Rroxscaffold_5G00378000

Protein PHYLLO, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006265
Physical Location & Seq
Forward (+)
58520149 .. 58540681
20533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00378000.1

Sequence Viewer

Length: 5295 bp
ATGGGAGGCCCTCCCAATGTATGGTACAGAGATGGTGGTGCTGGCCGGAGTGGTCATGGAAACAAGGTGCAAAGGGCACCTAAGAACCCTTCGGTCAAACGGGATAGAGTTGGCAATCAACCATGCTATAGGTGTGGAGTTATTGGGCATTGGTACAAGAATTGCCAAGCAAGCAACATAGTTGCAGCCACTTACAAGAGGTATAGGGAGTCTAAAGAACAAGAAGCTCACTATATGGAAGAAGAAGGTCATGACCCGGATGTCAACCTCACTATTGCGATACAAGGAGATATCTGTGGACCTATCCATCCGAATGCGGACCATTCAAGTACTTTATGGTTCGGTGGATGCTTTGACATGCTGGTCACATGTCGCACCGTAACTACACTTAGTGGAGGCCTTGAACTAGAACACCGAATCATTGCTCTACATGAGGCGGTTTGTGAGTATCATACGCCGAAAAGAGAACAATCTTCCACCTATGTTATCGATAGACGAACGACCATGGTACCGGTAGGAGTCCGCATCATAAAGTCTCTCCCTCGCACCAAACCCTCGCTGCCGCCGCCTTTCCCGTCGTTCGAGGCGATCAAGGAGGCGGTTGAGGAGTTGAAGCTCAGTCCTCCTTCAACTTGTAGTGGAATTCTCAGGTTTCAGGTGGCTGTGCCGCCGAGTGCGAAAGCCTTGAACTGTTTTCGAATCGGTGCTGCAATTTACTTCACACCTTCTTCTAATTCTTCTTCTTCGATCAAAAGAATTCTATCAAATGAATCAGCTTTTGTAATAGCTTATGGATTTATGGATATTAATTACGACCAAGAGTCATCTTATATGAAGCATGAGGCAGGTTCATATAACTTTTTTATTCCTCAGATTGAGTTACATGAGCATGAGGGCGCTTCTATATTAGCAGCAACTATTGGTTGGAGTGAGTCTTCCCTTTGTACATTTGAGGAAGCTGTTCTTTCTTATGAGTTATGTTTCAACCAGGTTACCTGTCAAGTTTGTTCCACAACAACGAAATCCCACACCACAAATATAAGATCTACTCTTAGGAAGCTCAATCTTCTGGAGGATGGAATGGTTTCTATGGTACACATGAGCATGCTGTTAGCAGGTGGAAAAAACATTTCGTCTGACATCATGGCACTGAAAGAGGCTCCGTCTTCCTCCCAGTTTTGTGTGAGGCTTTCACCTACCATGGCTGTTGCTAATAAAATGTTGGATCATGCCAGTAAAATGTGCTTCTCAGTGCAAGATTGTGGCAACATCAACACTGTTTGGGCATCACTTATAGTTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCCCTTGCTGTTGCTGCATCCACTCATCCACTTATTACATGTATTGTATGTTATGATGAACGCTCACTTGCATTTCATGCTGTTGGGTATGCAAGAGGATCTGGCAAACCAGCAGTAGTCATAACATCATCTGGCACTGCAGTTTCAAATCTTCTTCCTGCTGTGGTCGAGGCTAGTCAAGATTTTGTACCACTTATATTGCTCACAGCCGATCGTCCTGCAGAACTGCAGGATGCTGGAGCAAACCAAGCAATCAATCAGGTAAACCATTTTGGCTCTTTTGCACGGTTTTTCTTTAGCCTTCCTGCACCCACAGATCATATTTCAGCTCGCATGGTGCTTACAACACTTGACAATGCTGTACATTGGGCAACCTCTTCACCATGTGGTCCTGTTCATATTAACTGTCCTTTCAGGGAGCCACTAGAAGATAGCCCTAGAAAATGGATGTTGAGTTGTTTGAATGGATTAAACCATTGGATGTCTAATTCTGAACCATTTACCAAGTATATTCAAGTACAACATGCTCATACAAGTAATGATGGTTGTGGCGGAATGTCAGAAATTCTAAATGTCGTTAAGGGAACTAACAAGGGTCTTCTACTTATCGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTCTTGGCTAAACACCTTCACTGGCCTGTTATAGCTGACATCTTGTCAGGATTACGATTGAAAAAGCTTTTGACTAGTTTCCCTGAAGTTGACAATGATTTATTGTTTGTTGATCATCTTGACCATGCCCTACTGTCAGATTCTGTCAGTAAATGGATTAACATTGATGTGATTATTCAGATTGGGAGCAGGATTACAAGCAAGCGTATTGCTAAGATGCTAGAGGAATCCTTTCCCTGCTCATATATCATGGTTGACAAGCATCCATTTCGTCACGATCCTTCTCATATTGTAACTCATAGAGTCCACAGTAACATTTTTGAATTTGCTGATTATATCCTTAAAGCCGAATTTCCTCATCTCAGCAAGGAATGGAGCACTTATCTTCAAATGATAAATGCAATGGTTGAAAGGGAGTTGTCATTTCAAATTTCTGCTAGACACTCCTTGACAGAACCTCAAGTCGCATACCTAATTTCAGAAGCACTTTCAGCTGAATCTGCTCTTTTCATTGGGAACAGTATGGCAATACGTGATGCAGATATGTATGGGCGTGGTTGGTCAGAGTATACACGTAGTATTGTTGCCATGAATTATAAACTACCCTGTCAAATGATTCGGGTGGCTGGGAATAGAGGTGCAAGCGGTATTGATGGGTTACTCAGTACAGCTGTTGGCTTTGCTATAGGATGCAATAAACGAGTACTTTGTGTGATTGGAGATGTTTCCTTCCTCCATGATACAAATGGCTTAGCAATTGTGACCCAGAGGACGTTCCGGAAACCAATGACGATAGTTGTGATTAACAATCATGGTGGTGCAATATTCAGTCTCCTTCCTCTTGCAGATAGAGTCAAACCAAGAATACTAAACCAATATTTCTACACCTCTCACAACGTATCAATCCGTGAGCTTTGTGTAGCACATGGTGTGATGCATCTACATGCGACGACAAAGTTGGATTTTGAAGAAGCGTTATTCACATCTCAACATGGAGGGATGGATTGTGTCATTGAAGTTGAGAGCTGCATAGATACCAATGCCAGCTTTCACAGTACTTTAAAAACATTTGCATGCCAAGCAGCAGATCAAGTCATAAAGCCTTCTAGTCAAGACTCGATATTAGATGGCAGTTTACTCTGCAGAGTCCATAGAATGGAGTACTCACTGTTTAGAATGCCACTGTGTGCTCCTCATACTATGGTTTCTGTTGATGGCGGTACCACCAGCTTCCACAGGGAAGGGTTTATTTTAACTTTGTATTTTGAAGATGGAAGCTTTGGGCTTGGCGAGGTTTCACCCGTAGACATCTACAAAGAAAACCTTGTAGATGTAGAAGAGCAACTGAGGTTTCTCATCCATATGATGAAAGGAGCTCATATCAGTTGCTTCCTTCCTCTGCTAAAGGGCTCGTTTTCATCTTGGATATGGTCCAACCTTGGAATCCTGCCATGTACGCTCTTTCCTAGTGTGAGATGTGGTTTGGAAATGGCAATTCTCAATGCCATAGCAAATAGAAAAGGTTCCAATTTATTAGGAATACTCCATCCCCAGATAGTTGGAGATAAATTTAAATCGTCGTCAACGGTTCAGATTTGTGCCCTTGTTGATTCCAGTGGGACACCCACACAAGTAGCTGATGCTATTGCTACACTTGTTGAAGAAGGATTTACTGCAGTAAAGATTAAGGTCCTACTTTCTTGTAATCCAAAACATCATTCTTACAGAGCATATGTAAATGTGGGTAATCTATACATCAAATTAAACAAATGCTTTGATGCCAATCGGAACTGGACCTATGAAGAAGCTATCCAGTTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATCGAGGAACCTGTTCAAATTGAAGAAGATATTATCAAGTTTTGCGAAGAAAGTGGGTTGCCTGTGGCACTGGATGAAACTATTGACAATATTGGAGAACATCCACTTATAAAGCTCTCAAAGTATGCCCATCCAGGAATAGTAGCTGTTGTCATCAAGCCAAGTGTTGTTGGTGGCTTTGAACATGCAGCAATAATTGCCCAATGGGCCCAGCAGCACCAGAAAATGGCTGTTGTTAGTGCTGCATTTGAAAGCAGCCTAGGTTTGTCAGCATATATTCAGTTTTCCTGTTATCTCAACCAGATGAATTCAGAGATTTGTACAATGATGAACTATCCATTAGCCTCATCCATAGCCCATGGTTTAGGAACTTACCGCTGGCTAAAAGAAGATTTGACGACTACTCCTCTCAAGATAAATCGTAATCCACGTAGTGGGTTCGTAGAAGCATCTGTTGCTGATTCTGATCGAGTTCTGCAGCAATTTCAAATCAATGGAAACATAATTCGTAGAAAGTTTACTGGAGAGCAAGTTTGTATATACCAGTTGCCTTTAGATTCAAAGGGTTTATCTTGCTCCATCAAAATTCAGGAGATTGGACAAGGATATGATGATAATGTATGTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATCATGAAGGCCTTATCAGGATGTGGAAGATGCGTTTCTATTGACCTTCCTGGTCATGGTGGAACCAAGATACAAAGTCATGGTGATGAGGATGTTGTACAGGCTTCAGGTTTGTGTGTTGAGGTAGTTGCCGATCTTTTATGTAAAGTGATTGAGCATATTACTCCTGGGAAGGTTACCCTTGTCGGATATTCTATGGGAGCCAGGATCGCCCTTTGCATGGCTCTGAAGCTTACTAAAAAAGTTAAGGGAGCTATTATCATATCTGGCAGCCCTGGATTGAATGATGGAATGGCAAGAAAAATTCGTAGAGCTGAAGATGACTCTAGAGCACGCTTCCTTGCTGCATATGGCTTAAAGCACTTTCTAGACACCTGGTATGCTGGAAAGCTTTGGAACAGCTTAAGGGAACACCCTCACTTTCATCAGATAGCCGCCGACCGCCTGAATCGTGCAGATGTGCAGAGTCTTGCAAAAGTTCTGTCTGCTTTAAGTGTTGGGAGGCAGCTGCCATTGTGGGAAGACTTGAAGCACTGCAAAACGCCTCTTTTGCTCATTGTTGGAGAGAAAGATAAAAAATTCAAAACAATAGCTCAGGATATGTTCCTTGCGATTGGTAGTGGCAATGGCAAATTGACTGGAGATGATGGCGCACCCAACGACCTTTGTGAGATTGTTGAGATCCCAGATTGTGGACATGCTGCTCATCTGGAAAATCCTCTCCCTGTAATCAGGGCATTAAGGCAATTTGTGATGAAACTGAACTGA

Protein Analysis

1764

Amino Acids

194.32

Weight (kDa)

6.51

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 429 - 541 3.8e-22 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2 PF16582 607 - 840 2e-23 Middle domain of thiamine pyrophosphate
TPP_enzyme_C PF02775 890 - 999 7.1e-07 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C PF13378 1214 - 1389 1.8e-14 Enolase C-terminal domain-like
Hydrolase_4 PF12146 1492 - 1711 1.4e-11 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 1495 - 1754 1.3e-22 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 1495 - 1749 6.3e-19 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2604, 3968
AarI CACCTGC 1 cut(s) 1106
AasI GACNNNNNNGTC 2 cut(s) 260, 362
Acc36I ACCTGC 2 cut(s) 836, 1106
Acc65I GGTACC 2 cut(s) 508, 3227
AccB1I GGYRCC 3 cut(s) 76, 508, 3227
AccB7I CCANNNNNTGG 5 cut(s) 21, 3163, 4084, 4292, 5219
AccI GTMKAC 2 cut(s) 2576, 3312
AccIII TCCGGA 1 cut(s) 2784
AclWI GGATC 6 cut(s) 1233, 1354, 1465, 2279, 4742, 5203
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 4 cut(s) 2112, 4617, 4775, 4863
AdeI CACNNNGTG 5 cut(s) 392, 1745, 2936, 3194, 4292
AfiI CCNNNNNNNGG 7 cut(s) 21, 3163, 4084, 4292, 4583, 4747, 5219
AflII CTTAAG 1 cut(s) 4930
AflIII ACRYGT 3 cut(s) 368, 1397, 2579
AgeI ACCGGT 1 cut(s) 511
AhdI GACNNNNNGTC 2 cut(s) 820, 2050
AhlI ACTAGT 1 cut(s) 2081
AjnI CCWGG 8 cut(s) 987, 1342, 3991, 4576, 4693, 4730, 4801, 4901
AjuI GAANNNNNNNTTGG 2 cut(s) 3709, 3741
Alw21I GWGCWC 4 cut(s) 2387, 3199, 3385, 4861
Alw26I GTCTC 2 cut(s) 540, 2843
AlwI GGATC 6 cut(s) 1233, 1354, 1465, 2279, 4742, 5203
AlwNI CAGNNNCTG 1 cut(s) 2150
Aor13HI TCCGGA 1 cut(s) 2784
AoxI GGCC 6 cut(s) 7, 43, 397, 2030, 4065, 4536
ApaI GGGCCC 1 cut(s) 4069
ArsI GACNNNNNNTTYG 2 cut(s) 4994, 5026
AseI ATTAAT 1 cut(s) 807
AsiGI ACCGGT 1 cut(s) 511
Asp700I GAANNNNTTC 5 cut(s) 960, 1084, 2238, 3529, 3870
