AT1G80170

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009481
Physical Location & Seq
Reverse (-)
30153213 .. 30155894
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G80170.1

Sequence Viewer

Length: 1335 bp
ATGAGTTATTCACGCGGCGGTACTCTTGTCACTCTTTTGTTACTACTAGTCGTAGCCTCCTCACTCGCTTTGACCGCAAACGCGAATTCGTTCGAATCGCTCCTCCAGCTCCCGCGGAGACAGTCGCGCTCGACCCGACCCAGATCCGAACGGCTCCTCCACGTTGGCAATTTTGGTGCCAAAGGCAATGGCGTCACCGACGACACTAAAGCGTTTGCAGATGCTTGGAAGACAGCTTGTTCTTCCAAAGTCAAAACCAGAATCTTGGTGCCTGAAAATTACACTTGTCTCCTTCGGCCTATTGATCTTTCTGGACCTTGTAAAGCAAGACTCACACTCCAGATCTCTGGTACAATCATTGCTCCCAATGATCCAGATGTTTGGGAAGGTCTTAACCGCCGAAAATGGCTATATTTCCATGGTTTGAGCCGCTTAACTGTTGAAGGAGGCGGCACTGTTAACGGGATGGGGCAAGAATGGTGGGAAAGATCTTGCAAACACAATCATTCCAACCCTTGTCGAGGTGCTCCAACGGCCTTAACATTCCACAAGTGCAAGAATATGAGAGTTGAGAACCTCAATGTGATTGATAGTCAACAAATGCACATTGCCTTAACCAGCTGTCGCCGTGTGACCATCTCTGGTTTAAAGGTCATAGCTCCTGCTACTAGTCCCAATACTGATGGAATTCACATAAGCGTCTCTCGTGGTATCGTTATAGACAACACTACTGTCAGCACAGGAGATGACTGCATTTCGATAGTTAAAAACTCTACACAGATCTCCATCAGCAACATTATATGTGGTCCTGGCCATGGCATAAGCATTGGAAGCCTAGGAAAATCAAAATCTTGGGAAGAAGTTAGAGACATAACAGTTGATACGGCCATCATCTCTGACACTGCGAATGGTGTTCGGATCAAAACATGGCAGGGAGGGAGTGGTTTGGTCTCAAAAATTATCTTTAGGAACATCAAGATGAACAATGTGTCAAACCCCATCATCATCGATCAGTATTACTGCGATTCTAGGAAGCCGTGTGCGAATCAGACATCAGCTATTAGCATTGAGAACATCTCATTTGTTCACGTGAGGGGCACCTCAGCATCAAAGGAAGCAATCAAGATCTCTTGCAGCGATAGTTCACCATGTCGCAACATACTTCTACAAGACATTGATCTTGAACCATCAAACGGTGATGGTTTCACAGAGTCTTTCTGTTGGGAAGCTTATGGTTCTAGTTCGGGTCAAGTGTACCCTCCTCCTTGTCTTTCAGATGACACAAGCTTCTTAGAACAGTCGGTTCAGTCCGGGATCACATCTGCATACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

444

Amino Acids

48.17

Weight (kDa)

