FvH4_4g31790
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008989
Physical Location & Seq
Reverse (-)
30768133 .. 30771798
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31790.t2

Sequence Viewer

Length: 1848 bp
ATGCAAATAGCAAAGAGGCGTTCAAAATCAATGGAGATTGATAAGTCTTTGCTGGAAACTTCAGTTATGGAAAGCAACAGAATGAAATCTGATGGTGATGATGATTCAGAAGGCACTTCCAAAGATATAAACAAGGAAGGACATGTTGAGGAAGTAGCTCATGAAGGTGAATACAAGTTGAAGCTGCAGCCTTCAACTACGCAAAAGCATAGAGCTGATGAGAGCAAGCACAAACAGGAAGCCGATCATGATGAACTGGAATCAGCCAAAGCTGAAATGGGGAAGGTTAGAGAAGAAAATGAAAGGTTAAAGTTGTTTTTAGCTCGAACCCTGAAGGATTACCAGGATCTTCAGATGCATTTTCTTGATGTTATTCAGAAAGAAGAAACAAAAAAGAAGACTGTGGATACTAGTAGTACTGCTGAACATCAAGGAACTATTTCGGCTGAAGCTGATGAACTTGTGTCCCTTAGCCTTGGGAGAACCTCGAGTACTGCTAGTACTCATGATCATCAGCTCAGAAAGGATAATCATGAGATGATGATGAAAAAGACCAGCAGAGACAACGACGACGAGGTAATGAATGAAGCTGGACTTGCCTTGGAACTGGGCCGCAGATCATTTGAGCCAGCTGCTTATGATCAGGATACTATGAAGGTTCATTCAAGCTCTGAAAATAATAGTTCAGCTCATGGGGATCCAAAGGAAGATGAAGTAACTGAGATATGGCCACCTAGTAAAATGTTGAAGACAAGAGAGGATGATGTTTCACAACAGCTGACCCATTTGAAGAAAGCTAGGGTTTCTGTCCGAGCTAGATGTGATGCTCCAACATTAAACGATGGATGCCAATGGAGAAAATATGGTCAGAAAATAGCGAAAGGAAACCCATGCCCTCGAGCATACTACCGTTGCACAGTCTCACCGTCGTGCCCTGTGAGAAAACAAGTGCAAAGATGTGCGGAAGACATGTCGATATTGATCACAACCTATGAGGGTAACCACAACCACCCACTTCCGATGTCAGCCACGGCTATGGCCTCCACCACCTCCGCCGCGGCATCCATGCTTCAGTCTCACTCCTCTACCTCGCAACAAGGTCTAGTCAACTCAACCACGGCCCCCGTCTCCACCTCCAGTAGTAACCCCTATGGCCATGGACCAAACTTCACCAATAGTCTCTCCCAAAATTCATCGAGACTACTACCACAGTCACAGTTCTATTTCCCCAACAGTACAATCTCAACCAACAATTCCCACCCAACCATCACCCTTGATCTCACTGCTCCACCCCCCTCCCAATTCGGACCAAGATTTCCTTCAGGGTTACCTAGGTATTCCTTGAGTACAAGCCTCAACTTTTCGTCATCGTCTTCCTCTTCTTTAGACCACAACACATTACTACAAGCACCAAACTGGATTAACAATCACGCTGCTGCAGGATACTTCAACTATGGGACAACACTTTCTCATCATCATAATAGGATGAACCAAGCCGTTGGCGGGTCTTCTCTTAACATTGGAAAGCACCCATTTCAAGAACCTAATTTCTACCATTCTTACATACAAAATTCTCAGAAAGCAGCTCCTACTCCACCTCAACATCATCTGTTTACAGAGACAATAGCCACTGCAACGAAAGCAATCACATCAAACCCTAAGTTTCAATCAGCATTAGCGGCGGCACTCATATCATTTGTTGGTACTAATGGGAGTACTGGCTGCGCGGTTAGAGAAAATAGTCACATCGTTAATGGCAGTAGCAGTAGTGAAAGTTCTGGTCTGAAATTGAAGTGCCCACTTGAACAACCCAGAACGGAATCGGGTGTGCATCATCAGGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

616

Amino Acids

67.73

Weight (kDa)

6.67

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 249 - 307 1.6e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1058, 1727
AciI CCGC 9 cut(s) 613, 962, 1053, 1056, 1058, 1503, 1679, 1682, 1727
AclWI GGATC 3 cut(s) 354, 692, 705
AcoI YGGCCR 2 cut(s) 728, 1153
AcsI RAATTY 2 cut(s) 1189, 1570
AcuI CTGAAG 6 cut(s) 45, 335, 353, 468, 1055, 1305
AfaI GTAC 7 cut(s) 418, 493, 502, 1237, 1348, 1705, 1717
AfiI CCNNNNNNNGG 1 cut(s) 1503
AflIII ACRYGT 2 cut(s) 142, 969
