Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0009418

Physical Location & Seq

Genomic Coordinates Reverse (-)

28679476 .. 28682309

Gene Structure 2834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g38780.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGCTCTACAGAACATTGGTGCTTCCAACCGTGACGATGCCTTCTATAGGTATAAGATGCCAAGGATGATAACAAAGATAGAAGGCAGAGGAAACGGCATCAAGACAAACGTGGTTAATATGGTGGAAATTGCGAAGGCCTTGGCCAGACCTGCTTCGTATACCACCAAGTATTTTGGTTGTGAGCTTGGAGCCCAGTCAAAGTTTGATGAGAAGACTGGAACGTCCCATGTTAATGGAGCTCATGACACTGCTAAGCTTGCTGGACTTCTTGAGAACTTCATTAAGAAGTATGTGCAGTGCTATGGCTGTGGGAACCCGGAGACTGAGATTATCATCACTAAGACACAGATGCTGAATCTTAAATGTGCAGCCTGTGGATTTGTGTCTGATGTTGACATGAGGGACAAGCTTACGACTTTTATCCTTAAGAATCCTCCTGAGCAGAAGAAATCTGCCAAAGAAAAGAAAGCCCTGAGGAGAGCAGACAAAGAGCAGCTCAAGGAAGGGCAAATTGCTGATGAGGAGCAAAAGAAACTTAAGAAGGAAGCGGCGAAAAAGAAAGGCACCTCCGGCTCCAAAGTATCAAGCAAGAAGAGGACCTCCGGTTCTGATGAAGATCACTCCCCTACTAGGAGCCAGGGAGATGGGATGGACCAAGCTGCTGATGACGATGATGATGTCCAGTGGCAGACAGACACATCCATTGAGGCAGCAAAGAAGCGAATCGAGGAGCAGTTGAGTGCTGTTACTGCTGATATGGTCATGCTTAATACCACTGAAGAGAAGAAAAAGTCCCCACCAAAGTCTCCAGAGCGTGAAAAGAAAGCAGAAGCCAAGAGTGAGAATGGTGTTGATGATGTCCATGGGAAGCTTGTTGATGAGATCAAGCAATCCTTGAAGGCAGGGAGCAGTGCCAGTCAGCTCAAAACCCTTTTCGGTACTCTGTGTGGTACTCCCCAAGAGAAAATGGATGCCTTACTTGAAGCCCTCTTTGAAGGTGTTGGAAAGGGGTTTGCCAAGGAGGTGAACAAGAAGATCAAGTACATCTCTGAAGCAACCAAAGCCGAGGGCTCTCAGCTGGTTTTGTTGCGTGGATTTGAGTCCTTCTGTGGTAAGGCAAACCCTGATGCTACAAAGGAGGTGGCTTTGGTTTTGAAATCGCTGTACGATGAAGATGTGCTGGAGGAGGAATACATCATGAAGTGGTATCAGGAAGGGTTAAGTGGCAAGGCTAAAAGCTCCCCTATTTGGAAGAACATTAAACCTTTCATTGAGTGGCTCCAGAGCGCTGAGTCCGAGACAGAAGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03013 ko04214 map03013 map04214

Pfam Domains

Protein Families

W2 eIF-5_eIF-2B

Protein Analysis

439

Amino Acids

48.49

Weight (kDa)

7.49

Isoelectric Point (pI)

