AT4G00140

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011149
Physical Location & Seq
Reverse (-)
51166 .. 53449
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00140.1

Sequence Viewer

Length: 774 bp
ATGGCGAGGGGAGAATCGGAGGGAGAGAGCTCAGGAAGTGAACGAGAGAGTTCGAGCTCGAGTTCCGGCAACGAATCGGAGCCGATAAAGGGGAAAATCTCGGAATATGAGAAGCAGAGGTTGTCAAGGATCGCTGAGAACAAAGCGAGATTGGATGCACTCGGAATTCCGGCAATAGCGCTTTCTCTACAGGGCTCTGTTGCAGGAGGCTCTCGTACGAAAAATACGAGAAGCGATAAAGAGGCTGCAACTATGAAGAAGAAAAGACAGGAAGGTGGAAAAGGGTTCATTACTCGGAGAGATGTGGCGAAAATGGCAACGGTGCATGACTTCACATGGACAGAAGAGGAATTACAAGACATGATTCGTTCCTTTGACATGGACAAGGACGGAAAGGTGGGTACTAAGTTGAAGGTATTCACGTTTATTAGAGAAACATTATTGCTAAACATCTTTAATTTTGGGCAGCTAAGCTGCCGATCGTCTGACGGAACGGGTCGGACAGACCGATCGTCGGATGGGATGGATCGAGAAAAGTGTTCGAGGAAGCACAAGGATAAGTGCATCCGAACACACCCAAGGGTCGCCCATGGATGCAATCTTACCGGTAAAGGGAGAAAGCACCAAGAATGGGTAAGGAACGTGATTCACCATATAGGATCAACATCGCGGCCGCCTTCATGTATCTATCTCCTCAGCCATGGTGAAACCGATCAAAGATCGGTAAGACCAAAGGGTGACTCGATGGATAACCAACGGGAAAGTCCACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

257

Amino Acids

28.69

Weight (kDa)