Asp718I GGTACC 2 cut(s) 508, 3227
AspA2I CCTAGG 1 cut(s) 4117
AspLEI GCGC 2 cut(s) 899, 5180
AspS9I GGNCC 9 cut(s) 8, 299, 319, 1748, 3438, 3697, 3801, 4065, 4066
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 5 cut(s) 1185, 1731, 3297, 4523, 4622
AsuII TTCGAA 1 cut(s) 697
AvaII GGWCC 6 cut(s) 299, 319, 1748, 3438, 3697, 3801
AvrII CCTAGG 1 cut(s) 4117
BaeGI GKGCMC 3 cut(s) 79, 3610, 4069
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BanI GGYRCC 3 cut(s) 76, 508, 3227
BanII GRGCYC 3 cut(s) 3385, 3419, 4069
BbsI GAAGAC 4 cut(s) 927, 1158, 1949, 5055
Bbv12I GWGCWC 4 cut(s) 2387, 3199, 3385, 4861
BcgI CGANNNNNNTGC 4 cut(s) 1559, 1593, 2258, 2292
BciT130I CCWGG 8 cut(s) 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
BclI TGATCA 1 cut(s) 2119
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 2 cut(s) 540, 2843
BcuI ACTAGT 1 cut(s) 2081
BfmI CTRYAG 9 cut(s) 127, 1497, 1578, 1586, 2691, 3148, 3681, 3851, 4334
BfoI RGCGCY 1 cut(s) 900
BfrI CTTAAG 1 cut(s) 4930
BfuAI ACCTGC 2 cut(s) 836, 1106
BglI GCCNNNNNGGC 1 cut(s) 4064
BglII AGATCT 1 cut(s) 1043
BlnI CCTAGG 1 cut(s) 4117
BlpI GCTNAGC 1 cut(s) 2758
BmcAI AGTACT 4 cut(s) 331, 2712, 3064, 3170
Bme1390I CCNGG 9 cut(s) 257, 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
Bme18I GGWCC 6 cut(s) 299, 319, 1748, 3438, 3697, 3801
BmeRI GACNNNNNGTC 2 cut(s) 820, 2050
BmgT120I GGNCC 9 cut(s) 8, 299, 319, 1748, 3438, 3697, 3801, 4065, 4066
BmrFI CCNGG 9 cut(s) 257, 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
BmrI ACTGGG 1 cut(s) 1168
BmuI ACTGGG 1 cut(s) 1168
BpiI GAAGAC 4 cut(s) 927, 1158, 1949, 5055
BpmI CTGGAG 5 cut(s) 1091, 1326, 1617, 4401, 5187
Bpu10I CCTNAGC 1 cut(s) 5121
Bpu1102I GCTNAGC 1 cut(s) 2758
Bpu14I TTCGAA 1 cut(s) 697
BpuEI CTTGAG 2 cut(s) 2451, 4253
BpuMI CCSGG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 489
BsaAI YACGTR 3 cut(s) 2540, 2582, 4289
BsaBI GATNNNNATC 5 cut(s) 1351, 3789, 3846, 4323, 4526
BsaJI CCNNGG 8 cut(s) 504, 1200, 3445, 3831, 4117, 4216, 4694, 4801
BsaWI WCCGGW 2 cut(s) 511, 2784
BsaXI ACNNNNNCTCC 4 cut(s) 4246, 4276, 4374, 4404
Bsc4I CCNNNNNNNGG 7 cut(s) 21, 3163, 4084, 4292, 4583, 4747, 5219
Bse118I RCCGGY 1 cut(s) 511
Bse3DI GCAATG 3 cut(s) 420, 2415, 5158
Bse8I GATNNNNATC 5 cut(s) 1351, 3789, 3846, 4323, 4526
BseAI TCCGGA 1 cut(s) 2784
BseBI CCWGG 8 cut(s) 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
BseCI ATCGAT 1 cut(s) 489
BseDI CCNNGG 8 cut(s) 504, 1200, 3445, 3831, 4117, 4216, 4694, 4801
