Rh2AG381400

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009481
Physical Location & Seq
Reverse (-)
57197182 .. 57200455
3274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG381400.1

Sequence Viewer

Length: 1386 bp
ATGAAAACCCTTCGATTCTACTCATCTTCTTCTTCTTCTTCTTCTTCCACTTGTCTCATTCTTCTCCTTCATATATTCTCTCTCTTTCTGAGCTCAAATACTACTCCTACTACTGATAGAGAGAAATTTGACTCTCTCTTACGGCTCCCCCAATCCGGGTCTACCAGAACCCGACATAGATCCAAGAGGGTTCTCTTTGTTGGCGATTTTGGTGCCAAAGGAGATGGCATCACTGATGATTCTAAGGCTTTCAAAAATGCTTGGGAGGTTGCTTGTTCTATTCCCACACGGACAAGAATTCTAATTCCCTCCGGAAGGACTTACCTTATCCATCCTGTTGATATTGCTGGACCGTGTCGATCAAAGGTGACTTTAATGATCTCTGGTACCATCATTGCTCCCATCAATCCTGATGCTTGGATTGGTTTGAATCGACGCAAGTGGCTTTATTTCCATGGGGTGAACCATCTCACCATTGAAGGGGGAGGAACAGTCGATGGGATGGGACAGGAATGGTGGGCTCGTTCTTGTAAGACCAACTCAACAAATCCATGTCGACATGCTCCAACGGCCATTACATTCCATAGGTGCAAGAATTTGAAGGTCAAAAATATTATGATTGTCAATAGTCAAAAAATGCACGTGGCATTCACCAACTGTATGCGGGTATTAGCATCCCATCTCCAAGTTATTGCACCTGCCGCTAGTCCCAATACTGATGGAATCCACATTAGTGTGTCAAAGGGTGTTGAGGTCAAGGATAGCATAATAAGAACAGGAGATGACTGCATTTCTATAGTTAGCAATTCTTCACGCATCCGGATCGTGAATATTGTCTGCGGCCCTGGTCATGGCATAAGCATCGGAAGCTTGGGAAAATCAAACTCTTGGTCACATGTGCGTGATGTAGTGGTTGATGCAGCATTCCTCTCTAACACTGAGAATGGAGTGAGGATCAAAACATGGCAGGGAGGTAGTGGTTTTGCCTCTAACATTGCATTTCGGAATGTCGTCATGGACAACGTATCAAATCCAATTATAATTGATCAATATTATTGTGACTCCCAGTTGCCATGTTTAAATCAGACTTTGGCGGTCAAAGTTGAGAATATATCCTTTATACACATTAAAGGGACTTCTGCTACAGACGAAGCAATACGATTTGCATGCAGTGATGATTCTCCATGTGAAGGTTTATATTTAGAAGATGTTCAACTTCTACCATCCAATGGTGAAATAACAAGATCATTTTGTTGGGCAGCTCAAGGCTCAAGTTTGGGTTCAGTTGACCCACCTGCATGCTTTTCCGATAGTGAAGAATTCATTAAACAAAAAGTCCTCATACACCCTGTAAGTTTGGAATATGGAGGAAAGGCAATCCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

461

Amino Acids

50.31

Weight (kDa)

