Rroxscaffold_5G00381260
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008989
Physical Location & Seq
Reverse (-)
61286191 .. 61295381
9191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00381260.1

Sequence Viewer

Length: 1899 bp
ATGATTGGTATTTGGGGAAGGAGAGGGTTCCGAAATGAGAGACAGACAGAGAGAAAAGCAAAGAGAGAGAGTGTGATAAGGGTGAGTGCGAGAGAGAAAGAGCAGCTCGGGAGCCGGGTTGTAGTGGAGCGAGAAAAGAAAACAAAGAGGCGTTCAAAATCAATGGAGATTGAAAAGTCTTTGCTGGGAAATTCAGTTATGGAAGAGAACAGATTGAAATCTGATGGTGATGATTCAGAAGGCACTTCCAAAGAGATAAACAAGGGAGGAGATGTTGAAGAAGTAGCTGAAGGAAAATACAATTTGAAGCTGCAGCCTTCACCTACACAAAAGGATAGAGCTCAGAGCAAGCACAAACAGGAAGCAGATGATGAACTGGAATTAGCTAAAGCTGAAATGGGGGAGGTTAGAGAAGAAAATAAAAGGCTAAAGGTGTTATTAGCGCAAATCGTGAAGGATTACCAGGAACTTCAGATGCATTTTCTTGATGTTATTCAAAAAGAAGAAACAAATAAGAAATCTATGGATACCAGTAGTACAGCTGATCATCAAGTAACTGTTTCGGCTGAAGCTGATGAACTTGTGTCCCTTAGCCTTGGGAGAACCTCGAGTAGTACTAGTACTCATGATCATCAGCTCAGAAAGGATCATCATGACATGATGAAAAAGATCAGCAGAGATGACGATTACGGGGTAATGAATGAAGCTGGACTTGCATTGGAATTGGGATGCAGATCATTTGAGCCAGCTGCTGCTGATCATGAAACTATGAAGGTTAATTCAAGCTCCGAAATTAATAGTTCAGCCGCAGATCCAAAGGAAGACGAAGTAACTAAGATATGGCCGCCTAGTAAAATGTTGAAGACAAGAGATGATGATGTTTCACAACAGCAGACCCATTTGAAGAAAGCTAGGGTTTCTGTCCGAGCTAGATGCGATGCTCCAACAGTGCAAAGATGTGCGGAAGACATGTCTATATTGATCACAACCTATGAGGGTAACCACAACCATCCACTTCCCATGTCAGCCACGGCCATGGCCTCCACCACCTCCGCTGCGGCATCCATGCTTCAGTCTCACTCATCCACCTCGCAACAAGGCCTTGTCAACTCAACCACCGCCCCCATCTCCACCTCCACTTGTAATCTCCATGGCCATGGACCAAACTTCACCAATAGTCTCTCCCAGAATTCATCGAGACTACTACCACAGTCACAGTTCTATTTCCCCAACAGTTCAATCTCAACCAACAATTCCCACCCAACCGTCACCCTTGATCTCACTGCTCCATCTCCCTCCCATTTCGGACTAAGATTTCCTTCAGGGTTACCGAGATATTCCTCGAGTACAAGCCTCAACTTTTCGTCATCGTCGTCCTCTTCTTTCGACCACAACACATTACTACAAGCACCAAATTGGATTAACAATCCCGCTGCTGCAGGCTATTTCAACTATGGGACGGTACTATCTCATCAGAATAGGATGAACCAAGCTGTTGGTGGGTCTTCTCTTAACAACGGAAAGCAGCCATTTCAAGAACCTAATTTCTACCAATCTTACATACAAAATTCTCAGAAAGCAGCTCCTGCTCCACCTCAGCATCATCTGTTTACAGAGACATTAGCGACTGCAACAAAAGCAATCACATCAAACCCTAAGTTTCAATCAGCTTTAGCAGCGGCACTCACGTCATTTGTTGGTGCTAATGGGGGTACTAGCGGGGCGGTCAGAGAAACTAGTCACATCGTTAATGGCAGTAGTAGTACTGAAAGTTCTGGGCTGAAATTGAAGTGGGGTGAGTCCTTGACGCCGATTAATCCAATCTACCCATCAACCCAGAAAGGAATCAAGTGCGCATCATCAGGCTACTTGAACAAATCATCGTCGCCATTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

632

Amino Acids

69.15

Weight (kDa)

8.1

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1855
AclWI GGATC 2 cut(s) 654, 806
AcoI YGGCCR 3 cut(s) 842, 1032, 1153
AcsI RAATTY 3 cut(s) 190, 1189, 1567
AcuI CTGAAG 5 cut(s) 309, 455, 588, 1055, 1305
AcyI GRCGYC 1 cut(s) 1808
AfaI GTAC 7 cut(s) 538, 616, 622, 1348, 1464, 1714, 1765
AflIII ACRYGT 1 cut(s) 969
AhlI ACTAGT 2 cut(s) 617, 1736
AjiI CACGTC 1 cut(s) 1689
AjnI CCWGG 1 cut(s) 462
