MD16G1077700.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008989
Physical Location & Seq
Reverse (-)
5438696 .. 5444408
5713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1077700.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGAGAATAATAATACTCATGAGGAAAAGTCTTTTCTTGGCAAAACAGATGTGGAAGACAATACTGTGAAATCGGATGGTGATCATGAAGAGGGTACTCCCATGGAGACAACCAAGGATGAAATGCAATCAGCAAAAGCAGAAATGGTCAAGGTTAGGGAAGAAAACGAAAAGCTAAAGCTGTTATTATCCCAAATCTCGAGGGATTACCAGTATCTGCAGATGCATCTTCATGGCCTTCTTCAAAATGAAGAAGAAACTAAGAAATGTACCGATGCTAGTAGTAGCAGTACTGCTCGTGATTTTACGCATGAGCAAAATATTGAAGAAGCTGATGACCTAGTATCTCTTAGCCTTGGGAGAACCTCAAGTAGTATTGATCAGCCCAGAAAGGATGGTCGGAGCCAGTTGAGTTCTACTAATGGAAAAGATGACGACGATGATGATAAAATGCTGCATGGAGCTGGACTTGCACTGGAGTTGGGCTGCAGATCTGAACCAGCTGCTGATCAGTCAACTGATAATAGCTCTGGAGGCCCGAAGGAAGACGATCAGACTGAGATATGGCCGCCTAGTAAGACGTTGAAGACGACGAGAAGTGGAGAGGATGAGGTTTCACAGCAAACCCATTTAAAGAAAGCTAGGGTTTCTGTCAGAGCTAGATGCAATGCTCCCACGATGAATGATGGATGTCAATGGAGAAAATATGGACAGAAAATAGCAAAAGGAAATCCATGCCCTAGAGCGTACTACCGTTGCACAGTCTCACCGTCTTGCCCTGTGAGAAAACAGGTGCAAAGAAATTCCAAGGACATGTCCATACTAATCACAACCTACGAAGGAAGCCACAACCACCCACTTTCAATGTCAGCCACAGCTATGGCCTCGACCACCTCCGCCGCTGCTTCCATGCTTCAGTCTCACTCATCCACCTCCCAACAAGGCCTCTTCAACTCCGCCACCGCGCCCATCTCCGCCTCCACTAATCTCCAAGGACTAAACTTCAGTGGTACTAGTACTCTCTCTCAAAATTCAAGGCTACCACAGCAACATTTCTACTTCCCCAACAGTTCAGTCTCAACCAACAATTCCCACCCAACTATCACCCTTGATCTCACCGTCCCAACTCCCTCTCATTTCGGAAGATTCCCCTTGGCCTCGGGCAGCAGCTTTTCTTCCAACCCAAGATATCCTTCAACATCTTTCAACTTCTCGTCTTCTTTAGACCACAATAACACATTGCAGTTGCAATCTCCTTGGAACAGCATCAGTCACACAGCCTCTGGATATTTCAATTATGGGAATTATGGGAATAGAATTAACCAAGTTGGATCAGCCCTAAACATCGGAAAGCAACCAATCTTTCAAGAACATAATAAATTGTACCAATCCCACGTACAAAATCAAAACGCCTCTCCTCCTCCTCCTCCCCTTCATCATCCTCAGATGTCAACCAACTCAATAGCCACAGCCACAAAGGCAATCACATTGAACCCGAACTTTCAATCGGCTTTGGCAGCTGCACTTACATCATTTCTAGGCAATGGGGGTACTAGCAGCACTACTGGGATCAGAGAAAACCATAAAAGTGGGACTATTGTAGAAAGTTCTTCTGGGCTGAAATTGAAGCCGAGTGAGTCGTTGACGTCGAATTCAATATCCCATCAAGCCAAAATGGAATTTCGGGAATTAGAAGCCCAACAGGTATTCAGTGCCTACACTTGGCAAAGCTCGCTTCATGCATGCAGAGGCATCACTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

588

Amino Acids

64.14

Weight (kDa)

6.43

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 228 - 286 3e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1649
AccII CGCG 1 cut(s) 965
AciI CCGC 6 cut(s) 569, 897, 900, 957, 963, 975
AclWI GGATC 2 cut(s) 1339, 1577
AcoI YGGCCR 1 cut(s) 566
AcsI RAATTY 4 cut(s) 802, 1030, 1651, 1679
AcuI CTGAAG 2 cut(s) 899, 988
