MD13G1077900.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008989
Physical Location & Seq
Reverse (-)
5478407 .. 5482438
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1077900.v1.1.491

Sequence Viewer

Length: 1716 bp
ATGGAGAACGATAATACTCATGAGGAATCGTCTTCTCTTGGCAAAACAATTTTGGAAGACAAAACTGTGAAATCTGATGGTGATCATGAAGAGGGTACTCCCATGGAGATAACCAAGGATGAGCTGCAATCAGCAAAAGCAGAAATGGTAGAGGCTACGGAAGAAAACGAAAGGCTAAAGCTGTTATTATCCCAAATCTCTAAGGATTACCAGTCTCTGCAGATGCATTTTCATGGCCTTCTTCAAAATGGAGGAGAAACTAAGAAATGTACGGATACTAGTGGTAGTACTGCTCTTGATCTTACGCATGAGCAAAATACTGAAGAAGCTGACCTAGTGTCTCTTAGTCTTGGGAGAACCTCAAGTAGTATTGATCAGCCCAGAAAGGATGGTCGGATAAAAAAGATTAGCCAACTAAATACTAATGGAAAAGATGATGACGATGATAATGGAATGCTGAATGGAGCTGGACTTGCACTGGAGTTGGGCTGCAAATTTGAGCCGGCTGCTGATCAGTCAACTGATAATAGCTCTGGAGGCCCGAAGGAAGACGACCTGGCTGAGATATGGCCGCCTAGTAAGACGTTGAAGACAACGAGAAGTAGAGATGATGAGGTTTCGCAACAAACCCATTTGAAGAAAGCTAGGGTTTCTGTCAGAGTTAGATGCGATGATCACACGATTTATGATGGATGTCAATGGAGAAAATATGGACAGAAAATAGCAAAAGGAAATCCATGCCCTAGAGCGTACTATCGTTGCAGTGTCTCAGCATCTTGCCCTGTGAGAAAACAGGTGCAAAGAAGTTCCGAGGACATGTCCATACTAACCACAACCTACGAAGGAAGCCACAACCACCCACTTCCCATGTCAGCCACAGCCATGGCCTCCACCACCTCCGCCGCTGCTTCCATGCTTCAGTCTCACTCATCCACCTCCCAACAAGACCTCATCAACTCCACCACCGCTCCCATCTCTGCCTCCACTAATCTCGAAGGAGTAAACTTCAGTGGTACTAGTACTCTCTCTCAAAATTCAAGGCTACCACAGAAACACTTCTACTTCCCCAACAGTTCGATCTCAACCAACAATTCCCACCCAACTATCACTCTTGATCTCACCGTCCCATCTCCTTCTCATTTCGGAATATTCCCCTCCGCGTCGGGGAGCAGCTTTTCTTCCAACCCAAGATATCCCTCAACATCTCTCAACTTTTCGTCTTCTTTAGACCACAATAACGCATTGCAATTGCAATCTCCTTGGAACAACATCAATCACACAGCTTCTGGATATTTCAACTATGGGAATAGAATTAATCAAGTTGGATCAGCCCTAAACGTTGGAAAGCAACCAATATTTCAAGAACATAACATATTGTACCAATCCTATTCCTACATACAAAATAAAAAACCCTCTCCTCCTCCTCTTCATCATCCTCAGATGTTCACCGATTCAATGGCCACCGCCACAAAGGCAATCACATCGAATCCAAATTTTCAATCGGCTTTGGCAGCTGCACTCACATCATTTGTTGGCAATGGGAGTACAAGTAGTACCACTGGGATCAGAGAAAGTCATCTAAGTACTGGGACTATTGTAGAAAGTGCTTCTGGGCTGAAATTGAAGCGGGGTGAGTCGTTGACGTCGAATTCAATATTCCCATCAGGCCAAAATGGAATAGGGTGTGCATCAAGCTACTTGAACGAATCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

572

Amino Acids

61.85

Weight (kDa)

5.95

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 230 - 287 9.7e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1646
AccBSI CCGCTC 1 cut(s) 968
AccII CGCG 1 cut(s) 1160
AciI CCGC 7 cut(s) 572, 900, 903, 966, 1158, 1464, 1627
AclI AACGTT 1 cut(s) 1338
AclWI GGATC 2 cut(s) 1333, 1571
AcoI YGGCCR 2 cut(s) 569, 1458
AcsI RAATTY 4 cut(s) 494, 1033, 1492, 1648
AcuI CTGAAG 3 cut(s) 342, 902, 991
AcyI GRCGYC 1 cut(s) 1643
AfiI CCNNNNNNNGG 1 cut(s) 1164
AflIII ACRYGT 1 cut(s) 816
