Rorug04G0273400

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005622
Physical Location & Seq
Reverse (-)
44597480 .. 44601419
3940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0273400.1

Sequence Viewer

Length: 579 bp
ATGAACAGTGCCGGCTCCAGTCGAGCCGATATAGTAAAAGACAATTTTGCCCCCGCCTGCGTTTCTTGCCTCGACACCTGGCGTACTGCTCAGTCCTATCGTGTCTTCGCTACTTCAATCCACAGAGAGGTGGAGAGAGAGAGAGAGAAACAGCGAAGGGGACTGAAATCGGAGAGGAGGAAGGGAGAATTTATCAAGGCCAAGGAAGGTATTGCTTCTTCTTGCTCTGGGTGGTTGTTTGTGAATTTTGCAGAATGGAATAGCAGAGAAGAATTGGTGGGGGAGAGTTTGGGTTGGGTAAGCTGCCAGGGGTTTGATTGTTTTCGTTGGGATCGACAATGGTTAGTAGTAAACTACAAAGGGAGGGCATATGCTCAAAGAGTTGAGCCAAAGCTTGCTTTGGTTGAGGTACAGCTGCCAAATGAGGCATTCCTAGAGAATTGGGAACCTACTAAAAGCTCTTATCTAGTATTAAAAGCACCTGGGATGAATGTGCTGAAGATTTCCTTGAGTACACCGCAAGAAATAGCAGATGGGGTTTCAGTATGGGAGTGGTCTGGTGCTGCGCTAGATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

192

Amino Acids

21.84

Weight (kDa)

