Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009418

Physical Location & Seq

Genomic Coordinates Forward (+)

43338265 .. 43341429

Gene Structure 3165 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1359300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGCTCTACAAAACATCGGTGCTTCAAACCGTGATGATGCCTTCTACAGGTATAAGATGCCAAGGATGATCACAAAGATTGAAGGCAGAGGAAATGGCATCAAGACGAATGTGGTTAATATGGTCGATATTGCAAAGGCCTTGGCTAGACCTGCTTCGTACACCACGAAGTATTTTGGCTGTGAGCTTGGAGCCCAATCAAAATTTGATGAGAAGACTGGAACTTCCCATGTGAATGGTGCTCATGACACTGCAAAGCTTGCTGGACTTCTTGAGAACTTCATTAAGAAATATGTGCAGTGCTATGGTTGCGGGAATCCAGAAACTGAAATTATTATAACCAAGACACAGATGCTAAATCTGAAATGTGCTGCATGTGGGTTTGTATCTGATGTTGATATGAGGGACAAGCTTACGACTTTTATCCTCAAGAATCCTCCAGAGCAGAAGAAGACAGCTAAAGAAAAGAAAGCCTTGAGGAGGGCTGACAAAGAACAACTTAAGGAAGGGGAGGCCGCTGACGAGGAGCAAAAGAAGCTTAAGAAGGAGGCAGCAAAGAAGAAAGGAACTTCTGGTTCAAAAGCATCAAGCAAGAAGAAAAACACTGGTTCCGATGAGGATCGCTCCCCAACTCACAGCCAGGGAGATGAGAATGACCAAGCTGCTGAGGAGGACGATGATGACGATGTCCAGTGGCAGACGGACACTTCCTTGGAGGCAGCTAAGAAGCGAATCCAGGAGCAGTTGAGTGCTGTTACTGCTGATATGGTGATGCTTTCTACCGTTGAAGAGGAGAAAAGGTCACCAAAGAAGTCTCCCGATCATGAAGCGAAGAAGCCTGAGACGAAGAGTCATCAGAACGGTGCTGATAATAATACCCATGAAAGACTTGTTAATGAGATCAAACAATCCTTGCAAAAGGGGACTTCTGCAGCTCGGCTTAAATCCTATTTGGGGACTCTTTCTGGCACACCCCAAGAAGTTATGGACGCCCTACTCGAGGCTCTCTTTGAAGGTGTTGGAAAGGGGTTCTCGAAAGTGGTTTCCAAGAAGAAAAACTACCTCGCTGCAGCCACCCAGACAGAGGGATCTCAGACGGTTCTATTGCAAGCTATCGAGTCCTTCAGTGGGAAGGCAAACCCTGATGCCGCAAAGGAAGTGGCTCTGGTTCTGAAATCATTGTATGATGTTGACATTCTGGAGGAGGAATACATTATGAAGTGGTACCAACAGGGCTTAGCTGGGAACAACAAGAGCTCCCCTATTTGGAAGAACTCCAAGCCGTTTATCGAATGGCTCCAGAGCGCCGAGTCGGAGTCAGAGCAAGAGCCTTCTGTTCACCCTTCAGCTGCGCTGTTGATGTCTTTGGTTTTAATGTCGAGTGCGAGTCATTTGGTGGTGGTGGTGGTGGTGGTCTGTGTGATTGTAAGTGACATTGGTTTTCTTTTGCATTTGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03013 ko04214 map03013 map04214

Pfam Domains

Protein Families

W2 eIF-5_eIF-2B

Protein Analysis

487

Amino Acids

53.41

Weight (kDa)

6.79

Isoelectric Point (pI)