8.67

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 80 - 396 5.3e-99 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 176, 268, 1097
AccII CGCG 4 cut(s) 15, 83, 115, 127
AciI CCGC 8 cut(s) 15, 18, 75, 113, 115, 397, 430, 450
AclWI GGATC 4 cut(s) 138, 365, 926, 1322
AcoI YGGCCR 2 cut(s) 811, 885
AcsI RAATTY 2 cut(s) 85, 687
AcvI CACGTG 1 cut(s) 1090
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 3 cut(s) 22, 352, 1256
AfiI CCNNNNNNNGG 3 cut(s) 521, 815, 1193
AgsI TTSAA 2 cut(s) 443, 1184
AhlI ACTAGT 2 cut(s) 46, 668
AjnI CCWGG 1 cut(s) 808
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 7 cut(s) 109, 236, 621, 659, 1058, 1229, 1287
AluI AGCT 7 cut(s) 109, 236, 621, 659, 1058, 1229, 1287
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 5 cut(s) 112, 293, 706, 861, 955
AlwI GGATC 4 cut(s) 138, 365, 926, 1322
AoxI GGCC 4 cut(s) 296, 534, 811, 885
ApeKI GCWGC 1 cut(s) 1134
ApoI RAATTY 2 cut(s) 85, 687
Asp700I GAANNNNTTC 1 cut(s) 89
AspA2I CCTAGG 1 cut(s) 835
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 2 cut(s) 314, 806
AsuC2I CCSGG 1 cut(s) 1312
AsuHPI GGTGA 3 cut(s) 187, 1137, 1208
AsuII TTCGAA 1 cut(s) 93
AvaII GGWCC 2 cut(s) 314, 806
AvrII CCTAGG 1 cut(s) 835
BaeGI GKGCMC 1 cut(s) 1100
BaeI ACNNNNGTAYC 2 cut(s) 12, 45
BalI TGGCCA 1 cut(s) 813
BanI GGYRCC 3 cut(s) 176, 268, 1097
BauI CACGAG 1 cut(s) 705
BbrPI CACGTG 1 cut(s) 1090
BbsI GAAGAC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 529
BbvCI CCTCAGC 1 cut(s) 1102
BbvI GCAGC 1 cut(s) 1146
BccI CCATC 8 cut(s) 460, 644, 677, 794, 896, 1007, 1193, 1195
BceAI ACGGC 5 cut(s) 167, 549, 612, 900, 1021
BciT130I CCWGG 1 cut(s) 810
BcnI CCSGG 1 cut(s) 1312
BcoDI GTCTC 5 cut(s) 112, 293, 706, 861, 955
BcuI ACTAGT 2 cut(s) 46, 668
BfaI CTAG 5 cut(s) 47, 669, 836, 1029, 1239
BglII AGATCT 4 cut(s) 342, 488, 780, 1125
BisI GCNGC 4 cut(s) 16, 430, 451, 1135
BlnI CCTAGG 1 cut(s) 835
BlsI GCNGC 4 cut(s) 17, 431, 452, 1136
Bme1390I CCNGG 2 cut(s) 810, 1312
Bme18I GGWCC 2 cut(s) 314, 806
BmgT120I GGNCC 2 cut(s) 314, 806
BmiI GGNNCC 4 cut(s) 155, 178, 270, 1099
BmrFI CCNGG 2 cut(s) 810, 1312
BmsI GCATC 2 cut(s) 211, 1115
BpiI GAAGAC 1 cut(s) 236
BplI GAGNNNNNCTC 2 cut(s) 1061, 1093
BpmI CTGGAG 2 cut(s) 89, 323
Bpu10I CCTNAGC 1 cut(s) 1102
Bpu14I TTCGAA 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 1312
Bsa29I ATCGAT 1 cut(s) 1008
BsaAI YACGTR 1 cut(s) 1090
BsaBI GATNNNNATC 1 cut(s) 785
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 955
BsaJI CCNNGG 4 cut(s) 113, 418, 814, 835
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 321, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 521, 815, 1193
