Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005622

Physical Location & Seq

Genomic Coordinates Reverse (-)

88320 .. 94753

Gene Structure 6434 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G01130.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3342 bp

ATGACGAATCGTCTTCCTCCTTCGCTGTACGTTCCGCCTCACCAGCGCCTTGGTTCCGATTACGGCTTCAATCCTGTTCCTCTCTCTCCAGTTCGCTATGTCTCCGCTTATGATGACCGCGTTTCCGAGGATCGCCAACCGCAGGAGGGTACCTTTCACTGTGCCGACTTGGATGATTGGAATAAGAGATTTTCAATGCTTTTGAAAGATTCTTTAAAGCAAGAAGTTATTTCCAGGGAGAAAAAAGATAGACGGGATTTTGATAAACTTGCTGCTCTGGCTACAACCCTGGGATTGTATAGTCATGCATATGCTAAGGTTGTTGTCTTCAGTAAGATCCCACTCCCCAACTATAGGTTTGATTTAGATGACAAGAAGCCACAGAGGGAGGTGAATTTGCATACTGACCTTCTTCAAAGAGTTGAAGCTTACCTCACAGAATACCTCAGTAAAAGCTCTAATAGGATAGATAGAGTCCCTGCCAATTCCGTCTCAAGAACTAGTAGCATTAGCAGTACTGATGAATGGTTTTCTGAGCAACCACTACCCATCTCTGCCACCAAAATTCTTTGGCAAAGGAGTTTGCAGCTGCGTGATCGACAACAATATTGGCAGGCATCCGTAGAAGGCCAAAGAATGTTGGATTCTCGTACAAGTCTCCCTGCTTTCAAGCAAAGGCATTCAGTATTGACAGCTATTTCACAAAATCAGGTTATTGTTATCTCTGGAGAAACAGGTTGCGGCAAGACCACACAAATCCCGCAGTTTATTTTAGAATCCGAGATTGAAGCAAACCGTGGAGCTTTTTCCAGTATCATATGTACTCAACCAAGGAGAATATCTGCTATGTCTGTTTCGGAGAGGGTTGCTTACGAGAGAGGAGAGCAGTTGGGTGAATCTGTTGGATATAAAGTTCGATTGGAAGGTGTTAAAGGAAGAGATACCCGCTTACTCTTCTGCACCACAGGCATTTTGTTGAGACGCTTGTTGGTAGATAGAAATTTAAGAGGAGTTACTCATGTTATCGTGGATGAAATTCATGAGCGTGGAATGAATGAAGATTTCCTTCTTATCATTCTTAAGGATCTGCTATCTCGTCGCTCAGAGCTTAAGCTGATTTTGATGAGTGCAACTTTAGATGCGGAGCTTTTCTCGTCATACTTTGGTGGGGCAGGTGTCATTTATATTCCAGGATTTACATATCCAGTTCGTAGTCATTTCCTAGAGGATATTCTGGAGATGACCCGGTACAGATTGACTCCATACAATCAAATTGATGATTATGGTCAAGAAAGAACGTGGAAGATGAACAAACAGATCCCAAAAAAACGGAAGAGCCAAATTACCTTTGTCGTTGAGGACGCACTGAGAGCAGCTGACTTCAAGGAGTTTAGCCCTGAGACTCGAGAGTCTTTATCTTGTTGGTATCCTGATTGCATTGGTTTTAACCTCATAGAATTTCTCCTTTGCAATATATGTGAGAATGAGGGTCCTGGTGGTATTTTAATTTTTTTGACTGGGTGGGATGATATCAGCTCTCTGAAAGAAAAACTGCAGATTCATCCGATCTTTGGCAACCCAGATTTGGTTATGTTGCTTGCCTGCCATGGTTCTATGGAAACTTTTGAGCAGAGGTTAATATTTGAAGAACCTGCAAGTGGAGTAAGAAAGATAGTCCTAGCCACTAATATCGCAGAAACGAGCATCACGATCAATGATGTTGCTTTTGTCATTGACTGTGGAAAGGCAAAAGAGACCTCCTATGATGCATTAAACAATACCCCCTGTTTGCTTCCTTCTTGGATTTCCAAGGTTTCAGCTCAGCAGAGAAGAGGAAGAGCCGGTCGTGTTCGACCTGGACAGTGTTACCATCTTTACCCAAAGTGCGTATATGATGCTTTTGCAGAATATCAGTTGCCTGAAATTTTAAGGACGCCTTTACACTCACTGTGCCTGCAAATCAAAAGCTTAAACCTCGGAAGCATATCCGAGTTCTTGTCAAGGGCGCTGCAATCTCCTGAACTACTAGCCGTTCAAAAGGCCATCGCATTTCTGAAGATAATTGGCGCATTGGATGAGAATGAAGACCTCACGACTCTAGGTCGTTATTTGTCCAAGCTTCCCATGGAGCCAAAGCTTGGAAAAATGCTCATACTAGGAGCTATTCTTGGTTGCCTTGATCCCATATTGACTGTTGCGGCTGGCCTGAGTGTGAGAGATCCTTTCTTGACACCTCAGGACAAGAAAGATCTCGCAGAAGCCGCAAAGTCCCAGTTTTCACGTGATCACAGTGATCATCTTGCACTTGTTCGGGCATATGAGGGGTGGAAAAAAGCTGAGGAAGAGTCTGCTGTATATGATTACTGCTGGAAAAACTTTCTGTCAATACAGTCGCTGAGAGCTATTGATTCTCTTCGAAAAGAGTTCTTCTCATTACTCAAAGATACTGGACTTATAGATGGAAACCCATCGATCTGCAATTCAGAGGGGAATGATGCAAATCTTACCCGAGCAGTTATTTGCTATGGCATGTATCCAGGAATCTGCTCAGTTGTGCATAATGAGAGATCATTTTCCCTGAAGACGATGGAGGATGGCCAAGTTCTTCTTTACTCAAATTCTGAAAATGCAAGGGAGACAAAAATACCATATCCGTGGTTGGTTTTCAACGAGAAGATAAAAGTGAACTCCGTTTTCTTGAGGGATTCGACAGCCTGCTCTGACTCTACTCTTATCCTCTTTGGCGGTAGCATCTCCAAAGGAGACACTGATGGCCACCTGAAAATGTTGGGAGGATACTTGGAGTTTTTCATGAAACCTGATGTTGCAGAGATATACCAAACCTTGAAGAAAGAGCTTGACGAGCTAATACAGAACAAACTACTTAATCCTAAAGTGGATATGCAAGCACACCGTGAACTCTTATCAGCTATACGGTTGTTGGTCTCTGAGGATGGATGTGATGGAAGATTTGTGTTTGGTCACCAAATACTTAGGCCTTTGGAAATATCGGCGTTGTCAACAAAACCGTCATTGTTTTCAAGGACCGAAAGTGGGCCGGGGGGTGACAATTCCAAAAGTCAGCTCCAAACAATACTCACGAGGGCTGGTTATACAGTACCGATGTATAAGACTAAGCAGTTGAAGAACAACAAGTTCCAAACAACAGTAGAGTTTAACGAGACACAGATCATGGGACAGCCTTGCAGCAACAAGAAGAGTGCTGAGAAGGATGCGGCAGCTGAAGCTATACAATGGCTGAAGGGAGGGGCTAAGGAAAGTCATGAGCAGGTGAATCATATGTCAAAGCTGCTGAAGAAAGGCAAGAAAGATCACCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0034641 GO:0042623 GO:0043170 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1113

Amino Acids

125.75

Weight (kDa)

7.7

Isoelectric Point (pI)

42.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	163 - 316	6e-10	DEAD/DEAH box helicase
Helicase_C	PF00271	424 - 552	2.3e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	616 - 643	6.9e-07	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	645 - 704	2.5e-15	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	772 - 853	2.6e-18	Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD	PF26026	861 - 919	4e-22	C-terminal domain in RNA helicases
dsrm	PF00035	961 - 1022	1.7e-11	Double-stranded RNA binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005622)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01130 AT2G01130
fragaria_vesca	FvH4_4g26440 FvH4_4g26440 FvH4_4g26440 FvH4_4g26440 FvH4_4g26440 FvH4_4g26440 FvH4_4g26440
malus_domestica	MD13G1033300.v1.1 MD16G1035100.v1.1
prunus_persica	Prupe.1G319600_v2.0.a1 Prupe.1G319600_v2.0.a1 Prupe.1G319600_v2.0.a1
