Prupe.3G151100_v2.0.a1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0009418
Physical Location & Seq
Forward (+)
16546777 .. 16549662
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G151100.1

Sequence Viewer

Length: 1317 bp
ATGGCTTTACTGAACATTGGTGCTGGGAACAGTGATGATGCCTTCTACAGGTATAAGATGCCCAAAATGGTTACCAAAATTGAGGGCCGAGGAAATGGCATCAAGACAAATATAGTCAACATGGTTGATATCGCAAAGGCTTTGGCAAGGCCCGCTGCGTACACTACAAAGTATTTTGGTTGTGAACTTGGCGCACAATCCAAGTTTGATGAGAAAACTGGGACTTCTATTGTTAATGGGTCCCATGACACTGCTAAGCTAGCTGGCCTCCTTGAAAACTTTATCAAGAAATTTGTTCAGTGTTATGGATGTGGAAATCCAGAAACAGAGATACTGATTACGAAGACTCAAATGATTCAGCTCAAATGTGCTGCTTGTGGTTTTGTGTCTGATGTGGACATGAGGGACAAGCTCACTACTTTCATTATTAAGAACCCACCTGAGCAGAAAAAGGGATCCAAAGACAAGAAAGCAATGAGAAGAGCTGAGAAGGAAAGACTAAAAGAAGGTGAAGCTGCTGATGAGGAGCTGAAAAAACTGAAGAAAGAGGCAAAGAAGAAGGGCACTTCCTCTGCTAAGGAGGGCCCTGTGAAGGCCAGCTCTTCAAAAAAGAAAACAAGTGCCTCTGATGAGGATCGTACATCACCTACTCCCAGCCAGGCTGATGAAGAGGAGGCTGATGATGATGATGATGATGTTCAGTGGCAAACTGATACGTCGATAGAGGCAGCTCGTCAACGGATGCAAGAACAGTTGAATGATGCGACAGCTGATATGGTGATGCTTCCCACCAATGAACCAGAGAAGGCCAAGGCAGAAACAAAGGCAAATGAGAGTTTGGAAAATGGGAACTCCACTGCTCGAGGAACACTTACGGATGAGTTGAAAAAGAATCTGGATAAGGGTATTTCACCCAAACAATTCCAATCCCTTCTGGGGTCACTTTCTGGGTCTGCAAAGGAAAAAATGACTGCTCTATTTGATGCGCTATTTGGGGGCCTAGAGAAAGGGTTTGCAAAAGAGGTATCCAAGAAGATAAACTACCTTGCTGTAGCTGCTGCTCAGGATGAGGAATCTCAATTGCTCTTGCTTCAAGCAATTGAAGGCTTCTGTGGGAAGTCGAGCTCAAGTGCCTTGAAGGAAGTTGCCCTTGTTTTAAAAGCTCTCTATGATGTTGATATCTTGGAGGAAGAATGCATAGTGCAGTGGTATCAAGAGGGACTGAAGGGTGGGAGCAAGAACTCTCAGATTTGGAAGAACGCCAAACCATTCATTGACTGGCTCCAGAGTGCTGAGTCCGAAACGGAGGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko04214 map03013 map04214
Pfam Domains
Protein Families
W2 eIF-5_eIF-2B

Protein Analysis

439

Amino Acids

48.03

Weight (kDa)