9.61

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 670
AciI CCGC 2 cut(s) 670, 674
AclWI GGATC 3 cut(s) 137, 534, 667
AcoI YGGCCR 1 cut(s) 671
AcsI RAATTY 1 cut(s) 165
AfaI GTAC 2 cut(s) 217, 403
AfeI AGCGCT 1 cut(s) 180
AfiI CCNNNNNNNGG 4 cut(s) 89, 514, 612, 631
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 1 cut(s) 412
AhdI GACNNNNNGTC 1 cut(s) 511
AluBI AGCT 4 cut(s) 30, 57, 469, 474
AluI AGCT 4 cut(s) 30, 57, 469, 474
Alw21I GWGCWC 2 cut(s) 32, 59
AlwI GGATC 3 cut(s) 137, 534, 667
Ama87I CYCGRG 1 cut(s) 58
Aor51HI AGCGCT 1 cut(s) 180
AoxI GGCC 1 cut(s) 671
ApeKI GCWGC 3 cut(s) 245, 466, 474
ApoI RAATTY 1 cut(s) 165
AsiGI ACCGGT 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 181
AsuHPI GGTGA 3 cut(s) 641, 716, 749
AvaI CYCGRG 1 cut(s) 58
BanII GRGCYC 3 cut(s) 32, 59, 197
BarI GAAGNNNNNNTAC 2 cut(s) 336, 368
Bbv12I GWGCWC 2 cut(s) 32, 59
BbvCI CCTCAGC 1 cut(s) 695
BbvI GCAGC 3 cut(s) 232, 461, 478
BccI CCATC 3 cut(s) 512, 517, 739
BfmI CTRYAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 5 cut(s) 246, 467, 475, 671, 674
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 5 cut(s) 247, 468, 476, 672, 675
BmeRI GACNNNNNGTC 1 cut(s) 511
BmeT110I CYCGRG 1 cut(s) 58
BmiI GGNNCC 1 cut(s) 81
BmsI GCATC 3 cut(s) 145, 573, 584
Bpu10I CCTNAGC 2 cut(s) 31, 695
Bpu1102I GCTNAGC 1 cut(s) 470
BsaBI GATNNNNATC 1 cut(s) 664
BsaJI CCNNGG 4 cut(s) 578, 589, 700, 767
BsaWI WCCGGW 1 cut(s) 605
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 514, 612, 631
Bse118I RCCGGY 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 664
BseDI CCNNGG 4 cut(s) 578, 589, 700, 767
BseGI GGATG 5 cut(s) 160, 523, 528, 564, 599
BseJI GATNNNNATC 1 cut(s) 664
BseLI CCNNNNNNNGG 4 cut(s) 89, 514, 612, 631
BseMII CTCAG 3 cut(s) 45, 126, 709
BseRI GAGGAG 1 cut(s) 683
BseX3I CGGCCG 1 cut(s) 671
BseXI GCAGC 3 cut(s) 232, 461, 478
Bsh1236I CGCG 1 cut(s) 670
Bsh1285I CGRYCG 3 cut(s) 482, 512, 674
BshFI GGCC 1 cut(s) 673
BshTI ACCGGT 1 cut(s) 605
BsiEI CGRYCG 3 cut(s) 482, 512, 674
BsiHKAI GWGCWC 2 cut(s) 32, 59
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 3 cut(s) 66, 170, 606
BsiWI CGTACG 1 cut(s) 215
BslI CCNNNNNNNGG 4 cut(s) 89, 514, 612, 631
BsnI GGCC 1 cut(s) 673
BsoBI CYCGRG 1 cut(s) 58
Bsp1286I GDGCHC 3 cut(s) 32, 59, 197
Bsp143I GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
Bsp1720I GCTNAGC 1 cut(s) 470
Bsp19I CCATGG 2 cut(s) 589, 700
BspACI CCGC 2 cut(s) 670, 674
BspANI GGCC 1 cut(s) 673
BspCNI CTCAG 3 cut(s) 44, 127, 708
BspFNI CGCG 1 cut(s) 670
BspLI GGNNCC 1 cut(s) 81
BspPI GGATC 3 cut(s) 137, 534, 667
BsrFI RCCGGY 1 cut(s) 605
BssAI RCCGGY 1 cut(s) 605
BssECI CCNNGG 4 cut(s) 578, 589, 700, 767
BssMI GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
BssT1I CCWWGG 3 cut(s) 578, 589, 700
Bst4CI ACNGT 2 cut(s) 322, 771
Bst6I CTCTTC 1 cut(s) 339
BstDEI CTNAG 5 cut(s) 31, 135, 405, 470, 695
BstDSI CCRYGG 3 cut(s) 589, 700, 767
BstF5I GGATG 5 cut(s) 160, 523, 528, 564, 599
BstFNI CGCG 1 cut(s) 670
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 7 cut(s) 132, 482, 512, 529, 662, 715, 722
BstMBI GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
BstMCI CGRYCG 3 cut(s) 482, 512, 674
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstSFI CTRYAG 1 cut(s) 188
BstUI CGCG 1 cut(s) 670
BstV1I GCAGC 3 cut(s) 232, 461, 478
BstZI CGGCCG 1 cut(s) 671
BsuRI GGCC 1 cut(s) 673
BtgI CCRYGG 3 cut(s) 589, 700, 767
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 5 cut(s) 160, 523, 528, 564, 599
CciNI GCGGCCGC 1 cut(s) 671
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 605
Csp6I GTAC 2 cut(s) 216, 402
CspAI ACCGGT 1 cut(s) 605
CviAII CATG 7 cut(s) 326, 336, 361, 379, 590, 681, 701
CviQI GTAC 2 cut(s) 216, 402
DdeI CTNAG 5 cut(s) 31, 135, 405, 470, 695
DpnI GATC 7 cut(s) 131, 481, 511, 528, 661, 714, 721
DpnII GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
DriI GACNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 1 cut(s) 671
EagI CGGCCG 1 cut(s) 671
Eam1104I CTCTTC 1 cut(s) 339
Eam1105I GACNNNNNGTC 1 cut(s) 511
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 2 cut(s) 30, 57
EclXI CGGCCG 1 cut(s) 671
