Rh4BG336300

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005622
Physical Location & Seq
Reverse (-)
49947012 .. 49956152
9141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG336300.1

Sequence Viewer

Length: 2889 bp
ATGCCCTCTTCACCATCTCCAATTCTTCTTCTTCTCCATCCCTCACCTCCTTCTCCACAATCACAATTCCCTTGGCGTCCCAAAGCCTCTGCCCTCAGACCCTCACCACTACTCGTCGCCATGAAGGACCGGCCTCCTTCTTCGTACGGCGCCGTTTACGTCCCTCCCCACCACCGCCTCCGCTCCGTCATCACCAGCCCTAATTACACCTCCGCCGCCTCAATTGCCTCCAAGAAGACTACTGCTGCTCCTCTCAGCAAAGCTCGCACCAATGGTGCTCGGACCTACTACCAGACTCAGCAGCACGAAGTTCAGGAACAGCTCCACAAGCCGAAGTTGCAGCACGATTCTGCTTACGACGGCGGCGGCGTCTCCGATGATGAAGGCTCCGATCGCGATTATAACATGTCGTCGAATCCGGGTGCTTCAATCTCTGATAACCTTGATGAGTGGACACGGAAGTTAACCATGCTGTTACGTGACAACAAGAAGCAAGAGTTGGTTTCAAGGGAGAAAAAAGATAGACGTGATTTTGAGGACATAGCCGCTTTGGCAAGCAGAATGGGACTTTACAGCCATCTGTATGCAAAAGTTGCTGTATTCAGTAAGGTCCCACTGCCAAACTACAGATTTGATCTGGATGACCGGCGACCTCAGAGAGAGGTGACCTTGCCTCTTGGGTTGCTGAGGAGAGTTGAGGCGTACCTCGGAGACTTCCTTTCTCAGAAGTCTAGGACTAAGGAAACCTTTCCAGATGTTTCCTTTTCTAGATCAAGCAGTAGTGGCAGTATTGGTACTGATGAAGGGCTCTTTGAGCAACCTGAACCAGTGGCACCGAATAATGCTGCGATGGAAAAAGTTCTTTGGAGGAGAAGCTTGCAGCTGCGGGATAAGCAACACGCTTGGCAGGAATCTCGTGAAGGCAGAAAAGTTATGGAACTTCGTAGAAGTCTCCCAGCTTATAAAGAGAAGGATGCTTTGTTGACAGCAATTTCACGAAATCAGGTTGTTATCATATCAGGGCAAACAGGCTGTGGAAAGACCACTCAAATTCCACAATTCATTTTAGAATCTGAGATAGAAGCTGCCCGTGGAGCTGTATGTAGTATTATATGTACACAGCCTAGGCGAATATCTGCTATGTCTGTTTCAGAAAGAGTTGCTTCAGAGAGAGGGGAGAAGTTGGGTGATTCAGTTGGATATAAGGTTCGGTTAGAGGGCATGAAAGGGAGAGACACTCGCCTTCTCTTCTGCACCACTGGCATTTTGTTGAGAAGATTGCTACTTGATGGAAATTTGAAAGGTGTAACTCATGTCATTGTGGATGAGATTCACGAACGCGGAATGAATGAAGATTTTCTGCTTATTGTTCTCAAGGATCTCCTTCCTCGCCGGCCAGAACTGAGGTTGATTCTGATGAGTGCAACCCTAGATTCTGAGCTTTTCTCATCCTACTTTGGCAGGGCTCAGATAATTCATGTTCCAGGTTTTACATACCCTGTTCGAACTCATTTTCTGGAGGATGTTCTGGAAAGTACCGGTTGCAGGTTAACTCCATACAATCAGATTGATGATTATGGACAAGAAAAGATGTGGAAAATGAGTAAACAGGCACCAAGAAAGAGGAAAAGTCAAATTGCTTCTGTTGTTGAGGATGCACTCAAAGCAGCTGATTTTAAGGGTTACAGTCCCCAGACTCGGGAATCTCTAGCCTGTTGGAATCCTGATTGTATTGGTTTTAATCTCATAGAGTATCTGTTATGCAATATCTGTGAGAATGAAAGGCCTGGTGCCATTCTAGTATTTATGACTGGGTGGGATGACATCAATTCTCTAAAGGAAAAGTTGCATGCAAATCCTCTCTTAGGTGATCCAAGCCAAGTCTTGTTGTTAGCATGCCATGGTTCCATGGCCAGTTCCGAGCAGAGGTTAATATTTGATGAGCCTGAGGATGGAGTGAGGAAAATAGTGCTGGCTACTAATATTGCTGAGACGAGTATCACAATTAATGACGTTGTTTTTGTTATTGATTGTGGAAAGGCAAAAGAGACTTCATATGATGCACTGAATAATACTCCTTGTTTGCTTCCTTCCTGGATATCCAAGGTTTCTGCTCAACAAAGAAGAGGAAGAGCTGGGCGCGTTCAACCTGGGGAATGTTATCGTCTCTATCCTAGATGTGTGTATGATGCATTTGCAGAATATCAATTGCCAGAAATCTTGAGGACACCTTTGCAGTCTCTTTGTCTGCAAATCAAAAGTTTAAAACTTGGAAGTATCTCAGAGTTCTTATCTAGGGCTCTACAGTCACCAGAGTTACTTGCGGTGAAAAATGCCATAGAGTATTTAAAGATCATTGGGGCCTTGGATGAGAACGAGAATCTGACTATTTTAGGACGCTATCTAACTATGCTTCCTGTGGAGCCAAAACTTGGAAAGATGCTCTTGGTCGGTGCCATCTTCAACTGCCTCGATCCTGTTTTGACTATTGTTTCTGGCCTTAGTGTCAGAGATCCTTTCTTAACACCATTTGACAAGAAAGATCTTGCGGAGGCTGCTAAATCTCAGTTTTCCCGAGACCACAGCGATCACCTTGCACTTGTGCGTGCCTATGAGGGCTGGAAGGTAGCTGAAAGAGACTTTGCTGGATATGACTACTGCTGGAAAAATTTTCTTTCAGCACAATCGATGAAAGCTATTGATTCTCTCAGGAAGGAGTTCTACTCTTTGCTAAGGGATACTGATCTTATCGATGCCAACACAGCTACCTATAATGTATGGAGCTATGATGAGCATCTCGTCCGTGCAGTTATTTGCTATGGCCTGTATCCTGGAATTAGCTCTGTTATGGTAAGCGTGGTTTTTTCCTATGTTTTCTTTAAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

962

Amino Acids

108.21

Weight (kDa)

8.5

Isoelectric Point (pI)

52.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 328 - 480 8.2e-11 DEAD/DEAH box helicase
Helicase_C PF00271 587 - 715 6.5e-13 Helicase conserved C-terminal domain
WHD_HA2 PF04408 779 - 807 4.7e-06 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 808 - 867 3.8e-16 Helicase associated domain (HA2), ratchet-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 402, 963
Acc36I ACCTGC 1 cut(s) 1536
AccB1I GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
AccB7I CCANNNNNTGG 1 cut(s) 2432
AccBSI CCGCTC 1 cut(s) 183
AccII CGCG 3 cut(s) 396, 1341, 2142
AclWI GGATC 4 cut(s) 1386, 1865, 2468, 2507
AcoI YGGCCR 2 cut(s) 1394, 1911
AcsI RAATTY 3 cut(s) 1050, 1294, 2668
AcuI CTGAAG 1 cut(s) 1149
AcyI GRCGYC 3 cut(s) 76, 150, 369
AfaI GTAC 5 cut(s) 146, 704, 796, 1117, 1537
AfiI CCNNNNNNNGG 7 cut(s) 1545, 1698, 1699, 1865, 1926, 1952, 2432
AflIII ACRYGT 1 cut(s) 405
AgeI ACCGGT 1 cut(s) 1538
AgsI TTSAA 5 cut(s) 429, 507, 1300, 2147, 2464
AjiI CACGTC 1 cut(s) 527
AjnI CCWGG 5 cut(s) 1483, 1786, 2093, 2149, 2830
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AloI GAACNNNNNNTCC 2 cut(s) 1191, 1223
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 4 cut(s) 1386, 1865, 2468, 2507
