Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0008989

Physical Location & Seq

Genomic Coordinates Forward (+)

27648447 .. 27651771

Gene Structure 3325 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G269200.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGCCAAAGAGGTGTTCAAAAGCAATGGAGAACAATAATACTGAGGAAAATTCTTTCCTTGGAAATACACCAGTGGAAGACAAAACTAAGAAATCTGATGATGGTCATGATGAAGAGGGTACTTCCATGGAGATGGAAATAAGCAAGTCTTCCTCATCCATGGATATCACAATACAGGGAGGACATGGGTGGCTGGCTGAACCAGAGCAAACAAACACTTTGATGCCGTCTTCTTCTGCTGGAAAAGATAGAGCCCTTCTCAAGCACCCACAGGAGAATGAGCTCCAATCAGCTAAAGCTAAAATGGGGGAGGTTAAAGAAGAGAATGAAAAGTTAAAGCTGTTACTATCGCAAATCGTGAGGAATTACCAGTCTCTTCAGATGCATTTTCTTGGTCTTCTTCAAAAAGATGAAGAAGCTAAGAAATCTATGGATCCTAGTAGTTCTGCTCGTGATCAGTCGAACGAACAAGACGAAGAAGCTGACGAACTTGTGTCCCTTAGCCTTGGAAGAAGCTCAAGCACTGATCAGCCCAGAAAGGATGAACAGATGAAAAAGACTAGCCATTTCAGTAAAAATGGGAAAGGTGACGATCAAGAAGGACTCAACGGAGCTGGACTTGCACTGGAATTGGGCTGCAGATTCGAGCCAGCTGCTGATCAATCAACTGAAGTTGTTATGAAGAATTCAAGCTCTGATAATAGCTGCGGAGACCCCAAGGAAGATGACCCGACTGAGATATGGCCACCTAGCAAGACTTTGAAGACCACAAGAAGTGGGGATGATGAGGTTTCACAACAAACCCATTTGAAGAAAGCTAGGGTTTCTGTCAGAGCTAGATGCGATGCTCCAACGATGCATGATGGATGCCAATGGAGAAAATATGGACAGAAAATAGCAAAAGGAAATCCATGCCCTCGAGCGTACTATCGCTGCACTGTCTCACCATCCTGCCCAGTGAGAAAACAGGTGCAAAGATGTGCTGATGACATGTCCATATTAATCACAACCTACGAAGGAAGCCACAACCATCCACTTCCTATGTCAGCCACAGCCATGGCCTCCACCACCTCCGCTGCGGCTTCCATGCTTCAGTCTCACTCCTCCATCTCCCAACAAGGCCACACCGCCATGGCACCCATCTCTGCCTCCACCAACCTCCAAGGACTAAACTTCAGTACAAGTACTCTCTCCCAAAATTCAAGACTACCACAACACTTCTATTTCCCCAACAGTTCAATCTCAACCACAAATTCCCACCCAACCATCACTCTTGATCTCACTGCTCCATCTCCCTCTCACTTTGGACGATTTCCTGCAGCTGTCTTCTCTTCCAACCCAAGATATCCCTCCACACGTCTCAATTTTTCCTCATCTCCCTCCTCTTCTTTAGACCACAACAACAACACATTGCAGTTGCAAGCACCTTGGAACAACAATCACACTGCAGCTGACTATTTGAACTATGGGAATAGAGTACTTAACCAAGTTGGGTCAACTCTAAACATGGGAAAGCAATCAATATTTCAAGAACCTAATAATTTGTACCAATCTTACATACAAAATCAGAAGCCTCCTCCTCCTCCTCTTCCTCCCCCTCCTCATCAGCAGTTGATGTTGACAGAGACAATAGCCTCTGCAACAAAAACAATCACATCAAACCCTAAATTCCAATCAGCATTAGCAGCTGCACTCACATCATTTGTTGGTACTAATAATGGGGGTAGTGCTACTACTGGGGTCAGAGAAAATCATCATCATCATCAGCATCAAAGTAGCACTTCTACAGAAAGTGCATCTGGGCTGAAATTGAAGTGGGCTGAATCTTTGACAACAAATCCAATCTACCCACCAAGCCCAAATGGAATAGGGTGCGCATCAAGCTACTTGAACAAATCTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