8.84

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 92 - 408 1.3e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1040
AarI CACCTGC 2 cut(s) 706, 1303
Acc36I ACCTGC 2 cut(s) 706, 1303
Acc65I GGTACC 1 cut(s) 386
AccB1I GGYRCC 2 cut(s) 212, 386
AccB7I CCANNNNNTGG 1 cut(s) 1229
AccI GTMKAC 2 cut(s) 161, 556
AccIII TCCGGA 2 cut(s) 311, 819
AciI CCGC 4 cut(s) 664, 702, 840, 1094
AclWI GGATC 3 cut(s) 174, 830, 962
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 4 cut(s) 125, 297, 595, 1319
AcvI CACGTG 1 cut(s) 643
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 7 cut(s) 155, 156, 315, 480, 851, 1190, 1229
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 5 cut(s) 253, 430, 479, 601, 1214
AjnI CCWGG 1 cut(s) 844
AleI CACNNNNGTG 1 cut(s) 732
AluBI AGCT 3 cut(s) 93, 870, 1262
AluI AGCT 3 cut(s) 93, 870, 1262
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 3 cut(s) 174, 830, 962
Aor13HI TCCGGA 2 cut(s) 311, 819
AoxI GGCC 2 cut(s) 570, 841
ApeKI GCWGC 2 cut(s) 920, 1259
ApoI RAATTY 4 cut(s) 125, 297, 595, 1319
ArsI GACNNNNNNTTYG 2 cut(s) 875, 907
Asp700I GAANNNNTTC 1 cut(s) 1209
Asp718I GGTACC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 350, 842
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 5 cut(s) 379, 463, 472, 643, 1244
AvaII GGWCC 1 cut(s) 350
BanI GGYRCC 2 cut(s) 212, 386
BanII GRGCYC 2 cut(s) 95, 523
BbrPI CACGTG 1 cut(s) 643
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 2 cut(s) 932, 1271
BceAI ACGGC 2 cut(s) 158, 585
BcgI CGANNNNNNTGC 8 cut(s) 194, 228, 805, 839, 844, 878, 1149, 1183
BciT130I CCWGG 1 cut(s) 846
BclI TGATCA 1 cut(s) 1045
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 1 cut(s) 705
BfmI CTRYAG 3 cut(s) 795, 1143, 1382
BfuAI ACCTGC 2 cut(s) 706, 1303
BisI GCNGC 4 cut(s) 702, 841, 921, 1260
BlsI GCNGC 4 cut(s) 703, 842, 922, 1261
Bme1390I CCNGG 2 cut(s) 157, 846
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 350, 842
BmiI GGNNCC 3 cut(s) 146, 214, 388
BmrFI CCNGG 2 cut(s) 157, 846
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 6 cut(s) 237, 403, 683, 825, 870, 907
BmuI ACTGGG 1 cut(s) 1060
BpuEI CTTGAG 2 cut(s) 1248, 1255
BpuMI CCSGG 1 cut(s) 157
BsaAI YACGTR 1 cut(s) 643
BsaJI CCNNGG 2 cut(s) 454, 844
BsaWI WCCGGW 2 cut(s) 311, 819
BsaXI ACNNNNNCTCC 2 cut(s) 477, 507
Bsc4I CCNNNNNNNGG 7 cut(s) 155, 156, 315, 480, 851, 1190, 1229
Bse1I ACTGG 1 cut(s) 1066
Bse3DI GCAATG 2 cut(s) 393, 993
BseAI TCCGGA 2 cut(s) 311, 819
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 454, 844
BseGI GGATG 5 cut(s) 331, 507, 674, 816, 1223
BseLI CCNNNNNNNGG 7 cut(s) 155, 156, 315, 480, 851, 1190, 1229
BseMI GCAATG 2 cut(s) 393, 993
BseMII CTCAG 2 cut(s) 80, 930
BseNI ACTGG 1 cut(s) 1066
BseXI GCAGC 2 cut(s) 932, 1271
BshFI GGCC 2 cut(s) 572, 843
BshNI GGYRCC 2 cut(s) 212, 386
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 156, 312, 820
BslFI GGGAC 3 cut(s) 519, 693, 1147
BslI CCNNNNNNNGG 7 cut(s) 155, 156, 315, 480, 851, 1190, 1229
BsmAI GTCTC 1 cut(s) 59
BsmFI GGGAC 3 cut(s) 519, 693, 1147
BsmI GAATGC 2 cut(s) 647, 923
BsnI GGCC 2 cut(s) 572, 843
Bsp1286I GDGCHC 2 cut(s) 95, 523
Bsp13I TCCGGA 2 cut(s) 311, 819
Bsp143I GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 4 cut(s) 664, 702, 840, 1094
BspANI GGCC 2 cut(s) 572, 843
BspCNI CTCAG 2 cut(s) 81, 931
BspEI TCCGGA 2 cut(s) 311, 819
BspLI GGNNCC 3 cut(s) 146, 214, 388
