Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009418

Physical Location & Seq

Genomic Coordinates Reverse (-)

21884352 .. 21887417

Gene Structure 3066 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1104000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGCTCTACAAAACATCGGTGCTTCAAACCGTGATGATGCCTTCTACAGGTATAAGATGCCAAGGATGATCACTAAGATTGAAGGCAGAGGAAATGGCATCAAGACTAATGTGGTTAACATGGTTGATATTGCAAAGGCCTTGGCTAGACCTGCTTCCTACACTACAAAGTATTTTGGCTGTGAGCTTGGAGCCCAATCAAAATTTGATGAGAAGACTGGAACTTCCCACGTGAATGGTGCTCATGACACTGCTAAGCTTGCTGGACTTCTTGAGAACTTCATCAAGAAATATGTGCAGTGCTATGGTTGCGGGAACCCAGAAACCGAAATTATTATAACCAAGACGCAGATGCTAAATCTGAAATGTGCTGCATGTGGGTTTGTGTCTGATGTTGATATGAGGGACAAGCTCACAACTTTTATCCTTAAGAATCCTCCAGAACAGAAGAAGGCGGTTAAAGGAAAGAAAGGCTTGAGGAGGGCCGACAAAGAACAACTTAAGGAAGGGGAGGCCGCTGACGAGGAGCAAAAGAAGCTTAAGAAGGAGGCAGCAAAGAAGAAAGGAACTTCGGGTTCGAGAGCATCAAGCAAGAAGAAAAGCAATGGTTCTGATGAGGATCGCTCCCCTGCTCACAGCCAGGGAGATGAGAACGACCAAGCTGCTGAGGAGGATGGGGATGATGATGTCCAGTGGCAGACGGACACTTCTGTCGAGGCTGCTAAGAAGCGAATCCAGGAGCAGTTGAGTGCTGTTACTGCTGATATGGTGATGCTTTCTACCATTGAAGAGAAAAAGTCACCAAAGCAGTCTCCTGATCGTGAAGTGAAGAAGCCTGAGACAAAGAGCCATCAGAACGGTGCTGATTGTTATACCCACGAAAGACTTGTTAATGAGATCAAACAATCCTTGCAAAAGGGGACGTCTGCAGCTCAGCTTAAATCCTTTTTGGGCACTCTTTCTGGCACACCCCAAGAAGTTATGGATGCCCTACTCGAGGCTCTCTTTGAAGGTGTGGGAAAGGGGTTCTCTAAGGAGGTTTCTAAGAAGAAAAACTACCTGGCTGCAGCAACCCGGATGGAGGGATCTCAGACAGTTCTATTGCAAGCTATCGAGTCTTTCAGTGGGAAGTCTAACCCTGATGCTGCAAAGGAAGTGGCTCTTGTTCTAAAACTGTTGTACGATGTTGACATTCTGGAGGAGGAATACATTATGAAGTGGTACCAACGGGGCATAGCTGGGAAGAACAAGAGCTCCCCGATTTGGAAGAATGCCAAGCCGTTCATCGGGTGGCTCCAGAGCGCCGAGTCTGAAACGGAGGAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03013 ko04214 map03013 map04214

Pfam Domains

Protein Families

W2 eIF-5_eIF-2B

Protein Analysis

443

Amino Acids

48.8

Weight (kDa)

8.44

Isoelectric Point (pI)

37.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
eIF-5_eIF-2B	PF01873	11 - 127	1.9e-34	Domain found in IF2B/IF5
W2	PF02020	364 - 442	2.7e-19	eIF4-gamma/eIF5/eIF2-epsilon

