FvH4_4g28800

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006265
Physical Location & Seq
Forward (+)
29292647 .. 29303488
10842 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g28800.t3

Sequence Viewer

Length: 2847 bp
ATGGAGCACTTATCTTCAAATGTTAAATGCAGCGAAGCACTTTCAGCTGAGTCTGCTCTTTTCATTGGGAACAGTATGGCAATACGTGATGCAGATATGTATGGGCGTGGTTGGTCAGAGTGCACTAGTAGCACTGCTGTCACGAATTTAAAATCAAATCTACCCTGTCAAATGGTTCGGGTGGCTGGGAATAGAGGTGCAAGTGGTATTGATGGGTTACTCAGCACAGCTGTTGGCTTTGCTGTAGGATGCAATAAACGAGTACTTTGTGTGCTGGGAGATGTTTCCTTCCTCCATGATACAAATGGCTTAGCAATTGTGAACCAGAGGACATTGCGGAAACCAATGACGATCGTTGTGATTAACAATCATGGTGGTGCAATATTCAGCCTACTTCCTCTAGCAGATAGAGTCAAACCAAGAATACTAGATCAATATTTCTACACCTCTCACAATGTTTCAATCCGTGAGCTGTGTGCGGCACATGGTGTGATGCATCTGCATGCGAAGACAAAGTTGGATCTTGAAGATGCGTTATTCACATCTCAACAAGGCGGGATAGATTATGTGATTGAAGTTGAGAGCTGCATAGATACCAATGCGAGCTTTCACAGTACTTTGAGAAAGTTTGCCTGCCAAGCAGCAGATCAAGTCTTAACGCCTTCTAGTCAAGACTCAATATTAGATGGCACTTCATTCTGCAGAGTCCAAAGAATGGAGTACTCACTATTTAGAATGCCACTGTGTGCTCCTCATACTATGGTCTCTGTTGATGGCGGTGCCACTAGCTTCTACAGGGAAGGGTTTATTTTGACTTTGTATTTTGAAGATGGCAGCTTTGGGCTTGGCGAGGTTTCACCTCTAGATATATGCAAAGAAAACCTTCTAGATGTAGAAGAGCAACTGAGGTTTCTCATCCATACAATGAAAGGAGCTCATATCAGTTGCTTCCTTCCTCTGCTAAAGGGCTCGTTCTCATCTTGGATACGGTCCAACCTTGGAATCCTGCCATGTACGCTCTTTCCTAGCGTCAGATGTGGTTTGGAAATGGCTATTCTCAATGCCATAGCAACTAGACAAGGTTTCAATTTACTAGGCATACTCCTTGGCCAGAAAGGTGGAGATGTATCACAATCCTCATCCACAGTTCAGATTTGTGCCCTTGTTGATTCCAATAGGACGCCCACAGAAGTAGCTGATTCTATTGCTACACTTGTTGAAGAAGGATTTACTGCAGTAAAGATTAAGGTAGCTCGTTCTGGGAGTCCGTTACAGGATGCTGCTGTTATACAAGCCGTCAGAAAGAAAGTTGGATACCATATTAAAATTCGTGCAGATGCCAATCGGAACTGGACCTATGAAGAAGCTATCCAGTTTGGTTCCTTGGTGAAGGATTGTGATCTGCAGTACATTGAGGAGCCTGTTCAATTTGAAGAAGATATTATCAAGTTTTGCAAAGAAAGTGGGTTGCCTGTGGCACTGGATGAAACTATTGACAGTATTGGAGAACATCCACTTATAAAGCTCTCAAAGTACACCCATCCAGGAATAGTAGCTGTTGTAATCAAGCCAAGTGTTGTTGGCGGCTTTGAAAATGCAGCAATAATTGCGCAATGGGCCCAGCAGCACCAGAAAATGGCTGTTATTAGTGCTGCATTTGAAAGCAGCCTAGGTTTGTCAGCATATATTCAGTTCTCCTGTTATCTCAACCAGAAGAATTCTGAGATTTGTACAATGATGAACTATCCATTAGCCTCATCCATAGCCCATGGTTTAGGAACTTACCGCTGGCTAAAAGAAGATGTGACAACTAGGCCTCTCAAGATAAATCGTAATCCACGTAGTGGGTTTGTAGAAGCATCTGTTGCTGATGCTGATCGAGTTTCGAAGCAATTTCAAATCAATGGGAACACAAGTCGTAGAAATTTTACGGGAGAGCAAGTTTGTGTTTACCAGATGCCTTTAGATTCAAAGGGTTTGTCTTGCTCCATCAAAATTCAGGAGATTGGACAAAGATACAATGATAATGTACTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATCATGAAGGCCATATCAGGATGTGGAAAATGCATTTCTATTGACCTTCCTGGTCATGGTGGAACAAAGATACAAAGTCATGGTGTTCGGGATGCTGTACAAGCTTCAGGTTTGTGTGTTGAGGTAGTTGCCGATCTTTTATGTAAAGTGATTGAGCATATCACTCCTGGGAAGGTTACCCTTGTAGGATATTCTATGGGAGCCAGGATTGCACTGTACATGGCTCTGAGGCTTCCTAATAAAGTTAAGGGAGCTATCATCATATCTGGCAGCCCAGGATTAAAAGATGAAGTGGCAAGAAAAGTTCGTAGAGCTGAAGATGATTCCAGAGCATGCTTCCTTGCTGCATATGGTTTAAAGCTCTTTCTAGACACTTGGTATGCTGGAGCGCTTTGGAACAGCTTAAGAGAACACCCGCACTTTCATCAGATAGTCGCCAACCGCCTGCACCATGGAAATGTGGAGAGTCTTGCAGAAGTTCTGTCTGCTCTAAGTGTTGGGAGGCAGCTGCCATTGTGGGAAGACTTGAAGCACTGCAAAACGCCTCTTTTACTAATTGTCGGAGAGAGAGATGAAAAGTTCAAGACAATAGCTCAGGATATGTCACTTGTGATTGGTAATGGTGATGGCAAATTGACCGGAGATGATGGCGCACCTAATGAACTTTGTGAGATAGTTGAGATCCCAGATTGTGGACATGCTGCTCATCTGGAAAATCCTCTCCCTGTAATCAGGGCATTAAGGCGATTTGTGTCGAAACTGAACTGA

Protein Analysis

949

Amino Acids

103.54

Weight (kDa)

6.56

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_C PF02775 52 - 161 2.7e-07 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 376 - 553 1.2e-25 Enolase C-terminal domain-like
Hydrolase_4 PF12146 653 - 863 1.4e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 654 - 767 1.5e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 656 - 916 7.5e-20 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1520
Acc16I TGCGCA 1 cut(s) 1611
AccB1I GGYRCC 1 cut(s) 779
AccB7I CCANNNNNTGG 4 cut(s) 715, 1636, 1844, 2771
AciI CCGC 8 cut(s) 337, 479, 555, 777, 1584, 1786, 2495, 2521
AclWI GGATC 2 cut(s) 528, 2755
AcoI YGGCCR 1 cut(s) 1108
AcsI RAATTY 5 cut(s) 145, 1326, 1717, 1924, 1995
AcuI CTGAAG 2 cut(s) 2169, 2415
AcyI GRCGYC 1 cut(s) 1181
AdeI CACNNNGTG 3 cut(s) 488, 746, 1844
AfeI AGCGCT 1 cut(s) 2469
AfiI CCNNNNNNNGG 5 cut(s) 715, 1636, 1844, 2135, 2771
AflII CTTAAG 1 cut(s) 2482
AhlI ACTAGT 1 cut(s) 125
AjnI CCWGG 5 cut(s) 1543, 2128, 2245, 2282, 2353
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
Alw21I GWGCWC 4 cut(s) 9, 125, 751, 937
Alw26I GTCTC 1 cut(s) 769
Alw44I GTGCAC 1 cut(s) 121
AlwI GGATC 2 cut(s) 528, 2755
Aor51HI AGCGCT 1 cut(s) 2469
AoxI GGCC 4 cut(s) 1108, 1617, 1814, 2088
ApaI GGGCCC 1 cut(s) 1621
ApaLI GTGCAC 1 cut(s) 121
ApoI RAATTY 5 cut(s) 145, 1326, 1717, 1924, 1995
ArsI GACNNNNNNTTYG 2 cut(s) 1964, 1996
Asp700I GAANNNNTTC 1 cut(s) 882
AspA2I CCTAGG 1 cut(s) 1669