635

Amino Acids

69.14

Weight (kDa)

6.52

Isoelectric Point (pI)

53.91

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008989)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69810 AT1G69810
fragaria_vesca	FvH4_4g31790 FvH4_4g31790 FvH4_4g31790
malus_domestica	MD13G1077900.v1.1 MD16G1077700.v1.1
prunus_persica	Prupe.1G269200_v2.0.a1 Prupe.1G269200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0440391
rosa_laevigata	RLG00000006186
rosa_multiflora	Rmu_co8279817.1_g000001 Rmu_co8317961.1_g000001 Rmu_sc0000938.1_g000002 Rmu_sc0001685.1_g000047
rosa_roxburghii	Rroxscaffold_5G00381260
rosa_rugosa	Rorug04G0325900
rosa_samantha	Rh4AG377100 Rh4BG390100 Rh4CG404900 Rh4DG383800
rosa_wichuraiana	Rw4G032290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1876
AccB1I	GGYRCC	1 cut(s)	1135
AciI	CCGC	4 cut(s)	708, 1074, 1079, 1128
AclWI	GGATC	2 cut(s)	428, 441
AcoI	YGGCCR	1 cut(s)	743
AcsI	RAATTY	5 cut(s)	49, 685, 1198, 1252, 1667
AcuI	CTGAAG	4 cut(s)	362, 690, 1076, 1159
AfaI	GTAC	7 cut(s)	121, 926, 1180, 1186, 1479, 1547, 1711
AflIII	ACRYGT	2 cut(s)	990, 1355
AjiI	CACGTC	1 cut(s)	1358
AjuI	GAANNNNNNNTTGG	1 cut(s)	30
Alw21I	GWGCWC	1 cut(s)	285
Alw26I	GTCTC	6 cut(s)	378, 705, 946, 1101, 1364, 1619
AlwI	GGATC	2 cut(s)	428, 441
AlwNI	CAGNNNCTG	1 cut(s)	656
Ama87I	CYCGRG	1 cut(s)	918
AoxI	GGCC	3 cut(s)	743, 1059, 1120
ApeKI	GCWGC	9 cut(s)	636, 653, 705, 933, 1076, 1319, 1448, 1685, 1688
ApoI	RAATTY	5 cut(s)	49, 685, 1198, 1252, 1667
AseI	ATTAAT	1 cut(s)	1001
AspLEI	GCGC	1 cut(s)	1877
AsuHPI	GGTGA	2 cut(s)	599, 936
AvaI	CYCGRG	1 cut(s)	918
BalI	TGGCCA	1 cut(s)	745
BamHI	GGATCC	1 cut(s)	433
BanI	GGYRCC	1 cut(s)	1135
BanII	GRGCYC	2 cut(s)	256, 285
BauI	CACGAG	1 cut(s)	450
BbsI	GAAGAC	6 cut(s)	84, 141, 222, 389, 770, 1318
Bbv12I	GWGCWC	1 cut(s)	285
BbvI	GCAGC	9 cut(s)	623, 640, 692, 920, 1063, 1331, 1460, 1675, 1697
BccI	CCATC	9 cut(s)	95, 127, 857, 955, 1038, 1115, 1148, 1274, 1297
BceAI	ACGGC	1 cut(s)	211
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BclI	TGATCA	3 cut(s)	454, 526, 658
BcoDI	GTCTC	6 cut(s)	378, 705, 946, 1101, 1364, 1619
BfaI	CTAG	5 cut(s)	438, 561, 750, 819, 837