BspMI ACCTGC 2 cut(s) 706, 1303
BspPI GGATC 3 cut(s) 174, 830, 962
BspT107I GGYRCC 2 cut(s) 212, 386
BsrDI GCAATG 2 cut(s) 393, 993
BsrI ACTGG 1 cut(s) 1066
BssECI CCNNGG 2 cut(s) 454, 844
BssMI GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
BssT1I CCWWGG 1 cut(s) 454
Bst2UI CCWGG 1 cut(s) 846
Bst4CI ACNGT 3 cut(s) 354, 493, 659
BstBAI YACGTR 1 cut(s) 643
BstC8I GCNNGC 2 cut(s) 1168, 1300
BstDEI CTNAG 3 cut(s) 89, 243, 939
BstDSI CCRYGG 1 cut(s) 454
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 5 cut(s) 331, 507, 674, 816, 1223
BstKTI GATC 7 cut(s) 182, 362, 381, 825, 957, 1048, 1247
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
BstMWI GCNNNNNNNGC 3 cut(s) 569, 701, 867
BstNI CCWGG 1 cut(s) 846
BstNSI RCATGY 4 cut(s) 563, 899, 1170, 1302
BstSCI CCNGG 2 cut(s) 155, 844
BstSFI CTRYAG 3 cut(s) 795, 1143, 1382
BstV1I GCAGC 2 cut(s) 932, 1271
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 2 cut(s) 572, 843
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 5 cut(s) 331, 507, 674, 816, 1223
BtsI GCAGTG 1 cut(s) 1177
BtsIMutI CAGTG 3 cut(s) 231, 936, 1177
BveI ACCTGC 2 cut(s) 706, 1303
Cac8I GCNNGC 2 cut(s) 1168, 1300
Cfr13I GGNCC 2 cut(s) 350, 842
CseI GACGC 1 cut(s) 444
Csp6I GTAC 1 cut(s) 387
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 3 cut(s) 89, 243, 939
DpnI GATC 7 cut(s) 181, 361, 380, 824, 956, 1047, 1246
DpnII GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
DraI TTTAAA 1 cut(s) 1080
EaeI YGGCCR 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 2 cut(s) 95, 523
Eco47I GGWCC 1 cut(s) 350
Eco53kI GAGCTC 1 cut(s) 93
Eco72I CACGTG 1 cut(s) 643
EcoICRI GAGCTC 1 cut(s) 93
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoRI GAATTC 2 cut(s) 297, 1319
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 2 cut(s) 95, 523
ErhI CCWWGG 1 cut(s) 454
FaqI GGGAC 3 cut(s) 519, 693, 1147
FauI CCCGC 1 cut(s) 657
FbaI TGATCA 1 cut(s) 1045
FblI GTMKAC 2 cut(s) 161, 556
Fnu4HI GCNGC 4 cut(s) 702, 841, 921, 1260
FokI GGATG 5 cut(s) 318, 514, 661, 803, 1210
FriOI GRGCYC 2 cut(s) 95, 523
Fsp4HI GCNGC 4 cut(s) 702, 841, 921, 1260
FspBI CTAG 1 cut(s) 705
GluI GCNGC 4 cut(s) 702, 841, 921, 1260
HaeIII GGCC 2 cut(s) 572, 843
HapII CCGG 3 cut(s) 156, 312, 820
HgaI GACGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 557, 1288
HindII GTYRAC 2 cut(s) 557, 1288
HindIII AAGCTT 1 cut(s) 868
HinfI GANTC 7 cut(s) 15, 131, 239, 430, 723, 1061, 1178
HpaII CCGG 3 cut(s) 156, 312, 820
HphI GGTGA 5 cut(s) 379, 463, 472, 643, 1244
Hpy166II GTNNAC 4 cut(s) 162, 463, 557, 1288
Hpy188I TCNGA 5 cut(s) 90, 866, 1005, 1086, 1309
Hpy188III TCNNGA 4 cut(s) 312, 410, 820, 826
Hpy8I GTNNAC 4 cut(s) 162, 463, 557, 1288
Hpy99I CGWCG 1 cut(s) 438
HpyAV CCTTC 6 cut(s) 20, 77, 309, 473, 595, 1184
HpyCH4III ACNGT 3 cut(s) 354, 493, 659
HpyCH4IV ACGT 2 cut(s) 642, 1023
HpyCH4V TGCA 9 cut(s) 591, 640, 695, 789, 920, 998, 1166, 1170, 1298
HpyF10VI GCNNNNNNNGC 3 cut(s) 569, 701, 867
HpyF3I CTNAG 3 cut(s) 89, 243, 939
HpySE526I ACGT 2 cut(s) 642, 1023
Kpn2I TCCGGA 2 cut(s) 311, 819
KpnI GGTACC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 1045
Kzo9I GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
LmnI GCTCC 3 cut(s) 150, 403, 568
Lsp1109I GCAGC 2 cut(s) 932, 1271
LweI GCATC 6 cut(s) 237, 403, 683, 825, 870, 907
MaeI CTAG 1 cut(s) 705
MaeII ACGT 2 cut(s) 642, 1023
MaeIII GTNAC 3 cut(s) 367, 891, 1058