AspLEI GCGC 3 cut(s) 1612, 2470, 2732
AspS9I GGNCC 4 cut(s) 990, 1353, 1617, 1618
AsuHPI GGTGA 4 cut(s) 849, 1399, 2075, 2714
AsuII TTCGAA 1 cut(s) 1886
AvaII GGWCC 2 cut(s) 990, 1353
AvrII CCTAGG 1 cut(s) 1669
BaeGI GKGCMC 3 cut(s) 125, 1162, 1621
BalI TGGCCA 1 cut(s) 1110
BanI GGYRCC 1 cut(s) 779
BanII GRGCYC 3 cut(s) 937, 971, 1621
BbsI GAAGAC 2 cut(s) 515, 2607
Bbv12I GWGCWC 4 cut(s) 9, 125, 751, 937
BccI CCATC 8 cut(s) 206, 680, 767, 824, 1548, 1997, 2699, 2720
BceAI ACGGC 1 cut(s) 1280
BciT130I CCWGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
BciVI GTATCC 2 cut(s) 978, 1307
BcoDI GTCTC 1 cut(s) 769
BcuI ACTAGT 1 cut(s) 125
BfmI CTRYAG 5 cut(s) 243, 700, 793, 1233, 1403
BfoI RGCGCY 1 cut(s) 2471
BfrI CTTAAG 1 cut(s) 2482
BfuI GTATCC 2 cut(s) 978, 1307
BlnI CCTAGG 1 cut(s) 1669
BlpI GCTNAGC 1 cut(s) 310
BmcAI AGTACT 3 cut(s) 264, 616, 722
Bme1390I CCNGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
Bme18I GGWCC 2 cut(s) 990, 1353
BmgT120I GGNCC 4 cut(s) 990, 1353, 1617, 1618
BmiI GGNNCC 5 cut(s) 781, 1381, 1419, 1619, 2281
BmrFI CCNGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
BpiI GAAGAC 2 cut(s) 515, 2607
BpmI CTGGAG 1 cut(s) 2484
Bpu10I CCTNAGC 1 cut(s) 2673
Bpu1102I GCTNAGC 1 cut(s) 310
Bpu14I TTCGAA 1 cut(s) 1886
BpuEI CTTGAG 1 cut(s) 1805
BsaAI YACGTR 2 cut(s) 86, 1841
BsaBI GATNNNNATC 4 cut(s) 1341, 1398, 1875, 2078
BsaHI GRCGYC 1 cut(s) 1181
BsaI GGTCTC 1 cut(s) 769
BsaJI CCNNGG 8 cut(s) 997, 1105, 1383, 1669, 1768, 2246, 2353, 2530
BsaWI WCCGGW 1 cut(s) 2717
BsaXI ACNNNNNCTCC 2 cut(s) 1926, 1956
Bsc4I CCNNNNNNNGG 5 cut(s) 715, 1636, 1844, 2135, 2771
Bse1I ACTGG 4 cut(s) 1355, 1372, 1485, 2065
Bse3DI GCAATG 2 cut(s) 332, 1619
Bse8I GATNNNNATC 4 cut(s) 1341, 1398, 1875, 2078
BseBI CCWGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
BseDI CCNNGG 8 cut(s) 997, 1105, 1383, 1669, 1768, 2246, 2353, 2530
BseJI GATNNNNATC 4 cut(s) 1341, 1398, 1875, 2078
BseLI CCNNNNNNNGG 5 cut(s) 715, 1636, 1844, 2135, 2771
BseMI GCAATG 2 cut(s) 332, 1619
BseMII CTCAG 6 cut(s) 39, 235, 896, 1713, 2297, 2687
BseNI ACTGG 4 cut(s) 1355, 1372, 1485, 2065
BseRI GAGGAG 2 cut(s) 741, 1430
BseSI GKGCMC 3 cut(s) 125, 1162, 1621
BseYI CCCAGC 3 cut(s) 185, 274, 1620
BsgI GTGCAG 2 cut(s) 1353, 2510
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 4 cut(s) 1110, 1619, 1816, 2090