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009418)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36730 AT1G77840
fragaria_vesca	FvH4_5g38780
malus_domestica	MD00G1104000.v1.1 MD09G1259500.v1.1 MD15G1359300.v1.1
prunus_persica	Prupe.1G506900_v2.0.a1 Prupe.3G151100_v2.0.a1
pyrus_communis	pycom08g14860 pycom09g17420 pycom15g32170
rosa_chinensis	RchiOBHm_Chr7g0240701
rosa_laevigata	RLG00000000625
rosa_multiflora	Rmu_sc0004799.1_g000002
rosa_roxburghii	Rroxscaffold_3G00219650
rosa_rugosa	Rorug07G0338400 Rorug07G0338400
rosa_samantha	Rh7AG493000 Rh7BG465000 Rh7CG511400 Rh7DG478200
rosa_wichuraiana	Rw7G041700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	338
AasI	GACNNNNNNGTC	1 cut(s)	710
AatII	GACGTC	1 cut(s)	926
Acc36I	ACCTGC	1 cut(s)	160
Acc65I	GGTACC	1 cut(s)	1221
AccB1I	GGYRCC	1 cut(s)	1221
AciI	CCGC	3 cut(s)	312, 455, 516
AclWI	GGATC	2 cut(s)	627, 1093
AcsI	RAATTY	1 cut(s)	203
AcvI	CACGTG	1 cut(s)	232
AcyI	GRCGYC	1 cut(s)	923
AfaI	GTAC	2 cut(s)	1181, 1223
AfiI	CCNNNNNNNGG	5 cut(s)	48, 997, 1081, 1263, 1286
AflII	CTTAAG	3 cut(s)	428, 500, 539
AgsI	TTSAA	4 cut(s)	27, 83, 788, 1010
AjnI	CCWGG	3 cut(s)	639, 735, 1059
AloI	GAACNNNNNNTCC	2 cut(s)	1238, 1270
Alw21I	GWGCWC	2 cut(s)	244, 1256
Alw26I	GTCTC	2 cut(s)	816, 833
AlwI	GGATC	2 cut(s)	627, 1093
Ama87I	CYCGRG	1 cut(s)	995
AoxI	GGCC	3 cut(s)	138, 483, 513
ApeKI	GCWGC	8 cut(s)	371, 551, 662, 719, 929, 1064, 1067, 1145
ApoI	RAATTY	1 cut(s)	203
Asp718I	GGTACC	1 cut(s)	1221
AspLEI	GCGC	1 cut(s)	1304
AspS9I	GGNCC	1 cut(s)	483
AsuC2I	CCSGG	1 cut(s)	1075
AsuHPI	GGTGA	2 cut(s)	781, 792
AvaI	CYCGRG	1 cut(s)	995
BaeGI	GKGCMC	1 cut(s)	956
BanI	GGYRCC	1 cut(s)	1221
BanII	GRGCYC	2 cut(s)	196, 1256
BarI	GAAGNNNNNNTAC	2 cut(s)	1040, 1072
BbrPI	CACGTG	1 cut(s)	232
BbsI	GAAGAC	1 cut(s)	221
Bbv12I	GWGCWC	2 cut(s)	244, 1256
BbvCI	CCTCAGC	1 cut(s)	666
BbvI	GCAGC	8 cut(s)	358, 563, 649, 706, 941, 1051, 1079, 1132
BccI	CCATC	3 cut(s)	668, 858, 1072
BceAI	ACGGC	1 cut(s)	1264
BcgI	CGANNNNNNTGC	2 cut(s)	644, 678