39.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	11 - 127	2.2e-34	Domain found in IF2B/IF5
W2	PF02020	365 - 443	1.4e-19	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36730 AT1G77840
fragaria_vesca	FvH4_5g38780
malus_domestica	MD00G1104000.v1.1 MD09G1259500.v1.1 MD15G1359300.v1.1
prunus_persica	Prupe.1G506900_v2.0.a1 Prupe.3G151100_v2.0.a1
pyrus_communis	pycom08g14860 pycom09g17420 pycom15g32170
rosa_chinensis	RchiOBHm_Chr7g0240701
rosa_laevigata	RLG00000000625
rosa_multiflora	Rmu_sc0004799.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219650
rosa_rugosa	Rorug07G0338400 Rorug07G0338400
rosa_samantha	Rh7AG493000 Rh7BG465000 Rh7CG511400 Rh7DG478200
rosa_wichuraiana	Rw7G041700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	338
Acc36I	ACCTGC	1 cut(s)	160
Acc65I	GGTACC	1 cut(s)	1224
AccB1I	GGYRCC	1 cut(s)	1224
AciI	CCGC	3 cut(s)	312, 516, 1149
AclWI	GGATC	2 cut(s)	627, 1096
AcsI	RAATTY	1 cut(s)	203
AcuI	CTGAAG	2 cut(s)	1108, 1329
AcyI	GRCGYC	1 cut(s)	990
AfaI	GTAC	2 cut(s)	161, 1226
AfiI	CCNNNNNNNGG	7 cut(s)	48, 480, 954, 1000, 1084, 1128, 1266
AflII	CTTAAG	2 cut(s)	500, 539
AgsI	TTSAA	5 cut(s)	27, 83, 579, 788, 1013
AhdI	GACNNNNNGTC	1 cut(s)	849
AjnI	CCWGG	2 cut(s)	639, 735
Alw21I	GWGCWC	2 cut(s)	244, 1259
Alw26I	GTCTC	2 cut(s)	819, 836
AlwI	GGATC	2 cut(s)	627, 1096
AlwNI	CAGNNNCTG	1 cut(s)	326
Ama87I	CYCGRG	1 cut(s)	998
AoxI	GGCC	2 cut(s)	138, 513
ApeKI	GCWGC	8 cut(s)	371, 551, 662, 719, 932, 1067, 1070, 1349
ApoI	RAATTY	1 cut(s)	203
Asp718I	GGTACC	1 cut(s)	1224
AspLEI	GCGC	2 cut(s)	1307, 1354
AsuHPI	GGTGA	3 cut(s)	781, 795, 1331
AvaI	CYCGRG	1 cut(s)	998
BanI	GGYRCC	1 cut(s)	1224
BanII	GRGCYC	2 cut(s)	196, 1259
BarI	GAAGNNNNNNTAC	2 cut(s)	1043, 1075
BbsI	GAAGAC	2 cut(s)	221, 458
Bbv12I	GWGCWC	2 cut(s)	244, 1259
BbvCI	CCTCAGC	1 cut(s)	666
BbvI	GCAGC	8 cut(s)	358, 563, 649, 731, 944, 1054, 1082, 1336
BceAI	ACGGC	1 cut(s)	1267
BciT130I	CCWGG	2 cut(s)	641, 737
BclI	TGATCA	1 cut(s)	69
BcoDI	GTCTC	2 cut(s)	819, 836
BfaI	CTAG	1 cut(s)	147
BfmI	CTRYAG	3 cut(s)	46, 930, 1068
BfoI	RGCGCY	1 cut(s)	1308
BfrI	CTTAAG	2 cut(s)	500, 539
BfuAI	ACCTGC	1 cut(s)	160
BlpI	GCTNAGC	1 cut(s)	1237
Bme1390I	CCNGG	2 cut(s)	641, 737
BmeRI	GACNNNNNGTC	1 cut(s)	849
BmeT110I	CYCGRG	1 cut(s)	998
