Rw4G032290
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008989
Physical Location & Seq
Reverse (-)
59422776 .. 59425865
3090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032290.1

Sequence Viewer

Length: 1785 bp
ATGCAATTAGCAAAGAGGCGTTCAAAATCAATGGAGATGGAAAAGTCTTTGCTGGGAAATTCAGTTATGGACGAGAACAGAATGAAATCTGATGGTGATGATTCAGAAGGCACTTCCAAAGATATAAACAAGGAAGCAGATGATGAACTGGAATTAGCTAAAGCTGAAATGGGGGAGGTTAGAGAAGAAAATAAAAGGCTAAAGGTGTTATTATCGCAAATCGTGAAGGATTACCAGGAACTTCGGATGCATTTTCTTGATGTTATTCAAAAAGAAGAAACAAATAAGAAATCTATGGATACCAGTAGTACAGCTGATCATCAAGGAACTGTTTCGGCTGAAGCTGATGAACTTGTGTCCCTTAGCCTTGGGAGAACCTCGAGTAGTACTAGTACTCATGATCATCAGCTCAGAAAGGATCATCATGAGATGATGAAAAAGACCAGCAGAGATGACGATTACGGGGTAATGAATGAAGCTGGACTTGCATTGGAATTGGGATGCAGGTCATTTGAGCCAGCTGCTGCTGATCATGAAACTATGAAGGTTAATTCAAGCTCCGAAATTAATAGTTCAGCCGCAGATCCAAAGGAAGACGAAGTAACTAAGATATGGCCGCCTGGTAAAATGTTGAAGACAAGAGATGATGATGTTTCACAACAGCAGACCCATTTGAAGAAAGCTAGGGTTTCTGTCCGAGCTAGATGCGATGCTCCAACATTGAATGATGGATGTCAATGGAGAAAATATGGACAGAAAATAGCAAAAGGAAATCCATGCCCTCGAGCATACTACCGTTGCACAGTTTCACCGTCGTGCCCTGTGAGAAAACAAGTGCAAAGATGTGCGGAAGACATGTCGATATTGATCACAACCTATGAGGGTAACCACAACCATCCACTTCCCATGTCAGCCACGGCCATGGCCTCCACCACCTCCGCCGCGGCATCCATGCTTCAGTCTCACTCATCCACCTCGCAACAAGGCCTAGTCAACTCAACCACCGCCCCCATCTCCACCTCCACTAGTAACCTCCATGGCCATGGACCAAACTTCACCAATAGTCTCTCCCAGAATTCATCGAGACTACTACCACAGTCACAGTTCTATTTCCCCAACAGTTCAATCTCAACCAACAATTCCCACCCAACCATCACCCTTGATCTCACTGCTCCATCTCCCTCCCATTTCGGACTAAGATTTCCTTCAGGGTTGCCGAGATATTCCTCGAGTACAAGCCTCAACTTTTCGTCATCGTCTTCCTCTTCTTTCGACCACAACACATTACTACAAACACCAAATTGGATTAACAATCCCGCTGCTGCAGGCTATTTCAACTATGGGACGGTACTATCTCATCAGAATAGGATGAACCAAGCTGTTGGTGGGTCTTCTCTTAACAACGGAAAGCAGCCATTTCAAGAACCTAATTTCTACCAATCTTACATACAAAATTCTCAGAAAGCAGCTCCTGCTCCACCTCAGCATCATCTGTTTACAGAGACATTAGCCACTGCAACAAAGGCAATCACATCAAACCCTAAGTTTCAATCAGCTTTAGCAGCGGCACTCACGTCATTTGTTGGTGCTAATGGGGGTACTAGCGGGGCGGTCAGAGAAACTAGTCACATCGTTAATGGCAGTAGTAGTACTGAAAGTTCTGGGCTGAAATTGAAGTGGGGTGAGTCCTTGACGCCGATTAATCCAGTCTACCCATCAACCCAGAAAGGAATCGGGTGCGCATCATCAGGCTACTTGAACAAATCATCGTCACCATTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

594

Amino Acids

64.6

Weight (kDa)

7.32

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 242 - 300 1.7e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1741
Acc36I ACCTGC 1 cut(s) 495
