Rw2G031180

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009481
Physical Location & Seq
Reverse (-)
51286943 .. 51290207
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031180.1

Sequence Viewer

Length: 1380 bp
ATGAAAACCCTTCGATTCAGCTCATCTTCTTCTTCTTCTTCCACTTGTCTCATTCTTCTCCTTCATATATTCTCTCTCTTTCTGAGCTCAAATACTACTCCTACTACTGATAGAGAGAAATTTGACTCTCTCTTACGGCTCCCCCAATCCGGGTCTGCCAGAACCCGACATAGATCCAAGAGGGTTCTCTTTGTTGGCGATTTTGGTGCCAAAGGAGATGGCATCACTGATGATTCTCAGGCTTTCAAAAATGCTTGGGAGGTTGCTTGTTCTATTCCCACACGGACAAGAATTCTAATTCCCTCCGGAAGGACTTACCTTATCCATCCTGTTGATATTGCTGGACCGTGTCGATCAAAGGTGACTTTAATGATCTCTGGTACCATCATTGCTCCCATCAATCCTGATGCTTGGATTGGTTTGAATCGACGCAAGTGGCTTTATTTCCATGGGGTGAACCATCTCACCATTGAAGGGGGAGGAACAGTCGATGGGATGGGACAGGAATGGTGGGCTCGTTCTTGTAAGACCAACTCAACAAATCCATGTCGACATGCTCCAACGGCCATTACATTCCATAGGTGCAAGAATTTGAAGGTCAAAAATATTATGATTGTCAATAGTCAAAAAATGCACATGGCATTCACCAACTGTATGCGGGTATTGGCATCCCATCTCCAAGTTATTGCACCTGCCACTAGTCCCAATACTGATGGAATCCACATTAGTGTGTCAAAGGGTGTTGAGGTCAAGGATAGCATAATAAGAACAGGAGATGACTGCATTTCTATAGTTAGCAATTCTTCACGCATCCGGATCGTGAATATTGTCTGCGGCCCTGGTCATGGCATAAGCATCGGAAGCTTGGGAAAATCAAACTCTTGGTCACATGTGCGTGATGTAGTGGTTGATGCAGCATTCCTCTCTAACACTGAGAATGGAGTGAGGATCAAAACATGGCAGGGAGGTAGTGGTTTTGCCTCTAACATTGCATTTCGGAATGTGGTCATGGACAACGTATCAAATCCAATTATAATTGATCAATATTATTGTGACTCCCAGTTGCCATGTTTAAATCAGACTTTGGCGGTCAAAGTTGAGAATATATCCTTTATACACATTAAAGGGACTTCTGCTACAGACGAAGCAATACGATTTGCATGCAGTGATGATTCTCCATGTGAAGGTTTATATTTAGAAGATGTTCAACTTCTACCATCCAATGGTGAAATAACAAGATCATTTTGTTGGGCAGCTCAAGGCTCAAGTTTGGGTTCAGTTGACCCACCTGCATGCTTTTCCGATAGTGAAGAATTCATTAAACAAAAAGTCCTCATACACCCTGTAAGTTTGGAATATGGAGGAAAGGCAATCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

459

Amino Acids

50.09

Weight (kDa)

