MD02G1210400.v1.1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011149
Physical Location & Seq
Forward (+)
21931907 .. 21934711
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1210400.v1.1.491

Sequence Viewer

Length: 864 bp
ATGCCGAAACGCCATCTCTCCTACTCAGACGTCGAAGAAACCTCCGAGAACTCCAGCGCAGAGTCGGAGGACGCGAGAAAACCCATCAATGGCGGGAAAAACCCAGCGGGAGCAGTGTCGGAGTACGAGAAGCAGAGGCTATCGAGAATTGCAGAGAACAGGGCGAGAATGGAGGCTCTGGGTCTGCCTAAAATCGCTTCTTCTTTAATGGGTTCGGGCCCGAATGCGAAGAAGAAGAAGGAGCAGAAGAAGGGGAAAGCCAAGGTCTTGGAGGACGACGAGGACTATAGGCCTGACGAAGACCACGAGGGACCCAGTTCGTCTGGTAGCCGAGAGGAAGAAGCCATGGAGGAGGACGATGACGATGATTTTGTGACAGTAAAAACAAAATCTGGTTCGCGGGGAAAAAAGAATAAAAGTTCAAAATCTAAGAAGGTTCTTTCCCGAAGCACTTTGAGTAACGATGACTGCATCGATGATGATGAAGCATTGAAGCATGCAATTTCTCTTTCATTACAAGGCTCTGATGTGAAAAATGCTGATGTTACGGGAAGAAAGGGAAACCCTCAGATACAGGAAGATACAGGAAGGAGGAAGAGGAAAAATTCGTTTGCTAGTCGGCTCCAGATGACAGAGGATGAGTTGATTTTGCACTTCTTTCAGTTTGATGAGAAATGTGATGGGGGAATCTCTATAGCAGATATACAAAGATTAGCAACTACCCATGATTTTATGTGGAATAAGGAGGAATTGGCGGACATGATTCACTGTTTTGATAGTGATGGCGATGGAAAGTTGAGTCTGGAGGAGTTTCGAAAGATTGTTGTTCGTTGCAACATGATCAAAGAGCCCGAGCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

288

Amino Acids

32.11

Weight (kDa)

