Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009418

Physical Location & Seq

Genomic Coordinates Forward (+)

33253304 .. 33256565

Gene Structure 3262 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1259500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGCTTTACTGAACATTGGTGCTGGGAACAGCGACGATGCCTTCTACAGGTATAAGATGCCCAGGATGGTTACCAAAATTGAGGGCCGTGGAAATGGCATCAAGACAAATATAGTCAACATGGTGGACATCGCAAAGGCCTTGGCAAGGCCCCCTGCATATACTACAAAGTATTTTGGTTGTGAACTTGGAGCCCAATCCAAATTTGATGAGAAAACTGGGACTTCTATTGTTAATGGGTCCCATGACACCGCTAAGCTAGCTGGCCTTCTTGAAAATTTTATTAAGAAATACGTCCAGTGTTATGGATGTGGCAATCCAGAAACAGAGATCTTGATTACGAAGACTCAAATGATTCAACTGAAATGTGCTGCGTGTGGTTTTGTGTCTGATGTCGACATGAGAGACAAGCTCACTACCTTCATTATTAAGAACCCACCCGAGCAGAAAAAGGGATCCAAGGACAAGAAAGCAATGAGGAGGGCTGAAAAGGAACGGTTGAAAGAAGGTGAAGCTGCTGATGAGGAGCTGAAGAAACAAAAGAAAGAGGCAAAGAAGAAGGGCACTTCTTCCTCTTCTAAGGAGGGCCCTGTGAAAGCCAGCTCTTCGAAAAAAAAAACAAGTGGATCTGATGAGGACCGCACATCACCTACTCACAGCCAGGCCGATGAGGAGGAGGCCAATGATGTTGATGATGATGATGATGATGTTCAGTGGCAAACTGATACATCACTAGAGGCAGCTCGTCAACGGATTCAAGAACAATTGAGTGCTGCGACAGCTGATATGGTAATGCTTTCAACCAATGAACCAGAGAAGCCCAAGGAAGCAACAAAGGCTGTGGAGAATGGAAACTCCACTGCTCGAGTAACACTTACTGATGAGTTGAAAAAGAATCTAGATAAAGGTATCTCAGCCAAACAGTTACAATCCCTTCTGCCGTCACTTTCAGGGTCTGCTAAGGAAAAGATGACTGCTCTTTTTGAAGCGCTATTTGGGGGCATAGAGAAAGGGTTTGCAAAGGAGGTGTCGAAGAAGATAAAGTACCTTGCTGTAGCTGCTGCACAGGATGAGGGATCCCAGTTGCTCGTGCTTCAAGCAATTGAAGGCTTCTGTGGGAAGTCAAGCTCAAGTGCATTGAAGGAGGTTGCCCTGGTTTTAAAAGCTCTCTACGATGCTGATATCTTGGAAGAAGAAACTATAGTGCAGTGGTATCAAGGTGGACTGAAGGGAGCCGGCAGGGGCTCTCAGATTTGGAAGAACACTAAACCCTTCATTGACTGGCTCCAGAGTGCTGAGTCTGAAACTGAGGAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03013 ko04214 map03013 map04214

Pfam Domains

Protein Families

W2 eIF-5_eIF-2B

Protein Analysis

440

Amino Acids

48.01

Weight (kDa)

5.88

Isoelectric Point (pI)

