Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009418

Physical Location & Seq

Genomic Coordinates Forward (+)

1724245 .. 1725565

Gene Structure 1321 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00219650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGGCTCTACAGAACATTGGTGCTTCCAACCGTGATGATGCCTTCTACAGGTATAAGATGCCAAGGATGATAACTAAGATTGAAGGTAGAGGAAATGGCATCAAGACTAATGTGGTTAACATGGTGGAAATCGCAAAGGCCTTGGCTAGACCTGCTTCTTATACCACCAAGTATTTTGGCTGTGAGCTTGGAGCACAGTCAAAGTTCGATGAGAAGACTGGAACTTCCCATGTGAATGGTGCTCATGACACTGCAAAGCTTGCTGGACTTCTTGAGAACTTCATTAAGAAATATGTGCAGTGCTATGGTTGTGGGAACCCTGAAACTGAGATTATCATCACTAAGACTCAGATGCTCAATCTCAAATGTGCTGCATGTGGGTTTGTGTCTGATGTTGACATGAGGGACAAGCTTACAACTTTTATCCTCAAGAATCCTCCAGAGCAGAAGAAGTCTGCCAAAGAAAAGAAAGCCATGAGGAGGGCAGACAAAGAGCAGCTTAAGGAAGGGGAGGCTGCTGATGAGGAGCAAAAGAAGCTTAAGAAGGAGGCAGCAAGAAAGAAAGGCACTTCTGGCTCTAAAGTATCAAGCAAGAAGAAAACCGCCGGTTCTGATGAGGATCACTCCCCTACTCGGAGCCACGGAGATGTGATTGACCAAGCCGCTGAAGACGATGATGATGATGTCCAGTGGCAGACAGACACATCCATAGAGGCAGCCAAGAAGCGAATCGAGGAGCAGTTGAGTGCTGTTACTGCTGATATGAAGAGAAGAAAAAAATCTCCACCAAAGTCTCCAGAGCGTGAAAAGAAAGCTGAGGCCAAGAGTGAGAATGGTGCTGATAATGCCCATGGGAAGCTTGTTGATGAGATCAAACAATCCTTGAAAGCTGGGAGCAATAGCAGTCAGCTCAAAACCCTTTTGGGTACTCTTTCTGGTACTCCCCAAGAGAAAATGGATGCCTTACTTGAAGCCCTCTTTGAAGGTGTTGGAAAGGGGTTCTCCAAGGAGGTGACCAAGAAGATCAAGTACATTTCTGAAGCAACTATAGCCGAGGGCTCTCAGCTGACTTTGTTGCGTGGACTTGAGTCCTTCTGTGAGAAGGCGAACCCTGATGCTACAAAGGAGGTGGCTTTGGTTTTGAAATCACTGTATGATGAAGATGTGCTGGAGGAAGAGTACATTATGAAGTGGTATCAGCAAGGCTTAAGCGGCAAGGCAAAAAGCTCCCAGATTTGGAAGAACATTAAGCCTTTCATTGAGTGGCTCCAGAGTGCTGAGTCGGAGACAGAAGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03013 ko04214 map03013 map04214

Pfam Domains

Protein Families

W2 eIF-5_eIF-2B

Protein Analysis

433

Amino Acids

48.0

Weight (kDa)