5.35

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 153
AclWI GGATC 3 cut(s) 450, 463, 642
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 2 cut(s) 560, 1244
AfaI GTAC 2 cut(s) 161, 640
AfiI CCNNNNNNNGG 3 cut(s) 48, 592, 936
AgsI TTSAA 7 cut(s) 275, 606, 757, 886, 1094, 1103, 1138
AjnI CCWGG 1 cut(s) 657
Alw21I GWGCWC 1 cut(s) 1127
AlwI GGATC 3 cut(s) 450, 463, 642
Ama87I CYCGRG 1 cut(s) 861
AoxI GGCC 7 cut(s) 85, 149, 265, 583, 594, 807, 997
ApaI GGGCCC 1 cut(s) 587
ApeKI GCWGC 6 cut(s) 155, 371, 515, 728, 1055, 1058
ApoI RAATTY 1 cut(s) 290
AspLEI GCGC 2 cut(s) 194, 988
AspS9I GGNCC 6 cut(s) 85, 150, 240, 583, 584, 997
AsuHPI GGTGA 4 cut(s) 521, 636, 790, 903
AsuNHI GCTAGC 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 1 cut(s) 240
BaeGI GKGCMC 2 cut(s) 566, 587
BamHI GGATCC 1 cut(s) 455
BanII GRGCYC 2 cut(s) 587, 1127
BarI GAAGNNNNNNTAC 2 cut(s) 1025, 1057
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 1127
BbvI GCAGC 6 cut(s) 142, 358, 502, 740, 1042, 1045
BciT130I CCWGG 1 cut(s) 659
BciVI GTATCC 1 cut(s) 1036
BfaI CTAG 2 cut(s) 260, 1001
BfmI CTRYAG 2 cut(s) 46, 1050
BfuI GTATCC 1 cut(s) 1036
BisI GCNGC 6 cut(s) 156, 372, 516, 729, 1056, 1059
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 6 cut(s) 157, 373, 517, 730, 1057, 1060
Bme1390I CCNGG 1 cut(s) 659
Bme18I GGWCC 1 cut(s) 240
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 6 cut(s) 85, 150, 240, 583, 584, 997
BmiI GGNNCC 6 cut(s) 241, 242, 457, 585, 998, 1283
BmrFI CCNGG 1 cut(s) 659
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 7 cut(s) 28, 48, 108, 732, 751, 771, 973
BmtI GCTAGC 1 cut(s) 263
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 350
BpmI CTGGAG 1 cut(s) 1268
Bpu10I CCTNAGC 3 cut(s) 441, 576, 1062
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 1111
BsaBI GATNNNNATC 1 cut(s) 633
BsaJI CCNNGG 2 cut(s) 88, 810
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 592, 936
Bse1I ACTGG 2 cut(s) 223, 1283
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 633
BseBI CCWGG 1 cut(s) 659
BseDI CCNNGG 2 cut(s) 88, 810
BseGI GGATG 4 cut(s) 314, 747, 883, 1072
BseJI GATNNNNATC 1 cut(s) 633
BseLI CCNNNNNNNGG 3 cut(s) 48, 592, 936
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 5 cut(s) 432, 477, 1076, 1259, 1284
BseNI ACTGG 2 cut(s) 223, 1283
BseRI GAGGAG 2 cut(s) 539, 686
BseSI GKGCMC 2 cut(s) 566, 587
BseXI GCAGC 6 cut(s) 142, 358, 502, 740, 1042, 1045
BseYI CCCAGC 2 cut(s) 23, 653
BsgI GTGCAG 1 cut(s) 1223
BshFI GGCC 7 cut(s) 87, 151, 267, 585, 596, 809, 999
BsiHKAI GWGCWC 1 cut(s) 1127
