RLG00000019531

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009481
Physical Location & Seq
Reverse (-)
53216060 .. 53219313
3254 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019531

Sequence Viewer

Length: 1383 bp
ATGAAAACCCTTCGATTCTACTCATCATCTTCTTCTTCTTCTTCCACTTGTCTCATTTTTCTACTTCATATATTCTCTCTCTTTCTGAGCTCAAATACTGCTTCTACTACTGATAGAGAGAAATTTGACTCTCTCTTACGGCTCCCCCAATCCGGGTCTACCAGAACCCGACATAGATCCAAGAGGGTTCTCTTTGTTGGCGATTTTGGTGCCAAAGGAGATGGCATCACTGATGATTCTAAGGCTTTCAAAAATGCTTGGGAGGTTGCTTGTTCTATTCCCACACGGACAAGAATTCTAATTCCCTCCGGGAGGACTTACCTTATCCATCCTATTGATATTGCTGGACCTTGTCGATCAAGGGTGACTTTAATGATCTCTGGTACCATCATTGCTCCCATCAATCCTGATGCTTGGATTGGTTTGAATCGACGCAAGTGGCTTTATTTCCATGGGGTGAACCATCTCACCATTGAAGGGGGAGGAACAGTCGATGGGATGGGACAGGAATGGTGGGCTCGTTCTTGTAAGACCAACTCAACAAATCCATGTCGACATGCTCCAACGGCCATTACATTCCATAGGTGCAAGAATTTGAAGGTCAAAAATATCATGATTGTCAATAGTCAAAAAATGCACATGGCATTCACCAACTGTATGCGGGTATTAGCATCCCATCTCCAAGTTATTGCACCTGCCACTAGTCCCAATACTGATGGAATCCACATTAGTGTGTCAAAGGGTGTTGAGGTCAAGGATAGCATAATAAGAACAGGAGATGACTGCATTTCTATAGTTAGCAATTCTTCACGCATCCGGGTCGTGAATATTGTCTGCGGCCCTGGTCATGGCATAAGCATCGGAAGCTTGGGAAAATCAAACTCTTGGTCACAAGTGCGTGATGTAGTAGTTGATGCAGCATTCCTCTCTAACACTGAGAATGGAGTGAGGATCAAAACATGGCAGGGAGGTAGTGGTTTTGCTTCTAACATTGCATTTCGGAATGTGGTCATGGACAACGTATCAAATCCAATTATAATTGATCAATATTATTGTGACTCCCAGTTGCCATGTTTAAATCAGACTTTGGCAGTCAAAGTTGAGAATATATCCTTTATGCACATTAAAGGGACTTCTGCTACAGACGAAGCAATACGATTTGCATGCAGTGATGATTCTCCATGTGAAGGTATATATTTAGAGGATGTTCAACTTCTACCATCCTATGGTGAAATAACAAGATCATTTTGTTGGGCAGCTCAAGGCTCAAGTTTGGGTTCAGTTGACCCACCTGCATGCTTTTCCGATAGTGAAGAATTCATTAAACAAAAAGTCCTCATACACCCTGTAAGTTTGGAATATGGAGGAAAGGCAACCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

461

Amino Acids

50.35

Weight (kDa)

