AT1G69810
WRKY Family

WRKY transcription factor 36

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008989
Physical Location & Seq
Reverse (-)
26276948 .. 26279255
2308 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69810.2

Sequence Viewer

Length: 1164 bp
ATGATCAAAGAGGAGACCGTTTCATACTTCCAAACTTTCGATGGTGTTATGGCAGAATCTGACAAGGAAGAGGAGCTTGATGCAACAAAAGCAAAGGTGGAGAAAGTTCGGGAAGAAAATGAGAAATTAAAGCTCTTACTCTCTACGATTCTTAATAATTACAACTCTCTTCAAATGCAAGTCTCTAAAGTTCTCGGACAACAACAAGGAGCTTCGTCTATGGAGCTAGACCACATTGATCGTCAAGATGAAAATAACGACTATGATGTGGATATATCACTTAGGCTTGGAAGATCAGAGCAAAAGATCTCTAAAAAGGAGGAAAATAAGGTGGACAAGATTTCTACTAAGAACGTAGAGGAGTCAAAAGATAAAAGATCTGCACTGGGTTTTGGATTTCAGATTCAAAGCTATGAAGCCTCGAAACTAGATGATCTTTGTAGGCAGGTTAAGCTTGCAAACGCTGAAAATAAATGTGTATCTTCGAGGAAAGATGTCAAATCAGTTAGAAATGAAAACCATCAAGATGTTTTGGAGGAGCATGAACAAACAGGTTTGAAGAAAACTAGGGTTTGTGTTAAAGCATCATGTGAAGACCCATCAATAAACGACGGTTGCCAGTGGAGGAAATATGGTCAAAAGACTGCGAAAACCAATCCTCTCCCACGAGCCTATTACCGTTGCTCCATGTCGTCAAATTGTCCCGTTAGAAAACAGGTGCAAAGATGTGGAGAAGAAGAAACCTCCGCTTTTATGACAACGTACGAAGGAAACCATGACCATCCTCTCCCCATGGAAGCAAGCCACATGGCAGCTGGAACTTCCGCGGCTGCCTCCTTATTACAATCTGGCTCCTCCTCTTCCTCCTCCTCCACCTCAGCGAGTCTAAGCTATTTCTTCCCTTTTCACCACTTCTCTATCTCCACCACTAACTCGCACCCGACTGTAACATTGGACCTCACACGACCAAACTATCCCAATCAGTTACCAGACGACTACCCTCTATCGTCCTCCTCTTTCAGTCTCAACTTTTCCTCTCCAGATCCTCCACCTCCATCATCGCATGACCACACCTTGAACTTTAGTGGCTTGCGAACACAAGCTCCGTTGAGTACAGATTCATTATTGGCCCGTTATCGAACGAGACTTTCCGGACAGCAATAA

Protein Analysis

387

Amino Acids

43.35

Weight (kDa)