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 1 cut(s) 342
AjuI GAANNNNNNNTTGG 2 cut(s) 1303, 1335
AleI CACNNNNGTG 1 cut(s) 928
Alw26I GTCTC 7 cut(s) 555, 925, 1080, 1132, 1184, 1192, 1613
AlwI GGATC 3 cut(s) 354, 692, 705
Ama87I CYCGRG 2 cut(s) 487, 897
AoxI GGCC 5 cut(s) 610, 728, 1038, 1119, 1153
ApeKI GCWGC 7 cut(s) 184, 187, 632, 1433, 1436, 1583, 1722
ApoI RAATTY 2 cut(s) 1189, 1570
Asp700I GAANNNNTTC 1 cut(s) 439
AspA2I CCTAGG 1 cut(s) 1331
AspLEI GCGC 1 cut(s) 1727
AspS9I GGNCC 4 cut(s) 610, 1120, 1160, 1307
AsuHPI GGTGA 5 cut(s) 107, 179, 915, 1162, 1261
AvaI CYCGRG 2 cut(s) 487, 897
AvaII GGWCC 2 cut(s) 1160, 1307
AvrII CCTAGG 1 cut(s) 1331
BaeGI GKGCMC 2 cut(s) 935, 1799
BalI TGGCCA 2 cut(s) 730, 1155
BamHI GGATCC 1 cut(s) 697
BbsI GAAGAC 5 cut(s) 404, 755, 972, 1365, 1500
BbvI GCAGC 7 cut(s) 171, 199, 619, 1420, 1423, 1595, 1709
BccI CCATC 3 cut(s) 86, 836, 1274
BceAI ACGGC 3 cut(s) 1047, 1134, 1481
BciT130I CCWGG 1 cut(s) 344
BciVI GTATCC 3 cut(s) 400, 640, 1436
BclI TGATCA 3 cut(s) 508, 640, 981
BcoDI GTCTC 7 cut(s) 555, 925, 1080, 1132, 1184, 1192, 1613
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 7 cut(s) 411, 498, 735, 798, 816, 1103, 1332
BfmI CTRYAG 2 cut(s) 185, 1437
BfuI GTATCC 3 cut(s) 400, 640, 1436
BlnI CCTAGG 1 cut(s) 1331
BmcAI AGTACT 4 cut(s) 418, 493, 502, 1717
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 2 cut(s) 1160, 1307
BmeT110I CYCGRG 2 cut(s) 487, 897
BmgT120I GGNCC 4 cut(s) 610, 1120, 1160, 1307
BmiI GGNNCC 2 cut(s) 699, 1122
BmrFI CCNGG 1 cut(s) 344
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 5 cut(s) 345, 814, 836, 1070, 1840
BmuI ACTGGG 1 cut(s) 617
BpiI GAAGAC 5 cut(s) 404, 755, 972, 1365, 1500
BpmI CTGGAG 1 cut(s) 1120
Bpu10I CCTNAGC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 1363
BsaBI GATNNNNATC 1 cut(s) 980
BsaJI CCNNGG 7 cut(s) 475, 600, 1029, 1056, 1116, 1156, 1331
Bsc4I CCNNNNNNNGG 1 cut(s) 1503
Bse1I ACTGG 5 cut(s) 261, 612, 1137, 1421, 1723
Bse8I GATNNNNATC 1 cut(s) 980
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 7 cut(s) 475, 600, 1029, 1056, 1116, 1156, 1331
BseGI GGATG 4 cut(s) 766, 851, 1061, 1491
BseJI GATNNNNATC 1 cut(s) 980
BseLI CCNNNNNNNGG 1 cut(s) 1503
BseMII CTCAG 3 cut(s) 532, 711, 1589
BseNI ACTGG 5 cut(s) 261, 612, 1137, 1421, 1723
BseRI GAGGAG 1 cut(s) 1072
BseSI GKGCMC 2 cut(s) 935, 1799
BseXI GCAGC 7 cut(s) 171, 199, 619, 1420, 1423, 1595, 1709
Bsh1236I CGCG 2 cut(s) 1058, 1727
BshFI GGCC 5 cut(s) 612, 730, 1040, 1121, 1155
BsiHKCI CYCGRG 2 cut(s) 487, 897
BslFI GGGAC 2 cut(s) 451, 1471
BslI CCNNNNNNNGG 1 cut(s) 1503
BsmAI GTCTC 7 cut(s) 555, 925, 1080, 1132, 1184, 1192, 1613
BsmBI CGTCTC 1 cut(s) 1132
BsmFI GGGAC 2 cut(s) 451, 1471