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 5 cut(s) 34, 1080, 1184, 1192, 1610
AlwI GGATC 2 cut(s) 654, 806
AlwNI CAGNNNCTG 2 cut(s) 752, 1586
Ama87I CYCGRG 3 cut(s) 107, 607, 1342
AoxI GGCC 5 cut(s) 842, 1032, 1038, 1099, 1153
ApoI RAATTY 3 cut(s) 190, 1189, 1567
AseI ATTAAT 2 cut(s) 795, 1815
AspLEI GCGC 2 cut(s) 445, 1856
AspS9I GGNCC 1 cut(s) 1160
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 6 cut(s) 94, 239, 312, 1162, 1261, 1808
AvaI CYCGRG 3 cut(s) 107, 607, 1342
AvaII GGWCC 1 cut(s) 1160
BalI TGGCCA 1 cut(s) 1155
BanII GRGCYC 1 cut(s) 343
BbsI GAAGAC 4 cut(s) 828, 869, 972, 1497
Bbv12I GWGCWC 1 cut(s) 343
BbvCI CCTCAGC 1 cut(s) 1596
BccI CCATC 5 cut(s) 218, 1017, 1133, 1297, 1837
BceAI ACGGC 1 cut(s) 1047
BcgI CGANNNNNNTGC 2 cut(s) 915, 949
BciT130I CCWGG 1 cut(s) 464
BciVI GTATCC 1 cut(s) 520
BclI TGATCA 4 cut(s) 544, 628, 757, 981
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 5 cut(s) 34, 1080, 1184, 1192, 1610
BcuI ACTAGT 2 cut(s) 617, 1736
BfaI CTAG 6 cut(s) 618, 849, 912, 930, 1716, 1737
BfmI CTRYAG 2 cut(s) 311, 1437
BfuI GTATCC 1 cut(s) 520
BmcAI AGTACT 3 cut(s) 616, 622, 1765
Bme1390I CCNGG 2 cut(s) 116, 464
Bme18I GGWCC 1 cut(s) 1160
BmeT110I CYCGRG 3 cut(s) 107, 607, 1342
BmgBI CACGTC 1 cut(s) 1689
BmgT120I GGNCC 1 cut(s) 1160
BmiI GGNNCC 2 cut(s) 29, 113
BmrFI CCNGG 2 cut(s) 116, 464
BmsI GCATC 7 cut(s) 465, 719, 923, 928, 1070, 1609, 1865
BpiI GAAGAC 4 cut(s) 828, 869, 972, 1497
Bpu10I CCTNAGC 2 cut(s) 590, 1596
BpuMI CCSGG 1 cut(s) 116
BsaBI GATNNNNATC 2 cut(s) 217, 733
BsaHI GRCGYC 1 cut(s) 1808
BsaJI CCNNGG 5 cut(s) 595, 1029, 1035, 1150, 1156
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse1I ACTGG 2 cut(s) 381, 531
Bse8I GATNNNNATC 2 cut(s) 217, 733
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 5 cut(s) 595, 1029, 1035, 1150, 1156
BseGI GGATG 5 cut(s) 734, 1009, 1061, 1082, 1488
BseJI GATNNNNATC 2 cut(s) 217, 733
BseMII CTCAG 4 cut(s) 356, 652, 1586, 1610
BseNI ACTGG 2 cut(s) 381, 531
BseRI GAGGAG 1 cut(s) 282
BseYI CCCAGC 1 cut(s) 184
BshFI GGCC 5 cut(s) 844, 1034, 1040, 1101, 1155
BsiHKAI GWGCWC 1 cut(s) 343
BsiHKCI CYCGRG 3 cut(s) 107, 607, 1342
BsiSI CCGG 1 cut(s) 115
BslFI GGGAC 2 cut(s) 571, 1471
BsmAI GTCTC 5 cut(s) 34, 1080, 1184, 1192, 1610
BsmFI GGGAC 2 cut(s) 571, 1471
BsnI GGCC 5 cut(s) 844, 1034, 1040, 1101, 1155
BsoBI CYCGRG 3 cut(s) 107, 607, 1342
Bsp1286I GDGCHC 1 cut(s) 343
Bsp143I GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
Bsp19I CCATGG 3 cut(s) 1035, 1150, 1156
BspANI GGCC 5 cut(s) 844, 1034, 1040, 1101, 1155
BspCNI CTCAG 4 cut(s) 355, 651, 1585, 1609
BspHI TCATGA 3 cut(s) 625, 652, 760
BspLI GGNNCC 2 cut(s) 29, 113
