Rh4BG360800

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0006265
Physical Location & Seq
Forward (+)
52003586 .. 52015202
11617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG360800.1

Sequence Viewer

Length: 5103 bp
ATGCTACTACTCACAAACTCTCTCTCTTTCCTACACTCCAACACAAGGTCTCTCCCTCACACCAAACCCTCGCCGCCGCCGCCGCCGCCTTTCCCGTTCCTCCACCCAAACTCCCGCCATCTCCGCCTCCCTCCTAACTCCAAGGTCGTTCGAGGCGTGAGATTCGACGGCCCGGCCATCGATATCAGCCAAGTGACGGACACCGACGACGGCGATTTGATAATCGAGACTTGCCTCACGCGGAACTTGACTCCGGCGTTGTCTATTGAGCAAGGGCTTCAGAGGATCAAGGAGGCGGTTGAGGAGTTGAAGCTCAATCCTCCTTCAACTTGTAGTGGAATTCTCAGGTTTCAGGTGGCTGTGCCGCCGAGTGCGAAAGCCTTGAACTGGTTTTGCTGCCAACCGGAGTCGTCTGCGGTCTATCCACTGTTTTTTGTTTCCAAGGAAACTGAAAATCCGAGCTATAAATCACTGTATGTGAATGAAACTCGAGGAGTTTTCGGAATCGGTGCTGCAATTTACTTCACGCCTTCTTCTTCTTCTTCTTCTTCGATCAAAAGAATTCTATCAAATGAATCAGCTTTTGTAATAGCTTATGGTTTTATGGATATTAATTACGACCAAGAGTCATCTTATATGAAGCATGAGGCAGGTTCATATTACTTTTTTATTCCTCAGATTGAGTTACATGAGCATGAGGGCGCTTCTGTATTAGCAGCAACTATTGGTTGGAGTGAGTCTTCCCTTTGTACATTTGAGGAAGCTGTTCTTTCTTATGAGTTATGTTTCAACCAGGTTACCTGTCAAGTTTGTTCCACAACAAAGAAATCCCTCACCACAAATATAAGATCTACTCTTAGGAAGCTCAAGCTTCTGGAGGATGGAATGGTTTCTATGGTACACATGAACATGCTGTTAGCAGGTGGAAAAAACATTTCGTCTGACATCATGGCACTGAAAGAGGCTCCGTCTTCCTCCCAGTTTTGTGTGAGGCTTTCACCTACCATGGCTGTTGCTAATAAAATGTTGGATCATGCCAGTAAAATGTGCTTCTCAGTGCAAGATTGTGGCAACATCAACACTGTTTGGGCATCACTTATAGTTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCCCTTGCTGTTGCTGCATCCACTCATCCACTTATTACATGTATTGTATGTTATGATGAACGCTCACTTGCATTTCATGCTGTTGGGTATGCAAGAGGATCTGACAAACCAGCAGTAGTCATAACATCATCTGGCACAGCAGTTTCAAATCTTCTTCCTGCTGTGGTCGAGGCTAGTCAAGATTTTGTACCACTTATATTGCTCACAGCTGATCGTCCTGCAGAACTGCAGGATGCTGGAGCAAACCAAGCAATCAATCAGGTAAACCATTTTGGCTCTTTTGCACGGTTTTTCTTTAGCCTTCCTGCACCCACAGATCATATTTCAGCTCGCATGGTGCTTACAACACTCGACAATGCTGTACATTGGGCAACCTCTTCACCATGTGGGCCTGCTCATATTAACTGTCCTTTCAGGGAGCCACTAGAAGATAGCCCTAGAAAATGGATGTTGAGTTGTTTGAACGGATTAAACCATTGGATGTCTAATTCTGAACCATTTACCAAGTATATTCAAGTACAACATGCTCATGCAAGTAATGATGGTTGTGGCGGAATGTCAGAAATTCTAAATGTAATTAAGGGAACTAACAAGGGTCTTCTACTTATCGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTCTTGGCTAAACACCTTCACTGGCCTGTTATAGCTGACATCTTGTCAGGATTACGATTGAGAAAGCTTTTGACTAGTTTCCCTGGAGTTGACAATGATTTATTGTTTGTTGATCATCTTGATCATGCCCTACTGTCAGATTCTGTCAGTAAATGGATTAACATTGATGTGATTATTCAGATTGGGAGCAGGATTACAAGCAAGCGTATTGCTAAGATGCTAGAGGAATCCTTTCCCTGCTCATATATCATGGTTGACAAGCATCCATTTCGTCACGATCCTTCTCATATTGTAACTCATAGAGTCCACAGTAACATTTTTGAATTTGCTGATTATATCCTTAAAGCTGAATTTCCCCATCTCAGCAAGGAATGGAGCACTTATCTTCAAATGATAAATGCAATGGTTGAAAGGGAGTTGTCATTTCAAATTTCTGCTAGACACTCCTTGACAGAACCTCAAGTCGCATACCTAATTTCAGAAGCACTTTCAGCTGAATCTGCTCTTTTCATTGGGAACAGTATGGCAATACGTGATGCAGATATGTATGGGCGTGGTTGGTCAGAGTATACACGTAGTATTGTTGCCATGAATTATAAACTACCCTGTCAAATGATTCGGGTGGCTGGGAATAGAGGTGCAAGTGGCATTGATGGGTTACTCAGTACAGCTGTTGGCTTTGCTATAGGATGCAATAAACGAGTACTTTGCGTGATTGGAGATGTTTCCTTCCTCCATGATACAAATGGCTTAGCAATTGTGAACCAGAGGACGTTCCGGAAACCAATGACGATAGTTGTGATTAACAATCATGGTGGTGCAATATTCAGTCTCCTTCCTCTTGCAGATAGAGTCAAACCAAGAATACTAAACCAATATTTCTACACCTCTCACAACATATCAATCCGTGAGCTTTGTGTAGCACATGGTGTGATGCATCTACATGCGAAGACAAAGTTGGATTTTGAAGATGCGTTATTCACATCCCAACATGGAGGGATGGATTGTGTCATTGAAGTTGAGAGCTGCATAGATACCAATGCCAGCTTTCACAGTACTTTAAGAACATTTGCATGCCAAGCAGCAGATCAAGTCATAAAGCCTTCTAGTCAAGACTCAATATTAGATGACACTTTACTCTGCAGAGTCCATAGAATGGAGTACTCACTGTTTAGAATGCCACTGTGTGCTCCTCATACTATGGTTTCTGTTGATGGCGGTACCACCAGCTTCCACAGGGAAGGGTTTATTTTAACTTTGTATTTTGAAGATGGAAGCTTTGGGCTTGGCGAGGTTTCACCCCTAGACATCTACAAAGAAAACCTTCTAGATGTAGAAGAGCAACTGAGGTTTCTCATCCATATGATGAAAGGAGCTCATATCAGTTGCTTCCTTCCTCTGCTAAAGGGCTCGTTTTCATCTTGGATATGGTCCAACCTTGGAATCCTGCCATGTACGCTCTTTCCTAGTGTGAGATGTGGTTTGGAAATGGCAATTCTCAATGCCATAGCAAATAGACAAGGTTCCAATTTATTAGGAATACTCCATCCCCAGATAGTTGGAGATATATTTAAATCGTCATCAACAGTTCAGATTTGTGCCCTTGTTGATTCCAGTGGGACACCCACACAAGTAGCTGATGCTATTGCTACACTTGTTGAAGAAGGATTTACTGCAGTAAAGATTAAGGTAGCTCGTTGTGGGAGTCCCTTACAGGATGCTGCAGTTATACAAGAAGTAAGAAAAAAAGTTGGATACCATATTAAAATTCGTGCAGATGCCAATCGGAACTGGACCTATGAAGAAGCTATCCAGTTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATCGAGGAACCTGTTCAAATTGAAGAAGATATTATCAAGTTTTGCAAAGAAAGTGGGTTGCCTGTGGCACTGGATGAAACTATTGACAATATTGGAGAACATCCACTTATAAAGCTCTCAAAGTATGCCCATCCAGGAATAGTAGCTGTTGTCATCAAGCCAAGTGTTGTTGGTGGCTTTGAAAATGCAGCAATAATTGCCCAATGGGCCCAGCAGCACCAGAAAATGGCTGTTGTTAGTGCAGCATTTGAAAGCAGCCTAGGTTTGTCAGCATATATTCAGTTTTCCTGTTATCTCAACCAGATGAATTCAGAGATTTGTACAATGATGAACTATCCATTAGCCTCATCCATAGCCCATGGTTTAGGAACTTACCGCTGGCTAAAAGAAGATTTGACGACTACTCCTCTCAAGATAAATCGTAATCCACGTAGTGGGTTCGTAGAAGCATCTGTTGCTGATTCTGATCGAGTTCTGCAGCAATTTCAAATCAATGGAAACATAATTCGTAGAAAGTTTACTGGAGAGCAAGTTTGTATATACCAGTTGCCTTTAGATTCAAAGGGTTTATCTTGCTCCATCAAAATTCAGGAGATTGGACAAGGATATGATGATAATGTATTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATCATGAAGGCCTTATCAGGATGTGGAAGATGTGTTTCTATTGACCTTCCTGGTCATGGTGGAACCAAGATACAAAGTCATGGTGATAAGGATGCTGTACAGGCTTCAGGTTTGTGTGTTGAGGTAGTTGCCGATCTTTTATGTAAAGTGATTGAGCATATTACTCCTGGGAAGGTTACCCTTGTTGGATATTCTATGGGTGCCAGGATCGCCCTTTGCATGGCTCTGAAGCTTACTAAAAAAGTTAAGGGAGCTATTATCATATCTGGCAGCCCTGGATTAAATGATGGAATGGCAAGAAAAATTCGTAGAGCTGAAGATGACTCTAGAGCACGCTTCCTTGCTGCATATGGCTTAAAGCACTTTCTAGACACCTGGTATGCTGGAGAGCTTTGGAACAGCAGCTTAAGGGAACACCCTCACTTTCATCAGATAGCCACCGACCGCCTGCATCATGGAGATGTGCAGAGTCTTGCAAAAGTTCTGTCTGCTTTAAGTGTTGGGAGGCAGCTGCCATTGTGGGAAGACTTGAAGCACTGCAAAACGCCTCTTTTGCTCATTGTTGGAGAGAAAGATAAAAAATTCAAAGCAATAGCTCAGGATATGTTCCTTGCGATTGGTAGTGGCAATGGCAAATTGACTGGAGATGATGGCACACCCAACGACCTTTGTGAGATTGTTGAGATCCCAGATTGTGGACATGCTGCTCATCTGGAAAACCCTCTCCCTGTAATCAGGGCATTAAGGCAATTTGTGACGAAACTGAACTGA

Protein Analysis

1700

Amino Acids

187.13

Weight (kDa)

6.34

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 364 - 476 2e-21 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 542 - 775 3e-23 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 825 - 934 2.3e-06 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 1149 - 1324 5.9e-24 Enolase C-terminal domain-like
Abhydrolase_1 PF00561 1427 - 1685 4.1e-20 alpha/beta hydrolase fold
Hydrolase_4 PF12146 1427 - 1647 4.3e-12 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 1429 - 1690 3.1e-22 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2409, 3773
AarI CACCTGC 1 cut(s) 911
Acc36I ACCTGC 2 cut(s) 641, 911
Acc65I GGTACC 1 cut(s) 3032
AccB1I GGYRCC 2 cut(s) 3032, 4532
AccB7I CCANNNNNTGG 4 cut(s) 2968, 3889, 4097, 5027
AccI GTMKAC 1 cut(s) 2381
AccII CGCG 1 cut(s) 241
AccIII TCCGGA 1 cut(s) 2589
AclWI GGATC 7 cut(s) 293, 1038, 1159, 1270, 2084, 4547, 5011
AcoI YGGCCR 1 cut(s) 174
AcuI CTGAAG 4 cut(s) 263, 4422, 4580, 4668
AdeI CACNNNGTG 4 cut(s) 1550, 2741, 2999, 4097
AfiI CCNNNNNNNGG 9 cut(s) 45, 196, 387, 2968, 3889, 4097, 4388, 4552, 5027
AflII CTTAAG 1 cut(s) 4738
AflIII ACRYGT 2 cut(s) 1202, 2384
AhdI GACNNNNNGTC 2 cut(s) 625, 1855
AhlI ACTAGT 1 cut(s) 1886
AjnI CCWGG 9 cut(s) 792, 1147, 1894, 3796, 4381, 4498, 4535, 4606, 4706
AjuI GAANNNNNNNTTGG 2 cut(s) 2753, 2785
Alw21I GWGCWC 4 cut(s) 2192, 3004, 3190, 4666
Alw26I GTCTC 3 cut(s) 54, 221, 2648
AlwI GGATC 7 cut(s) 293, 1038, 1159, 1270, 2084, 4547, 5011
AlwNI CAGNNNCTG 1 cut(s) 1955
Ama87I CYCGRG 1 cut(s) 489
Aor13HI TCCGGA 1 cut(s) 2589
AoxI GGCC 6 cut(s) 169, 174, 1553, 1835, 3870, 4341
ApaI GGGCCC 1 cut(s) 3874
ArsI GACNNNNNNTTYG 2 cut(s) 4802, 4834
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 5 cut(s) 765, 889, 2043, 3135, 3675
Asp718I GGTACC 1 cut(s) 3032
AspA2I CCTAGG 1 cut(s) 3922
AspLEI GCGC 1 cut(s) 704
