FvH4_7g11100

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0011149
Physical Location & Seq
Reverse (-)
10377439 .. 10380425
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11100.t1

Sequence Viewer

Length: 813 bp
ATGCCGAAACACTCTGACCCCTCAGACGAAGAAACCTCGAGCTCCGGCTCAGAGGAACAAAACCCACAAGCGGGTGGTGGCGCTGTTCCAGAGTACGAGAAGCAGAGGCAAGCCAGAATAGCACACAACAGGGCGAGAATGGAGGCTCTGGGTCTGCCCAAAATGGCCTCTTCTCTAATGGGTTCGGCCCAGAATGCGACGAAGAGGAGTAGGAAGGGGAAAGGCAAGGTCTTGGAGGAGGACGATGATGAGGAGTATAGGCCTGAAGAAGAGGGACATGGTTTTTCTAGTGAGGAGGAGGAGGCTGATGATGATGAGGATTATGAGGATGAGGAATTATCTAGTGCACGTAAGAAAAAGAATAAAGGTAGGAAACATAAAAATGCTCGTTCAAGAAGTAGTTTGAGCAACTCGGATTACCTGGATGATGATGAGGCGTTGAAGCAGGCAATTGCTCTTTCATTACAAGGTTCTGCTGTTTCAGATGTGGTAAAAGATACTGTTAATGTAGGAGAGAACCAAACACAGCAAGATTCAGTGAGGAAGAGGAAAAAACCGTCTGCTAGTCGGCTTCAGATGACAGAGGATGAGTTGGTTTTGCACTTCTTTCAGTTTGATGAGAACTGCAATGGAGGAATTTCTGCTAGAAATTTAGAAAAATTAGCTGTCGCTCATGATTTTATATGGACTGATAAGGAGGTGGCTGACATGATTCATCTCTTTGATAGTGATGGAGATGCAAAGTTAAGTCTTGAAGATTTTCGGAAGATTGTTGTTCGTTGCAACATGATAAAAGCACCTGAAAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

271

Amino Acids

30.16

Weight (kDa)