pyrus_communis	pycom13g02870 pycom14g02420 pycom16g02990
rosa_chinensis	RchiOBHm_Chr4g0434071
rosa_laevigata	RLG00000006703
rosa_multiflora	Rmu_sc0000352.1_g000029
rosa_roxburghii	Rroxscaffold_5G00375480
rosa_rugosa	Rorug04G0273300 Rorug04G0273400
rosa_samantha	Rh4AG329000 Rh4BG336300 Rh4CG351700 Rh4DG332600
rosa_wichuraiana	Rw4G028520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1163, 3280
AasI	GACNNNNNNGTC	1 cut(s)	1408
Acc36I	ACCTGC	3 cut(s)	1163, 1660, 3280
Acc65I	GGTACC	1 cut(s)	149
AccB1I	GGYRCC	1 cut(s)	149
AccB7I	CCANNNNNTGG	1 cut(s)	2138
AccII	CGCG	1 cut(s)	120
AclWI	GGATC	6 cut(s)	138, 331, 1092, 1312, 2174, 2213
AcoI	YGGCCR	2 cut(s)	2596, 2773
AcsI	RAATTY	7 cut(s)	394, 564, 1000, 1035, 1457, 1923, 2617
AcuI	CTGAAG	6 cut(s)	313, 2075, 2600, 3264, 3281, 3335
AcvI	CACGTG	1 cut(s)	2282
AcyI	GRCGYC	1 cut(s)	1934
AdeI	CACNNNGTG	1 cut(s)	2915
AfaI	GTAC	7 cut(s)	29, 151, 517, 652, 823, 1250, 3120
AfiI	CCNNNNNNNGG	8 cut(s)	142, 146, 354, 1571, 1585, 1658, 2138, 3054
AflII	CTTAAG	2 cut(s)	1079, 1109
AhdI	GACNNNNNGTC	2 cut(s)	9, 2100
AhlI	ACTAGT	1 cut(s)	500
AjnI	CCWGG	6 cut(s)	233, 288, 1189, 1492, 1855, 2536
AloI	GAACNNNNNNTCC	2 cut(s)	897, 929
AlwI	GGATC	6 cut(s)	138, 331, 1092, 1312, 2174, 2213
AlwNI	CAGNNNCTG	1 cut(s)	2395
Ama87I	CYCGRG	2 cut(s)	1404, 2508
AoxI	GGCC	7 cut(s)	628, 2040, 2203, 2596, 2773, 2996, 3056
ApeKI	GCWGC	8 cut(s)	272, 586, 589, 1373, 2008, 3207, 3239, 3310
ApoI	RAATTY	7 cut(s)	394, 564, 1000, 1035, 1457, 1923, 2617
Asp718I	GGTACC	1 cut(s)	149
AspLEI	GCGC	3 cut(s)	48, 2008, 2069
AspS9I	GGNCC	3 cut(s)	1490, 3045, 3056
AsuC2I	CCSGG	2 cut(s)	1246, 3060
AsuHPI	GGTGA	7 cut(s)	32, 403, 905, 2975, 3077, 3304, 3326
AsuII	TTCGAA	1 cut(s)	2416
AvaI	CYCGRG	2 cut(s)	1404, 2508
AvaII	GGWCC	2 cut(s)	1490, 3045
AxyI	CCTNAGG	1 cut(s)	2235
BalI	TGGCCA	2 cut(s)	2598, 2775
BanI	GGYRCC	1 cut(s)	149
BauI	CACGAG	1 cut(s)	3100
BbrPI	CACGTG	1 cut(s)	2282
BbsI	GAAGAC	4 cut(s)	5, 319, 2091, 2588
BbvCI	CCTCAGC	1 cut(s)	2337
BbvI	GCAGC	8 cut(s)	259, 576, 598, 1385, 1995, 3219, 3251, 3297
BceAI	ACGGC	2 cut(s)	79, 2015
BciT130I	CCWGG	6 cut(s)	235, 290, 1191, 1494, 1857, 2538
BciVI	GTATCC	3 cut(s)	1437, 2544, 2789
BclI	TGATCA	2 cut(s)	2284, 2293
BcnI	CCSGG	2 cut(s)	1246, 3060
BcuI	ACTAGT	1 cut(s)	500
BfaI	CTAG	6 cut(s)	501, 1223, 1679, 2027, 2099, 2156
BfmI	CTRYAG	2 cut(s)	352, 1553
BfoI	RGCGCY	2 cut(s)	49, 2009