Bse3DI GCAATG 3 cut(s) 193, 357, 606
Bse8I GATNNNNATC 1 cut(s) 785
BseBI CCWGG 1 cut(s) 810
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 4 cut(s) 113, 418, 814, 835
BseGI GGATG 1 cut(s) 471
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 3 cut(s) 521, 815, 1193
BseMI GCAATG 3 cut(s) 193, 357, 606
BseMII CTCAG 1 cut(s) 1116
BseRI GAGGAG 4 cut(s) 49, 92, 146, 1251
BseSI GKGCMC 1 cut(s) 1100
BseXI GCAGC 1 cut(s) 1146
Bsh1236I CGCG 4 cut(s) 15, 83, 115, 127
BshFI GGCC 4 cut(s) 298, 536, 813, 887
BshNI GGYRCC 3 cut(s) 176, 268, 1097
BshVI ATCGAT 1 cut(s) 1008
BsiHKAI GWGCWC 1 cut(s) 529
BsiSI CCGG 1 cut(s) 1311
BslFI GGGAC 1 cut(s) 657
BslI CCNNNNNNNGG 3 cut(s) 521, 815, 1193
BsmAI GTCTC 5 cut(s) 112, 293, 706, 861, 955
BsmBI CGTCTC 1 cut(s) 706
BsmFI GGGAC 1 cut(s) 657
BsnI GGCC 4 cut(s) 298, 536, 813, 887
Bso31I GGTCTC 1 cut(s) 955
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 529, 1100
Bsp19I CCATGG 2 cut(s) 418, 814
BspACI CCGC 8 cut(s) 15, 18, 75, 113, 115, 397, 430, 450
BspANI GGCC 4 cut(s) 298, 536, 813, 887
BspCNI CTCAG 1 cut(s) 1115
BspDI ATCGAT 1 cut(s) 1008
BspFNI CGCG 4 cut(s) 15, 83, 115, 127
BspLI GGNNCC 4 cut(s) 155, 178, 270, 1099
BspPI GGATC 4 cut(s) 138, 365, 926, 1322
BspT104I TTCGAA 1 cut(s) 93
BspT107I GGYRCC 3 cut(s) 176, 268, 1097
BspTNI GGTCTC 1 cut(s) 955
BsrDI GCAATG 3 cut(s) 193, 357, 606
BssECI CCNNGG 4 cut(s) 113, 418, 814, 835
BssNI GRCGYC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 3 cut(s) 418, 814, 835
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 1 cut(s) 810
Bst4CI ACNGT 7 cut(s) 123, 439, 457, 733, 877, 1196, 1299
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 1090
BstBI TTCGAA 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 1102, 1291
BstDSI CCRYGG 3 cut(s) 113, 418, 814
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 471
BstFNI CGCG 4 cut(s) 15, 83, 115, 127
BstHHI GCGC 1 cut(s) 129
BstMAI GTCTC 5 cut(s) 112, 293, 706, 861, 955
BstMWI GCNNNNNNNGC 4 cut(s) 74, 106, 533, 831
BstNI CCWGG 1 cut(s) 810
BstSCI CCNGG 2 cut(s) 808, 1310
BstSLI GKGCMC 1 cut(s) 1100
BstUI CGCG 4 cut(s) 15, 83, 115, 127
BstV1I GCAGC 1 cut(s) 1146
BstV2I GAAGAC 1 cut(s) 236
BstX2I RGATCY 5 cut(s) 143, 342, 488, 780, 1125
BstXI CCANNNNNNTGG 3 cut(s) 265, 347, 381
BstYI RGATCY 5 cut(s) 143, 342, 488, 780, 1125
Bsu15I ATCGAT 1 cut(s) 1008
BsuRI GGCC 4 cut(s) 298, 536, 813, 887
BsuTUI ATCGAT 1 cut(s) 1008
BtgI CCRYGG 3 cut(s) 113, 418, 814
BtsCI GGATG 1 cut(s) 471
BtsI GCAGTG 1 cut(s) 900
BtsIMutI CAGTG 2 cut(s) 453, 900
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 2 cut(s) 314, 806
Cfr42I CCGCGG 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 1008
