RLG00000028604

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0011149
Physical Location & Seq
Forward (+)
24950913 .. 24953367
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028604

Sequence Viewer

Length: 819 bp
ATGCCCAAACACTCTGATCCCTCAGACGAAGAAACCCCCGAGGACTCGAGCTTAGGCTCAGAGGAACAGAACCCACAAGATGGTGGTGTTCCAGAGTACGAGAAGCAGAGGCAAGCGAGAATTGCACACAACAGGGCCAGAATGGAGGCTCTGGGTCTGCCCAAGATCGCTTCCTCTCTAATGGGTTCGGCCCAGAATGCGACGAAGAGGAAGAGAAAGGGAAAAGCCAAGATCTTGGAGGAGGACGATGATGATGAGTATAGGCCTGAAGAGGAGGGACATGGTTCTTCTAGCGAAGAGGAAGCTAATGATGAGGATTATGAGGATGAAAAATCACCTGGTGCACGTAAGAAAAAGAATAAAGGTTCAAAACCTAAAAAGGCTGCTTCAAGAAGAAGTAGTTTGAGCAACTTGGATTACCTTGATGATGATGAGGCATTGAAGCAGGCAATTGCTCTTTCATTACAAGATTCTGCTGTTTCAGGTGTGGTAAAAGCTAATGTTAATGAAAGGAAAGGAGAGAATCAAATTCAGCAAGATTCAGGGAGGAAGAGGAAAAACTCGTTTTCTAGTCGGCTGCAGATGACAGAGGATGAGTTGGTTCTGCACTTCTTTCAGTTTGATGAGAGATGCAATGGGGGAATTTCTGCTAGAGATTTAGAAAGAGTAGCTATTGCTCATGATTTTATGTGGACTGATAAGGAGGTGGCTGACATGATTCATCTCTTTGATAGTGATGGAGATGCAAAGCTAAGTCTTGAAGATTTTCGGAAGATTGTTGTTCGCTGCAACATGATAAAAGCACCTGAAAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

273

Amino Acids

30.53

Weight (kDa)

5.1

Isoelectric Point (pI)