AspS9I GGNCC 6 cut(s) 170, 1553, 3243, 3606, 3870, 3871
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 6 cut(s) 826, 990, 1536, 3102, 4328, 4427
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 2 cut(s) 3243, 3606
AvrII CCTAGG 1 cut(s) 3922
BaeGI GKGCMC 2 cut(s) 3415, 3874
BanI GGYRCC 2 cut(s) 3032, 4532
BanII GRGCYC 3 cut(s) 3190, 3224, 3874
BbsI GAAGAC 5 cut(s) 732, 963, 1754, 2768, 4863
Bbv12I GWGCWC 4 cut(s) 2192, 3004, 3190, 4666
BceAI ACGGC 2 cut(s) 184, 226
BcgI CGANNNNNNTGC 4 cut(s) 2063, 2097, 2502, 2536
BciT130I CCWGG 9 cut(s) 794, 1149, 1896, 3798, 4383, 4500, 4537, 4608, 4708
BciVI GTATCC 1 cut(s) 3560
BclI TGATCA 2 cut(s) 1924, 1933
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 3 cut(s) 54, 221, 2648
BcuI ACTAGT 1 cut(s) 1886
BfmI CTRYAG 8 cut(s) 1383, 1391, 2496, 2953, 3486, 3534, 3656, 4139
BfoI RGCGCY 1 cut(s) 705
BfrI CTTAAG 1 cut(s) 4738
BfuAI ACCTGC 2 cut(s) 641, 911
BfuI GTATCC 1 cut(s) 3560
BglI GCCNNNNNGGC 1 cut(s) 3869
BglII AGATCT 1 cut(s) 848
BlnI CCTAGG 1 cut(s) 3922
BlpI GCTNAGC 1 cut(s) 2563
BmcAI AGTACT 3 cut(s) 2517, 2869, 2975
Bme18I GGWCC 2 cut(s) 3243, 3606
BmeRI GACNNNNNGTC 2 cut(s) 625, 1855
BmeT110I CYCGRG 1 cut(s) 489
BmgT120I GGNCC 6 cut(s) 170, 1553, 3243, 3606, 3870, 3871
BmiI GGNNCC 9 cut(s) 966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
BmrI ACTGGG 1 cut(s) 973
BmuI ACTGGG 1 cut(s) 973
BpiI GAAGAC 5 cut(s) 732, 963, 1754, 2768, 4863
BpmI CTGGAG 7 cut(s) 896, 1131, 1422, 1917, 4206, 4737, 4995
Bpu10I CCTNAGC 1 cut(s) 4929
Bpu1102I GCTNAGC 1 cut(s) 2563
BpuEI CTTGAG 3 cut(s) 851, 2256, 4058
BpuMI CCSGG 1 cut(s) 173
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 3 cut(s) 2345, 2387, 4094
BsaBI GATNNNNATC 5 cut(s) 1156, 3594, 3651, 4128, 4331
BsaI GGTCTC 1 cut(s) 54
BsaWI WCCGGW 2 cut(s) 403, 2589
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 4051, 4081, 4179, 4209
Bsc4I CCNNNNNNNGG 9 cut(s) 45, 196, 387, 2968, 3889, 4097, 4388, 4552, 5027
Bse3DI GCAATG 2 cut(s) 2220, 4966
Bse8I GATNNNNATC 5 cut(s) 1156, 3594, 3651, 4128, 4331
BseAI TCCGGA 1 cut(s) 2589
BseBI CCWGG 9 cut(s) 794, 1149, 1896, 3798, 4383, 4500, 4537, 4608, 4708
BseCI ATCGAT 1 cut(s) 180
BseJI GATNNNNATC 5 cut(s) 1156, 3594, 3651, 4128, 4331
BseLI CCNNNNNNNGG 9 cut(s) 45, 196, 387, 2968, 3889, 4097, 4388, 4552, 5027
BseMI GCAATG 2 cut(s) 2220, 4966
BseMII CTCAG 7 cut(s) 358, 689, 1068, 2188, 2488, 3149, 4943
