Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009418

Physical Location & Seq

Genomic Coordinates Forward (+)

29268336 .. 29270700

Gene Structure 2365 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G77840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGGCGCTGCAGAACATTGGTGCTTCCAACCGTAACGATGCCTTCTACAGGTACAAGATGCCTAAGATGGTTACCAAAACCGAAGGCAAAGGTAATGGCATTAAGACCAACATTATCAACAATGTTGAGATTGCCAAAGCCTTGGCTAGACCGCCTTCTTATACGACCAAGTACTTTGGTTGTGAGCTTGGAGCGCAGTCTAAGTTTGATGAGAAGACTGGGACGTCGCTTGTGAATGGAGCTCACAACACGTCTAAGCTTGCTGGGCTTTTGGAGAATTTTATTAAGAAGTTTGTTCAGTGTTATGGATGTGGTAACCCGGAGACTGAGATTATTATTACGAAGACGCAGATGGTGAATCTCAAGTGTGCTGCTTGTGGGTTTATCTCTGAGGTCGACATGAGGGATAAGTTGACTAATTTCATTCTCAAGAACCCACCTGAGCAGAAGAAGGTGTCAAAGGATAAGAAAGCAATGAGGAAAGCTGAGAAGGAGAGGCTTAAAGAAGGCGAGCTAGCTGATGAGGAGCAGAGAAAGCTGAAAGCTAAGAAGAAAGCATTGTCTAACGGCAAGGATTCTAAGACGTCTAAGAACCATTCTTCTGATGAGGATATAAGCCCGAAGCATGATGAGAATGCTCTAGAGGTGGATGAGGATGAAGATGATGATGATGGTGTCGAGTGGCAAACTGATACTTCCCGAGAAGCTGCTGAGAAAAGAATGATGGAACAGTTGAGTGCTAAAACTGCCGAAATGGTGATGCTCTCTGCAATGGAAGTAGAAGAGAAAAAGGCGCCCAAAAGCAAATCTAACGGGAACGTTGTGAAAACTGAGAATCCTCCTCCGCAAGAGAAGAATCTCGTGCAGGATATGAAAGAGTATCTGAAGAAAGGGTCACCAATAAGCGCGCTCAAAAGTTTCATCTCGTCTCTCTCTGAACCTCCTCAAGACATCATGGACGCACTCTTCAATGCTCTCTTTGATGGTGTGGGAAAGGGATTCGCCAAAGAAGTGACTAAGAAGAAGAATTACTTAGCGGCTGCTGCAACAATGCAAGAGGATGGATCACAGATGCATCTGCTCAATTCGATTGGGACATTCTGTGGAAAGAATGGAAACGAAGAAGCTTTGAAAGAGGTGGCTCTGGTTCTTAAAGCATTGTACGACCAAGACATCATTGAGGAAGAGGTAGTGTTGGATTGGTACGAAAAGGGTCTCACCGGAGCTGACAAAAGCTCGCCGGTTTGGAAGAATGTTAAGCCTTTTGTGGAGTGGCTTCAGAGCGCTGAGTCTGAGTCCGAAGAGGAGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03013 ko04214 map03013 map04214

Pfam Domains

Protein Families

W2 eIF-5_eIF-2B

Protein Analysis

437

Amino Acids

48.64

Weight (kDa)

5.49

Isoelectric Point (pI)