BfrI	CTTAAG	2 cut(s)	1079, 1109
BfuAI	ACCTGC	3 cut(s)	1163, 1660, 3280
BfuI	GTATCC	3 cut(s)	1437, 2544, 2789
BglII	AGATCT	1 cut(s)	2248
BlpI	GCTNAGC	1 cut(s)	1821
BmcAI	AGTACT	1 cut(s)	517
Bme1390I	CCNGG	8 cut(s)	235, 290, 1191, 1246, 1494, 1857, 2538, 3060
Bme18I	GGWCC	2 cut(s)	1490, 3045
BmeRI	GACNNNNNGTC	2 cut(s)	9, 2100
BmeT110I	CYCGRG	2 cut(s)	1404, 2508
BmgT120I	GGNCC	3 cut(s)	1490, 3045, 3056
BmiI	GGNNCC	4 cut(s)	55, 151, 1491, 2130
BmrFI	CCNGG	8 cut(s)	235, 290, 1191, 1246, 1494, 1857, 2538, 3060
BmrI	ACTGGG	2 cut(s)	1527, 2266
BmsI	GCATC	8 cut(s)	626, 1129, 1713, 1756, 1885, 2485, 2760, 3223
BmuI	ACTGGG	2 cut(s)	1527, 2266
BpiI	GAAGAC	4 cut(s)	5, 319, 2091, 2588
BplI	GAGNNNNNCTC	4 cut(s)	1136, 1168, 2414, 2446
BpmI	CTGGAG	3 cut(s)	72, 747, 1256
Bpu10I	CCTNAGC	3 cut(s)	315, 2337, 3273
Bpu1102I	GCTNAGC	1 cut(s)	1821
Bpu14I	TTCGAA	1 cut(s)	2416
BpuEI	CTTGAG	2 cut(s)	478, 2719
BpuMI	CCSGG	2 cut(s)	1246, 3060
Bsa29I	ATCGAT	1 cut(s)	2471
BsaAI	YACGTR	1 cut(s)	2282
BsaBI	GATNNNNATC	1 cut(s)	2499
BsaHI	GRCGYC	1 cut(s)	1934
BsaI	GGTCTC	2 cut(s)	1748, 2950
Bsc4I	CCNNNNNNNGG	8 cut(s)	142, 146, 354, 1571, 1585, 1658, 2138, 3054
Bse118I	RCCGGY	1 cut(s)	1841
Bse1I	ACTGG	6 cut(s)	89, 810, 1205, 1522, 2272, 2452
Bse21I	CCTNAGG	1 cut(s)	2235
Bse8I	GATNNNNATC	1 cut(s)	2499
BseBI	CCWGG	6 cut(s)	235, 290, 1191, 1494, 1857, 2538
BseCI	ATCGAT	1 cut(s)	2471
BseJI	GATNNNNATC	1 cut(s)	2499
BseLI	CCNNNNNNNGG	8 cut(s)	142, 146, 354, 1571, 1585, 1658, 2138, 3054
BseNI	ACTGG	6 cut(s)	89, 810, 1205, 1522, 2272, 2452
BseRI	GAGGAG	2 cut(s)	894, 1023
BseXI	GCAGC	8 cut(s)	259, 576, 598, 1385, 1995, 3219, 3251, 3297
BsgI	GTGCAG	1 cut(s)	943
Bsh1236I	CGCG	1 cut(s)	120
Bsh1285I	CGRYCG	1 cut(s)	1846
BshFI	GGCC	7 cut(s)	630, 2042, 2205, 2598, 2775, 2998, 3058
BshNI	GGYRCC	1 cut(s)	149
BshVI	ATCGAT	1 cut(s)	2471
BsiEI	CGRYCG	1 cut(s)	1846
BsiHKCI	CYCGRG	2 cut(s)	1404, 2508
BsiSI	CCGG	3 cut(s)	1246, 1842, 3059
BslFI	GGGAC	3 cut(s)	461, 2254, 3210
BslI	CCNNNNNNNGG	8 cut(s)	142, 146, 354, 1571, 1585, 1658, 2138, 3054
BsmBI	CGTCTC	2 cut(s)	496, 973
BsmFI	GGGAC	3 cut(s)	461, 2254, 3210
BsmI	GAATGC	1 cut(s)	679
BsnI	GGCC	7 cut(s)	630, 2042, 2205, 2598, 2775, 2998, 3058
Bso31I	GGTCTC	2 cut(s)	1748, 2950
BsoBI	CYCGRG	2 cut(s)	1404, 2508
Bsp119I	TTCGAA	1 cut(s)	2416
Bsp1720I	GCTNAGC	1 cut(s)	1821
Bsp19I	CCATGG	2 cut(s)	1606, 2124
BspANI	GGCC	7 cut(s)	630, 2042, 2205, 2598, 2775, 2998, 3058