BmiI	GGNNCC	4 cut(s)	193, 610, 1226, 1298
BmrFI	CCNGG	2 cut(s)	641, 737
BmsI	GCATC	7 cut(s)	28, 48, 108, 342, 593, 762, 1135
BpiI	GAAGAC	2 cut(s)	221, 458
BplI	GAGNNNNNCTC	2 cut(s)	608, 640
BpmI	CTGGAG	3 cut(s)	423, 1220, 1283
Bpu10I	CCTNAGC	1 cut(s)	666
Bpu1102I	GCTNAGC	1 cut(s)	1237
BpuEI	CTTGAG	3 cut(s)	293, 413, 496
BsaBI	GATNNNNATC	1 cut(s)	618
BsaHI	GRCGYC	1 cut(s)	990
BsaJI	CCNNGG	4 cut(s)	62, 141, 640, 711
BsaXI	ACNNNNNCTCC	4 cut(s)	785, 815, 1241, 1271
Bsc4I	CCNNNNNNNGG	7 cut(s)	48, 480, 954, 1000, 1084, 1128, 1266
Bse1I	ACTGG	3 cut(s)	223, 610, 691
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	2 cut(s)	641, 737
BseDI	CCNNGG	4 cut(s)	62, 141, 640, 711
BseGI	GGATG	1 cut(s)	72
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	7 cut(s)	48, 480, 954, 1000, 1084, 1128, 1266
BseMII	CTCAG	3 cut(s)	657, 831, 1106
BseNI	ACTGG	3 cut(s)	223, 610, 691
BseRI	GAGGAG	5 cut(s)	493, 539, 683, 806, 1217
BseXI	GCAGC	8 cut(s)	358, 563, 649, 731, 944, 1054, 1082, 1336
BseYI	CCCAGC	1 cut(s)	1241
BsgI	GTGCAG	1 cut(s)	317
BshFI	GGCC	2 cut(s)	140, 515
BshNI	GGYRCC	1 cut(s)	1224
BsiHKAI	GWGCWC	2 cut(s)	244, 1259
BsiHKCI	CYCGRG	1 cut(s)	998
BslFI	GGGAC	3 cut(s)	419, 937, 970
BslI	CCNNNNNNNGG	7 cut(s)	48, 480, 954, 1000, 1084, 1128, 1266
BsmAI	GTCTC	2 cut(s)	819, 836
BsmBI	CGTCTC	1 cut(s)	836
BsmFI	GGGAC	3 cut(s)	419, 937, 970
BsnI	GGCC	2 cut(s)	140, 515
BsoBI	CYCGRG	1 cut(s)	998
Bsp1286I	GDGCHC	3 cut(s)	196, 244, 1259
Bsp143I	GATC	5 cut(s)	69, 619, 820, 900, 1088
Bsp1720I	GCTNAGC	1 cut(s)	1237
BspACI	CCGC	3 cut(s)	312, 516, 1149
BspANI	GGCC	2 cut(s)	140, 515
BspCNI	CTCAG	3 cut(s)	658, 832, 1105
BspHI	TCATGA	2 cut(s)	244, 823
BspLI	GGNNCC	4 cut(s)	193, 610, 1226, 1298
BspMAI	CTGCAG	2 cut(s)	934, 1072
BspMI	ACCTGC	1 cut(s)	160
BspPI	GGATC	2 cut(s)	627, 1096
BspT107I	GGYRCC	1 cut(s)	1224
BspTI	CTTAAG	2 cut(s)	500, 539
BsrI	ACTGG	3 cut(s)	223, 610, 691
BssECI	CCNNGG	4 cut(s)	62, 141, 640, 711
BssMI	GATC	5 cut(s)	69, 619, 820, 900, 1088
BssNI	GRCGYC	1 cut(s)	990
BssT1I	CCWWGG	3 cut(s)	62, 141, 711
Bst2UI	CCWGG	2 cut(s)	641, 737
Bst4CI	ACNGT	4 cut(s)	32, 784, 863, 1099
Bst6I	CTCTTC	2 cut(s)	783, 842
BstACI	GRCGYC	1 cut(s)	990
BstAFI	CTTAAG	2 cut(s)	500, 539
BstAPI	GCANNNNNTGC	1 cut(s)	260
BstC8I	GCNNGC	2 cut(s)	261, 1110