BshNI GGYRCC 1 cut(s) 779
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 4 cut(s) 9, 125, 751, 937
BsiSI CCGG 1 cut(s) 2718
BslI CCNNNNNNNGG 5 cut(s) 715, 1636, 1844, 2135, 2771
BsmAI GTCTC 1 cut(s) 769
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 4 cut(s) 1110, 1619, 1816, 2090
Bso31I GGTCTC 1 cut(s) 769
Bsp119I TTCGAA 1 cut(s) 1886
Bsp120I GGGCCC 1 cut(s) 1617
Bsp1286I GDGCHC 7 cut(s) 9, 125, 751, 937, 971, 1162, 1621
Bsp1407I TGTACA 3 cut(s) 1730, 2176, 2295
Bsp143I GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
Bsp1720I GCTNAGC 1 cut(s) 310
Bsp19I CCATGG 2 cut(s) 1768, 2530
BspACI CCGC 8 cut(s) 337, 479, 555, 777, 1584, 1786, 2495, 2521
BspANI GGCC 4 cut(s) 1110, 1619, 1816, 2090
BspCNI CTCAG 6 cut(s) 40, 234, 897, 1714, 2298, 2686
BspHI TCATGA 1 cut(s) 2082
BspLI GGNNCC 5 cut(s) 781, 1381, 1419, 1619, 2281
BspMAI CTGCAG 3 cut(s) 704, 1237, 1407
BspPI GGATC 2 cut(s) 528, 2755
BspQI GCTCTTC 1 cut(s) 891
BspT104I TTCGAA 1 cut(s) 1886
BspT107I GGYRCC 1 cut(s) 779
BspTI CTTAAG 1 cut(s) 2482
BspTNI GGTCTC 1 cut(s) 769
BsrDI GCAATG 2 cut(s) 332, 1619
BsrGI TGTACA 3 cut(s) 1730, 2176, 2295
BsrI ACTGG 4 cut(s) 1355, 1372, 1485, 2065
BssECI CCNNGG 8 cut(s) 997, 1105, 1383, 1669, 1768, 2246, 2353, 2530
BssMI GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
BssNI GRCGYC 1 cut(s) 1181
BssT1I CCWWGG 6 cut(s) 997, 1105, 1383, 1669, 1768, 2530
Bst2UI CCWGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
Bst4CI ACNGT 7 cut(s) 74, 614, 744, 990, 1147, 1499, 2295
Bst6I CTCTTC 1 cut(s) 891
BstACI GRCGYC 1 cut(s) 1181
BstAFI CTTAAG 1 cut(s) 2482
BstAPI GCANNNNNTGC 2 cut(s) 1607, 1865
BstAUI TGTACA 3 cut(s) 1730, 2176, 2295
BstBAI YACGTR 2 cut(s) 86, 1841
BstBI TTCGAA 1 cut(s) 1886
BstC8I GCNNGC 6 cut(s) 504, 604, 634, 1790, 2413, 2525
BstDEI CTNAG 8 cut(s) 48, 221, 310, 905, 1722, 2306, 2570, 2673
BstDSI CCRYGG 2 cut(s) 1768, 2530
BstEII GGTNACC 1 cut(s) 2254
BstH2I RGCGCY 1 cut(s) 2471
BstHHI GCGC 3 cut(s) 1612, 2470, 2732
BstKTI GATC 8 cut(s) 354, 433, 523, 649, 1402, 1879, 2215, 2763
BstMAI GTCTC 1 cut(s) 769
BstMBI GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
BstMCI CGRYCG 1 cut(s) 354
BstNI CCWGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
BstNSI RCATGY 3 cut(s) 506, 2415, 2780