34.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	12 - 127	2.1e-35	Domain found in IF2B/IF5
W2	PF02020	361 - 439	9.6e-22	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36730 AT1G77840
fragaria_vesca	FvH4_5g38780
malus_domestica	MD00G1104000.v1.1 MD09G1259500.v1.1 MD15G1359300.v1.1
prunus_persica	Prupe.1G506900_v2.0.a1 Prupe.3G151100_v2.0.a1
pyrus_communis	pycom08g14860 pycom09g17420 pycom15g32170
rosa_chinensis	RchiOBHm_Chr7g0240701
rosa_laevigata	RLG00000000625
rosa_multiflora	Rmu_sc0004799.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219650
rosa_rugosa	Rorug07G0338400 Rorug07G0338400
rosa_samantha	Rh7AG493000 Rh7BG465000 Rh7CG511400 Rh7DG478200
rosa_wichuraiana	Rw7G041700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	396
AciI	CCGC	2 cut(s)	252, 640
AclWI	GGATC	5 cut(s)	450, 463, 634, 1071, 1084
AcsI	RAATTY	2 cut(s)	203, 277
AcuI	CTGAAG	2 cut(s)	551, 1247
AfaI	GTAC	1 cut(s)	1046
AfeI	AGCGCT	1 cut(s)	990
AfiI	CCNNNNNNNGG	2 cut(s)	48, 147
AjnI	CCWGG	3 cut(s)	62, 660, 1152
AjuI	GAANNNNNNNTTGG	2 cut(s)	978, 1010
Alw26I	GTCTC	1 cut(s)	399
AlwI	GGATC	5 cut(s)	450, 463, 634, 1071, 1084
AlwNI	CAGNNNCTG	1 cut(s)	956
Ama87I	CYCGRG	2 cut(s)	440, 864
Aor51HI	AGCGCT	1 cut(s)	990
AoxI	GGCC	7 cut(s)	85, 138, 149, 265, 586, 663, 678
ApaI	GGGCCC	1 cut(s)	590
ApeKI	GCWGC	6 cut(s)	371, 515, 740, 773, 1058, 1061
ApoI	RAATTY	2 cut(s)	203, 277
ArsI	GACNNNNNNTTYG	2 cut(s)	1013, 1045
AspLEI	GCGC	1 cut(s)	991
AspS9I	GGNCC	6 cut(s)	85, 150, 240, 586, 587, 637
AsuHPI	GGTGA	2 cut(s)	521, 639
AsuII	TTCGAA	1 cut(s)	608
AsuNHI	GCTAGC	1 cut(s)	259
AvaI	CYCGRG	2 cut(s)	440, 864
AvaII	GGWCC	2 cut(s)	240, 637
BaeGI	GKGCMC	2 cut(s)	566, 590
BamHI	GGATCC	2 cut(s)	455, 1076
BanII	GRGCYC	3 cut(s)	196, 590, 1247
BarI	GAAGNNNNNNTAC	2 cut(s)	1028, 1060
BauI	CACGAG	1 cut(s)	1088
BbsI	GAAGAC	1 cut(s)	350
BbvI	GCAGC	6 cut(s)	358, 502, 752, 760, 1045, 1048
BccI	CCATC	1 cut(s)	61
BceAI	ACGGC	2 cut(s)	72, 925
BciT130I	CCWGG	3 cut(s)	64, 662, 1154
BcoDI	GTCTC	1 cut(s)	399
BfaI	CTAG	3 cut(s)	260, 734, 899
BfmI	CTRYAG	3 cut(s)	46, 1053, 1200
BfoI	RGCGCY	1 cut(s)	992
BglII	AGATCT	1 cut(s)	330
BisI	GCNGC	6 cut(s)	372, 516, 741, 774, 1059, 1062
BlpI	GCTNAGC	1 cut(s)	255
BlsI	GCNGC	6 cut(s)	373, 517, 742, 775, 1060, 1063
Bme1390I	CCNGG	3 cut(s)	64, 662, 1154
Bme18I	GGWCC	2 cut(s)	240, 637
BmeT110I	CYCGRG	2 cut(s)	440, 864