BfmI	CTRYAG	4 cut(s)	637, 1317, 1446, 1785
BmcAI	AGTACT	2 cut(s)	1186, 1479
BmeT110I	CYCGRG	1 cut(s)	918
BmgBI	CACGTC	1 cut(s)	1358
BmiI	GGNNCC	2 cut(s)	435, 1137
BmrI	ACTGGG	2 cut(s)	950, 1746
BmsI	GCATC	9 cut(s)	213, 372, 830, 835, 846, 857, 1777, 1805, 1886
BmuI	ACTGGG	2 cut(s)	950, 1746
BpiI	GAAGAC	6 cut(s)	84, 141, 222, 389, 770, 1318
BplI	GAGNNNNNCTC	2 cut(s)	243, 275
Bpu10I	CCTNAGC	1 cut(s)	500
BpuEI	CTTGAG	2 cut(s)	245, 502
BsaI	GGTCTC	1 cut(s)	705
BsaJI	CCNNGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
Bse1I	ACTGG	5 cut(s)	71, 370, 630, 956, 1741
Bse3DI	GCAATG	2 cut(s)	30, 1409
BseDI	CCNNGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
BseGI	GGATG	6 cut(s)	155, 547, 787, 872, 947, 1030
BseMI	GCAATG	2 cut(s)	30, 1409
BseMII	CTCAG	2 cut(s)	33, 726
BseNI	ACTGG	5 cut(s)	71, 370, 630, 956, 1741
BseRI	GAGGAG	7 cut(s)	1093, 1372, 1566, 1569, 1572, 1575, 1590
BseXI	GCAGC	9 cut(s)	623, 640, 692, 920, 1063, 1331, 1460, 1675, 1697
BsgI	GTGCAG	2 cut(s)	919, 1674
BshFI	GGCC	3 cut(s)	745, 1061, 1122
BshNI	GGYRCC	1 cut(s)	1135
BsiHKAI	GWGCWC	1 cut(s)	285
BsiHKCI	CYCGRG	1 cut(s)	918
BslFI	GGGAC	1 cut(s)	481
BsmAI	GTCTC	6 cut(s)	378, 705, 946, 1101, 1364, 1619
BsmBI	CGTCTC	1 cut(s)	1364
BsmFI	GGGAC	1 cut(s)	481
BsnI	GGCC	3 cut(s)	745, 1061, 1122
Bso31I	GGTCTC	1 cut(s)	705
BsoBI	CYCGRG	1 cut(s)	918
Bsp1286I	GDGCHC	2 cut(s)	256, 285
Bsp143I	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
Bsp19I	CCATGG	4 cut(s)	126, 159, 1056, 1131
BspACI	CCGC	4 cut(s)	708, 1074, 1079, 1128
BspANI	GGCC	3 cut(s)	745, 1061, 1122
BspCNI	CTCAG	2 cut(s)	34, 727
BspHI	TCATGA	1 cut(s)	106
BspLI	GGNNCC	2 cut(s)	435, 1137
BspMAI	CTGCAG	3 cut(s)	641, 1321, 1450
BspPI	GGATC	2 cut(s)	428, 441
BspT107I	GGYRCC	1 cut(s)	1135
BspTNI	GGTCTC	1 cut(s)	705
BsrDI	GCAATG	2 cut(s)	30, 1409
BsrI	ACTGG	5 cut(s)	71, 370, 630, 956, 1741
BssECI	CCNNGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
BssMI	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
BssSI	CACGAG	1 cut(s)	450
BssT1I	CCWWGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
Bst2BI	CACGAG	1 cut(s)	450
Bst4CI	ACNGT	2 cut(s)	940, 1235
Bst6I	CTCTTC	6 cut(s)	108, 315, 381, 1336, 1390, 1593