BstDEI	CTNAG	5 cut(s)	666, 723, 840, 1092, 1237
BstEII	GGTNACC	1 cut(s)	801
BstENI	CCTNNNNNAGG	3 cut(s)	46, 478, 998
BstF5I	GGATG	1 cut(s)	72
BstH2I	RGCGCY	1 cut(s)	1308
BstHHI	GCGC	2 cut(s)	1307, 1354
BstKTI	GATC	5 cut(s)	72, 622, 823, 903, 1091
BstMAI	GTCTC	2 cut(s)	819, 836
BstMBI	GATC	5 cut(s)	69, 619, 820, 900, 1088
BstMWI	GCNNNNNNNGC	5 cut(s)	152, 260, 309, 535, 758
BstNI	CCWGG	2 cut(s)	641, 737
BstNSI	RCATGY	1 cut(s)	378
BstPI	GGTNACC	1 cut(s)	801
BstSCI	CCNGG	2 cut(s)	639, 735
BstSFI	CTRYAG	3 cut(s)	46, 930, 1068
BstV1I	GCAGC	8 cut(s)	358, 563, 649, 731, 944, 1054, 1082, 1336
BstV2I	GAAGAC	2 cut(s)	221, 458
BstX2I	RGATCY	1 cut(s)	1088
BstXI	CCANNNNNNTGG	1 cut(s)	236
BstYI	RGATCY	1 cut(s)	1088
BsuRI	GGCC	2 cut(s)	140, 515
BtsCI	GGATG	1 cut(s)	72
BtsI	GCAGTG	2 cut(s)	249, 305
BtsIMutI	CAGTG	5 cut(s)	249, 305, 603, 698, 1132
BveI	ACCTGC	1 cut(s)	160
Cac8I	GCNNGC	2 cut(s)	261, 1110
CaiI	CAGNNNCTG	1 cut(s)	326
CciI	TCATGA	2 cut(s)	244, 823
CfoI	GCGC	2 cut(s)	1307, 1354
CseI	GACGC	1 cut(s)	998
Csp6I	GTAC	2 cut(s)	160, 1225
CspCI	CAANNNNNGTGG	2 cut(s)	1140, 1175
CviAII	CATG	5 cut(s)	230, 245, 375, 824, 881
CviQI	GTAC	2 cut(s)	160, 1225
DdeI	CTNAG	5 cut(s)	666, 723, 840, 1092, 1237
DpnI	GATC	5 cut(s)	71, 621, 822, 902, 1090
DpnII	GATC	5 cut(s)	69, 619, 820, 900, 1088
DriI	GACNNNNNGTC	1 cut(s)	849
Eam1104I	CTCTTC	2 cut(s)	783, 842
Eam1105I	GACNNNNNGTC	1 cut(s)	849
EarI	CTCTTC	2 cut(s)	783, 842
Ecl136II	GAGCTC	1 cut(s)	1257
Eco130I	CCWWGG	3 cut(s)	62, 141, 711
Eco147I	AGGCCT	1 cut(s)	140
Eco24I	GRGCYC	2 cut(s)	196, 1259
Eco53kI	GAGCTC	1 cut(s)	1257
Eco57I	CTGAAG	2 cut(s)	1108, 1329
Eco88I	CYCGRG	1 cut(s)	998
Eco91I	GGTNACC	1 cut(s)	801
EcoICRI	GAGCTC	1 cut(s)	1257
EcoNI	CCTNNNNNAGG	3 cut(s)	46, 478, 998
EcoO65I	GGTNACC	1 cut(s)	801
EcoRII	CCWGG	2 cut(s)	639, 735
EcoT14I	CCWWGG	3 cut(s)	62, 141, 711
EcoT38I	GRGCYC	2 cut(s)	196, 1259
ErhI	CCWWGG	3 cut(s)	62, 141, 711
Esp3I	CGTCTC	1 cut(s)	836
FaeI	CATG	5 cut(s)	233, 248, 378, 827, 884
FalI	AAGNNNNNCTT	4 cut(s)	458, 490, 483, 515
FaqI	GGGAC	3 cut(s)	419, 937, 970
FatI	CATG	5 cut(s)	229, 244, 374, 823, 880
FauI	CCCGC	1 cut(s)	305
FbaI	TGATCA	1 cut(s)	69
FokI	GGATG	1 cut(s)	79
FriOI	GRGCYC	2 cut(s)	196, 1259
FspBI	CTAG	1 cut(s)	147
GlaI	GCGC	2 cut(s)	1306, 1353
