AT1G73440

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011149
Physical Location & Seq
Forward (+)
27611354 .. 27612815
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73440.1

Sequence Viewer

Length: 765 bp
ATGGCGAGGGGAGAATCGGAGGGAGAGAGCTCAGGAAGTGAACGAGAGAGTTCGAGCTCGAGTTCCGGCAACGAATCGGAGCCAACAAAGGGGACGATCTCGAAATATGAGAAGCAGAGGTTGTCAAGGATCGCTGAGAACAAAGCGAGATTGGATGCACTCGGCATCTCTAAAGCCGCCAAGGCTCTTCTGTCACCATCTCCAGTTTCGAAAAAACGTAGAGTTAAGCGGAATTCCGGTGAAGAAGACGATGATTATACACCCGTAATCGCCGACGGTGATGGTGATGAGGACGATGACGAAGTAGAAGAAATAGACGAAGACGAGGAGTTTCTGTGTAAAAGAAAGAACAAGTCCTCTGCATCCAAGAGAAAAGTCTCATCTAGGAAGATTTTGAATACATCAGTTTCCCTTGGTGAGGATGATGATGACTTAGACAAGGCAATAGCGCTTTCTCTACAGGGCTCTGTTGCAGGAAGCGATAAAGAGGCTGCAACTATGAAGAAGAAAAGACCGGAATTGATGAGCAAGACGCAGATGACACAGGATGAATTGGTCATGTACTTCTGTCAGTTTGATGAAGGTGGAAAAGGGTTCATTACTCTGAGAGATGTGGCGAAAATGGCAACTGTGCATGACTTCACATGGACAGAAGAGGAATTACAAGACATGATTCGTTGCTTTGACATGGACAAGGACGGAAAGCTAAGCTTGGATGAGTTCAGGAAGATTGTGTCGCGATGTCGCATGTTGAAAGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

254

Amino Acids

28.25

Weight (kDa)

