RchiOBHm_Chr4g0440391
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008989
Physical Location & Seq
Reverse (-)
62515413 .. 62517361
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40836

Sequence Viewer

Length: 1668 bp
ATGCCCATGCAGGAAGCAGATGATGAACTGGAATTAGCTAAAGCTGAAATGGGGGAGGTTAGAGAAGAAAATAAAAGGCTAAAGGTGTTATTATCGCAAATCGTGAAGGATTACCAGGAACTTCAGATGCATTTTCTTGATGTTATTCAAAAAGAAGAAACAAATAAGAAATCTATGGATACCAGTAGTACAGCTGATCATCAAGGAACTGTTTCGGCTGAAGCTGATGAACTTGTGTCCCTTAGCCTTGGGAGAACCTCGAGTAGTACTAGTACTCATGATCATCAGCTCAGAAAGGATCATCATGAGATGATGAAAAAGACCAGCAGAGATGACTATTACGGGGTAATGAATGAAGCTGGACTTGCATTGGAATTGGGATGCAGATCATTTGAGCCAGCTGCTGCTGATCATGAAACTATGAAGGTTAATTCAAGCTCCGAAATTAATAGTTCAGCCGCAGATCCAAAGGAAGACGAAGTAACTAAGATATGGCCGCCTGGTAAAATGTTGAAGACAAGAGATGATGATGTTTCACAACAGCAGACCCATTTGAAGAAAGCTAGGGTTTCTGTCCGAGCTAGATGCGATGCTCCAACATTGAATGATGGATGTCAATGGAGAAAATATGGACAGAAAATAGCAAAAGGAAATCCATGCCCTCGAGCATACTACCGTTGCACAGTTTCACCGTCGTGCCCTGTGAGAAAACAAGTGCAAAGATGTGCGGAAGACATGTCGATATTGATCACAACCTATGAGGGTAACCACAACCATCCACTTCCCATGTCAGCCACGGCCATGGCCTCCACCACCTCCGCCGCGGCATCCATGCTTCAGTCTCACTCATCCACCTCGCAACAAGGCCTTGTCAACTCAACCACCGCCCCCATCTCCACCTCCACTAGTAACCTCCATGGCCATGGACCAAATTACTTCACCAATAGTCTCTCCCAGAATTCATCGAGACTACTACCACAGTCACAGTTCTATTTCCCCAACAGTTCAATCTCAACCAACAATTCCCACCCAACCATCACCCTTGATCTCACTGCTCCATCTCCCTCCCATTTCGGACTAAGATTTCCTTCAGGATTACCGAGATATTCCTCGAGTACAAGCCTCAACTTTTCGTCATCGTCTTCCTCTTCTTTCGACCACAACACATTACTACAAACACCAAATTGGATTAACAATCCCGCTGCTGCAGGCTATTTCAACTATGGGACGGTACTATCTCATCAGAATAGGATGAACCAAGCTGTTGGTGGGTCTTCTCTTAACAACGGAAAGCAGCCATTTCAAGAACCTAATTTCTACCAATCTTACATAAAAAATTCTCAGAAAGCAGCTCCTGCTCCACCTCAGCATCATCTGTTTACAGAGACATTAACCACTGCAACAAAAGCAATCACATCAAACCCTAAGTTTCAATCAGCTTTAGCAGCGGCACTCACGTCATTTGTTGGTGCTAATGGGGGTACTAGCGGGGCGGTCAGAGAAACTAGTCACATCGTTAATGGCAGTAGTAGTACTGAAAGTTCTGGGCTGAAATTGAAGTGGGGTGAGTCCTTGACGCCGATTAATCCAATCTACCCATCAACCCAGAAAGGAATCGGGTGCGCATCATCAGGCTACTTGAACAAATCATCGTCGCCATTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

555

Amino Acids

60.36

Weight (kDa)

7.68

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 202 - 260 1.5e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1624
AccII CGCG 1 cut(s) 824
AclWI GGATC 2 cut(s) 306, 458
AcoI YGGCCR 3 cut(s) 494, 798, 919
AcsI RAATTY 2 cut(s) 958, 1336
AcuI CTGAAG 4 cut(s) 107, 240, 821, 1074
AcyI GRCGYC 1 cut(s) 1577
AfaI GTAC 7 cut(s) 190, 268, 274, 1117, 1233, 1483, 1534
AflIII ACRYGT 1 cut(s) 735
AhlI ACTAGT 3 cut(s) 269, 905, 1505
AjiI CACGTC 1 cut(s) 1458
AjnI CCWGG 2 cut(s) 114, 499
AleI CACNNNNGTG 1 cut(s) 694
Alw26I GTCTC 4 cut(s) 846, 953, 961, 1379
AlwI GGATC 2 cut(s) 306, 458
AlwNI CAGNNNCTG 2 cut(s) 404, 1355