37.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	11 - 127	2.9e-34	Domain found in IF2B/IF5
W2	PF02020	356 - 436	1.1e-22	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36730 AT1G77840
fragaria_vesca	FvH4_5g38780
malus_domestica	MD00G1104000.v1.1 MD09G1259500.v1.1 MD15G1359300.v1.1
prunus_persica	Prupe.1G506900_v2.0.a1 Prupe.3G151100_v2.0.a1
pyrus_communis	pycom08g14860 pycom09g17420 pycom15g32170
rosa_chinensis	RchiOBHm_Chr7g0240701
rosa_laevigata	RLG00000000625
rosa_multiflora	Rmu_sc0004799.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219650
rosa_rugosa	Rorug07G0338400 Rorug07G0338400
rosa_samantha	Rh7AG493000 Rh7BG465000 Rh7CG511400 Rh7DG478200
rosa_wichuraiana	Rw7G041700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	223
AatII	GACGTC	2 cut(s)	227, 587
AccB1I	GGYRCC	1 cut(s)	793
AccI	GTMKAC	1 cut(s)	396
AccII	CGCG	1 cut(s)	908
AciI	CCGC	3 cut(s)	152, 845, 1037
AclI	AACGTT	1 cut(s)	819
AclWI	GGATC	1 cut(s)	1072
AcsI	RAATTY	1 cut(s)	277
AcuI	CTGAAG	2 cut(s)	905, 1262
AcyI	GRCGYC	3 cut(s)	224, 584, 794
AfaI	GTAC	4 cut(s)	53, 173, 1163, 1205
AfeI	AGCGCT	1 cut(s)	1285
AfiI	CCNNNNNNNGG	1 cut(s)	48
AflIII	ACRYGT	1 cut(s)	249
AgsI	TTSAA	2 cut(s)	970, 1132
AjiI	CACGTC	1 cut(s)	252
Alw21I	GWGCWC	1 cut(s)	244
Alw26I	GTCTC	3 cut(s)	317, 933, 1220
AlwI	GGATC	1 cut(s)	1072
Ama87I	CYCGRG	1 cut(s)	699
Aor51HI	AGCGCT	1 cut(s)	1285
ApeKI	GCWGC	5 cut(s)	7, 371, 707, 1040, 1043
ApoI	RAATTY	1 cut(s)	277
AspLEI	GCGC	6 cut(s)	7, 196, 796, 908, 910, 1286
AsuC2I	CCSGG	1 cut(s)	320
AsuHPI	GGTGA	4 cut(s)	367, 769, 888, 1210
AsuNHI	GCTAGC	1 cut(s)	514
AvaI	CYCGRG	1 cut(s)	699
BanI	GGYRCC	1 cut(s)	793
BanII	GRGCYC	1 cut(s)	244
BarI	GAAGNNNNNNTAC	2 cut(s)	1013, 1045
BauI	CACGAG	1 cut(s)	860
BbsI	GAAGAC	2 cut(s)	221, 350
Bbv12I	GWGCWC	1 cut(s)	244
BbvI	GCAGC	4 cut(s)	358, 694, 1027, 1030
BccI	CCATC	6 cut(s)	61, 346, 665, 718, 977, 1055
BceAI	ACGGC	1 cut(s)	583
BcnI	CCSGG	1 cut(s)	320
BcoDI	GTCTC	3 cut(s)	317, 933, 1220
BfaI	CTAG	3 cut(s)	147, 515, 641
BfmI	CTRYAG	2 cut(s)	8, 46
BfoI	RGCGCY	3 cut(s)	8, 797, 1287
BisI	GCNGC	6 cut(s)	8, 372, 708, 1038, 1041, 1044
BlsI	GCNGC	6 cut(s)	9, 373, 709, 1039, 1042, 1045
