Rh2BG388100

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009481
Physical Location & Seq
Reverse (-)
55266522 .. 55271231
4710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG388100.1

Sequence Viewer

Length: 1011 bp
ATGATCTCTGGTACCATCATTGCTCCCATCAATCCTGATGCTTGGATTGGTTTGAATCGACGCAAGTGGCTTTATTTCCATGGGGTGAACCATCTCACCATTGAAGGGGGAGGAACAGTCGATGGGATGGGACAGGAATGGTGGGCTCGTTCTTGTAAGACCAACTCAACAAATCCATGTCGACATGCTCCAACGGCCATTACATTCCATAGGTGCAAGAATTTGAAGGTCAAAAATATTATGATTGTCAATAGTCAAAAAATGCACATGGCATTCACCAACTGTATGCGGGTATTGGCATCCCATCTCCAAGTTATTGCACCTGCCACTAGTCCCAATACTGATGGAATCCACATTAGTGTGTCAAAGGGTGTTGAGGTCAAGGATAGCATAATAAGAACAGGAGATGACTGCATTTCTATAGTTAGCAATTCTTCACGCATCCGGATCGTGAATATTGTCTGCGGCCCTGGTCATGGCATAAGCATCGGAAGCTTGGGAAAATCAAACTCTTGGTCACATGTGCGTGATGTAGTGGTTGATGCAGCATTCCTCTCTAACACTGAGAATGGAGTGAGGATCAAAACATGGCAGGGAGGTAGTGGTTTTGCCTCTAACATTGCATTTCGGAATGTGGTCATGGACAACGTATCAAATCCAATTATAATTGATCAATATTATTGTGACTCCCAGTTGCCATGTTTAAATCAGACTTTGGCGGTCAAAGTTGAGAATATATCCTTTATACACATTAAAGGGACTTCTGCTACAGACGAAGCAATACGATTTGCATGCAGTGATGATTCTCCATGTGAAGGTTTATATTTAGAAGATGTTCAACTTCTACCATCCAATGGTGAAATAACAAGATCATTTTGTTGGGCAGCTCAAGGCTCAAGTTTGGGTTCAGTTGACCCACCTGCATGCTTTTCCGATAGTGAAGAATTCATTAAACAAAAAGTCCTCATACACCCTGTAAGTTTGGAATATGGAGGAAAGGCAATCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

336

Amino Acids

36.56

Weight (kDa)