Ama87I CYCGRG 2 cut(s) 1698, 2574
AoxI GGCC 7 cut(s) 131, 1394, 1784, 1911, 2361, 2497, 2821
ApeKI GCWGC 9 cut(s) 245, 301, 340, 845, 880, 883, 1085, 1667, 2555
ApoI RAATTY 3 cut(s) 1050, 1294, 2668
ArsI GACNNNNNNTTYG 2 cut(s) 2003, 2035
AseI ATTAAT 1 cut(s) 2007
AsiGI ACCGGT 1 cut(s) 1538
Asp700I GAANNNNTTC 4 cut(s) 747, 858, 1356, 2717
AspA2I CCTAGG 1 cut(s) 1124
AspLEI GCGC 2 cut(s) 152, 2142
AspS9I GGNCC 4 cut(s) 127, 282, 610, 2361
AsuC2I CCSGG 1 cut(s) 420
AsuII TTCGAA 1 cut(s) 1504
AvaI CYCGRG 2 cut(s) 1698, 2574
AvaII GGWCC 3 cut(s) 127, 282, 610
AvrII CCTAGG 1 cut(s) 1124
AxyI CCTNAGG 1 cut(s) 1947
BalI TGGCCA 1 cut(s) 1913
BanI GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
BanII GRGCYC 3 cut(s) 810, 1468, 2302
BarI GAAGNNNNNNTAC 2 cut(s) 2705, 2737
BauI CACGAG 1 cut(s) 915
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 280
BbvCI CCTCAGC 1 cut(s) 686
BbvI GCAGC 9 cut(s) 232, 313, 352, 832, 870, 892, 1072, 1679, 2542
BccI CCATC 7 cut(s) 22, 45, 585, 844, 1283, 1946, 2465
BceAI ACGGC 3 cut(s) 137, 163, 376
BcgI CGANNNNNNTGC 4 cut(s) 896, 930, 2576, 2610
BciT130I CCWGG 5 cut(s) 1485, 1788, 2095, 2151, 2832
BciVI GTATCC 2 cut(s) 2731, 2838
BcnI CCSGG 1 cut(s) 420
BfaI CTAG 8 cut(s) 732, 768, 1125, 1430, 1709, 1799, 2175, 2295
BfmI CTRYAG 2 cut(s) 625, 2303
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 1536
BfuI GTATCC 2 cut(s) 2731, 2838
BglI GCCNNNNNGGC 1 cut(s) 551
BglII AGATCT 1 cut(s) 2542
BlnI CCTAGG 1 cut(s) 1124
Bme1390I CCNGG 6 cut(s) 420, 1485, 1788, 2095, 2151, 2832
Bme18I GGWCC 3 cut(s) 127, 282, 610
BmeT110I CYCGRG 2 cut(s) 1698, 2574
BmgBI CACGTC 1 cut(s) 527
BmgT120I GGNCC 4 cut(s) 127, 282, 610, 2361
BmrFI CCNGG 6 cut(s) 420, 1485, 1788, 2095, 2151, 2832
BmrI ACTGGG 1 cut(s) 1821
BmsI GCATC 7 cut(s) 964, 1645, 2050, 2179, 2430, 2743, 2803
BmuI ACTGGG 1 cut(s) 1821
BpiI GAAGAC 1 cut(s) 242
BplI GAGNNNNNCTC 6 cut(s) 1222, 1254, 1430, 1462, 2708, 2740
BpmI CTGGAG 1 cut(s) 1538
Bpu10I CCTNAGC 2 cut(s) 686, 2732
Bpu14I TTCGAA 1 cut(s) 1504
BpuEI CTTGAG 2 cut(s) 1358, 2242
BpuMI CCSGG 1 cut(s) 420
Bsa29I ATCGAT 2 cut(s) 2687, 2751
BsaAI YACGTR 1 cut(s) 479
BsaBI GATNNNNATC 2 cut(s) 2742, 2793