BmgT120I	GGNCC	6 cut(s)	85, 150, 240, 586, 587, 637
BmiI	GGNNCC	9 cut(s)	152, 193, 241, 242, 457, 588, 1078, 1234, 1286
BmrFI	CCNGG	3 cut(s)	64, 662, 1154
BmrI	ACTGGG	2 cut(s)	228, 1075
BmsI	GCATC	4 cut(s)	28, 48, 108, 1165
BmtI	GCTAGC	1 cut(s)	263
BmuI	ACTGGG	2 cut(s)	228, 1075
BpiI	GAAGAC	1 cut(s)	350
BplI	GAGNNNNNCTC	2 cut(s)	396, 428
BpmI	CTGGAG	1 cut(s)	1271
Bpu10I	CCTNAGC	1 cut(s)	960
Bpu1102I	GCTNAGC	1 cut(s)	255
Bpu14I	TTCGAA	1 cut(s)	608
BpuEI	CTTGAG	1 cut(s)	1114
BsaJI	CCNNGG	6 cut(s)	62, 88, 141, 459, 822, 1152
Bsc4I	CCNNNNNNNGG	2 cut(s)	48, 147
Bse118I	RCCGGY	1 cut(s)	1235
Bse1I	ACTGG	4 cut(s)	223, 298, 1081, 1286
Bse3DI	GCAATG	1 cut(s)	480
BseBI	CCWGG	3 cut(s)	64, 662, 1154
BseDI	CCNNGG	6 cut(s)	62, 88, 141, 459, 822, 1152
BseGI	GGATG	3 cut(s)	72, 314, 1075
BseLI	CCNNNNNNNGG	2 cut(s)	48, 147
BseMI	GCAATG	1 cut(s)	480
BseMII	CTCAG	4 cut(s)	927, 1262, 1287, 1299
BseNI	ACTGG	4 cut(s)	223, 298, 1081, 1286
BseRI	GAGGAG	4 cut(s)	493, 539, 686, 689
BseSI	GKGCMC	2 cut(s)	566, 590
BseXI	GCAGC	6 cut(s)	358, 502, 752, 760, 1045, 1048
BseYI	CCCAGC	1 cut(s)	23
BsgI	GTGCAG	2 cut(s)	1047, 1226
BshFI	GGCC	7 cut(s)	87, 140, 151, 267, 588, 665, 680
BsiHKCI	CYCGRG	2 cut(s)	440, 864
BsiSI	CCGG	1 cut(s)	1236
BslFI	GGGAC	2 cut(s)	226, 235
BslI	CCNNNNNNNGG	2 cut(s)	48, 147
BsmAI	GTCTC	1 cut(s)	399
BsmFI	GGGAC	2 cut(s)	226, 235
BsnI	GGCC	7 cut(s)	87, 140, 151, 267, 588, 665, 680
BsoBI	CYCGRG	2 cut(s)	440, 864
Bsp119I	TTCGAA	1 cut(s)	608
Bsp120I	GGGCCC	1 cut(s)	586
Bsp1286I	GDGCHC	4 cut(s)	196, 566, 590, 1247
Bsp143I	GATC	4 cut(s)	330, 455, 626, 1076
Bsp1720I	GCTNAGC	1 cut(s)	255
BspACI	CCGC	2 cut(s)	252, 640
BspANI	GGCC	7 cut(s)	87, 140, 151, 267, 588, 665, 680
BspCNI	CTCAG	4 cut(s)	926, 1261, 1288, 1300
BspLI	GGNNCC	9 cut(s)	152, 193, 241, 242, 457, 588, 1078, 1234, 1286
BspOI	GCTAGC	1 cut(s)	263
BspPI	GGATC	5 cut(s)	450, 463, 634, 1071, 1084
BspQI	GCTCTTC	1 cut(s)	610
BspT104I	TTCGAA	1 cut(s)	608
BsrDI	GCAATG	1 cut(s)	480
BsrFI	RCCGGY	1 cut(s)	1235
BsrI	ACTGG	4 cut(s)	223, 298, 1081, 1286
BssAI	RCCGGY	1 cut(s)	1235
BssECI	CCNNGG	6 cut(s)	62, 88, 141, 459, 822, 1152
BssMI	GATC	4 cut(s)	330, 455, 626, 1076
BssSI	CACGAG	1 cut(s)	1088
BssT1I	CCWWGG	3 cut(s)	141, 459, 822
Bst2BI	CACGAG	1 cut(s)	1088
Bst2UI	CCWGG	3 cut(s)	64, 662, 1154
Bst4CI	ACNGT	2 cut(s)	498, 924
Bst6I	CTCTTC	2 cut(s)	580, 610
BstBI	TTCGAA	1 cut(s)	608