BsiHKCI CYCGRG 1 cut(s) 861
BslFI GGGAC 4 cut(s) 226, 235, 419, 1233
BslI CCNNNNNNNGG 3 cut(s) 48, 592, 936
BsmFI GGGAC 4 cut(s) 226, 235, 419, 1233
BsmI GAATGC 1 cut(s) 1199
BsnI GGCC 7 cut(s) 87, 151, 267, 585, 596, 809, 999
BsoBI CYCGRG 1 cut(s) 861
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 3 cut(s) 566, 587, 1127
Bsp143I GATC 2 cut(s) 455, 634
Bsp1720I GCTNAGC 1 cut(s) 255
BspACI CCGC 1 cut(s) 153
BspANI GGCC 7 cut(s) 87, 151, 267, 585, 596, 809, 999
BspCNI CTCAG 5 cut(s) 433, 478, 1075, 1258, 1285
BspLI GGNNCC 6 cut(s) 241, 242, 457, 585, 998, 1283
BspOI GCTAGC 1 cut(s) 263
BspPI GGATC 3 cut(s) 450, 463, 642
BspQI GCTCTTC 2 cut(s) 475, 607
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 2 cut(s) 223, 1283
BssECI CCNNGG 2 cut(s) 88, 810
BssMI GATC 2 cut(s) 455, 634
BssT1I CCWWGG 1 cut(s) 810
Bst2UI CCWGG 1 cut(s) 659
Bst4CI ACNGT 2 cut(s) 32, 753
Bst6I CTCTTC 3 cut(s) 475, 607, 663
BstC8I GCNNGC 4 cut(s) 153, 261, 265, 598
BstDEI CTNAG 7 cut(s) 255, 441, 486, 576, 1062, 1245, 1293
BstEII GGTNACC 1 cut(s) 70
BstENI CCTNNNNNAGG 1 cut(s) 46
BstF5I GGATG 4 cut(s) 314, 747, 883, 1072
BstHHI GCGC 2 cut(s) 194, 988
BstKTI GATC 2 cut(s) 458, 637
BstMBI GATC 2 cut(s) 455, 634
BstMWI GCNNNNNNNGC 3 cut(s) 152, 260, 1055
BstNI CCWGG 1 cut(s) 659
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 657
BstSFI CTRYAG 2 cut(s) 46, 1050
BstSLI GKGCMC 2 cut(s) 566, 587
BstV1I GCAGC 6 cut(s) 142, 358, 502, 740, 1042, 1045
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BsuI GTATCC 1 cut(s) 1036
BsuRI GGCC 7 cut(s) 87, 151, 267, 585, 596, 809, 999
BtsCI GGATG 4 cut(s) 314, 747, 883, 1072
BtsI GCAGTG 3 cut(s) 249, 855, 1211
BtsIMutI CAGTG 6 cut(s) 37, 249, 305, 707, 855, 1211
Cac8I GCNNGC 4 cut(s) 153, 261, 265, 598
CfoI GCGC 2 cut(s) 194, 988
Cfr13I GGNCC 6 cut(s) 85, 150, 240, 583, 584, 997
Csp6I GTAC 2 cut(s) 160, 639
CviAII CATG 3 cut(s) 121, 245, 400
CviQI GTAC 2 cut(s) 160, 639
DdeI CTNAG 7 cut(s) 255, 441, 486, 576, 1062, 1245, 1293
DpnI GATC 2 cut(s) 457, 636
DpnII GATC 2 cut(s) 455, 634
DraI TTTAAA 1 cut(s) 1158
Eam1104I CTCTTC 3 cut(s) 475, 607, 663
EarI CTCTTC 3 cut(s) 475, 607, 663
Ecl136II GAGCTC 1 cut(s) 1125
Eco130I CCWWGG 1 cut(s) 810
Eco24I GRGCYC 2 cut(s) 587, 1127
Eco32I GATATC 2 cut(s) 130, 1180
Eco47I GGWCC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 1125
Eco57I CTGAAG 2 cut(s) 560, 1244
Eco88I CYCGRG 1 cut(s) 861
Eco91I GGTNACC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 1125
EcoNI CCTNNNNNAGG 1 cut(s) 46
EcoO109I RGGNCCY 4 cut(s) 240, 583, 584, 997