8.84

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 91 - 406 1.1e-96 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1037
AarI CACCTGC 2 cut(s) 703, 1300
Acc36I ACCTGC 2 cut(s) 703, 1300
Acc65I GGTACC 1 cut(s) 383
AccB1I GGYRCC 2 cut(s) 209, 383
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccI GTMKAC 2 cut(s) 158, 553
AciI CCGC 2 cut(s) 661, 837
AclWI GGATC 2 cut(s) 171, 959
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 4 cut(s) 122, 294, 592, 1316
AfaI GTAC 1 cut(s) 385
AfiI CCNNNNNNNGG 7 cut(s) 152, 153, 312, 477, 848, 1187, 1226
AgsI TTSAA 5 cut(s) 250, 427, 476, 598, 1211
AhlI ACTAGT 1 cut(s) 701
AjnI CCWGG 1 cut(s) 841
AleI CACNNNNGTG 1 cut(s) 729
AluBI AGCT 3 cut(s) 90, 867, 1259
AluI AGCT 3 cut(s) 90, 867, 1259
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 2 cut(s) 171, 959
AoxI GGCC 2 cut(s) 567, 838
ApeKI GCWGC 2 cut(s) 917, 1256
ApoI RAATTY 4 cut(s) 122, 294, 592, 1316
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
Asp718I GGTACC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 347, 839
AsuC2I CCSGG 3 cut(s) 154, 310, 818
AsuHPI GGTGA 5 cut(s) 376, 460, 469, 640, 1241
AvaII GGWCC 1 cut(s) 347
BanI GGYRCC 2 cut(s) 209, 383
BanII GRGCYC 2 cut(s) 92, 520
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 929, 1268
BceAI ACGGC 2 cut(s) 155, 582
BcgI CGANNNNNNTGC 8 cut(s) 191, 225, 802, 836, 841, 875, 1146, 1180
BciT130I CCWGG 1 cut(s) 843
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 3 cut(s) 154, 310, 818
BcoDI GTCTC 1 cut(s) 56
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 3 cut(s) 792, 1140, 1379
BfuAI ACCTGC 2 cut(s) 703, 1300
BisI GCNGC 3 cut(s) 838, 918, 1257
BlsI GCNGC 3 cut(s) 839, 919, 1258
Bme1390I CCNGG 4 cut(s) 154, 310, 818, 843
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 347, 839
BmiI GGNNCC 3 cut(s) 143, 211, 385
BmrFI CCNGG 4 cut(s) 154, 310, 818, 843
BmrI ACTGGG 1 cut(s) 1057
BmsI GCATC 6 cut(s) 234, 400, 680, 822, 867, 904
BmuI ACTGGG 1 cut(s) 1057
BpuEI CTTGAG 2 cut(s) 1245, 1252
BpuMI CCSGG 3 cut(s) 154, 310, 818
BsaJI CCNNGG 2 cut(s) 451, 841
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 7 cut(s) 152, 153, 312, 477, 848, 1187, 1226
Bse1I ACTGG 1 cut(s) 1063
Bse3DI GCAATG 2 cut(s) 390, 990
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 2 cut(s) 451, 841
BseGI GGATG 6 cut(s) 328, 504, 671, 813, 1210, 1220
BseLI CCNNNNNNNGG 7 cut(s) 152, 153, 312, 477, 848, 1187, 1226
BseMI GCAATG 2 cut(s) 390, 990
BseMII CTCAG 2 cut(s) 77, 927
BseNI ACTGG 1 cut(s) 1063
BseXI GCAGC 2 cut(s) 929, 1268
BshFI GGCC 2 cut(s) 569, 840
BshNI GGYRCC 2 cut(s) 209, 383
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 3 cut(s) 153, 309, 817
BslFI GGGAC 3 cut(s) 516, 690, 1144
BslI CCNNNNNNNGG 7 cut(s) 152, 153, 312, 477, 848, 1187, 1226
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 3 cut(s) 516, 690, 1144
BsmI GAATGC 2 cut(s) 644, 920
BsnI GGCC 2 cut(s) 569, 840
Bsp1286I GDGCHC 2 cut(s) 92, 520
Bsp143I GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
Bsp19I CCATGG 1 cut(s) 451
BspACI CCGC 2 cut(s) 661, 837
BspANI GGCC 2 cut(s) 569, 840
BspCNI CTCAG 2 cut(s) 78, 928