BstC8I	GCNNGC	4 cut(s)	261, 265, 601, 1237
BstDEI	CTNAG	7 cut(s)	255, 579, 913, 960, 1248, 1296, 1308
BstDSI	CCRYGG	1 cut(s)	88
BstEII	GGTNACC	1 cut(s)	70
BstENI	CCTNNNNNAGG	2 cut(s)	46, 145
BstF5I	GGATG	3 cut(s)	72, 314, 1075
BstH2I	RGCGCY	1 cut(s)	992
BstHHI	GCGC	1 cut(s)	991
BstKTI	GATC	4 cut(s)	333, 458, 629, 1079
BstMAI	GTCTC	1 cut(s)	399
BstMBI	GATC	4 cut(s)	330, 455, 626, 1076
BstMWI	GCNNNNNNNGC	4 cut(s)	260, 779, 836, 1058
BstNI	CCWGG	3 cut(s)	64, 662, 1154
BstPI	GGTNACC	1 cut(s)	70
BstSCI	CCNGG	3 cut(s)	62, 660, 1152
BstSFI	CTRYAG	3 cut(s)	46, 1053, 1200
BstSLI	GKGCMC	2 cut(s)	566, 590
BstV1I	GCAGC	6 cut(s)	358, 502, 752, 760, 1045, 1048
BstV2I	GAAGAC	1 cut(s)	350
BstX2I	RGATCY	4 cut(s)	330, 455, 626, 1076
BstXI	CCANNNNNNTGG	1 cut(s)	305
BstYI	RGATCY	4 cut(s)	330, 455, 626, 1076
BsuRI	GGCC	7 cut(s)	87, 140, 151, 267, 588, 665, 680
BtgI	CCRYGG	1 cut(s)	88
BtgZI	GCGATG	1 cut(s)	115
BtsCI	GGATG	3 cut(s)	72, 314, 1075
BtsI	GCAGTG	2 cut(s)	858, 1214
BtsIMutI	CAGTG	4 cut(s)	305, 719, 858, 1214
Cac8I	GCNNGC	4 cut(s)	261, 265, 601, 1237
CaiI	CAGNNNCTG	1 cut(s)	956
CfoI	GCGC	1 cut(s)	991
Cfr10I	RCCGGY	1 cut(s)	1235
Cfr13I	GGNCC	6 cut(s)	85, 150, 240, 586, 587, 637
Csp6I	GTAC	1 cut(s)	1045
CspCI	CAANNNNNGTGG	2 cut(s)	822, 857
CviAII	CATG	3 cut(s)	121, 245, 400
CviQI	GTAC	1 cut(s)	1045
DdeI	CTNAG	7 cut(s)	255, 579, 913, 960, 1248, 1296, 1308
DpnI	GATC	4 cut(s)	332, 457, 628, 1078
DpnII	GATC	4 cut(s)	330, 455, 626, 1076
DraI	TTTAAA	1 cut(s)	1161
Eam1104I	CTCTTC	2 cut(s)	580, 610
EarI	CTCTTC	2 cut(s)	580, 610
Eco130I	CCWWGG	3 cut(s)	141, 459, 822
Eco147I	AGGCCT	1 cut(s)	140
Eco24I	GRGCYC	3 cut(s)	196, 590, 1247
Eco32I	GATATC	1 cut(s)	1183
Eco47I	GGWCC	2 cut(s)	240, 637
Eco47III	AGCGCT	1 cut(s)	990
Eco57I	CTGAAG	2 cut(s)	551, 1247
Eco88I	CYCGRG	2 cut(s)	440, 864
Eco91I	GGTNACC	1 cut(s)	70
EcoNI	CCTNNNNNAGG	2 cut(s)	46, 145
EcoO109I	RGGNCCY	4 cut(s)	150, 240, 586, 587
EcoO65I	GGTNACC	1 cut(s)	70
EcoRII	CCWGG	3 cut(s)	62, 660, 1152
EcoRV	GATATC	1 cut(s)	1183
EcoT14I	CCWWGG	3 cut(s)	141, 459, 822
EcoT38I	GRGCYC	3 cut(s)	196, 590, 1247
ErhI	CCWWGG	3 cut(s)	141, 459, 822
FaeI	CATG	3 cut(s)	124, 248, 403
FaqI	GGGAC	2 cut(s)	226, 235
FatI	CATG	3 cut(s)	120, 244, 399
FblI	GTMKAC	1 cut(s)	396
Fnu4HI	GCNGC	6 cut(s)	372, 516, 741, 774, 1059, 1062
FokI	GGATG	3 cut(s)	79, 321, 1082
FriOI	GRGCYC	3 cut(s)	196, 590, 1247
