Rh1AG218000

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0011149
Physical Location & Seq
Reverse (-)
41472686 .. 41475064
2379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG218000.1

Sequence Viewer

Length: 810 bp
ATGCCCAAACACTCTGATCCCTCAGACGAAGAAACCCCCGAGGACTCGAGCTCAGGCTCAGAGGAACAAAACCCACAAGATGGTGGTGTTCCAGAGTACGAGAAGCAGAGGCAAGCGAGAATTGCACACAACAGGGCCAGAATGGAGGCTCTGGGTCTGCCCAAAATCGCTTCCTCTCTAATGGGTTCGGCCCAGAATGCGACGAAGAGGAAGAGGAAGGGAAAAGCCAAGGTCTTGGAGGAGGACGACGACGATGAGTATAAGCCTGAAGAGGCTTCTTCTAGTGAAGACGAAGCTAATGATGAGGATTTTGAGGATGAAAAATCATCTAGTGCACGTAAGAAAAAGAATAAAGGTTCAAAACCTAAAAAGGCTCGTTCAAGAAGAAGTAGTTTGAGCAACTCGGATTACCTTGATGATGATGAGGCATTGAAGCAAGCAATTGCTCTTTCATTGCAAGATTCTGCTGTTTCAGGTGTGGTAAAAGCTAATGTTAATGAAAGAAAAGGAGAGAATCAAATTCAGCAAGATTCAGGGAGGAAGAGGAAAAACTCGTTTGCTAGTCGGCTGCAGATGACAGAGGATGAGTTTGTTTTGCACTTCTTTCAGTTTGATGAGAAATGCAATGGGGGAATTTCTGCTAGAGATTTAGAAAGAGTAGCTATTGCTCATGATTTTATGTGGACTGATAAGGAGGTGGCTGACATGATTCATCTCTTTGATAGTGATGGAGATGCAAAGCTAAGTCTTGAAGATTTTCGGAAGATTGTTGTTCGCTGCAACATGATAAAAGCACCTGAAAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

269

Amino Acids

30.19

Weight (kDa)