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 3 cut(s) 143, 211, 385
BspMI ACCTGC 2 cut(s) 703, 1300
BspPI GGATC 2 cut(s) 171, 959
BspT107I GGYRCC 2 cut(s) 209, 383
BsrDI GCAATG 2 cut(s) 390, 990
BsrI ACTGG 1 cut(s) 1063
BssECI CCNNGG 2 cut(s) 451, 841
BssMI GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 2 cut(s) 490, 656
BstC8I GCNNGC 2 cut(s) 1165, 1297
BstDEI CTNAG 3 cut(s) 86, 240, 936
BstDSI CCRYGG 1 cut(s) 451
BstENI CCTNNNNNAGG 1 cut(s) 310
BstF5I GGATG 6 cut(s) 328, 504, 671, 813, 1210, 1220
BstKTI GATC 6 cut(s) 179, 359, 378, 954, 1045, 1244
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
BstMWI GCNNNNNNNGC 2 cut(s) 566, 864
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 3 cut(s) 560, 1167, 1299
BstSCI CCNGG 4 cut(s) 152, 308, 816, 841
BstSFI CTRYAG 3 cut(s) 792, 1140, 1379
BstV1I GCAGC 2 cut(s) 929, 1268
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 2 cut(s) 569, 840
BtgI CCRYGG 1 cut(s) 451
BtsCI GGATG 6 cut(s) 328, 504, 671, 813, 1210, 1220
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 3 cut(s) 228, 933, 1174
BveI ACCTGC 2 cut(s) 703, 1300
Cac8I GCNNGC 2 cut(s) 1165, 1297
CciI TCATGA 1 cut(s) 612
Cfr13I GGNCC 2 cut(s) 347, 839
CseI GACGC 1 cut(s) 441
Csp6I GTAC 1 cut(s) 384
CviQI GTAC 1 cut(s) 384
DdeI CTNAG 3 cut(s) 86, 240, 936
DpnI GATC 6 cut(s) 178, 358, 377, 953, 1044, 1243
DpnII GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
DraI TTTAAA 1 cut(s) 1077
EaeI YGGCCR 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 92, 520
Eco47I GGWCC 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 90
EcoICRI GAGCTC 1 cut(s) 90
EcoNI CCTNNNNNAGG 1 cut(s) 310
EcoRI GAATTC 2 cut(s) 294, 1316
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 92, 520
ErhI CCWWGG 1 cut(s) 451
FalI AAGNNNNNCTT 2 cut(s) 352, 384
FaqI GGGAC 3 cut(s) 516, 690, 1144
FauI CCCGC 1 cut(s) 654
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 2 cut(s) 158, 553
Fnu4HI GCNGC 3 cut(s) 838, 918, 1257
FokI GGATG 6 cut(s) 315, 511, 658, 800, 1207, 1217
FriOI GRGCYC 2 cut(s) 92, 520
Fsp4HI GCNGC 3 cut(s) 838, 918, 1257
FspBI CTAG 1 cut(s) 702
GluI GCNGC 3 cut(s) 838, 918, 1257
HaeIII GGCC 2 cut(s) 569, 840
HapII CCGG 3 cut(s) 153, 309, 817
HgaI GACGC 1 cut(s) 441
HincII GTYRAC 2 cut(s) 554, 1285
HindII GTYRAC 2 cut(s) 554, 1285
HindIII AAGCTT 1 cut(s) 865
HinfI GANTC 7 cut(s) 15, 128, 236, 427, 720, 1058, 1175
HpaII CCGG 3 cut(s) 153, 309, 817
HphI GGTGA 5 cut(s) 376, 460, 469, 640, 1241
Hpy166II GTNNAC 4 cut(s) 159, 460, 554, 1285
Hpy188I TCNGA 5 cut(s) 87, 863, 1002, 1083, 1306
Hpy188III TCNNGA 3 cut(s) 407, 613, 823
Hpy8I GTNNAC 4 cut(s) 159, 460, 554, 1285
Hpy99I CGWCG 1 cut(s) 435
HpyAV CCTTC 4 cut(s) 20, 470, 592, 1181
HpyCH4III ACNGT 2 cut(s) 490, 656
HpyCH4IV ACGT 1 cut(s) 1020
HpyF10VI GCNNNNNNNGC 2 cut(s) 566, 864
HpyF3I CTNAG 3 cut(s) 86, 240, 936
HpySE526I ACGT 1 cut(s) 1020
KpnI GGTACC 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
LmnI GCTCC 3 cut(s) 147, 400, 565
Lsp1109I GCAGC 2 cut(s) 929, 1268
LweI GCATC 6 cut(s) 234, 400, 680, 822, 867, 904
MaeI CTAG 1 cut(s) 702
MaeII ACGT 1 cut(s) 1020
MaeIII GTNAC 3 cut(s) 364, 888, 1055
MalI GATC 6 cut(s) 178, 358, 377, 953, 1044, 1243