BsnI GGCC 5 cut(s) 612, 730, 1040, 1121, 1155
BsoBI CYCGRG 2 cut(s) 487, 897
Bsp1286I GDGCHC 2 cut(s) 935, 1799
Bsp143I GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
Bsp19I CCATGG 1 cut(s) 1156
BspACI CCGC 9 cut(s) 613, 962, 1053, 1056, 1058, 1503, 1679, 1682, 1727
BspANI GGCC 5 cut(s) 612, 730, 1040, 1121, 1155
BspCNI CTCAG 3 cut(s) 531, 712, 1588
BspFNI CGCG 2 cut(s) 1058, 1727
BspHI TCATGA 4 cut(s) 160, 247, 505, 532
BspLI GGNNCC 2 cut(s) 699, 1122
BspMAI CTGCAG 2 cut(s) 189, 1441
BspPI GGATC 3 cut(s) 354, 692, 705
BsrI ACTGG 5 cut(s) 261, 612, 1137, 1421, 1723
BssECI CCNNGG 7 cut(s) 475, 600, 1029, 1056, 1116, 1156, 1331
BssMI GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
BssT1I CCWWGG 4 cut(s) 475, 600, 1156, 1331
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 7 cut(s) 403, 911, 919, 927, 1212, 1218, 1235
Bst6I CTCTTC 1 cut(s) 1384
BstC8I GCNNGC 2 cut(s) 227, 630
BstDEI CTNAG 5 cut(s) 470, 518, 720, 1575, 1659
BstDSI CCRYGG 4 cut(s) 1029, 1056, 1116, 1156
BstEII GGTNACC 2 cut(s) 998, 1326
BstF5I GGATG 4 cut(s) 766, 851, 1061, 1491
BstFNI CGCG 2 cut(s) 1058, 1727
BstHHI GCGC 1 cut(s) 1727
BstKTI GATC 8 cut(s) 247, 349, 511, 620, 643, 700, 984, 1279
BstMAI GTCTC 7 cut(s) 555, 925, 1080, 1132, 1184, 1192, 1613
BstMBI GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
BstMWI GCNNNNNNNGC 3 cut(s) 596, 1640, 1679
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 2 cut(s) 146, 973
BstPI GGTNACC 2 cut(s) 998, 1326
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 2 cut(s) 185, 1437
BstSLI GKGCMC 2 cut(s) 935, 1799
BstUI CGCG 2 cut(s) 1058, 1727
BstV1I GCAGC 7 cut(s) 171, 199, 619, 1420, 1423, 1595, 1709
BstV2I GAAGAC 5 cut(s) 404, 755, 972, 1365, 1500
BstX2I RGATCY 2 cut(s) 346, 697
BstXI CCANNNNNNTGG 2 cut(s) 1036, 1499
BstYI RGATCY 2 cut(s) 346, 697
BsuI GTATCC 3 cut(s) 400, 640, 1436
BsuRI GGCC 5 cut(s) 612, 730, 1040, 1121, 1155
BtgI CCRYGG 4 cut(s) 1029, 1056, 1116, 1156
BtsCI GGATG 4 cut(s) 766, 851, 1061, 1491
BtsI GCAGTG 2 cut(s) 1281, 1629
BtsIMutI CAGTG 2 cut(s) 1281, 1629
Cac8I GCNNGC 2 cut(s) 227, 630
CciI TCATGA 4 cut(s) 160, 247, 505, 532
CfoI GCGC 1 cut(s) 1727
Cfr13I GGNCC 4 cut(s) 610, 1120, 1160, 1307
Cfr42I CCGCGG 1 cut(s) 1059
Csp6I GTAC 7 cut(s) 417, 492, 501, 1236, 1347, 1704, 1716
CviQI GTAC 7 cut(s) 417, 492, 501, 1236, 1347, 1704, 1716
DdeI CTNAG 5 cut(s) 470, 518, 720, 1575, 1659
DpnI GATC 8 cut(s) 246, 348, 510, 619, 642, 699, 983, 1278
DpnII GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
EaeI YGGCCR 2 cut(s) 728, 1153
Eam1104I CTCTTC 1 cut(s) 1384
EarI CTCTTC 1 cut(s) 1384
EciI GGCGGA 1 cut(s) 1042
Eco130I CCWWGG 4 cut(s) 475, 600, 1156, 1331