CseI GACGC 2 cut(s) 181, 688
Csp6I GTAC 3 cut(s) 21, 351, 1255
CspCI CAANNNNNGTGG 2 cut(s) 461, 496
CviAII CATG 4 cut(s) 419, 815, 927, 1149
CviQI GTAC 3 cut(s) 21, 351, 1255
DdeI CTNAG 2 cut(s) 1102, 1291
DraI TTTAAA 1 cut(s) 648
EaeI YGGCCR 2 cut(s) 811, 885
Eco130I CCWWGG 3 cut(s) 418, 814, 835
Eco31I GGTCTC 1 cut(s) 955
Eco47I GGWCC 2 cut(s) 314, 806
Eco72I CACGTG 1 cut(s) 1090
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoRI GAATTC 2 cut(s) 85, 687
EcoRII CCWGG 1 cut(s) 808
EcoT14I CCWWGG 3 cut(s) 418, 814, 835
ErhI CCWWGG 3 cut(s) 418, 814, 835
Esp3I CGTCTC 1 cut(s) 706
FaeI CATG 4 cut(s) 422, 818, 930, 1152
FaqI GGGAC 1 cut(s) 657
FatI CATG 4 cut(s) 418, 814, 926, 1148
FauI CCCGC 1 cut(s) 120
Fnu4HI GCNGC 4 cut(s) 16, 430, 451, 1135
FokI GGATG 1 cut(s) 478
Fsp4HI GCNGC 4 cut(s) 16, 430, 451, 1135
FspBI CTAG 5 cut(s) 47, 669, 836, 1029, 1239
GlaI GCGC 1 cut(s) 128
GluI GCNGC 4 cut(s) 16, 430, 451, 1135
GsuI CTGGAG 2 cut(s) 89, 323
HaeIII GGCC 4 cut(s) 298, 536, 813, 887
HapII CCGG 1 cut(s) 1311
HgaI GACGC 2 cut(s) 181, 688
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 422, 818, 930, 1152
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 2 cut(s) 460, 596
HindII GTYRAC 2 cut(s) 460, 596
HindIII AAGCTT 2 cut(s) 1227, 1285
HinfI GANTC 6 cut(s) 95, 261, 330, 1025, 1045, 1211
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 1 cut(s) 1311
HphI GGTGA 3 cut(s) 187, 1137, 1208
Hpy166II GTNNAC 5 cut(s) 460, 596, 1087, 1145, 1255
Hpy188I TCNGA 5 cut(s) 148, 898, 918, 1050, 1276
Hpy188III TCNNGA 6 cut(s) 312, 340, 374, 976, 1123, 1181
Hpy8I GTNNAC 5 cut(s) 460, 596, 1087, 1145, 1255
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 302, 380, 437
HpyCH4III ACNGT 7 cut(s) 123, 439, 457, 733, 877, 1196, 1299
HpyCH4IV ACGT 2 cut(s) 162, 1089
HpyCH4V TGCA 7 cut(s) 218, 495, 555, 604, 753, 1134, 1325
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 106, 533, 831
HpyF3I CTNAG 2 cut(s) 1102, 1291
HpySE526I ACGT 2 cut(s) 162, 1089
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 422, 818, 930, 1152
HspAI GCGC 1 cut(s) 127
KspAI GTTAAC 1 cut(s) 460
KspI CCGCGG 1 cut(s) 116
LmnI GCTCC 6 cut(s) 105, 114, 159, 367, 532, 664
Lsp1109I GCAGC 1 cut(s) 1146
LweI GCATC 2 cut(s) 211, 1115
MaeI CTAG 5 cut(s) 47, 669, 836, 1029, 1239
MaeII ACGT 2 cut(s) 162, 1089
MaeIII GTNAC 4 cut(s) 28, 39, 193, 631
MboII GAAGA 3 cut(s) 234, 241, 869
MflI RGATCY 5 cut(s) 143, 342, 488, 780, 1125
MhlI GDGCHC 2 cut(s) 529, 1100
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 5 cut(s) 85, 169, 277, 687, 957
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 2 cut(s) 324, 1220