AhdI GACNNNNNGTC 1 cut(s) 337
AhlI ACTAGT 2 cut(s) 278, 1016
AjnI CCWGG 1 cut(s) 555
Alw26I GTCTC 4 cut(s) 219, 345, 772, 927
AlwI GGATC 2 cut(s) 1333, 1571
AlwNI CAGNNNCTG 2 cut(s) 217, 1286
AoxI GGCC 6 cut(s) 235, 538, 569, 885, 1458, 1666
ApeKI GCWGC 7 cut(s) 124, 489, 506, 905, 1170, 1511, 1514
ApoI RAATTY 4 cut(s) 494, 1033, 1492, 1648
AseI ATTAAT 1 cut(s) 1314
Asp700I GAANNNNTTC 1 cut(s) 1055
AspS9I GGNCC 1 cut(s) 539
AsuHPI GGTGA 4 cut(s) 92, 1111, 1438, 1643
BalI TGGCCA 1 cut(s) 1460
BbsI GAAGAC 5 cut(s) 24, 63, 555, 596, 1212
BbvI GCAGC 7 cut(s) 111, 476, 493, 892, 1182, 1501, 1523
BccI CCATC 6 cut(s) 71, 383, 683, 980, 1135, 1669
BcgI CGANNNNNNTGC 2 cut(s) 1464, 1498
BciT130I CCWGG 1 cut(s) 557
BciVI GTATCC 1 cut(s) 268
BclI TGATCA 4 cut(s) 82, 373, 511, 673
BcoDI GTCTC 4 cut(s) 219, 345, 772, 927
BcuI ACTAGT 2 cut(s) 278, 1016
BfaI CTAG 6 cut(s) 279, 335, 576, 645, 744, 1017
BfmI CTRYAG 1 cut(s) 218
BfuI GTATCC 1 cut(s) 268
BglI GCCNNNNNGGC 1 cut(s) 1472
BisI GCNGC 9 cut(s) 125, 490, 507, 572, 903, 906, 1171, 1512, 1515
BlsI GCNGC 9 cut(s) 126, 491, 508, 573, 904, 907, 1172, 1513, 1516
BmcAI AGTACT 3 cut(s) 289, 1021, 1585
Bme1390I CCNGG 1 cut(s) 557
BmeRI GACNNNNNGTC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 539
BmrFI CCNGG 1 cut(s) 557
BmrI ACTGGG 2 cut(s) 1569, 1596
BmsI GCATC 4 cut(s) 213, 656, 782, 1697
BmuI ACTGGG 2 cut(s) 1569, 1596
BpiI GAAGAC 5 cut(s) 24, 63, 555, 596, 1212
BpmI CTGGAG 2 cut(s) 500, 555
BpuEI CTTGAG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 1643
BsaJI CCNNGG 5 cut(s) 102, 114, 810, 882, 1259
BsaXI ACNNNNNCTCC 4 cut(s) 952, 982, 1534, 1564
Bsc4I CCNNNNNNNGG 1 cut(s) 1164
Bse118I RCCGGY 1 cut(s) 502
Bse1I ACTGG 4 cut(s) 211, 483, 1564, 1591
Bse3DI GCAATG 2 cut(s) 1241, 1543
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 5 cut(s) 102, 114, 810, 882, 1259
BseGI GGATG 5 cut(s) 124, 394, 698, 929, 1432
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 1164
BseMI GCAATG 2 cut(s) 1241, 1543
BseMII CTCAG 3 cut(s) 552, 783, 1451
BseNI ACTGG 4 cut(s) 211, 483, 1564, 1591
BseRI GAGGAG 4 cut(s) 267, 1407, 1410, 1413
BseXI GCAGC 7 cut(s) 111, 476, 493, 892, 1182, 1501, 1523
BsgI GTGCAG 1 cut(s) 1500
Bsh1236I CGCG 1 cut(s) 1160
BshFI GGCC 6 cut(s) 237, 540, 571, 887, 1460, 1668
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 2 cut(s) 1109, 1603
BslI CCNNNNNNNGG 1 cut(s) 1164
BsmAI GTCTC 4 cut(s) 219, 345, 772, 927
BsmFI GGGAC 2 cut(s) 1109, 1603
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 6 cut(s) 237, 540, 571, 887, 1460, 1668