BspDI	ATCGAT	1 cut(s)	2471
BspFNI	CGCG	1 cut(s)	120
BspHI	TCATGA	3 cut(s)	1039, 2811, 3283
BspLI	GGNNCC	4 cut(s)	55, 151, 1491, 2130
BspMAI	CTGCAG	1 cut(s)	1557
BspMI	ACCTGC	3 cut(s)	1163, 1660, 3280
BspPI	GGATC	6 cut(s)	138, 331, 1092, 1312, 2174, 2213
BspQI	GCTCTTC	2 cut(s)	1328, 1831
BspT104I	TTCGAA	1 cut(s)	2416
BspT107I	GGYRCC	1 cut(s)	149
BspTI	CTTAAG	2 cut(s)	1079, 1109
BspTNI	GGTCTC	2 cut(s)	1748, 2950
BsrFI	RCCGGY	1 cut(s)	1841
BsrI	ACTGG	6 cut(s)	89, 810, 1205, 1522, 2272, 2452
BssAI	RCCGGY	1 cut(s)	1841
BssNI	GRCGYC	1 cut(s)	1934
BssSI	CACGAG	1 cut(s)	3100
BssT1I	CCWWGG	5 cut(s)	49, 830, 1606, 1809, 2124
Bst2BI	CACGAG	1 cut(s)	3100
Bst2UI	CCWGG	6 cut(s)	235, 290, 1191, 1494, 1857, 2538
Bst6I	CTCTTC	8 cut(s)	931, 959, 1328, 1825, 1831, 2337, 2418, 3212
BstACI	GRCGYC	1 cut(s)	1934
BstAFI	CTTAAG	2 cut(s)	1079, 1109
BstBAI	YACGTR	1 cut(s)	2282
BstBI	TTCGAA	1 cut(s)	2416
BstC8I	GCNNGC	7 cut(s)	615, 1599, 1603, 1955, 2203, 2716, 2907
BstDSI	CCRYGG	4 cut(s)	796, 1606, 2124, 2654
BstEII	GGTNACC	1 cut(s)	2981
BstFNI	CGCG	1 cut(s)	120
BstH2I	RGCGCY	2 cut(s)	49, 2009
BstHHI	GCGC	3 cut(s)	48, 2008, 2069
BstMCI	CGRYCG	1 cut(s)	1846
BstMWI	GCNNNNNNNGC	7 cut(s)	43, 278, 966, 1370, 2261, 2863, 3245
BstNI	CCWGG	6 cut(s)	235, 290, 1191, 1494, 1857, 2538
BstNSI	RCATGY	1 cut(s)	2533
BstPI	GGTNACC	1 cut(s)	2981
BstSCI	CCNGG	8 cut(s)	233, 288, 1189, 1244, 1492, 1855, 2536, 3058
BstSFI	CTRYAG	2 cut(s)	352, 1553
BstUI	CGCG	1 cut(s)	120
BstV1I	GCAGC	8 cut(s)	259, 576, 598, 1385, 1995, 3219, 3251, 3297
BstV2I	GAAGAC	4 cut(s)	5, 319, 2091, 2588
BstX2I	RGATCY	5 cut(s)	336, 1084, 1317, 2218, 2248
BstXI	CCANNNNNNTGG	2 cut(s)	50, 2655
BstYI	RGATCY	5 cut(s)	336, 1084, 1317, 2218, 2248
Bsu15I	ATCGAT	1 cut(s)	2471
Bsu36I	CCTNAGG	1 cut(s)	2235
BsuI	GTATCC	3 cut(s)	1437, 2544, 2789
BsuRI	GGCC	7 cut(s)	630, 2042, 2205, 2598, 2775, 2998, 3058
BsuTUI	ATCGAT	1 cut(s)	2471
BtgI	CCRYGG	4 cut(s)	796, 1606, 2124, 2654
BtgZI	GCGATG	1 cut(s)	2029
BtsIMutI	CAGTG	6 cut(s)	157, 1364, 1868, 1946, 2296, 2766
BveI	ACCTGC	3 cut(s)	1163, 1660, 3280
Cac8I	GCNNGC	7 cut(s)	615, 1599, 1603, 1955, 2203, 2716, 2907
CaiI	CAGNNNCTG	1 cut(s)	2395
CciI	TCATGA	3 cut(s)	1039, 2811, 3283
CfoI	GCGC	3 cut(s)	48, 2008, 2069
Cfr10I	RCCGGY	1 cut(s)	1841
Cfr13I	GGNCC	3 cut(s)	1490, 3045, 3056
ClaI	ATCGAT	1 cut(s)	2471
CseI	GACGC	3 cut(s)	990, 1370, 1942
