Rroxscaffold_2G00108290

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009481
Physical Location & Seq
Forward (+)
32252406 .. 32255943
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108290.1

Sequence Viewer

Length: 1299 bp
ATGAAAACCCTTCGATTCCACTCATCTTCTTCTTCCACTTGTCTCATTTTTCTCCTTCATATATTCTCTCTCTTCCTGAGCTCAAATACTACTCCTACTACTGATAGAGAGAAATTTGACTCTCTCTTACGGCTCCCCCAATCTGGGTCTACCAGAACTCGACTTAGATCCAAGAGGGTTCTCTTTGTTGGCGATTTTGGTGCCAAAGGAGATGGCATCACTGATGATTCTCAGGCTTTCAAAAATGCTTGGGAGGTTGCTTGTTCTATTCCCACACGGACAAGAATTCTAATTCCCTCCGGGAGGACTTACCTTATCCATCCTGTTGATATTGCTGGACCTTGTCGATCAAAGGTGACTTTAATGATCTCTGGTACCATCATTGCTCCCATCAATCCTGATGCTTGGATTGGTTTGAATCGACGCAAGTGGCTTTATTTCCATGGGGTGAACCATCTCACCATTGAAGGGGGAGGAACAGTCGATGGGATGGGACAGGAATGCCATGTCGACATGCTCCAACGGTGCAAAAATTTGAAGGTCAAGAATCTTATGATTGTCAATAGTCAAAAAATGCACATGGCATTCACCAACTGTATGCGGGTATTGGCATCCCATCTCCAAGTTATTGCACCTGCCACTAGTCCCAATACTGATGGAATCCACATTAGTGTGTCGAAGGGTGTTGAGGTCAAGGATAGCATAATTAGAACAGGAGATGACTGCATTTCTATAGTTAGCAATTCTTCACGCATCCGGATCGTGAATATTGTCTGTGGCCCTGGTCATGGCATTAGCTTGGGAAAATCAAACTCTTGGTCACAAGTGCGTGATGTAATGGTTGATGCAGCATTCCTCTCTAACACTGAGAATGGAGTGAGGATCAAGACATGGCAGGGAGGTAGTGGTTTTGCCTCAAACATTGCATTTCGGAATGTGGTCATGGACAACGTATCAAATCCAATTATAATTGATCAATATTACTGTGACTCCCAGTTGCCATGTTTAAATCAGACTTTGGCGGTCAAAGTTGAGAATATATCCTTTATGCACATTAAAGGGACTTCTGCTACAGACGAAGCGATACGATTTGCATGCAGTGATGATTCTCCATGTGAAGGTTTATATTTAGAAGATGTTCAACTTCTACCATCCAATGGTGAAATAACAAGATCATTTTGTTGGGCAGCTCAAGGCTCAAGTTTGGGTTCAGTTGACCCACCTGCATGCTTTTCCAATAGTGAAGAATTCATTAAACAAAAAGTCCTCATACAGCCTGTAAGTTTGGAATATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

432

Amino Acids

47.36

Weight (kDa)