AcyI GRCGYC 1 cut(s) 1646
AfaI GTAC 9 cut(s) 97, 271, 292, 749, 1012, 1018, 1385, 1398, 1552
AfiI CCNNNNNNNGG 1 cut(s) 1722
AflIII ACRYGT 1 cut(s) 813
AhlI ACTAGT 1 cut(s) 1013
AjuI GAANNNNNNNTTGG 2 cut(s) 1177, 1209
Alw26I GTCTC 4 cut(s) 101, 769, 924, 1081
AlwI GGATC 2 cut(s) 1339, 1577
AlwNI CAGNNNCTG 3 cut(s) 217, 506, 1283
Ama87I CYCGRG 2 cut(s) 199, 1159
AoxI GGCC 6 cut(s) 235, 535, 566, 882, 943, 1155
ApeKI GCWGC 9 cut(s) 454, 486, 503, 902, 1164, 1167, 1517, 1520, 1557
ApoI RAATTY 4 cut(s) 802, 1030, 1651, 1679
AspLEI GCGC 1 cut(s) 967
AspS9I GGNCC 1 cut(s) 536
AsuHPI GGTGA 4 cut(s) 92, 759, 1096, 1108
AvaI CYCGRG 2 cut(s) 199, 1159
BauI CACGAG 1 cut(s) 297
BbsI GAAGAC 4 cut(s) 63, 552, 593, 1209
BbvI GCAGC 9 cut(s) 441, 473, 490, 889, 1176, 1179, 1507, 1529, 1569
BccI CCATC 5 cut(s) 71, 389, 680, 977, 1671
BclI TGATCA 3 cut(s) 82, 379, 508
BcoDI GTCTC 4 cut(s) 101, 769, 924, 1081
BcuI ACTAGT 1 cut(s) 1013
BfaI CTAG 9 cut(s) 279, 341, 573, 642, 660, 741, 1014, 1538, 1554
BfmI CTRYAG 2 cut(s) 218, 487
BglI GCCNNNNNGGC 1 cut(s) 1478
BglII AGATCT 1 cut(s) 491
BmcAI AGTACT 2 cut(s) 292, 1018
BmeT110I CYCGRG 2 cut(s) 199, 1159
BmgT120I GGNCC 1 cut(s) 536
BmiI GGNNCC 1 cut(s) 404
BmrI ACTGGG 1 cut(s) 1575
BmsI GCATC 5 cut(s) 213, 235, 265, 653, 1275
BmuI ACTGGG 1 cut(s) 1575
BpiI GAAGAC 4 cut(s) 63, 552, 593, 1209
BpmI CTGGAG 2 cut(s) 497, 552
BpuEI CTTGAG 1 cut(s) 352
BsaAI YACGTR 1 cut(s) 1396
BsaBI GATNNNNATC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 1646
BsaJI CCNNGG 8 cut(s) 102, 114, 355, 807, 991, 1152, 1158, 1256
Bsc4I CCNNNNNNNGG 1 cut(s) 1722
Bse1I ACTGG 4 cut(s) 211, 406, 480, 1570
Bse3DI GCAATG 3 cut(s) 673, 1238, 1549
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 8 cut(s) 102, 114, 355, 807, 991, 1152, 1158, 1256
BseGI GGATG 7 cut(s) 82, 124, 400, 613, 695, 926, 1438
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 1722
BseMI GCAATG 3 cut(s) 673, 1238, 1549
BseMII CTCAG 2 cut(s) 549, 1457
BseNI ACTGG 4 cut(s) 211, 406, 480, 1570
BseRI GAGGAG 4 cut(s) 1407, 1410, 1413, 1416
BseXI GCAGC 9 cut(s) 441, 473, 490, 889, 1176, 1179, 1507, 1529, 1569
BsgI GTGCAG 1 cut(s) 1506
Bsh1236I CGCG 1 cut(s) 965
BshFI GGCC 6 cut(s) 237, 537, 568, 884, 945, 1157
BsiHKCI CYCGRG 2 cut(s) 199, 1159
BslFI GGGAC 2 cut(s) 1106, 1606
BslI CCNNNNNNNGG 1 cut(s) 1722
BsmAI GTCTC 4 cut(s) 101, 769, 924, 1081
BsmFI GGGAC 2 cut(s) 1106, 1606