MmeI TCCRAC 2 cut(s) 534, 554
Mox20I TGGCCA 1 cut(s) 813
MroXI GAANNNNTTC 1 cut(s) 89
MscI TGGCCA 1 cut(s) 813
MseI TTAA 7 cut(s) 393, 434, 459, 539, 614, 647, 765
MslI CAYNNNNRTG 1 cut(s) 977
Msp20I TGGCCA 1 cut(s) 813
MspA1I CMGCKG 2 cut(s) 115, 621
MspI CCGG 1 cut(s) 1311
MspR9I CCNGG 2 cut(s) 810, 1312
MvaI CCWGG 1 cut(s) 810
MvnI CGCG 4 cut(s) 15, 83, 115, 127
MwoI GCNNNNNNNGC 4 cut(s) 74, 106, 533, 831
NciI CCSGG 1 cut(s) 1312
NcoI CCATGG 2 cut(s) 418, 814
NlaIII CATG 4 cut(s) 422, 818, 930, 1152
NlaIV GGNNCC 4 cut(s) 155, 178, 270, 1099
NmuCI GTSAC 3 cut(s) 28, 193, 631
NspV TTCGAA 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 4 cut(s) 95, 261, 1025, 1045
PfoI TCCNGGA 1 cut(s) 1310
PkrI GCNGC 4 cut(s) 17, 431, 452, 1136
PleI GAGTC 2 cut(s) 324, 1219
PmaCI CACGTG 1 cut(s) 1090
PmlI CACGTG 1 cut(s) 1090
PpsI GAGTC 2 cut(s) 324, 1219
Ppu21I YACGTR 1 cut(s) 1090
Psp6I CCWGG 1 cut(s) 808
PspCI CACGTG 1 cut(s) 1090
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 4 cut(s) 155, 178, 270, 1099
PspPI GGNCC 2 cut(s) 314, 806
PsuI RGATCY 5 cut(s) 143, 342, 488, 780, 1125
PvuII CAGCTG 1 cut(s) 621
RsaI GTAC 3 cut(s) 22, 352, 1256
RsaNI GTAC 3 cut(s) 21, 351, 1255
RseI CAYNNNNRTG 1 cut(s) 977
SacII CCGCGG 1 cut(s) 116
SaqAI TTAA 7 cut(s) 393, 434, 459, 539, 614, 647, 765
SatI GCNGC 4 cut(s) 16, 430, 451, 1135
Sau96I GGNCC 2 cut(s) 314, 806
SchI GAGTC 2 cut(s) 324, 1220
ScrFI CCNGG 2 cut(s) 810, 1312
SduI GDGCHC 2 cut(s) 529, 1100
SfaNI GCATC 2 cut(s) 211, 1115
Sfr303I CCGCGG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 116
SinI GGWCC 2 cut(s) 314, 806
SmiMI CAYNNNNRTG 1 cut(s) 977
SpeI ACTAGT 2 cut(s) 46, 668
Sse9I AATT 5 cut(s) 85, 169, 277, 687, 957
SsiI CCGC 8 cut(s) 15, 18, 75, 113, 115, 397, 430, 450
SspMI CTAG 5 cut(s) 47, 669, 836, 1029, 1239
StyD4I CCNGG 2 cut(s) 808, 1310
StyI CCWWGG 3 cut(s) 418, 814, 835
TaaI ACNGT 7 cut(s) 123, 439, 457, 733, 877, 1196, 1299
TaiI ACGT 2 cut(s) 165, 1092
TaqI TCGA 5 cut(s) 93, 131, 520, 758, 1008
TasI AATT 5 cut(s) 85, 169, 277, 687, 957
TauI GCSGC 3 cut(s) 18, 432, 453
TfiI GAWTC 4 cut(s) 95, 261, 1025, 1045
Tru1I TTAA 7 cut(s) 393, 434, 459, 539, 614, 647, 765
Tru9I TTAA 7 cut(s) 393, 434, 459, 539, 614, 647, 765
TscAI CASTG 2 cut(s) 460, 907
TseFI GTSAC 3 cut(s) 28, 193, 631
TseI GCWGC 1 cut(s) 1134
Tsp45I GTSAC 3 cut(s) 28, 193, 631
TspDTI ATGAA 1 cut(s) 995
TspRI CASTG 2 cut(s) 460, 907
VpaK11BI GGWCC 2 cut(s) 314, 806
XagI CCTNNNNNAGG 1 cut(s) 519
XapI RAATTY 2 cut(s) 85, 687
XmaJI CCTAGG 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 5 cut(s) 47, 669, 836, 1029, 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.