BciT130I	CCWGG	3 cut(s)	641, 737, 1061
BclI	TGATCA	1 cut(s)	69
BcnI	CCSGG	1 cut(s)	1075
BcoDI	GTCTC	2 cut(s)	816, 833
BfaI	CTAG	1 cut(s)	147
BfmI	CTRYAG	3 cut(s)	46, 927, 1065
BfoI	RGCGCY	1 cut(s)	1305
BfrI	CTTAAG	3 cut(s)	428, 500, 539
BfuAI	ACCTGC	1 cut(s)	160
BisI	GCNGC	9 cut(s)	372, 516, 552, 663, 720, 930, 1065, 1068, 1146
BlpI	GCTNAGC	2 cut(s)	255, 933
BlsI	GCNGC	9 cut(s)	373, 517, 553, 664, 721, 931, 1066, 1069, 1147
Bme1390I	CCNGG	4 cut(s)	641, 737, 1061, 1075
BmeT110I	CYCGRG	1 cut(s)	995
BmgT120I	GGNCC	1 cut(s)	483
BmiI	GGNNCC	4 cut(s)	193, 317, 1223, 1295
BmrFI	CCNGG	4 cut(s)	641, 737, 1061, 1075
BmsI	GCATC	8 cut(s)	28, 48, 108, 342, 593, 762, 976, 1132
BpiI	GAAGAC	1 cut(s)	221
BplI	GAGNNNNNCTC	2 cut(s)	608, 640
BpmI	CTGGAG	3 cut(s)	423, 1217, 1280
Bpu10I	CCTNAGC	1 cut(s)	666
Bpu1102I	GCTNAGC	2 cut(s)	255, 933
BpuEI	CTTGAG	2 cut(s)	293, 496
BpuMI	CCSGG	1 cut(s)	1075
BsaAI	YACGTR	1 cut(s)	232
BsaBI	GATNNNNATC	1 cut(s)	618
BsaHI	GRCGYC	1 cut(s)	923
BsaJI	CCNNGG	3 cut(s)	62, 141, 640
BsaXI	ACNNNNNCTCC	3 cut(s)	1238, 1268, 1310
Bsc4I	CCNNNNNNNGG	5 cut(s)	48, 997, 1081, 1263, 1286
Bse1I	ACTGG	2 cut(s)	223, 691
Bse3DI	GCAATG	1 cut(s)	610
Bse8I	GATNNNNATC	1 cut(s)	618
BseBI	CCWGG	3 cut(s)	641, 737, 1061
BseDI	CCNNGG	3 cut(s)	62, 141, 640
BseGI	GGATG	5 cut(s)	72, 679, 685, 991, 1083
BseJI	GATNNNNATC	1 cut(s)	618
BseLI	CCNNNNNNNGG	5 cut(s)	48, 997, 1081, 1263, 1286
BseMI	GCAATG	1 cut(s)	610
BseMII	CTCAG	4 cut(s)	657, 828, 947, 1103
BseNI	ACTGG	2 cut(s)	223, 691
BseRI	GAGGAG	4 cut(s)	493, 539, 683, 1214
BseSI	GKGCMC	1 cut(s)	956
BseXI	GCAGC	8 cut(s)	358, 563, 649, 706, 941, 1051, 1079, 1132
BseYI	CCCAGC	1 cut(s)	1238
BsgI	GTGCAG	1 cut(s)	317
BshFI	GGCC	3 cut(s)	140, 485, 515
BshNI	GGYRCC	1 cut(s)	1221
BsiHKAI	GWGCWC	2 cut(s)	244, 1256
BsiHKCI	CYCGRG	1 cut(s)	995
BsiSI	CCGG	1 cut(s)	1075
BslFI	GGGAC	2 cut(s)	419, 934
BslI	CCNNNNNNNGG	5 cut(s)	48, 997, 1081, 1263, 1286
BsmAI	GTCTC	2 cut(s)	816, 833
BsmFI	GGGAC	2 cut(s)	419, 934
BsmI	GAATGC	1 cut(s)	1276
BsnI	GGCC	3 cut(s)	140, 485, 515