41.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	11 - 127	1.8e-34	Domain found in IF2B/IF5
W2	PF02020	360 - 438	1.8e-21	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36730 AT1G77840
fragaria_vesca	FvH4_5g38780
malus_domestica	MD00G1104000.v1.1 MD09G1259500.v1.1 MD15G1359300.v1.1
prunus_persica	Prupe.1G506900_v2.0.a1 Prupe.3G151100_v2.0.a1
pyrus_communis	pycom08g14860 pycom09g17420 pycom15g32170
rosa_chinensis	RchiOBHm_Chr7g0240701
rosa_laevigata	RLG00000000625
rosa_multiflora	Rmu_sc0004799.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219650
rosa_rugosa	Rorug07G0338400 Rorug07G0338400
rosa_samantha	Rh7AG493000 Rh7BG465000 Rh7CG511400 Rh7DG478200
rosa_wichuraiana	Rw7G041700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	223
Acc36I	ACCTGC	1 cut(s)	160
AccB1I	GGYRCC	1 cut(s)	566
AccI	GTMKAC	1 cut(s)	161
AciI	CCGC	1 cut(s)	551
AcoI	YGGCCR	1 cut(s)	144
AcuI	CTGAAG	2 cut(s)	801, 1074
AfaI	GTAC	4 cut(s)	943, 955, 1046, 1169
AfeI	AGCGCT	1 cut(s)	1291
AfiI	CCNNNNNNNGG	3 cut(s)	48, 633, 1251
AflII	CTTAAG	2 cut(s)	428, 539
AgsI	TTSAA	4 cut(s)	901, 986, 998, 1159
AjnI	CCWGG	1 cut(s)	639
AloI	GAACNNNNNNTCC	2 cut(s)	592, 624
Alw21I	GWGCWC	1 cut(s)	244
Alw26I	GTCTC	3 cut(s)	317, 813, 1295
AlwNI	CAGNNNCTG	1 cut(s)	355
Aor51HI	AGCGCT	1 cut(s)	1291
AoxI	GGCC	2 cut(s)	138, 144
ApeKI	GCWGC	4 cut(s)	371, 496, 662, 713
AspLEI	GCGC	1 cut(s)	1292
AspS9I	GGNCC	2 cut(s)	600, 655
AsuC2I	CCSGG	1 cut(s)	320
AsuHPI	GGTGA	1 cut(s)	1039
AvaII	GGWCC	2 cut(s)	600, 655
AxyI	CCTNAGG	1 cut(s)	476
BalI	TGGCCA	1 cut(s)	146
BanI	GGYRCC	1 cut(s)	566
BanII	GRGCYC	3 cut(s)	196, 244, 1076
BarI	GAAGNNNNNNTAC	2 cut(s)	1028, 1060
BbsI	GAAGAC	1 cut(s)	221
Bbv12I	GWGCWC	1 cut(s)	244
BbvI	GCAGC	4 cut(s)	383, 508, 649, 725
BccI	CCATC	2 cut(s)	641, 646
BceAI	ACGGC	1 cut(s)	112
BciT130I	CCWGG	1 cut(s)	641
BcnI	CCSGG	1 cut(s)	320
BcoDI	GTCTC	3 cut(s)	317, 813, 1295
BfaI	CTAG	1 cut(s)	633
BfmI	CTRYAG	2 cut(s)	8, 46
BfoI	RGCGCY	1 cut(s)	1293
BfrI	CTTAAG	2 cut(s)	428, 539
BfuAI	ACCTGC	1 cut(s)	160
BisI	GCNGC	5 cut(s)	372, 497, 552, 663, 714
BlpI	GCTNAGC	1 cut(s)	255
BlsI	GCNGC	5 cut(s)	373, 498, 553, 664, 715
Bme1390I	CCNGG	2 cut(s)	320, 641
Bme18I	GGWCC	2 cut(s)	600, 655