Eco47I GGWCC 2 cut(s) 1160, 1307
Eco57I CTGAAG 6 cut(s) 45, 335, 353, 468, 1055, 1305
Eco88I CYCGRG 2 cut(s) 487, 897
Eco91I GGTNACC 2 cut(s) 998, 1326
EcoO65I GGTNACC 2 cut(s) 998, 1326
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 4 cut(s) 475, 600, 1156, 1331
EcoT22I ATGCAT 1 cut(s) 360
ErhI CCWWGG 4 cut(s) 475, 600, 1156, 1331
Esp3I CGTCTC 1 cut(s) 1132
FalI AAGNNNNNCTT 4 cut(s) 579, 611, 1303, 1335
FaqI GGGAC 2 cut(s) 451, 1471
FauI CCCGC 1 cut(s) 1496
FbaI TGATCA 3 cut(s) 508, 640, 981
FokI GGATG 4 cut(s) 773, 858, 1048, 1498
FspBI CTAG 7 cut(s) 411, 498, 735, 798, 816, 1103, 1332
GlaI GCGC 1 cut(s) 1726
GsuI CTGGAG 1 cut(s) 1120
HaeIII GGCC 5 cut(s) 612, 730, 1040, 1121, 1155
HhaI GCGC 1 cut(s) 1727
Hin6I GCGC 1 cut(s) 1725
HinP1I GCGC 1 cut(s) 1725
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HinfI GANTC 3 cut(s) 104, 260, 1820
HphI GGTGA 5 cut(s) 107, 179, 915, 1162, 1261
Hpy166II GTNNAC 2 cut(s) 1108, 1614
Hpy188III TCNNGA 8 cut(s) 161, 248, 365, 506, 533, 644, 1197, 1538
Hpy8I GTNNAC 2 cut(s) 1108, 1614
Hpy99I CGWCG 3 cut(s) 572, 575, 931
HpyAV CCTTC 8 cut(s) 104, 131, 158, 201, 277, 328, 649, 1329
HpyCH4III ACNGT 7 cut(s) 403, 911, 919, 927, 1212, 1218, 1235
HpyCH4V TGCA 8 cut(s) 4, 187, 358, 915, 952, 1439, 1634, 1831
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 1640, 1679
HpyF3I CTNAG 5 cut(s) 470, 518, 720, 1575, 1659
HspAI GCGC 1 cut(s) 1725
Ksp22I TGATCA 3 cut(s) 508, 640, 981
KspI CCGCGG 1 cut(s) 1059
Kzo9I GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
LmnI GCTCC 3 cut(s) 832, 1291, 1591
Lsp1109I GCAGC 7 cut(s) 171, 199, 619, 1420, 1423, 1595, 1709
LweI GCATC 5 cut(s) 345, 814, 836, 1070, 1840
MaeI CTAG 7 cut(s) 411, 498, 735, 798, 816, 1103, 1332
MaeIII GTNAC 6 cut(s) 715, 998, 1142, 1212, 1326, 1742
MalI GATC 8 cut(s) 246, 348, 510, 619, 642, 699, 983, 1278
MboI GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
MflI RGATCY 2 cut(s) 346, 697
MhlI GDGCHC 2 cut(s) 935, 1799
MlsI TGGCCA 2 cut(s) 730, 1155
MluCI AATT 6 cut(s) 1189, 1252, 1301, 1546, 1570, 1787
MluNI TGGCCA 2 cut(s) 730, 1155
MmeI TCCRAC 1 cut(s) 854
Mox20I TGGCCA 2 cut(s) 730, 1155
Mph1103I ATGCAT 1 cut(s) 360
MroXI GAANNNNTTC 1 cut(s) 439
MscI TGGCCA 2 cut(s) 730, 1155
MseI TTAA 5 cut(s) 308, 836, 1422, 1515, 1752
MslI CAYNNNNRTG 3 cut(s) 165, 928, 1034
Msp20I TGGCCA 2 cut(s) 730, 1155
MspA1I CMGCKG 3 cut(s) 632, 778, 1058
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 2 cut(s) 1058, 1727
MwoI GCNNNNNNNGC 3 cut(s) 596, 1640, 1679
NcoI CCATGG 1 cut(s) 1156
NdeII GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
NlaIV GGNNCC 2 cut(s) 699, 1122
NmuCI GTSAC 2 cut(s) 1212, 1742