EcoO65I GGTNACC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 657
EcoRV GATATC 2 cut(s) 130, 1180
EcoT14I CCWWGG 1 cut(s) 810
EcoT22I ATGCAT 1 cut(s) 1199
EcoT38I GRGCYC 2 cut(s) 587, 1127
ErhI CCWWGG 1 cut(s) 810
FaeI CATG 3 cut(s) 124, 248, 403
FaiI YATR 9 cut(s) 54, 113, 122, 246, 306, 401, 776, 1170, 1199
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FaqI GGGAC 4 cut(s) 226, 235, 419, 1233
FatI CATG 3 cut(s) 120, 244, 399
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 6 cut(s) 156, 372, 516, 729, 1056, 1059
FokI GGATG 4 cut(s) 321, 754, 890, 1079
FriOI GRGCYC 2 cut(s) 587, 1127
Fsp4HI GCNGC 6 cut(s) 156, 372, 516, 729, 1056, 1059
FspBI CTAG 2 cut(s) 260, 1001
GlaI GCGC 2 cut(s) 193, 987
GluI GCNGC 6 cut(s) 156, 372, 516, 729, 1056, 1059
GsaI CCCAGC 2 cut(s) 27, 657
GsuI CTGGAG 1 cut(s) 1268
HaeIII GGCC 7 cut(s) 87, 151, 267, 585, 596, 809, 999
HhaI GCGC 2 cut(s) 194, 988
Hin1II CATG 3 cut(s) 124, 248, 403
Hin6I GCGC 2 cut(s) 192, 986
HinP1I GCGC 2 cut(s) 192, 986
HincII GTYRAC 2 cut(s) 118, 737
HindII GTYRAC 2 cut(s) 118, 737
HinfI GANTC 5 cut(s) 346, 355, 892, 1073, 1295
HphI GGTGA 4 cut(s) 521, 636, 790, 903
Hpy166II GTNNAC 5 cut(s) 118, 162, 185, 397, 737
Hpy188I TCNGA 4 cut(s) 391, 628, 1248, 1300
Hpy188III TCNNGA 7 cut(s) 103, 286, 320, 896, 1064, 1214, 1285
Hpy8I GTNNAC 5 cut(s) 118, 162, 185, 397, 737
Hpy99I CGWCG 1 cut(s) 721
HpyCH4III ACNGT 2 cut(s) 32, 753
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 5 cut(s) 745, 956, 1016, 1197, 1204
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 260, 1055
HpyF3I CTNAG 7 cut(s) 255, 441, 486, 576, 1062, 1245, 1293
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 3 cut(s) 124, 248, 403
HspAI GCGC 2 cut(s) 192, 986
KflI GGGWCCC 1 cut(s) 240
Kzo9I GATC 2 cut(s) 455, 634
LguI GCTCTTC 2 cut(s) 475, 607
LmnI GCTCC 3 cut(s) 526, 1233, 1287
Lsp1109I GCAGC 6 cut(s) 142, 358, 502, 740, 1042, 1045
LweI GCATC 7 cut(s) 28, 48, 108, 732, 751, 771, 973
MaeI CTAG 2 cut(s) 260, 1001
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 2 cut(s) 70, 939
MalI GATC 2 cut(s) 457, 636
MboI GATC 2 cut(s) 455, 634
MboII GAAGA 9 cut(s) 355, 492, 553, 568, 594, 680, 1045, 1202, 1267
MfeI CAATTG 2 cut(s) 1079, 1098
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 3 cut(s) 566, 587, 1127
MluCI AATT 5 cut(s) 78, 290, 920, 1079, 1098
MlyI GAGTC 2 cut(s) 340, 1304
Mph1103I ATGCAT 1 cut(s) 1199
MseI TTAA 4 cut(s) 234, 429, 1157, 1315
MspA1I CMGCKG 2 cut(s) 155, 770
MspR9I CCNGG 1 cut(s) 659
MunI CAATTG 2 cut(s) 1079, 1098
Mva1269I GAATGC 1 cut(s) 1199