BsoBI	CYCGRG	1 cut(s)	995
Bsp1286I	GDGCHC	4 cut(s)	196, 244, 956, 1256
Bsp143I	GATC	5 cut(s)	69, 619, 817, 897, 1085
Bsp1720I	GCTNAGC	2 cut(s)	255, 933
BspACI	CCGC	3 cut(s)	312, 455, 516
BspANI	GGCC	3 cut(s)	140, 485, 515
BspCNI	CTCAG	4 cut(s)	658, 829, 946, 1102
BspHI	TCATGA	1 cut(s)	244
BspLI	GGNNCC	4 cut(s)	193, 317, 1223, 1295
BspMAI	CTGCAG	2 cut(s)	931, 1069
BspMI	ACCTGC	1 cut(s)	160
BspPI	GGATC	2 cut(s)	627, 1093
BspT107I	GGYRCC	1 cut(s)	1221
BspTI	CTTAAG	3 cut(s)	428, 500, 539
BsrDI	GCAATG	1 cut(s)	610
BsrI	ACTGG	2 cut(s)	223, 691
BssECI	CCNNGG	3 cut(s)	62, 141, 640
BssMI	GATC	5 cut(s)	69, 619, 817, 897, 1085
BssNI	GRCGYC	1 cut(s)	923
BssT1I	CCWWGG	2 cut(s)	62, 141
Bst2UI	CCWGG	3 cut(s)	641, 737, 1061
Bst4CI	ACNGT	4 cut(s)	32, 860, 1096, 1176
Bst6I	CTCTTC	2 cut(s)	783, 1317
BstACI	GRCGYC	1 cut(s)	923
BstAFI	CTTAAG	3 cut(s)	428, 500, 539
BstBAI	YACGTR	1 cut(s)	232
BstC8I	GCNNGC	2 cut(s)	261, 1107
BstDEI	CTNAG	9 cut(s)	75, 255, 666, 723, 837, 933, 1032, 1044, 1089
BstENI	CCTNNNNNAGG	2 cut(s)	46, 995
BstF5I	GGATG	5 cut(s)	72, 679, 685, 991, 1083
BstH2I	RGCGCY	1 cut(s)	1305
BstHHI	GCGC	1 cut(s)	1304
BstKTI	GATC	5 cut(s)	72, 622, 820, 900, 1088
BstMAI	GTCTC	2 cut(s)	816, 833
BstMBI	GATC	5 cut(s)	69, 619, 817, 897, 1085
BstMWI	GCNNNNNNNGC	5 cut(s)	152, 260, 309, 535, 758
BstNI	CCWGG	3 cut(s)	641, 737, 1061
BstNSI	RCATGY	1 cut(s)	378
BstSCI	CCNGG	4 cut(s)	639, 735, 1059, 1073
BstSFI	CTRYAG	3 cut(s)	46, 927, 1065
BstSLI	GKGCMC	1 cut(s)	956
BstV1I	GCAGC	8 cut(s)	358, 563, 649, 706, 941, 1051, 1079, 1132
BstV2I	GAAGAC	1 cut(s)	221
BstX2I	RGATCY	1 cut(s)	1085
BstXI	CCANNNNNNTGG	1 cut(s)	236
BstYI	RGATCY	1 cut(s)	1085
BsuRI	GGCC	3 cut(s)	140, 485, 515
BtsCI	GGATG	5 cut(s)	72, 679, 685, 991, 1083
BtsI	GCAGTG	2 cut(s)	249, 305
BtsIMutI	CAGTG	4 cut(s)	249, 305, 698, 1129
BveI	ACCTGC	1 cut(s)	160
Cac8I	GCNNGC	2 cut(s)	261, 1107
CciI	TCATGA	1 cut(s)	244
CfoI	GCGC	1 cut(s)	1304
Cfr13I	GGNCC	1 cut(s)	483
CseI	GACGC	1 cut(s)	355
Csp6I	GTAC	2 cut(s)	1180, 1222