8.77

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 90 - 406 4.6e-96 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1034
AarI CACCTGC 2 cut(s) 700, 1297
Acc36I ACCTGC 2 cut(s) 700, 1297
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 2 cut(s) 206, 380
AccB7I CCANNNNNTGG 1 cut(s) 1223
AccI GTMKAC 1 cut(s) 550
AccIII TCCGGA 2 cut(s) 305, 813
AciI CCGC 3 cut(s) 658, 834, 1088
AclWI GGATC 3 cut(s) 168, 824, 956
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 4 cut(s) 119, 291, 589, 1313
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 7 cut(s) 149, 150, 309, 474, 845, 1184, 1223
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 5 cut(s) 247, 424, 473, 595, 1208
AhlI ACTAGT 1 cut(s) 698
AjnI CCWGG 1 cut(s) 838
AleI CACNNNNGTG 1 cut(s) 726
AluBI AGCT 4 cut(s) 21, 87, 864, 1256
AluI AGCT 4 cut(s) 21, 87, 864, 1256
Alw21I GWGCWC 1 cut(s) 89
Alw26I GTCTC 1 cut(s) 53
AlwI GGATC 3 cut(s) 168, 824, 956
Aor13HI TCCGGA 2 cut(s) 305, 813
AoxI GGCC 2 cut(s) 564, 835
ApeKI GCWGC 2 cut(s) 914, 1253
ApoI RAATTY 4 cut(s) 119, 291, 589, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 869, 901
Asp700I GAANNNNTTC 1 cut(s) 1203
Asp718I GGTACC 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 344, 836
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 5 cut(s) 373, 457, 466, 637, 1238
AvaII GGWCC 1 cut(s) 344
BanI GGYRCC 2 cut(s) 206, 380
BanII GRGCYC 2 cut(s) 89, 517
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 926, 1265
BceAI ACGGC 2 cut(s) 152, 579
BcgI CGANNNNNNTGC 8 cut(s) 188, 222, 799, 833, 838, 872, 1143, 1177
BciT130I CCWGG 1 cut(s) 840
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 53
BcuI ACTAGT 1 cut(s) 698
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 3 cut(s) 789, 1137, 1376
BfuAI ACCTGC 2 cut(s) 700, 1297
BisI GCNGC 3 cut(s) 835, 915, 1254
BlsI GCNGC 3 cut(s) 836, 916, 1255
Bme1390I CCNGG 2 cut(s) 151, 840
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 2 cut(s) 344, 836
BmiI GGNNCC 3 cut(s) 140, 208, 382
BmrFI CCNGG 2 cut(s) 151, 840
BmrI ACTGGG 1 cut(s) 1054
BmsI GCATC 6 cut(s) 231, 397, 677, 819, 864, 901
BmuI ACTGGG 1 cut(s) 1054
BpuEI CTTGAG 2 cut(s) 1242, 1249
BpuMI CCSGG 1 cut(s) 151
BsaJI CCNNGG 2 cut(s) 448, 838
BsaWI WCCGGW 2 cut(s) 305, 813
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 150, 309, 474, 845, 1184, 1223
Bse1I ACTGG 1 cut(s) 1060
Bse3DI GCAATG 2 cut(s) 387, 987
BseAI TCCGGA 2 cut(s) 305, 813
BseBI CCWGG 1 cut(s) 840
BseDI CCNNGG 2 cut(s) 448, 838
BseGI GGATG 5 cut(s) 325, 501, 668, 810, 1217
BseLI CCNNNNNNNGG 7 cut(s) 149, 150, 309, 474, 845, 1184, 1223
BseMI GCAATG 2 cut(s) 387, 987
BseMII CTCAG 3 cut(s) 74, 251, 924
BseNI ACTGG 1 cut(s) 1060
BseXI GCAGC 2 cut(s) 926, 1265
BshFI GGCC 2 cut(s) 566, 837
BshNI GGYRCC 2 cut(s) 206, 380
BsiHKAI GWGCWC 1 cut(s) 89
BsiSI CCGG 3 cut(s) 150, 306, 814
BslFI GGGAC 3 cut(s) 513, 687, 1141
BslI CCNNNNNNNGG 7 cut(s) 149, 150, 309, 474, 845, 1184, 1223
BsmAI GTCTC 1 cut(s) 53
BsmFI GGGAC 3 cut(s) 513, 687, 1141
BsmI GAATGC 2 cut(s) 641, 917
BsnI GGCC 2 cut(s) 566, 837
Bsp1286I GDGCHC 2 cut(s) 89, 517
Bsp13I TCCGGA 2 cut(s) 305, 813
Bsp143I GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 3 cut(s) 658, 834, 1088
BspANI GGCC 2 cut(s) 566, 837
BspCNI CTCAG 3 cut(s) 75, 250, 925
BspEI TCCGGA 2 cut(s) 305, 813
BspLI GGNNCC 3 cut(s) 140, 208, 382