7.59

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MOSC_N PF03476 100 - 191 7.7e-15 MOSC N-terminal beta barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 54, 518
AclWI GGATC 1 cut(s) 339
AcsI RAATTY 2 cut(s) 188, 244
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 3 cut(s) 85, 411, 514
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 1 cut(s) 117
AjnI CCWGG 3 cut(s) 77, 306, 481
AluBI AGCT 4 cut(s) 303, 394, 415, 459
AluI AGCT 4 cut(s) 303, 394, 415, 459
AlwI GGATC 1 cut(s) 339
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 3 cut(s) 303, 415, 563
ApoI RAATTY 2 cut(s) 188, 244
AspLEI GCGC 1 cut(s) 568
BarI GAAGNNNNNNTAC 2 cut(s) 202, 234
BbsI GAAGAC 1 cut(s) 97
BbvI GCAGC 3 cut(s) 290, 402, 550
BccI CCATC 1 cut(s) 527
BciT130I CCWGG 3 cut(s) 79, 308, 483
BfaI CTAG 3 cut(s) 434, 467, 569
BisI GCNGC 3 cut(s) 304, 416, 564
BlsI GCNGC 3 cut(s) 305, 417, 565
Bme1390I CCNGG 3 cut(s) 79, 308, 483
BmiI GGNNCC 2 cut(s) 16, 447
BmrFI CCNGG 3 cut(s) 79, 308, 483
BpiI GAAGAC 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 529
BsaJI CCNNGG 3 cut(s) 201, 307, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 1 cut(s) 18
BseBI CCWGG 3 cut(s) 79, 308, 483
BseDI CCNNGG 3 cut(s) 201, 307, 482
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 1 cut(s) 190
BseXI GCAGC 3 cut(s) 290, 402, 550
BshFI GGCC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 1 cut(s) 200
Bsp143I GATC 1 cut(s) 331
BspACI CCGC 2 cut(s) 54, 518
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 16, 447
BspPI GGATC 1 cut(s) 339
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 3 cut(s) 201, 307, 482
BssMI GATC 1 cut(s) 331
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 3 cut(s) 79, 308, 483
Bst4CI ACNGT 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 13, 58, 396
BstDEI CTNAG 1 cut(s) 90
BstF5I GGATG 1 cut(s) 492
BstHHI GCGC 1 cut(s) 568
BstKTI GATC 1 cut(s) 334
BstMBI GATC 1 cut(s) 331
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstNI CCWGG 3 cut(s) 79, 308, 483
BstSCI CCNGG 3 cut(s) 77, 306, 481
BstV1I GCAGC 3 cut(s) 290, 402, 550
BstV2I GAAGAC 1 cut(s) 97
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 3 cut(s) 13, 58, 396
CfoI GCGC 1 cut(s) 568
Cfr10I RCCGGY 1 cut(s) 11
Csp6I GTAC 3 cut(s) 84, 410, 513
CviJI RGCY 8 cut(s) 15, 26, 200, 303, 388, 394, 415, 459
CviKI_1 RGCY 8 cut(s) 15, 26, 200, 303, 388, 394, 415, 459
CviQI GTAC 3 cut(s) 84, 410, 513
DdeI CTNAG 1 cut(s) 90
DpnI GATC 1 cut(s) 333
DpnII GATC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 518
EcoRII CCWGG 3 cut(s) 77, 306, 481
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaiI YATR 4 cut(s) 32, 370, 372, 547
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 1 cut(s) 174
FauI CCCGC 1 cut(s) 61
FauNDI CATATG 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 304, 416, 564
FokI GGATG 1 cut(s) 499
Fsp4HI GCNGC 3 cut(s) 304, 416, 564
FspBI CTAG 3 cut(s) 434, 467, 569
GlaI GCGC 1 cut(s) 567
GluI GCNGC 3 cut(s) 304, 416, 564
HaeIII GGCC 1 cut(s) 200
HapII CCGG 1 cut(s) 12
HhaI GCGC 1 cut(s) 568
Hin6I GCGC 1 cut(s) 566
HinP1I GCGC 1 cut(s) 566
HindIII AAGCTT 1 cut(s) 392
HpaII CCGG 1 cut(s) 12
Hpy166II GTNNAC 2 cut(s) 352, 515
Hpy188I TCNGA 1 cut(s) 172
Hpy8I GTNNAC 2 cut(s) 352, 515
HpyAV CCTTC 3 cut(s) 150, 175, 200
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4V TGCA 1 cut(s) 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 1 cut(s) 90
HspAI GCGC 1 cut(s) 566
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
Kzo9I GATC 1 cut(s) 331
LmnI GCTCC 1 cut(s) 20
Lsp1109I GCAGC 3 cut(s) 290, 402, 550
MaeI CTAG 3 cut(s) 434, 467, 569
MalI GATC 1 cut(s) 333
MboI GATC 1 cut(s) 331
MboII GAAGA 4 cut(s) 97, 210, 281, 511
MluCI AATT 6 cut(s) 43, 188, 244, 272, 439, 574
MnlI CCTC 7 cut(s) 80, 121, 168, 171, 357, 400, 418
MroNI GCCGGC 1 cut(s) 11
MseI TTAA 2 cut(s) 473, 577
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 3 cut(s) 79, 308, 483
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 3 cut(s) 79, 308, 483
MwoI GCNNNNNNNGC 1 cut(s) 66
NaeI GCCGGC 1 cut(s) 13
NdeI CATATG 1 cut(s) 370
NdeII GATC 1 cut(s) 331
NgoMIV GCCGGC 1 cut(s) 11
NlaIV GGNNCC 2 cut(s) 16, 447
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PctI GAATGC 1 cut(s) 428
PdiI GCCGGC 1 cut(s) 13
PkrI GCNGC 3 cut(s) 305, 417, 565
Psp6I CCWGG 3 cut(s) 77, 306, 481
PspGI CCWGG 3 cut(s) 77, 306, 481
PspN4I GGNNCC 2 cut(s) 16, 447
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 3 cut(s) 85, 411, 514
RsaNI GTAC 3 cut(s) 84, 410, 513
SaqAI TTAA 2 cut(s) 473, 577
SatI GCNGC 3 cut(s) 304, 416, 564
Sau3AI GATC 1 cut(s) 331
ScrFI CCNGG 3 cut(s) 79, 308, 483
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 6 cut(s) 43, 188, 244, 272, 439, 574
SsiI CCGC 2 cut(s) 54, 518
SspMI CTAG 3 cut(s) 434, 467, 569
StyD4I CCNGG 3 cut(s) 77, 306, 481
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 8
TaqI TCGA 3 cut(s) 22, 72, 334
TasI AATT 6 cut(s) 43, 188, 244, 272, 439, 574
TatI WGTACW 1 cut(s) 512
Tru1I TTAA 2 cut(s) 473, 577
Tru9I TTAA 2 cut(s) 473, 577
TscAI CASTG 1 cut(s) 13
TseI GCWGC 3 cut(s) 303, 415, 563
TspDTI ATGAA 2 cut(s) 17, 503
TspRI CASTG 1 cut(s) 13
XapI RAATTY 2 cut(s) 188, 244
XspI CTAG 3 cut(s) 434, 467, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.