Csp6I	GTAC	7 cut(s)	28, 150, 516, 651, 822, 1249, 3119
CviAII	CATG	9 cut(s)	305, 1019, 1040, 1607, 2125, 2530, 2812, 3193, 3284
CviQI	GTAC	7 cut(s)	28, 150, 516, 651, 822, 1249, 3119
DraI	TTTAAA	1 cut(s)	216
DraIII	CACNNNGTG	1 cut(s)	2915
DrdI	GACNNNNNNGTC	1 cut(s)	1408
DriI	GACNNNNNGTC	2 cut(s)	9, 2100
DseDI	GACNNNNNNGTC	1 cut(s)	1408
EaeI	YGGCCR	2 cut(s)	2596, 2773
Eam1104I	CTCTTC	8 cut(s)	931, 959, 1328, 1825, 1831, 2337, 2418, 3212
Eam1105I	GACNNNNNGTC	2 cut(s)	9, 2100
EarI	CTCTTC	8 cut(s)	931, 959, 1328, 1825, 1831, 2337, 2418, 3212
EciI	GGCGGA	1 cut(s)	24
Eco130I	CCWWGG	5 cut(s)	49, 830, 1606, 1809, 2124
Eco147I	AGGCCT	1 cut(s)	2998
Eco31I	GGTCTC	2 cut(s)	1748, 2950
Eco32I	GATATC	1 cut(s)	1531
Eco47I	GGWCC	2 cut(s)	1490, 3045
Eco57I	CTGAAG	6 cut(s)	313, 2075, 2600, 3264, 3281, 3335
Eco72I	CACGTG	1 cut(s)	2282
Eco81I	CCTNAGG	1 cut(s)	2235
Eco88I	CYCGRG	2 cut(s)	1404, 2508
Eco91I	GGTNACC	1 cut(s)	2981
EcoO109I	RGGNCCY	1 cut(s)	1490
EcoO65I	GGTNACC	1 cut(s)	2981
EcoRII	CCWGG	6 cut(s)	233, 288, 1189, 1492, 1855, 2536
EcoRV	GATATC	1 cut(s)	1531
EcoT14I	CCWWGG	5 cut(s)	49, 830, 1606, 1809, 2124
EcoT22I	ATGCAT	2 cut(s)	310, 1771
ErhI	CCWWGG	5 cut(s)	49, 830, 1606, 1809, 2124
Esp3I	CGTCTC	2 cut(s)	496, 973
FaeI	CATG	9 cut(s)	308, 1022, 1043, 1610, 2128, 2533, 2815, 3196, 3287
FalI	AAGNNNNNCTT	6 cut(s)	1050, 1082, 1921, 1953, 2592, 2624
FaqI	GGGAC	3 cut(s)	461, 2254, 3210
FatI	CATG	9 cut(s)	304, 1018, 1039, 1606, 2124, 2529, 2811, 3192, 3283
FauI	CCCGC	2 cut(s)	768, 953
FauNDI	CATATG	4 cut(s)	310, 818, 2317, 3300
FbaI	TGATCA	2 cut(s)	2284, 2293
FspBI	CTAG	6 cut(s)	501, 1223, 1679, 2027, 2099, 2156
GlaI	GCGC	3 cut(s)	47, 2007, 2068
GsuI	CTGGAG	3 cut(s)	72, 747, 1256
HaeII	RGCGCY	2 cut(s)	49, 2009
HaeIII	GGCC	7 cut(s)	630, 2042, 2205, 2598, 2775, 2998, 3058
HapII	CCGG	3 cut(s)	1246, 1842, 3059
HgaI	GACGC	3 cut(s)	990, 1370, 1942
HhaI	GCGC	3 cut(s)	48, 2008, 2069
Hin1I	GRCGYC	1 cut(s)	1934
Hin1II	CATG	9 cut(s)	308, 1022, 1043, 1610, 2128, 2533, 2815, 3196, 3287
Hin6I	GCGC	3 cut(s)	46, 2006, 2067
HinP1I	GCGC	3 cut(s)	46, 2006, 2067
HincII	GTYRAC	1 cut(s)	3021
HindII	GTYRAC	1 cut(s)	3021
HindIII	AAGCTT	4 cut(s)	426, 1966, 2117, 2135
HpaII	CCGG	3 cut(s)	1246, 1842, 3059
HphI	GGTGA	7 cut(s)	32, 403, 905, 2975, 3077, 3304, 3326
Hpy166II	GTNNAC	3 cut(s)	2686, 2918, 3021
Hpy8I	GTNNAC	3 cut(s)	2686, 2918, 3021
Hpy99I	CGWCG	1 cut(s)	1101