BspMI ACCTGC 2 cut(s) 700, 1297
BspPI GGATC 3 cut(s) 168, 824, 956
BspT107I GGYRCC 2 cut(s) 206, 380
BsrDI GCAATG 2 cut(s) 387, 987
BsrI ACTGG 1 cut(s) 1060
BssECI CCNNGG 2 cut(s) 448, 838
BssMI GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
BssT1I CCWWGG 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 3 cut(s) 348, 487, 653
BstC8I GCNNGC 2 cut(s) 1162, 1294
BstDEI CTNAG 3 cut(s) 83, 237, 933
BstDSI CCRYGG 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 307
BstF5I GGATG 5 cut(s) 325, 501, 668, 810, 1217
BstKTI GATC 7 cut(s) 176, 356, 375, 819, 951, 1042, 1241
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
BstMWI GCNNNNNNNGC 2 cut(s) 563, 861
BstNI CCWGG 1 cut(s) 840
BstNSI RCATGY 4 cut(s) 557, 893, 1164, 1296
BstSCI CCNGG 2 cut(s) 149, 838
BstSFI CTRYAG 3 cut(s) 789, 1137, 1376
BstV1I GCAGC 2 cut(s) 926, 1265
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuRI GGCC 2 cut(s) 566, 837
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 5 cut(s) 325, 501, 668, 810, 1217
BtsI GCAGTG 1 cut(s) 1171
BtsIMutI CAGTG 3 cut(s) 225, 930, 1171
BveI ACCTGC 2 cut(s) 700, 1297
Cac8I GCNNGC 2 cut(s) 1162, 1294
Cfr13I GGNCC 2 cut(s) 344, 836
CseI GACGC 1 cut(s) 438
Csp6I GTAC 1 cut(s) 381
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 3 cut(s) 83, 237, 933
DpnI GATC 7 cut(s) 175, 355, 374, 818, 950, 1041, 1240
DpnII GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
DraI TTTAAA 1 cut(s) 1074
EaeI YGGCCR 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 448
Eco24I GRGCYC 2 cut(s) 89, 517
Eco47I GGWCC 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 87
EcoICRI GAGCTC 1 cut(s) 87
EcoNI CCTNNNNNAGG 1 cut(s) 307
EcoRI GAATTC 2 cut(s) 291, 1313
EcoRII CCWGG 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 448
EcoT38I GRGCYC 2 cut(s) 89, 517
ErhI CCWWGG 1 cut(s) 448
FaqI GGGAC 3 cut(s) 513, 687, 1141
FauI CCCGC 1 cut(s) 651
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 3 cut(s) 835, 915, 1254
FokI GGATG 5 cut(s) 312, 508, 655, 797, 1204
FriOI GRGCYC 2 cut(s) 89, 517
Fsp4HI GCNGC 3 cut(s) 835, 915, 1254
FspBI CTAG 1 cut(s) 699
GluI GCNGC 3 cut(s) 835, 915, 1254
HaeIII GGCC 2 cut(s) 566, 837
HapII CCGG 3 cut(s) 150, 306, 814
HgaI GACGC 1 cut(s) 438
HincII GTYRAC 2 cut(s) 551, 1282
HindII GTYRAC 2 cut(s) 551, 1282
HindIII AAGCTT 1 cut(s) 862
HinfI GANTC 7 cut(s) 15, 125, 233, 424, 717, 1055, 1172
HpaII CCGG 3 cut(s) 150, 306, 814
HphI GGTGA 5 cut(s) 373, 457, 466, 637, 1238
Hpy166II GTNNAC 3 cut(s) 457, 551, 1282
Hpy188I TCNGA 5 cut(s) 84, 860, 999, 1080, 1303
Hpy188III TCNNGA 4 cut(s) 306, 404, 814, 820
Hpy8I GTNNAC 3 cut(s) 457, 551, 1282
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 6 cut(s) 20, 71, 303, 467, 589, 1178
HpyCH4III ACNGT 3 cut(s) 348, 487, 653
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 9 cut(s) 585, 634, 689, 783, 914, 992, 1160, 1164, 1292
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 861
HpyF3I CTNAG 3 cut(s) 83, 237, 933
HpySE526I ACGT 1 cut(s) 1017
Kpn2I TCCGGA 2 cut(s) 305, 813
KpnI GGTACC 1 cut(s) 384
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
LmnI GCTCC 3 cut(s) 144, 397, 562
Lsp1109I GCAGC 2 cut(s) 926, 1265
LweI GCATC 6 cut(s) 231, 397, 677, 819, 864, 901
MaeI CTAG 1 cut(s) 699
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 3 cut(s) 361, 885, 1052
MalI GATC 7 cut(s) 175, 355, 374, 818, 950, 1041, 1240