AccI GTMKAC 1 cut(s) 1710
AccII CGCG 1 cut(s) 944
AclWI GGATC 2 cut(s) 426, 578
AcoI YGGCCR 3 cut(s) 614, 918, 1039
AcsI RAATTY 3 cut(s) 58, 1075, 1453
AcuI CTGAAG 3 cut(s) 360, 941, 1191
AcyI GRCGYC 1 cut(s) 1694
AfaI GTAC 7 cut(s) 310, 388, 394, 1234, 1350, 1600, 1651
AflIII ACRYGT 1 cut(s) 855
AhlI ACTAGT 3 cut(s) 389, 1025, 1622
AjiI CACGTC 1 cut(s) 1575
AjnI CCWGG 2 cut(s) 234, 619
AleI CACNNNNGTG 1 cut(s) 814
Alw26I GTCTC 4 cut(s) 966, 1070, 1078, 1496
AlwI GGATC 2 cut(s) 426, 578
AlwNI CAGNNNCTG 2 cut(s) 524, 1472
Ama87I CYCGRG 3 cut(s) 379, 783, 1228
AoxI GGCC 5 cut(s) 614, 918, 924, 985, 1039
ApeKI GCWGC 7 cut(s) 521, 524, 1319, 1322, 1411, 1466, 1562
ApoI RAATTY 3 cut(s) 58, 1075, 1453
AseI ATTAAT 2 cut(s) 567, 1701
Asp700I GAANNNNTTC 1 cut(s) 331
AspLEI GCGC 1 cut(s) 1742
AspS9I GGNCC 1 cut(s) 1046
AsuHPI GGTGA 6 cut(s) 107, 801, 1048, 1147, 1694, 1764
AvaI CYCGRG 3 cut(s) 379, 783, 1228
AvaII GGWCC 1 cut(s) 1046
BaeGI GKGCMC 1 cut(s) 821
BalI TGGCCA 1 cut(s) 1041
BbsI GAAGAC 5 cut(s) 600, 641, 858, 1251, 1383
BbvCI CCTCAGC 1 cut(s) 1482
BbvI GCAGC 7 cut(s) 508, 511, 1306, 1309, 1423, 1478, 1574
BccI CCATC 8 cut(s) 31, 86, 722, 903, 1019, 1160, 1183, 1723
BceAI ACGGC 1 cut(s) 933
BcgI CGANNNNNNTGC 2 cut(s) 687, 721
BciT130I CCWGG 2 cut(s) 236, 621
BciVI GTATCC 1 cut(s) 292
BclI TGATCA 4 cut(s) 316, 400, 529, 867
BcoDI GTCTC 4 cut(s) 966, 1070, 1078, 1496
BcuI ACTAGT 3 cut(s) 389, 1025, 1622
BfaI CTAG 7 cut(s) 390, 684, 702, 989, 1026, 1602, 1623
BfmI CTRYAG 1 cut(s) 1323
BfuAI ACCTGC 1 cut(s) 495
BfuI GTATCC 1 cut(s) 292
BmcAI AGTACT 3 cut(s) 388, 394, 1651
Bme1390I CCNGG 2 cut(s) 236, 621
Bme18I GGWCC 1 cut(s) 1046
BmeT110I CYCGRG 3 cut(s) 379, 783, 1228
BmgBI CACGTC 1 cut(s) 1575
BmgT120I GGNCC 1 cut(s) 1046
BmrFI CCNGG 2 cut(s) 236, 621
BmsI GCATC 7 cut(s) 237, 491, 695, 700, 956, 1495, 1751
BpiI GAAGAC 5 cut(s) 600, 641, 858, 1251, 1383
Bpu10I CCTNAGC 2 cut(s) 362, 1482
BsaBI GATNNNNATC 1 cut(s) 866
BsaHI GRCGYC 1 cut(s) 1694
BsaJI CCNNGG 6 cut(s) 367, 915, 921, 942, 1036, 1042
Bse1I ACTGG 3 cut(s) 153, 303, 1706
Bse8I GATNNNNATC 1 cut(s) 866
BseBI CCWGG 2 cut(s) 236, 621
BseDI CCNNGG 6 cut(s) 367, 915, 921, 942, 1036, 1042
BseGI GGATG 7 cut(s) 252, 506, 737, 895, 947, 968, 1374