53.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 200 - 259 4e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 80
AclWI GGATC 1 cut(s) 11
AcsI RAATTY 3 cut(s) 528, 642, 811
AcuI CTGAAG 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 4 cut(s) 369, 390, 442, 761
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 5 cut(s) 51, 305, 497, 671, 751
AluI AGCT 5 cut(s) 51, 305, 497, 671, 751
Alw21I GWGCWC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 11
Ama87I CYCGRG 2 cut(s) 38, 46
AoxI GGCC 3 cut(s) 135, 189, 263
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 383, 577, 786
ApoI RAATTY 3 cut(s) 528, 642, 811
Asp700I GAANNNNTTC 1 cut(s) 765
AspS9I GGNCC 2 cut(s) 135, 190
AsuHPI GGTGA 1 cut(s) 327
AvaI CYCGRG 2 cut(s) 38, 46
BaeGI GKGCMC 1 cut(s) 346
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 3 cut(s) 370, 564, 773
BccI CCATC 2 cut(s) 74, 731
BciT130I CCWGG 1 cut(s) 339
BfaI CTAG 3 cut(s) 291, 570, 651
BfmI CTRYAG 1 cut(s) 578
BglII AGATCT 1 cut(s) 231
BisI GCNGC 3 cut(s) 384, 578, 787
BlsI GCNGC 3 cut(s) 385, 579, 788
Bme1390I CCNGG 1 cut(s) 339
BmeT110I CYCGRG 2 cut(s) 38, 46
BmgT120I GGNCC 2 cut(s) 135, 190
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 620, 733
Bpu10I CCTNAGC 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 640
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 331, 598
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 2 cut(s) 36, 72
BseRI GAGGAG 2 cut(s) 254, 287
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 3 cut(s) 370, 564, 773
BsgI GTGCAG 1 cut(s) 590
BshFI GGCC 3 cut(s) 137, 191, 265
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 2 cut(s) 38, 46
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 3 cut(s) 137, 191, 265
BsoBI CYCGRG 2 cut(s) 38, 46
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 3 cut(s) 16, 165, 231
BspANI GGCC 3 cut(s) 137, 191, 265
BspCNI CTCAG 2 cut(s) 35, 71
BspHI TCATGA 2 cut(s) 679, 815
BspMAI CTGCAG 1 cut(s) 582
BspPI GGATC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 640
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 3 cut(s) 16, 165, 231
Bst2UI CCWGG 1 cut(s) 339
Bst6I CTCTTC 5 cut(s) 200, 206, 264, 291, 545
BstBAI YACGTR 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 114, 447
BstDEI CTNAG 4 cut(s) 22, 52, 58, 752
BstF5I GGATG 2 cut(s) 331, 598
BstKTI GATC 3 cut(s) 19, 168, 234
BstMBI GATC 3 cut(s) 16, 165, 231
BstMWI GCNNNNNNNGC 2 cut(s) 122, 197
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 578
BstSLI GKGCMC 1 cut(s) 346
BstV1I GCAGC 3 cut(s) 370, 564, 773
BstX2I RGATCY 1 cut(s) 231
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 3 cut(s) 137, 191, 265
BtsCI GGATG 2 cut(s) 331, 598
Cac8I GCNNGC 2 cut(s) 114, 447
CciI TCATGA 2 cut(s) 679, 815
Cfr13I GGNCC 2 cut(s) 135, 190
CsiI ACCWGGT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 97
CviAII CATG 5 cut(s) 281, 680, 715, 793, 816
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 4 cut(s) 22, 52, 58, 752
DpnI GATC 3 cut(s) 18, 167, 233
DpnII GATC 3 cut(s) 16, 165, 231
DraIII CACNNNGTG 1 cut(s) 341
Eam1104I CTCTTC 5 cut(s) 200, 206, 264, 291, 545
EarI CTCTTC 5 cut(s) 200, 206, 264, 291, 545
Eco147I AGGCCT 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 38, 46
EcoRII CCWGG 1 cut(s) 337
FaeI CATG 5 cut(s) 284, 683, 718, 796, 819
FaiI YATR 8 cut(s) 261, 282, 321, 681, 689, 716, 794, 817
FaqI GGGAC 1 cut(s) 291
FatI CATG 5 cut(s) 280, 679, 714, 792, 815
Fnu4HI GCNGC 3 cut(s) 384, 578, 787
FokI GGATG 2 cut(s) 338, 605
Fsp4HI GCNGC 3 cut(s) 384, 578, 787
FspBI CTAG 3 cut(s) 291, 570, 651
GluI GCNGC 3 cut(s) 384, 578, 787
HaeIII GGCC 3 cut(s) 137, 191, 265
Hin1II CATG 5 cut(s) 284, 683, 718, 796, 819
HinfI GANTC 5 cut(s) 44, 470, 523, 539, 718
HphI GGTGA 1 cut(s) 327
Hpy166II GTNNAC 2 cut(s) 344, 693
Hpy188I TCNGA 4 cut(s) 16, 25, 61, 771
Hpy188III TCNNGA 5 cut(s) 92, 390, 680, 758, 816
Hpy8I GTNNAC 2 cut(s) 344, 693
Hpy99I CGWCG 1 cut(s) 205
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 7 cut(s) 125, 344, 580, 607, 633, 746, 789
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 197
HpyF3I CTNAG 4 cut(s) 22, 52, 58, 752
HpySE526I ACGT 1 cut(s) 346
Hsp92II CATG 5 cut(s) 284, 683, 718, 796, 819
Kzo9I GATC 3 cut(s) 16, 165, 231
Lsp1109I GCAGC 3 cut(s) 370, 564, 773
LweI GCATC 2 cut(s) 620, 733
MabI ACCWGGT 1 cut(s) 337
MaeI CTAG 3 cut(s) 291, 570, 651
MaeII ACGT 1 cut(s) 346
MalI GATC 3 cut(s) 18, 167, 233
MboI GATC 3 cut(s) 16, 165, 231
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 5 cut(s) 120, 450, 528, 642, 811
MlyI GAGTC 1 cut(s) 38
MroXI GAANNNNTTC 1 cut(s) 765
MseI TTAA 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 339
MunI CAATTG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 122, 197
NdeII GATC 3 cut(s) 16, 165, 231
NlaIII CATG 5 cut(s) 284, 683, 718, 796, 819
PaeR7I CTCGAG 1 cut(s) 46
PagI TCATGA 2 cut(s) 679, 815
PceI AGGCCT 1 cut(s) 265
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 4 cut(s) 470, 523, 539, 718
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 3 cut(s) 385, 579, 788
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Ppu21I YACGTR 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspPI GGNCC 2 cut(s) 135, 190
PspXI VCTCGAGB 1 cut(s) 46
PstI CTGCAG 1 cut(s) 582
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 3 cut(s) 384, 578, 787
Sau3AI GATC 3 cut(s) 16, 165, 231
Sau96I GGNCC 2 cut(s) 135, 190
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 346
SexAI ACCWGGT 1 cut(s) 337
SfaNI GCATC 2 cut(s) 620, 733
SfcI CTRYAG 1 cut(s) 578
Sfr274I CTCGAG 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 5 cut(s) 120, 450, 528, 642, 811
SseBI AGGCCT 1 cut(s) 265
SspMI CTAG 3 cut(s) 291, 570, 651
StuI AGGCCT 1 cut(s) 265
StyD4I CCNGG 1 cut(s) 337
TaiI ACGT 1 cut(s) 349
TaqI TCGA 1 cut(s) 47
TasI AATT 5 cut(s) 120, 450, 528, 642, 811
TfiI GAWTC 4 cut(s) 470, 523, 539, 718
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TseI GCWGC 3 cut(s) 383, 577, 786
TspDTI ATGAA 5 cut(s) 342, 450, 522, 710, 804
Van91I CCANNNNNTGG 1 cut(s) 80
VneI GTGCAC 1 cut(s) 342
XapI RAATTY 3 cut(s) 528, 642, 811
XhoI CTCGAG 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 3 cut(s) 291, 570, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.