BstC8I	GCNNGC	3 cut(s)	195, 651, 1422
BstDEI	CTNAG	5 cut(s)	42, 87, 420, 500, 735
BstDSI	CCRYGG	4 cut(s)	126, 159, 1056, 1131
BstF5I	GGATG	6 cut(s)	155, 547, 787, 872, 947, 1030
BstHHI	GCGC	1 cut(s)	1877
BstKTI	GATC	6 cut(s)	436, 457, 529, 595, 661, 1279
BstMAI	GTCTC	6 cut(s)	378, 705, 946, 1101, 1364, 1619
BstMBI	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
BstMWI	GCNNNNNNNGC	3 cut(s)	620, 1685, 1881
BstNSI	RCATGY	1 cut(s)	994
BstSFI	CTRYAG	4 cut(s)	637, 1317, 1446, 1785
BstV1I	GCAGC	9 cut(s)	623, 640, 692, 920, 1063, 1331, 1460, 1675, 1697
BstV2I	GAAGAC	6 cut(s)	84, 141, 222, 389, 770, 1318
BstX2I	RGATCY	1 cut(s)	433
BstXI	CCANNNNNNTGG	2 cut(s)	133, 1057
BstYI	RGATCY	1 cut(s)	433
BsuRI	GGCC	3 cut(s)	745, 1061, 1122
BtgI	CCRYGG	4 cut(s)	126, 159, 1056, 1131
BtgZI	GCGATG	1 cut(s)	858
BtrI	CACGTC	1 cut(s)	1358
BtsCI	GGATG	6 cut(s)	155, 547, 787, 872, 947, 1030
BtsI	GCAGTG	2 cut(s)	1281, 1443
BtsIMutI	CAGTG	7 cut(s)	78, 522, 623, 936, 963, 1281, 1443
Cac8I	GCNNGC	3 cut(s)	195, 651, 1422
CaiI	CAGNNNCTG	1 cut(s)	656
CciI	TCATGA	1 cut(s)	106
CfoI	GCGC	1 cut(s)	1877
Csp6I	GTAC	7 cut(s)	120, 925, 1179, 1185, 1478, 1546, 1710
CviQI	GTAC	7 cut(s)	120, 925, 1179, 1185, 1478, 1546, 1710
DdeI	CTNAG	5 cut(s)	42, 87, 420, 500, 735
DpnI	GATC	6 cut(s)	435, 456, 528, 594, 660, 1278
DpnII	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
EaeI	YGGCCR	1 cut(s)	743
Eam1104I	CTCTTC	6 cut(s)	108, 315, 381, 1336, 1390, 1593
EarI	CTCTTC	6 cut(s)	108, 315, 381, 1336, 1390, 1593
Ecl136II	GAGCTC	1 cut(s)	283
Eco130I	CCWWGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
Eco24I	GRGCYC	2 cut(s)	256, 285
Eco31I	GGTCTC	1 cut(s)	705
Eco32I	GATATC	2 cut(s)	166, 1346
Eco53kI	GAGCTC	1 cut(s)	283
Eco57I	CTGAAG	4 cut(s)	362, 690, 1076, 1159
Eco88I	CYCGRG	1 cut(s)	918
EcoICRI	GAGCTC	1 cut(s)	283
EcoRI	GAATTC	1 cut(s)	685
EcoRV	GATATC	2 cut(s)	166, 1346
EcoT14I	CCWWGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
EcoT22I	ATGCAT	2 cut(s)	387, 861
EcoT38I	GRGCYC	2 cut(s)	256, 285
ErhI	CCWWGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
Esp3I	CGTCTC	1 cut(s)	1364
FalI	AAGNNNNNCTT	4 cut(s)	133, 165, 1765, 1797