GsaI	CCCAGC	1 cut(s)	1245
GsuI	CTGGAG	3 cut(s)	423, 1220, 1283
HaeII	RGCGCY	1 cut(s)	1308
HaeIII	GGCC	2 cut(s)	140, 515
HgaI	GACGC	1 cut(s)	998
HhaI	GCGC	2 cut(s)	1307, 1354
Hin1I	GRCGYC	1 cut(s)	990
Hin1II	CATG	5 cut(s)	233, 248, 378, 827, 884
Hin6I	GCGC	2 cut(s)	1305, 1352
HinP1I	GCGC	2 cut(s)	1305, 1352
HincII	GTYRAC	1 cut(s)	1192
HindII	GTYRAC	1 cut(s)	1192
HindIII	AAGCTT	3 cut(s)	257, 410, 536
HinfI	GANTC	9 cut(s)	316, 433, 732, 850, 958, 1118, 1310, 1316, 1387
HphI	GGTGA	3 cut(s)	781, 795, 1331
Hpy166II	GTNNAC	3 cut(s)	162, 1192, 1339
Hpy188I	TCNGA	8 cut(s)	363, 391, 613, 858, 1095, 1173, 1315, 1321
Hpy8I	GTNNAC	3 cut(s)	162, 1192, 1339
HpyAV	CCTTC	9 cut(s)	52, 77, 500, 538, 1007, 1126, 1132, 1341, 1353
HpyCH4III	ACNGT	4 cut(s)	32, 784, 863, 1099
HpyCH4V	TGCA	9 cut(s)	134, 254, 298, 374, 916, 932, 1070, 1108, 1450
HpyF10VI	GCNNNNNNNGC	5 cut(s)	152, 260, 309, 535, 758
HpyF3I	CTNAG	5 cut(s)	666, 723, 840, 1092, 1237
Hsp92I	GRCGYC	1 cut(s)	990
Hsp92II	CATG	5 cut(s)	233, 248, 378, 827, 884
HspAI	GCGC	2 cut(s)	1305, 1352
KpnI	GGTACC	1 cut(s)	1228
Ksp22I	TGATCA	1 cut(s)	69
Kzo9I	GATC	5 cut(s)	69, 619, 820, 900, 1088
LmnI	GCTCC	6 cut(s)	191, 526, 629, 739, 1262, 1302
Lsp1109I	GCAGC	8 cut(s)	358, 563, 649, 731, 944, 1054, 1082, 1336
LweI	GCATC	7 cut(s)	28, 48, 108, 342, 593, 762, 1135
MaeI	CTAG	1 cut(s)	147
MaeIII	GTNAC	3 cut(s)	754, 801, 1430
MalI	GATC	5 cut(s)	71, 621, 822, 902, 1090
MboI	GATC	5 cut(s)	69, 619, 820, 900, 1088
MflI	RGATCY	1 cut(s)	1088
MhlI	GDGCHC	3 cut(s)	196, 244, 1259
MluCI	AATT	2 cut(s)	203, 330
MlyI	GAGTC	6 cut(s)	859, 952, 1127, 1319, 1325, 1396
MmeI	TCCRAC	2 cut(s)	1000, 1293
MseI	TTAA	8 cut(s)	117, 285, 501, 540, 894, 942, 1373, 1459
MslI	CAYNNNNRTG	1 cut(s)	234
MspA1I	CMGCKG	2 cut(s)	518, 1349
MspCI	CTTAAG	2 cut(s)	500, 539
MspR9I	CCNGG	2 cut(s)	641, 737
MvaI	CCWGG	2 cut(s)	641, 737
MwoI	GCNNNNNNNGC	5 cut(s)	152, 260, 309, 535, 758
NdeII	GATC	5 cut(s)	69, 619, 820, 900, 1088
NlaIII	CATG	5 cut(s)	233, 248, 378, 827, 884
NlaIV	GGNNCC	4 cut(s)	193, 610, 1226, 1298
NmeAIII	GCCGAG	2 cut(s)	916, 1333
NmuCI	GTSAC	2 cut(s)	801, 1430
NspI	RCATGY	1 cut(s)	378
PaeR7I	CTCGAG	1 cut(s)	998
PagI	TCATGA	2 cut(s)	244, 823
PceI	AGGCCT	1 cut(s)	140
PcsI	WCGNNNNNNNCGW	3 cut(s)	164, 681, 996
PfeI	GAWTC	3 cut(s)	316, 433, 732