BmcAI	AGTACT	1 cut(s)	173
Bme1390I	CCNGG	1 cut(s)	320
BmeT110I	CYCGRG	1 cut(s)	699
BmgBI	CACGTC	1 cut(s)	252
BmiI	GGNNCC	1 cut(s)	795
BmrFI	CCNGG	1 cut(s)	320
BmrI	ACTGGG	1 cut(s)	228
BmsI	GCATC	5 cut(s)	28, 48, 750, 1062, 1084
BmtI	GCTAGC	1 cut(s)	518
BmuI	ACTGGG	1 cut(s)	228
BpiI	GAAGAC	2 cut(s)	221, 350
Bpu10I	CCTNAGC	1 cut(s)	441
BpuEI	CTTGAG	3 cut(s)	347, 413, 930
BpuMI	CCSGG	1 cut(s)	320
BsaHI	GRCGYC	3 cut(s)	224, 584, 794
BsaI	GGTCTC	1 cut(s)	1220
BsaJI	CCNNGG	1 cut(s)	141
BsaWI	WCCGGW	1 cut(s)	1220
Bsc4I	CCNNNNNNNGG	1 cut(s)	48
Bse118I	RCCGGY	1 cut(s)	1240
Bse1I	ACTGG	1 cut(s)	223
Bse3DI	GCAATG	2 cut(s)	480, 777
BseDI	CCNNGG	1 cut(s)	141
BseGI	GGATG	4 cut(s)	314, 655, 661, 1066
BseLI	CCNNNNNNNGG	1 cut(s)	48
BseMI	GCAATG	2 cut(s)	480, 777
BseMII	CTCAG	8 cut(s)	318, 381, 432, 477, 702, 822, 1278, 1284
BseNI	ACTGG	1 cut(s)	223
BsePI	GCGCGC	1 cut(s)	906
BseRI	GAGGAG	3 cut(s)	539, 831, 933
BseXI	GCAGC	4 cut(s)	358, 694, 1027, 1030
BseYI	CCCAGC	1 cut(s)	263
BsgI	GTGCAG	1 cut(s)	884
Bsh1236I	CGCG	1 cut(s)	908
BshNI	GGYRCC	1 cut(s)	793
BsiHKAI	GWGCWC	1 cut(s)	244
BsiHKCI	CYCGRG	1 cut(s)	699
BsiSI	CCGG	3 cut(s)	320, 1221, 1241
BslFI	GGGAC	2 cut(s)	235, 1108
BslI	CCNNNNNNNGG	1 cut(s)	48
BsmAI	GTCTC	3 cut(s)	317, 933, 1220
BsmBI	CGTCTC	1 cut(s)	933
BsmFI	GGGAC	2 cut(s)	235, 1108
BsmI	GAATGC	1 cut(s)	640
Bso31I	GGTCTC	1 cut(s)	1220
BsoBI	CYCGRG	1 cut(s)	699
Bsp1286I	GDGCHC	1 cut(s)	244
Bsp143I	GATC	1 cut(s)	1064
BspACI	CCGC	3 cut(s)	152, 845, 1037
BspCNI	CTCAG	8 cut(s)	319, 382, 433, 478, 703, 823, 1279, 1285
BspFNI	CGCG	1 cut(s)	908
BspLI	GGNNCC	1 cut(s)	795
BspMAI	CTGCAG	1 cut(s)	12
BspOI	GCTAGC	1 cut(s)	518
BspPI	GGATC	1 cut(s)	1072
BspT107I	GGYRCC	1 cut(s)	793
BspTNI	GGTCTC	1 cut(s)	1220
BsrDI	GCAATG	2 cut(s)	480, 777
BsrFI	RCCGGY	1 cut(s)	1240
BsrI	ACTGG	1 cut(s)	223
BssAI	RCCGGY	1 cut(s)	1240
BssECI	CCNNGG	1 cut(s)	141
BssHII	GCGCGC	1 cut(s)	906
BssMI	GATC	1 cut(s)	1064
BssNI	GRCGYC	3 cut(s)	224, 584, 794
BssSI	CACGAG	1 cut(s)	860
BssT1I	CCWWGG	1 cut(s)	141
Bst2BI	CACGAG	1 cut(s)	860
Bst4CI	ACNGT	2 cut(s)	32, 732
Bst6I	CTCTTC	4 cut(s)	777, 971, 1179, 1296
BstACI	GRCGYC	3 cut(s)	224, 584, 794