FaqI	GGGAC	1 cut(s)	481
FbaI	TGATCA	3 cut(s)	454, 526, 658
FokI	GGATG	6 cut(s)	142, 554, 794, 879, 934, 1017
FriOI	GRGCYC	2 cut(s)	256, 285
FspAI	RTGCGCAY	1 cut(s)	1876
FspBI	CTAG	5 cut(s)	438, 561, 750, 819, 837
FspI	TGCGCA	1 cut(s)	1876
GlaI	GCGC	1 cut(s)	1876
HaeIII	GGCC	3 cut(s)	745, 1061, 1122
HhaI	GCGC	1 cut(s)	1877
Hin6I	GCGC	1 cut(s)	1875
HinP1I	GCGC	1 cut(s)	1875
HincII	GTYRAC	2 cut(s)	1497, 1620
HindII	GTYRAC	2 cut(s)	1497, 1620
HinfI	GANTC	3 cut(s)	603, 642, 1823
HphI	GGTGA	2 cut(s)	599, 936
Hpy166II	GTNNAC	2 cut(s)	1497, 1620
Hpy188I	TCNGA	6 cut(s)	97, 381, 697, 833, 1569, 1745
Hpy188III	TCNNGA	8 cut(s)	107, 358, 452, 596, 1203, 1274, 1529, 1902
Hpy8I	GTNNAC	2 cut(s)	1497, 1620
HpyAV	CCTTC	3 cut(s)	266, 593, 1010
HpyCH4III	ACNGT	2 cut(s)	940, 1235
HpyCH4IV	ACGT	1 cut(s)	1357
HpyF10VI	GCNNNNNNNGC	3 cut(s)	620, 1685, 1881
HpyF3I	CTNAG	5 cut(s)	42, 87, 420, 500, 735
HpySE526I	ACGT	1 cut(s)	1357
HspAI	GCGC	1 cut(s)	1875
Ksp22I	TGATCA	3 cut(s)	454, 526, 658
Kzo9I	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
LmnI	GCTCC	4 cut(s)	288, 611, 853, 1291
Lsp1109I	GCAGC	9 cut(s)	623, 640, 692, 920, 1063, 1331, 1460, 1675, 1697
LweI	GCATC	9 cut(s)	213, 372, 830, 835, 846, 857, 1777, 1805, 1886
MaeI	CTAG	5 cut(s)	438, 561, 750, 819, 837
MaeII	ACGT	1 cut(s)	1357
MaeIII	GTNAC	2 cut(s)	342, 587
MalI	GATC	6 cut(s)	435, 456, 528, 594, 660, 1278
MboI	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
MflI	RGATCY	1 cut(s)	433
MhlI	GDGCHC	2 cut(s)	256, 285
MlsI	TGGCCA	1 cut(s)	745
MluNI	TGGCCA	1 cut(s)	745
MlyI	GAGTC	1 cut(s)	597
MmeI	TCCRAC	2 cut(s)	875, 1359
Mox20I	TGGCCA	1 cut(s)	745
Mph1103I	ATGCAT	2 cut(s)	387, 861
MscI	TGGCCA	1 cut(s)	745
MseI	TTAA	4 cut(s)	315, 335, 1001, 1482
MslI	CAYNNNNRTG	4 cut(s)	131, 221, 1055, 1130
Msp20I	TGGCCA	1 cut(s)	745
MspA1I	CMGCKG	5 cut(s)	653, 1076, 1322, 1451, 1688
MwoI	GCNNNNNNNGC	3 cut(s)	620, 1685, 1881
NcoI	CCATGG	4 cut(s)	126, 159, 1056, 1131
NdeII	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
NlaIV	GGNNCC	2 cut(s)	435, 1137
NmuCI	GTSAC	1 cut(s)	587
NsbI	TGCGCA	1 cut(s)	1876
NsiI	ATGCAT	2 cut(s)	387, 861
NspI	RCATGY	1 cut(s)	994
PaeR7I	CTCGAG	1 cut(s)	918