BseRI GAGGAG 4 cut(s) 317, 507, 2994, 4059
BseSI GKGCMC 2 cut(s) 3415, 3874
BseYI CCCAGC 2 cut(s) 2438, 3873
BsgI GTGCAG 4 cut(s) 1455, 3606, 3924, 4817
Bsh1236I CGCG 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 4777
BshFI GGCC 6 cut(s) 171, 176, 1555, 1837, 3872, 4343
BshNI GGYRCC 2 cut(s) 3032, 4532
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 4777
BsiHKAI GWGCWC 4 cut(s) 2192, 3004, 3190, 4666
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 4 cut(s) 173, 254, 404, 2590
BslFI GGGAC 2 cut(s) 3445, 3504
BslI CCNNNNNNNGG 9 cut(s) 45, 196, 387, 2968, 3889, 4097, 4388, 4552, 5027
BsmAI GTCTC 3 cut(s) 54, 221, 2648
BsmFI GGGAC 2 cut(s) 3445, 3504
BsmI GAATGC 1 cut(s) 2994
BsnI GGCC 6 cut(s) 171, 176, 1555, 1837, 3872, 4343
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 489
Bsp120I GGGCCC 1 cut(s) 3870
Bsp1286I GDGCHC 7 cut(s) 2192, 3004, 3190, 3224, 3415, 3874, 4666
Bsp13I TCCGGA 1 cut(s) 2589
Bsp1407I TGTACA 4 cut(s) 749, 1525, 3983, 4429
Bsp1720I GCTNAGC 1 cut(s) 2563
Bsp19I CCATGG 2 cut(s) 1005, 4021
BspANI GGCC 6 cut(s) 171, 176, 1555, 1837, 3872, 4343
BspCNI CTCAG 7 cut(s) 357, 688, 1067, 2187, 2487, 3150, 4942
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 1 cut(s) 2589
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 1 cut(s) 4335
BspLI GGNNCC 9 cut(s) 966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
BspMAI CTGCAG 7 cut(s) 1387, 1395, 2957, 3490, 3538, 3660, 4143
BspMI ACCTGC 2 cut(s) 641, 911
BspPI GGATC 7 cut(s) 293, 1038, 1159, 1270, 2084, 4547, 5011
BspQI GCTCTTC 1 cut(s) 3144
BspT107I GGYRCC 2 cut(s) 3032, 4532
BspTI CTTAAG 1 cut(s) 4738
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 2 cut(s) 2220, 4966
BsrGI TGTACA 4 cut(s) 749, 1525, 3983, 4429
BssNAI GTATAC 1 cut(s) 2382
BssT1I CCWWGG 7 cut(s) 141, 441, 1005, 3250, 3636, 3922, 4021
Bst1107I GTATAC 1 cut(s) 2382
Bst2UI CCWGG 9 cut(s) 794, 1149, 1896, 3798, 4383, 4500, 4537, 4608, 4708
Bst6I CTCTTC 2 cut(s) 1546, 3144
BstAFI CTTAAG 1 cut(s) 4738
BstAPI GCANNNNNTGC 3 cut(s) 1241, 3860, 4118
BstAUI TGTACA 4 cut(s) 749, 1525, 3983, 4429
BstBAI YACGTR 3 cut(s) 2345, 2387, 4094
BstC8I GCNNGC 8 cut(s) 1495, 1557, 2015, 2857, 2887, 4043, 4666, 4781
BstDSI CCRYGG 2 cut(s) 1005, 4021
BstEII GGTNACC 2 cut(s) 796, 4507
BstFNI CGCG 1 cut(s) 241
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 1 cut(s) 704
BstMAI GTCTC 3 cut(s) 54, 221, 2648
BstMCI CGRYCG 1 cut(s) 4777