BstC8I	GCNNGC	5 cut(s)	261, 512, 516, 908, 1238
BstEII	GGTNACC	3 cut(s)	70, 314, 894
BstENI	CCTNNNNNAGG	1 cut(s)	46
BstF5I	GGATG	4 cut(s)	314, 655, 661, 1066
BstFNI	CGCG	1 cut(s)	908
BstH2I	RGCGCY	3 cut(s)	8, 797, 1287
BstHHI	GCGC	6 cut(s)	7, 196, 796, 908, 910, 1286
BstKTI	GATC	1 cut(s)	1067
BstMAI	GTCTC	3 cut(s)	317, 933, 1220
BstMBI	GATC	1 cut(s)	1064
BstMWI	GCNNNNNNNGC	5 cut(s)	193, 265, 535, 746, 1043
BstPI	GGTNACC	3 cut(s)	70, 314, 894
BstSCI	CCNGG	1 cut(s)	318
BstSFI	CTRYAG	2 cut(s)	8, 46
BstUI	CGCG	1 cut(s)	908
BstV1I	GCAGC	4 cut(s)	358, 694, 1027, 1030
BstV2I	GAAGAC	2 cut(s)	221, 350
BstXI	CCANNNNNNTGG	1 cut(s)	142
BtrI	CACGTC	1 cut(s)	252
BtsCI	GGATG	4 cut(s)	314, 655, 661, 1066
BtsIMutI	CAGTG	1 cut(s)	305
Cac8I	GCNNGC	5 cut(s)	261, 512, 516, 908, 1238
CfoI	GCGC	6 cut(s)	7, 196, 796, 908, 910, 1286
Cfr10I	RCCGGY	1 cut(s)	1240
CseI	GACGC	2 cut(s)	355, 968
Csp6I	GTAC	4 cut(s)	52, 172, 1162, 1204
CviAII	CATG	3 cut(s)	400, 626, 955
CviQI	GTAC	4 cut(s)	52, 172, 1162, 1204
DinI	GGCGCC	1 cut(s)	795
DpnI	GATC	1 cut(s)	1066
DpnII	GATC	1 cut(s)	1064
DrdI	GACNNNNNNGTC	1 cut(s)	223
DseDI	GACNNNNNNGTC	1 cut(s)	223
Eam1104I	CTCTTC	4 cut(s)	777, 971, 1179, 1296
EarI	CTCTTC	4 cut(s)	777, 971, 1179, 1296
Ecl136II	GAGCTC	1 cut(s)	242
Eco130I	CCWWGG	1 cut(s)	141
Eco24I	GRGCYC	1 cut(s)	244
Eco31I	GGTCTC	1 cut(s)	1220
Eco47III	AGCGCT	1 cut(s)	1285
Eco53kI	GAGCTC	1 cut(s)	242
Eco57I	CTGAAG	2 cut(s)	905, 1262
Eco88I	CYCGRG	1 cut(s)	699
Eco91I	GGTNACC	3 cut(s)	70, 314, 894
EcoICRI	GAGCTC	1 cut(s)	242
EcoNI	CCTNNNNNAGG	1 cut(s)	46
EcoO65I	GGTNACC	3 cut(s)	70, 314, 894
EcoT14I	CCWWGG	1 cut(s)	141
EcoT22I	ATGCAT	1 cut(s)	1077
EcoT38I	GRGCYC	1 cut(s)	244
EgeI	GGCGCC	1 cut(s)	795
EheI	GGCGCC	1 cut(s)	795
ErhI	CCWWGG	1 cut(s)	141
Esp3I	CGTCTC	1 cut(s)	933
FaeI	CATG	3 cut(s)	403, 629, 958
FaiI	YATR	7 cut(s)	162, 306, 401, 614, 627, 872, 956
FalI	AAGNNNNNCTT	2 cut(s)	1016, 1048
FaqI	GGGAC	2 cut(s)	235, 1108
FatI	CATG	3 cut(s)	399, 625, 954
FblI	GTMKAC	1 cut(s)	396
Fnu4HI	GCNGC	6 cut(s)	8, 372, 708, 1038, 1041, 1044
FokI	GGATG	4 cut(s)	321, 662, 668, 1073
FriOI	GRGCYC	1 cut(s)	244
Fsp4HI	GCNGC	6 cut(s)	8, 372, 708, 1038, 1041, 1044