BmgT120I	GGNCC	2 cut(s)	600, 655
BmiI	GGNNCC	6 cut(s)	193, 317, 568, 577, 638, 1283
BmrFI	CCNGG	2 cut(s)	320, 641
BmrI	ACTGGG	1 cut(s)	190
BmsI	GCATC	6 cut(s)	28, 48, 108, 342, 964, 1120
BmuI	ACTGGG	1 cut(s)	190
BpiI	GAAGAC	1 cut(s)	221
BpmI	CTGGAG	3 cut(s)	795, 1205, 1268
Bpu10I	CCTNAGC	1 cut(s)	441
Bpu1102I	GCTNAGC	1 cut(s)	255
BpuEI	CTTGAG	2 cut(s)	293, 485
BpuMI	CCSGG	1 cut(s)	320
BsaBI	GATNNNNATC	2 cut(s)	335, 618
BsaJI	CCNNGG	6 cut(s)	62, 141, 640, 865, 1020, 1068
BsaWI	WCCGGW	1 cut(s)	605
Bsc4I	CCNNNNNNNGG	3 cut(s)	48, 633, 1251
Bse1I	ACTGG	4 cut(s)	196, 223, 685, 918
Bse21I	CCTNAGG	1 cut(s)	476
Bse8I	GATNNNNATC	2 cut(s)	335, 618
BseBI	CCWGG	1 cut(s)	641
BseDI	CCNNGG	6 cut(s)	62, 141, 640, 865, 1020, 1068
BseGI	GGATG	4 cut(s)	72, 657, 701, 979
BseJI	GATNNNNATC	2 cut(s)	335, 618
BseLI	CCNNNNNNNGG	3 cut(s)	48, 633, 1251
BseMII	CTCAG	5 cut(s)	318, 432, 467, 1091, 1284
BseNI	ACTGG	4 cut(s)	196, 223, 685, 918
BseRI	GAGGAG	4 cut(s)	493, 539, 746, 1202
BseXI	GCAGC	4 cut(s)	383, 508, 649, 725
BsgI	GTGCAG	2 cut(s)	317, 390
BshFI	GGCC	2 cut(s)	140, 146
BshNI	GGYRCC	1 cut(s)	566
BsiHKAI	GWGCWC	1 cut(s)	244
BsiSI	CCGG	3 cut(s)	320, 573, 606
BslFI	GGGAC	3 cut(s)	211, 419, 781
BslI	CCNNNNNNNGG	3 cut(s)	48, 633, 1251
BsmAI	GTCTC	3 cut(s)	317, 813, 1295
BsmFI	GGGAC	3 cut(s)	211, 419, 781
BsnI	GGCC	2 cut(s)	140, 146
Bsp1286I	GDGCHC	3 cut(s)	196, 244, 1076
Bsp143I	GATC	3 cut(s)	619, 885, 1038
Bsp1720I	GCTNAGC	1 cut(s)	255
Bsp19I	CCATGG	1 cut(s)	865
BspACI	CCGC	1 cut(s)	551
BspANI	GGCC	2 cut(s)	140, 146
BspCNI	CTCAG	5 cut(s)	319, 433, 468, 1090, 1285
BspHI	TCATGA	2 cut(s)	244, 1200
BspLI	GGNNCC	6 cut(s)	193, 317, 568, 577, 638, 1283
BspMI	ACCTGC	1 cut(s)	160
BspT107I	GGYRCC	1 cut(s)	566
BspTI	CTTAAG	2 cut(s)	428, 539
BsrI	ACTGG	4 cut(s)	196, 223, 685, 918
BssECI	CCNNGG	6 cut(s)	62, 141, 640, 865, 1020, 1068
BssMI	GATC	3 cut(s)	619, 885, 1038
BssNAI	GTATAC	1 cut(s)	162
BssT1I	CCWWGG	4 cut(s)	62, 141, 865, 1020
Bst1107I	GTATAC	1 cut(s)	162
Bst2UI	CCWGG	1 cut(s)	641
Bst4CI	ACNGT	1 cut(s)	32
Bst6I	CTCTTC	3 cut(s)	590, 777, 1302
BstAFI	CTTAAG	2 cut(s)	428, 539
BstC8I	GCNNGC	1 cut(s)	261
BstDEI	CTNAG	7 cut(s)	255, 327, 342, 441, 476, 1077, 1293
BstDSI	CCRYGG	1 cut(s)	865
BstENI	CCTNNNNNAGG	1 cut(s)	46