6.04

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 145 - 204 7e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccII CGCG 1 cut(s) 827
AccIII TCCGGA 1 cut(s) 1151
AciI CCGC 3 cut(s) 747, 825, 827
AclWI GGATC 1 cut(s) 1037
AfaI GTAC 2 cut(s) 764, 1114
AgsI TTSAA 4 cut(s) 173, 407, 559, 1078
AloI GAACNNNNNNTCC 2 cut(s) 1087, 1119
AluBI AGCT 9 cut(s) 76, 133, 212, 226, 411, 454, 815, 891, 1103
AluI AGCT 9 cut(s) 76, 133, 212, 226, 411, 454, 815, 891, 1103
Alw26I GTCTC 4 cut(s) 8, 187, 1028, 1138
AlwI GGATC 1 cut(s) 1037
AlwNI CAGNNNCTG 1 cut(s) 59
Aor13HI TCCGGA 1 cut(s) 1151
AoxI GGCC 1 cut(s) 1128
ApeKI GCWGC 2 cut(s) 812, 830
AspS9I GGNCC 2 cut(s) 955, 1129
AsuHPI GGTGA 1 cut(s) 899
AvaII GGWCC 1 cut(s) 955
BauI CACGAG 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 600
BbvCI CCTCAGC 1 cut(s) 877
BbvI GCAGC 2 cut(s) 817, 824
BccI CCATC 5 cut(s) 35, 528, 607, 789, 1063
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 4 cut(s) 8, 187, 1028, 1138
BfaI CTAG 3 cut(s) 227, 428, 567
BfuAI ACCTGC 1 cut(s) 436
BglII AGATCT 2 cut(s) 306, 377
BisI GCNGC 3 cut(s) 813, 828, 831
BlsI GCNGC 3 cut(s) 814, 829, 832
Bme18I GGWCC 1 cut(s) 955
BmgT120I GGNCC 2 cut(s) 955, 1129
BmiI GGNNCC 1 cut(s) 853
BmrI ACTGGG 1 cut(s) 395
BmsI GCATC 2 cut(s) 70, 593
BmuI ACTGGG 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 1023
Bpu10I CCTNAGC 1 cut(s) 877
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 792, 825
BsaWI WCCGGW 1 cut(s) 1151
BsaXI ACNNNNNCTCC 4 cut(s) 668, 698, 1087, 1117
Bse1I ACTGG 2 cut(s) 390, 619
BseAI TCCGGA 1 cut(s) 1151
BseDI CCNNGG 2 cut(s) 792, 825
BseGI GGATG 1 cut(s) 781
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 2 cut(s) 390, 619
BseRI GAGGAG 9 cut(s) 26, 86, 374, 551, 844, 847, 856, 859, 1003
BseXI GCAGC 2 cut(s) 817, 824
BsgI GTGCAG 1 cut(s) 366
Bsh1236I CGCG 1 cut(s) 827
BshFI GGCC 1 cut(s) 1130
BsiSI CCGG 1 cut(s) 1152
BsiWI CGTACG 1 cut(s) 762
BslFI GGGAC 1 cut(s) 687
BsmAI GTCTC 4 cut(s) 8, 187, 1028, 1138
BsmFI GGGAC 1 cut(s) 687
BsnI GGCC 1 cut(s) 1130
Bso31I GGTCTC 1 cut(s) 8
Bsp13I TCCGGA 1 cut(s) 1151
Bsp143I GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
Bsp19I CCATGG 1 cut(s) 792
BspACI CCGC 3 cut(s) 747, 825, 827
BspANI GGCC 1 cut(s) 1130
BspCNI CTCAG 1 cut(s) 890
BspEI TCCGGA 1 cut(s) 1151
BspFNI CGCG 1 cut(s) 827
BspLI GGNNCC 1 cut(s) 853
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 1 cut(s) 1037
BspTNI GGTCTC 1 cut(s) 8
BsrI ACTGG 2 cut(s) 390, 619
BssECI CCNNGG 2 cut(s) 792, 825
BssMI GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 1 cut(s) 792
Bst2BI CACGAG 1 cut(s) 666
Bst4CI ACNGT 4 cut(s) 19, 614, 680, 946
Bst6I CTCTTC 3 cut(s) 63, 174, 865
BstC8I GCNNGC 3 cut(s) 456, 802, 1091
BstDEI CTNAG 4 cut(s) 281, 348, 877, 887
BstDSI CCRYGG 2 cut(s) 792, 825
BstF5I GGATG 1 cut(s) 781
BstFNI CGCG 1 cut(s) 827
BstKTI GATC 7 cut(s) 6, 241, 296, 309, 380, 436, 1045
BstMAI GTCTC 4 cut(s) 8, 187, 1028, 1138
BstMBI GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 89, 451
BstUI CGCG 1 cut(s) 827
BstV1I GCAGC 2 cut(s) 817, 824
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 3 cut(s) 306, 377, 1042
BstYI RGATCY 3 cut(s) 306, 377, 1042
BsuRI GGCC 1 cut(s) 1130
BtgI CCRYGG 2 cut(s) 792, 825
BtgZI GCGATG 1 cut(s) 1044
BtsCI GGATG 1 cut(s) 781
BtsIMutI CAGTG 2 cut(s) 383, 626
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 456, 802, 1091
CaiI CAGNNNCTG 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 955, 1129
Cfr42I CCGCGG 1 cut(s) 828
Csp6I GTAC 2 cut(s) 763, 1113
CviAII CATG 7 cut(s) 542, 588, 688, 776, 793, 808, 1064
CviQI GTAC 2 cut(s) 763, 1113
DdeI CTNAG 4 cut(s) 281, 348, 877, 887
DpnI GATC 7 cut(s) 5, 240, 295, 308, 379, 435, 1044
DpnII GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
Eam1104I CTCTTC 3 cut(s) 63, 174, 865
EarI CTCTTC 3 cut(s) 63, 174, 865