Eco130I CCWWGG 3 cut(s) 578, 589, 700
Eco24I GRGCYC 3 cut(s) 32, 59, 197
Eco47III AGCGCT 1 cut(s) 180
Eco52I CGGCCG 1 cut(s) 671
Eco53kI GAGCTC 2 cut(s) 30, 57
Eco88I CYCGRG 1 cut(s) 58
EcoICRI GAGCTC 2 cut(s) 30, 57
EcoRI GAATTC 1 cut(s) 165
EcoT14I CCWWGG 3 cut(s) 578, 589, 700
EcoT38I GRGCYC 3 cut(s) 32, 59, 197
ErhI CCWWGG 3 cut(s) 578, 589, 700
FaeI CATG 7 cut(s) 329, 339, 364, 382, 593, 684, 704
FatI CATG 7 cut(s) 325, 335, 360, 378, 589, 680, 700
Fnu4HI GCNGC 5 cut(s) 246, 467, 475, 671, 674
FokI GGATG 5 cut(s) 167, 530, 535, 551, 606
FriOI GRGCYC 3 cut(s) 32, 59, 197
Fsp4HI GCNGC 5 cut(s) 246, 467, 475, 671, 674
GlaI GCGC 1 cut(s) 180
GluI GCNGC 5 cut(s) 246, 467, 475, 671, 674
HaeII RGCGCY 1 cut(s) 182
HaeIII GGCC 1 cut(s) 673
HapII CCGG 3 cut(s) 66, 170, 606
HhaI GCGC 1 cut(s) 181
Hin1II CATG 7 cut(s) 329, 339, 364, 382, 593, 684, 704
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HinfI GANTC 5 cut(s) 14, 74, 364, 646, 740
HpaII CCGG 3 cut(s) 66, 170, 606
HphI GGTGA 3 cut(s) 641, 716, 749
Hpy166II GTNNAC 2 cut(s) 41, 767
Hpy188I TCNGA 9 cut(s) 19, 79, 103, 164, 297, 487, 501, 517, 569
Hpy188III TCNNGA 2 cut(s) 33, 530
Hpy8I GTNNAC 2 cut(s) 41, 767
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 3 cut(s) 266, 406, 687
HpyCH4III ACNGT 2 cut(s) 322, 771
HpyCH4IV ACGT 2 cut(s) 422, 642
HpyCH4V TGCA 6 cut(s) 158, 203, 248, 325, 564, 597
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
HpyF3I CTNAG 5 cut(s) 31, 135, 405, 470, 695
HpySE526I ACGT 2 cut(s) 422, 642
Hsp92II CATG 7 cut(s) 329, 339, 364, 382, 593, 684, 704
HspAI GCGC 1 cut(s) 179
Kzo9I GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 7 cut(s) 18, 79, 176, 183, 189, 254, 619
Lsp1109I GCAGC 3 cut(s) 232, 461, 478
LweI GCATC 3 cut(s) 145, 573, 584
MaeII ACGT 2 cut(s) 422, 642
MaeIII GTNAC 1 cut(s) 737
MalI GATC 7 cut(s) 131, 481, 511, 528, 661, 714, 721
MboI GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
MboII GAAGA 3 cut(s) 268, 271, 356
MhlI GDGCHC 3 cut(s) 32, 59, 197
MluCI AATT 3 cut(s) 165, 350, 457
MlyI GAGTC 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 479, 495
MnlI CCTC 7 cut(s) 13, 111, 200, 235, 340, 537, 704
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 1 cut(s) 456
MspI CCGG 3 cut(s) 66, 170, 606
MvnI CGCG 1 cut(s) 670
MwoI GCNNNNNNNGC 1 cut(s) 314
NcoI CCATGG 2 cut(s) 589, 700
NdeII GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
NlaIII CATG 7 cut(s) 329, 339, 364, 382, 593, 684, 704
NlaIV GGNNCC 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 737
NotI GCGGCCGC 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 58
PcsI WCGNNNNNNNCGW 2 cut(s) 224, 505
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 4 cut(s) 14, 74, 364, 646
Pfl23II CGTACG 1 cut(s) 215
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 5 cut(s) 247, 468, 476, 672, 675
Ple19I CGATCG 2 cut(s) 482, 512
PleI GAGTC 1 cut(s) 734
PpsI GAGTC 1 cut(s) 734
Psp124BI GAGCTC 2 cut(s) 32, 59
PspLI CGTACG 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 81
PspXI VCTCGAGB 1 cut(s) 58
PvuI CGATCG 2 cut(s) 482, 512
RsaI GTAC 2 cut(s) 217, 403
RsaNI GTAC 2 cut(s) 216, 402
SacI GAGCTC 2 cut(s) 32, 59
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 5 cut(s) 246, 467, 475, 671, 674
Sau3AI GATC 7 cut(s) 129, 479, 509, 526, 659, 712, 719
SchI GAGTC 1 cut(s) 734
SduI GDGCHC 3 cut(s) 32, 59, 197
SfaNI GCATC 3 cut(s) 145, 573, 584
SfcI CTRYAG 1 cut(s) 188
Sfr274I CTCGAG 1 cut(s) 58
SlaI CTCGAG 1 cut(s) 58
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 3 cut(s) 165, 350, 457
SsiI CCGC 2 cut(s) 670, 674
SstI GAGCTC 2 cut(s) 32, 59
StyI CCWWGG 3 cut(s) 578, 589, 700
TaaI ACNGT 2 cut(s) 322, 771
TaiI ACGT 2 cut(s) 425, 645
TaqI TCGA 5 cut(s) 53, 59, 529, 542, 743
TaqII GACCGA 1 cut(s) 522
TasI AATT 3 cut(s) 165, 350, 457
TauI GCSGC 2 cut(s) 673, 676
TfiI GAWTC 4 cut(s) 14, 74, 364, 646
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TseFI GTSAC 1 cut(s) 737
TseI GCWGC 3 cut(s) 245, 466, 474
Tsp45I GTSAC 1 cut(s) 737
TspDTI ATGAA 3 cut(s) 269, 277, 669
TspGWI ACGGA 2 cut(s) 405, 504
XapI RAATTY 1 cut(s) 165
XhoI CTCGAG 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.