1834, 3869, 4340
BshNI GGYRCC 1 cut(s) 3029
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 4 cut(s) 2189, 3001, 3187, 4663
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 4 cut(s) 170, 251, 401, 2587
BslFI GGGAC 2 cut(s) 3442, 3501
BslI CCNNNNNNNGG 8 cut(s) 45, 384, 2965, 3886, 4094, 4385, 4549, 5021
BsmAI GTCTC 3 cut(s) 54, 218, 2645
BsmFI GGGAC 2 cut(s) 3442, 3501
BsmI GAATGC 1 cut(s) 2991
BsnI GGCC 6 cut(s) 168, 173, 1552, 1834, 3869, 4340
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 486
Bsp120I GGGCCC 1 cut(s) 3867
Bsp1286I GDGCHC 7 cut(s) 2189, 3001, 3187, 3221, 3412, 3871, 4663
Bsp13I TCCGGA 1 cut(s) 2586
Bsp1407I TGTACA 4 cut(s) 746, 1522, 3980, 4426
Bsp1720I GCTNAGC 1 cut(s) 2560
Bsp19I CCATGG 2 cut(s) 1002, 4018
BspANI GGCC 6 cut(s) 168, 173, 1552, 1834, 3869, 4340
BspCNI CTCAG 7 cut(s) 354, 685, 1064, 2184, 2484, 3147, 4936
BspDI ATCGAT 1 cut(s) 177
BspEI TCCGGA 1 cut(s) 2586
BspFNI CGCG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 4332
BspLI GGNNCC 9 cut(s) 963, 1581, 3031, 3334, 3631, 3669, 3869, 4393, 4531
BspMAI CTGCAG 7 cut(s) 1303, 1384, 2954, 3487, 3535, 3657, 4140
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 6 cut(s) 1035, 1156, 1267, 2081, 4544, 5005
BspQI GCTCTTC 1 cut(s) 3141
BspT107I GGYRCC 1 cut(s) 3029
BspTI CTTAAG 1 cut(s) 4732
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 2217, 4960
BsrGI TGTACA 4 cut(s) 746, 1522, 3980, 4426
BssECI CCNNGG 9 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018, 4496, 4603
BssNAI GTATAC 1 cut(s) 2379
BssT1I CCWWGG 7 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018
Bst1107I GTATAC 1 cut(s) 2379
Bst2UI CCWGG 9 cut(s) 648, 791, 1146, 3795, 4380, 4497, 4534, 4605, 4705
Bst6I CTCTTC 2 cut(s) 1543, 3141
BstAFI CTTAAG 1 cut(s) 4732
BstAPI GCANNNNNTGC 3 cut(s) 1238, 3857, 4115
BstAUI TGTACA 4 cut(s) 746, 1522, 3980, 4426
BstBAI YACGTR 3 cut(s) 2342, 2384, 4091
BstC8I GCNNGC 7 cut(s) 1492, 2012, 2854, 2884, 4040, 4663, 4775
BstDSI CCRYGG 2 cut(s) 1002, 4018
BstEII GGTNACC 2 cut(s) 793, 4504
BstFNI CGCG 1 cut(s) 238
BstH2I RGCGCY 1 cut(s) 702
BstHHI GCGC 2 cut(s) 701, 4982
BstMAI GTCTC 3 cut(s) 54, 218, 2645
BstNI CCWGG 9 cut(s) 648, 791, 1146, 3795, 4380, 4497, 4534, 4605, 4705
BstNSI RCATGY 6 cut(s) 910, 1203, 1688, 2756, 2886, 5030
BstPI GGTNACC 2 cut(s) 793, 4504
BstSFI CTRYAG 8 cut(s) 1299, 1380, 2493, 2950, 3483, 3531, 3653, 4136