MalI GATC 7 cut(s) 181, 361, 380, 824, 956, 1047, 1246
MboI GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 2 cut(s) 95, 523
MluCI AATT 8 cut(s) 125, 297, 303, 595, 805, 1035, 1041, 1319
MlyI GAGTC 2 cut(s) 125, 1055
MmeI TCCRAC 1 cut(s) 590
MroI TCCGGA 2 cut(s) 311, 819
MroXI GAANNNNTTC 1 cut(s) 1209
MseI TTAA 4 cut(s) 374, 1079, 1128, 1326
MslI CAYNNNNRTG 4 cut(s) 732, 734, 900, 1297
MspI CCGG 3 cut(s) 156, 312, 820
MspR9I CCNGG 2 cut(s) 157, 846
Mva1269I GAATGC 2 cut(s) 647, 923
MvaI CCWGG 1 cut(s) 846
MwoI GCNNNNNNNGC 3 cut(s) 569, 701, 867
NciI CCSGG 1 cut(s) 157
NcoI CCATGG 1 cut(s) 454
NdeII GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
NlaIV GGNNCC 3 cut(s) 146, 214, 388
NmuCI GTSAC 3 cut(s) 367, 891, 1058
NspI RCATGY 4 cut(s) 563, 899, 1170, 1302
OliI CACNNNNGTG 1 cut(s) 732
PaeI GCATGC 2 cut(s) 1170, 1302
PaqCI CACCTGC 2 cut(s) 706, 1303
PciI ACATGT 1 cut(s) 895
PctI GAATGC 2 cut(s) 647, 923
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 5 cut(s) 15, 239, 430, 723, 1178
PflFI GACNNNGTC 1 cut(s) 354
PflMI CCANNNNNTGG 1 cut(s) 1229
PkrI GCNGC 4 cut(s) 703, 842, 922, 1261
PleI GAGTC 2 cut(s) 125, 1055
PmaCI CACGTG 1 cut(s) 643
PmlI CACGTG 1 cut(s) 643
PpsI GAGTC 2 cut(s) 125, 1055
Ppu21I YACGTR 1 cut(s) 643
PscI ACATGT 1 cut(s) 895
PsiI TTATAA 1 cut(s) 1040
Psp124BI GAGCTC 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 844
PspCI CACGTG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 3 cut(s) 146, 214, 388
PspPI GGNCC 2 cut(s) 350, 842
PsuI RGATCY 1 cut(s) 179
PsyI GACNNNGTC 1 cut(s) 354
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
RseI CAYNNNNRTG 4 cut(s) 732, 734, 900, 1297
SacI GAGCTC 1 cut(s) 95
SalI GTCGAC 1 cut(s) 555
SaqAI TTAA 4 cut(s) 374, 1079, 1128, 1326
SatI GCNGC 4 cut(s) 702, 841, 921, 1260
Sau3AI GATC 7 cut(s) 179, 359, 378, 822, 954, 1045, 1244
Sau96I GGNCC 2 cut(s) 350, 842
SchI GAGTC 2 cut(s) 125, 1055
ScrFI CCNGG 2 cut(s) 157, 846
SduI GDGCHC 2 cut(s) 95, 523
SfaNI GCATC 6 cut(s) 237, 403, 683, 825, 870, 907
SfcI CTRYAG 3 cut(s) 795, 1143, 1382
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 4 cut(s) 732, 734, 900, 1297
SmlI CTYRAG 2 cut(s) 1263, 1270
SmoI CTYRAG 2 cut(s) 1263, 1270
SphI GCATGC 2 cut(s) 1170, 1302
Sse9I AATT 8 cut(s) 125, 297, 303, 595, 805, 1035, 1041, 1319
SsiI CCGC 4 cut(s) 664, 702, 840, 1094
SspI AATATT 3 cut(s) 613, 832, 1052
SspMI CTAG 1 cut(s) 705
SstI GAGCTC 1 cut(s) 95
StyD4I CCNGG 2 cut(s) 155, 844
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 3 cut(s) 354, 493, 659
TaiI ACGT 2 cut(s) 645, 1026
TaqI TCGA 5 cut(s) 13, 358, 433, 495, 556
TasI AATT 8 cut(s) 125, 297, 303, 595, 805, 1035, 1041, 1319
TauI GCSGC 2 cut(s) 704, 843
TfiI GAWTC 5 cut(s) 15, 239, 430, 723, 1178
Tru1I TTAA 4 cut(s) 374, 1079, 1128, 1326
Tru9I TTAA 4 cut(s) 374, 1079, 1128, 1326
TscAI CASTG 3 cut(s) 238, 943, 1177
TseFI GTSAC 3 cut(s) 367, 891, 1058
TseI GCWGC 2 cut(s) 920, 1259
Tsp45I GTSAC 3 cut(s) 367, 891, 1058
TspDTI ATGAA 3 cut(s) 17, 59, 1312
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 3 cut(s) 238, 943, 1177
Tth111I GACNNNGTC 1 cut(s) 354
Van91I CCANNNNNTGG 1 cut(s) 1229
VpaK11BI GGWCC 1 cut(s) 350
XagI CCTNNNNNAGG 1 cut(s) 313
XapI RAATTY 4 cut(s) 125, 297, 595, 1319
XceI RCATGY 4 cut(s) 563, 899, 1170, 1302
XmiI GTMKAC 2 cut(s) 161, 556
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.