Fsp4HI	GCNGC	6 cut(s)	372, 516, 741, 774, 1059, 1062
FspBI	CTAG	3 cut(s)	260, 734, 899
GlaI	GCGC	1 cut(s)	990
GluI	GCNGC	6 cut(s)	372, 516, 741, 774, 1059, 1062
GsaI	CCCAGC	1 cut(s)	27
GsuI	CTGGAG	1 cut(s)	1271
HaeII	RGCGCY	1 cut(s)	992
HaeIII	GGCC	7 cut(s)	87, 140, 151, 267, 588, 665, 680
HapII	CCGG	1 cut(s)	1236
HhaI	GCGC	1 cut(s)	991
Hin1II	CATG	3 cut(s)	124, 248, 403
Hin6I	GCGC	1 cut(s)	989
HinP1I	GCGC	1 cut(s)	989
HincII	GTYRAC	3 cut(s)	118, 397, 749
HindII	GTYRAC	3 cut(s)	118, 397, 749
HinfI	GANTC	5 cut(s)	346, 355, 754, 895, 1298
HpaII	CCGG	1 cut(s)	1236
HphI	GGTGA	2 cut(s)	521, 639
Hpy166II	GTNNAC	6 cut(s)	118, 127, 185, 397, 749, 1223
Hpy188I	TCNGA	4 cut(s)	391, 631, 1251, 1303
Hpy188III	TCNNGA	7 cut(s)	103, 272, 320, 334, 758, 899, 1288
Hpy8I	GTNNAC	6 cut(s)	118, 127, 185, 397, 749, 1223
Hpy99I	CGWCG	1 cut(s)	38
HpyCH4III	ACNGT	2 cut(s)	498, 924
HpyCH4IV	ACGT	1 cut(s)	294
HpyCH4V	TGCA	5 cut(s)	158, 1019, 1064, 1136, 1207
HpyF10VI	GCNNNNNNNGC	4 cut(s)	260, 779, 836, 1058
HpyF3I	CTNAG	7 cut(s)	255, 579, 913, 960, 1248, 1296, 1308
HpySE526I	ACGT	1 cut(s)	294
Hsp92II	CATG	3 cut(s)	124, 248, 403
HspAI	GCGC	1 cut(s)	989
KflI	GGGWCCC	1 cut(s)	240
KroI	GCCGGC	1 cut(s)	1235
KroNI	GCCGGC	1 cut(s)	1237
Kzo9I	GATC	4 cut(s)	330, 455, 626, 1076
LguI	GCTCTTC	1 cut(s)	610
LmnI	GCTCC	4 cut(s)	191, 526, 1232, 1290
Lsp1109I	GCAGC	6 cut(s)	358, 502, 752, 760, 1045, 1048
LweI	GCATC	4 cut(s)	28, 48, 108, 1165
MaeI	CTAG	3 cut(s)	260, 734, 899
MaeII	ACGT	1 cut(s)	294
MaeIII	GTNAC	4 cut(s)	70, 868, 924, 942
MalI	GATC	4 cut(s)	332, 457, 628, 1078
MboI	GATC	4 cut(s)	330, 455, 626, 1076
MfeI	CAATTG	2 cut(s)	764, 1101
MflI	RGATCY	4 cut(s)	330, 455, 626, 1076
MhlI	GDGCHC	4 cut(s)	196, 566, 590, 1247
MluCI	AATT	5 cut(s)	78, 203, 277, 764, 1101
MlyI	GAGTC	2 cut(s)	340, 1307
MroNI	GCCGGC	1 cut(s)	1235
MseI	TTAA	4 cut(s)	234, 285, 429, 1160
MspA1I	CMGCKG	1 cut(s)	782
MspI	CCGG	1 cut(s)	1236
MspR9I	CCNGG	3 cut(s)	64, 662, 1154
MunI	CAATTG	2 cut(s)	764, 1101
MvaI	CCWGG	3 cut(s)	64, 662, 1154
MwoI	GCNNNNNNNGC	4 cut(s)	260, 779, 836, 1058
NaeI	GCCGGC	1 cut(s)	1237
NdeII	GATC	4 cut(s)	330, 455, 626, 1076
NgoMIV	GCCGGC	1 cut(s)	1235
NheI	GCTAGC	1 cut(s)	259
NlaIII	CATG	3 cut(s)	124, 248, 403
NlaIV	GGNNCC	9 cut(s)	152, 193, 241, 242, 457, 588, 1078, 1234, 1286
NmuCI	GTSAC	1 cut(s)	942
NspV	TTCGAA	1 cut(s)	608