BsaHI GRCGYC 3 cut(s) 76, 150, 369
BsaI GGTCTC 1 cut(s) 2571
BsaJI CCNNGG 9 cut(s) 71, 706, 1090, 1124, 1900, 1908, 2103, 2150, 2364
BsaWI WCCGGW 1 cut(s) 1538
BsaXI ACNNNNNCTCC 2 cut(s) 232, 262
Bsc4I CCNNNNNNNGG 7 cut(s) 1545, 1698, 1699, 1865, 1926, 1952, 2432
Bse118I RCCGGY 4 cut(s) 129, 645, 1392, 1538
Bse1I ACTGG 4 cut(s) 827, 1264, 1816, 1914
Bse21I CCTNAGG 1 cut(s) 1947
Bse8I GATNNNNATC 2 cut(s) 2742, 2793
BseBI CCWGG 5 cut(s) 1485, 1788, 2095, 2151, 2832
BseCI ATCGAT 2 cut(s) 2687, 2751
BseDI CCNNGG 9 cut(s) 71, 706, 1090, 1124, 1900, 1908, 2103, 2150, 2364
BseJI GATNNNNATC 2 cut(s) 2742, 2793
BseLI CCNNNNNNNGG 7 cut(s) 1545, 1698, 1699, 1865, 1926, 1952, 2432
BseNI ACTGG 4 cut(s) 827, 1264, 1816, 1914
BseRI GAGGAG 3 cut(s) 240, 703, 883
BseXI GCAGC 9 cut(s) 232, 313, 352, 832, 870, 892, 1072, 1679, 2542
BseYI CCCAGC 2 cut(s) 955, 2135
BsgI GTGCAG 2 cut(s) 1237, 2826
Bsh1236I CGCG 3 cut(s) 396, 1341, 2142
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 7 cut(s) 133, 1396, 1786, 1913, 2363, 2499, 2823
BshNI GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
BshTI ACCGGT 1 cut(s) 1538
BshVI ATCGAT 2 cut(s) 2687, 2751
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 280
BsiHKCI CYCGRG 2 cut(s) 1698, 2574
BsiSI CCGG 5 cut(s) 130, 419, 646, 1393, 1539
BsiWI CGTACG 1 cut(s) 144
BslFI GGGAC 5 cut(s) 63, 146, 579, 596, 1674
BslI CCNNNNNNNGG 7 cut(s) 1545, 1698, 1699, 1865, 1926, 1952, 2432
BsmBI CGTCTC 3 cut(s) 376, 1985, 2171
BsmFI GGGAC 5 cut(s) 63, 146, 579, 596, 1674
BsnI GGCC 7 cut(s) 133, 1396, 1786, 1913, 2363, 2499, 2823
Bso31I GGTCTC 1 cut(s) 2571
BsoBI CYCGRG 2 cut(s) 1698, 2574
Bsp119I TTCGAA 1 cut(s) 1504
Bsp1286I GDGCHC 4 cut(s) 280, 810, 1468, 2302
Bsp1407I TGTACA 1 cut(s) 1115
Bsp19I CCATGG 2 cut(s) 1900, 1908
Bsp68I TCGCGA 1 cut(s) 396
BspANI GGCC 7 cut(s) 133, 1396, 1786, 1913, 2363, 2499, 2823
BspDI ATCGAT 2 cut(s) 2687, 2751
BspFNI CGCG 3 cut(s) 396, 1341, 2142
BspMI ACCTGC 1 cut(s) 1536
BspPI GGATC 4 cut(s) 1386, 1865, 2468, 2507
BspQI GCTCTTC 1 cut(s) 2125
BspT104I TTCGAA 1 cut(s) 1504
BspT107I GGYRCC 5 cut(s) 149, 832, 1612, 1790, 2453
BspTNI GGTCTC 1 cut(s) 2571
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 4 cut(s) 129, 645, 1392, 1538