FspBI	CTAG	3 cut(s)	147, 515, 641
GlaI	GCGC	6 cut(s)	6, 195, 795, 907, 909, 1285
GluI	GCNGC	6 cut(s)	8, 372, 708, 1038, 1041, 1044
GsaI	CCCAGC	1 cut(s)	267
HaeII	RGCGCY	3 cut(s)	8, 797, 1287
HapII	CCGG	3 cut(s)	320, 1221, 1241
HgaI	GACGC	2 cut(s)	355, 968
HhaI	GCGC	6 cut(s)	7, 196, 796, 908, 910, 1286
Hin1I	GRCGYC	3 cut(s)	224, 584, 794
Hin1II	CATG	3 cut(s)	403, 629, 958
Hin6I	GCGC	6 cut(s)	5, 194, 794, 906, 908, 1284
HinP1I	GCGC	6 cut(s)	5, 194, 794, 906, 908, 1284
HincII	GTYRAC	2 cut(s)	397, 414
HindII	GTYRAC	2 cut(s)	397, 414
HindIII	AAGCTT	2 cut(s)	257, 1125
HinfI	GANTC	7 cut(s)	358, 575, 835, 856, 999, 1289, 1295
HpaII	CCGG	3 cut(s)	320, 1221, 1241
HphI	GGTGA	4 cut(s)	367, 769, 888, 1210
Hpy166II	GTNNAC	2 cut(s)	397, 414
Hpy188I	TCNGA	7 cut(s)	391, 604, 885, 937, 1281, 1294, 1300
Hpy188III	TCNNGA	4 cut(s)	430, 641, 699, 947
Hpy8I	GTNNAC	2 cut(s)	397, 414
Hpy99I	CGWCG	1 cut(s)	229
HpyAV	CCTTC	6 cut(s)	52, 77, 165, 445, 484, 500
HpyCH4III	ACNGT	2 cut(s)	32, 732
HpyCH4IV	ACGT	4 cut(s)	224, 251, 584, 819
HpyCH4V	TGCA	6 cut(s)	10, 770, 865, 1046, 1054, 1075
HpyF10VI	GCNNNNNNNGC	5 cut(s)	193, 265, 535, 746, 1043
HpySE526I	ACGT	4 cut(s)	224, 251, 584, 819
Hsp92I	GRCGYC	3 cut(s)	224, 584, 794
Hsp92II	CATG	3 cut(s)	403, 629, 958
HspAI	GCGC	6 cut(s)	5, 194, 794, 906, 908, 1284
KasI	GGCGCC	1 cut(s)	793
Kzo9I	GATC	1 cut(s)	1064
LmnI	GCTCC	4 cut(s)	191, 239, 526, 1223
LpnPI	CCDG	9 cut(s)	34, 204, 249, 333, 453, 851, 1130, 1234, 1254
Lsp1109I	GCAGC	4 cut(s)	358, 694, 1027, 1030
LweI	GCATC	5 cut(s)	28, 48, 750, 1062, 1084
MaeI	CTAG	3 cut(s)	147, 515, 641
MaeII	ACGT	4 cut(s)	224, 251, 584, 819
MaeIII	GTNAC	5 cut(s)	32, 70, 314, 894, 1012
MalI	GATC	1 cut(s)	1066
MboI	GATC	1 cut(s)	1064
MhlI	GDGCHC	1 cut(s)	244
MluCI	AATT	4 cut(s)	277, 418, 1027, 1084
Mly113I	GGCGCC	1 cut(s)	794
MlyI	GAGTC	2 cut(s)	1298, 1304
MmeI	TCCRAC	2 cut(s)	51, 1176
Mph1103I	ATGCAT	1 cut(s)	1077
MseI	TTAA	5 cut(s)	102, 285, 501, 1152, 1257
MspI	CCGG	3 cut(s)	320, 1221, 1241
MspR9I	CCNGG	1 cut(s)	320
Mva1269I	GAATGC	1 cut(s)	640
MvnI	CGCG	1 cut(s)	908
MwoI	GCNNNNNNNGC	5 cut(s)	193, 265, 535, 746, 1043
NarI	GGCGCC	1 cut(s)	794
NciI	CCSGG	1 cut(s)	320