BsnI GGCC 6 cut(s) 237, 537, 568, 884, 945, 1157
BsoBI CYCGRG 2 cut(s) 199, 1159
Bsp143I GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 6 cut(s) 569, 897, 900, 957, 963, 975
BspANI GGCC 6 cut(s) 237, 537, 568, 884, 945, 1157
BspCNI CTCAG 2 cut(s) 550, 1456
BspFNI CGCG 1 cut(s) 965
BspHI TCATGA 2 cut(s) 19, 85
BspLI GGNNCC 1 cut(s) 404
BspMAI CTGCAG 2 cut(s) 222, 491
BspPI GGATC 2 cut(s) 1339, 1577
BsrDI GCAATG 3 cut(s) 673, 1238, 1549
BsrI ACTGG 4 cut(s) 211, 406, 480, 1570
BssECI CCNNGG 8 cut(s) 102, 114, 355, 807, 991, 1152, 1158, 1256
BssMI GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
BssNI GRCGYC 1 cut(s) 1646
BssSI CACGAG 1 cut(s) 297
BssT1I CCWWGG 7 cut(s) 102, 114, 355, 807, 991, 1152, 1256
Bst2BI CACGAG 1 cut(s) 297
Bst4CI ACNGT 6 cut(s) 67, 755, 763, 771, 1070, 1120
Bst6I CTCTTC 2 cut(s) 84, 953
BstACI GRCGYC 1 cut(s) 1646
BstAPI GCANNNNNTGC 1 cut(s) 1758
BstBAI YACGTR 1 cut(s) 1396
BstC8I GCNNGC 2 cut(s) 1733, 1744
BstDEI CTNAG 4 cut(s) 261, 350, 558, 1443
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 7 cut(s) 82, 124, 400, 613, 695, 926, 1438
BstFNI CGCG 1 cut(s) 965
BstHHI GCGC 1 cut(s) 967
BstKTI GATC 8 cut(s) 85, 382, 494, 511, 553, 1114, 1334, 1572
BstMAI GTCTC 4 cut(s) 101, 769, 924, 1081
BstMBI GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
BstMWI GCNNNNNNNGC 7 cut(s) 470, 534, 1045, 1478, 1517, 1732, 1758
BstNSI RCATGY 2 cut(s) 817, 1746
BstSFI CTRYAG 2 cut(s) 218, 487
BstUI CGCG 1 cut(s) 965
BstV1I GCAGC 9 cut(s) 441, 473, 490, 889, 1176, 1179, 1507, 1529, 1569
BstV2I GAAGAC 4 cut(s) 63, 552, 593, 1209
BstX2I RGATCY 1 cut(s) 491
BstXI CCANNNNNNTGG 2 cut(s) 880, 1589
BstYI RGATCY 1 cut(s) 491
BsuRI GGCC 6 cut(s) 237, 537, 568, 884, 945, 1157
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 7 cut(s) 82, 124, 400, 613, 695, 926, 1438
BtsIMutI CAGTG 3 cut(s) 473, 1012, 1717
Cac8I GCNNGC 2 cut(s) 1733, 1744
CaiI CAGNNNCTG 3 cut(s) 217, 506, 1283
CciI TCATGA 2 cut(s) 19, 85
CfoI GCGC 1 cut(s) 967
Cfr13I GGNCC 1 cut(s) 536
Csp6I GTAC 9 cut(s) 96, 270, 291, 748, 1011, 1017, 1384, 1397, 1551
CviQI GTAC 9 cut(s) 96, 270, 291, 748, 1011, 1017, 1384, 1397, 1551
DdeI CTNAG 4 cut(s) 261, 350, 558, 1443
DpnI GATC 8 cut(s) 84, 381, 493, 510, 552, 1113, 1333, 1571
DpnII GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
DraI TTTAAA 1 cut(s) 633
EaeI YGGCCR 1 cut(s) 566
Eam1104I CTCTTC 2 cut(s) 84, 953
EarI CTCTTC 2 cut(s) 84, 953
EciI GGCGGA 3 cut(s) 886, 946, 964