5.32

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 213 - 274 1.3e-10 EF-hand domain pair
EF-hand_8 PF13833 228 - 277 7e-07 EF-hand domain pair
EF-hand_1 PF00036 250 - 275 1.4e-06 EF hand domain
EF-hand_5 PF13202 251 - 274 5e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
AccII CGCG 2 cut(s) 74, 400
AciI CCGC 4 cut(s) 93, 107, 400, 755
AcsI RAATTY 1 cut(s) 604
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 89
AgsI TTSAA 2 cut(s) 423, 493
Ama87I CYCGRG 1 cut(s) 851
AoxI GGCC 2 cut(s) 217, 290
ApaI GGGCCC 1 cut(s) 221
ApoI RAATTY 1 cut(s) 604
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 3 cut(s) 217, 218, 311
AsuII TTCGAA 1 cut(s) 814
AvaI CYCGRG 1 cut(s) 851
AvaII GGWCC 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 221
BanII GRGCYC 2 cut(s) 221, 852
BauI CACGAG 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 306
BccI CCATC 5 cut(s) 21, 92, 674, 776, 782
BclI TGATCA 1 cut(s) 840
BfaI CTAG 1 cut(s) 615
BfmI CTRYAG 2 cut(s) 286, 693
Bme18I GGWCC 1 cut(s) 311
BmeT110I CYCGRG 1 cut(s) 851
BmgT120I GGNCC 3 cut(s) 217, 218, 311
BmiI GGNNCC 4 cut(s) 219, 312, 313, 623
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 480
BmuI ACTGGG 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 3 cut(s) 37, 608, 824
Bpu14I TTCGAA 1 cut(s) 814
Bsa29I ATCGAT 1 cut(s) 474
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 261, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 315
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 261, 345
BseGI GGATG 1 cut(s) 643
BseLI CCNNNNNNNGG 1 cut(s) 89
BseMII CTCAG 2 cut(s) 39, 581
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 2 cut(s) 365, 821
BseSI GKGCMC 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 103
Bsh1236I CGCG 2 cut(s) 74, 400
BshFI GGCC 2 cut(s) 219, 292
BshVI ATCGAT 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 851
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 2 cut(s) 219, 292
BsoBI CYCGRG 1 cut(s) 851
Bsp119I TTCGAA 1 cut(s) 814
Bsp120I GGGCCC 1 cut(s) 217
Bsp1286I GDGCHC 2 cut(s) 221, 852
Bsp143I GATC 1 cut(s) 840
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 4 cut(s) 93, 107, 400, 755
BspANI GGCC 2 cut(s) 219, 292
BspCNI CTCAG 2 cut(s) 38, 580
BspDI ATCGAT 1 cut(s) 474
BspFNI CGCG 2 cut(s) 74, 400
BspLI GGNNCC 4 cut(s) 219, 312, 313, 623
BspT104I TTCGAA 1 cut(s) 814
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 2 cut(s) 261, 345
BssMI GATC 1 cut(s) 840
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 305
BssT1I CCWWGG 2 cut(s) 261, 345
Bst2BI CACGAG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 379, 770
Bst6I CTCTTC 1 cut(s) 590
BstACI GRCGYC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 814
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 3 cut(s) 25, 429, 567
BstDSI CCRYGG 1 cut(s) 345
BstF5I GGATG 1 cut(s) 643
BstFNI CGCG 2 cut(s) 74, 400
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 1 cut(s) 843
BstMBI GATC 1 cut(s) 840
BstNSI RCATGY 1 cut(s) 500
BstSFI CTRYAG 2 cut(s) 286, 693
BstSLI GKGCMC 1 cut(s) 221
BstUI CGCG 2 cut(s) 74, 400
BstV2I GAAGAC 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 268
Bsu15I ATCGAT 1 cut(s) 474
BsuRI GGCC 2 cut(s) 219, 292
BsuTUI ATCGAT 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 345
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 1 cut(s) 643
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 120, 766
Cac8I GCNNGC 1 cut(s) 498
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 3 cut(s) 217, 218, 311
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 1 cut(s) 80
Csp6I GTAC 1 cut(s) 124
CviAII CATG 5 cut(s) 346, 497, 725, 760, 838
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 3 cut(s) 25, 429, 567