4.94

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 198 - 257 2.7e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 71
AcsI RAATTY 3 cut(s) 636, 649, 805
AcuI CTGAAG 2 cut(s) 285, 557
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 70, 71
AgsI TTSAA 3 cut(s) 393, 442, 755
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 2 cut(s) 42, 665
AluI AGCT 2 cut(s) 42, 665
Alw21I GWGCWC 2 cut(s) 44, 349
Alw44I GTGCAC 1 cut(s) 345
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 3 cut(s) 165, 186, 260
ApaLI GTGCAC 1 cut(s) 345
ApoI RAATTY 3 cut(s) 636, 649, 805
Asp700I GAANNNNTTC 1 cut(s) 759
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 2 cut(s) 44, 349
BccI CCATC 1 cut(s) 725
BciT130I CCWGG 1 cut(s) 422
BfaI CTAG 4 cut(s) 288, 342, 564, 645
BfoI RGCGCY 1 cut(s) 84
Bme1390I CCNGG 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 727
BsaAI YACGTR 1 cut(s) 350
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 71
Bse3DI GCAATG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 422
BseGI GGATG 3 cut(s) 334, 430, 592
BseLI CCNNNNNNNGG 2 cut(s) 70, 71
BseMI GCAATG 1 cut(s) 634
BseMII CTCAG 2 cut(s) 36, 63
BseRI GAGGAG 6 cut(s) 220, 251, 266, 308, 311, 314
BseSI GKGCMC 1 cut(s) 349
BshFI GGCC 3 cut(s) 167, 188, 262
BsiHKAI GWGCWC 2 cut(s) 44, 349
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 70, 71
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 3 cut(s) 167, 188, 262
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 44, 349
BspACI CCGC 1 cut(s) 71
BspANI GGCC 3 cut(s) 167, 188, 262
BspCNI CTCAG 2 cut(s) 35, 62
BspHI TCATGA 2 cut(s) 673, 809
BsrDI GCAATG 1 cut(s) 634
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 502, 558
Bst6I CTCTTC 4 cut(s) 175, 197, 264, 539
BstBAI YACGTR 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 111, 447
BstDEI CTNAG 2 cut(s) 22, 49
BstF5I GGATG 3 cut(s) 334, 430, 592
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstMWI GCNNNNNNNGC 2 cut(s) 119, 194
BstNI CCWGG 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 349
BsuRI GGCC 3 cut(s) 167, 188, 262
BtsCI GGATG 3 cut(s) 334, 430, 592
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 2 cut(s) 111, 447
CciI TCATGA 2 cut(s) 673, 809
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 94
CviAII CATG 5 cut(s) 278, 674, 709, 787, 810
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 2 cut(s) 22, 49
Eam1104I CTCTTC 4 cut(s) 175, 197, 264, 539
EarI CTCTTC 4 cut(s) 175, 197, 264, 539
Ecl136II GAGCTC 1 cut(s) 42
Eco147I AGGCCT 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 285, 557
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 44
FaeI CATG 5 cut(s) 281, 677, 712, 790, 813
FaqI GGGAC 1 cut(s) 288
FatI CATG 5 cut(s) 277, 673, 708, 786, 809
FauI CCCGC 1 cut(s) 64
FokI GGATG 3 cut(s) 341, 437, 599
FriOI GRGCYC 1 cut(s) 44
FspBI CTAG 4 cut(s) 288, 342, 564, 645
GlaI GCGC 1 cut(s) 82
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 3 cut(s) 167, 188, 262
HapII CCGG 1 cut(s) 45
HhaI GCGC 1 cut(s) 83
Hin1II CATG 5 cut(s) 281, 677, 712, 790, 813
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 2 cut(s) 533, 712
HpaII CCGG 1 cut(s) 45
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 7 cut(s) 16, 25, 52, 415, 484, 576, 765
Hpy188III TCNNGA 5 cut(s) 89, 393, 674, 752, 810
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 502, 558
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 5 cut(s) 347, 601, 627, 740, 783
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 194
HpyF3I CTNAG 2 cut(s) 22, 49
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 5 cut(s) 281, 677, 712, 790, 813
HspAI GCGC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 47
LweI GCATC 1 cut(s) 727
MaeI CTAG 4 cut(s) 288, 342, 564, 645
MaeII ACGT 1 cut(s) 349
MboII GAAGA 8 cut(s) 41, 162, 214, 278, 281, 556, 767, 778
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 44, 349
MluCI AATT 6 cut(s) 335, 450, 636, 649, 659, 805
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 2 cut(s) 504, 746
MslI CAYNNNNRTG 1 cut(s) 381
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 2 cut(s) 119, 194
NlaIII CATG 5 cut(s) 281, 677, 712, 790, 813
PaeR7I CTCGAG 1 cut(s) 37
PagI TCATGA 2 cut(s) 673, 809
PceI AGGCCT 1 cut(s) 262
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 533, 712
Ppu21I YACGTR 1 cut(s) 350
Psp124BI GAGCTC 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspPI GGNCC 1 cut(s) 187
PspXI VCTCGAGB 1 cut(s) 37
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 44
SaqAI TTAA 2 cut(s) 504, 746
Sau96I GGNCC 1 cut(s) 187
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 2 cut(s) 44, 349
SfaNI GCATC 1 cut(s) 727
Sfr274I CTCGAG 1 cut(s) 37
SlaI CTCGAG 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 6 cut(s) 335, 450, 636, 649, 659, 805
SseBI AGGCCT 1 cut(s) 262
SsiI CCGC 1 cut(s) 71
SspMI CTAG 4 cut(s) 288, 342, 564, 645
SstI GAGCTC 1 cut(s) 44
StuI AGGCCT 1 cut(s) 262
StyD4I CCNGG 1 cut(s) 420
TaaI ACNGT 2 cut(s) 502, 558
TaiI ACGT 1 cut(s) 352
TaqI TCGA 1 cut(s) 38
TasI AATT 6 cut(s) 335, 450, 636, 649, 659, 805
TfiI GAWTC 2 cut(s) 533, 712
Tru1I TTAA 2 cut(s) 504, 746
Tru9I TTAA 2 cut(s) 504, 746
TscAI CASTG 1 cut(s) 543
TspDTI ATGAA 3 cut(s) 450, 704, 798
TspRI CASTG 1 cut(s) 543
VneI GTGCAC 1 cut(s) 345
XapI RAATTY 3 cut(s) 636, 649, 805
XhoI CTCGAG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 4 cut(s) 288, 342, 564, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.