MboI GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
MboII GAAGA 7 cut(s) 21, 24, 27, 30, 33, 798, 1325
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 2 cut(s) 92, 520
MluCI AATT 8 cut(s) 122, 294, 300, 592, 802, 1032, 1038, 1316
MlyI GAGTC 2 cut(s) 122, 1052
MmeI TCCRAC 1 cut(s) 587
MseI TTAA 4 cut(s) 371, 1076, 1125, 1323
MslI CAYNNNNRTG 3 cut(s) 729, 731, 1294
MspI CCGG 3 cut(s) 153, 309, 817
MspR9I CCNGG 4 cut(s) 154, 310, 818, 843
Mva1269I GAATGC 2 cut(s) 644, 920
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 2 cut(s) 566, 864
NciI CCSGG 3 cut(s) 154, 310, 818
NcoI CCATGG 1 cut(s) 451
NdeII GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
NlaIV GGNNCC 3 cut(s) 143, 211, 385
NmuCI GTSAC 3 cut(s) 364, 888, 1055
NspI RCATGY 3 cut(s) 560, 1167, 1299
OliI CACNNNNGTG 1 cut(s) 729
PaeI GCATGC 2 cut(s) 1167, 1299
PagI TCATGA 1 cut(s) 612
PaqCI CACCTGC 2 cut(s) 703, 1300
PctI GAATGC 2 cut(s) 644, 920
PfeI GAWTC 5 cut(s) 15, 236, 427, 720, 1175
PflFI GACNNNGTC 1 cut(s) 351
PflMI CCANNNNNTGG 1 cut(s) 1226
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 3 cut(s) 839, 919, 1258
PleI GAGTC 2 cut(s) 122, 1052
PpsI GAGTC 2 cut(s) 122, 1052
PsiI TTATAA 1 cut(s) 1037
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 3 cut(s) 143, 211, 385
PspPI GGNCC 2 cut(s) 347, 839
PsuI RGATCY 1 cut(s) 176
PsyI GACNNNGTC 1 cut(s) 351
RsaI GTAC 1 cut(s) 385
RsaNI GTAC 1 cut(s) 384
RseI CAYNNNNRTG 3 cut(s) 729, 731, 1294
SacI GAGCTC 1 cut(s) 92
SalI GTCGAC 1 cut(s) 552
SaqAI TTAA 4 cut(s) 371, 1076, 1125, 1323
SatI GCNGC 3 cut(s) 838, 918, 1257
Sau3AI GATC 6 cut(s) 176, 356, 375, 951, 1042, 1241
Sau96I GGNCC 2 cut(s) 347, 839
SchI GAGTC 2 cut(s) 122, 1052
ScrFI CCNGG 4 cut(s) 154, 310, 818, 843
SduI GDGCHC 2 cut(s) 92, 520
SfaNI GCATC 6 cut(s) 234, 400, 680, 822, 867, 904
SfcI CTRYAG 3 cut(s) 792, 1140, 1379
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 3 cut(s) 729, 731, 1294
SmlI CTYRAG 2 cut(s) 1260, 1267
SmoI CTYRAG 2 cut(s) 1260, 1267
SpeI ACTAGT 1 cut(s) 701
SphI GCATGC 2 cut(s) 1167, 1299
Sse9I AATT 8 cut(s) 122, 294, 300, 592, 802, 1032, 1038, 1316
SsiI CCGC 2 cut(s) 661, 837
SspI AATATT 2 cut(s) 829, 1049
SspMI CTAG 1 cut(s) 702
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 4 cut(s) 152, 308, 816, 841
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 490, 656
TaiI ACGT 1 cut(s) 1023
TaqI TCGA 5 cut(s) 13, 355, 430, 492, 553
TasI AATT 8 cut(s) 122, 294, 300, 592, 802, 1032, 1038, 1316
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 5 cut(s) 15, 236, 427, 720, 1175
Tru1I TTAA 4 cut(s) 371, 1076, 1125, 1323
Tru9I TTAA 4 cut(s) 371, 1076, 1125, 1323
TscAI CASTG 3 cut(s) 235, 940, 1174
TseFI GTSAC 3 cut(s) 364, 888, 1055
TseI GCWGC 2 cut(s) 917, 1256
Tsp45I GTSAC 3 cut(s) 364, 888, 1055
TspDTI ATGAA 3 cut(s) 17, 56, 1309
TspGWI ACGGA 1 cut(s) 301
TspRI CASTG 3 cut(s) 235, 940, 1174
Tth111I GACNNNGTC 1 cut(s) 351
Van91I CCANNNNNTGG 1 cut(s) 1226
VpaK11BI GGWCC 1 cut(s) 347
XagI CCTNNNNNAGG 1 cut(s) 310
XapI RAATTY 4 cut(s) 122, 294, 592, 1316
XceI RCATGY 3 cut(s) 560, 1167, 1299
XmiI GTMKAC 2 cut(s) 158, 553
XspI CTAG 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.