Bsp143I GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
Bsp19I CCATGG 2 cut(s) 102, 882
BspACI CCGC 7 cut(s) 572, 900, 903, 966, 1158, 1464, 1627
BspANI GGCC 6 cut(s) 237, 540, 571, 887, 1460, 1668
BspCNI CTCAG 3 cut(s) 553, 782, 1450
BspFNI CGCG 1 cut(s) 1160
BspHI TCATGA 2 cut(s) 19, 85
BspMAI CTGCAG 1 cut(s) 222
BspPI GGATC 2 cut(s) 1333, 1571
BsrBI CCGCTC 1 cut(s) 968
BsrDI GCAATG 2 cut(s) 1241, 1543
BsrFI RCCGGY 1 cut(s) 502
BsrI ACTGG 4 cut(s) 211, 483, 1564, 1591
BssAI RCCGGY 1 cut(s) 502
BssECI CCNNGG 5 cut(s) 102, 114, 810, 882, 1259
BssMI GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
BssNI GRCGYC 1 cut(s) 1643
BssT1I CCWWGG 4 cut(s) 102, 114, 882, 1259
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 3 cut(s) 67, 1073, 1123
Bst6I CTCTTC 2 cut(s) 84, 1431
BstACI GRCGYC 1 cut(s) 1643
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 7 cut(s) 201, 261, 344, 561, 769, 1437, 1580
BstDSI CCRYGG 2 cut(s) 102, 882
BstF5I GGATG 5 cut(s) 124, 394, 698, 929, 1432
BstFNI CGCG 1 cut(s) 1160
BstKTI GATC 9 cut(s) 85, 301, 376, 514, 676, 1080, 1117, 1328, 1566
BstMAI GTCTC 4 cut(s) 219, 345, 772, 927
BstMBI GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
BstMWI GCNNNNNNNGC 4 cut(s) 473, 537, 1472, 1511
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 820
BstSCI CCNGG 1 cut(s) 555
BstSFI CTRYAG 1 cut(s) 218
BstUI CGCG 1 cut(s) 1160
BstV1I GCAGC 7 cut(s) 111, 476, 493, 892, 1182, 1501, 1523
BstV2I GAAGAC 5 cut(s) 24, 63, 555, 596, 1212
BstXI CCANNNNNNTGG 1 cut(s) 883
BsuI GTATCC 1 cut(s) 268
BsuRI GGCC 6 cut(s) 237, 540, 571, 887, 1460, 1668
BtgI CCRYGG 2 cut(s) 102, 882
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 5 cut(s) 124, 394, 698, 929, 1432
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 4 cut(s) 476, 769, 1015, 1557
Cac8I GCNNGC 1 cut(s) 504
CaiI CAGNNNCTG 2 cut(s) 217, 1286
CciI TCATGA 2 cut(s) 19, 85
Cfr10I RCCGGY 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 539
CseI GACGC 1 cut(s) 1149
DdeI CTNAG 7 cut(s) 201, 261, 344, 561, 769, 1437, 1580
DpnI GATC 9 cut(s) 84, 300, 375, 513, 675, 1079, 1116, 1327, 1565
DpnII GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
DriI GACNNNNNGTC 1 cut(s) 337
EaeI YGGCCR 2 cut(s) 569, 1458
Eam1104I CTCTTC 2 cut(s) 84, 1431
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 2 cut(s) 84, 1431
EciI GGCGGA 1 cut(s) 889
Eco130I CCWWGG 4 cut(s) 102, 114, 882, 1259
Eco32I GATATC 1 cut(s) 1193
Eco57I CTGAAG 3 cut(s) 342, 902, 991
EcoRI GAATTC 1 cut(s) 1648
EcoRII CCWGG 1 cut(s) 555
EcoRV GATATC 1 cut(s) 1193
