Rroxscaffold_4G00306100

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011149
Physical Location & Seq
Forward (+)
26914241 .. 26917581
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306100.1

Sequence Viewer

Length: 810 bp
ATGCCCAAACACTCTGATCCCTCAGACGAAGAAACCCCCGAGGACTCGAGCTCAGGCTCAGAGGAACAAAACCCACAAGATGGTGGTGTTCCAGAGTACGAGAAGCAGAGGCAAGCGAGAATTGCACACAACAGGGCCAGAATGGAGGCTCTGGGTCTGCCCAAAATCGCTTCCTCTCTAATGGGTTCGGCCCAGAATGCCACGAAGAGGAAGAGCAAGGGAAAAGCCAAGGTCTTGGAGGAGGACGATGATGATGAGTATATGCCTGAAGAGGCTTCTTCTAGTGAAGACGAAGCTAATGATGAGGATTTTGAGGATGAAAAATCATCTGGTGCGCGTAAGAAAAAGAATAAAGGTTCAAAACCTAAAAAGGCTCGTTCAAGAAGTAGTTTGAGCTACTCGGATTACCTTGATGATGATGAGGCATTGAAGCAGGCAATTGCTCTTTCATTACAAGATTCTGCTGTTTCAGGTGTGGTAAAAGCTAATGTTAATGAAAGAAAAGGAGAGAATCAAATTAAGCAAGATTCAGGGAGGAAGAGGAAAAACTCGCAGTTTGCTAGTCGGCTGCAGATGACAGAGGATGAGTTGGTTTTGCACTTCTTTCAGTTTGATGAGAAATGCAATGGGGGAATTTCTGCTAGAGATTTAGAAAGAGCAGCTGTTGCTCATGATTTTCTGTGGACTGATAAGGAGTTGGCTGACATGATTCATCTCTTTGATAGTGATGGGGATGCAAAGCTAAGTCTTGAAGATTTCCGGAAGATTGTTGTTCGCTGCAACATGATAAAAGCACCTGAAAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 EF-hand_8

Protein Analysis

269

Amino Acids

30.04

Weight (kDa)