BstNI CCWGG 9 cut(s) 794, 1149, 1896, 3798, 4383, 4500, 4537, 4608, 4708
BstNSI RCATGY 6 cut(s) 913, 1206, 1691, 2759, 2889, 5036
BstPI GGTNACC 2 cut(s) 796, 4507
BstSFI CTRYAG 8 cut(s) 1383, 1391, 2496, 2953, 3486, 3534, 3656, 4139
BstSLI GKGCMC 2 cut(s) 3415, 3874
BstUI CGCG 1 cut(s) 241
BstV2I GAAGAC 5 cut(s) 732, 963, 1754, 2768, 4863
BstX2I RGATCY 3 cut(s) 848, 1262, 5016
BstXI CCANNNNNNTGG 1 cut(s) 3371
BstYI RGATCY 3 cut(s) 848, 1262, 5016
BstZ17I GTATAC 1 cut(s) 2382
Bsu15I ATCGAT 1 cut(s) 180
BsuI GTATCC 1 cut(s) 3560
BsuRI GGCC 6 cut(s) 171, 176, 1555, 1837, 3872, 4343
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 2 cut(s) 1005, 4021
BtsI GCAGTG 1 cut(s) 4867
BveI ACCTGC 2 cut(s) 641, 911
Cac8I GCNNGC 8 cut(s) 1495, 1557, 2015, 2857, 2887, 4043, 4666, 4781
CaiI CAGNNNCTG 1 cut(s) 1955
CciI TCATGA 1 cut(s) 4335
CfoI GCGC 1 cut(s) 704
Cfr13I GGNCC 6 cut(s) 170, 1553, 3243, 3606, 3870, 3871
ClaI ATCGAT 1 cut(s) 180
CsiI ACCWGGT 2 cut(s) 792, 4706
CspCI CAANNNNNGTGG 6 cut(s) 1344, 1379, 2608, 2643, 3697, 3732
DraI TTTAAA 1 cut(s) 3385
DraIII CACNNNGTG 4 cut(s) 1550, 2741, 2999, 4097
DriI GACNNNNNGTC 2 cut(s) 625, 1855
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 2 cut(s) 1546, 3144
Eam1105I GACNNNNNGTC 2 cut(s) 625, 1855
EarI CTCTTC 2 cut(s) 1546, 3144
EciI GGCGGA 2 cut(s) 113, 1731
Ecl136II GAGCTC 1 cut(s) 3188
Eco130I CCWWGG 7 cut(s) 141, 441, 1005, 3250, 3636, 3922, 4021
Eco147I AGGCCT 1 cut(s) 4343
Eco24I GRGCYC 3 cut(s) 3190, 3224, 3874
Eco31I GGTCTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 2 cut(s) 3243, 3606
Eco53kI GAGCTC 1 cut(s) 3188
Eco57I CTGAAG 4 cut(s) 263, 4422, 4580, 4668
Eco88I CYCGRG 1 cut(s) 489
Eco91I GGTNACC 2 cut(s) 796, 4507
EcoICRI GAGCTC 1 cut(s) 3188
EcoO65I GGTNACC 2 cut(s) 796, 4507
EcoRI GAATTC 3 cut(s) 339, 561, 3970
EcoRII CCWGG 9 cut(s) 792, 1147, 1894, 3796, 4381, 4498, 4535, 4606, 4706
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 7 cut(s) 141, 441, 1005, 3250, 3636, 3922, 4021
EcoT22I ATGCAT 1 cut(s) 2751
EcoT38I GRGCYC 3 cut(s) 3190, 3224, 3874
ErhI CCWWGG 7 cut(s) 141, 441, 1005, 3250, 3636, 3922, 4021
FalI AAGNNNNNCTT 4 cut(s) 753, 785, 3120, 3152
FaqI GGGAC 2 cut(s) 3445, 3504
FauI CCCGC 1 cut(s) 122
FauNDI CATATG 2 cut(s) 3174, 4681
FbaI TGATCA 2 cut(s) 1924, 1933
FblI GTMKAC 1 cut(s) 2381
FriOI GRGCYC 3 cut(s) 3190, 3224, 3874
GlaI GCGC 1 cut(s) 703
GsaI CCCAGC 2 cut(s) 2442, 3877