BstPI GGTNACC 1 cut(s) 2254
BstSCI CCNGG 5 cut(s) 1543, 2128, 2245, 2282, 2353
BstSFI CTRYAG 5 cut(s) 243, 700, 793, 1233, 1403
BstSLI GKGCMC 3 cut(s) 125, 1162, 1621
BstV2I GAAGAC 2 cut(s) 515, 2607
BstX2I RGATCY 2 cut(s) 520, 2760
BstXI CCANNNNNNTGG 1 cut(s) 1118
BstYI RGATCY 2 cut(s) 520, 2760
BsuI GTATCC 2 cut(s) 978, 1307
BsuRI GGCC 4 cut(s) 1110, 1619, 1816, 2090
BtgI CCRYGG 2 cut(s) 1768, 2530
BtsI GCAGTG 2 cut(s) 132, 2611
BtsIMutI CAGTG 5 cut(s) 132, 740, 1478, 2291, 2611
Cac8I GCNNGC 6 cut(s) 504, 604, 634, 1790, 2413, 2525
CciI TCATGA 1 cut(s) 2082
CfoI GCGC 3 cut(s) 1612, 2470, 2732
Cfr13I GGNCC 4 cut(s) 990, 1353, 1617, 1618
CseI GACGC 2 cut(s) 1018, 1189
CspCI CAANNNNNGTGG 4 cut(s) 355, 390, 1444, 1479
DdeI CTNAG 8 cut(s) 48, 221, 310, 905, 1722, 2306, 2570, 2673
DpnI GATC 8 cut(s) 353, 432, 522, 648, 1401, 1878, 2214, 2762
DpnII GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
DraI TTTAAA 2 cut(s) 150, 2436
DraIII CACNNNGTG 3 cut(s) 488, 746, 1844
EaeI YGGCCR 1 cut(s) 1108
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
Ecl136II GAGCTC 1 cut(s) 935
Eco130I CCWWGG 6 cut(s) 997, 1105, 1383, 1669, 1768, 2530
Eco147I AGGCCT 1 cut(s) 1816
Eco24I GRGCYC 3 cut(s) 937, 971, 1621
Eco31I GGTCTC 1 cut(s) 769
Eco47I GGWCC 2 cut(s) 990, 1353
Eco47III AGCGCT 1 cut(s) 2469
Eco53kI GAGCTC 1 cut(s) 935
Eco57I CTGAAG 2 cut(s) 2169, 2415
Eco91I GGTNACC 1 cut(s) 2254
EcoICRI GAGCTC 1 cut(s) 935
EcoO65I GGTNACC 1 cut(s) 2254
EcoRI GAATTC 1 cut(s) 1717
EcoRII CCWGG 5 cut(s) 1543, 2128, 2245, 2282, 2353
EcoT14I CCWWGG 6 cut(s) 997, 1105, 1383, 1669, 1768, 2530
EcoT22I ATGCAT 2 cut(s) 498, 2114
EcoT38I GRGCYC 3 cut(s) 937, 971, 1621
ErhI CCWWGG 6 cut(s) 997, 1105, 1383, 1669, 1768, 2530
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FauI CCCGC 2 cut(s) 548, 2502
FauNDI CATATG 1 cut(s) 2428
FriOI GRGCYC 3 cut(s) 937, 971, 1621
FspI TGCGCA 1 cut(s) 1611
GlaI GCGC 3 cut(s) 1611, 2469, 2731
GsaI CCCAGC 3 cut(s) 189, 278, 1624
GsuI CTGGAG 1 cut(s) 2484
HaeII RGCGCY 1 cut(s) 2471
HaeIII GGCC 4 cut(s) 1110, 1619, 1816, 2090
HapII CCGG 1 cut(s) 2718
HgaI GACGC 2 cut(s) 1018, 1189
HhaI GCGC 3 cut(s) 1612, 2470, 2732
Hin1I GRCGYC 1 cut(s) 1181
Hin6I GCGC 3 cut(s) 1610, 2468, 2730
HinP1I GCGC 3 cut(s) 1610, 2468, 2730
HindIII AAGCTT 1 cut(s) 2181