MboI GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
MboII GAAGA 9 cut(s) 18, 21, 24, 27, 30, 47, 795, 1211, 1322
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 2 cut(s) 89, 517
MluCI AATT 8 cut(s) 119, 291, 297, 589, 799, 1029, 1035, 1313
MlyI GAGTC 2 cut(s) 119, 1049
MmeI TCCRAC 1 cut(s) 584
MroI TCCGGA 2 cut(s) 305, 813
MroXI GAANNNNTTC 1 cut(s) 1203
MseI TTAA 4 cut(s) 368, 1073, 1122, 1320
MslI CAYNNNNRTG 4 cut(s) 726, 728, 894, 1291
MspI CCGG 3 cut(s) 150, 306, 814
MspR9I CCNGG 2 cut(s) 151, 840
Mva1269I GAATGC 2 cut(s) 641, 917
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 2 cut(s) 563, 861
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 448
NdeII GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
NlaIV GGNNCC 3 cut(s) 140, 208, 382
NmuCI GTSAC 3 cut(s) 361, 885, 1052
NspI RCATGY 4 cut(s) 557, 893, 1164, 1296
OliI CACNNNNGTG 1 cut(s) 726
PaeI GCATGC 2 cut(s) 1164, 1296
PaqCI CACCTGC 2 cut(s) 700, 1297
PciI ACATGT 1 cut(s) 889
PctI GAATGC 2 cut(s) 641, 917
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 5 cut(s) 15, 233, 424, 717, 1172
PflFI GACNNNGTC 1 cut(s) 348
PflMI CCANNNNNTGG 1 cut(s) 1223
PkrI GCNGC 3 cut(s) 836, 916, 1255
PleI GAGTC 2 cut(s) 119, 1049
PpsI GAGTC 2 cut(s) 119, 1049
PscI ACATGT 1 cut(s) 889
PsiI TTATAA 1 cut(s) 1034
Psp124BI GAGCTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 838
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 3 cut(s) 140, 208, 382
PspPI GGNCC 2 cut(s) 344, 836
PsuI RGATCY 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 348
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 4 cut(s) 726, 728, 894, 1291
SacI GAGCTC 1 cut(s) 89
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 4 cut(s) 368, 1073, 1122, 1320
SatI GCNGC 3 cut(s) 835, 915, 1254
Sau3AI GATC 7 cut(s) 173, 353, 372, 816, 948, 1039, 1238
Sau96I GGNCC 2 cut(s) 344, 836
SchI GAGTC 2 cut(s) 119, 1049
ScrFI CCNGG 2 cut(s) 151, 840
SduI GDGCHC 2 cut(s) 89, 517
SfaNI GCATC 6 cut(s) 231, 397, 677, 819, 864, 901
SfcI CTRYAG 3 cut(s) 789, 1137, 1376
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 4 cut(s) 726, 728, 894, 1291
SmlI CTYRAG 2 cut(s) 1257, 1264
SmoI CTYRAG 2 cut(s) 1257, 1264
SpeI ACTAGT 1 cut(s) 698
SphI GCATGC 2 cut(s) 1164, 1296
Sse9I AATT 8 cut(s) 119, 291, 297, 589, 799, 1029, 1035, 1313
SsiI CCGC 3 cut(s) 658, 834, 1088
SspI AATATT 3 cut(s) 607, 826, 1046
SspMI CTAG 1 cut(s) 699
SstI GAGCTC 1 cut(s) 89
StyD4I CCNGG 2 cut(s) 149, 838
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 3 cut(s) 348, 487, 653
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 5 cut(s) 13, 352, 427, 489, 550
TasI AATT 8 cut(s) 119, 291, 297, 589, 799, 1029, 1035, 1313
TauI GCSGC 1 cut(s) 837
TfiI GAWTC 5 cut(s) 15, 233, 424, 717, 1172
Tru1I TTAA 4 cut(s) 368, 1073, 1122, 1320
Tru9I TTAA 4 cut(s) 368, 1073, 1122, 1320
TscAI CASTG 3 cut(s) 232, 937, 1171
TseFI GTSAC 3 cut(s) 361, 885, 1052
TseI GCWGC 2 cut(s) 914, 1253
Tsp45I GTSAC 3 cut(s) 361, 885, 1052
TspDTI ATGAA 3 cut(s) 17, 53, 1306
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 3 cut(s) 232, 937, 1171
Tth111I GACNNNGTC 1 cut(s) 348
Van91I CCANNNNNTGG 1 cut(s) 1223
VpaK11BI GGWCC 1 cut(s) 344
XagI CCTNNNNNAGG 1 cut(s) 307
XapI RAATTY 4 cut(s) 119, 291, 589, 1313
XceI RCATGY 4 cut(s) 557, 893, 1164, 1296
XmiI GTMKAC 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.