BstSLI GKGCMC 2 cut(s) 3412, 3871
BstUI CGCG 1 cut(s) 238
BstV2I GAAGAC 4 cut(s) 729, 960, 1751, 4857
BstX2I RGATCY 3 cut(s) 845, 1259, 5010
BstXI CCANNNNNNTGG 1 cut(s) 3368
BstYI RGATCY 3 cut(s) 845, 1259, 5010
BstZ17I GTATAC 1 cut(s) 2379
Bsu15I ATCGAT 1 cut(s) 177
BsuI GTATCC 1 cut(s) 3557
BsuRI GGCC 6 cut(s) 168, 173, 1552, 1834, 3869, 4340
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 1002, 4018
BtsI GCAGTG 2 cut(s) 1296, 4861
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 7 cut(s) 1492, 2012, 2854, 2884, 4040, 4663, 4775
CciI TCATGA 1 cut(s) 4332
CfoI GCGC 2 cut(s) 701, 4982
Cfr13I GGNCC 6 cut(s) 167, 1550, 3240, 3603, 3867, 3868
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 202
CsiI ACCWGGT 2 cut(s) 789, 4703
CspCI CAANNNNNGTGG 6 cut(s) 1341, 1376, 2605, 2640, 3694, 3729
DraI TTTAAA 3 cut(s) 1623, 2871, 3382
DraIII CACNNNGTG 4 cut(s) 1547, 2738, 2996, 4094
DriI GACNNNNNGTC 2 cut(s) 622, 1852
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 2 cut(s) 1543, 3141
Eam1105I GACNNNNNGTC 2 cut(s) 622, 1852
EarI CTCTTC 2 cut(s) 1543, 3141
EciI GGCGGA 2 cut(s) 110, 1728
Ecl136II GAGCTC 1 cut(s) 3185
Eco130I CCWWGG 7 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018
Eco147I AGGCCT 1 cut(s) 4340
Eco24I GRGCYC 3 cut(s) 3187, 3221, 3871
Eco31I GGTCTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 2 cut(s) 3240, 3603
Eco53kI GAGCTC 1 cut(s) 3185
Eco57I CTGAAG 5 cut(s) 1373, 1914, 4419, 4577, 4665
Eco88I CYCGRG 1 cut(s) 486
Eco91I GGTNACC 2 cut(s) 793, 4504
EcoICRI GAGCTC 1 cut(s) 3185
EcoO65I GGTNACC 2 cut(s) 793, 4504
EcoRI GAATTC 3 cut(s) 336, 558, 3967
EcoRII CCWGG 9 cut(s) 646, 789, 1144, 3793, 4378, 4495, 4532, 4603, 4703
EcoRV GATATC 1 cut(s) 181
EcoT14I CCWWGG 7 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018
EcoT22I ATGCAT 1 cut(s) 2748
EcoT38I GRGCYC 3 cut(s) 3187, 3221, 3871
ErhI CCWWGG 7 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018
FalI AAGNNNNNCTT 4 cut(s) 750, 782, 3117, 3149
FaqI GGGAC 2 cut(s) 3442, 3501
FauI CCCGC 1 cut(s) 119
FauNDI CATATG 2 cut(s) 3171, 4678
FbaI TGATCA 1 cut(s) 1921
FblI GTMKAC 1 cut(s) 2378
FriOI GRGCYC 3 cut(s) 3187, 3221, 3871
GlaI GCGC 2 cut(s) 700, 4981
GsaI CCCAGC 2 cut(s) 2439, 3874
GsuI CTGGAG 7 cut(s) 893, 1128, 1419, 2051, 4203, 4734, 4989
HaeII RGCGCY 1 cut(s) 702
HaeIII GGCC 6 cut(s) 168, 173, 1552, 1834, 3869, 4340