Ama87I CYCGRG 3 cut(s) 259, 663, 1111
AoxI GGCC 5 cut(s) 494, 798, 804, 865, 919
ApeKI GCWGC 7 cut(s) 401, 404, 1202, 1205, 1294, 1349, 1445
ApoI RAATTY 2 cut(s) 958, 1336
AseI ATTAAT 2 cut(s) 447, 1584
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 1 cut(s) 1625
AspS9I GGNCC 1 cut(s) 926
AsuHPI GGTGA 4 cut(s) 681, 931, 1030, 1577
AvaI CYCGRG 3 cut(s) 259, 663, 1111
AvaII GGWCC 1 cut(s) 926
BaeGI GKGCMC 1 cut(s) 701
BalI TGGCCA 1 cut(s) 921
BbsI GAAGAC 5 cut(s) 480, 521, 738, 1134, 1266
BbvCI CCTCAGC 1 cut(s) 1365
BbvI GCAGC 7 cut(s) 388, 391, 1189, 1192, 1306, 1361, 1457
BccI CCATC 6 cut(s) 602, 783, 899, 1043, 1066, 1606
BceAI ACGGC 1 cut(s) 813
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 2 cut(s) 116, 501
BciVI GTATCC 1 cut(s) 172
BclI TGATCA 4 cut(s) 196, 280, 409, 747
BcoDI GTCTC 4 cut(s) 846, 953, 961, 1379
BcuI ACTAGT 3 cut(s) 269, 905, 1505
BfaI CTAG 6 cut(s) 270, 564, 582, 906, 1485, 1506
BfmI CTRYAG 1 cut(s) 1206
BfuI GTATCC 1 cut(s) 172
BmcAI AGTACT 3 cut(s) 268, 274, 1534
Bme1390I CCNGG 2 cut(s) 116, 501
Bme18I GGWCC 1 cut(s) 926
BmeT110I CYCGRG 3 cut(s) 259, 663, 1111
BmgBI CACGTC 1 cut(s) 1458
BmgT120I GGNCC 1 cut(s) 926
BmrFI CCNGG 2 cut(s) 116, 501
BmsI GCATC 7 cut(s) 117, 371, 575, 580, 836, 1378, 1634
BpiI GAAGAC 5 cut(s) 480, 521, 738, 1134, 1266
Bpu10I CCTNAGC 2 cut(s) 242, 1365
BsaBI GATNNNNATC 2 cut(s) 385, 746
BsaHI GRCGYC 1 cut(s) 1577
BsaJI CCNNGG 6 cut(s) 247, 795, 801, 822, 916, 922
Bse1I ACTGG 2 cut(s) 33, 183
Bse8I GATNNNNATC 2 cut(s) 385, 746
BseBI CCWGG 2 cut(s) 116, 501
BseDI CCNNGG 6 cut(s) 247, 795, 801, 822, 916, 922
BseGI GGATG 6 cut(s) 386, 617, 775, 827, 848, 1257
BseJI GATNNNNATC 2 cut(s) 385, 746
BseMII CTCAG 3 cut(s) 304, 1355, 1379
BseNI ACTGG 2 cut(s) 33, 183
BseSI GKGCMC 1 cut(s) 701
BseXI GCAGC 7 cut(s) 388, 391, 1189, 1192, 1306, 1361, 1457
Bsh1236I CGCG 1 cut(s) 824
BshFI GGCC 5 cut(s) 496, 800, 806, 867, 921
BsiHKCI CYCGRG 3 cut(s) 259, 663, 1111
BslFI GGGAC 2 cut(s) 223, 1240
BsmAI GTCTC 4 cut(s) 846, 953, 961, 1379
BsmFI GGGAC 2 cut(s) 223, 1240
BsnI GGCC 5 cut(s) 496, 800, 806, 867, 921
BsoBI CYCGRG 3 cut(s) 259, 663, 1111
Bsp1286I GDGCHC 1 cut(s) 701
Bsp143I GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
Bsp19I CCATGG 3 cut(s) 801, 916, 922
BspANI GGCC 5 cut(s) 496, 800, 806, 867, 921
BspCNI CTCAG 3 cut(s) 303, 1354, 1378
BspFNI CGCG 1 cut(s) 824
BspHI TCATGA 3 cut(s) 277, 304, 412
BspMAI CTGCAG 1 cut(s) 1210
BspPI GGATC 2 cut(s) 306, 458
BsrI ACTGG 2 cut(s) 33, 183
BssECI CCNNGG 6 cut(s) 247, 795, 801, 822, 916, 922
BssMI GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