BstF5I	GGATG	4 cut(s)	72, 657, 701, 979
BstH2I	RGCGCY	1 cut(s)	1293
BstHHI	GCGC	1 cut(s)	1292
BstKTI	GATC	3 cut(s)	622, 888, 1041
BstMAI	GTCTC	3 cut(s)	317, 813, 1295
BstMBI	GATC	3 cut(s)	619, 885, 1038
BstMWI	GCNNNNNNNGC	5 cut(s)	152, 260, 573, 752, 1064
BstNI	CCWGG	1 cut(s)	641
BstSCI	CCNGG	2 cut(s)	318, 639
BstSFI	CTRYAG	2 cut(s)	8, 46
BstV1I	GCAGC	4 cut(s)	383, 508, 649, 725
BstV2I	GAAGAC	1 cut(s)	221
BstXI	CCANNNNNNTGG	2 cut(s)	236, 647
BstZ17I	GTATAC	1 cut(s)	162
Bsu36I	CCTNAGG	1 cut(s)	476
BsuRI	GGCC	2 cut(s)	140, 146
BtgI	CCRYGG	1 cut(s)	865
BtsCI	GGATG	4 cut(s)	72, 657, 701, 979
BtsI	GCAGTG	3 cut(s)	249, 305, 919
BtsIMutI	CAGTG	5 cut(s)	249, 305, 692, 777, 919
BveI	ACCTGC	1 cut(s)	160
Cac8I	GCNNGC	1 cut(s)	261
CaiI	CAGNNNCTG	1 cut(s)	355
CciI	TCATGA	2 cut(s)	244, 1200
CfoI	GCGC	1 cut(s)	1292
Cfr13I	GGNCC	2 cut(s)	600, 655
Csp6I	GTAC	4 cut(s)	942, 954, 1045, 1168
CspCI	CAANNNNNGTGG	2 cut(s)	1125, 1160
CviAII	CATG	6 cut(s)	230, 245, 400, 766, 866, 1201
CviQI	GTAC	4 cut(s)	942, 954, 1045, 1168
DdeI	CTNAG	7 cut(s)	255, 327, 342, 441, 476, 1077, 1293
DpnI	GATC	3 cut(s)	621, 887, 1040
DpnII	GATC	3 cut(s)	619, 885, 1038
DrdI	GACNNNNNNGTC	1 cut(s)	223
DseDI	GACNNNNNNGTC	1 cut(s)	223
EaeI	YGGCCR	1 cut(s)	144
Eam1104I	CTCTTC	3 cut(s)	590, 777, 1302
EarI	CTCTTC	3 cut(s)	590, 777, 1302
Ecl136II	GAGCTC	1 cut(s)	242
Eco130I	CCWWGG	4 cut(s)	62, 141, 865, 1020
Eco147I	AGGCCT	1 cut(s)	140
Eco24I	GRGCYC	3 cut(s)	196, 244, 1076
Eco47I	GGWCC	2 cut(s)	600, 655
Eco47III	AGCGCT	1 cut(s)	1291
Eco53kI	GAGCTC	1 cut(s)	242
Eco57I	CTGAAG	2 cut(s)	801, 1074
Eco81I	CCTNAGG	1 cut(s)	476
EcoICRI	GAGCTC	1 cut(s)	242
EcoNI	CCTNNNNNAGG	1 cut(s)	46
EcoO109I	RGGNCCY	1 cut(s)	600
EcoRII	CCWGG	1 cut(s)	639
EcoT14I	CCWWGG	4 cut(s)	62, 141, 865, 1020
EcoT38I	GRGCYC	3 cut(s)	196, 244, 1076
ErhI	CCWWGG	4 cut(s)	62, 141, 865, 1020
FaeI	CATG	6 cut(s)	233, 248, 403, 769, 869, 1204
FalI	AAGNNNNNCTT	2 cut(s)	881, 913
FaqI	GGGAC	3 cut(s)	211, 419, 781
FatI	CATG	6 cut(s)	229, 244, 399, 765, 865, 1200
FblI	GTMKAC	1 cut(s)	161
Fnu4HI	GCNGC	5 cut(s)	372, 497, 552, 663, 714
FokI	GGATG	4 cut(s)	79, 664, 688, 986
FriOI	GRGCYC	3 cut(s)	196, 244, 1076
Fsp4HI	GCNGC	5 cut(s)	372, 497, 552, 663, 714