GsuI CTGGAG 7 cut(s) 896, 1131, 1422, 1917, 4206, 4737, 4995
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 6 cut(s) 171, 176, 1555, 1837, 3872, 4343
HapII CCGG 4 cut(s) 173, 254, 404, 2590
HhaI GCGC 1 cut(s) 704
Hin6I GCGC 1 cut(s) 702
HinP1I GCGC 1 cut(s) 702
HincII GTYRAC 2 cut(s) 1903, 2068
HindII GTYRAC 2 cut(s) 1903, 2068
HindIII AAGCTT 4 cut(s) 869, 1877, 3088, 4562
HpaII CCGG 4 cut(s) 173, 254, 404, 2590
HphI GGTGA 6 cut(s) 826, 990, 1536, 3102, 4328, 4427
Hpy166II GTNNAC 9 cut(s) 901, 1429, 1903, 2068, 2119, 2382, 2575, 4182, 5030
Hpy8I GTNNAC 9 cut(s) 901, 1429, 1903, 2068, 2119, 2382, 2575, 4182, 5030
Hpy99I CGWCG 3 cut(s) 170, 209, 212
HpyCH4IV ACGT 5 cut(s) 1130, 2344, 2386, 2585, 4093
HpySE526I ACGT 5 cut(s) 1130, 2344, 2386, 2585, 4093
HspAI GCGC 1 cut(s) 702
Kpn2I TCCGGA 1 cut(s) 2589
KpnI GGTACC 1 cut(s) 3036
Ksp22I TGATCA 2 cut(s) 1924, 1933
LguI GCTCTTC 1 cut(s) 3144
MabI ACCWGGT 2 cut(s) 792, 4706
MaeII ACGT 5 cut(s) 1130, 2344, 2386, 2585, 4093
MaeIII GTNAC 9 cut(s) 193, 684, 796, 2084, 2104, 2123, 2469, 4507, 5086
MfeI CAATTG 1 cut(s) 2568
MflI RGATCY 3 cut(s) 848, 1262, 5016
MhlI GDGCHC 7 cut(s) 2192, 3004, 3190, 3224, 3415, 3874, 4666
MmeI TCCRAC 9 cut(s) 63, 710, 1008, 2751, 3270, 3352, 3544, 4498, 4876
Mph1103I ATGCAT 1 cut(s) 2751
MroI TCCGGA 1 cut(s) 2589
MroXI GAANNNNTTC 5 cut(s) 765, 889, 2043, 3135, 3675
MslI CAYNNNNRTG 9 cut(s) 693, 908, 1692, 1979, 2628, 2754, 2884, 3173, 4791
MspA1I CMGCKG 5 cut(s) 1373, 2306, 2483, 4041, 4843
MspCI CTTAAG 1 cut(s) 4738
MspI CCGG 4 cut(s) 173, 254, 404, 2590
MunI CAATTG 1 cut(s) 2568
Mva1269I GAATGC 1 cut(s) 2994
MvaI CCWGG 9 cut(s) 794, 1149, 1896, 3798, 4383, 4500, 4537, 4608, 4708
MvnI CGCG 1 cut(s) 241
NciI CCSGG 1 cut(s) 173
NcoI CCATGG 2 cut(s) 1005, 4021
NdeI CATATG 2 cut(s) 3174, 4681
NlaIV GGNNCC 9 cut(s) 966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 3 cut(s) 193, 2084, 5086
NsiI ATGCAT 1 cut(s) 2751
NspI RCATGY 6 cut(s) 913, 1206, 1691, 2759, 2889, 5036
PaeI GCATGC 1 cut(s) 2889
PaeR7I CTCGAG 1 cut(s) 489
PagI TCATGA 1 cut(s) 4335
PaqCI CACCTGC 1 cut(s) 911
PceI AGGCCT 1 cut(s) 4343
PciI ACATGT 1 cut(s) 1202
PciSI GCTCTTC 1 cut(s) 3144
PcsI WCGNNNNNNNCGW 2 cut(s) 153, 4090
PctI GAATGC 1 cut(s) 2994
PdmI GAANNNNTTC 5 cut(s) 765, 889, 2043, 3135, 3675
PflMI CCANNNNNTGG 4 cut(s) 2968, 3889, 4097, 5027
PfoI TCCNGGA 2 cut(s) 1147, 3796
Ppu21I YACGTR 3 cut(s) 2345, 2387, 4094