8.25

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	11 - 127	1.7e-34	Domain found in IF2B/IF5
W2	PF02020	354 - 433	2.2e-22	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36730 AT1G77840
fragaria_vesca	FvH4_5g38780
malus_domestica	MD00G1104000.v1.1 MD09G1259500.v1.1 MD15G1359300.v1.1
prunus_persica	Prupe.1G506900_v2.0.a1 Prupe.3G151100_v2.0.a1
pyrus_communis	pycom08g14860 pycom09g17420 pycom15g32170
rosa_chinensis	RchiOBHm_Chr7g0240701
rosa_laevigata	RLG00000000625
rosa_multiflora	Rmu_sc0004799.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219650
rosa_rugosa	Rorug07G0338400 Rorug07G0338400
rosa_samantha	Rh7AG493000 Rh7BG465000 Rh7CG511400 Rh7DG478200
rosa_wichuraiana	Rw7G041700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	160
AciI	CCGC	3 cut(s)	603, 663, 1212
AclWI	GGATC	1 cut(s)	627
AcuI	CTGAAG	2 cut(s)	687, 1059
AfaI	GTAC	4 cut(s)	928, 940, 1031, 1181
AfiI	CCNNNNNNNGG	4 cut(s)	48, 480, 633, 1236
AflII	CTTAAG	3 cut(s)	500, 539, 1207
AgsI	TTSAA	5 cut(s)	83, 886, 971, 983, 1144
Alw21I	GWGCWC	2 cut(s)	196, 244
Alw26I	GTCTC	2 cut(s)	798, 1280
AlwI	GGATC	1 cut(s)	627
AoxI	GGCC	2 cut(s)	138, 819
ApeKI	GCWGC	5 cut(s)	371, 496, 515, 551, 716
AsuHPI	GGTGA	1 cut(s)	1024
BanII	GRGCYC	1 cut(s)	1061
BarI	GAAGNNNNNNTAC	2 cut(s)	1013, 1045
BbsI	GAAGAC	2 cut(s)	221, 675
Bbv12I	GWGCWC	2 cut(s)	196, 244
BbvCI	CCTCAGC	1 cut(s)	816
BbvI	GCAGC	5 cut(s)	358, 502, 508, 563, 728
BcoDI	GTCTC	2 cut(s)	798, 1280
BfaI	CTAG	1 cut(s)	147
BfmI	CTRYAG	3 cut(s)	8, 46, 1047
BfrI	CTTAAG	3 cut(s)	500, 539, 1207
BfuAI	ACCTGC	1 cut(s)	160
BisI	GCNGC	7 cut(s)	372, 497, 516, 552, 663, 717, 1213
BlsI	GCNGC	7 cut(s)	373, 498, 517, 553, 664, 718, 1214
BmiI	GGNNCC	3 cut(s)	317, 638, 1268
BmsI	GCATC	6 cut(s)	28, 48, 108, 342, 949, 1105
BoxI	GACNNNNGTC	1 cut(s)	1087
BpiI	GAAGAC	2 cut(s)	221, 675
BplI	GAGNNNNNCTC	2 cut(s)	608, 640
BpmI	CTGGAG	4 cut(s)	423, 780, 1190, 1253
Bpu10I	CCTNAGC	1 cut(s)	816
BpuEI	CTTGAG	3 cut(s)	293, 413, 1106
BsaBI	GATNNNNATC	2 cut(s)	335, 618
BsaJI	CCNNGG	6 cut(s)	62, 141, 640, 850, 1005, 1053
BsaXI	ACNNNNNCTCC	2 cut(s)	1163, 1193
Bsc4I	CCNNNNNNNGG	4 cut(s)	48, 480, 633, 1236
Bse118I	RCCGGY	1 cut(s)	605
Bse1I	ACTGG	2 cut(s)	223, 688
Bse8I	GATNNNNATC	2 cut(s)	335, 618
BseDI	CCNNGG	6 cut(s)	62, 141, 640, 850, 1005, 1053