BseJI GATNNNNATC 1 cut(s) 866
BseMII CTCAG 3 cut(s) 424, 1472, 1496
BseNI ACTGG 3 cut(s) 153, 303, 1706
BseSI GKGCMC 1 cut(s) 821
BseXI GCAGC 7 cut(s) 508, 511, 1306, 1309, 1423, 1478, 1574
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 5 cut(s) 616, 920, 926, 987, 1041
BsiHKCI CYCGRG 3 cut(s) 379, 783, 1228
BslFI GGGAC 2 cut(s) 343, 1357
BsmAI GTCTC 4 cut(s) 966, 1070, 1078, 1496
BsmFI GGGAC 2 cut(s) 343, 1357
BsnI GGCC 5 cut(s) 616, 920, 926, 987, 1041
BsoBI CYCGRG 3 cut(s) 379, 783, 1228
Bsp1286I GDGCHC 1 cut(s) 821
Bsp143I GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
Bsp19I CCATGG 3 cut(s) 921, 1036, 1042
BspANI GGCC 5 cut(s) 616, 920, 926, 987, 1041
BspCNI CTCAG 3 cut(s) 423, 1471, 1495
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 3 cut(s) 397, 424, 532
BspMAI CTGCAG 1 cut(s) 1327
BspMI ACCTGC 1 cut(s) 495
BspPI GGATC 2 cut(s) 426, 578
BsrI ACTGG 3 cut(s) 153, 303, 1706
BssECI CCNNGG 6 cut(s) 367, 915, 921, 942, 1036, 1042
BssMI GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
BssNI GRCGYC 1 cut(s) 1694
BssT1I CCWWGG 4 cut(s) 367, 921, 1036, 1042
Bst2UI CCWGG 2 cut(s) 236, 621
Bst4CI ACNGT 8 cut(s) 331, 797, 805, 813, 1098, 1104, 1121, 1348
Bst6I CTCTTC 1 cut(s) 1270
BstACI GRCGYC 1 cut(s) 1694
BstAPI GCANNNNNTGC 1 cut(s) 1472
BstC8I GCNNGC 2 cut(s) 519, 1327
BstDEI CTNAG 7 cut(s) 362, 410, 606, 1196, 1458, 1482, 1542
BstDSI CCRYGG 5 cut(s) 915, 921, 942, 1036, 1042
BstEII GGTNACC 1 cut(s) 884
BstF5I GGATG 7 cut(s) 252, 506, 737, 895, 947, 968, 1374
BstFNI CGCG 1 cut(s) 944
BstHHI GCGC 1 cut(s) 1742
BstKTI GATC 7 cut(s) 319, 403, 421, 532, 586, 870, 1165
BstMAI GTCTC 4 cut(s) 966, 1070, 1078, 1496
BstMBI GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
BstMWI GCNNNNNNNGC 4 cut(s) 485, 1472, 1523, 1562
BstNI CCWGG 2 cut(s) 236, 621
BstNSI RCATGY 1 cut(s) 859
BstPI GGTNACC 1 cut(s) 884
BstSCI CCNGG 2 cut(s) 234, 619
BstSFI CTRYAG 1 cut(s) 1323
BstSLI GKGCMC 1 cut(s) 821
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 7 cut(s) 508, 511, 1306, 1309, 1423, 1478, 1574
BstV2I GAAGAC 5 cut(s) 600, 641, 858, 1251, 1383
BstX2I RGATCY 1 cut(s) 583
BstXI CCANNNNNNTGG 3 cut(s) 922, 1043, 1382
BstYI RGATCY 1 cut(s) 583
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 5 cut(s) 616, 920, 926, 987, 1041