CspCI	CAANNNNNGTGG	2 cut(s)	1137, 1172
CviAII	CATG	3 cut(s)	121, 245, 375
CviQI	GTAC	2 cut(s)	1180, 1222
DdeI	CTNAG	9 cut(s)	75, 255, 666, 723, 837, 933, 1032, 1044, 1089
DpnI	GATC	5 cut(s)	71, 621, 819, 899, 1087
DpnII	GATC	5 cut(s)	69, 619, 817, 897, 1085
DrdI	GACNNNNNNGTC	1 cut(s)	710
DseDI	GACNNNNNNGTC	1 cut(s)	710
Eam1104I	CTCTTC	2 cut(s)	783, 1317
EarI	CTCTTC	2 cut(s)	783, 1317
Ecl136II	GAGCTC	1 cut(s)	1254
Eco130I	CCWWGG	2 cut(s)	62, 141
Eco147I	AGGCCT	1 cut(s)	140
Eco24I	GRGCYC	2 cut(s)	196, 1256
Eco53kI	GAGCTC	1 cut(s)	1254
Eco72I	CACGTG	1 cut(s)	232
Eco88I	CYCGRG	1 cut(s)	995
EcoICRI	GAGCTC	1 cut(s)	1254
EcoNI	CCTNNNNNAGG	2 cut(s)	46, 995
EcoRII	CCWGG	3 cut(s)	639, 735, 1059
EcoT14I	CCWWGG	2 cut(s)	62, 141
EcoT38I	GRGCYC	2 cut(s)	196, 1256
ErhI	CCWWGG	2 cut(s)	62, 141
FaeI	CATG	3 cut(s)	124, 248, 378
FalI	AAGNNNNNCTT	6 cut(s)	458, 490, 483, 515, 1146, 1178
FaqI	GGGAC	2 cut(s)	419, 934
FatI	CATG	3 cut(s)	120, 244, 374
FauI	CCCGC	1 cut(s)	305
FbaI	TGATCA	1 cut(s)	69
Fnu4HI	GCNGC	9 cut(s)	372, 516, 552, 663, 720, 930, 1065, 1068, 1146
FokI	GGATG	5 cut(s)	79, 686, 692, 998, 1090
FriOI	GRGCYC	2 cut(s)	196, 1256
Fsp4HI	GCNGC	9 cut(s)	372, 516, 552, 663, 720, 930, 1065, 1068, 1146
FspBI	CTAG	1 cut(s)	147
GlaI	GCGC	1 cut(s)	1303
GluI	GCNGC	9 cut(s)	372, 516, 552, 663, 720, 930, 1065, 1068, 1146
GsaI	CCCAGC	1 cut(s)	1242
GsuI	CTGGAG	3 cut(s)	423, 1217, 1280
HaeII	RGCGCY	1 cut(s)	1305
HaeIII	GGCC	3 cut(s)	140, 485, 515
HapII	CCGG	1 cut(s)	1075
HgaI	GACGC	1 cut(s)	355
HhaI	GCGC	1 cut(s)	1304
Hin1I	GRCGYC	1 cut(s)	923
Hin1II	CATG	3 cut(s)	124, 248, 378
Hin6I	GCGC	1 cut(s)	1302
HinP1I	GCGC	1 cut(s)	1302
HincII	GTYRAC	2 cut(s)	118, 1189
HindII	GTYRAC	2 cut(s)	118, 1189
HindIII	AAGCTT	2 cut(s)	257, 536
HinfI	GANTC	4 cut(s)	433, 732, 1115, 1307
HpaI	GTTAAC	1 cut(s)	118
HpaII	CCGG	1 cut(s)	1075
HphI	GGTGA	2 cut(s)	781, 792
Hpy166II	GTNNAC	2 cut(s)	118, 1189
Hpy188I	TCNGA	6 cut(s)	363, 391, 613, 855, 1092, 1312
Hpy8I	GTNNAC	2 cut(s)	118, 1189
HpyAV	CCTTC	6 cut(s)	52, 77, 445, 500, 538, 1004