FspBI	CTAG	1 cut(s)	633
GlaI	GCGC	1 cut(s)	1291
GluI	GCNGC	5 cut(s)	372, 497, 552, 663, 714
GsuI	CTGGAG	3 cut(s)	795, 1205, 1268
HaeII	RGCGCY	1 cut(s)	1293
HaeIII	GGCC	2 cut(s)	140, 146
HapII	CCGG	3 cut(s)	320, 573, 606
HhaI	GCGC	1 cut(s)	1292
Hin1II	CATG	6 cut(s)	233, 248, 403, 769, 869, 1204
Hin6I	GCGC	1 cut(s)	1290
HinP1I	GCGC	1 cut(s)	1290
HincII	GTYRAC	1 cut(s)	397
HindII	GTYRAC	1 cut(s)	397
HindIII	AAGCTT	3 cut(s)	257, 410, 872
HinfI	GANTC	5 cut(s)	358, 433, 726, 1103, 1295
HpaII	CCGG	3 cut(s)	320, 573, 606
HphI	GGTGA	1 cut(s)	1039
Hpy166II	GTNNAC	3 cut(s)	162, 397, 1030
Hpy188I	TCNGA	4 cut(s)	391, 613, 1054, 1300
Hpy188III	TCNNGA	8 cut(s)	103, 245, 272, 440, 812, 1201, 1214, 1285
Hpy8I	GTNNAC	3 cut(s)	162, 397, 1030
HpyAV	CCTTC	9 cut(s)	52, 77, 130, 500, 538, 895, 992, 1117, 1211
HpyCH4III	ACNGT	1 cut(s)	32
HpyCH4IV	ACGT	2 cut(s)	111, 224
HpyCH4V	TGCA	2 cut(s)	298, 371
HpyF10VI	GCNNNNNNNGC	5 cut(s)	152, 260, 573, 752, 1064
HpyF3I	CTNAG	7 cut(s)	255, 327, 342, 441, 476, 1077, 1293
HpySE526I	ACGT	2 cut(s)	111, 224
Hsp92II	CATG	6 cut(s)	233, 248, 403, 769, 869, 1204
HspAI	GCGC	1 cut(s)	1290
Kzo9I	GATC	3 cut(s)	619, 885, 1038
LmnI	GCTCC	9 cut(s)	191, 239, 526, 581, 636, 733, 909, 1247, 1287
Lsp1109I	GCAGC	4 cut(s)	383, 508, 649, 725
LweI	GCATC	6 cut(s)	28, 48, 108, 342, 964, 1120
MaeI	CTAG	1 cut(s)	633
MaeII	ACGT	2 cut(s)	111, 224
MaeIII	GTNAC	2 cut(s)	32, 748
MalI	GATC	3 cut(s)	621, 887, 1040
MboI	GATC	3 cut(s)	619, 885, 1038
MboII	GAAGA	9 cut(s)	226, 460, 607, 629, 794, 799, 1048, 1187, 1267
MhlI	GDGCHC	3 cut(s)	196, 244, 1076
MlsI	TGGCCA	1 cut(s)	146
MluCI	AATT	2 cut(s)	129, 513
MluNI	TGGCCA	1 cut(s)	146
MlyI	GAGTC	2 cut(s)	1112, 1304
MmeI	TCCRAC	2 cut(s)	51, 985
Mox20I	TGGCCA	1 cut(s)	146
MscI	TGGCCA	1 cut(s)	146
MseI	TTAA	9 cut(s)	117, 234, 285, 363, 429, 540, 771, 1223, 1263
MslI	CAYNNNNRTG	1 cut(s)	234
Msp20I	TGGCCA	1 cut(s)	146
MspA1I	CMGCKG	1 cut(s)	1081
MspCI	CTTAAG	2 cut(s)	428, 539
MspI	CCGG	3 cut(s)	320, 573, 606
MspR9I	CCNGG	2 cut(s)	320, 641
MvaI	CCWGG	1 cut(s)	641
MwoI	GCNNNNNNNGC	5 cut(s)	152, 260, 573, 752, 1064
NciI	CCSGG	1 cut(s)	320
NcoI	CCATGG	1 cut(s)	865
NdeII	GATC	3 cut(s)	619, 885, 1038
NlaIII	CATG	6 cut(s)	233, 248, 403, 769, 869, 1204