BssNI GRCGYC 1 cut(s) 1577
BssT1I CCWWGG 4 cut(s) 247, 801, 916, 922
Bst2UI CCWGG 2 cut(s) 116, 501
Bst4CI ACNGT 8 cut(s) 211, 677, 685, 693, 981, 987, 1004, 1231
Bst6I CTCTTC 1 cut(s) 1153
BstACI GRCGYC 1 cut(s) 1577
BstAPI GCANNNNNTGC 1 cut(s) 1355
BstC8I GCNNGC 2 cut(s) 399, 1210
BstDEI CTNAG 7 cut(s) 242, 290, 486, 1079, 1341, 1365, 1425
BstDSI CCRYGG 5 cut(s) 795, 801, 822, 916, 922
BstEII GGTNACC 1 cut(s) 764
BstF5I GGATG 6 cut(s) 386, 617, 775, 827, 848, 1257
BstFNI CGCG 1 cut(s) 824
BstHHI GCGC 1 cut(s) 1625
BstKTI GATC 8 cut(s) 199, 283, 301, 389, 412, 466, 750, 1048
BstMAI GTCTC 4 cut(s) 846, 953, 961, 1379
BstMBI GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 365, 1355, 1406, 1445
BstNI CCWGG 2 cut(s) 116, 501
BstNSI RCATGY 1 cut(s) 739
BstPI GGTNACC 1 cut(s) 764
BstSCI CCNGG 2 cut(s) 114, 499
BstSFI CTRYAG 1 cut(s) 1206
BstSLI GKGCMC 1 cut(s) 701
BstUI CGCG 1 cut(s) 824
BstV1I GCAGC 7 cut(s) 388, 391, 1189, 1192, 1306, 1361, 1457
BstV2I GAAGAC 5 cut(s) 480, 521, 738, 1134, 1266
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 3 cut(s) 802, 923, 1265
BstYI RGATCY 1 cut(s) 463
BsuI GTATCC 1 cut(s) 172
BsuRI GGCC 5 cut(s) 496, 800, 806, 867, 921
BtgI CCRYGG 5 cut(s) 795, 801, 822, 916, 922
BtgZI GCGATG 1 cut(s) 603
BtrI CACGTC 1 cut(s) 1458
BtsCI GGATG 6 cut(s) 386, 617, 775, 827, 848, 1257
BtsI GCAGTG 2 cut(s) 1050, 1395
BtsIMutI CAGTG 2 cut(s) 1050, 1395
Cac8I GCNNGC 2 cut(s) 399, 1210
CaiI CAGNNNCTG 2 cut(s) 404, 1355
CciI TCATGA 3 cut(s) 277, 304, 412
CfoI GCGC 1 cut(s) 1625
Cfr13I GGNCC 1 cut(s) 926
Cfr42I CCGCGG 1 cut(s) 825
CseI GACGC 1 cut(s) 1585
Csp6I GTAC 7 cut(s) 189, 267, 273, 1116, 1232, 1482, 1533
CviQI GTAC 7 cut(s) 189, 267, 273, 1116, 1232, 1482, 1533
DdeI CTNAG 7 cut(s) 242, 290, 486, 1079, 1341, 1365, 1425
DpnI GATC 8 cut(s) 198, 282, 300, 388, 411, 465, 749, 1047
DpnII GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
EaeI YGGCCR 3 cut(s) 494, 798, 919
Eam1104I CTCTTC 1 cut(s) 1153
EarI CTCTTC 1 cut(s) 1153
EciI GGCGGA 1 cut(s) 808
Eco130I CCWWGG 4 cut(s) 247, 801, 916, 922
Eco147I AGGCCT 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 926
Eco57I CTGAAG 4 cut(s) 107, 240, 821, 1074
Eco88I CYCGRG 3 cut(s) 259, 663, 1111
Eco91I GGTNACC 1 cut(s) 764
EcoO65I GGTNACC 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 958
EcoRII CCWGG 2 cut(s) 114, 499
EcoT14I CCWWGG 4 cut(s) 247, 801, 916, 922
EcoT22I ATGCAT 1 cut(s) 132
ErhI CCWWGG 4 cut(s) 247, 801, 916, 922
FalI AAGNNNNNCTT 4 cut(s) 348, 380, 1072, 1104
FaqI GGGAC 2 cut(s) 223, 1240
FauI CCCGC 2 cut(s) 1207, 1481
FbaI TGATCA 4 cut(s) 196, 280, 409, 747