BseJI GATNNNNATC 5 cut(s) 1351, 3789, 3846, 4323, 4526
BseLI CCNNNNNNNGG 7 cut(s) 21, 3163, 4084, 4292, 4583, 4747, 5219
BseMI GCAATG 3 cut(s) 420, 2415, 5158
BseMII CTCAG 8 cut(s) 631, 661, 884, 1263, 2383, 2683, 3344, 5135
BseRI GAGGAG 3 cut(s) 620, 3189, 4254
BseSI GKGCMC 3 cut(s) 79, 3610, 4069
BseYI CCCAGC 2 cut(s) 2633, 4068
BsgI GTGCAG 3 cut(s) 1650, 5001, 5009
Bsh1285I CGRYCG 2 cut(s) 1573, 4969
BshFI GGCC 6 cut(s) 9, 45, 399, 2032, 4067, 4538
BshNI GGYRCC 3 cut(s) 76, 508, 3227
BshTI ACCGGT 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 489
BsiEI CGRYCG 2 cut(s) 1573, 4969
BsiHKAI GWGCWC 4 cut(s) 2387, 3199, 3385, 4861
BsiSI CCGG 4 cut(s) 46, 257, 512, 2785
BslFI GGGAC 1 cut(s) 3640
BslI CCNNNNNNNGG 7 cut(s) 21, 3163, 4084, 4292, 4583, 4747, 5219
BsmAI GTCTC 2 cut(s) 540, 2843
BsmFI GGGAC 1 cut(s) 3640
BsmI GAATGC 2 cut(s) 319, 3189
BsnI GGCC 6 cut(s) 9, 45, 399, 2032, 4067, 4538
Bsp119I TTCGAA 1 cut(s) 697
Bsp120I GGGCCC 1 cut(s) 4065
Bsp1286I GDGCHC 8 cut(s) 79, 2387, 3199, 3385, 3419, 3610, 4069, 4861
Bsp13I TCCGGA 1 cut(s) 2784
Bsp1407I TGTACA 4 cut(s) 944, 1720, 4178, 4624
Bsp1720I GCTNAGC 1 cut(s) 2758
Bsp19I CCATGG 3 cut(s) 504, 1200, 4216
BspANI GGCC 6 cut(s) 9, 45, 399, 2032, 4067, 4538
BspCNI CTCAG 8 cut(s) 630, 660, 883, 1262, 2382, 2682, 3345, 5134
BspDI ATCGAT 1 cut(s) 489
BspEI TCCGGA 1 cut(s) 2784
BspHI TCATGA 2 cut(s) 250, 4530
BspMAI CTGCAG 7 cut(s) 1501, 1582, 1590, 3152, 3685, 3855, 4338
BspMI ACCTGC 2 cut(s) 836, 1106
BspPI GGATC 6 cut(s) 1233, 1354, 1465, 2279, 4742, 5203
BspQI GCTCTTC 1 cut(s) 3339
BspT104I TTCGAA 1 cut(s) 697
BspT107I GGYRCC 3 cut(s) 76, 508, 3227
BspTI CTTAAG 1 cut(s) 4930
BsrDI GCAATG 3 cut(s) 420, 2415, 5158
BsrFI RCCGGY 1 cut(s) 511
BsrGI TGTACA 4 cut(s) 944, 1720, 4178, 4624
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 8 cut(s) 504, 1200, 3445, 3831, 4117, 4216, 4694, 4801
BssNAI GTATAC 1 cut(s) 2577
BssT1I CCWWGG 6 cut(s) 504, 1200, 3445, 3831, 4117, 4216
Bst1107I GTATAC 1 cut(s) 2577
Bst2UI CCWGG 8 cut(s) 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
Bst6I CTCTTC 2 cut(s) 1741, 3339
BstAFI CTTAAG 1 cut(s) 4930
BstAPI GCANNNNNTGC 3 cut(s) 1436, 4055, 4313
BstAUI TGTACA 4 cut(s) 944, 1720, 4178, 4624
BstBAI YACGTR 3 cut(s) 2540, 2582, 4289
BstBI TTCGAA 1 cut(s) 697
BstDSI CCRYGG 3 cut(s) 504, 1200, 4216
BstEII GGTNACC 2 cut(s) 991, 4702
BstH2I RGCGCY 1 cut(s) 900
BstHHI GCGC 2 cut(s) 899, 5180
BstMAI GTCTC 2 cut(s) 540, 2843
BstMCI CGRYCG 2 cut(s) 1573, 4969