DpnI GATC 1 cut(s) 842
DpnII GATC 1 cut(s) 840
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
EciI GGCGGA 1 cut(s) 770
Eco130I CCWWGG 2 cut(s) 261, 345
Eco147I AGGCCT 1 cut(s) 292
Eco24I GRGCYC 2 cut(s) 221, 852
Eco47I GGWCC 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 311
EcoT14I CCWWGG 2 cut(s) 261, 345
EcoT38I GRGCYC 2 cut(s) 221, 852
ErhI CCWWGG 2 cut(s) 261, 345
FaeI CATG 5 cut(s) 349, 500, 728, 763, 841
FaiI YATR 9 cut(s) 288, 347, 498, 695, 704, 726, 734, 761, 839
FaqI GGGAC 1 cut(s) 324
FatI CATG 5 cut(s) 345, 496, 724, 759, 837
FauI CCCGC 3 cut(s) 86, 100, 393
FbaI TGATCA 1 cut(s) 840
FokI GGATG 1 cut(s) 650
FriOI GRGCYC 2 cut(s) 221, 852
FspBI CTAG 1 cut(s) 615
GlaI GCGC 1 cut(s) 58
GsaI CCCAGC 1 cut(s) 107
GsuI CTGGAG 3 cut(s) 37, 608, 824
HaeIII GGCC 2 cut(s) 219, 292
HgaI GACGC 1 cut(s) 80
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 5 cut(s) 349, 500, 728, 763, 841
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HinfI GANTC 4 cut(s) 62, 687, 763, 799
Hpy188I TCNGA 6 cut(s) 28, 46, 67, 121, 526, 570
Hpy188III TCNNGA 5 cut(s) 144, 444, 625, 803, 861
Hpy99I CGWCG 2 cut(s) 35, 281
HpyAV CCTTC 4 cut(s) 232, 244, 427, 582
HpyCH4III ACNGT 2 cut(s) 379, 770
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 5 cut(s) 152, 471, 500, 652, 834
HpyF3I CTNAG 3 cut(s) 25, 429, 567
HpySE526I ACGT 1 cut(s) 30
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 5 cut(s) 349, 500, 728, 763, 841
HspAI GCGC 1 cut(s) 57
KflI GGGWCCC 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 1 cut(s) 840
LmnI GCTCC 3 cut(s) 110, 241, 627
LweI GCATC 1 cut(s) 480
MaeI CTAG 1 cut(s) 615
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 3 cut(s) 373, 458, 544
MalI GATC 1 cut(s) 842
MboI GATC 1 cut(s) 840
MhlI GDGCHC 2 cut(s) 221, 852
MluCI AATT 4 cut(s) 147, 501, 604, 749
MlyI GAGTC 2 cut(s) 71, 808
MmeI TCCRAC 2 cut(s) 45, 99
MseI TTAA 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 229
MvnI CGCG 2 cut(s) 74, 400
NcoI CCATGG 1 cut(s) 345
NdeII GATC 1 cut(s) 840
NlaIII CATG 5 cut(s) 349, 500, 728, 763, 841
NlaIV GGNNCC 4 cut(s) 219, 312, 313, 623
NmeAIII GCCGAG 1 cut(s) 356
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 1 cut(s) 500
NspV TTCGAA 1 cut(s) 814
PaeI GCATGC 1 cut(s) 500
PceI AGGCCT 1 cut(s) 292
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 303
PctI GAATGC 1 cut(s) 229
PfeI GAWTC 2 cut(s) 687, 763
PleI GAGTC 2 cut(s) 70, 807
PpsI GAGTC 2 cut(s) 70, 807
PpuMI RGGWCCY 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 311
PspFI CCCAGC 1 cut(s) 103
PspN4I GGNNCC 4 cut(s) 219, 312, 313, 623
PspOMI GGGCCC 1 cut(s) 217
PspPI GGNCC 3 cut(s) 217, 218, 311
PspPPI RGGWCCY 1 cut(s) 311
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 206
Sau3AI GATC 1 cut(s) 840
Sau96I GGNCC 3 cut(s) 217, 218, 311
SchI GAGTC 2 cut(s) 71, 808
SduI GDGCHC 2 cut(s) 221, 852
SetI ASST 4 cut(s) 33, 44, 267, 438
SfaNI GCATC 1 cut(s) 480
SfcI CTRYAG 2 cut(s) 286, 693
SfuI TTCGAA 1 cut(s) 814
SinI GGWCC 1 cut(s) 311
SphI GCATGC 1 cut(s) 500
Sse9I AATT 4 cut(s) 147, 501, 604, 749
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 4 cut(s) 93, 107, 400, 755
SspMI CTAG 1 cut(s) 615
StuI AGGCCT 1 cut(s) 292
StyI CCWWGG 2 cut(s) 261, 345
TaaI ACNGT 2 cut(s) 379, 770
TaiI ACGT 1 cut(s) 33
TaqI TCGA 4 cut(s) 33, 143, 474, 814
TasI AATT 4 cut(s) 147, 501, 604, 749
TfiI GAWTC 2 cut(s) 687, 763
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TscAI CASTG 2 cut(s) 120, 773
TseFI GTSAC 1 cut(s) 373
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 2 cut(s) 498, 501
TspRI CASTG 2 cut(s) 120, 773
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 500
XspI CTAG 1 cut(s) 615
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.