HpyCH4III	ACNGT	4 cut(s)	32, 860, 1096, 1176
HpyCH4IV	ACGT	2 cut(s)	231, 923
HpyCH4V	TGCA	8 cut(s)	134, 298, 374, 913, 929, 1067, 1105, 1148
HpyF10VI	GCNNNNNNNGC	5 cut(s)	152, 260, 309, 535, 758
HpyF3I	CTNAG	9 cut(s)	75, 255, 666, 723, 837, 933, 1032, 1044, 1089
HpySE526I	ACGT	2 cut(s)	231, 923
Hsp92I	GRCGYC	1 cut(s)	923
Hsp92II	CATG	3 cut(s)	124, 248, 378
HspAI	GCGC	1 cut(s)	1302
KpnI	GGTACC	1 cut(s)	1225
Ksp22I	TGATCA	1 cut(s)	69
KspAI	GTTAAC	1 cut(s)	118
Kzo9I	GATC	5 cut(s)	69, 619, 817, 897, 1085
LmnI	GCTCC	6 cut(s)	191, 526, 629, 739, 1259, 1299
Lsp1109I	GCAGC	8 cut(s)	358, 563, 649, 706, 941, 1051, 1079, 1132
LweI	GCATC	8 cut(s)	28, 48, 108, 342, 593, 762, 976, 1132
MaeI	CTAG	1 cut(s)	147
MaeII	ACGT	2 cut(s)	231, 923
MaeIII	GTNAC	2 cut(s)	754, 798
MalI	GATC	5 cut(s)	71, 621, 819, 899, 1087
MboI	GATC	5 cut(s)	69, 619, 817, 897, 1085
MboII	GAAGA	9 cut(s)	226, 460, 571, 607, 800, 841, 1060, 1255, 1279
MflI	RGATCY	1 cut(s)	1085
MhlI	GDGCHC	4 cut(s)	196, 244, 956, 1256
MluCI	AATT	2 cut(s)	203, 330
MlyI	GAGTC	2 cut(s)	1124, 1316
MseI	TTAA	7 cut(s)	117, 429, 459, 501, 540, 891, 939
MslI	CAYNNNNRTG	1 cut(s)	234
MspA1I	CMGCKG	1 cut(s)	518
MspCI	CTTAAG	3 cut(s)	428, 500, 539
MspI	CCGG	1 cut(s)	1075
MspR9I	CCNGG	4 cut(s)	641, 737, 1061, 1075
Mva1269I	GAATGC	1 cut(s)	1276
MvaI	CCWGG	3 cut(s)	641, 737, 1061
MwoI	GCNNNNNNNGC	5 cut(s)	152, 260, 309, 535, 758
NciI	CCSGG	1 cut(s)	1075
NdeII	GATC	5 cut(s)	69, 619, 817, 897, 1085
NlaIII	CATG	3 cut(s)	124, 248, 378
NlaIV	GGNNCC	4 cut(s)	193, 317, 1223, 1295
NmuCI	GTSAC	1 cut(s)	798
NspI	RCATGY	1 cut(s)	378
PaeR7I	CTCGAG	1 cut(s)	995
PagI	TCATGA	1 cut(s)	244
PceI	AGGCCT	1 cut(s)	140
PctI	GAATGC	1 cut(s)	1276
PfeI	GAWTC	2 cut(s)	433, 732
PfoI	TCCNGGA	1 cut(s)	735
PkrI	GCNGC	9 cut(s)	373, 517, 553, 664, 721, 931, 1066, 1069, 1147
PleI	GAGTC	2 cut(s)	1123, 1315
PmaCI	CACGTG	1 cut(s)	232
PmlI	CACGTG	1 cut(s)	232
PpsI	GAGTC	2 cut(s)	1123, 1315
Ppu21I	YACGTR	1 cut(s)	232
PsiI	TTATAA	1 cut(s)	338
Psp124BI	GAGCTC	1 cut(s)	1256