BsrGI TGTACA 1 cut(s) 1115
BsrI ACTGG 4 cut(s) 827, 1264, 1816, 1914
BssAI RCCGGY 4 cut(s) 129, 645, 1392, 1538
BssECI CCNNGG 9 cut(s) 71, 706, 1090, 1124, 1900, 1908, 2103, 2150, 2364
BssNI GRCGYC 3 cut(s) 76, 150, 369
BssSI CACGAG 1 cut(s) 915
BssT1I CCWWGG 6 cut(s) 71, 1124, 1900, 1908, 2103, 2364
Bst2BI CACGAG 1 cut(s) 915
Bst2UI CCWGG 5 cut(s) 1485, 1788, 2095, 2151, 2832
Bst4CI ACNGT 2 cut(s) 1688, 2307
Bst6I CTCTTC 4 cut(s) 13, 1253, 2119, 2125
BstACI GRCGYC 3 cut(s) 76, 150, 369
BstAPI GCANNNNNTGC 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 1115
BstBAI YACGTR 1 cut(s) 479
BstBI TTCGAA 1 cut(s) 1504
BstC8I GCNNGC 8 cut(s) 265, 556, 878, 1394, 1851, 1897, 1974, 2607
BstDSI CCRYGG 3 cut(s) 1090, 1900, 1908
BstEII GGTNACC 1 cut(s) 664
BstENI CCTNNNNNAGG 1 cut(s) 1863
BstFNI CGCG 3 cut(s) 396, 1341, 2142
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 2 cut(s) 152, 2142
BstMCI CGRYCG 1 cut(s) 394
BstNI CCWGG 5 cut(s) 1485, 1788, 2095, 2151, 2832
BstNSI RCATGY 3 cut(s) 409, 1853, 1899
BstPI GGTNACC 1 cut(s) 664
BstSCI CCNGG 6 cut(s) 418, 1483, 1786, 2093, 2149, 2830
BstSFI CTRYAG 2 cut(s) 625, 2303
BstUI CGCG 3 cut(s) 396, 1341, 2142
BstV1I GCAGC 9 cut(s) 232, 313, 352, 832, 870, 892, 1072, 1679, 2542
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 3 cut(s) 1378, 2512, 2542
BstYI RGATCY 3 cut(s) 1378, 2512, 2542
Bsu15I ATCGAT 2 cut(s) 2687, 2751
Bsu36I CCTNAGG 1 cut(s) 1947
BsuI GTATCC 2 cut(s) 2731, 2838
BsuRI GGCC 7 cut(s) 133, 1396, 1786, 1913, 2363, 2499, 2823
BsuTUI ATCGAT 2 cut(s) 2687, 2751
BtgI CCRYGG 3 cut(s) 1090, 1900, 1908
BtgZI GCGATG 1 cut(s) 863
BtrI CACGTC 1 cut(s) 527
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 4 cut(s) 614, 834, 1257, 2063
BtuMI TCGCGA 1 cut(s) 396
BveI ACCTGC 1 cut(s) 1536
Cac8I GCNNGC 8 cut(s) 265, 556, 878, 1394, 1851, 1897, 1974, 2607
CfoI GCGC 2 cut(s) 152, 2142
Cfr10I RCCGGY 4 cut(s) 129, 645, 1392, 1538
Cfr13I GGNCC 4 cut(s) 127, 282, 610, 2361
ClaI ATCGAT 2 cut(s) 2687, 2751
CseI GACGC 3 cut(s) 65, 358, 2406
Csp6I GTAC 5 cut(s) 145, 703, 795, 1116, 1536
CspAI ACCGGT 1 cut(s) 1538
CviQI GTAC 5 cut(s) 145, 703, 795, 1116, 1536
DinI GGCGCC 1 cut(s) 151
DraI TTTAAA 2 cut(s) 2265, 2349