8.35

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 88 - 384 4.8e-77 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 968
AarI CACCTGC 2 cut(s) 643, 1231
Acc36I ACCTGC 2 cut(s) 643, 1231
Acc65I GGTACC 1 cut(s) 374
AccB1I GGYRCC 2 cut(s) 200, 374
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccI GTMKAC 2 cut(s) 149, 510
AccIII TCCGGA 1 cut(s) 756
AciI CCGC 2 cut(s) 601, 1022
AclWI GGATC 3 cut(s) 162, 767, 890
AcsI RAATTY 4 cut(s) 113, 285, 532, 1247
AfaI GTAC 1 cut(s) 376
AfiI CCNNNNNNNGG 7 cut(s) 143, 144, 303, 468, 788, 1118, 1157
AgsI TTSAA 5 cut(s) 241, 418, 467, 538, 1142
AhlI ACTAGT 1 cut(s) 641
AjnI CCWGG 1 cut(s) 781
AleI CACNNNNGTG 1 cut(s) 669
AluBI AGCT 3 cut(s) 81, 798, 1190
AluI AGCT 3 cut(s) 81, 798, 1190
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 3 cut(s) 162, 767, 890
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 1 cut(s) 778
ApeKI GCWGC 2 cut(s) 848, 1187
ApoI RAATTY 4 cut(s) 113, 285, 532, 1247
ArsI GACNNNNNNTTYG 2 cut(s) 803, 835
Asp700I GAANNNNTTC 1 cut(s) 1137
Asp718I GGTACC 1 cut(s) 374
AspS9I GGNCC 2 cut(s) 338, 779
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 5 cut(s) 367, 451, 460, 580, 1172
AvaII GGWCC 1 cut(s) 338
BanI GGYRCC 2 cut(s) 200, 374
BanII GRGCYC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 860, 1199
BceAI ACGGC 1 cut(s) 146
BcgI CGANNNNNNTGC 6 cut(s) 182, 216, 742, 776, 1077, 1111
BciT130I CCWGG 1 cut(s) 783
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 47
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 2 cut(s) 732, 1071
BfuAI ACCTGC 2 cut(s) 643, 1231
BisI GCNGC 2 cut(s) 849, 1188
BlsI GCNGC 2 cut(s) 850, 1189
Bme1390I CCNGG 2 cut(s) 301, 783
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 338, 779
BmiI GGNNCC 3 cut(s) 134, 202, 376
BmrFI CCNGG 2 cut(s) 301, 783
BmrI ACTGGG 1 cut(s) 988
BmsI GCATC 5 cut(s) 225, 391, 620, 762, 835
BmuI ACTGGG 1 cut(s) 988
Bpu10I CCTNAGC 1 cut(s) 77
BpuEI CTTGAG 2 cut(s) 1176, 1183
BpuMI CCSGG 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 442, 781
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 974, 1004
Bsc4I CCNNNNNNNGG 7 cut(s) 143, 144, 303, 468, 788, 1118, 1157
Bse1I ACTGG 1 cut(s) 994
Bse3DI GCAATG 2 cut(s) 381, 921
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 1 cut(s) 783
BseDI CCNNGG 2 cut(s) 442, 781
BseGI GGATG 5 cut(s) 319, 495, 611, 753, 1151
BseLI CCNNNNNNNGG 7 cut(s) 143, 144, 303, 468, 788, 1118, 1157
BseMI GCAATG 2 cut(s) 381, 921
BseMII CTCAG 3 cut(s) 68, 245, 858
BseNI ACTGG 1 cut(s) 994
BseXI GCAGC 2 cut(s) 860, 1199
BshFI GGCC 1 cut(s) 780
BshNI GGYRCC 2 cut(s) 200, 374
BsiHKAI GWGCWC 1 cut(s) 83
BsiSI CCGG 2 cut(s) 300, 757
BslFI GGGAC 3 cut(s) 507, 630, 1075
BslI CCNNNNNNNGG 7 cut(s) 143, 144, 303, 468, 788, 1118, 1157
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 3 cut(s) 507, 630, 1075
BsmI GAATGC 3 cut(s) 506, 584, 851
BsnI GGCC 1 cut(s) 780
Bsp1286I GDGCHC 1 cut(s) 83
Bsp13I TCCGGA 1 cut(s) 756
Bsp143I GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 2 cut(s) 601, 1022
BspANI GGCC 1 cut(s) 780
BspCNI CTCAG 3 cut(s) 69, 244, 859
BspEI TCCGGA 1 cut(s) 756
BspLI GGNNCC 3 cut(s) 134, 202, 376