BspMAI CTGCAG 2 cut(s) 315, 1441
BspPI GGATC 2 cut(s) 654, 806
BsrI ACTGG 2 cut(s) 381, 531
BssECI CCNNGG 5 cut(s) 595, 1029, 1035, 1150, 1156
BssMI GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
BssNI GRCGYC 1 cut(s) 1808
BssT1I CCWWGG 4 cut(s) 595, 1035, 1150, 1156
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 7 cut(s) 559, 949, 1212, 1218, 1235, 1267, 1462
Bst6I CTCTTC 2 cut(s) 198, 1384
BstACI GRCGYC 1 cut(s) 1808
BstAPI GCANNNNNTGC 1 cut(s) 1586
BstC8I GCNNGC 3 cut(s) 350, 747, 1441
BstDEI CTNAG 8 cut(s) 342, 590, 638, 834, 1310, 1572, 1596, 1656
BstDSI CCRYGG 4 cut(s) 1029, 1035, 1150, 1156
BstEII GGTNACC 2 cut(s) 998, 1326
BstF5I GGATG 5 cut(s) 734, 1009, 1061, 1082, 1488
BstHHI GCGC 2 cut(s) 445, 1856
BstKTI GATC 9 cut(s) 547, 631, 649, 672, 737, 760, 814, 984, 1279
BstMAI GTCTC 5 cut(s) 34, 1080, 1184, 1192, 1610
BstMBI GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
BstMWI GCNNNNNNNGC 4 cut(s) 713, 1586, 1637, 1676
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 1 cut(s) 973
BstPI GGTNACC 2 cut(s) 998, 1326
BstSCI CCNGG 2 cut(s) 114, 462
BstSFI CTRYAG 2 cut(s) 311, 1437
BstV2I GAAGAC 4 cut(s) 828, 869, 972, 1497
BstX2I RGATCY 1 cut(s) 811
BstXI CCANNNNNNTGG 3 cut(s) 1036, 1157, 1496
BstYI RGATCY 1 cut(s) 811
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 5 cut(s) 844, 1034, 1040, 1101, 1155
BtgI CCRYGG 4 cut(s) 1029, 1035, 1150, 1156
BtgZI GCGATG 1 cut(s) 951
BtrI CACGTC 1 cut(s) 1689
BtsCI GGATG 5 cut(s) 734, 1009, 1061, 1082, 1488
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 2 cut(s) 954, 1281
Cac8I GCNNGC 3 cut(s) 350, 747, 1441
CaiI CAGNNNCTG 2 cut(s) 752, 1586
CciI TCATGA 3 cut(s) 625, 652, 760
CfoI GCGC 2 cut(s) 445, 1856
Cfr13I GGNCC 1 cut(s) 1160
CseI GACGC 1 cut(s) 1816
Csp6I GTAC 7 cut(s) 537, 615, 621, 1347, 1463, 1713, 1764
CviQI GTAC 7 cut(s) 537, 615, 621, 1347, 1463, 1713, 1764
DdeI CTNAG 8 cut(s) 342, 590, 638, 834, 1310, 1572, 1596, 1656
DpnI GATC 9 cut(s) 546, 630, 648, 671, 736, 759, 813, 983, 1278
DpnII GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
EaeI YGGCCR 3 cut(s) 842, 1032, 1153
Eam1104I CTCTTC 2 cut(s) 198, 1384
EarI CTCTTC 2 cut(s) 198, 1384
Ecl136II GAGCTC 1 cut(s) 341
Eco130I CCWWGG 4 cut(s) 595, 1035, 1150, 1156
Eco147I AGGCCT 1 cut(s) 1101
Eco24I GRGCYC 1 cut(s) 343
Eco47I GGWCC 1 cut(s) 1160
Eco53kI GAGCTC 1 cut(s) 341
Eco57I CTGAAG 5 cut(s) 309, 455, 588, 1055, 1305
Eco88I CYCGRG 3 cut(s) 107, 607, 1342
Eco91I GGTNACC 2 cut(s) 998, 1326
EcoICRI GAGCTC 1 cut(s) 341
EcoO65I GGTNACC 2 cut(s) 998, 1326
EcoRI GAATTC 1 cut(s) 1189
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 4 cut(s) 595, 1035, 1150, 1156