EaeI YGGCCR 2 cut(s) 1394, 1911
Eam1104I CTCTTC 4 cut(s) 13, 1253, 2119, 2125
EarI CTCTTC 4 cut(s) 13, 1253, 2119, 2125
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 6 cut(s) 71, 1124, 1900, 1908, 2103, 2364
Eco147I AGGCCT 1 cut(s) 1786
Eco24I GRGCYC 3 cut(s) 810, 1468, 2302
Eco31I GGTCTC 1 cut(s) 2571
Eco32I GATATC 1 cut(s) 2100
Eco47I GGWCC 3 cut(s) 127, 282, 610
Eco57I CTGAAG 1 cut(s) 1149
Eco81I CCTNAGG 1 cut(s) 1947
Eco88I CYCGRG 2 cut(s) 1698, 2574
Eco91I GGTNACC 1 cut(s) 664
EcoNI CCTNNNNNAGG 1 cut(s) 1863
EcoO109I RGGNCCY 2 cut(s) 610, 2361
EcoO65I GGTNACC 1 cut(s) 664
EcoRII CCWGG 5 cut(s) 1483, 1786, 2093, 2149, 2830
EcoRV GATATC 1 cut(s) 2100
EcoT14I CCWWGG 6 cut(s) 71, 1124, 1900, 1908, 2103, 2364
EcoT22I ATGCAT 1 cut(s) 2194
EcoT38I GRGCYC 3 cut(s) 810, 1468, 2302
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 6 cut(s) 71, 1124, 1900, 1908, 2103, 2364
Esp3I CGTCTC 3 cut(s) 376, 1985, 2171
FalI AAGNNNNNCTT 6 cut(s) 731, 763, 1147, 1179, 2429, 2461
FaqI GGGAC 5 cut(s) 63, 146, 579, 596, 1674
FauI CCCGC 1 cut(s) 879
FauNDI CATATG 1 cut(s) 2056
FriOI GRGCYC 3 cut(s) 810, 1468, 2302
FspBI CTAG 8 cut(s) 732, 768, 1125, 1430, 1709, 1799, 2175, 2295
GlaI GCGC 2 cut(s) 151, 2141
GsaI CCCAGC 2 cut(s) 959, 2139
GsuI CTGGAG 1 cut(s) 1538
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 7 cut(s) 133, 1396, 1786, 1913, 2363, 2499, 2823
HapII CCGG 5 cut(s) 130, 419, 646, 1393, 1539
HgaI GACGC 3 cut(s) 65, 358, 2406
HhaI GCGC 2 cut(s) 152, 2142
Hin1I GRCGYC 3 cut(s) 76, 150, 369
Hin6I GCGC 2 cut(s) 150, 2140
HinP1I GCGC 2 cut(s) 150, 2140
HincII GTYRAC 3 cut(s) 465, 984, 1551
HindII GTYRAC 3 cut(s) 465, 984, 1551
HindIII AAGCTT 1 cut(s) 874
HpaI GTTAAC 2 cut(s) 465, 1551
HpaII CCGG 5 cut(s) 130, 419, 646, 1393, 1539
Hpy166II GTNNAC 7 cut(s) 157, 453, 465, 984, 1118, 1551, 1607
Hpy8I GTNNAC 7 cut(s) 157, 453, 465, 984, 1118, 1551, 1607
Hpy99I CGWCG 3 cut(s) 119, 362, 415
HpyCH4III ACNGT 2 cut(s) 1688, 2307
HpyCH4IV ACGT 4 cut(s) 159, 478, 526, 2013
HpySE526I ACGT 4 cut(s) 159, 478, 526, 2013
Hsp92I GRCGYC 3 cut(s) 76, 150, 369
HspAI GCGC 2 cut(s) 150, 2140
KasI GGCGCC 1 cut(s) 149
KroI GCCGGC 1 cut(s) 1392
KroNI GCCGGC 1 cut(s) 1394