PscI ACATGT 1 cut(s) 1202
PshBI ATTAAT 1 cut(s) 612
PsiI TTATAA 2 cut(s) 2409, 3773
Psp124BI GAGCTC 1 cut(s) 3190
Psp6I CCWGG 9 cut(s) 792, 1147, 1894, 3796, 4381, 4498, 4535, 4606, 4706
PspEI GGTNACC 2 cut(s) 796, 4507
PspFI CCCAGC 2 cut(s) 2438, 3873
PspGI CCWGG 9 cut(s) 792, 1147, 1894, 3796, 4381, 4498, 4535, 4606, 4706
PspN4I GGNNCC 9 cut(s) 966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
PspOMI GGGCCC 1 cut(s) 3870
PspPI GGNCC 6 cut(s) 170, 1553, 3243, 3606, 3870, 3871
PspXI VCTCGAGB 1 cut(s) 489
PstI CTGCAG 7 cut(s) 1387, 1395, 2957, 3490, 3538, 3660, 4143
PstNI CAGNNNCTG 1 cut(s) 1955
PsuI RGATCY 3 cut(s) 848, 1262, 5016
PvuII CAGCTG 4 cut(s) 1373, 2306, 2483, 4843
RseI CAYNNNNRTG 9 cut(s) 693, 908, 1692, 1979, 2628, 2754, 2884, 3173, 4791
SacI GAGCTC 1 cut(s) 3190
SapI GCTCTTC 1 cut(s) 3144
Sau96I GGNCC 6 cut(s) 170, 1553, 3243, 3606, 3870, 3871
ScaI AGTACT 3 cut(s) 2517, 2869, 2975
SduI GDGCHC 7 cut(s) 2192, 3004, 3190, 3224, 3415, 3874, 4666
SexAI ACCWGGT 2 cut(s) 792, 4706
SfcI CTRYAG 8 cut(s) 1383, 1391, 2496, 2953, 3486, 3534, 3656, 4139
Sfr274I CTCGAG 1 cut(s) 489
SinI GGWCC 2 cut(s) 3243, 3606
SlaI CTCGAG 1 cut(s) 489
SmiI ATTTAAAT 1 cut(s) 3385
SmiMI CAYNNNNRTG 9 cut(s) 693, 908, 1692, 1979, 2628, 2754, 2884, 3173, 4791
SmlI CTYRAG 5 cut(s) 489, 866, 2271, 4073, 4738
SmoI CTYRAG 5 cut(s) 489, 866, 2271, 4073, 4738
SpeI ACTAGT 1 cut(s) 1886
SphI GCATGC 1 cut(s) 2889
SseBI AGGCCT 1 cut(s) 4343
SspI AATATT 4 cut(s) 2637, 2690, 2934, 3754
SstI GAGCTC 1 cut(s) 3190
StuI AGGCCT 1 cut(s) 4343
StyI CCWWGG 7 cut(s) 141, 441, 1005, 3250, 3636, 3922, 4021
SwaI ATTTAAAT 1 cut(s) 3385
TaiI ACGT 5 cut(s) 1133, 2347, 2389, 2588, 4096
TatI WGTACW 9 cut(s) 749, 1525, 1681, 2477, 2515, 2867, 2973, 3983, 4429
TauI GCSGC 6 cut(s) 76, 79, 82, 85, 88, 367
TseFI GTSAC 3 cut(s) 193, 2084, 5086
Tsp45I GTSAC 3 cut(s) 193, 2084, 5086
TspGWI ACGGA 4 cut(s) 212, 957, 1644, 2708
Van91I CCANNNNNTGG 4 cut(s) 2968, 3889, 4097, 5027
Vha464I CTTAAG 1 cut(s) 4738
VpaK11BI GGWCC 2 cut(s) 3243, 3606
VspI ATTAAT 1 cut(s) 612
XbaI TCTAGA 3 cut(s) 3139, 4658, 4699
XceI RCATGY 6 cut(s) 913, 1206, 1691, 2759, 2889, 5036
XcmI CCANNNNNNNNNTGG 1 cut(s) 2555
XhoI CTCGAG 1 cut(s) 489
XmaJI CCTAGG 1 cut(s) 3922
XmiI GTMKAC 1 cut(s) 2381
XmnI GAANNNNTTC 5 cut(s) 765, 889, 2043, 3135, 3675
ZrmI AGTACT 3 cut(s) 2517, 2869, 2975
Zsp2I ATGCAT 1 cut(s) 2751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.