HpyAV	CCTTC	8 cut(s)	30, 419, 620, 917, 1076, 1806, 3223, 3256
HpyCH4IV	ACGT	3 cut(s)	30, 1298, 2281
HpyF10VI	GCNNNNNNNGC	7 cut(s)	43, 278, 966, 1370, 2261, 2863, 3245
HpySE526I	ACGT	3 cut(s)	30, 1298, 2281
Hsp92I	GRCGYC	1 cut(s)	1934
Hsp92II	CATG	9 cut(s)	308, 1022, 1043, 1610, 2128, 2533, 2815, 3196, 3287
HspAI	GCGC	3 cut(s)	46, 2006, 2067
KpnI	GGTACC	1 cut(s)	153
Ksp22I	TGATCA	2 cut(s)	2284, 2293
LguI	GCTCTTC	2 cut(s)	1328, 1831
LmnI	GCTCC	5 cut(s)	800, 1144, 2128, 2159, 3090
Lsp1109I	GCAGC	8 cut(s)	259, 576, 598, 1385, 1995, 3219, 3251, 3297
LweI	GCATC	8 cut(s)	626, 1129, 1713, 1756, 1885, 2485, 2760, 3223
MaeI	CTAG	6 cut(s)	501, 1223, 1679, 2027, 2099, 2156
MaeII	ACGT	3 cut(s)	30, 1298, 2281
MaeIII	GTNAC	4 cut(s)	1012, 1865, 2981, 3065
MflI	RGATCY	5 cut(s)	336, 1084, 1317, 2218, 2248
MlsI	TGGCCA	2 cut(s)	2598, 2775
MluNI	TGGCCA	2 cut(s)	2598, 2775
MlyI	GAGTC	7 cut(s)	483, 1252, 1396, 1418, 2089, 2354, 2717
MmeI	TCCRAC	2 cut(s)	621, 883
Mox20I	TGGCCA	2 cut(s)	2598, 2775
Mph1103I	ATGCAT	2 cut(s)	310, 1771
MscI	TGGCCA	2 cut(s)	2598, 2775
MslI	CAYNNNNRTG	3 cut(s)	309, 1044, 2653
Msp20I	TGGCCA	2 cut(s)	2598, 2775
MspA1I	CMGCKG	3 cut(s)	589, 1376, 3242
MspCI	CTTAAG	2 cut(s)	1079, 1109
MspI	CCGG	3 cut(s)	1246, 1842, 3059
MspR9I	CCNGG	8 cut(s)	235, 290, 1191, 1246, 1494, 1857, 2538, 3060
Mva1269I	GAATGC	1 cut(s)	679
MvaI	CCWGG	6 cut(s)	235, 290, 1191, 1494, 1857, 2538
MvnI	CGCG	1 cut(s)	120
MwoI	GCNNNNNNNGC	7 cut(s)	43, 278, 966, 1370, 2261, 2863, 3245
NciI	CCSGG	2 cut(s)	1246, 3060
NcoI	CCATGG	2 cut(s)	1606, 2124
NdeI	CATATG	4 cut(s)	310, 818, 2317, 3300
NlaIII	CATG	9 cut(s)	308, 1022, 1043, 1610, 2128, 2533, 2815, 3196, 3287
NlaIV	GGNNCC	4 cut(s)	55, 151, 1491, 2130
NmuCI	GTSAC	2 cut(s)	2981, 3065
NsiI	ATGCAT	2 cut(s)	310, 1771
NspI	RCATGY	1 cut(s)	2533
NspV	TTCGAA	1 cut(s)	2416
PaeR7I	CTCGAG	1 cut(s)	1404
PagI	TCATGA	3 cut(s)	1039, 2811, 3283
PaqCI	CACCTGC	2 cut(s)	1163, 3280
PasI	CCCWGGG	1 cut(s)	289
PceI	AGGCCT	1 cut(s)	2998
PciSI	GCTCTTC	2 cut(s)	1328, 1831
PcsI	WCGNNNNNNNCGW	1 cut(s)	1706
PctI	GAATGC	1 cut(s)	679
PflMI	CCANNNNNTGG	1 cut(s)	2138
PfoI	TCCNGGA	2 cut(s)	1189, 2536
PleI	GAGTC	7 cut(s)	482, 1252, 1396, 1417, 2089, 2353, 2717
PmaCI	CACGTG	1 cut(s)	2282
PmlI	CACGTG	1 cut(s)	2282
PpsI	GAGTC	7 cut(s)	482, 1252, 1396, 1417, 2089, 2353, 2717
Ppu21I	YACGTR	1 cut(s)	2282
PpuMI	RGGWCCY	1 cut(s)	1490