HpaII CCGG 1 cut(s) 2718
HphI GGTGA 4 cut(s) 849, 1399, 2075, 2714
Hpy166II GTNNAC 5 cut(s) 123, 322, 1536, 1951, 2774
Hpy188I TCNGA 9 cut(s) 118, 1034, 1152, 1301, 1347, 1723, 2307, 2508, 2642
Hpy8I GTNNAC 5 cut(s) 123, 322, 1536, 1951, 2774
HpyCH4III ACNGT 7 cut(s) 74, 614, 744, 990, 1147, 1499, 2295
HpyCH4IV ACGT 2 cut(s) 85, 1840
HpyF3I CTNAG 8 cut(s) 48, 221, 310, 905, 1722, 2306, 2570, 2673
HpySE526I ACGT 2 cut(s) 85, 1840
Hsp92I GRCGYC 1 cut(s) 1181
HspAI GCGC 3 cut(s) 1610, 2468, 2730
Kzo9I GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 8 cut(s) 4, 754, 932, 1417, 1991, 2279, 2330, 2465
MaeII ACGT 2 cut(s) 85, 1840
MaeIII GTNAC 6 cut(s) 139, 216, 1269, 1804, 2254, 2682
MalI GATC 8 cut(s) 353, 432, 522, 648, 1401, 1878, 2214, 2762
MboI GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 2 cut(s) 520, 2760
MhlI GDGCHC 7 cut(s) 9, 125, 751, 937, 971, 1162, 1621
MlsI TGGCCA 1 cut(s) 1110
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 6 cut(s) 59, 420, 668, 714, 1273, 2554
MmeI TCCRAC 4 cut(s) 498, 1017, 1291, 2620
Mox20I TGGCCA 1 cut(s) 1110
Mph1103I ATGCAT 2 cut(s) 498, 2114
MroXI GAANNNNTTC 1 cut(s) 882
MscI TGGCCA 1 cut(s) 1110
MslI CAYNNNNRTG 3 cut(s) 375, 501, 2535
Msp20I TGGCCA 1 cut(s) 1110
MspA1I CMGCKG 4 cut(s) 47, 230, 1788, 2587
MspCI CTTAAG 1 cut(s) 2482
MspI CCGG 1 cut(s) 2718
MspR9I CCNGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
NcoI CCATGG 2 cut(s) 1768, 2530
NdeI CATATG 1 cut(s) 2428
NdeII GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
NlaIV GGNNCC 5 cut(s) 781, 1381, 1419, 1619, 2281
NmuCI GTSAC 3 cut(s) 139, 1804, 2682
NsbI TGCGCA 1 cut(s) 1611
NsiI ATGCAT 2 cut(s) 498, 2114
NspI RCATGY 3 cut(s) 506, 2415, 2780
NspV TTCGAA 1 cut(s) 1886
PaeI GCATGC 2 cut(s) 506, 2415
PagI TCATGA 1 cut(s) 2082
PceI AGGCCT 1 cut(s) 1816
PciSI GCTCTTC 1 cut(s) 891
PcsI WCGNNNNNNNCGW 1 cut(s) 1837
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 5 cut(s) 1002, 1169, 1199, 1967, 2402
PflMI CCANNNNNTGG 4 cut(s) 715, 1636, 1844, 2771
PfoI TCCNGGA 1 cut(s) 1543
Ple19I CGATCG 1 cut(s) 354
PleI GAGTC 6 cut(s) 58, 419, 668, 713, 1272, 2553
PpsI GAGTC 6 cut(s) 58, 419, 668, 713, 1272, 2553
Ppu21I YACGTR 2 cut(s) 86, 1841
PsiI TTATAA 1 cut(s) 1520
Psp124BI GAGCTC 1 cut(s) 937
Psp6I CCWGG 5 cut(s) 1543, 2128, 2245, 2282, 2353