KspAI GTTAAC 2 cut(s) 465, 1551
LguI GCTCTTC 1 cut(s) 2125
LmnI GCTCC 7 cut(s) 188, 253, 327, 392, 1094, 2422, 2781
Lsp1109I GCAGC 9 cut(s) 232, 313, 352, 832, 870, 892, 1072, 1679, 2542
LweI GCATC 7 cut(s) 964, 1645, 2050, 2179, 2430, 2743, 2803
MaeI CTAG 8 cut(s) 732, 768, 1125, 1430, 1709, 1799, 2175, 2295
MaeII ACGT 4 cut(s) 159, 478, 526, 2013
MaeIII GTNAC 7 cut(s) 474, 479, 664, 1306, 1682, 2307, 2316
MbiI CCGCTC 1 cut(s) 183
MfeI CAATTG 2 cut(s) 222, 2207
MflI RGATCY 3 cut(s) 1378, 2512, 2542
MhlI GDGCHC 4 cut(s) 280, 810, 1468, 2302
MlsI TGGCCA 1 cut(s) 1913
MluNI TGGCCA 1 cut(s) 1913
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 2 cut(s) 289, 1690
MmeI TCCRAC 2 cut(s) 1177, 1697
Mox20I TGGCCA 1 cut(s) 1913
Mph1103I ATGCAT 1 cut(s) 2194
MroNI GCCGGC 1 cut(s) 1392
MroXI GAANNNNTTC 4 cut(s) 747, 858, 1356, 2717
MscI TGGCCA 1 cut(s) 1913
MslI CAYNNNNRTG 1 cut(s) 582
Msp20I TGGCCA 1 cut(s) 1913
MspA1I CMGCKG 2 cut(s) 883, 1670
MspI CCGG 5 cut(s) 130, 419, 646, 1393, 1539
MspR9I CCNGG 6 cut(s) 420, 1485, 1788, 2095, 2151, 2832
MunI CAATTG 2 cut(s) 222, 2207
MvaI CCWGG 5 cut(s) 1485, 1788, 2095, 2151, 2832
MvnI CGCG 3 cut(s) 396, 1341, 2142
NaeI GCCGGC 1 cut(s) 1394
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 1 cut(s) 420
NcoI CCATGG 2 cut(s) 1900, 1908
NdeI CATATG 1 cut(s) 2056
NgoMIV GCCGGC 1 cut(s) 1392
NmuCI GTSAC 3 cut(s) 479, 664, 2307
NruI TCGCGA 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 2194
NspI RCATGY 3 cut(s) 409, 1853, 1899
NspV TTCGAA 1 cut(s) 1504
PaeI GCATGC 2 cut(s) 1853, 1899
PceI AGGCCT 1 cut(s) 1786
PciI ACATGT 1 cut(s) 405
PciSI GCTCTTC 1 cut(s) 2125
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PdiI GCCGGC 1 cut(s) 1394
PdmI GAANNNNTTC 4 cut(s) 747, 858, 1356, 2717
Pfl23II CGTACG 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 2432
PfoI TCCNGGA 2 cut(s) 2093, 2830
PinAI ACCGGT 1 cut(s) 1538
Ple19I CGATCG 1 cut(s) 394
PleI GAGTC 2 cut(s) 289, 1690
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 2 cut(s) 289, 1690
Ppu21I YACGTR 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 610
PscI ACATGT 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 2007
PsiI TTATAA 2 cut(s) 402, 963