Psp5II	RGGWCCY	1 cut(s)	1490
Psp6I	CCWGG	6 cut(s)	233, 288, 1189, 1492, 1855, 2536
PspCI	CACGTG	1 cut(s)	2282
PspEI	GGTNACC	1 cut(s)	2981
PspGI	CCWGG	6 cut(s)	233, 288, 1189, 1492, 1855, 2536
PspN4I	GGNNCC	4 cut(s)	55, 151, 1491, 2130
PspPI	GGNCC	3 cut(s)	1490, 3045, 3056
PspPPI	RGGWCCY	1 cut(s)	1490
PstI	CTGCAG	1 cut(s)	1557
PstNI	CAGNNNCTG	1 cut(s)	2395
PsuI	RGATCY	5 cut(s)	336, 1084, 1317, 2218, 2248
PvuII	CAGCTG	3 cut(s)	589, 1376, 3242
RsaI	GTAC	7 cut(s)	29, 151, 517, 652, 823, 1250, 3120
RsaNI	GTAC	7 cut(s)	28, 150, 516, 651, 822, 1249, 3119
RseI	CAYNNNNRTG	3 cut(s)	309, 1044, 2653
SapI	GCTCTTC	2 cut(s)	1328, 1831
Sau96I	GGNCC	3 cut(s)	1490, 3045, 3056
ScaI	AGTACT	1 cut(s)	517
SchI	GAGTC	7 cut(s)	483, 1252, 1396, 1418, 2089, 2354, 2717
ScrFI	CCNGG	8 cut(s)	235, 290, 1191, 1246, 1494, 1857, 2538, 3060
SfaNI	GCATC	8 cut(s)	626, 1129, 1713, 1756, 1885, 2485, 2760, 3223
SfcI	CTRYAG	2 cut(s)	352, 1553
Sfr274I	CTCGAG	1 cut(s)	1404
SfuI	TTCGAA	1 cut(s)	2416
SinI	GGWCC	2 cut(s)	1490, 3045
SlaI	CTCGAG	1 cut(s)	1404
SmiMI	CAYNNNNRTG	3 cut(s)	309, 1044, 2653
SmlI	CTYRAG	5 cut(s)	493, 1079, 1109, 1404, 2698
SmoI	CTYRAG	5 cut(s)	493, 1079, 1109, 1404, 2698
SpeI	ACTAGT	1 cut(s)	500
SseBI	AGGCCT	1 cut(s)	2998
SspI	AATATT	2 cut(s)	608, 1641
SspMI	CTAG	6 cut(s)	501, 1223, 1679, 2027, 2099, 2156
StuI	AGGCCT	1 cut(s)	2998
StyD4I	CCNGG	8 cut(s)	233, 288, 1189, 1244, 1492, 1855, 2536, 3058
StyI	CCWWGG	5 cut(s)	49, 830, 1606, 1809, 2124
TaiI	ACGT	3 cut(s)	33, 1301, 2284
TaqI	TCGA	7 cut(s)	598, 916, 1405, 1852, 2416, 2471, 2708
TaqII	GACCGA	1 cut(s)	3062
TatI	WGTACW	2 cut(s)	515, 821
TauI	GCSGC	4 cut(s)	744, 2201, 2264, 3239
TscAI	CASTG	6 cut(s)	164, 1371, 1868, 1953, 2296, 2773
TseFI	GTSAC	2 cut(s)	2981, 3065
TseI	GCWGC	8 cut(s)	272, 586, 589, 1373, 2008, 3207, 3239, 3310
Tsp45I	GTSAC	2 cut(s)	2981, 3065
TspGWI	ACGGA	5 cut(s)	478, 610, 1345, 2643, 2680
TspRI	CASTG	6 cut(s)	164, 1371, 1868, 1953, 2296, 2773
Van91I	CCANNNNNTGG	1 cut(s)	2138
Vha464I	CTTAAG	2 cut(s)	1079, 1109
VpaK11BI	GGWCC	2 cut(s)	1490, 3045
XapI	RAATTY	7 cut(s)	394, 564, 1000, 1035, 1457, 1923, 2617
XceI	RCATGY	1 cut(s)	2533
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2122
XhoI	CTCGAG	1 cut(s)	1404
XspI	CTAG	6 cut(s)	501, 1223, 1679, 2027, 2099, 2156
ZrmI	AGTACT	1 cut(s)	517
Zsp2I	ATGCAT	2 cut(s)	310, 1771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.