BtgI CCRYGG 5 cut(s) 915, 921, 942, 1036, 1042
BtgZI GCGATG 1 cut(s) 723
BtrI CACGTC 1 cut(s) 1575
BtsCI GGATG 7 cut(s) 252, 506, 737, 895, 947, 968, 1374
BtsI GCAGTG 2 cut(s) 1167, 1512
BtsIMutI CAGTG 2 cut(s) 1167, 1512
BveI ACCTGC 1 cut(s) 495
Cac8I GCNNGC 2 cut(s) 519, 1327
CaiI CAGNNNCTG 2 cut(s) 524, 1472
CciI TCATGA 3 cut(s) 397, 424, 532
CfoI GCGC 1 cut(s) 1742
Cfr13I GGNCC 1 cut(s) 1046
Cfr42I CCGCGG 1 cut(s) 945
CseI GACGC 1 cut(s) 1702
Csp6I GTAC 7 cut(s) 309, 387, 393, 1233, 1349, 1599, 1650
CviQI GTAC 7 cut(s) 309, 387, 393, 1233, 1349, 1599, 1650
DdeI CTNAG 7 cut(s) 362, 410, 606, 1196, 1458, 1482, 1542
DpnI GATC 7 cut(s) 318, 402, 420, 531, 585, 869, 1164
DpnII GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
EaeI YGGCCR 3 cut(s) 614, 918, 1039
Eam1104I CTCTTC 1 cut(s) 1270
EarI CTCTTC 1 cut(s) 1270
EciI GGCGGA 1 cut(s) 928
Eco130I CCWWGG 4 cut(s) 367, 921, 1036, 1042
Eco147I AGGCCT 1 cut(s) 987
Eco47I GGWCC 1 cut(s) 1046
Eco57I CTGAAG 3 cut(s) 360, 941, 1191
Eco88I CYCGRG 3 cut(s) 379, 783, 1228
Eco91I GGTNACC 1 cut(s) 884
EcoO65I GGTNACC 1 cut(s) 884
EcoRI GAATTC 1 cut(s) 1075
EcoRII CCWGG 2 cut(s) 234, 619
EcoT14I CCWWGG 4 cut(s) 367, 921, 1036, 1042
EcoT22I ATGCAT 1 cut(s) 252
ErhI CCWWGG 4 cut(s) 367, 921, 1036, 1042
FalI AAGNNNNNCTT 4 cut(s) 468, 500, 1189, 1221
FaqI GGGAC 2 cut(s) 343, 1357
FauI CCCGC 2 cut(s) 1324, 1598
FbaI TGATCA 4 cut(s) 316, 400, 529, 867
FblI GTMKAC 1 cut(s) 1710
FokI GGATG 7 cut(s) 259, 513, 744, 882, 934, 955, 1381
FspAI RTGCGCAY 1 cut(s) 1741
FspBI CTAG 7 cut(s) 390, 684, 702, 989, 1026, 1602, 1623
FspI TGCGCA 1 cut(s) 1741
GlaI GCGC 1 cut(s) 1741
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 5 cut(s) 616, 920, 926, 987, 1041
HgaI GACGC 1 cut(s) 1702
HhaI GCGC 1 cut(s) 1742
Hin1I GRCGYC 1 cut(s) 1694
Hin6I GCGC 1 cut(s) 1740
HinP1I GCGC 1 cut(s) 1740
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HinfI GANTC 3 cut(s) 101, 1685, 1731
HphI GGTGA 6 cut(s) 107, 801, 1048, 1147, 1694, 1764
Hpy166II GTNNAC 3 cut(s) 994, 1497, 1711
Hpy188III TCNNGA 7 cut(s) 223, 257, 398, 425, 533, 1083, 1421
Hpy8I GTNNAC 3 cut(s) 994, 1497, 1711
Hpy99I CGWCG 1 cut(s) 817
HpyAV CCTTC 4 cut(s) 101, 220, 538, 1215