7.13

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 21 - 283 1.2e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 665
AarI CACCTGC 2 cut(s) 331, 928
Acc36I ACCTGC 2 cut(s) 331, 928
Acc65I GGTACC 1 cut(s) 11
AccB1I GGYRCC 1 cut(s) 11
AccB7I CCANNNNNTGG 1 cut(s) 854
AccI GTMKAC 1 cut(s) 181
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 3 cut(s) 289, 465, 719
AclWI GGATC 2 cut(s) 455, 587
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 2 cut(s) 220, 944
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 4 cut(s) 105, 476, 815, 854
AflIII ACRYGT 1 cut(s) 520
AgsI TTSAA 4 cut(s) 55, 104, 226, 839
AhlI ACTAGT 1 cut(s) 329
AjnI CCWGG 1 cut(s) 469
AleI CACNNNNGTG 1 cut(s) 357
AluBI AGCT 2 cut(s) 495, 887
AluI AGCT 2 cut(s) 495, 887
AlwI GGATC 2 cut(s) 455, 587
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 2 cut(s) 195, 466
ApeKI GCWGC 2 cut(s) 545, 884
ApoI RAATTY 2 cut(s) 220, 944
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
Asp700I GAANNNNTTC 1 cut(s) 834
Asp718I GGTACC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 467
AsuHPI GGTGA 4 cut(s) 88, 97, 268, 869
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 557, 896
BccI CCATC 8 cut(s) 23, 35, 99, 116, 121, 312, 338, 856
BceAI ACGGC 1 cut(s) 210
BcgI CGANNNNNNTGC 6 cut(s) 430, 464, 469, 503, 774, 808
BciT130I CCWGG 1 cut(s) 471
BclI TGATCA 1 cut(s) 670
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 1 cut(s) 330
BfmI CTRYAG 3 cut(s) 420, 768, 1007
BfuAI ACCTGC 2 cut(s) 331, 928
BisI GCNGC 3 cut(s) 466, 546, 885
BlsI GCNGC 3 cut(s) 467, 547, 886
Bme1390I CCNGG 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 467
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 1 cut(s) 471
BmrI ACTGGG 1 cut(s) 685
BmsI GCATC 5 cut(s) 28, 308, 450, 495, 532
BmuI ACTGGG 1 cut(s) 685
BpuEI CTTGAG 2 cut(s) 873, 880
BsaJI CCNNGG 2 cut(s) 79, 469
BsaWI WCCGGW 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 4 cut(s) 105, 476, 815, 854
Bse1I ACTGG 1 cut(s) 691
Bse3DI GCAATG 2 cut(s) 18, 618
BseAI TCCGGA 1 cut(s) 444
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 79, 469
BseGI GGATG 4 cut(s) 132, 299, 441, 848
BseLI CCNNNNNNNGG 4 cut(s) 105, 476, 815, 854
BseMI GCAATG 2 cut(s) 18, 618
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 1 cut(s) 691
BseXI GCAGC 2 cut(s) 557, 896
BshFI GGCC 2 cut(s) 197, 468
BshNI GGYRCC 1 cut(s) 11
BsiSI CCGG 1 cut(s) 445
BslFI GGGAC 3 cut(s) 144, 318, 772
BslI CCNNNNNNNGG 4 cut(s) 105, 476, 815, 854
BsmFI GGGAC 3 cut(s) 144, 318, 772
BsmI GAATGC 2 cut(s) 272, 548
BsnI GGCC 2 cut(s) 197, 468
Bsp1286I GDGCHC 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 444
Bsp143I GATC 5 cut(s) 3, 447, 579, 670, 869
Bsp19I CCATGG 1 cut(s) 79
BspACI CCGC 3 cut(s) 289, 465, 719
BspANI GGCC 2 cut(s) 197, 468
BspCNI CTCAG 1 cut(s) 556
BspEI TCCGGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 13
BspMI ACCTGC 2 cut(s) 331, 928
BspPI GGATC 2 cut(s) 455, 587
BspT107I GGYRCC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 18, 618
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 2 cut(s) 79, 469
BssMI GATC 5 cut(s) 3, 447, 579, 670, 869
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 2 cut(s) 118, 284
BstC8I GCNNGC 2 cut(s) 793, 925
BstDEI CTNAG 1 cut(s) 564
BstDSI CCRYGG 1 cut(s) 79
BstF5I GGATG 4 cut(s) 132, 299, 441, 848
BstKTI GATC 5 cut(s) 6, 450, 582, 673, 872
BstMBI GATC 5 cut(s) 3, 447, 579, 670, 869
BstMWI GCNNNNNNNGC 2 cut(s) 194, 492
BstNI CCWGG 1 cut(s) 471
BstNSI RCATGY 4 cut(s) 188, 524, 795, 927
BstSCI CCNGG 1 cut(s) 469
BstSFI CTRYAG 3 cut(s) 420, 768, 1007
BstV1I GCAGC 2 cut(s) 557, 896
BsuRI GGCC 2 cut(s) 197, 468
BtgI CCRYGG 1 cut(s) 79
BtsCI GGATG 4 cut(s) 132, 299, 441, 848
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 2 cut(s) 561, 802
BveI ACCTGC 2 cut(s) 331, 928
Cac8I GCNNGC 2 cut(s) 793, 925
Cfr13I GGNCC 1 cut(s) 467
CseI GACGC 1 cut(s) 69
Csp6I GTAC 1 cut(s) 12
CviJI RGCY 7 cut(s) 70, 146, 197, 468, 495, 887, 894
CviKI_1 RGCY 7 cut(s) 70, 146, 197, 468, 495, 887, 894
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 1 cut(s) 564
DpnI GATC 5 cut(s) 5, 449, 581, 672, 871