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 2 cut(s) 146, 973
OliI CACNNNNGTG 1 cut(s) 928
PaeR7I CTCGAG 2 cut(s) 487, 897
PagI TCATGA 4 cut(s) 160, 247, 505, 532
PciI ACATGT 2 cut(s) 142, 969
PdmI GAANNNNTTC 1 cut(s) 439
PfeI GAWTC 3 cut(s) 104, 260, 1820
PscI ACATGT 2 cut(s) 142, 969
Psp6I CCWGG 1 cut(s) 342
PspEI GGTNACC 2 cut(s) 998, 1326
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 699, 1122
PspPI GGNCC 4 cut(s) 610, 1120, 1160, 1307
PspXI VCTCGAGB 2 cut(s) 487, 897
PsrI GAACNNNNNNTAC 4 cut(s) 475, 507, 1759, 1791
PstI CTGCAG 2 cut(s) 189, 1441
PsuI RGATCY 2 cut(s) 346, 697
PvuII CAGCTG 2 cut(s) 632, 778
RsaI GTAC 7 cut(s) 418, 493, 502, 1237, 1348, 1705, 1717
RsaNI GTAC 7 cut(s) 417, 492, 501, 1236, 1347, 1704, 1716
RseI CAYNNNNRTG 3 cut(s) 165, 928, 1034
SacII CCGCGG 1 cut(s) 1059
SaqAI TTAA 5 cut(s) 308, 836, 1422, 1515, 1752
Sau3AI GATC 8 cut(s) 244, 346, 508, 617, 640, 697, 981, 1276
Sau96I GGNCC 4 cut(s) 610, 1120, 1160, 1307
ScaI AGTACT 4 cut(s) 418, 493, 502, 1717
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 2 cut(s) 935, 1799
SfaNI GCATC 5 cut(s) 345, 814, 836, 1070, 1840
SfcI CTRYAG 2 cut(s) 185, 1437
Sfr274I CTCGAG 2 cut(s) 487, 897
Sfr303I CCGCGG 1 cut(s) 1059
SgrBI CCGCGG 1 cut(s) 1059
SinI GGWCC 2 cut(s) 1160, 1307
SlaI CTCGAG 2 cut(s) 487, 897
SmiMI CAYNNNNRTG 3 cut(s) 165, 928, 1034
SmlI CTYRAG 3 cut(s) 487, 897, 1342
SmoI CTYRAG 3 cut(s) 487, 897, 1342
SpeI ACTAGT 1 cut(s) 410
Sse9I AATT 6 cut(s) 1189, 1252, 1301, 1546, 1570, 1787
SsiI CCGC 9 cut(s) 613, 962, 1053, 1056, 1058, 1503, 1679, 1682, 1727
SspMI CTAG 7 cut(s) 411, 498, 735, 798, 816, 1103, 1332
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 4 cut(s) 475, 600, 1156, 1331
TaaI ACNGT 7 cut(s) 403, 911, 919, 927, 1212, 1218, 1235
TaqI TCGA 5 cut(s) 325, 488, 898, 974, 1196
TasI AATT 6 cut(s) 1189, 1252, 1301, 1546, 1570, 1787
TatI WGTACW 6 cut(s) 416, 491, 500, 1235, 1346, 1715
TauI GCSGC 5 cut(s) 615, 1058, 1061, 1682, 1685
TfiI GAWTC 3 cut(s) 104, 260, 1820
Tru1I TTAA 5 cut(s) 308, 836, 1422, 1515, 1752
Tru9I TTAA 5 cut(s) 308, 836, 1422, 1515, 1752
TscAI CASTG 2 cut(s) 1288, 1636
TseFI GTSAC 2 cut(s) 1212, 1742
TseI GCWGC 7 cut(s) 184, 187, 632, 1433, 1436, 1583, 1722
Tsp45I GTSAC 2 cut(s) 1212, 1742
TspGWI ACGGA 1 cut(s) 1832
TspRI CASTG 2 cut(s) 1288, 1636
VpaK11BI GGWCC 2 cut(s) 1160, 1307
XapI RAATTY 2 cut(s) 1189, 1570
XceI RCATGY 2 cut(s) 146, 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XhoI CTCGAG 2 cut(s) 487, 897
XmaJI CCTAGG 1 cut(s) 1331
XmnI GAANNNNTTC 1 cut(s) 439
XspI CTAG 7 cut(s) 411, 498, 735, 798, 816, 1103, 1332
ZrmI AGTACT 4 cut(s) 418, 493, 502, 1717
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.