HpyCH4III ACNGT 8 cut(s) 331, 797, 805, 813, 1098, 1104, 1121, 1348
HpyCH4IV ACGT 1 cut(s) 1574
HpyCH4V TGCA 8 cut(s) 4, 250, 488, 504, 801, 838, 1325, 1517
HpyF10VI GCNNNNNNNGC 4 cut(s) 485, 1472, 1523, 1562
HpyF3I CTNAG 7 cut(s) 362, 410, 606, 1196, 1458, 1482, 1542
HpySE526I ACGT 1 cut(s) 1574
Hsp92I GRCGYC 1 cut(s) 1694
HspAI GCGC 1 cut(s) 1740
Ksp22I TGATCA 4 cut(s) 316, 400, 529, 867
KspI CCGCGG 1 cut(s) 945
Kzo9I GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
LmnI GCTCC 5 cut(s) 563, 718, 1177, 1474, 1480
Lsp1109I GCAGC 7 cut(s) 508, 511, 1306, 1309, 1423, 1478, 1574
LweI GCATC 7 cut(s) 237, 491, 695, 700, 956, 1495, 1751
MaeI CTAG 7 cut(s) 390, 684, 702, 989, 1026, 1602, 1623
MaeII ACGT 1 cut(s) 1574
MaeIII GTNAC 6 cut(s) 601, 884, 1028, 1098, 1625, 1770
MalI GATC 7 cut(s) 318, 402, 420, 531, 585, 869, 1164
MboI GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
MboII GAAGA 9 cut(s) 197, 287, 605, 646, 688, 863, 1251, 1257, 1383
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 1 cut(s) 821
MlsI TGGCCA 1 cut(s) 1041
MluNI TGGCCA 1 cut(s) 1041
MlyI GAGTC 1 cut(s) 1694
MmeI TCCRAC 1 cut(s) 740
Mox20I TGGCCA 1 cut(s) 1041
Mph1103I ATGCAT 1 cut(s) 252
MroXI GAANNNNTTC 1 cut(s) 331
MscI TGGCCA 1 cut(s) 1041
MseI TTAA 6 cut(s) 549, 567, 1308, 1398, 1635, 1701
MslI CAYNNNNRTG 3 cut(s) 814, 920, 1041
Msp20I TGGCCA 1 cut(s) 1041
MspA1I CMGCKG 5 cut(s) 314, 521, 944, 1319, 1565
MspR9I CCNGG 2 cut(s) 236, 621
MvaI CCWGG 2 cut(s) 236, 621
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 4 cut(s) 485, 1472, 1523, 1562
NcoI CCATGG 3 cut(s) 921, 1036, 1042
NdeII GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
NmeAIII GCCGAG 1 cut(s) 1242
NmuCI GTSAC 3 cut(s) 1098, 1625, 1770
NsbI TGCGCA 1 cut(s) 1741
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 1 cut(s) 859
OliI CACNNNNGTG 1 cut(s) 814
PaeR7I CTCGAG 3 cut(s) 379, 783, 1228
PagI TCATGA 3 cut(s) 397, 424, 532
PceI AGGCCT 1 cut(s) 987
PciI ACATGT 1 cut(s) 855
PdmI GAANNNNTTC 1 cut(s) 331
PfeI GAWTC 2 cut(s) 101, 1731
PleI GAGTC 1 cut(s) 1693
PpsI GAGTC 1 cut(s) 1693
PscI ACATGT 1 cut(s) 855
PshBI ATTAAT 2 cut(s) 567, 1701
Psp6I CCWGG 2 cut(s) 234, 619
PspEI GGTNACC 1 cut(s) 884
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 2 cut(s) 234, 619
PspPI GGNCC 1 cut(s) 1046