EcoT22I ATGCAT 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 4 cut(s) 595, 1035, 1150, 1156
FalI AAGNNNNNCTT 4 cut(s) 696, 728, 1303, 1335
FaqI GGGAC 2 cut(s) 571, 1471
FauI CCCGC 2 cut(s) 1438, 1712
FbaI TGATCA 4 cut(s) 544, 628, 757, 981
FokI GGATG 5 cut(s) 741, 996, 1048, 1069, 1495
FriOI GRGCYC 1 cut(s) 343
FspAI RTGCGCAY 1 cut(s) 1855
FspBI CTAG 6 cut(s) 618, 849, 912, 930, 1716, 1737
FspI TGCGCA 1 cut(s) 1855
GlaI GCGC 2 cut(s) 444, 1855
GsaI CCCAGC 1 cut(s) 188
HaeIII GGCC 5 cut(s) 844, 1034, 1040, 1101, 1155
HapII CCGG 1 cut(s) 115
HgaI GACGC 1 cut(s) 1816
HhaI GCGC 2 cut(s) 445, 1856
Hin1I GRCGYC 1 cut(s) 1808
Hin6I GCGC 2 cut(s) 443, 1854
HinP1I GCGC 2 cut(s) 443, 1854
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HinfI GANTC 3 cut(s) 233, 1799, 1845
HpaII CCGG 1 cut(s) 115
HphI GGTGA 6 cut(s) 94, 239, 312, 1162, 1261, 1808
Hpy166II GTNNAC 2 cut(s) 1108, 1611
Hpy188III TCNNGA 8 cut(s) 109, 451, 485, 626, 653, 761, 1197, 1535
Hpy8I GTNNAC 2 cut(s) 1108, 1611
Hpy99I CGWCG 2 cut(s) 1375, 1888
HpyAV CCTTC 7 cut(s) 12, 233, 284, 327, 448, 766, 1329
HpyCH4III ACNGT 7 cut(s) 559, 949, 1212, 1218, 1235, 1267, 1462
HpyCH4IV ACGT 1 cut(s) 1688
HpyCH4V TGCA 7 cut(s) 313, 478, 716, 732, 952, 1439, 1631
HpyF10VI GCNNNNNNNGC 4 cut(s) 713, 1586, 1637, 1676
HpyF3I CTNAG 8 cut(s) 342, 590, 638, 834, 1310, 1572, 1596, 1656
HpySE526I ACGT 1 cut(s) 1688
Hsp92I GRCGYC 1 cut(s) 1808
HspAI GCGC 2 cut(s) 443, 1854
Ksp22I TGATCA 4 cut(s) 544, 628, 757, 981
Kzo9I GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
LmnI GCTCC 7 cut(s) 111, 127, 791, 946, 1291, 1588, 1594
LweI GCATC 7 cut(s) 465, 719, 923, 928, 1070, 1609, 1865
MaeI CTAG 6 cut(s) 618, 849, 912, 930, 1716, 1737
MaeII ACGT 1 cut(s) 1688
MaeIII GTNAC 7 cut(s) 553, 829, 998, 1212, 1267, 1326, 1739
MalI GATC 9 cut(s) 546, 630, 648, 671, 736, 759, 813, 983, 1278
MboI GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 1 cut(s) 343
MlsI TGGCCA 1 cut(s) 1155
MluNI TGGCCA 1 cut(s) 1155
MlyI GAGTC 1 cut(s) 1808
MmeI TCCRAC 1 cut(s) 968
Mox20I TGGCCA 1 cut(s) 1155
Mph1103I ATGCAT 1 cut(s) 480
MscI TGGCCA 1 cut(s) 1155
MseI TTAA 6 cut(s) 777, 795, 1422, 1512, 1749, 1815
MslI CAYNNNNRTG 2 cut(s) 1034, 1155
Msp20I TGGCCA 1 cut(s) 1155
MspA1I CMGCKG 5 cut(s) 542, 749, 1055, 1433, 1679
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 116, 464
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 713, 1586, 1637, 1676
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 3 cut(s) 1035, 1150, 1156
NdeII GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
NlaIV GGNNCC 2 cut(s) 29, 113
NmuCI GTSAC 3 cut(s) 1212, 1267, 1739