BspMI ACCTGC 2 cut(s) 643, 1231
BspPI GGATC 3 cut(s) 162, 767, 890
BspT107I GGYRCC 2 cut(s) 200, 374
BsrDI GCAATG 2 cut(s) 381, 921
BsrI ACTGG 1 cut(s) 994
BssECI CCNNGG 2 cut(s) 442, 781
BssMI GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 4 cut(s) 481, 525, 596, 986
Bst6I CTCTTC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 1096, 1228
BstDEI CTNAG 4 cut(s) 77, 164, 231, 867
BstDSI CCRYGG 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 301
BstF5I GGATG 5 cut(s) 319, 495, 611, 753, 1151
BstKTI GATC 7 cut(s) 170, 350, 369, 762, 885, 976, 1175
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
BstNI CCWGG 1 cut(s) 783
BstNSI RCATGY 3 cut(s) 517, 1098, 1230
BstSCI CCNGG 2 cut(s) 299, 781
BstSFI CTRYAG 2 cut(s) 732, 1071
BstV1I GCAGC 2 cut(s) 860, 1199
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
BsuRI GGCC 1 cut(s) 780
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 5 cut(s) 319, 495, 611, 753, 1151
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 3 cut(s) 219, 864, 1105
BveI ACCTGC 2 cut(s) 643, 1231
Cac8I GCNNGC 2 cut(s) 1096, 1228
Cfr13I GGNCC 2 cut(s) 338, 779
CseI GACGC 1 cut(s) 432
Csp6I GTAC 1 cut(s) 375
CviJI RGCY 9 cut(s) 81, 133, 236, 433, 780, 798, 1190, 1197, 1276
CviKI_1 RGCY 9 cut(s) 81, 133, 236, 433, 780, 798, 1190, 1197, 1276
CviQI GTAC 1 cut(s) 375
DdeI CTNAG 4 cut(s) 77, 164, 231, 867
DpnI GATC 7 cut(s) 169, 349, 368, 761, 884, 975, 1174
DpnII GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
DraI TTTAAA 1 cut(s) 1008
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 81
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoRI GAATTC 2 cut(s) 285, 1247
EcoRII CCWGG 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 442
FaqI GGGAC 3 cut(s) 507, 630, 1075
FauI CCCGC 1 cut(s) 594
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 2 cut(s) 149, 510
Fnu4HI GCNGC 2 cut(s) 849, 1188
FokI GGATG 5 cut(s) 306, 502, 598, 740, 1138
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 849, 1188
FspBI CTAG 1 cut(s) 642
GluI GCNGC 2 cut(s) 849, 1188
HaeIII GGCC 1 cut(s) 780
HapII CCGG 2 cut(s) 300, 757
HgaI GACGC 1 cut(s) 432
HincII GTYRAC 2 cut(s) 511, 1216
HindII GTYRAC 2 cut(s) 511, 1216
HinfI GANTC 8 cut(s) 15, 119, 227, 418, 547, 660, 989, 1106
HpaII CCGG 2 cut(s) 300, 757
HphI GGTGA 5 cut(s) 367, 451, 460, 580, 1172
Hpy166II GTNNAC 4 cut(s) 150, 451, 511, 1216
Hpy188I TCNGA 2 cut(s) 933, 1014
Hpy188III TCNNGA 6 cut(s) 76, 398, 544, 757, 763, 886
Hpy8I GTNNAC 4 cut(s) 150, 451, 511, 1216
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 6 cut(s) 20, 65, 461, 532, 673, 1112
HpyCH4III ACNGT 4 cut(s) 481, 525, 596, 986
HpyCH4IV ACGT 1 cut(s) 951
HpyF3I CTNAG 4 cut(s) 77, 164, 231, 867
HpySE526I ACGT 1 cut(s) 951
Kpn2I TCCGGA 1 cut(s) 756
KpnI GGTACC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
LmnI GCTCC 3 cut(s) 138, 391, 522
Lsp1109I GCAGC 2 cut(s) 860, 1199
LweI GCATC 5 cut(s) 225, 391, 620, 762, 835
MaeI CTAG 1 cut(s) 642
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 3 cut(s) 355, 819, 986
MalI GATC 7 cut(s) 169, 349, 368, 761, 884, 975, 1174
MboI GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