5.16

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 201 - 256 7e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 80
AclWI GGATC 1 cut(s) 11
AcsI RAATTY 3 cut(s) 519, 633, 802
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 4 cut(s) 360, 381, 433, 752
AluBI AGCT 5 cut(s) 51, 296, 488, 662, 742
AluI AGCT 5 cut(s) 51, 296, 488, 662, 742
Alw21I GWGCWC 2 cut(s) 53, 337
Alw44I GTGCAC 1 cut(s) 333
AlwI GGATC 1 cut(s) 11
Ama87I CYCGRG 2 cut(s) 38, 46
AoxI GGCC 2 cut(s) 135, 189
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 568, 777
ApoI RAATTY 3 cut(s) 519, 633, 802
Asp700I GAANNNNTTC 1 cut(s) 756
AspS9I GGNCC 2 cut(s) 135, 190
AvaI CYCGRG 2 cut(s) 38, 46
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 2 cut(s) 53, 337
BbvI GCAGC 2 cut(s) 555, 764
BccI CCATC 2 cut(s) 74, 722
BfaI CTAG 4 cut(s) 282, 330, 561, 642
BfmI CTRYAG 1 cut(s) 569
BisI GCNGC 2 cut(s) 569, 778
BlsI GCNGC 2 cut(s) 570, 779
BmeT110I CYCGRG 2 cut(s) 38, 46
BmgT120I GGNCC 2 cut(s) 135, 190
BmsI GCATC 1 cut(s) 724
BpiI GAAGAC 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 39, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse3DI GCAATG 2 cut(s) 452, 631
BseDI CCNNGG 2 cut(s) 39, 228
BseGI GGATG 2 cut(s) 322, 589
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 2 cut(s) 452, 631
BseMII CTCAG 3 cut(s) 36, 66, 72
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 2 cut(s) 555, 764
BshFI GGCC 2 cut(s) 137, 191
BsiHKAI GWGCWC 2 cut(s) 53, 337
BsiHKCI CYCGRG 2 cut(s) 38, 46
BslI CCNNNNNNNGG 1 cut(s) 80
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 2 cut(s) 137, 191
BsoBI CYCGRG 2 cut(s) 38, 46
Bsp1286I GDGCHC 2 cut(s) 53, 337
Bsp143I GATC 1 cut(s) 16
BspANI GGCC 2 cut(s) 137, 191
BspCNI CTCAG 3 cut(s) 35, 65, 71
BspHI TCATGA 2 cut(s) 670, 806
BspMAI CTGCAG 1 cut(s) 573
BspPI GGATC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 452, 631
BssECI CCNNGG 2 cut(s) 39, 228
BssMI GATC 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 228
Bst6I CTCTTC 4 cut(s) 200, 206, 264, 536
BstBAI YACGTR 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 114, 438
BstDEI CTNAG 4 cut(s) 22, 52, 58, 743
BstF5I GGATG 2 cut(s) 322, 589
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 122, 197
BstSFI CTRYAG 1 cut(s) 569
BstSLI GKGCMC 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 555, 764
BstV2I GAAGAC 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 2 cut(s) 137, 191
BtsCI GGATG 2 cut(s) 322, 589
Cac8I GCNNGC 2 cut(s) 114, 438
CciI TCATGA 2 cut(s) 670, 806
Cfr13I GGNCC 2 cut(s) 135, 190
Csp6I GTAC 1 cut(s) 97
CviAII CATG 4 cut(s) 671, 706, 784, 807
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 4 cut(s) 22, 52, 58, 743
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
Eam1104I CTCTTC 4 cut(s) 200, 206, 264, 536
EarI CTCTTC 4 cut(s) 200, 206, 264, 536
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 38, 46
EcoICRI GAGCTC 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 4 cut(s) 674, 709, 787, 810
FaiI YATR 6 cut(s) 261, 672, 680, 707, 785, 808
FatI CATG 4 cut(s) 670, 705, 783, 806
Fnu4HI GCNGC 2 cut(s) 569, 778
FokI GGATG 2 cut(s) 329, 596
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 569, 778
FspBI CTAG 4 cut(s) 282, 330, 561, 642
GluI GCNGC 2 cut(s) 569, 778
HaeIII GGCC 2 cut(s) 137, 191
Hin1II CATG 4 cut(s) 674, 709, 787, 810
HinfI GANTC 5 cut(s) 44, 461, 514, 530, 709
Hpy166II GTNNAC 2 cut(s) 335, 684
Hpy188I TCNGA 5 cut(s) 16, 25, 61, 406, 762
Hpy188III TCNNGA 5 cut(s) 92, 381, 671, 749, 807
Hpy8I GTNNAC 2 cut(s) 335, 684
Hpy99I CGWCG 3 cut(s) 205, 251, 254
HpyAV CCTTC 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 8 cut(s) 125, 335, 457, 571, 598, 624, 737, 780
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 197
HpyF3I CTNAG 4 cut(s) 22, 52, 58, 743
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 4 cut(s) 674, 709, 787, 810
Kzo9I GATC 1 cut(s) 16
LpnPI CCDG 9 cut(s) 39, 105, 118, 137, 151, 206, 279, 459, 519
Lsp1109I GCAGC 2 cut(s) 555, 764
LweI GCATC 1 cut(s) 724
MaeI CTAG 4 cut(s) 282, 330, 561, 642
MaeII ACGT 1 cut(s) 337
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 2 cut(s) 53, 337
MluCI AATT 5 cut(s) 120, 441, 519, 633, 802
MlyI GAGTC 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 756
MseI TTAA 1 cut(s) 495
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 202
MwoI GCNNNNNNNGC 2 cut(s) 122, 197
NdeII GATC 1 cut(s) 16
NlaIII CATG 4 cut(s) 674, 709, 787, 810
PaeR7I CTCGAG 1 cut(s) 46
PagI TCATGA 2 cut(s) 670, 806
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 4 cut(s) 461, 514, 530, 709
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 2 cut(s) 570, 779
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Ppu21I YACGTR 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 53
PspPI GGNCC 2 cut(s) 135, 190
PspXI VCTCGAGB 1 cut(s) 46
PstI CTGCAG 1 cut(s) 573
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 2 cut(s) 569, 778
Sau3AI GATC 1 cut(s) 16
Sau96I GGNCC 2 cut(s) 135, 190
SchI GAGTC 1 cut(s) 38
SduI GDGCHC 2 cut(s) 53, 337
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 569
Sfr274I CTCGAG 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 5 cut(s) 120, 441, 519, 633, 802
SspMI CTAG 4 cut(s) 282, 330, 561, 642
SstI GAGCTC 1 cut(s) 53
StyI CCWWGG 1 cut(s) 228
TaiI ACGT 1 cut(s) 340
TaqI TCGA 1 cut(s) 47
TasI AATT 5 cut(s) 120, 441, 519, 633, 802
TfiI GAWTC 4 cut(s) 461, 514, 530, 709
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseI GCWGC 2 cut(s) 568, 777
TspDTI ATGAA 5 cut(s) 333, 441, 513, 701, 795
Van91I CCANNNNNTGG 1 cut(s) 80
VneI GTGCAC 1 cut(s) 333
XapI RAATTY 3 cut(s) 519, 633, 802
XhoI CTCGAG 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 4 cut(s) 282, 330, 561, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.