FokI GGATG 6 cut(s) 393, 624, 762, 814, 835, 1264
FspAI RTGCGCAY 1 cut(s) 1624
FspBI CTAG 6 cut(s) 270, 564, 582, 906, 1485, 1506
FspI TGCGCA 1 cut(s) 1624
GlaI GCGC 1 cut(s) 1624
HaeIII GGCC 5 cut(s) 496, 800, 806, 867, 921
HgaI GACGC 1 cut(s) 1585
HhaI GCGC 1 cut(s) 1625
Hin1I GRCGYC 1 cut(s) 1577
Hin6I GCGC 1 cut(s) 1623
HinP1I GCGC 1 cut(s) 1623
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HinfI GANTC 2 cut(s) 1568, 1614
HphI GGTGA 4 cut(s) 681, 931, 1030, 1577
Hpy166II GTNNAC 2 cut(s) 874, 1380
Hpy188I TCNGA 8 cut(s) 126, 293, 442, 578, 1076, 1245, 1344, 1499
Hpy188III TCNNGA 8 cut(s) 103, 137, 278, 305, 413, 966, 1092, 1304
Hpy8I GTNNAC 2 cut(s) 874, 1380
Hpy99I CGWCG 2 cut(s) 697, 1657
HpyAV CCTTC 3 cut(s) 100, 418, 1098
HpyCH4III ACNGT 8 cut(s) 211, 677, 685, 693, 981, 987, 1004, 1231
HpyCH4IV ACGT 1 cut(s) 1457
HpyCH4V TGCA 8 cut(s) 10, 130, 368, 384, 681, 718, 1208, 1400
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 1355, 1406, 1445
HpyF3I CTNAG 7 cut(s) 242, 290, 486, 1079, 1341, 1365, 1425
HpySE526I ACGT 1 cut(s) 1457
Hsp92I GRCGYC 1 cut(s) 1577
HspAI GCGC 1 cut(s) 1623
Ksp22I TGATCA 4 cut(s) 196, 280, 409, 747
KspI CCGCGG 1 cut(s) 825
Kzo9I GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
LmnI GCTCC 5 cut(s) 443, 598, 1060, 1357, 1363
Lsp1109I GCAGC 7 cut(s) 388, 391, 1189, 1192, 1306, 1361, 1457
LweI GCATC 7 cut(s) 117, 371, 575, 580, 836, 1378, 1634
MaeI CTAG 6 cut(s) 270, 564, 582, 906, 1485, 1506
MaeII ACGT 1 cut(s) 1457
MaeIII GTNAC 5 cut(s) 481, 764, 908, 981, 1508
MalI GATC 8 cut(s) 198, 282, 300, 388, 411, 465, 749, 1047
MboI GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
MboII GAAGA 9 cut(s) 77, 167, 485, 526, 568, 743, 1134, 1140, 1266
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 1 cut(s) 701
MlsI TGGCCA 1 cut(s) 921
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 1 cut(s) 1577
MmeI TCCRAC 1 cut(s) 620
Mox20I TGGCCA 1 cut(s) 921
Mph1103I ATGCAT 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 211
MscI TGGCCA 1 cut(s) 921
MseI TTAA 7 cut(s) 429, 447, 1191, 1281, 1391, 1518, 1584
MslI CAYNNNNRTG 3 cut(s) 694, 800, 921
Msp20I TGGCCA 1 cut(s) 921
MspA1I CMGCKG 5 cut(s) 194, 401, 824, 1202, 1448
MspR9I CCNGG 2 cut(s) 116, 501
MvaI CCWGG 2 cut(s) 116, 501
MvnI CGCG 1 cut(s) 824
MwoI GCNNNNNNNGC 4 cut(s) 365, 1355, 1406, 1445
NcoI CCATGG 3 cut(s) 801, 916, 922
NdeII GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
NmuCI GTSAC 2 cut(s) 981, 1508
NsbI TGCGCA 1 cut(s) 1624
NsiI ATGCAT 1 cut(s) 132
NspI RCATGY 1 cut(s) 739
OliI CACNNNNGTG 1 cut(s) 694
PaeR7I CTCGAG 3 cut(s) 259, 663, 1111