PagI	TCATGA	1 cut(s)	106
PciI	ACATGT	1 cut(s)	990
PcsI	WCGNNNNNNNCGW	1 cut(s)	471
PfeI	GAWTC	2 cut(s)	642, 1823
PleI	GAGTC	1 cut(s)	597
PpsI	GAGTC	1 cut(s)	597
PscI	ACATGT	1 cut(s)	990
PshBI	ATTAAT	1 cut(s)	1001
Psp124BI	GAGCTC	1 cut(s)	285
PspN4I	GGNNCC	2 cut(s)	435, 1137
PspXI	VCTCGAGB	1 cut(s)	918
PstI	CTGCAG	3 cut(s)	641, 1321, 1450
PstNI	CAGNNNCTG	1 cut(s)	656
PsuI	RGATCY	1 cut(s)	433
PvuII	CAGCTG	4 cut(s)	653, 1322, 1451, 1688
RsaI	GTAC	7 cut(s)	121, 926, 1180, 1186, 1479, 1547, 1711
RsaNI	GTAC	7 cut(s)	120, 925, 1179, 1185, 1478, 1546, 1710
RseI	CAYNNNNRTG	4 cut(s)	131, 221, 1055, 1130
SacI	GAGCTC	1 cut(s)	285
SaqAI	TTAA	4 cut(s)	315, 335, 1001, 1482
Sau3AI	GATC	6 cut(s)	433, 454, 526, 592, 658, 1276
ScaI	AGTACT	2 cut(s)	1186, 1479
SchI	GAGTC	1 cut(s)	597
SduI	GDGCHC	2 cut(s)	256, 285
SfaNI	GCATC	9 cut(s)	213, 372, 830, 835, 846, 857, 1777, 1805, 1886
SfcI	CTRYAG	4 cut(s)	637, 1317, 1446, 1785
Sfr274I	CTCGAG	1 cut(s)	918
SlaI	CTCGAG	1 cut(s)	918
SmiMI	CAYNNNNRTG	4 cut(s)	131, 221, 1055, 1130
SmlI	CTYRAG	3 cut(s)	260, 517, 918
SmoI	CTYRAG	3 cut(s)	260, 517, 918
SsiI	CCGC	4 cut(s)	708, 1074, 1079, 1128
SspI	AATATT	1 cut(s)	1524
SspMI	CTAG	5 cut(s)	438, 561, 750, 819, 837
SstI	GAGCTC	1 cut(s)	285
StyI	CCWWGG	9 cut(s)	58, 126, 159, 505, 717, 1056, 1131, 1162, 1427
TaaI	ACNGT	2 cut(s)	940, 1235
TaiI	ACGT	1 cut(s)	1360
TaqI	TCGA	3 cut(s)	461, 645, 919
TatI	WGTACW	3 cut(s)	1178, 1184, 1477
TauI	GCSGC	1 cut(s)	1082
TfiI	GAWTC	2 cut(s)	642, 1823
Tru1I	TTAA	4 cut(s)	315, 335, 1001, 1482
Tru9I	TTAA	4 cut(s)	315, 335, 1001, 1482
TscAI	CASTG	7 cut(s)	78, 529, 630, 943, 963, 1288, 1450
TseFI	GTSAC	1 cut(s)	587
TseI	GCWGC	9 cut(s)	636, 653, 705, 933, 1076, 1319, 1448, 1685, 1688
Tsp45I	GTSAC	1 cut(s)	587
TspDTI	ATGAA	6 cut(s)	126, 342, 426, 558, 566, 695
TspGWI	ACGGA	1 cut(s)	624
TspRI	CASTG	7 cut(s)	78, 529, 630, 943, 963, 1288, 1450
VspI	ATTAAT	1 cut(s)	1001
XapI	RAATTY	5 cut(s)	49, 685, 1198, 1252, 1667
XceI	RCATGY	1 cut(s)	994
XhoI	CTCGAG	1 cut(s)	918
XspI	CTAG	5 cut(s)	438, 561, 750, 819, 837
ZrmI	AGTACT	2 cut(s)	1186, 1479
Zsp2I	ATGCAT	2 cut(s)	387, 861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.