PspXI VCTCGAGB 3 cut(s) 379, 783, 1228
PsrI GAACNNNNNNTAC 2 cut(s) 1642, 1674
PstI CTGCAG 1 cut(s) 1327
PstNI CAGNNNCTG 2 cut(s) 524, 1472
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 2 cut(s) 314, 521
RsaI GTAC 7 cut(s) 310, 388, 394, 1234, 1350, 1600, 1651
RsaNI GTAC 7 cut(s) 309, 387, 393, 1233, 1349, 1599, 1650
RseI CAYNNNNRTG 3 cut(s) 814, 920, 1041
SacII CCGCGG 1 cut(s) 945
SaqAI TTAA 6 cut(s) 549, 567, 1308, 1398, 1635, 1701
Sau3AI GATC 7 cut(s) 316, 400, 418, 529, 583, 867, 1162
Sau96I GGNCC 1 cut(s) 1046
ScaI AGTACT 3 cut(s) 388, 394, 1651
SchI GAGTC 1 cut(s) 1694
ScrFI CCNGG 2 cut(s) 236, 621
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 7 cut(s) 237, 491, 695, 700, 956, 1495, 1751
SfcI CTRYAG 1 cut(s) 1323
Sfr274I CTCGAG 3 cut(s) 379, 783, 1228
Sfr303I CCGCGG 1 cut(s) 945
SgrBI CCGCGG 1 cut(s) 945
SinI GGWCC 1 cut(s) 1046
SlaI CTCGAG 3 cut(s) 379, 783, 1228
SmiMI CAYNNNNRTG 3 cut(s) 814, 920, 1041
SmlI CTYRAG 3 cut(s) 379, 783, 1228
SmoI CTYRAG 3 cut(s) 379, 783, 1228
SpeI ACTAGT 3 cut(s) 389, 1025, 1622
SseBI AGGCCT 1 cut(s) 987
SspMI CTAG 7 cut(s) 390, 684, 702, 989, 1026, 1602, 1623
StuI AGGCCT 1 cut(s) 987
StyD4I CCNGG 2 cut(s) 234, 619
StyI CCWWGG 4 cut(s) 367, 921, 1036, 1042
TaaI ACNGT 8 cut(s) 331, 797, 805, 813, 1098, 1104, 1121, 1348
TaiI ACGT 1 cut(s) 1577
TaqI TCGA 6 cut(s) 380, 784, 860, 1082, 1229, 1272
TatI WGTACW 5 cut(s) 308, 386, 392, 1232, 1649
TauI GCSGC 5 cut(s) 581, 619, 944, 947, 1568
TfiI GAWTC 2 cut(s) 101, 1731
Tru1I TTAA 6 cut(s) 549, 567, 1308, 1398, 1635, 1701
Tru9I TTAA 6 cut(s) 549, 567, 1308, 1398, 1635, 1701
TscAI CASTG 2 cut(s) 1174, 1519
TseFI GTSAC 3 cut(s) 1098, 1625, 1770
TseI GCWGC 7 cut(s) 521, 524, 1319, 1322, 1411, 1466, 1562
Tsp45I GTSAC 3 cut(s) 1098, 1625, 1770
TspGWI ACGGA 1 cut(s) 1419
TspRI CASTG 2 cut(s) 1174, 1519
VpaK11BI GGWCC 1 cut(s) 1046
VspI ATTAAT 2 cut(s) 567, 1701
XapI RAATTY 3 cut(s) 58, 1075, 1453
XceI RCATGY 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 1382
XhoI CTCGAG 3 cut(s) 379, 783, 1228
XmiI GTMKAC 1 cut(s) 1710
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 7 cut(s) 390, 684, 702, 989, 1026, 1602, 1623
ZrmI AGTACT 3 cut(s) 388, 394, 1651
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.