HapII CCGG 4 cut(s) 170, 251, 401, 2587
HgaI GACGC 1 cut(s) 202
HhaI GCGC 2 cut(s) 701, 4982
Hin6I GCGC 2 cut(s) 699, 4980
HinP1I GCGC 2 cut(s) 699, 4980
HincII GTYRAC 3 cut(s) 1900, 2065, 3393
HindII GTYRAC 3 cut(s) 1900, 2065, 3393
HindIII AAGCTT 3 cut(s) 1874, 3085, 4559
HpaII CCGG 4 cut(s) 170, 251, 401, 2587
HphI GGTGA 5 cut(s) 987, 1533, 3099, 4325, 4424
Hpy166II GTNNAC 9 cut(s) 898, 1426, 1900, 2065, 2116, 2379, 2572, 3393, 5024
Hpy8I GTNNAC 9 cut(s) 898, 1426, 1900, 2065, 2116, 2379, 2572, 3393, 5024
Hpy99I CGWCG 5 cut(s) 167, 206, 209, 2761, 3391
HpyCH4IV ACGT 5 cut(s) 1127, 2341, 2383, 2582, 4090
HpySE526I ACGT 5 cut(s) 1127, 2341, 2383, 2582, 4090
HspAI GCGC 2 cut(s) 699, 4980
Kpn2I TCCGGA 1 cut(s) 2586
KpnI GGTACC 1 cut(s) 3033
Ksp22I TGATCA 1 cut(s) 1921
LguI GCTCTTC 1 cut(s) 3141
MabI ACCWGGT 2 cut(s) 789, 4703
MaeII ACGT 5 cut(s) 1127, 2341, 2383, 2582, 4090
MfeI CAATTG 1 cut(s) 2565
MflI RGATCY 3 cut(s) 845, 1259, 5010
MhlI GDGCHC 7 cut(s) 2189, 3001, 3187, 3221, 3412, 3871, 4663
MmeI TCCRAC 9 cut(s) 63, 707, 1005, 2748, 3267, 3349, 3541, 4495, 4870
Mph1103I ATGCAT 1 cut(s) 2748
MroI TCCGGA 1 cut(s) 2586
MroXI GAANNNNTTC 6 cut(s) 762, 886, 2040, 3132, 3331, 3672
MslI CAYNNNNRTG 8 cut(s) 690, 905, 1976, 2625, 2751, 2881, 3170, 4785
MspA1I CMGCKG 6 cut(s) 1370, 2303, 2480, 4038, 4766, 4837
MspCI CTTAAG 1 cut(s) 4732
MspI CCGG 4 cut(s) 170, 251, 401, 2587
MunI CAATTG 1 cut(s) 2565
Mva1269I GAATGC 1 cut(s) 2991
MvaI CCWGG 9 cut(s) 648, 791, 1146, 3795, 4380, 4497, 4534, 4605, 4705
MvnI CGCG 1 cut(s) 238
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 2 cut(s) 1002, 4018
NdeI CATATG 2 cut(s) 3171, 4678
NlaIV GGNNCC 9 cut(s) 963, 1581, 3031, 3334, 3631, 3669, 3869, 4393, 4531
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 3 cut(s) 190, 2081, 5080
NsiI ATGCAT 1 cut(s) 2748
NspI RCATGY 6 cut(s) 910, 1203, 1688, 2756, 2886, 5030
PaeI GCATGC 1 cut(s) 2886
PaeR7I CTCGAG 1 cut(s) 486
PagI TCATGA 1 cut(s) 4332
PaqCI CACCTGC 1 cut(s) 908
PceI AGGCCT 1 cut(s) 4340
PciI ACATGT 1 cut(s) 1199
PciSI GCTCTTC 1 cut(s) 3141
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 4087
PctI GAATGC 1 cut(s) 2991
PdmI GAANNNNTTC 6 cut(s) 762, 886, 2040, 3132, 3331, 3672
PflMI CCANNNNNTGG 4 cut(s) 2965, 3886, 4094, 5021
PfoI TCCNGGA 2 cut(s) 1144, 3793
Ppu21I YACGTR 3 cut(s) 2342, 2384, 4091