PspEI GGTNACC 1 cut(s) 2254
PspFI CCCAGC 3 cut(s) 185, 274, 1620
PspGI CCWGG 5 cut(s) 1543, 2128, 2245, 2282, 2353
PspN4I GGNNCC 5 cut(s) 781, 1381, 1419, 1619, 2281
PspOMI GGGCCC 1 cut(s) 1617
PspPI GGNCC 4 cut(s) 990, 1353, 1617, 1618
PstI CTGCAG 3 cut(s) 704, 1237, 1407
PsuI RGATCY 2 cut(s) 520, 2760
PvuI CGATCG 1 cut(s) 354
PvuII CAGCTG 3 cut(s) 47, 230, 2587
RseI CAYNNNNRTG 3 cut(s) 375, 501, 2535
SacI GAGCTC 1 cut(s) 937
SapI GCTCTTC 1 cut(s) 891
Sau3AI GATC 8 cut(s) 351, 430, 520, 646, 1399, 1876, 2212, 2760
Sau96I GGNCC 4 cut(s) 990, 1353, 1617, 1618
ScaI AGTACT 3 cut(s) 264, 616, 722
SchI GAGTC 6 cut(s) 59, 420, 668, 714, 1273, 2554
ScrFI CCNGG 5 cut(s) 1545, 2130, 2247, 2284, 2355
SduI GDGCHC 7 cut(s) 9, 125, 751, 937, 971, 1162, 1621
SfcI CTRYAG 5 cut(s) 243, 700, 793, 1233, 1403
SfuI TTCGAA 1 cut(s) 1886
SinI GGWCC 2 cut(s) 990, 1353
SmiMI CAYNNNNRTG 3 cut(s) 375, 501, 2535
SmlI CTYRAG 2 cut(s) 1820, 2482
SmoI CTYRAG 2 cut(s) 1820, 2482
SpeI ACTAGT 1 cut(s) 125
SphI GCATGC 2 cut(s) 506, 2415
SseBI AGGCCT 1 cut(s) 1816
SsiI CCGC 8 cut(s) 337, 479, 555, 777, 1584, 1786, 2495, 2521
SspI AATATT 3 cut(s) 384, 437, 681
SstI GAGCTC 1 cut(s) 937
StuI AGGCCT 1 cut(s) 1816
StyD4I CCNGG 5 cut(s) 1543, 2128, 2245, 2282, 2353
StyI CCWWGG 6 cut(s) 997, 1105, 1383, 1669, 1768, 2530
TaaI ACNGT 7 cut(s) 74, 614, 744, 990, 1147, 1499, 2295
TaiI ACGT 2 cut(s) 88, 1843
TaqI TCGA 3 cut(s) 1879, 1886, 2834
TatI WGTACW 9 cut(s) 262, 614, 720, 1407, 1533, 1730, 2029, 2176, 2295
TauI GCSGC 2 cut(s) 482, 1587
TfiI GAWTC 5 cut(s) 1002, 1169, 1199, 1967, 2402
TscAI CASTG 5 cut(s) 139, 747, 1485, 2298, 2618
TseFI GTSAC 3 cut(s) 139, 1804, 2682
Tsp45I GTSAC 3 cut(s) 139, 1804, 2682
TspGWI ACGGA 2 cut(s) 455, 1257
TspRI CASTG 5 cut(s) 139, 747, 1485, 2298, 2618
Van91I CCANNNNNTGG 4 cut(s) 715, 1636, 1844, 2771
Vha464I CTTAAG 1 cut(s) 2482
VneI GTGCAC 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 990, 1353
XapI RAATTY 5 cut(s) 145, 1326, 1717, 1924, 1995
XbaI TCTAGA 3 cut(s) 862, 886, 2446
XceI RCATGY 3 cut(s) 506, 2415, 2780
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XmaJI CCTAGG 1 cut(s) 1669
XmnI GAANNNNTTC 1 cut(s) 882
ZrmI AGTACT 3 cut(s) 264, 616, 722
Zsp2I ATGCAT 2 cut(s) 498, 2114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.