NdeII	GATC	1 cut(s)	1064
NheI	GCTAGC	1 cut(s)	514
NlaIII	CATG	3 cut(s)	403, 629, 958
NlaIV	GGNNCC	1 cut(s)	795
NmuCI	GTSAC	2 cut(s)	894, 1012
NsiI	ATGCAT	1 cut(s)	1077
PauI	GCGCGC	1 cut(s)	906
PctI	GAATGC	1 cut(s)	640
PfeI	GAWTC	5 cut(s)	358, 575, 835, 856, 999
PkrI	GCNGC	6 cut(s)	9, 373, 709, 1039, 1042, 1045
PleI	GAGTC	2 cut(s)	1297, 1303
PluTI	GGCGCC	1 cut(s)	797
PpsI	GAGTC	2 cut(s)	1297, 1303
Psp124BI	GAGCTC	1 cut(s)	244
Psp1406I	AACGTT	1 cut(s)	819
PspEI	GGTNACC	3 cut(s)	70, 314, 894
PspFI	CCCAGC	1 cut(s)	263
PspN4I	GGNNCC	1 cut(s)	795
PstI	CTGCAG	1 cut(s)	12
PteI	GCGCGC	1 cut(s)	906
RsaI	GTAC	4 cut(s)	53, 173, 1163, 1205
RsaNI	GTAC	4 cut(s)	52, 172, 1162, 1204
SacI	GAGCTC	1 cut(s)	244
SalI	GTCGAC	1 cut(s)	395
SaqAI	TTAA	5 cut(s)	102, 285, 501, 1152, 1257
SatI	GCNGC	6 cut(s)	8, 372, 708, 1038, 1041, 1044
Sau3AI	GATC	1 cut(s)	1064
ScaI	AGTACT	1 cut(s)	173
SchI	GAGTC	2 cut(s)	1298, 1304
ScrFI	CCNGG	1 cut(s)	320
SduI	GDGCHC	1 cut(s)	244
SfaNI	GCATC	5 cut(s)	28, 48, 750, 1062, 1084
SfcI	CTRYAG	2 cut(s)	8, 46
SfoI	GGCGCC	1 cut(s)	795
SmlI	CTYRAG	3 cut(s)	362, 428, 945
SmoI	CTYRAG	3 cut(s)	362, 428, 945
Sse9I	AATT	4 cut(s)	277, 418, 1027, 1084
SsiI	CCGC	3 cut(s)	152, 845, 1037
SspDI	GGCGCC	1 cut(s)	793
SspMI	CTAG	3 cut(s)	147, 515, 641
SstI	GAGCTC	1 cut(s)	244
StyD4I	CCNGG	1 cut(s)	318
StyI	CCWWGG	1 cut(s)	141
TaaI	ACNGT	2 cut(s)	32, 732
TaiI	ACGT	4 cut(s)	227, 254, 587, 822
TaqI	TCGA	3 cut(s)	396, 678, 1088
TasI	AATT	4 cut(s)	277, 418, 1027, 1084
TatI	WGTACW	1 cut(s)	171
TauI	GCSGC	1 cut(s)	1040
TfiI	GAWTC	5 cut(s)	358, 575, 835, 856, 999
Tru1I	TTAA	5 cut(s)	102, 285, 501, 1152, 1257
Tru9I	TTAA	5 cut(s)	102, 285, 501, 1152, 1257
TscAI	CASTG	1 cut(s)	305
TseFI	GTSAC	2 cut(s)	894, 1012
TseI	GCWGC	5 cut(s)	7, 371, 707, 1040, 1043
Tsp45I	GTSAC	2 cut(s)	894, 1012
TspDTI	ATGAA	4 cut(s)	412, 672, 887, 910
TspRI	CASTG	1 cut(s)	305
XagI	CCTNNNNNAGG	1 cut(s)	46
XapI	RAATTY	1 cut(s)	277
XbaI	TCTAGA	1 cut(s)	640
XmiI	GTMKAC	1 cut(s)	396
XspI	CTAG	3 cut(s)	147, 515, 641
ZraI	GACGTC	2 cut(s)	225, 585
ZrmI	AGTACT	1 cut(s)	173
Zsp2I	ATGCAT	1 cut(s)	1077

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.