PscI ACATGT 1 cut(s) 1199
PshBI ATTAAT 1 cut(s) 609
PsiI TTATAA 2 cut(s) 2406, 3770
Psp124BI GAGCTC 1 cut(s) 3187
Psp6I CCWGG 9 cut(s) 646, 789, 1144, 3793, 4378, 4495, 4532, 4603, 4703
PspEI GGTNACC 2 cut(s) 793, 4504
PspFI CCCAGC 2 cut(s) 2435, 3870
PspGI CCWGG 9 cut(s) 646, 789, 1144, 3793, 4378, 4495, 4532, 4603, 4703
PspN4I GGNNCC 9 cut(s) 963, 1581, 3031, 3334, 3631, 3669, 3869, 4393, 4531
PspOMI GGGCCC 1 cut(s) 3867
PspPI GGNCC 6 cut(s) 167, 1550, 3240, 3603, 3867, 3868
PspXI VCTCGAGB 1 cut(s) 486
PstI CTGCAG 7 cut(s) 1303, 1384, 2954, 3487, 3535, 3657, 4140
PsuI RGATCY 3 cut(s) 845, 1259, 5010
PvuII CAGCTG 4 cut(s) 1370, 2303, 2480, 4837
RseI CAYNNNNRTG 8 cut(s) 690, 905, 1976, 2625, 2751, 2881, 3170, 4785
SacI GAGCTC 1 cut(s) 3187
SapI GCTCTTC 1 cut(s) 3141
Sau96I GGNCC 6 cut(s) 167, 1550, 3240, 3603, 3867, 3868
ScaI AGTACT 3 cut(s) 2514, 2866, 2972
SduI GDGCHC 7 cut(s) 2189, 3001, 3187, 3221, 3412, 3871, 4663
SexAI ACCWGGT 2 cut(s) 789, 4703
SfcI CTRYAG 8 cut(s) 1299, 1380, 2493, 2950, 3483, 3531, 3653, 4136
Sfr274I CTCGAG 1 cut(s) 486
SinI GGWCC 2 cut(s) 3240, 3603
SlaI CTCGAG 1 cut(s) 486
SmiI ATTTAAAT 1 cut(s) 3382
SmiMI CAYNNNNRTG 8 cut(s) 690, 905, 1976, 2625, 2751, 2881, 3170, 4785
SmlI CTYRAG 4 cut(s) 275, 486, 4070, 4732
SmoI CTYRAG 4 cut(s) 275, 486, 4070, 4732
SpeI ACTAGT 1 cut(s) 1883
SphI GCATGC 1 cut(s) 2886
SseBI AGGCCT 1 cut(s) 4340
SspI AATATT 3 cut(s) 2634, 2687, 3751
SstI GAGCTC 1 cut(s) 3187
StuI AGGCCT 1 cut(s) 4340
StyI CCWWGG 7 cut(s) 138, 438, 1002, 3247, 3633, 3919, 4018
SwaI ATTTAAAT 1 cut(s) 3382
TaiI ACGT 5 cut(s) 1130, 2344, 2386, 2585, 4093
TatI WGTACW 9 cut(s) 746, 1522, 1678, 2474, 2512, 2864, 2970, 3980, 4426
TauI GCSGC 6 cut(s) 76, 79, 82, 85, 364, 4766
TseFI GTSAC 3 cut(s) 190, 2081, 5080
Tsp45I GTSAC 3 cut(s) 190, 2081, 5080
TspGWI ACGGA 3 cut(s) 954, 2705, 4797
Van91I CCANNNNNTGG 4 cut(s) 2965, 3886, 4094, 5021
Vha464I CTTAAG 1 cut(s) 4732
VpaK11BI GGWCC 2 cut(s) 3240, 3603
VspI ATTAAT 1 cut(s) 609
XbaI TCTAGA 3 cut(s) 3136, 4655, 4696
XceI RCATGY 6 cut(s) 910, 1203, 1688, 2756, 2886, 5030
XcmI CCANNNNNNNNNTGG 1 cut(s) 2552
XhoI CTCGAG 1 cut(s) 486
XmaJI CCTAGG 1 cut(s) 3919
XmiI GTMKAC 1 cut(s) 2378
XmnI GAANNNNTTC 6 cut(s) 762, 886, 2040, 3132, 3331, 3672
ZrmI AGTACT 3 cut(s) 2514, 2866, 2972
Zsp2I ATGCAT 1 cut(s) 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.