PflFI	GACNNNGTC	1 cut(s)	686
PfoI	TCCNGGA	1 cut(s)	735
PleI	GAGTC	6 cut(s)	858, 952, 1126, 1318, 1324, 1395
PpsI	GAGTC	6 cut(s)	858, 952, 1126, 1318, 1324, 1395
PsiI	TTATAA	1 cut(s)	338
Psp124BI	GAGCTC	1 cut(s)	1259
Psp6I	CCWGG	2 cut(s)	639, 735
PspEI	GGTNACC	1 cut(s)	801
PspFI	CCCAGC	1 cut(s)	1241
PspGI	CCWGG	2 cut(s)	639, 735
PspN4I	GGNNCC	4 cut(s)	193, 610, 1226, 1298
PspXI	VCTCGAGB	1 cut(s)	998
PstI	CTGCAG	2 cut(s)	934, 1072
PstNI	CAGNNNCTG	1 cut(s)	326
PsuI	RGATCY	1 cut(s)	1088
PsyI	GACNNNGTC	1 cut(s)	686
PvuII	CAGCTG	1 cut(s)	1349
RsaI	GTAC	2 cut(s)	161, 1226
RsaNI	GTAC	2 cut(s)	160, 1225
RseI	CAYNNNNRTG	1 cut(s)	234
SacI	GAGCTC	1 cut(s)	1259
SaqAI	TTAA	8 cut(s)	117, 285, 501, 540, 894, 942, 1373, 1459
Sau3AI	GATC	5 cut(s)	69, 619, 820, 900, 1088
SchI	GAGTC	6 cut(s)	859, 952, 1127, 1319, 1325, 1396
ScrFI	CCNGG	2 cut(s)	641, 737
SduI	GDGCHC	3 cut(s)	196, 244, 1259
SfaNI	GCATC	7 cut(s)	28, 48, 108, 342, 593, 762, 1135
SfcI	CTRYAG	3 cut(s)	46, 930, 1068
Sfr274I	CTCGAG	1 cut(s)	998
SlaI	CTCGAG	1 cut(s)	998
SmiMI	CAYNNNNRTG	1 cut(s)	234
SmlI	CTYRAG	6 cut(s)	272, 428, 475, 500, 539, 998
SmoI	CTYRAG	6 cut(s)	272, 428, 475, 500, 539, 998
Sse9I	AATT	2 cut(s)	203, 330
SseBI	AGGCCT	1 cut(s)	140
SsiI	CCGC	3 cut(s)	312, 516, 1149
SspMI	CTAG	1 cut(s)	147
SstI	GAGCTC	1 cut(s)	1259
StuI	AGGCCT	1 cut(s)	140
StyD4I	CCNGG	2 cut(s)	639, 735
StyI	CCWWGG	3 cut(s)	62, 141, 711
TaaI	ACNGT	4 cut(s)	32, 784, 863, 1099
TaqI	TCGA	6 cut(s)	126, 999, 1034, 1116, 1290, 1379
TasI	AATT	2 cut(s)	203, 330
TauI	GCSGC	2 cut(s)	518, 1151
TfiI	GAWTC	3 cut(s)	316, 433, 732
Tru1I	TTAA	8 cut(s)	117, 285, 501, 540, 894, 942, 1373, 1459
Tru9I	TTAA	8 cut(s)	117, 285, 501, 540, 894, 942, 1373, 1459
TscAI	CASTG	5 cut(s)	256, 305, 610, 698, 1132
TseFI	GTSAC	2 cut(s)	801, 1430
TseI	GCWGC	8 cut(s)	371, 551, 662, 719, 932, 1067, 1070, 1349
Tsp45I	GTSAC	2 cut(s)	801, 1430
TspDTI	ATGAA	4 cut(s)	271, 840, 897, 1232
TspGWI	ACGGA	1 cut(s)	716
TspRI	CASTG	5 cut(s)	256, 305, 610, 698, 1132
Tth111I	GACNNNGTC	1 cut(s)	686
Vha464I	CTTAAG	2 cut(s)	500, 539
XagI	CCTNNNNNAGG	3 cut(s)	46, 478, 998
XapI	RAATTY	1 cut(s)	203
XceI	RCATGY	1 cut(s)	378
XhoI	CTCGAG	1 cut(s)	998
XspI	CTAG	1 cut(s)	147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.