MvaI CCWGG 1 cut(s) 659
MwoI GCNNNNNNNGC 3 cut(s) 152, 260, 1055
NdeII GATC 2 cut(s) 455, 634
NheI GCTAGC 1 cut(s) 259
NlaIII CATG 3 cut(s) 124, 248, 403
NlaIV GGNNCC 6 cut(s) 241, 242, 457, 585, 998, 1283
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 1 cut(s) 939
NsiI ATGCAT 1 cut(s) 1199
PaeR7I CTCGAG 1 cut(s) 861
PciSI GCTCTTC 2 cut(s) 475, 607
PctI GAATGC 1 cut(s) 1199
PfeI GAWTC 3 cut(s) 355, 892, 1073
PkrI GCNGC 6 cut(s) 157, 373, 517, 730, 1057, 1060
PleI GAGTC 2 cut(s) 340, 1303
PpsI GAGTC 2 cut(s) 340, 1303
PpuMI RGGWCCY 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 1127
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 657
PspEI GGTNACC 1 cut(s) 70
PspFI CCCAGC 2 cut(s) 23, 653
PspGI CCWGG 1 cut(s) 657
PspN4I GGNNCC 6 cut(s) 241, 242, 457, 585, 998, 1283
PspOMI GGGCCC 1 cut(s) 583
PspPI GGNCC 6 cut(s) 85, 150, 240, 583, 584, 997
PspPPI RGGWCCY 1 cut(s) 240
PspXI VCTCGAGB 1 cut(s) 861
PsuI RGATCY 1 cut(s) 455
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 2 cut(s) 161, 640
RsaNI GTAC 2 cut(s) 160, 639
SacI GAGCTC 1 cut(s) 1127
SapI GCTCTTC 2 cut(s) 475, 607
SaqAI TTAA 4 cut(s) 234, 429, 1157, 1315
SatI GCNGC 6 cut(s) 156, 372, 516, 729, 1056, 1059
Sau3AI GATC 2 cut(s) 455, 634
Sau96I GGNCC 6 cut(s) 85, 150, 240, 583, 584, 997
SchI GAGTC 2 cut(s) 340, 1304
ScrFI CCNGG 1 cut(s) 659
SduI GDGCHC 3 cut(s) 566, 587, 1127
SfaNI GCATC 7 cut(s) 28, 48, 108, 732, 751, 771, 973
SfcI CTRYAG 2 cut(s) 46, 1050
Sfr274I CTCGAG 1 cut(s) 861
SinI GGWCC 1 cut(s) 240
SlaI CTCGAG 1 cut(s) 861
SmlI CTYRAG 2 cut(s) 861, 1126
SmoI CTYRAG 2 cut(s) 861, 1126
Sse9I AATT 5 cut(s) 78, 290, 920, 1079, 1098
SsiI CCGC 1 cut(s) 153
SspMI CTAG 2 cut(s) 260, 1001
SstI GAGCTC 1 cut(s) 1127
StyD4I CCNGG 1 cut(s) 657
StyI CCWWGG 1 cut(s) 810
TaaI ACNGT 2 cut(s) 32, 753
TaiI ACGT 1 cut(s) 719
TaqI TCGA 3 cut(s) 719, 862, 1121
TasI AATT 5 cut(s) 78, 290, 920, 1079, 1098
TfiI GAWTC 3 cut(s) 355, 892, 1073
Tru1I TTAA 4 cut(s) 234, 429, 1157, 1315
Tru9I TTAA 4 cut(s) 234, 429, 1157, 1315
TscAI CASTG 6 cut(s) 37, 256, 305, 707, 862, 1211
TseFI GTSAC 1 cut(s) 939
TseI GCWGC 6 cut(s) 155, 371, 515, 728, 1055, 1058
Tsp45I GTSAC 1 cut(s) 939
TspDTI ATGAA 4 cut(s) 412, 681, 810, 1261
TspGWI ACGGA 2 cut(s) 754, 890
TspRI CASTG 6 cut(s) 37, 256, 305, 707, 862, 1211
VpaK11BI GGWCC 1 cut(s) 240
XagI CCTNNNNNAGG 1 cut(s) 46
XapI RAATTY 1 cut(s) 290
XcmI CCANNNNNNNNNTGG 1 cut(s) 1275
XhoI CTCGAG 1 cut(s) 861
XspI CTAG 2 cut(s) 260, 1001
Zsp2I ATGCAT 1 cut(s) 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.