NsbI TGCGCA 1 cut(s) 1855
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 2 cut(s) 607, 1342
PagI TCATGA 3 cut(s) 625, 652, 760
PceI AGGCCT 1 cut(s) 1101
PciI ACATGT 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 1370
PfeI GAWTC 2 cut(s) 233, 1845
PleI GAGTC 1 cut(s) 1807
PpsI GAGTC 1 cut(s) 1807
PscI ACATGT 1 cut(s) 969
PshBI ATTAAT 2 cut(s) 795, 1815
Psp124BI GAGCTC 1 cut(s) 343
Psp6I CCWGG 1 cut(s) 462
PspEI GGTNACC 2 cut(s) 998, 1326
PspFI CCCAGC 1 cut(s) 184
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 29, 113
PspPI GGNCC 1 cut(s) 1160
PspXI VCTCGAGB 2 cut(s) 607, 1342
PsrI GAACNNNNNNTAC 2 cut(s) 1756, 1788
PstI CTGCAG 2 cut(s) 315, 1441
PstNI CAGNNNCTG 2 cut(s) 752, 1586
PsuI RGATCY 1 cut(s) 811
PvuII CAGCTG 2 cut(s) 542, 749
RsaI GTAC 7 cut(s) 538, 616, 622, 1348, 1464, 1714, 1765
RsaNI GTAC 7 cut(s) 537, 615, 621, 1347, 1463, 1713, 1764
RseI CAYNNNNRTG 2 cut(s) 1034, 1155
SacI GAGCTC 1 cut(s) 343
SaqAI TTAA 6 cut(s) 777, 795, 1422, 1512, 1749, 1815
Sau3AI GATC 9 cut(s) 544, 628, 646, 669, 734, 757, 811, 981, 1276
Sau96I GGNCC 1 cut(s) 1160
ScaI AGTACT 3 cut(s) 616, 622, 1765
SchI GAGTC 1 cut(s) 1808
ScrFI CCNGG 2 cut(s) 116, 464
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 7 cut(s) 465, 719, 923, 928, 1070, 1609, 1865
SfcI CTRYAG 2 cut(s) 311, 1437
Sfr274I CTCGAG 2 cut(s) 607, 1342
SinI GGWCC 1 cut(s) 1160
SlaI CTCGAG 2 cut(s) 607, 1342
SmiMI CAYNNNNRTG 2 cut(s) 1034, 1155
SmlI CTYRAG 2 cut(s) 607, 1342
SmoI CTYRAG 2 cut(s) 607, 1342
SpeI ACTAGT 2 cut(s) 617, 1736
SseBI AGGCCT 1 cut(s) 1101
SspMI CTAG 6 cut(s) 618, 849, 912, 930, 1716, 1737
SstI GAGCTC 1 cut(s) 343
StuI AGGCCT 1 cut(s) 1101
StyD4I CCNGG 2 cut(s) 114, 462
StyI CCWWGG 4 cut(s) 595, 1035, 1150, 1156
TaaI ACNGT 7 cut(s) 559, 949, 1212, 1218, 1235, 1267, 1462
TaiI ACGT 1 cut(s) 1691
TaqI TCGA 4 cut(s) 608, 1196, 1343, 1386
TatI WGTACW 5 cut(s) 536, 614, 620, 1346, 1763
TauI GCSGC 4 cut(s) 809, 847, 1061, 1682
TfiI GAWTC 2 cut(s) 233, 1845
Tru1I TTAA 6 cut(s) 777, 795, 1422, 1512, 1749, 1815
Tru9I TTAA 6 cut(s) 777, 795, 1422, 1512, 1749, 1815
TscAI CASTG 2 cut(s) 954, 1288
TseFI GTSAC 3 cut(s) 1212, 1267, 1739
Tsp45I GTSAC 3 cut(s) 1212, 1267, 1739
TspDTI ATGAA 9 cut(s) 387, 591, 677, 713, 717, 777, 785, 1182, 1499
TspGWI ACGGA 1 cut(s) 1533
TspRI CASTG 2 cut(s) 954, 1288
VpaK11BI GGWCC 1 cut(s) 1160
VspI ATTAAT 2 cut(s) 795, 1815
XapI RAATTY 3 cut(s) 190, 1189, 1567
XceI RCATGY 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1496
XhoI CTCGAG 2 cut(s) 607, 1342
XspI CTAG 6 cut(s) 618, 849, 912, 930, 1716, 1737
ZrmI AGTACT 3 cut(s) 616, 622, 1765
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.