BstNI CCWGG 8 cut(s) 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
BstNSI RCATGY 9 cut(s) 361, 372, 1108, 1401, 1886, 2954, 3084, 4046, 5228
BstPI GGTNACC 2 cut(s) 991, 4702
BstSCI CCNGG 9 cut(s) 255, 987, 1342, 3991, 4576, 4693, 4730, 4801, 4901
BstSFI CTRYAG 9 cut(s) 127, 1497, 1578, 1586, 2691, 3148, 3681, 3851, 4334
BstSLI GKGCMC 3 cut(s) 79, 3610, 4069
BstV2I GAAGAC 4 cut(s) 927, 1158, 1949, 5055
BstX2I RGATCY 3 cut(s) 1043, 1457, 5208
BstXI CCANNNNNNTGG 1 cut(s) 3566
BstYI RGATCY 3 cut(s) 1043, 1457, 5208
BstZ17I GTATAC 1 cut(s) 2577
Bsu15I ATCGAT 1 cut(s) 489
BsuRI GGCC 6 cut(s) 9, 45, 399, 2032, 4067, 4538
BsuTUI ATCGAT 1 cut(s) 489
BtgI CCRYGG 3 cut(s) 504, 1200, 4216
BtsI GCAGTG 2 cut(s) 1494, 5059
BveI ACCTGC 2 cut(s) 836, 1106
CaiI CAGNNNCTG 1 cut(s) 2150
CciI TCATGA 2 cut(s) 250, 4530
CfoI GCGC 2 cut(s) 899, 5180
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 9 cut(s) 8, 299, 319, 1748, 3438, 3697, 3801, 4065, 4066
ClaI ATCGAT 1 cut(s) 489
CsiI ACCWGGT 2 cut(s) 987, 4901
CspAI ACCGGT 1 cut(s) 511
CspCI CAANNNNNGTGG 4 cut(s) 1539, 1574, 2803, 2838
DraI TTTAAA 2 cut(s) 3069, 3580
DraIII CACNNNGTG 5 cut(s) 392, 1745, 2936, 3194, 4292
DrdI GACNNNNNNGTC 2 cut(s) 260, 362
DriI GACNNNNNGTC 2 cut(s) 820, 2050
DseDI GACNNNNNNGTC 2 cut(s) 260, 362
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 2 cut(s) 1741, 3339
Eam1105I GACNNNNNGTC 2 cut(s) 820, 2050
EarI CTCTTC 2 cut(s) 1741, 3339
EciI GGCGGA 1 cut(s) 1926
Ecl136II GAGCTC 1 cut(s) 3383
Eco130I CCWWGG 6 cut(s) 504, 1200, 3445, 3831, 4117, 4216
Eco147I AGGCCT 2 cut(s) 399, 4538
Eco24I GRGCYC 3 cut(s) 3385, 3419, 4069
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 6 cut(s) 299, 319, 1748, 3438, 3697, 3801
Eco53kI GAGCTC 1 cut(s) 3383
Eco57I CTGAAG 4 cut(s) 2112, 4617, 4775, 4863
Eco91I GGTNACC 2 cut(s) 991, 4702
EcoICRI GAGCTC 1 cut(s) 3383
EcoO109I RGGNCCY 2 cut(s) 8, 3697
EcoO65I GGTNACC 2 cut(s) 991, 4702
EcoRI GAATTC 3 cut(s) 642, 756, 4165
EcoRII CCWGG 8 cut(s) 987, 1342, 3991, 4576, 4693, 4730, 4801, 4901
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 6 cut(s) 504, 1200, 3445, 3831, 4117, 4216
EcoT22I ATGCAT 1 cut(s) 2946
EcoT38I GRGCYC 3 cut(s) 3385, 3419, 4069
ErhI CCWWGG 6 cut(s) 504, 1200, 3445, 3831, 4117, 4216
FalI AAGNNNNNCTT 4 cut(s) 948, 980, 3315, 3347
FaqI GGGAC 1 cut(s) 3640
FauNDI CATATG 3 cut(s) 3369, 3739, 4876
FbaI TGATCA 1 cut(s) 2119
FblI GTMKAC 2 cut(s) 2576, 3312
FriOI GRGCYC 3 cut(s) 3385, 3419, 4069
GlaI GCGC 2 cut(s) 898, 5179
GsaI CCCAGC 2 cut(s) 2637, 4072