PaeR7I	CTCGAG	1 cut(s)	864
PceI	AGGCCT	1 cut(s)	140
PciSI	GCTCTTC	1 cut(s)	610
PdiI	GCCGGC	1 cut(s)	1237
PfeI	GAWTC	3 cut(s)	355, 754, 895
PkrI	GCNGC	6 cut(s)	373, 517, 742, 775, 1060, 1063
PleI	GAGTC	2 cut(s)	340, 1306
PpsI	GAGTC	2 cut(s)	340, 1306
PpuMI	RGGWCCY	1 cut(s)	240
Psp5II	RGGWCCY	1 cut(s)	240
Psp6I	CCWGG	3 cut(s)	62, 660, 1152
PspEI	GGTNACC	1 cut(s)	70
PspFI	CCCAGC	1 cut(s)	23
PspGI	CCWGG	3 cut(s)	62, 660, 1152
PspN4I	GGNNCC	9 cut(s)	152, 193, 241, 242, 457, 588, 1078, 1234, 1286
PspOMI	GGGCCC	1 cut(s)	586
PspPI	GGNCC	6 cut(s)	85, 150, 240, 586, 587, 637
PspPPI	RGGWCCY	1 cut(s)	240
PspXI	VCTCGAGB	1 cut(s)	864
PstNI	CAGNNNCTG	1 cut(s)	956
PsuI	RGATCY	4 cut(s)	330, 455, 626, 1076
PvuII	CAGCTG	1 cut(s)	782
RsaI	GTAC	1 cut(s)	1046
RsaNI	GTAC	1 cut(s)	1045
SalI	GTCGAC	1 cut(s)	395
SapI	GCTCTTC	1 cut(s)	610
SaqAI	TTAA	4 cut(s)	234, 285, 429, 1160
SatI	GCNGC	6 cut(s)	372, 516, 741, 774, 1059, 1062
Sau3AI	GATC	4 cut(s)	330, 455, 626, 1076
Sau96I	GGNCC	6 cut(s)	85, 150, 240, 586, 587, 637
SchI	GAGTC	2 cut(s)	340, 1307
ScrFI	CCNGG	3 cut(s)	64, 662, 1154
SduI	GDGCHC	4 cut(s)	196, 566, 590, 1247
SfaNI	GCATC	4 cut(s)	28, 48, 108, 1165
SfcI	CTRYAG	3 cut(s)	46, 1053, 1200
Sfr274I	CTCGAG	1 cut(s)	864
SfuI	TTCGAA	1 cut(s)	608
SinI	GGWCC	2 cut(s)	240, 637
SlaI	CTCGAG	1 cut(s)	864
SmlI	CTYRAG	2 cut(s)	864, 1129
SmoI	CTYRAG	2 cut(s)	864, 1129
Sse9I	AATT	5 cut(s)	78, 203, 277, 764, 1101
SseBI	AGGCCT	1 cut(s)	140
SsiI	CCGC	2 cut(s)	252, 640
SspMI	CTAG	3 cut(s)	260, 734, 899
StuI	AGGCCT	1 cut(s)	140
StyD4I	CCNGG	3 cut(s)	62, 660, 1152
StyI	CCWWGG	3 cut(s)	141, 459, 822
TaaI	ACNGT	2 cut(s)	498, 924
TaiI	ACGT	1 cut(s)	297
TaqI	TCGA	4 cut(s)	396, 608, 865, 1031
TasI	AATT	5 cut(s)	78, 203, 277, 764, 1101
TfiI	GAWTC	3 cut(s)	355, 754, 895
Tru1I	TTAA	4 cut(s)	234, 285, 429, 1160
Tru9I	TTAA	4 cut(s)	234, 285, 429, 1160
TscAI	CASTG	4 cut(s)	305, 719, 865, 1214
TseFI	GTSAC	1 cut(s)	942
TseI	GCWGC	6 cut(s)	371, 515, 740, 773, 1058, 1061
Tsp45I	GTSAC	1 cut(s)	942
TspDTI	ATGAA	3 cut(s)	412, 822, 1264
TspGWI	ACGGA	1 cut(s)	766
TspRI	CASTG	4 cut(s)	305, 719, 865, 1214
VpaK11BI	GGWCC	2 cut(s)	240, 637
XagI	CCTNNNNNAGG	2 cut(s)	46, 145
XapI	RAATTY	2 cut(s)	203, 277
XbaI	TCTAGA	1 cut(s)	898
XhoI	CTCGAG	1 cut(s)	864
XmiI	GTMKAC	1 cut(s)	396
XspI	CTAG	3 cut(s)	260, 734, 899

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.