5.03

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 197 - 256 4e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73440 AT4G00140
fragaria_vesca FvH4_7g11100 FvH4_7g11100
malus_domestica MD02G1210400.v1.1
prunus_persica Prupe.2G138200_v2.0.a1 Prupe.2G138200_v2.0.a1
pyrus_communis pycom02g17220 pycom07g10880
rosa_chinensis RchiOBHm_Chr1g0349121
rosa_laevigata RLG00000028604
rosa_multiflora Rmu_sc0008324.1_g000005 Rmu_sc0012100.1_g000007
rosa_roxburghii Rroxscaffold_4G00306100
rosa_rugosa Rorug01G0200300
rosa_samantha Rh1AG218000 Rh1BG183600 Rh1CG202400 Rh1DG213400
rosa_wichuraiana Rw1G018530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 80
AccII CGCG 1 cut(s) 337
AccIII TCCGGA 1 cut(s) 759
AclWI GGATC 1 cut(s) 11
AcsI RAATTY 2 cut(s) 633, 802
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 80, 207
AgsI TTSAA 4 cut(s) 360, 381, 430, 752
AluBI AGCT 6 cut(s) 51, 296, 396, 485, 662, 742
AluI AGCT 6 cut(s) 51, 296, 396, 485, 662, 742
Alw21I GWGCWC 1 cut(s) 53
AlwI GGATC 1 cut(s) 11
Ama87I CYCGRG 2 cut(s) 38, 46
Aor13HI TCCGGA 1 cut(s) 759
AoxI GGCC 2 cut(s) 135, 189
ApeKI GCWGC 3 cut(s) 568, 659, 777
ApoI RAATTY 2 cut(s) 633, 802
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 2 cut(s) 135, 190
AvaI CYCGRG 2 cut(s) 38, 46
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 555, 671, 764
BccI CCATC 2 cut(s) 74, 722
BfaI CTAG 3 cut(s) 282, 561, 642
BfmI CTRYAG 1 cut(s) 569
BisI GCNGC 3 cut(s) 569, 660, 778
BlsI GCNGC 3 cut(s) 570, 661, 779
BmeT110I CYCGRG 2 cut(s) 38, 46
BmgT120I GGNCC 2 cut(s) 135, 190
BmsI GCATC 1 cut(s) 724
BpiI GAAGAC 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 39, 228
BsaWI WCCGGW 1 cut(s) 759
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 207
Bse3DI GCAATG 1 cut(s) 631
BseAI TCCGGA 1 cut(s) 759
BseDI CCNNGG 2 cut(s) 39, 228
BseGI GGATG 3 cut(s) 322, 589, 739
BseLI CCNNNNNNNGG 2 cut(s) 80, 207
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 3 cut(s) 36, 66, 72
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 3 cut(s) 555, 671, 764
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 137, 191
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 38, 46
BsiSI CCGG 1 cut(s) 760
BslI CCNNNNNNNGG 2 cut(s) 80, 207
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 2 cut(s) 137, 191
BsoBI CYCGRG 2 cut(s) 38, 46
Bsp1286I GDGCHC 1 cut(s) 53
Bsp13I TCCGGA 1 cut(s) 759
Bsp143I GATC 1 cut(s) 16
BspANI GGCC 2 cut(s) 137, 191
BspCNI CTCAG 3 cut(s) 35, 65, 71
BspEI TCCGGA 1 cut(s) 759
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 2 cut(s) 670, 806
BspMAI CTGCAG 1 cut(s) 573
BspPI GGATC 1 cut(s) 11
BspQI GCTCTTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 631
BssECI CCNNGG 2 cut(s) 39, 228
BssMI GATC 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 228
Bst6I CTCTTC 4 cut(s) 200, 206, 264, 533
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 2 cut(s) 114, 435
BstDEI CTNAG 4 cut(s) 22, 52, 58, 743
BstF5I GGATG 3 cut(s) 322, 589, 739
BstFNI CGCG 1 cut(s) 337
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 3 cut(s) 122, 197, 665
BstSFI CTRYAG 1 cut(s) 569
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 3 cut(s) 555, 671, 764
BstV2I GAAGAC 1 cut(s) 294
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 2 cut(s) 137, 191
BtsCI GGATG 3 cut(s) 322, 589, 739
Cac8I GCNNGC 2 cut(s) 114, 435
CciI TCATGA 2 cut(s) 670, 806
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 135, 190
Csp6I GTAC 1 cut(s) 97
CviAII CATG 4 cut(s) 671, 706, 784, 807
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 4 cut(s) 22, 52, 58, 743
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
Eam1104I CTCTTC 4 cut(s) 200, 206, 264, 533
EarI CTCTTC 4 cut(s) 200, 206, 264, 533
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 38, 46
EcoICRI GAGCTC 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 4 cut(s) 674, 709, 787, 810
FaiI YATR 6 cut(s) 261, 263, 672, 707, 785, 808
FatI CATG 4 cut(s) 670, 705, 783, 806
Fnu4HI GCNGC 3 cut(s) 569, 660, 778
FokI GGATG 3 cut(s) 329, 596, 746
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 3 cut(s) 569, 660, 778
FspBI CTAG 3 cut(s) 282, 561, 642
GlaI GCGC 1 cut(s) 336
GluI GCNGC 3 cut(s) 569, 660, 778
HaeIII GGCC 2 cut(s) 137, 191
HapII CCGG 1 cut(s) 760
HhaI GCGC 1 cut(s) 337
Hin1II CATG 4 cut(s) 674, 709, 787, 810
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HinfI GANTC 5 cut(s) 44, 458, 511, 527, 709
HpaII CCGG 1 cut(s) 760
Hpy166II GTNNAC 1 cut(s) 684
Hpy188I TCNGA 4 cut(s) 16, 25, 61, 403
Hpy188III TCNNGA 6 cut(s) 92, 381, 671, 749, 760, 807
Hpy8I GTNNAC 1 cut(s) 684
HpyCH4V TGCA 6 cut(s) 125, 571, 598, 624, 737, 780
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 197, 665
HpyF3I CTNAG 4 cut(s) 22, 52, 58, 743
Hsp92II CATG 4 cut(s) 674, 709, 787, 810
HspAI GCGC 1 cut(s) 335
Kpn2I TCCGGA 1 cut(s) 759
Kzo9I GATC 1 cut(s) 16
LguI GCTCTTC 1 cut(s) 206
Lsp1109I GCAGC 3 cut(s) 555, 671, 764
LweI GCATC 1 cut(s) 724
MaeI CTAG 3 cut(s) 282, 561, 642
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 9 cut(s) 41, 217, 223, 270, 281, 299, 550, 764, 775
MfeI CAATTG 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 5 cut(s) 120, 438, 516, 633, 802
MlyI GAGTC 1 cut(s) 38
MroI TCCGGA 1 cut(s) 759
MseI TTAA 2 cut(s) 492, 519
MspA1I CMGCKG 1 cut(s) 662
MspI CCGG 1 cut(s) 760
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 202
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 3 cut(s) 122, 197, 665
NdeII GATC 1 cut(s) 16
NlaIII CATG 4 cut(s) 674, 709, 787, 810
PaeR7I CTCGAG 1 cut(s) 46
PagI TCATGA 2 cut(s) 670, 806
PciSI GCTCTTC 1 cut(s) 206
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 4 cut(s) 458, 511, 527, 709
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 3 cut(s) 570, 661, 779
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 53
PspPI GGNCC 2 cut(s) 135, 190
PspXI VCTCGAGB 1 cut(s) 46
PstI CTGCAG 1 cut(s) 573
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SacI GAGCTC 1 cut(s) 53
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 2 cut(s) 492, 519
SatI GCNGC 3 cut(s) 569, 660, 778
Sau3AI GATC 1 cut(s) 16
Sau96I GGNCC 2 cut(s) 135, 190
SchI GAGTC 1 cut(s) 38
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 1 cut(s) 724
SfcI CTRYAG 1 cut(s) 569
Sfr274I CTCGAG 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 5 cut(s) 120, 438, 516, 633, 802
SspMI CTAG 3 cut(s) 282, 561, 642
SstI GAGCTC 1 cut(s) 53
StyI CCWWGG 1 cut(s) 228
TaqI TCGA 1 cut(s) 47
TasI AATT 5 cut(s) 120, 438, 516, 633, 802
TfiI GAWTC 4 cut(s) 458, 511, 527, 709
Tru1I TTAA 2 cut(s) 492, 519
Tru9I TTAA 2 cut(s) 492, 519
TseI GCWGC 3 cut(s) 568, 659, 777
TspDTI ATGAA 5 cut(s) 333, 438, 510, 701, 795
Van91I CCANNNNNTGG 1 cut(s) 80
XapI RAATTY 2 cut(s) 633, 802
XhoI CTCGAG 1 cut(s) 46
XspI CTAG 3 cut(s) 282, 561, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.