Eco130I CCWWGG 7 cut(s) 102, 114, 355, 807, 991, 1152, 1256
Eco147I AGGCCT 1 cut(s) 945
Eco32I GATATC 1 cut(s) 1190
Eco57I CTGAAG 2 cut(s) 899, 988
Eco88I CYCGRG 2 cut(s) 199, 1159
EcoRI GAATTC 1 cut(s) 1651
EcoRV GATATC 1 cut(s) 1190
EcoT14I CCWWGG 7 cut(s) 102, 114, 355, 807, 991, 1152, 1256
EcoT22I ATGCAT 2 cut(s) 228, 1744
ErhI CCWWGG 7 cut(s) 102, 114, 355, 807, 991, 1152, 1256
FalI AAGNNNNNCTT 2 cut(s) 1177, 1209
FaqI GGGAC 2 cut(s) 1106, 1606
FbaI TGATCA 3 cut(s) 82, 379, 508
FokI GGATG 7 cut(s) 89, 131, 407, 620, 702, 913, 1425
FspBI CTAG 9 cut(s) 279, 341, 573, 642, 660, 741, 1014, 1538, 1554
GlaI GCGC 1 cut(s) 966
GsuI CTGGAG 2 cut(s) 497, 552
HaeIII GGCC 6 cut(s) 237, 537, 568, 884, 945, 1157
HhaI GCGC 1 cut(s) 967
Hin1I GRCGYC 1 cut(s) 1646
Hin6I GCGC 1 cut(s) 965
HinP1I GCGC 1 cut(s) 965
HincII GTYRAC 3 cut(s) 516, 1452, 1644
HindII GTYRAC 3 cut(s) 516, 1452, 1644
HinfI GANTC 2 cut(s) 1146, 1637
HphI GGTGA 4 cut(s) 92, 759, 1096, 1108
Hpy166II GTNNAC 3 cut(s) 516, 1452, 1644
Hpy188I TCNGA 9 cut(s) 76, 402, 496, 555, 656, 1142, 1349, 1446, 1574
Hpy188III TCNNGA 8 cut(s) 20, 86, 199, 299, 531, 1284, 1367, 1685
Hpy8I GTNNAC 3 cut(s) 516, 1452, 1644
Hpy99I CGWCG 3 cut(s) 440, 595, 1651
HpyAV CCTTC 5 cut(s) 248, 535, 833, 1203, 1442
HpyCH4III ACNGT 6 cut(s) 67, 755, 763, 771, 1070, 1120
HpyCH4IV ACGT 3 cut(s) 581, 1395, 1646
HpyF10VI GCNNNNNNNGC 7 cut(s) 470, 534, 1045, 1478, 1517, 1732, 1758
HpyF3I CTNAG 4 cut(s) 261, 350, 558, 1443
HpySE526I ACGT 3 cut(s) 581, 1395, 1646
Hsp92I GRCGYC 1 cut(s) 1646
HspAI GCGC 1 cut(s) 965
Ksp22I TGATCA 3 cut(s) 82, 379, 508
Kzo9I GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
LmnI GCTCC 3 cut(s) 402, 461, 676
Lsp1109I GCAGC 9 cut(s) 441, 473, 490, 889, 1176, 1179, 1507, 1529, 1569
LweI GCATC 5 cut(s) 213, 235, 265, 653, 1275
MaeI CTAG 9 cut(s) 279, 341, 573, 642, 660, 741, 1014, 1538, 1554
MaeII ACGT 3 cut(s) 581, 1395, 1646
MaeIII GTNAC 1 cut(s) 1271
MalI GATC 8 cut(s) 84, 381, 493, 510, 552, 1113, 1333, 1571
MboI GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
MflI RGATCY 1 cut(s) 491
MlyI GAGTC 1 cut(s) 1646
MmeI TCCRAC 3 cut(s) 380, 1203, 1309
Mph1103I ATGCAT 2 cut(s) 228, 1744
MseI TTAA 2 cut(s) 632, 1320
MslI CAYNNNNRTG 3 cut(s) 231, 878, 1587
MspA1I CMGCKG 3 cut(s) 503, 902, 1520
MvnI CGCG 1 cut(s) 965
MwoI GCNNNNNNNGC 7 cut(s) 470, 534, 1045, 1478, 1517, 1732, 1758
NcoI CCATGG 1 cut(s) 102