Eco130I CCWWGG 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 955
EcoT14I CCWWGG 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 792
FaeI CATG 7 cut(s) 545, 591, 691, 779, 796, 811, 1067
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FaqI GGGAC 1 cut(s) 687
FatI CATG 7 cut(s) 541, 587, 687, 775, 792, 807, 1063
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 813, 828, 831
FokI GGATG 1 cut(s) 768
Fsp4HI GCNGC 3 cut(s) 813, 828, 831
FspBI CTAG 3 cut(s) 227, 428, 567
GluI GCNGC 3 cut(s) 813, 828, 831
GsuI CTGGAG 1 cut(s) 1023
HaeIII GGCC 1 cut(s) 1130
HapII CCGG 1 cut(s) 1152
Hin1II CATG 7 cut(s) 545, 591, 691, 779, 796, 811, 1067
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 6 cut(s) 56, 148, 362, 403, 883, 1118
HpaII CCGG 1 cut(s) 1152
HphI GGTGA 1 cut(s) 899
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 61, 197, 298, 402
Hpy188III TCNNGA 5 cut(s) 110, 245, 524, 1040, 1152
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 614
HpyAV CCTTC 1 cut(s) 761
HpyCH4III ACNGT 4 cut(s) 19, 614, 680, 946
HpyCH4IV ACGT 2 cut(s) 354, 761
HpyCH4V TGCA 5 cut(s) 83, 178, 383, 458, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 451
HpyF3I CTNAG 4 cut(s) 281, 348, 877, 887
HpySE526I ACGT 2 cut(s) 354, 761
Hsp92II CATG 7 cut(s) 545, 591, 691, 779, 796, 811, 1067
Kpn2I TCCGGA 1 cut(s) 1151
Ksp22I TGATCA 1 cut(s) 3
KspI CCGCGG 1 cut(s) 828
Kzo9I GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
LmnI GCTCC 7 cut(s) 73, 209, 223, 538, 689, 857, 1108
LpnPI CCDG 9 cut(s) 371, 431, 537, 632, 701, 801, 834, 1002, 1053
Lsp1109I GCAGC 2 cut(s) 817, 824
LweI GCATC 2 cut(s) 70, 593
MaeI CTAG 3 cut(s) 227, 428, 567
MaeII ACGT 2 cut(s) 354, 761
MaeIII GTNAC 2 cut(s) 946, 984
MalI GATC 7 cut(s) 5, 240, 295, 308, 379, 435, 1044
MboI GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
MflI RGATCY 3 cut(s) 306, 377, 1042
MluCI AATT 3 cut(s) 125, 157, 697
MlyI GAGTC 2 cut(s) 371, 892
MroI TCCGGA 1 cut(s) 1151
MseI TTAA 4 cut(s) 128, 153, 450, 579
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 2 cut(s) 815, 827
MspI CCGG 1 cut(s) 1152
MvnI CGCG 1 cut(s) 827
MwoI GCNNNNNNNGC 2 cut(s) 89, 451
NcoI CCATGG 1 cut(s) 792
NdeII GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
NlaIII CATG 7 cut(s) 545, 591, 691, 779, 796, 811, 1067
NlaIV GGNNCC 1 cut(s) 853
PfeI GAWTC 4 cut(s) 56, 148, 403, 1118
Pfl23II CGTACG 1 cut(s) 762
PkrI GCNGC 3 cut(s) 814, 829, 832
PleI GAGTC 2 cut(s) 370, 891
PpsI GAGTC 2 cut(s) 370, 891
PspLI CGTACG 1 cut(s) 762
PspN4I GGNNCC 1 cut(s) 853
PspPI GGNCC 2 cut(s) 955, 1129
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 3 cut(s) 306, 377, 1042
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 2 cut(s) 764, 1114
RsaNI GTAC 2 cut(s) 763, 1113
RseI CAYNNNNRTG 1 cut(s) 525
SacII CCGCGG 1 cut(s) 828
SaqAI TTAA 4 cut(s) 128, 153, 450, 579
SatI GCNGC 3 cut(s) 813, 828, 831
Sau3AI GATC 7 cut(s) 3, 238, 293, 306, 377, 433, 1042
Sau96I GGNCC 2 cut(s) 955, 1129
SchI GAGTC 2 cut(s) 371, 892
SfaNI GCATC 2 cut(s) 70, 593
Sfr303I CCGCGG 1 cut(s) 828
SgrBI CCGCGG 1 cut(s) 828
SinI GGWCC 1 cut(s) 955
SmiMI CAYNNNNRTG 1 cut(s) 525
Sse9I AATT 3 cut(s) 125, 157, 697
SsiI CCGC 3 cut(s) 747, 825, 827
SspMI CTAG 3 cut(s) 227, 428, 567
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 4 cut(s) 19, 614, 680, 946
TaiI ACGT 2 cut(s) 357, 764
TaqI TCGA 4 cut(s) 39, 422, 485, 1138
TasI AATT 3 cut(s) 125, 157, 697
TatI WGTACW 1 cut(s) 1112
TauI GCSGC 1 cut(s) 830
TfiI GAWTC 4 cut(s) 56, 148, 403, 1118
Tru1I TTAA 4 cut(s) 128, 153, 450, 579
Tru9I TTAA 4 cut(s) 128, 153, 450, 579
TscAI CASTG 2 cut(s) 390, 626
TseI GCWGC 2 cut(s) 812, 830
TspDTI ATGAA 6 cut(s) 12, 264, 429, 528, 558, 1110
TspGWI ACGGA 1 cut(s) 1095
TspRI CASTG 2 cut(s) 390, 626
VpaK11BI GGWCC 1 cut(s) 955
XcmI CCANNNNNNNNNTGG 2 cut(s) 38, 812
XspI CTAG 3 cut(s) 227, 428, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.