BseGI	GGATG	3 cut(s)	72, 704, 964
BseJI	GATNNNNATC	2 cut(s)	335, 618
BseLI	CCNNNNNNNGG	4 cut(s)	48, 480, 633, 1236
BseMII	CTCAG	5 cut(s)	318, 362, 807, 1076, 1269
BseNI	ACTGG	2 cut(s)	223, 688
BseRI	GAGGAG	3 cut(s)	493, 539, 749
BseXI	GCAGC	5 cut(s)	358, 502, 508, 563, 728
BseYI	CCCAGC	1 cut(s)	890
BsgI	GTGCAG	1 cut(s)	317
BshFI	GGCC	2 cut(s)	140, 821
BsiHKAI	GWGCWC	2 cut(s)	196, 244
BsiSI	CCGG	1 cut(s)	606
BslFI	GGGAC	1 cut(s)	419
BslI	CCNNNNNNNGG	4 cut(s)	48, 480, 633, 1236
BsmAI	GTCTC	2 cut(s)	798, 1280
BsmFI	GGGAC	1 cut(s)	419
BsnI	GGCC	2 cut(s)	140, 821
Bsp1286I	GDGCHC	3 cut(s)	196, 244, 1061
Bsp143I	GATC	3 cut(s)	619, 870, 1023
Bsp19I	CCATGG	1 cut(s)	850
BspACI	CCGC	3 cut(s)	603, 663, 1212
BspANI	GGCC	2 cut(s)	140, 821
BspCNI	CTCAG	5 cut(s)	319, 361, 808, 1075, 1270
BspHI	TCATGA	1 cut(s)	244
BspLI	GGNNCC	3 cut(s)	317, 638, 1268
BspMI	ACCTGC	1 cut(s)	160
BspPI	GGATC	1 cut(s)	627
BspTI	CTTAAG	3 cut(s)	500, 539, 1207
BsrFI	RCCGGY	1 cut(s)	605
BsrI	ACTGG	2 cut(s)	223, 688
BssAI	RCCGGY	1 cut(s)	605
BssECI	CCNNGG	6 cut(s)	62, 141, 640, 850, 1005, 1053
BssMI	GATC	3 cut(s)	619, 870, 1023
BssT1I	CCWWGG	4 cut(s)	62, 141, 850, 1005
Bst4CI	ACNGT	3 cut(s)	32, 198, 1152
Bst6I	CTCTTC	3 cut(s)	761, 1170, 1287
BstAFI	CTTAAG	3 cut(s)	500, 539, 1207
BstAPI	GCANNNNNTGC	1 cut(s)	260
BstC8I	GCNNGC	1 cut(s)	261
BstDEI	CTNAG	7 cut(s)	75, 327, 342, 348, 816, 1062, 1278
BstDSI	CCRYGG	2 cut(s)	640, 850
BstEII	GGTNACC	1 cut(s)	1012
BstENI	CCTNNNNNAGG	1 cut(s)	46
BstF5I	GGATG	3 cut(s)	72, 704, 964
BstKTI	GATC	3 cut(s)	622, 873, 1026
BstMAI	GTCTC	2 cut(s)	798, 1280
BstMBI	GATC	3 cut(s)	619, 870, 1023
BstMWI	GCNNNNNNNGC	8 cut(s)	152, 260, 535, 573, 755, 845, 1049, 1212
BstNSI	RCATGY	1 cut(s)	378
BstPAI	GACNNNNGTC	1 cut(s)	1087
BstPI	GGTNACC	1 cut(s)	1012
BstSFI	CTRYAG	3 cut(s)	8, 46, 1047
BstV1I	GCAGC	5 cut(s)	358, 502, 508, 563, 728
BstV2I	GAAGAC	2 cut(s)	221, 675
BstXI	CCANNNNNNTGG	1 cut(s)	236
BsuRI	GGCC	2 cut(s)	140, 821
BtgI	CCRYGG	2 cut(s)	640, 850
BtsCI	GGATG	3 cut(s)	72, 704, 964
BtsI	GCAGTG	2 cut(s)	249, 305
BtsIMutI	CAGTG	4 cut(s)	249, 305, 695, 1148
BveI	ACCTGC	1 cut(s)	160
Cac8I	GCNNGC	1 cut(s)	261
CciI	TCATGA	1 cut(s)	244
Cfr10I	RCCGGY	1 cut(s)	605
Csp6I	GTAC	4 cut(s)	927, 939, 1030, 1180
CspCI	CAANNNNNGTGG	2 cut(s)	1110, 1145
CviAII	CATG	7 cut(s)	121, 230, 245, 375, 400, 475, 851