GsuI CTGGAG 5 cut(s) 1091, 1326, 1617, 4401, 5187
HaeII RGCGCY 1 cut(s) 900
HaeIII GGCC 6 cut(s) 9, 45, 399, 2032, 4067, 4538
HapII CCGG 4 cut(s) 46, 257, 512, 2785
HhaI GCGC 2 cut(s) 899, 5180
Hin6I GCGC 2 cut(s) 897, 5178
HinP1I GCGC 2 cut(s) 897, 5178
HincII GTYRAC 4 cut(s) 265, 2098, 2263, 3591
HindII GTYRAC 4 cut(s) 265, 2098, 2263, 3591
HindIII AAGCTT 4 cut(s) 2072, 3283, 4757, 4916
HpaII CCGG 4 cut(s) 46, 257, 512, 2785
HphI GGTGA 5 cut(s) 1185, 1731, 3297, 4523, 4622
Hpy99I CGWCG 3 cut(s) 580, 2959, 3589
HpyCH4IV ACGT 6 cut(s) 1325, 2539, 2581, 2780, 2904, 4288
HpySE526I ACGT 6 cut(s) 1325, 2539, 2581, 2780, 2904, 4288
HspAI GCGC 2 cut(s) 897, 5178
Kpn2I TCCGGA 1 cut(s) 2784
KpnI GGTACC 2 cut(s) 512, 3231
Ksp22I TGATCA 1 cut(s) 2119
LguI GCTCTTC 1 cut(s) 3339
MabI ACCWGGT 2 cut(s) 987, 4901
MaeII ACGT 6 cut(s) 1325, 2539, 2581, 2780, 2904, 4288
MfeI CAATTG 1 cut(s) 2763
MflI RGATCY 3 cut(s) 1043, 1457, 5208
MhlI GDGCHC 8 cut(s) 79, 2387, 3199, 3385, 3419, 3610, 4069, 4861
MmeI TCCRAC 7 cut(s) 905, 1203, 2946, 3465, 3547, 4693, 5068
Mph1103I ATGCAT 1 cut(s) 2946
MroI TCCGGA 1 cut(s) 2784
MroXI GAANNNNTTC 5 cut(s) 960, 1084, 2238, 3529, 3870
MslI CAYNNNNRTG 9 cut(s) 312, 888, 1103, 2174, 2823, 2949, 3079, 3368, 4611
MspA1I CMGCKG 4 cut(s) 2501, 2678, 4236, 5035
MspCI CTTAAG 1 cut(s) 4930
MspI CCGG 4 cut(s) 46, 257, 512, 2785
MspR9I CCNGG 9 cut(s) 257, 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
MunI CAATTG 1 cut(s) 2763
Mva1269I GAATGC 2 cut(s) 319, 3189
MvaI CCWGG 8 cut(s) 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
NciI CCSGG 1 cut(s) 257
NcoI CCATGG 3 cut(s) 504, 1200, 4216
NdeI CATATG 3 cut(s) 3369, 3739, 4876
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 3 cut(s) 364, 2279, 2767
NsiI ATGCAT 1 cut(s) 2946
NspI RCATGY 9 cut(s) 361, 372, 1108, 1401, 1886, 2954, 3084, 4046, 5228
NspV TTCGAA 1 cut(s) 697
PaeI GCATGC 2 cut(s) 1108, 3084
PagI TCATGA 2 cut(s) 250, 4530
PaqCI CACCTGC 1 cut(s) 1106
PceI AGGCCT 2 cut(s) 399, 4538
PciI ACATGT 2 cut(s) 368, 1397
PciSI GCTCTTC 1 cut(s) 3339
PcsI WCGNNNNNNNCGW 2 cut(s) 584, 4285
PctI GAATGC 2 cut(s) 319, 3189
PdmI GAANNNNTTC 5 cut(s) 960, 1084, 2238, 3529, 3870
PflMI CCANNNNNTGG 5 cut(s) 21, 3163, 4084, 4292, 5219
PfoI TCCNGGA 2 cut(s) 1342, 3991
PinAI ACCGGT 1 cut(s) 511
Ple19I CGATCG 1 cut(s) 1573
Ppu21I YACGTR 3 cut(s) 2540, 2582, 4289
PpuMI RGGWCCY 1 cut(s) 3697
PscI ACATGT 2 cut(s) 368, 1397
PshBI ATTAAT 1 cut(s) 807
PsiI TTATAA 2 cut(s) 2604, 3968