NlaIV	GGNNCC	6 cut(s)	193, 317, 568, 577, 638, 1283
NmeAIII	GCCGAG	1 cut(s)	1093
NmuCI	GTSAC	1 cut(s)	32
PagI	TCATGA	2 cut(s)	244, 1200
PceI	AGGCCT	1 cut(s)	140
PfeI	GAWTC	3 cut(s)	358, 433, 726
PkrI	GCNGC	5 cut(s)	373, 498, 553, 664, 715
PleI	GAGTC	2 cut(s)	1111, 1303
PpsI	GAGTC	2 cut(s)	1111, 1303
PpuMI	RGGWCCY	1 cut(s)	600
Psp124BI	GAGCTC	1 cut(s)	244
Psp5II	RGGWCCY	1 cut(s)	600
Psp6I	CCWGG	1 cut(s)	639
PspGI	CCWGG	1 cut(s)	639
PspN4I	GGNNCC	6 cut(s)	193, 317, 568, 577, 638, 1283
PspPI	GGNCC	2 cut(s)	600, 655
PspPPI	RGGWCCY	1 cut(s)	600
PstNI	CAGNNNCTG	1 cut(s)	355
PvuII	CAGCTG	1 cut(s)	1081
RsaI	GTAC	4 cut(s)	943, 955, 1046, 1169
RsaNI	GTAC	4 cut(s)	942, 954, 1045, 1168
RseI	CAYNNNNRTG	1 cut(s)	234
SacI	GAGCTC	1 cut(s)	244
SaqAI	TTAA	9 cut(s)	117, 234, 285, 363, 429, 540, 771, 1223, 1263
SatI	GCNGC	5 cut(s)	372, 497, 552, 663, 714
Sau3AI	GATC	3 cut(s)	619, 885, 1038
Sau96I	GGNCC	2 cut(s)	600, 655
SchI	GAGTC	2 cut(s)	1112, 1304
ScrFI	CCNGG	2 cut(s)	320, 641
SduI	GDGCHC	3 cut(s)	196, 244, 1076
SfaNI	GCATC	6 cut(s)	28, 48, 108, 342, 964, 1120
SfcI	CTRYAG	2 cut(s)	8, 46
SinI	GGWCC	2 cut(s)	600, 655
SmiMI	CAYNNNNRTG	1 cut(s)	234
SmlI	CTYRAG	4 cut(s)	272, 428, 500, 539
SmoI	CTYRAG	4 cut(s)	272, 428, 500, 539
Sse9I	AATT	2 cut(s)	129, 513
SseBI	AGGCCT	1 cut(s)	140
SsiI	CCGC	1 cut(s)	551
SspMI	CTAG	1 cut(s)	633
SstI	GAGCTC	1 cut(s)	244
StuI	AGGCCT	1 cut(s)	140
StyD4I	CCNGG	2 cut(s)	318, 639
StyI	CCWWGG	4 cut(s)	62, 141, 865, 1020
TaaI	ACNGT	1 cut(s)	32
TaiI	ACGT	2 cut(s)	114, 227
TaqI	TCGA	1 cut(s)	729
TasI	AATT	2 cut(s)	129, 513
TatI	WGTACW	1 cut(s)	1044
TauI	GCSGC	1 cut(s)	554
TfiI	GAWTC	3 cut(s)	358, 433, 726
Tru1I	TTAA	9 cut(s)	117, 234, 285, 363, 429, 540, 771, 1223, 1263
Tru9I	TTAA	9 cut(s)	117, 234, 285, 363, 429, 540, 771, 1223, 1263
TscAI	CASTG	5 cut(s)	256, 305, 692, 784, 919
TseFI	GTSAC	1 cut(s)	32
TseI	GCWGC	4 cut(s)	371, 496, 662, 713
Tsp45I	GTSAC	1 cut(s)	32
TspDTI	ATGAA	5 cut(s)	271, 630, 1188, 1217, 1261
TspRI	CASTG	5 cut(s)	256, 305, 692, 784, 919
Vha464I	CTTAAG	2 cut(s)	428, 539
VpaK11BI	GGWCC	2 cut(s)	600, 655
XagI	CCTNNNNNAGG	1 cut(s)	46
XmiI	GTMKAC	1 cut(s)	161
XspI	CTAG	1 cut(s)	633

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.