CviQI	GTAC	4 cut(s)	927, 939, 1030, 1180
DdeI	CTNAG	7 cut(s)	75, 327, 342, 348, 816, 1062, 1278
DpnI	GATC	3 cut(s)	621, 872, 1025
DpnII	GATC	3 cut(s)	619, 870, 1023
Eam1104I	CTCTTC	3 cut(s)	761, 1170, 1287
EarI	CTCTTC	3 cut(s)	761, 1170, 1287
Eco130I	CCWWGG	4 cut(s)	62, 141, 850, 1005
Eco147I	AGGCCT	1 cut(s)	140
Eco24I	GRGCYC	1 cut(s)	1061
Eco57I	CTGAAG	2 cut(s)	687, 1059
Eco91I	GGTNACC	1 cut(s)	1012
EcoNI	CCTNNNNNAGG	1 cut(s)	46
EcoO65I	GGTNACC	1 cut(s)	1012
EcoT14I	CCWWGG	4 cut(s)	62, 141, 850, 1005
EcoT38I	GRGCYC	1 cut(s)	1061
ErhI	CCWWGG	4 cut(s)	62, 141, 850, 1005
FaeI	CATG	7 cut(s)	124, 233, 248, 378, 403, 478, 854
FalI	AAGNNNNNCTT	2 cut(s)	483, 515
FaqI	GGGAC	1 cut(s)	419
FatI	CATG	7 cut(s)	120, 229, 244, 374, 399, 474, 850
Fnu4HI	GCNGC	7 cut(s)	372, 497, 516, 552, 663, 717, 1213
FokI	GGATG	3 cut(s)	79, 691, 971
FriOI	GRGCYC	1 cut(s)	1061
Fsp4HI	GCNGC	7 cut(s)	372, 497, 516, 552, 663, 717, 1213
FspBI	CTAG	1 cut(s)	147
GluI	GCNGC	7 cut(s)	372, 497, 516, 552, 663, 717, 1213
GsaI	CCCAGC	1 cut(s)	894
GsuI	CTGGAG	4 cut(s)	423, 780, 1190, 1253
HaeIII	GGCC	2 cut(s)	140, 821
HapII	CCGG	1 cut(s)	606
Hin1II	CATG	7 cut(s)	124, 233, 248, 378, 403, 478, 854
HincII	GTYRAC	2 cut(s)	118, 397
HindII	GTYRAC	2 cut(s)	118, 397
HindIII	AAGCTT	4 cut(s)	257, 410, 536, 857
HinfI	GANTC	5 cut(s)	346, 433, 729, 1088, 1280
HpaI	GTTAAC	1 cut(s)	118
HpaII	CCGG	1 cut(s)	606
HphI	GGTGA	1 cut(s)	1024
Hpy166II	GTNNAC	3 cut(s)	118, 397, 1082
Hpy188I	TCNGA	6 cut(s)	351, 391, 613, 636, 1039, 1285
Hpy188III	TCNNGA	7 cut(s)	103, 245, 272, 430, 440, 797, 1270
Hpy8I	GTNNAC	3 cut(s)	118, 397, 1082
HpyAV	CCTTC	7 cut(s)	52, 77, 500, 538, 977, 1096, 1102
HpyCH4III	ACNGT	3 cut(s)	32, 198, 1152
HpyCH4V	TGCA	3 cut(s)	254, 298, 374
HpyF10VI	GCNNNNNNNGC	8 cut(s)	152, 260, 535, 573, 755, 845, 1049, 1212
HpyF3I	CTNAG	7 cut(s)	75, 327, 342, 348, 816, 1062, 1278
Hsp92II	CATG	7 cut(s)	124, 233, 248, 378, 403, 478, 854
KspAI	GTTAAC	1 cut(s)	118
Kzo9I	GATC	3 cut(s)	619, 870, 1023
LmnI	GCTCC	7 cut(s)	191, 526, 636, 736, 894, 1232, 1272
Lsp1109I	GCAGC	5 cut(s)	358, 502, 508, 563, 728
LweI	GCATC	6 cut(s)	28, 48, 108, 342, 949, 1105
MaeI	CTAG	1 cut(s)	147
MaeIII	GTNAC	2 cut(s)	751, 1012
MalI	GATC	3 cut(s)	621, 872, 1025
MboI	GATC	3 cut(s)	619, 870, 1023
MhlI	GDGCHC	3 cut(s)	196, 244, 1061