4.98

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 184 - 245 5.1e-12 EF-hand domain pair
EF-hand_8 PF13833 216 - 243 2e-08 EF-hand domain pair
EF-hand_1 PF00036 220 - 247 1.5e-07 EF hand domain
EF-hand_5 PF13202 221 - 244 9.2e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 739
AciI CCGC 2 cut(s) 177, 229
AclWI GGATC 1 cut(s) 137
AcsI RAATTY 1 cut(s) 232
AfaI GTAC 1 cut(s) 563
AfeI AGCGCT 1 cut(s) 450
AfiI CCNNNNNNNGG 2 cut(s) 89, 418
AgsI TTSAA 2 cut(s) 397, 754
AluBI AGCT 4 cut(s) 30, 57, 706, 711
AluI AGCT 4 cut(s) 30, 57, 706, 711
Alw21I GWGCWC 2 cut(s) 32, 59
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 58
Aor51HI AGCGCT 1 cut(s) 450
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 232
AspLEI GCGC 1 cut(s) 451
AsuHPI GGTGA 5 cut(s) 186, 251, 290, 296, 428
AsuII TTCGAA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 58
BanII GRGCYC 3 cut(s) 32, 59, 467
BarI GAAGNNNNNNTAC 2 cut(s) 645, 677
BbsI GAAGAC 2 cut(s) 252, 327
Bbv12I GWGCWC 2 cut(s) 32, 59
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 205, 275
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 1 cut(s) 458
BfoI RGCGCY 1 cut(s) 452
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 2 cut(s) 177, 492
BlpI GCTNAGC 1 cut(s) 707
BlsI GCNGC 2 cut(s) 178, 493
BmeT110I CYCGRG 1 cut(s) 58
BmiI GGNNCC 1 cut(s) 81
BmsI GCATC 3 cut(s) 145, 174, 371
BpiI GAAGAC 2 cut(s) 252, 327
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 707
Bpu14I TTCGAA 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 180, 412
BsaWI WCCGGW 2 cut(s) 236, 514
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 418
Bse1I ACTGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 180, 412
BseGI GGATG 5 cut(s) 160, 362, 427, 553, 721
BseLI CCNNNNNNNGG 2 cut(s) 89, 418
BseMII CTCAG 3 cut(s) 45, 126, 596
BseNI ACTGG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 739
BsiHKAI GWGCWC 2 cut(s) 32, 59
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 3 cut(s) 66, 237, 515
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 2 cut(s) 89, 418
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 58
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 32, 59, 467
Bsp143I GATC 2 cut(s) 96, 129
Bsp1720I GCTNAGC 1 cut(s) 707
Bsp68I TCGCGA 1 cut(s) 739
BspACI CCGC 2 cut(s) 177, 229
BspCNI CTCAG 3 cut(s) 44, 127, 597
BspFNI CGCG 1 cut(s) 739
BspLI GGNNCC 1 cut(s) 81
BspPI GGATC 1 cut(s) 137
BspQI GCTCTTC 1 cut(s) 192
BspT104I TTCGAA 1 cut(s) 209
BsrI ACTGG 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 180, 412
BssMI GATC 2 cut(s) 96, 129
BssT1I CCWWGG 2 cut(s) 180, 412
Bst4CI ACNGT 2 cut(s) 278, 631
Bst6I CTCTTC 2 cut(s) 192, 648
BstBI TTCGAA 1 cut(s) 209
BstDEI CTNAG 5 cut(s) 31, 135, 433, 605, 707
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 5 cut(s) 160, 362, 427, 553, 721
BstFNI CGCG 1 cut(s) 739
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 2 cut(s) 99, 132
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 2 cut(s) 96, 129
BstMWI GCNNNNNNNGC 2 cut(s) 182, 623
BstNSI RCATGY 1 cut(s) 751
BstSFI CTRYAG 1 cut(s) 458
BstUI CGCG 1 cut(s) 739
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 2 cut(s) 252, 327
BtgZI GCGATG 1 cut(s) 754
BtsCI GGATG 5 cut(s) 160, 362, 427, 553, 721
BtuMI TCGCGA 1 cut(s) 739
CfoI GCGC 1 cut(s) 451
CseI GACGC 1 cut(s) 541
Csp6I GTAC 1 cut(s) 562
CviAII CATG 6 cut(s) 559, 635, 645, 670, 688, 748
CviJI RGCY 9 cut(s) 30, 57, 82, 176, 185, 465, 491, 706, 711
CviKI_1 RGCY 9 cut(s) 30, 57, 82, 176, 185, 465, 491, 706, 711
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 5 cut(s) 31, 135, 433, 605, 707
DpnI GATC 2 cut(s) 98, 131
DpnII GATC 2 cut(s) 96, 129
Eam1104I CTCTTC 2 cut(s) 192, 648
EarI CTCTTC 2 cut(s) 192, 648
Ecl136II GAGCTC 2 cut(s) 30, 57
Eco130I CCWWGG 2 cut(s) 180, 412
Eco24I GRGCYC 3 cut(s) 32, 59, 467