Psp124BI GAGCTC 1 cut(s) 3385
Psp5II RGGWCCY 1 cut(s) 3697
Psp6I CCWGG 8 cut(s) 987, 1342, 3991, 4576, 4693, 4730, 4801, 4901
PspEI GGTNACC 2 cut(s) 991, 4702
PspFI CCCAGC 2 cut(s) 2633, 4068
PspGI CCWGG 8 cut(s) 987, 1342, 3991, 4576, 4693, 4730, 4801, 4901
PspOMI GGGCCC 1 cut(s) 4065
PspPI GGNCC 9 cut(s) 8, 299, 319, 1748, 3438, 3697, 3801, 4065, 4066
PspPPI RGGWCCY 1 cut(s) 3697
PstI CTGCAG 7 cut(s) 1501, 1582, 1590, 3152, 3685, 3855, 4338
PstNI CAGNNNCTG 1 cut(s) 2150
PsuI RGATCY 3 cut(s) 1043, 1457, 5208
PvuI CGATCG 1 cut(s) 1573
PvuII CAGCTG 3 cut(s) 2501, 2678, 5035
RseI CAYNNNNRTG 9 cut(s) 312, 888, 1103, 2174, 2823, 2949, 3079, 3368, 4611
SacI GAGCTC 1 cut(s) 3385
SapI GCTCTTC 1 cut(s) 3339
Sau96I GGNCC 9 cut(s) 8, 299, 319, 1748, 3438, 3697, 3801, 4065, 4066
ScaI AGTACT 4 cut(s) 331, 2712, 3064, 3170
ScrFI CCNGG 9 cut(s) 257, 989, 1344, 3993, 4578, 4695, 4732, 4803, 4903
SduI GDGCHC 8 cut(s) 79, 2387, 3199, 3385, 3419, 3610, 4069, 4861
SexAI ACCWGGT 2 cut(s) 987, 4901
SfcI CTRYAG 9 cut(s) 127, 1497, 1578, 1586, 2691, 3148, 3681, 3851, 4334
SfuI TTCGAA 1 cut(s) 697
SinI GGWCC 6 cut(s) 299, 319, 1748, 3438, 3697, 3801
SmiI ATTTAAAT 1 cut(s) 3580
SmiMI CAYNNNNRTG 9 cut(s) 312, 888, 1103, 2174, 2823, 2949, 3079, 3368, 4611
SmlI CTYRAG 3 cut(s) 2466, 4268, 4930
SmoI CTYRAG 3 cut(s) 2466, 4268, 4930
SpeI ACTAGT 1 cut(s) 2081
SphI GCATGC 2 cut(s) 1108, 3084
SseBI AGGCCT 2 cut(s) 399, 4538
SspI AATATT 3 cut(s) 2832, 2885, 3949
SstI GAGCTC 1 cut(s) 3385
StuI AGGCCT 2 cut(s) 399, 4538
StyD4I CCNGG 9 cut(s) 255, 987, 1342, 3991, 4576, 4693, 4730, 4801, 4901
StyI CCWWGG 6 cut(s) 504, 1200, 3445, 3831, 4117, 4216
SwaI ATTTAAAT 1 cut(s) 3580
TaiI ACGT 6 cut(s) 1328, 2542, 2584, 2783, 2907, 4291
TaqI TCGA 9 cut(s) 489, 582, 697, 746, 1312, 1527, 3125, 3861, 4327
TaqII GACCGA 1 cut(s) 82
TauI GCSGC 4 cut(s) 565, 568, 670, 4964
TseFI GTSAC 3 cut(s) 364, 2279, 2767
Tsp45I GTSAC 3 cut(s) 364, 2279, 2767
TspGWI ACGGA 2 cut(s) 1152, 2903
Van91I CCANNNNNTGG 5 cut(s) 21, 3163, 4084, 4292, 5219
Vha464I CTTAAG 1 cut(s) 4930
VpaK11BI GGWCC 6 cut(s) 299, 319, 1748, 3438, 3697, 3801
VspI ATTAAT 1 cut(s) 807
XbaI TCTAGA 2 cut(s) 4853, 4894
XceI RCATGY 9 cut(s) 361, 372, 1108, 1401, 1886, 2954, 3084, 4046, 5228
XcmI CCANNNNNNNNNTGG 1 cut(s) 2750
XmaJI CCTAGG 1 cut(s) 4117
XmiI GTMKAC 2 cut(s) 2576, 3312
XmnI GAANNNNTTC 5 cut(s) 960, 1084, 2238, 3529, 3870
ZrmI AGTACT 4 cut(s) 331, 2712, 3064, 3170
Zsp2I ATGCAT 1 cut(s) 2946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.