DpnII GATC 5 cut(s) 3, 447, 579, 670, 869
DraI TTTAAA 1 cut(s) 705
EaeI YGGCCR 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 944
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 79
FaqI GGGAC 3 cut(s) 144, 318, 772
FauI CCCGC 1 cut(s) 282
FbaI TGATCA 1 cut(s) 670
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 466, 546, 885
FokI GGATG 4 cut(s) 139, 286, 428, 835
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 3 cut(s) 466, 546, 885
FspBI CTAG 1 cut(s) 330
GluI GCNGC 3 cut(s) 466, 546, 885
HaeIII GGCC 2 cut(s) 197, 468
HapII CCGG 1 cut(s) 445
HgaI GACGC 1 cut(s) 69
HincII GTYRAC 2 cut(s) 182, 913
HindII GTYRAC 2 cut(s) 182, 913
HindIII AAGCTT 1 cut(s) 493
HinfI GANTC 4 cut(s) 55, 348, 686, 803
HpaII CCGG 1 cut(s) 445
HphI GGTGA 4 cut(s) 88, 97, 268, 869
Hpy166II GTNNAC 3 cut(s) 88, 182, 913
Hpy188I TCNGA 4 cut(s) 491, 630, 711, 934
Hpy188III TCNNGA 3 cut(s) 35, 445, 451
Hpy8I GTNNAC 3 cut(s) 88, 182, 913
Hpy99I CGWCG 1 cut(s) 63
HpyAV CCTTC 3 cut(s) 98, 220, 809
HpyCH4III ACNGT 2 cut(s) 118, 284
HpyCH4IV ACGT 1 cut(s) 648
HpyCH4V TGCA 9 cut(s) 216, 265, 320, 414, 545, 623, 791, 795, 923
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 492
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 1 cut(s) 648
Kpn2I TCCGGA 1 cut(s) 444
KpnI GGTACC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 5 cut(s) 3, 447, 579, 670, 869
LmnI GCTCC 2 cut(s) 28, 193
Lsp1109I GCAGC 2 cut(s) 557, 896
LweI GCATC 5 cut(s) 28, 308, 450, 495, 532
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 648
MaeIII GTNAC 2 cut(s) 516, 683
MalI GATC 5 cut(s) 5, 449, 581, 672, 871
MboI GATC 5 cut(s) 3, 447, 579, 670, 869
MboII GAAGA 3 cut(s) 426, 842, 953
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 5 cut(s) 220, 430, 660, 666, 944
MlyI GAGTC 1 cut(s) 680
MmeI TCCRAC 1 cut(s) 215
MnlI CCTC 8 cut(s) 104, 370, 563, 570, 590, 622, 974, 986
MroI TCCGGA 1 cut(s) 444
MroXI GAANNNNTTC 1 cut(s) 834
MseI TTAA 3 cut(s) 704, 753, 951
MslI CAYNNNNRTG 4 cut(s) 357, 359, 525, 922
MspI CCGG 1 cut(s) 445
MspR9I CCNGG 1 cut(s) 471
Mva1269I GAATGC 2 cut(s) 272, 548
MvaI CCWGG 1 cut(s) 471
MwoI GCNNNNNNNGC 2 cut(s) 194, 492
NcoI CCATGG 1 cut(s) 79
NdeII GATC 5 cut(s) 3, 447, 579, 670, 869
NlaIV GGNNCC 1 cut(s) 13
NmuCI GTSAC 2 cut(s) 516, 683
NspI RCATGY 4 cut(s) 188, 524, 795, 927
OliI CACNNNNGTG 1 cut(s) 357
PaeI GCATGC 2 cut(s) 795, 927
PaqCI CACCTGC 2 cut(s) 331, 928
PciI ACATGT 1 cut(s) 520
PctI GAATGC 2 cut(s) 272, 548
PdmI GAANNNNTTC 1 cut(s) 834
PfeI GAWTC 3 cut(s) 55, 348, 803
PflMI CCANNNNNTGG 1 cut(s) 854
PkrI GCNGC 3 cut(s) 467, 547, 886
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
PscI ACATGT 1 cut(s) 520
PsiI TTATAA 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 1 cut(s) 467
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 4 cut(s) 357, 359, 525, 922
SalI GTCGAC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 704, 753, 951
SatI GCNGC 3 cut(s) 466, 546, 885
Sau3AI GATC 5 cut(s) 3, 447, 579, 670, 869
Sau96I GGNCC 1 cut(s) 467
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 5 cut(s) 28, 308, 450, 495, 532
SfcI CTRYAG 3 cut(s) 420, 768, 1007
SmiMI CAYNNNNRTG 4 cut(s) 357, 359, 525, 922
SmlI CTYRAG 2 cut(s) 888, 895
SmoI CTYRAG 2 cut(s) 888, 895
SpeI ACTAGT 1 cut(s) 329
SphI GCATGC 2 cut(s) 795, 927
Sse9I AATT 5 cut(s) 220, 430, 660, 666, 944
SsiI CCGC 3 cut(s) 289, 465, 719
SspI AATATT 3 cut(s) 238, 457, 677
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 1 cut(s) 469
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 2 cut(s) 118, 284
TaiI ACGT 1 cut(s) 651
TaqI TCGA 3 cut(s) 58, 120, 181
TasI AATT 5 cut(s) 220, 430, 660, 666, 944
TauI GCSGC 1 cut(s) 468
TfiI GAWTC 3 cut(s) 55, 348, 803
Tru1I TTAA 3 cut(s) 704, 753, 951
Tru9I TTAA 3 cut(s) 704, 753, 951
TscAI CASTG 2 cut(s) 568, 802
TseFI GTSAC 2 cut(s) 516, 683
TseI GCWGC 2 cut(s) 545, 884
Tsp45I GTSAC 2 cut(s) 516, 683
TspDTI ATGAA 1 cut(s) 937
TspRI CASTG 2 cut(s) 568, 802
Van91I CCANNNNNTGG 1 cut(s) 854
XapI RAATTY 2 cut(s) 220, 944
XceI RCATGY 4 cut(s) 188, 524, 795, 927
XmiI GTMKAC 1 cut(s) 181
XmnI GAANNNNTTC 1 cut(s) 834
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.