EcoT14I CCWWGG 4 cut(s) 102, 114, 882, 1259
EcoT22I ATGCAT 1 cut(s) 228
ErhI CCWWGG 4 cut(s) 102, 114, 882, 1259
FaqI GGGAC 2 cut(s) 1109, 1603
FauI CCCGC 1 cut(s) 1620
FbaI TGATCA 4 cut(s) 82, 373, 511, 673
Fnu4HI GCNGC 9 cut(s) 125, 490, 507, 572, 903, 906, 1171, 1512, 1515
FokI GGATG 5 cut(s) 131, 401, 705, 916, 1419
Fsp4HI GCNGC 9 cut(s) 125, 490, 507, 572, 903, 906, 1171, 1512, 1515
FspBI CTAG 6 cut(s) 279, 335, 576, 645, 744, 1017
GluI GCNGC 9 cut(s) 125, 490, 507, 572, 903, 906, 1171, 1512, 1515
GsuI CTGGAG 2 cut(s) 500, 555
HaeIII GGCC 6 cut(s) 237, 540, 571, 887, 1460, 1668
HapII CCGG 1 cut(s) 503
HgaI GACGC 1 cut(s) 1149
Hin1I GRCGYC 1 cut(s) 1643
HincII GTYRAC 2 cut(s) 519, 1641
HindII GTYRAC 2 cut(s) 519, 1641
HinfI GANTC 5 cut(s) 26, 1451, 1486, 1634, 1706
HpaII CCGG 1 cut(s) 503
HphI GGTGA 4 cut(s) 92, 1111, 1438, 1643
Hpy166II GTNNAC 4 cut(s) 519, 1003, 1446, 1641
Hpy188I TCNGA 7 cut(s) 76, 396, 659, 811, 1145, 1440, 1568
Hpy188III TCNNGA 8 cut(s) 20, 86, 296, 534, 992, 1112, 1287, 1361
Hpy8I GTNNAC 4 cut(s) 519, 1003, 1446, 1641
Hpy99I CGWCG 2 cut(s) 1165, 1648
HpyAV CCTTC 5 cut(s) 248, 538, 836, 989, 1143
HpyCH4III ACNGT 3 cut(s) 67, 1073, 1123
HpyCH4IV ACGT 3 cut(s) 584, 1338, 1643
HpyF10VI GCNNNNNNNGC 4 cut(s) 473, 537, 1472, 1511
HpyF3I CTNAG 7 cut(s) 201, 261, 344, 561, 769, 1437, 1580
HpySE526I ACGT 3 cut(s) 584, 1338, 1643
Hsp92I GRCGYC 1 cut(s) 1643
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Ksp22I TGATCA 4 cut(s) 82, 373, 511, 673
Kzo9I GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
LmnI GCTCC 3 cut(s) 464, 973, 1167
Lsp1109I GCAGC 7 cut(s) 111, 476, 493, 892, 1182, 1501, 1523
LweI GCATC 4 cut(s) 213, 656, 782, 1697
MaeI CTAG 6 cut(s) 279, 335, 576, 645, 744, 1017
MaeII ACGT 3 cut(s) 584, 1338, 1643
MalI GATC 9 cut(s) 84, 300, 375, 513, 675, 1079, 1116, 1327, 1565
MbiI CCGCTC 1 cut(s) 968
MboI GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
MfeI CAATTG 1 cut(s) 1247
MlsI TGGCCA 1 cut(s) 1460
MluCI AATT 9 cut(s) 48, 494, 1033, 1090, 1247, 1311, 1492, 1619, 1648
MluNI TGGCCA 1 cut(s) 1460
MlyI GAGTC 1 cut(s) 1643
MmeI TCCRAC 4 cut(s) 374, 1206, 1303, 1321
Mox20I TGGCCA 1 cut(s) 1460
Mph1103I ATGCAT 1 cut(s) 228
MroNI GCCGGC 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 1460
MseI TTAA 1 cut(s) 1314
MslI CAYNNNNRTG 2 cut(s) 231, 881
Msp20I TGGCCA 1 cut(s) 1460
MspA1I CMGCKG 2 cut(s) 905, 1514
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 1 cut(s) 557
MunI CAATTG 1 cut(s) 1247
Mva1269I GAATGC 1 cut(s) 459