Eco47III AGCGCT 1 cut(s) 450
Eco53kI GAGCTC 2 cut(s) 30, 57
Eco88I CYCGRG 1 cut(s) 58
EcoICRI GAGCTC 2 cut(s) 30, 57
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 180, 412
EcoT38I GRGCYC 3 cut(s) 32, 59, 467
ErhI CCWWGG 2 cut(s) 180, 412
FaeI CATG 6 cut(s) 562, 638, 648, 673, 691, 751
FaiI YATR 9 cut(s) 108, 258, 500, 560, 636, 646, 671, 689, 749
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 1 cut(s) 106
FatI CATG 6 cut(s) 558, 634, 644, 669, 687, 747
Fnu4HI GCNGC 2 cut(s) 177, 492
FokI GGATG 5 cut(s) 167, 349, 434, 560, 728
FriOI GRGCYC 3 cut(s) 32, 59, 467
Fsp4HI GCNGC 2 cut(s) 177, 492
FspBI CTAG 1 cut(s) 384
GlaI GCGC 1 cut(s) 450
GluI GCNGC 2 cut(s) 177, 492
GsuI CTGGAG 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 452
HapII CCGG 3 cut(s) 66, 237, 515
HgaI GACGC 1 cut(s) 541
HhaI GCGC 1 cut(s) 451
Hin1II CATG 6 cut(s) 562, 638, 648, 673, 691, 751
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HindIII AAGCTT 1 cut(s) 709
HinfI GANTC 3 cut(s) 14, 74, 673
HpaII CCGG 3 cut(s) 66, 237, 515
HphI GGTGA 5 cut(s) 186, 251, 290, 296, 428
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 3 cut(s) 19, 79, 606
Hpy188III TCNNGA 5 cut(s) 33, 100, 724, 738, 762
Hpy8I GTNNAC 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 1 cut(s) 575
HpyCH4III ACNGT 2 cut(s) 278, 631
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 5 cut(s) 158, 362, 473, 494, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 623
HpyF3I CTNAG 5 cut(s) 31, 135, 433, 605, 707
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 6 cut(s) 562, 638, 648, 673, 691, 751
HspAI GCGC 1 cut(s) 449
Kzo9I GATC 2 cut(s) 96, 129
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 9 cut(s) 18, 79, 216, 250, 446, 459, 528, 530, 709
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 3 cut(s) 145, 174, 371
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 1 cut(s) 192
MalI GATC 2 cut(s) 98, 131
MboI GATC 2 cut(s) 96, 129
MhlI GDGCHC 3 cut(s) 32, 59, 467
MluCI AATT 4 cut(s) 232, 518, 551, 659
MnlI CCTC 8 cut(s) 13, 111, 283, 319, 367, 412, 481, 649
MseI TTAA 1 cut(s) 225
MspI CCGG 3 cut(s) 66, 237, 515
MvnI CGCG 1 cut(s) 739
MwoI GCNNNNNNNGC 2 cut(s) 182, 623
NdeII GATC 2 cut(s) 96, 129
NlaIII CATG 6 cut(s) 562, 638, 648, 673, 691, 751
NlaIV GGNNCC 1 cut(s) 81
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 1 cut(s) 192
NruI TCGCGA 1 cut(s) 739
NspI RCATGY 1 cut(s) 751
NspV TTCGAA 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 58
PciSI GCTCTTC 1 cut(s) 192
PfeI GAWTC 3 cut(s) 14, 74, 673
PkrI GCNGC 2 cut(s) 178, 493
Psp124BI GAGCTC 2 cut(s) 32, 59
PspN4I GGNNCC 1 cut(s) 81
PspXI VCTCGAGB 1 cut(s) 58
RruI TCGCGA 1 cut(s) 739
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
SacI GAGCTC 2 cut(s) 32, 59
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 177, 492
Sau3AI GATC 2 cut(s) 96, 129
SduI GDGCHC 3 cut(s) 32, 59, 467
SetI ASST 8 cut(s) 32, 59, 122, 220, 586, 708, 713, 760
SfaNI GCATC 3 cut(s) 145, 174, 371
SfcI CTRYAG 1 cut(s) 458
Sfr274I CTCGAG 1 cut(s) 58
SfuI TTCGAA 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 4 cut(s) 232, 518, 551, 659
SsiI CCGC 2 cut(s) 177, 229
SspMI CTAG 1 cut(s) 384
SstI GAGCTC 2 cut(s) 32, 59
StyI CCWWGG 2 cut(s) 180, 412
TaaI ACNGT 2 cut(s) 278, 631
TaiI ACGT 1 cut(s) 220
TaqI TCGA 4 cut(s) 53, 59, 101, 209
TasI AATT 4 cut(s) 232, 518, 551, 659
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 3 cut(s) 14, 74, 673
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseFI GTSAC 1 cut(s) 192
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 192
TspDTI ATGAA 4 cut(s) 515, 564, 586, 594
TspGWI ACGGA 1 cut(s) 714
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 1 cut(s) 232
XceI RCATGY 1 cut(s) 751
XhoI CTCGAG 1 cut(s) 58
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.