PagI TCATGA 3 cut(s) 277, 304, 412
PceI AGGCCT 1 cut(s) 867
PciI ACATGT 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 1614
PleI GAGTC 1 cut(s) 1576
PpsI GAGTC 1 cut(s) 1576
PscI ACATGT 1 cut(s) 735
PshBI ATTAAT 2 cut(s) 447, 1584
Psp6I CCWGG 2 cut(s) 114, 499
PspEI GGTNACC 1 cut(s) 764
PspGI CCWGG 2 cut(s) 114, 499
PspPI GGNCC 1 cut(s) 926
PspXI VCTCGAGB 3 cut(s) 259, 663, 1111
PsrI GAACNNNNNNTAC 2 cut(s) 1525, 1557
PstI CTGCAG 1 cut(s) 1210
PstNI CAGNNNCTG 2 cut(s) 404, 1355
PsuI RGATCY 1 cut(s) 463
PvuII CAGCTG 2 cut(s) 194, 401
RsaI GTAC 7 cut(s) 190, 268, 274, 1117, 1233, 1483, 1534
RsaNI GTAC 7 cut(s) 189, 267, 273, 1116, 1232, 1482, 1533
RseI CAYNNNNRTG 3 cut(s) 694, 800, 921
SacII CCGCGG 1 cut(s) 825
SaqAI TTAA 7 cut(s) 429, 447, 1191, 1281, 1391, 1518, 1584
Sau3AI GATC 8 cut(s) 196, 280, 298, 386, 409, 463, 747, 1045
Sau96I GGNCC 1 cut(s) 926
ScaI AGTACT 3 cut(s) 268, 274, 1534
SchI GAGTC 1 cut(s) 1577
ScrFI CCNGG 2 cut(s) 116, 501
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 7 cut(s) 117, 371, 575, 580, 836, 1378, 1634
SfcI CTRYAG 1 cut(s) 1206
Sfr274I CTCGAG 3 cut(s) 259, 663, 1111
Sfr303I CCGCGG 1 cut(s) 825
SgrBI CCGCGG 1 cut(s) 825
SinI GGWCC 1 cut(s) 926
SlaI CTCGAG 3 cut(s) 259, 663, 1111
SmiMI CAYNNNNRTG 3 cut(s) 694, 800, 921
SmlI CTYRAG 3 cut(s) 259, 663, 1111
SmoI CTYRAG 3 cut(s) 259, 663, 1111
SpeI ACTAGT 3 cut(s) 269, 905, 1505
SseBI AGGCCT 1 cut(s) 867
SspMI CTAG 6 cut(s) 270, 564, 582, 906, 1485, 1506
StuI AGGCCT 1 cut(s) 867
StyD4I CCNGG 2 cut(s) 114, 499
StyI CCWWGG 4 cut(s) 247, 801, 916, 922
TaaI ACNGT 8 cut(s) 211, 677, 685, 693, 981, 987, 1004, 1231
TaiI ACGT 1 cut(s) 1460
TaqI TCGA 6 cut(s) 260, 664, 740, 965, 1112, 1155
TatI WGTACW 5 cut(s) 188, 266, 272, 1115, 1532
TauI GCSGC 5 cut(s) 461, 499, 824, 827, 1451
TfiI GAWTC 1 cut(s) 1614
Tru1I TTAA 7 cut(s) 429, 447, 1191, 1281, 1391, 1518, 1584
Tru9I TTAA 7 cut(s) 429, 447, 1191, 1281, 1391, 1518, 1584
TscAI CASTG 2 cut(s) 1057, 1402
TseFI GTSAC 2 cut(s) 981, 1508
TseI GCWGC 7 cut(s) 401, 404, 1202, 1205, 1294, 1349, 1445
Tsp45I GTSAC 2 cut(s) 981, 1508
TspDTI ATGAA 9 cut(s) 39, 243, 329, 365, 369, 429, 437, 951, 1268
TspGWI ACGGA 1 cut(s) 1302
TspRI CASTG 2 cut(s) 1057, 1402
VpaK11BI GGWCC 1 cut(s) 926
VspI ATTAAT 2 cut(s) 447, 1584
XapI RAATTY 2 cut(s) 958, 1336
XceI RCATGY 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 1265
XhoI CTCGAG 3 cut(s) 259, 663, 1111
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 6 cut(s) 270, 564, 582, 906, 1485, 1506
ZrmI AGTACT 3 cut(s) 268, 274, 1534
Zsp2I ATGCAT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.