NdeII GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
NlaIV GGNNCC 1 cut(s) 404
NmeAIII GCCGAG 1 cut(s) 1656
NmuCI GTSAC 1 cut(s) 1271
NsiI ATGCAT 2 cut(s) 228, 1744
NspI RCATGY 2 cut(s) 817, 1746
PaeI GCATGC 1 cut(s) 1746
PaeR7I CTCGAG 1 cut(s) 199
PagI TCATGA 2 cut(s) 19, 85
PceI AGGCCT 1 cut(s) 945
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 2 cut(s) 587, 1646
PfeI GAWTC 1 cut(s) 1146
PleI GAGTC 1 cut(s) 1645
PpsI GAGTC 1 cut(s) 1645
Ppu21I YACGTR 1 cut(s) 1396
PscI ACATGT 1 cut(s) 813
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 1 cut(s) 536
PstI CTGCAG 2 cut(s) 222, 491
PstNI CAGNNNCTG 3 cut(s) 217, 506, 1283
PsuI RGATCY 1 cut(s) 491
PvuII CAGCTG 2 cut(s) 503, 1520
RsaI GTAC 9 cut(s) 97, 271, 292, 749, 1012, 1018, 1385, 1398, 1552
RsaNI GTAC 9 cut(s) 96, 270, 291, 748, 1011, 1017, 1384, 1397, 1551
RseI CAYNNNNRTG 3 cut(s) 231, 878, 1587
SaqAI TTAA 2 cut(s) 632, 1320
Sau3AI GATC 8 cut(s) 82, 379, 491, 508, 550, 1111, 1331, 1569
Sau96I GGNCC 1 cut(s) 536
ScaI AGTACT 2 cut(s) 292, 1018
SchI GAGTC 1 cut(s) 1646
SfaNI GCATC 5 cut(s) 213, 235, 265, 653, 1275
SfcI CTRYAG 2 cut(s) 218, 487
Sfr274I CTCGAG 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 199
SmiMI CAYNNNNRTG 3 cut(s) 231, 878, 1587
SmlI CTYRAG 2 cut(s) 199, 367
SmoI CTYRAG 2 cut(s) 199, 367
SpeI ACTAGT 1 cut(s) 1013
SphI GCATGC 1 cut(s) 1746
SseBI AGGCCT 1 cut(s) 945
SsiI CCGC 6 cut(s) 569, 897, 900, 957, 963, 975
SspI AATATT 1 cut(s) 322
SspMI CTAG 9 cut(s) 279, 341, 573, 642, 660, 741, 1014, 1538, 1554
StuI AGGCCT 1 cut(s) 945
StyI CCWWGG 7 cut(s) 102, 114, 355, 807, 991, 1152, 1256
TaaI ACNGT 6 cut(s) 67, 755, 763, 771, 1070, 1120
TaiI ACGT 3 cut(s) 584, 1398, 1649
TaqI TCGA 3 cut(s) 200, 887, 1649
TatI WGTACW 2 cut(s) 290, 1016
TauI GCSGC 2 cut(s) 571, 902
TfiI GAWTC 1 cut(s) 1146
Tru1I TTAA 2 cut(s) 632, 1320
Tru9I TTAA 2 cut(s) 632, 1320
TscAI CASTG 3 cut(s) 480, 1012, 1717
TseFI GTSAC 1 cut(s) 1271
TseI GCWGC 9 cut(s) 454, 486, 503, 902, 1164, 1167, 1517, 1520, 1557
Tsp45I GTSAC 1 cut(s) 1271
TspDTI ATGAA 7 cut(s) 102, 135, 221, 264, 695, 1424, 1727
TspRI CASTG 3 cut(s) 480, 1012, 1717
XapI RAATTY 4 cut(s) 802, 1030, 1651, 1679
XceI RCATGY 2 cut(s) 817, 1746
XhoI CTCGAG 1 cut(s) 199
XspI CTAG 9 cut(s) 279, 341, 573, 642, 660, 741, 1014, 1538, 1554
ZraI GACGTC 1 cut(s) 1647
ZrmI AGTACT 2 cut(s) 292, 1018
Zsp2I ATGCAT 2 cut(s) 228, 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.