MboII GAAGA 7 cut(s) 18, 21, 24, 64, 738, 1145, 1256
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 83
MluCI AATT 9 cut(s) 113, 285, 291, 532, 705, 742, 963, 969, 1247
MlyI GAGTC 2 cut(s) 113, 983
MmeI TCCRAC 1 cut(s) 544
MroI TCCGGA 1 cut(s) 756
MroXI GAANNNNTTC 1 cut(s) 1137
MseI TTAA 4 cut(s) 362, 1007, 1056, 1254
MslI CAYNNNNRTG 3 cut(s) 669, 671, 1225
MspI CCGG 2 cut(s) 300, 757
MspR9I CCNGG 2 cut(s) 301, 783
Mva1269I GAATGC 3 cut(s) 506, 584, 851
MvaI CCWGG 1 cut(s) 783
NciI CCSGG 1 cut(s) 301
NcoI CCATGG 1 cut(s) 442
NdeII GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
NlaIV GGNNCC 3 cut(s) 134, 202, 376
NmuCI GTSAC 3 cut(s) 355, 819, 986
NspI RCATGY 3 cut(s) 517, 1098, 1230
OliI CACNNNNGTG 1 cut(s) 669
PaeI GCATGC 2 cut(s) 1098, 1230
PaqCI CACCTGC 2 cut(s) 643, 1231
PctI GAATGC 3 cut(s) 506, 584, 851
PdmI GAANNNNTTC 1 cut(s) 1137
PfeI GAWTC 6 cut(s) 15, 227, 418, 547, 660, 1106
PflFI GACNNNGTC 1 cut(s) 342
PflMI CCANNNNNTGG 1 cut(s) 1157
PfoI TCCNGGA 1 cut(s) 299
PkrI GCNGC 2 cut(s) 850, 1189
PleI GAGTC 2 cut(s) 113, 983
PpsI GAGTC 2 cut(s) 113, 983
PsiI TTATAA 1 cut(s) 968
Psp124BI GAGCTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 781
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 3 cut(s) 134, 202, 376
PspPI GGNCC 2 cut(s) 338, 779
PsuI RGATCY 1 cut(s) 167
PsyI GACNNNGTC 1 cut(s) 342
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 3 cut(s) 669, 671, 1225
SacI GAGCTC 1 cut(s) 83
SalI GTCGAC 1 cut(s) 509
SaqAI TTAA 4 cut(s) 362, 1007, 1056, 1254
SatI GCNGC 2 cut(s) 849, 1188
Sau3AI GATC 7 cut(s) 167, 347, 366, 759, 882, 973, 1172
Sau96I GGNCC 2 cut(s) 338, 779
SchI GAGTC 2 cut(s) 113, 983
ScrFI CCNGG 2 cut(s) 301, 783
SduI GDGCHC 1 cut(s) 83
SfaNI GCATC 5 cut(s) 225, 391, 620, 762, 835
SfcI CTRYAG 2 cut(s) 732, 1071
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 3 cut(s) 669, 671, 1225
SmlI CTYRAG 2 cut(s) 1191, 1198
SmoI CTYRAG 2 cut(s) 1191, 1198
SpeI ACTAGT 1 cut(s) 641
SphI GCATGC 2 cut(s) 1098, 1230
Sse9I AATT 9 cut(s) 113, 285, 291, 532, 705, 742, 963, 969, 1247
SsiI CCGC 2 cut(s) 601, 1022
SspI AATATT 2 cut(s) 769, 980
SspMI CTAG 1 cut(s) 642
SstI GAGCTC 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 299, 781
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 4 cut(s) 481, 525, 596, 986
TaiI ACGT 1 cut(s) 954
TaqI TCGA 7 cut(s) 13, 160, 346, 421, 483, 510, 677
TasI AATT 9 cut(s) 113, 285, 291, 532, 705, 742, 963, 969, 1247
TfiI GAWTC 6 cut(s) 15, 227, 418, 547, 660, 1106
Tru1I TTAA 4 cut(s) 362, 1007, 1056, 1254
Tru9I TTAA 4 cut(s) 362, 1007, 1056, 1254
TscAI CASTG 3 cut(s) 226, 871, 1105
TseFI GTSAC 3 cut(s) 355, 819, 986
TseI GCWGC 2 cut(s) 848, 1187
Tsp45I GTSAC 3 cut(s) 355, 819, 986
TspDTI ATGAA 3 cut(s) 17, 47, 1240
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 3 cut(s) 226, 871, 1105
Tth111I GACNNNGTC 1 cut(s) 342
Van91I CCANNNNNTGG 1 cut(s) 1157
VpaK11BI GGWCC 1 cut(s) 338
XagI CCTNNNNNAGG 1 cut(s) 301
XapI RAATTY 4 cut(s) 113, 285, 532, 1247
XceI RCATGY 3 cut(s) 517, 1098, 1230
XmiI GTMKAC 2 cut(s) 149, 510
XmnI GAANNNNTTC 1 cut(s) 1137
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.