MlyI	GAGTC	3 cut(s)	340, 1097, 1289
MmeI	TCCRAC	3 cut(s)	51, 970, 1263
MseI	TTAA	6 cut(s)	117, 285, 501, 540, 1208, 1248
MslI	CAYNNNNRTG	2 cut(s)	234, 645
MspA1I	CMGCKG	2 cut(s)	665, 1066
MspCI	CTTAAG	3 cut(s)	500, 539, 1207
MspI	CCGG	1 cut(s)	606
MwoI	GCNNNNNNNGC	8 cut(s)	152, 260, 535, 573, 755, 845, 1049, 1212
NcoI	CCATGG	1 cut(s)	850
NdeII	GATC	3 cut(s)	619, 870, 1023
NlaIII	CATG	7 cut(s)	124, 233, 248, 378, 403, 478, 854
NlaIV	GGNNCC	3 cut(s)	317, 638, 1268
NmeAIII	GCCGAG	1 cut(s)	1078
NmuCI	GTSAC	1 cut(s)	1012
NspI	RCATGY	1 cut(s)	378
PagI	TCATGA	1 cut(s)	244
PceI	AGGCCT	1 cut(s)	140
PfeI	GAWTC	2 cut(s)	433, 729
PkrI	GCNGC	7 cut(s)	373, 498, 517, 553, 664, 718, 1214
PleI	GAGTC	3 cut(s)	340, 1096, 1288
PpsI	GAGTC	3 cut(s)	340, 1096, 1288
PshAI	GACNNNNGTC	1 cut(s)	1087
PspEI	GGTNACC	1 cut(s)	1012
PspFI	CCCAGC	1 cut(s)	890
PspN4I	GGNNCC	3 cut(s)	317, 638, 1268
PvuII	CAGCTG	1 cut(s)	1066
RsaI	GTAC	4 cut(s)	928, 940, 1031, 1181
RsaNI	GTAC	4 cut(s)	927, 939, 1030, 1180
RseI	CAYNNNNRTG	2 cut(s)	234, 645
SaqAI	TTAA	6 cut(s)	117, 285, 501, 540, 1208, 1248
SatI	GCNGC	7 cut(s)	372, 497, 516, 552, 663, 717, 1213
Sau3AI	GATC	3 cut(s)	619, 870, 1023
SchI	GAGTC	3 cut(s)	340, 1097, 1289
SduI	GDGCHC	3 cut(s)	196, 244, 1061
SfaNI	GCATC	6 cut(s)	28, 48, 108, 342, 949, 1105
SfcI	CTRYAG	3 cut(s)	8, 46, 1047
SmiMI	CAYNNNNRTG	2 cut(s)	234, 645
SmlI	CTYRAG	6 cut(s)	272, 428, 500, 539, 1085, 1207
SmoI	CTYRAG	6 cut(s)	272, 428, 500, 539, 1085, 1207
SseBI	AGGCCT	1 cut(s)	140
SsiI	CCGC	3 cut(s)	603, 663, 1212
SspMI	CTAG	1 cut(s)	147
StuI	AGGCCT	1 cut(s)	140
StyI	CCWWGG	4 cut(s)	62, 141, 850, 1005
TaaI	ACNGT	3 cut(s)	32, 198, 1152
TaqI	TCGA	2 cut(s)	207, 732
TatI	WGTACW	2 cut(s)	1029, 1179
TauI	GCSGC	2 cut(s)	665, 1215
TfiI	GAWTC	2 cut(s)	433, 729
Tru1I	TTAA	6 cut(s)	117, 285, 501, 540, 1208, 1248
Tru9I	TTAA	6 cut(s)	117, 285, 501, 540, 1208, 1248
TscAI	CASTG	4 cut(s)	256, 305, 695, 1155
TseFI	GTSAC	1 cut(s)	1012
TseI	GCWGC	5 cut(s)	371, 496, 515, 551, 716
Tsp45I	GTSAC	1 cut(s)	1012
TspDTI	ATGAA	5 cut(s)	271, 779, 1173, 1202, 1246
TspGWI	ACGGA	1 cut(s)	657
TspRI	CASTG	4 cut(s)	256, 305, 695, 1155
Vha464I	CTTAAG	3 cut(s)	500, 539, 1207
XagI	CCTNNNNNAGG	1 cut(s)	46
XceI	RCATGY	1 cut(s)	378
XspI	CTAG	1 cut(s)	147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.