Psp6I	CCWGG	3 cut(s)	639, 735, 1059
PspCI	CACGTG	1 cut(s)	232
PspFI	CCCAGC	1 cut(s)	1238
PspGI	CCWGG	3 cut(s)	639, 735, 1059
PspN4I	GGNNCC	4 cut(s)	193, 317, 1223, 1295
PspPI	GGNCC	1 cut(s)	483
PspXI	VCTCGAGB	1 cut(s)	995
PstI	CTGCAG	2 cut(s)	931, 1069
PsuI	RGATCY	1 cut(s)	1085
RsaI	GTAC	2 cut(s)	1181, 1223
RsaNI	GTAC	2 cut(s)	1180, 1222
RseI	CAYNNNNRTG	1 cut(s)	234
SacI	GAGCTC	1 cut(s)	1256
SaqAI	TTAA	7 cut(s)	117, 429, 459, 501, 540, 891, 939
SatI	GCNGC	9 cut(s)	372, 516, 552, 663, 720, 930, 1065, 1068, 1146
Sau3AI	GATC	5 cut(s)	69, 619, 817, 897, 1085
Sau96I	GGNCC	1 cut(s)	483
SchI	GAGTC	2 cut(s)	1124, 1316
ScrFI	CCNGG	4 cut(s)	641, 737, 1061, 1075
SduI	GDGCHC	4 cut(s)	196, 244, 956, 1256
SfaNI	GCATC	8 cut(s)	28, 48, 108, 342, 593, 762, 976, 1132
SfcI	CTRYAG	3 cut(s)	46, 927, 1065
Sfr274I	CTCGAG	1 cut(s)	995
SlaI	CTCGAG	1 cut(s)	995
SmiMI	CAYNNNNRTG	1 cut(s)	234
SmlI	CTYRAG	6 cut(s)	272, 428, 475, 500, 539, 995
SmoI	CTYRAG	6 cut(s)	272, 428, 475, 500, 539, 995
Sse9I	AATT	2 cut(s)	203, 330
SseBI	AGGCCT	1 cut(s)	140
SsiI	CCGC	3 cut(s)	312, 455, 516
SspMI	CTAG	1 cut(s)	147
SstI	GAGCTC	1 cut(s)	1256
StuI	AGGCCT	1 cut(s)	140
StyD4I	CCNGG	4 cut(s)	639, 735, 1059, 1073
StyI	CCWWGG	2 cut(s)	62, 141
TaaI	ACNGT	4 cut(s)	32, 860, 1096, 1176
TaiI	ACGT	2 cut(s)	234, 926
TaqI	TCGA	4 cut(s)	578, 714, 996, 1113
TasI	AATT	2 cut(s)	203, 330
TauI	GCSGC	1 cut(s)	518
TfiI	GAWTC	2 cut(s)	433, 732
Tru1I	TTAA	7 cut(s)	117, 429, 459, 501, 540, 891, 939
Tru9I	TTAA	7 cut(s)	117, 429, 459, 501, 540, 891, 939
TscAI	CASTG	4 cut(s)	256, 305, 698, 1129
TseFI	GTSAC	1 cut(s)	798
TseI	GCWGC	8 cut(s)	371, 551, 662, 719, 929, 1064, 1067, 1145
Tsp45I	GTSAC	1 cut(s)	798
TspDTI	ATGAA	3 cut(s)	271, 1229, 1273
TspGWI	ACGGA	1 cut(s)	716
TspRI	CASTG	4 cut(s)	256, 305, 698, 1129
Vha464I	CTTAAG	3 cut(s)	428, 500, 539
XagI	CCTNNNNNAGG	2 cut(s)	46, 995
XapI	RAATTY	1 cut(s)	203
XceI	RCATGY	1 cut(s)	378
XhoI	CTCGAG	1 cut(s)	995
XspI	CTAG	1 cut(s)	147
ZraI	GACGTC	1 cut(s)	924

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.