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 5 cut(s) 1483, 1786, 2093, 2149, 2830
PspEI GGTNACC 1 cut(s) 664
PspFI CCCAGC 2 cut(s) 955, 2135
PspGI CCWGG 5 cut(s) 1483, 1786, 2093, 2149, 2830
PspLI CGTACG 1 cut(s) 144
PspPI GGNCC 4 cut(s) 127, 282, 610, 2361
PspPPI RGGWCCY 1 cut(s) 610
PsuI RGATCY 3 cut(s) 1378, 2512, 2542
PvuI CGATCG 1 cut(s) 394
PvuII CAGCTG 2 cut(s) 883, 1670
RruI TCGCGA 1 cut(s) 396
RsaI GTAC 5 cut(s) 146, 704, 796, 1117, 1537
RsaNI GTAC 5 cut(s) 145, 703, 795, 1116, 1536
RseI CAYNNNNRTG 1 cut(s) 582
SapI GCTCTTC 1 cut(s) 2125
Sau96I GGNCC 4 cut(s) 127, 282, 610, 2361
SchI GAGTC 2 cut(s) 289, 1690
ScrFI CCNGG 6 cut(s) 420, 1485, 1788, 2095, 2151, 2832
SduI GDGCHC 4 cut(s) 280, 810, 1468, 2302
SfaNI GCATC 7 cut(s) 964, 1645, 2050, 2179, 2430, 2743, 2803
SfcI CTRYAG 2 cut(s) 625, 2303
SfoI GGCGCC 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 1504
SinI GGWCC 3 cut(s) 127, 282, 610
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 1373, 2221
SmoI CTYRAG 2 cut(s) 1373, 2221
SphI GCATGC 2 cut(s) 1853, 1899
SseBI AGGCCT 1 cut(s) 1786
SspDI GGCGCC 1 cut(s) 149
SspI AATATT 2 cut(s) 1935, 1984
SspMI CTAG 8 cut(s) 732, 768, 1125, 1430, 1709, 1799, 2175, 2295
StuI AGGCCT 1 cut(s) 1786
StyD4I CCNGG 6 cut(s) 418, 1483, 1786, 2093, 2149, 2830
StyI CCWWGG 6 cut(s) 71, 1124, 1900, 1908, 2103, 2364
TaaI ACNGT 2 cut(s) 1688, 2307
TaiI ACGT 4 cut(s) 162, 481, 529, 2016
TaqI TCGA 5 cut(s) 413, 1504, 2472, 2687, 2751
TatI WGTACW 1 cut(s) 1115
TauI GCSGC 4 cut(s) 218, 366, 369, 548
TscAI CASTG 4 cut(s) 621, 834, 1264, 2070
TseFI GTSAC 3 cut(s) 479, 664, 2307
TseI GCWGC 9 cut(s) 245, 301, 340, 845, 880, 883, 1085, 1667, 2555
Tsp45I GTSAC 3 cut(s) 479, 664, 2307
TspGWI ACGGA 3 cut(s) 175, 472, 2792
TspRI CASTG 4 cut(s) 621, 834, 1264, 2070
Van91I CCANNNNNTGG 1 cut(s) 2432
VpaK11BI GGWCC 3 cut(s) 127, 282, 610
VspI ATTAAT 1 cut(s) 2007
XagI CCTNNNNNAGG 1 cut(s) 1863
XapI RAATTY 3 cut(s) 1050, 1294, 2668
XbaI TCTAGA 1 cut(s) 767
XceI RCATGY 3 cut(s) 409, 1853, 1899
XmaJI CCTAGG 1 cut(s) 1124
XmnI GAANNNNTTC 4 cut(s) 747, 858, 1356, 2717
XspI CTAG 8 cut(s) 732, 768, 1125, 1430, 1709, 1799, 2175, 2295
Zsp2I ATGCAT 1 cut(s) 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.