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 1 cut(s) 1160
MwoI GCNNNNNNNGC 4 cut(s) 473, 537, 1472, 1511
NaeI GCCGGC 1 cut(s) 504
NcoI CCATGG 2 cut(s) 102, 882
NdeII GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
NgoMIV GCCGGC 1 cut(s) 502
NsiI ATGCAT 1 cut(s) 228
NspI RCATGY 1 cut(s) 820
PagI TCATGA 2 cut(s) 19, 85
PciI ACATGT 1 cut(s) 816
PcsI WCGNNNNNNNCGW 1 cut(s) 1643
PctI GAATGC 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 504
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 4 cut(s) 26, 1451, 1486, 1706
PkrI GCNGC 9 cut(s) 126, 491, 508, 573, 904, 907, 1172, 1513, 1516
PleI GAGTC 1 cut(s) 1642
PpsI GAGTC 1 cut(s) 1642
PscI ACATGT 1 cut(s) 816
PshBI ATTAAT 1 cut(s) 1314
Psp1406I AACGTT 1 cut(s) 1338
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspPI GGNCC 1 cut(s) 539
PstI CTGCAG 1 cut(s) 222
PstNI CAGNNNCTG 2 cut(s) 217, 1286
PvuII CAGCTG 1 cut(s) 1514
RseI CAYNNNNRTG 2 cut(s) 231, 881
SaqAI TTAA 1 cut(s) 1314
SatI GCNGC 9 cut(s) 125, 490, 507, 572, 903, 906, 1171, 1512, 1515
Sau3AI GATC 9 cut(s) 82, 298, 373, 511, 673, 1077, 1114, 1325, 1563
Sau96I GGNCC 1 cut(s) 539
ScaI AGTACT 3 cut(s) 289, 1021, 1585
SchI GAGTC 1 cut(s) 1643
ScrFI CCNGG 1 cut(s) 557
SfaNI GCATC 4 cut(s) 213, 656, 782, 1697
SfcI CTRYAG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 231, 881
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SpeI ACTAGT 2 cut(s) 278, 1016
Sse9I AATT 9 cut(s) 48, 494, 1033, 1090, 1247, 1311, 1492, 1619, 1648
SsiI CCGC 7 cut(s) 572, 900, 903, 966, 1158, 1464, 1627
SspI AATATT 3 cut(s) 1149, 1356, 1656
SspMI CTAG 6 cut(s) 279, 335, 576, 645, 744, 1017
StyD4I CCNGG 1 cut(s) 555
StyI CCWWGG 4 cut(s) 102, 114, 882, 1259
TaaI ACNGT 3 cut(s) 67, 1073, 1123
TaiI ACGT 3 cut(s) 587, 1341, 1646
TaqI TCGA 4 cut(s) 993, 1076, 1484, 1646
TasI AATT 9 cut(s) 48, 494, 1033, 1090, 1247, 1311, 1492, 1619, 1648
TatI WGTACW 4 cut(s) 287, 1019, 1544, 1583
TauI GCSGC 2 cut(s) 574, 905
TfiI GAWTC 4 cut(s) 26, 1451, 1486, 1706
Tru1I TTAA 1 cut(s) 1314
Tru9I TTAA 1 cut(s) 1314
TscAI CASTG 4 cut(s) 483, 769, 1015, 1564
TseI GCWGC 7 cut(s) 124, 489, 506, 905, 1170, 1511, 1514
TspDTI ATGAA 3 cut(s) 102, 221, 1418
TspGWI ACGGA 2 cut(s) 173, 287
TspRI CASTG 4 cut(s) 483, 769, 1015, 1564
VspI ATTAAT 1 cut(s) 1314
XapI RAATTY 4 cut(s) 494, 1033, 1492, 1648
XceI RCATGY 1 cut(s) 820
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 6 cut(s) 279, 335, 576, 645, 744, 1017
ZraI GACGTC 1 cut(s) 1644
ZrmI AGTACT 3 cut(s) 289, 1021, 1585
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.